| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Here we report the cloning , by either peptide sequencing or by sequence similarity to the human cDNAs , of the S . cerevisiae genes RFC 1 , RFC 2 , RFC 3 , RFC 4 , and RFC 5 . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Cloning and characterization of the essential Saccharomyces cerevisiae RFC 4 gene encoding the 37 kDa subunit of replication factor C . ^^^ The RFC 4 gene encoding the 37 kDa subunit of Saccharomyces cerevisiae replication factor C ( RF C ) has been cloned and sequenced . ^^^ The RFC 4 gene encodes a 323 amino acid polypeptide with a molecular weight of 36 , 126 . ^^^ The deduced amino acid sequence of the RFC 4 gene shows high sequence similarity to the other two small subunits of yeast RF C and the three small subunits of human RF C ( 35 60 % identity , 55 78 % similarity ) . ^^^ Despite the presence of a MluI cell cycle box in the regulatory region of the RFC 4 gene , the steady state levels of its mRNA do not change significantly during the yeast mitotic cell cycle . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| These experiments showed that the small RFC proteins , Rfc 2 , Rfc 3 , Rfc 4 and Rfc 5 , interacted with Rad 24 , whereas the Rfc 1 subunit did not . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Rad 24 is structurally related to replication factor C ( RFC ) subunits and associates with RFC subunits Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . rad24Delta mutants are defective in all the G ( 1 ) , S , and G ( 2 ) / M phase DNA damage checkpoints , whereas the rfc 5 1 mutant is impaired only in the S phase DNA damage checkpoint . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Rfc 4 interacts with Rpa 1 and is required for both DNA replication and DNA damage checkpoints in Saccharomyces cerevisiae . ^^^ A mutation in RFC 4 , encoding a small subunit of replication factor C ( RFC ) , was found to display allele specific interactions with mutations in the gene encoding Rpa 1 ( RFA 1 ) . ^^^ Mutations that map to Rpa1N and confer sensitivity to the DNA synthesis inhibitor hydroxyurea , such as rfa 1 t11 , are lethal in combination with rfc 4 2 . ^^^ The rfc 4 2 mutant itself is sensitive to hydroxyurea , and like rfc 2 and rfc 5 strains , it exhibits defects in the DNA replication block and intra S checkpoints . ^^^ RFC 4 and the DNA damage checkpoint gene RAD 24 were found to be epistatic with respect to DNA damage sensitivity . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| The ATP binding domains of Rfc 2 , Rfc 3 , and Rfc 4 are essential for DNA recognition and clamp loading . ^^^ The conserved lysine in the Walker A motif of the ATP binding domain encoded by the yeast RFC 1 , RFC 2 , RFC 3 , and RFC 4 genes was mutated to glutamic acid . ^^^ A mutant complex containing the rfc 4 K55E mutation performed as well as a wild type complex in clamp loading , but only at very high ATP concentrations . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Rad 24 is structurally related to subunits of the replication factor C ( RFC ) complex , and forms an RFC related complex with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ We also show that Chl 12 interacts physically with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 and forms an RFC related complex which is distinct from the RFC and RAD 24 complexes . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| To determine the relative importance of these sites for proper DNA metabolism in the cell , the conserved lysine in the Walker A motif of RFC 1 , RFC 2 , RFC 3 , or RFC 4 was mutated to either arginine or glutamic acid . ^^^ A glutamic acid substitution resulted in lethality in RFC 2 and RFC 3 but not in RFC 1 or RFC 4 . ^^^ Except for the rfc 1 K359R and rfc 4 K55E mutants , which were phenotypically similar to wild type in every assay , the mutants were sensitive to DNA damaging agents . ^^^ The rfc 2 K71R and rfc 4 K55R mutants show checkpoint defects , most likely in the intra S phase checkpoint . ^^^ These data demonstrate that the ATP binding function of RFC 2 is important for both DNA replication and checkpoint function and , for the first time , that RFC 4 also plays a role in checkpoint regulation . . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| We find that the RFC ( 3 / 4 ) complex is a DNA dependent ATPase , and we use this activity to determine that RFC 3 supplies a catalytic arginine to the ATP site of RFC 4 . ^^^ Furthermore , the RFC ( 2 / 3 ) and RFC ( 3 / 4 ) subassemblies bind stably to PCNA , yet neither RFC 2 nor RFC 4 bind tightly to PCNA , indicating that RFC 3 forms a strong contact point to PCNA . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Proteins required for this pathway include Rad 17 , Rad 9 , Hus 1 , Rad 1 , and Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| In that system , three ATP molecules bound to the Rfc 2 , Rfc 3 , and Rfc 4 subunits are necessary and sufficient for efficient loading of PCNA , whereas ATP binding to Rfc 1 is not required . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Furthermore , temperature sensitive mutations in RFC 4 result in precocious sister chromatid separation . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Loading of PCNA onto DNA at template primer junctions is performed in an ATP dependent process by replication factor C ( RFC ) , a heteropentameric AAA+ protein complex consisting of the Rfc 1 , Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 subunits . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Similar results were obtained with antisera to the clamp loader proteins Rfc 3 and Rfc 4 , and to PCNA , i . e . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| Furthermore , RFC 37 , the third subunit of the RFC complex , competes with RIalpha and displaces it from the RFC 40 RIalpha complex . ^^^ |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P35251 and P35249 |
Pubmed |
SVM Score :0.0 |
| NA |
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