| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| GRK 6 is the most recently identified member of the GRK family and displays higher homology with GRK 5 ( 70 . 1 % amino acid identity ) and IT 11 ( 68 . 5 % ) compared to beta ARK ( 37 . 4 % ) and rhodopsin kinase ( 47 . 1 % ) . ^^^ Rhodopsin and the beta 2 adrenergic and m 2 muscarinic cholinergic receptors serve as stimulus dependent substrates for GRK 6 , but with stoichiometries significantly lower than achieved by GRK 5 and beta ARK . ^^^ Additionally , GRK 6 does not undergo significant autophosphorylation even though it contains residues identical to those that are autophosphorylated in GRK 5 and rhodopsin kinase . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| Purified GRK 5 phosphorylates rhodopsin in a light dependent manner and beta 2 adrenergic receptor in an agonist dependent manner and phosphorylates the C terminal tail regions of both receptor proteins . ^^^ GRK 5 possesses neither a CAAX motif specifying protein prenylation like rhodopsin kinase nor similarity to the G protein beta gamma subunit binding domain of beta adrenergic receptor kinases . ^^^ GRK 5 phosphorylation of rhodopsin or beta 2 adrenergic receptor is not stimulated by G protein beta gamma subunits . ^^^ The GRK 5 protein does not undergo agonist dependent translocation from cytosol to membranes as do beta adrenergic receptor kinase and rhodopsin kinase , but rather appears to associate with membranes constitutively . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| Expression and characterization of a mutant GRK 5 that does not autophosphorylate ( S484A and T485A ) reveals that the mutant has a approximately 15 20 fold reduced ability to phosphorylate the beta 2 adrenergic receptor and rhodopsin compared to wild type GRK 5 . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| Overexpression of rhodopsin kinase , beta ARK 1 , beta ARK 2 , or GRK 5 significantly increases agonist induced beta 1AR phosphorylation and concomitantly decreases agonist stimulated cellular cAMP production ( p < 0 . 05 ) . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| Here we report the isolation of a cDNA that encodes a 590 amino acid protein kinase , termed GRK 5 , which has 34 . 8 % and 47 . 2 % amino acid identities with beta ARK and rhodopsin kinase , respectively . ^^^ GRK 5 , overexpressed in Sf 9 insect cells using the baculovirus system , was able to phosphorylate rhodopsin in a light dependent manner . ^^^ In addition , GRK 5 neither contains a consensus sequence for isoprenylation like rhodopsin kinase nor is activated by G protein beta gamma subunits like beta ARK 1 . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| The family of G protein coupled receptor kinases includes rhodopsin kinase , beta ARK 1 , beta ARK 2 , IT 11 A ( GRK 4 ) , GRK 5 , and GRK 6 . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| Last , we show that mutation of the hydrophobic residues severely diminishes phospholipid dependent autophosphorylation of GRK 5 and phosphorylation of membrane bound rhodopsin by GRK 5 . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| The CC regions of GRK 1 , GRK 2 , and GRK 5 , representatives of the three subfamilies of GRKs , could bind rhodopsin in vitro and inhibit GRK 2 mediated phosphorylation of rhodopsin , but not a peptide GRK substrate . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| GRK 5 is a recently identified member of the GRK family that has greater homology with rhodopsin kinase than with beta ARK . ^^^ GRK 5 phosphorylates the beta 2 adrenergic receptor ( beta 2AR ) , m 2 muscarinic cholinergic receptor , and rhodopsin in an agonist dependent manner to maximal stoichiometries of approximately 2 . 5 , 1 . 5 , and 1 mol of phosphate / mol of receptor , respectively , with Km values of approximately 0 . 5 microM for the beta 2AR , approximately 16 microM for rhodopsin , and approximately 24 microM for ATP . ^^^ Peptide phosphorylation studies suggest that in contrast to beta ARK and rhodopsin kinase , GRK 5 preferentially phosphorylates on nonacidic peptides with a Km of approximately 1 . 5 mM . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| Here we report the isolation of a cDNA that encodes a 576 amino acid protein kinase , termed GRK 6 , that has significant homology with GRK 5 ( 70 . 1 % amino acid identity ) , IT 11 ( 68 . 5 % ) , rhodopsin kinase ( 47 . 1 % ) , and beta ARK ( 37 . 4 % ) . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| The location of multiple GRK 2 and GRK 5 phosphoacceptor sites at the extreme carboxyl terminus of the beta2AR is highly reminiscent of GRK 1 mediated phosphorylation of rhodopsin . . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| GRK 5 phosphorylation by PKC dramatically reduces its ability to phosphorylate both receptor ( light activated rhodopsin ) and non receptor ( casein and phosvitin ) substrates . ^^^ The reduced affinity of PKC phosphorylated GRK 5 for rhodopsin was also evident in a decreased ability to bind to rhodopsin containing membranes , while direct binding of GRK 5 to phospholipids appeared unaltered . ^^^ |
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| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08100 and P34947 |
Pubmed |
SVM Score :0.0 |
| NA |
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