| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| HsMcm 10 also interacted with Orc 2 , Mcm 2 and Mcm 6 proteins in the yeast two hybrid system . ^^^ These results suggest that HsMcm 10 may function in DNA replication through the interaction with Orc and Mcm 2 7 complexes . . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| In addition , cdc 24 ( + ) genetically interacts with the gene encoding the catalytic subunit of DNA polymerase epsilon , which is stimulated by PCNA and RFC , and with those encoding the fission yeast counterparts of Mcm 2 , Mcm 4 , and Mcm 10 . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| Furthermore , diminished interaction between Mcm 10 and Mcm 7 , a subunit of the MCM 2 7 complex , by a mutation in either Mcm 10 or Mcm 7 inhibits replication initiation . ^^^ Surprisingly , a double mutant containing both the mcm 10 1 and mcm 7 1 ( cdc 47 1 ) alleles restores interaction between Mcm 10 and Mcm 7 and corrects all of the defects exhibited by each of the single mutants , including the stalling of replication forks at replication origins typically seen in mcm 10 1 cells . ^^^ These results suggest that Mcm 10 , like Mcm 7 , is a critical component of the prereplication chromatin and that interaction between Mcm 10 and Mcm 7 is required for proper replication initiation and prompt release of origin bound factors . . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| Characterization of Schizosaccharomyces pombe mcm 7 ( + ) and cdc 23 ( + ) ( MCM 10 ) and interactions with replication checkpoints . ^^^ We isolated a novel temperature sensitive allele , mcm 7 98 , and also characterized two temperature sensitive alleles of the fission yeast homolog of MCM 10 , cdc 23 ( + ) . mcm 7 98 and both cdc23ts alleles arrest with damaged chromosomes and an S phase delay . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| Moreover , Mcm 10 interacts with key members of the prereplication complex : Mcm 2 , Dup ( Cdt 1 ) , and Orc 2 . ^^^ Furthermore , depletion of Mcm 10 , Cdc 45 , Mcm 2 , Mcm 5 , and Orc 2 , respectively , results in aberrant chromosome condensation . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| These observations show that Cdc23 / Mcm10 function is conserved between fission yeast and Xenopus , where in vitro analysis has indicated a similar requirement for Cdc 45 binding , but apparently not compared with Saccharomyces cerevisiae , where Mcm 10 is needed for Mcm 2 chromatin binding . ^^^ However , unlike the situation in Xenopus , where Mcm 10 chromatin binding is dependent on Mcm 2 7 , we show that the fission yeast protein is bound to chromatin throughout the cell cycle in growing cells , and only displaced from chromatin during quiescence . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| We find that the pre replication complex ( pre RC ) components Cdc 6 and Mcm 7 associate with ARS 1 in the mcm 10 1 mutant , suggesting that establishment of the pre RC is not compromised in this mutant . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| Suppressor mutations in MCM 2 , which suppress the temperature sensitivity of mcm 10 1 , fail to overcome the mcm 10 1 silencing defect , suggesting that MCM 10 ' s role in transcriptional silencing may be separate from its essential functions in DNA replication . . ^^^ |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q7L590 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
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