| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Protein hCdc 21 forms a stable trimeric complex with two novel human Mcm proteins , p85Mcm and p105Mcm . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| We detected in HeLa cell extracts six proteins , CDC 47 , CDC46 / MCM5 , Cdc 21 , P1 / MCM3 , Mis 5 , and BM28 / MCM2 , by their binding to a specific antibody and by partial sequencing . ^^^ The human homologs of the MCM 2 ( BM 28 ) , Mis 5 , Cdc 21 , and CDC 47 proteins were tightly bound to a histone Sepharose column and purified to near homogeneity , whereas the P1 / MCM3 and CDC46 / MCM5 proteins passed through . ^^^ Among the four core histones , the human BM28 / MCM2 , Mis 5 , Cdc 21 , and CDC 47 proteins had high affinity for histone H 3 . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| This gene family includes Saccharomyces cerevisiae MCM 2 , MCM 3 , MCM5 / CDC46 and CDC 47 , S . pombe nda 1 , nda 4 , cdc 21 and mis 5 , and genes encoding human BM 28 , P1MCM3 and P1 . 1MCM3 and mouse P1MCM3 , most of which are considered to be required for the initiation of DNA replication . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The members of the MCM protein family , including MCM 2 , MCM 3 , Cdc 21 , CDC 46 , Mis 5 and CDC 47 , are considered to be involved in the control of a single round of DNA replication during S phase in eukaryotes . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Our group purified MCM protein complexes consisting of MCM 2 , 4 ( Cdc 21 ) , 6 ( Mis 5 ) , and 7 ( CDC 47 ) proteins from HeLa cells by using histone Sepharose column chromatography ( Ishimi , Y . , Ichinose , S . , Omori , A . , Sato K . , and Kimura , H . ( 1996 ) J . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| We compared the effects of disruptions of cdc 19 ( + ) ( mcm 2 ( + ) ) , cdc 21 ( + ) ( mcm 4 ( + ) ) , nda 4 ( + ) ( mcm 5 ( + ) ) and mis 5 ( + ) ( mcm 6 ( + ) ) ; in all cases , the null mutants underwent delayed S phase but were unable to proceed through the cell cycle . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| DNA cloning and expression during development of Drosophila melanogaster MCM 3 , MCM 6 and MCM 7 . cDNAs encoding three Drosophila melanogaster MCM proteins , DmMCM 3 , DmMCM 6 and DmMCM 7 , candidates of DNA replication licensing factors , were cloned and sequenced . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| In this study , we have expressed and isolated the mouse Mcm 2 , 4 , 6 , 7 proteins from insect cells and characterized various mutant Mcm 4 , 6 , 7 complexes in which the conserved ATPase motifs of the Mcm 4 and Mcm 6 proteins were mutated . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Following exit from mitosis , the Mcm 4 subunit of the cytosolic interphase complex undergoes dephosphorylation , and the Mcm 2 , Mcm 3 , or Mcm 6 subunits are then actively phosphorylated by kinase ( s ) other than cyclin dependent kinases ( Cdks ) or Cdc 7 . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| HsMcm 10 also interacted with Orc 2 , Mcm 2 and Mcm 6 proteins in the yeast two hybrid system . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Finally , using protein protein cross linking we have shown that MCM 2 interacts directly with MCM 5 and MCM 6 ; MCM 5 with MCM 3 and MCM 2 ; and MCM 6 with MCM 2 and MCM 4 . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Cdc 23 shows genetic interactions with four MCM subunits : cdc 23 M36 and cdc 23 1E2 alleles both show synthetic phenotypes with mcm 2 ( cdc 19 P1 ) and mcm 6 ( mis 5 268 ) , and cdc 23 M36 is synthetically lethal with mcm 4 ( cdc 21 K46 ) and with mcm 5 ( nda 4 108 ) . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Biol . 19 , 8003 8015 ) , our data indicate distinct roles of Mcm 4 , Mcm 6 , and Mcm 7 subunits in activation of the DNA helicase activity of the Mcm4 / 6 / 7 complex . . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Stimulation of quiescent rat aortic vascular smooth muscle cells with fetal bovine serum induced MCM 6 and MCM 7 protein and mRNA expression , which was potently attenuated by atorvastatin in a dose dependent fashion . ^^^ Mevalonate completely abrogated the inhibitory effect on serum induced MCM 6 and MCM 7 expression , demonstrating that biosynthesis of isoprenoids was likely the specific pathway blocked by atorvastatin . ^^^ Transient transfection experiments revealed that atorvastatin inhibited MCM 6 and MCM 7 promoter activity , implicating a transcriptional mechanism . ^^^ The MCM 6 and MCM 7 promoters contain several E2F sites critical for their transcriptional activation . ^^^ The inhibitory effect of atorvastatin on MCM 6 and MCM 7 was reversed by adenoviral mediated overexpression of E2F , indicating that their downregulation by atorvastatin involves an E2F dependent mechanism . