| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| A serine residue at position 411 in the tail of the beta 2 adrenergic receptor is a substrate for phosphorylation by GRK 5 ( for G protein coupled receptor kinase 5 ) and is required for interaction with EBP 50 and for proper recycling of the receptor . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| Purified GRK 5 phosphorylates rhodopsin in a light dependent manner and beta 2 adrenergic receptor in an agonist dependent manner and phosphorylates the C terminal tail regions of both receptor proteins . ^^^ GRK 5 phosphorylation of rhodopsin or beta 2 adrenergic receptor is not stimulated by G protein beta gamma subunits . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| Expression and characterization of a mutant GRK 5 that does not autophosphorylate ( S484A and T485A ) reveals that the mutant has a approximately 15 20 fold reduced ability to phosphorylate the beta 2 adrenergic receptor and rhodopsin compared to wild type GRK 5 . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| Enriched membrane preparations of the beta 2 and alpha 2 C 2 adrenergic receptors ( ARs , where alpha 2 C 2 AR refers to the AR whose gene is located on human chromosome 2 ) prepared by sucrose density gradient centrifugation from Sf 9 or 293 cells contain the receptor at 100 300 pmol / mg of protein and serve as efficient substrates for agonist dependent phosphorylation by beta AR kinase 1 ( GRK 2 ) , beta AR kinase 2 ( GRK 3 ) , or GRK 5 . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| GRK 5 phosphorylates the beta 2 adrenergic receptor ( beta 2AR ) , m 2 muscarinic cholinergic receptor , and rhodopsin in an agonist dependent manner to maximal stoichiometries of approximately 2 . 5 , 1 . 5 , and 1 mol of phosphate / mol of receptor , respectively , with Km values of approximately 0 . 5 microM for the beta 2AR , approximately 16 microM for rhodopsin , and approximately 24 microM for ATP . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| Here we report the identification of the sites of GRK 2 and GRK 5 mediated beta2AR phosphorylation . ^^^ The location of multiple GRK 2 and GRK 5 phosphoacceptor sites at the extreme carboxyl terminus of the beta2AR is highly reminiscent of GRK 1 mediated phosphorylation of rhodopsin . . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| Impaired hormonal stimulation of AC , associated with beta AR uncoupling , may result from increased GRK 5 expression . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| Tentative identification of the G protein coupled receptor kinase 2 and 5 ( GRK 2 and GRK 5 ) sites of phosphorylation of the beta 2 adrenergic receptor ( betaAR ) was recently reported based on in vitro phosphorylation of recombinant receptor ( Fredericks , Z . ^^^ |
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| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| Therefore , we generated hybrid transgenic mice with myocardium targeted overexpression of 1 of 3 GRKs expressed in the heart ( GRK 2 [ commonly known as the beta AR kinase 1 ] , GRK 3 , or GRK 5 ) with concomitant cardiac expression of a constitutively activated mutant ( CAM ) or wild type alpha ( 1B ) AR . ^^^ |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34947 and P07550 |
Pubmed |
SVM Score :0.0 |
| NA |
|