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Rapamycin substantially inhibited mitogen induced MCM 6 and MCM 7 mRNA and protein expression in a dose dependent fashion . ^^^ Transient transfection experiments revealed that rapamycin inhibited MCM 6 and MCM 7 promoter activity , implicating a transcriptional mechanism . ^^^ MCM 6 and MCM 7 transcriptional activation is regulated by E2F and activity of a luciferase reporter plasmid driven by four E2F elements was also significantly inhibited by rapamycin . ^^^ The inhibitory effect of rapamycin on MCM 6 and MCM 7 was reversed by overexpression of E2F , indicating that their downregulation by rapamycin involves an E2F dependent mechanism . ^^^ These observations suggest that rapamycin inhibits MCM 6 and MCM 7 expression by blocking their E2F dependent transactivation which may contribute importantly to the inhibition of VSMC DNA synthesis by rapamycin . . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mitogen induced MCM 6 and MCM 7 mRNA expression was potently inhibited by nTZDpa and to a lesser degree by the full PPARgamma agonist , rosiglitazone . ^^^ Inhibition of MCM 6 and MCM 7 expression by nTZDpa and rosiglitazone paralleled their effect to inhibit phosphorylation of the retinoblastoma protein and cell proliferation . ^^^ Transient transfection experiments revealed that the nTZDpa inhibited mitogen induced MCM 6 and MCM 7 promoter activity , implicating a transcriptional mechanism . ^^^ Adenoviral mediated E2F overexpression reversed the suppressive effect of nTZDpa on MCM 6 and MCM 7 expression . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| The MCM 8 protein from HeLa cells , a new member of the MCM family , co isolates through several steps with MCM 6 and MCM 7 , and MCM 8 co immunoprecipitates with MCM 4 , MCM 6 and MCM 7 , proteins reportedly forming a helicase complex involved in initiation of DNA replication . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Using mutational analysis in the Walker A motif of MCM 6 and MCM 7 of MCM 2 7 , we show that ATP binding and / or hydrolysis by MCM proteins is dispensable for chromatin loading and pre replicative complex ( pre RC ) assembly , but is required for origin unwinding during DNA replication . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| An emerging class of markers , the minichromosome maintenance protein family of DNA licensing factors ( MCM 2 , MCM 6 , MCM 7 ) , shows promise for the specific detection of high grade cervical disease using simple antibody based immunochemistry formats . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| The present studies were designed to investigate the signal transduction pathways controlling the expression of MCM 6 and MCM 7 in VSMC in response to mitogenic stimuli . ^^^ MCM 6 and MCM 7 expression was substantially increased after stimulation with platelet derived growth factor BB and insulin . ^^^ Pretreatment with PD 98059 , a specific inhibitor of the extracellular signal regulated kinases ( ERK ) mitogen activated protein kinase ( MAPK ) , competely inhibited the mitogen induced MCM 6 and MCM 7 mRNA and protein expression , demonstrating a critical role for this pathway in transmitting transmembrane signals required for the initiation of DNA replication . ^^^ The p38MAPK inhibitor SB 203580 , the phosphatidylinositol 3 kinase ( PI 3 kinase ) pathway inhibitor wortmannin , and the protein kinase C pathway ( PKC ) inhibitor G 6976 did not significantly affect mitogen induced MCM 6 and MCM 7 expression . ^^^ Transient transfection experiments revealed that PD 98059 inhibited mitogen induced MCM 6 and MCM 7 transcriptional activation . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| In ATC , high MCM 5 and MCM 7 expression was paralleled by high levels of MCM 2 and MCM 6 . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Here , we report that Notch mediated cell cycle arrest is associated with downregulation of MCM 2 and MCM 6 in endothelial cells and human fibroblasts . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| Here , we show that expression levels of at least three of the PfMCM subunits , the homologues of MCM 2 , MCM 6 and MCM 7 , change during the intraerythrocytic development cycle , peaking in schizont and decreasing in the ring and trophozoite stages . ^^^ |
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| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q14566 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
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