| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : The Xenopus MCM complex contains homologues of yeast MCM 2 , MCM 3 , MCM 5 and Cdc 21 proteins . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| In this work , we show that Mcm 2 , Mcm 3 , and Mcm 5 self interact and interact with one another to form complexes . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| In this study , we have cloned E . histolytica ( Eh ) MCM 2 and Eh MCM 5 genes , while Eh MCM 3 was cloned earlier . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.73091687 |
| Finally , using protein protein cross linking we have shown that MCM 2 interacts directly with MCM 5 and MCM 6 ; MCM 5 with MCM 3 and MCM 2 ; and MCM 6 with MCM 2 and MCM 4 . 0.73091687^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| These include the Saccharomyces cerevisiae MCM 2 and MCM 3 proteins , which are needed for the efficient function of certain replication origins , and S . cerevisiae CDC 46 , which is required for the initiation of chromosome replication . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 2 shows striking homology to Mcm 3 and three other proteins , Cdc 46 of S . cerevisiae , and Nda 4 and Cdc 21 of Schizosaccharomyces pombe . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mutations in the cell division cycle genes CDC 46 and CDC 47 were originally isolated as suppressors of mutations in two other cell division cycle genes ( CDC 45 and CDC 54 ) . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Many of the Sup / Ts mutations exhibited a cell division cycle terminal phenotype at the high temperature , and they defined two new cdc genes ( CDC 46 and CDC 47 ) . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that some proteins containing a DEAD box like motif as well as mammalian homologues of yeast MCM 2 , MCM 3 and CDC 46 play an important role in cell free DNA replication of Xenopus eggs . . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| From a human fetal lung cDNA library , we isolated a gene , human MCM 2 , whose nucleotide sequence predicts a protein product homologous to the yeast nuclear proteins MCM 2 , MCM 3 , CDC 21 , and CDC 46 . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| The predicted protein sequences of nda1+ and nda4+ isolated by complementation are similar to each other and also , respectively , to those of the budding yeast , MCM 2 and CDC 46 , both of which are members of the gene family required for the initiation of DNA replication . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| YBR 1441 encodes a polypeptide of 845 amino acids which shares a long consensus domain with products of S . cerevisiae MCM 2 , MCM 3 , CDC 46 and Schizosaccharomyces pombe cdc21+ genes . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| CDC 45 genetically interacts with at least two members of the MCM ( minichromosome maintenance ) family of replication genes , CDC 46 and CDC 47 , which are proposed to perform a role in restricting initiation of DNA replication to once per cell cycle . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Although CDC 45 genetically interacts with a group of MCM genes ( CDC 46 , CDC 47 , and CDC 54 ) , the predicted sequence of its protein product reveals no significant sequence similarity to any known Mcm family member . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The members of the MCM protein family , including MCM 2 , MCM 3 , Cdc 21 , CDC 46 , Mis 5 and CDC 47 , are considered to be involved in the control of a single round of DNA replication during S phase in eukaryotes . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| CDC 45 interacts genetically with CDC 46 and CDC 47 , both members of the MCM family of genes which have been implicated in the licensing of DNA replication . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| This element , which we call an ECB ( early cell cycle box ) , was first identified in the SWI 4 promoter , but it is also present in the promoter of a G 1 cyclin CLN 3 , as well as in the promoters of three DNA replication genes : CDC 6 , CDC 47 , and CDC 46 . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| We characterize two mutations in CDC 47 and CDC 46 which arrest cells with unduplicated DNA as a result of single base substitutions . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| We compared the effects of disruptions of cdc 19 ( + ) ( mcm 2 ( + ) ) , cdc 21 ( + ) ( mcm 4 ( + ) ) , nda 4 ( + ) ( mcm 5 ( + ) ) and mis 5 ( + ) ( mcm 6 ( + ) ) ; in all cases , the null mutants underwent delayed S phase but were unable to proceed through the cell cycle . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting results demonstrate that mouse homologues of Mcm 2 , Mcm 5 , and Cdc 6 are displaced from chromatin in quiescent cells . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Formalin fixed paraffin embedded tissue sections from biopsy series and resections were immunostained using antibodies to Mcm 2 , Mcm 5 , and Ki 67 . ^^^ Percentage of nuclei positive for Mcm 2 , Mcm 5 , and Ki 67 was estimated and scored from 0 to 6 as : 0 , none + ; 1 , < 10 % + ; 2 , 10 30 % + ; 3 , 30 70 % + ; 4 , 70 90 % + ; 5 , > 90 % + ; 6 , all+ . ^^^ RESULTS : In non dysplastic squamous epithelium and Barrett ' s mucosa , high level expression of Mcm 2 , Mcm 5 , and Ki 67 proteins was largely confined to the proliferative compartments and downregulated in differentiated compartments . ^^^ CONCLUSIONS : Persistent expression of Mcm 2 , Mcm 5 , and Ki 67 proteins in luminal compartments of dysplastic oesophageal squamous epithelium and dysplastic Barrett ' s mucosa may be diagnostic markers and imply disruption of cell cycle control and differentiation in these dysplastic epithelia . . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 3 10 contains a P118L substitution that compromises its interaction with Mcm 5 and the recruitment of Mcm 3 and Mcm 7 to a replication origin . ^^^ P 118 is conserved between Mcm 3 , Mcm 4 , Mcm 5 , and Mcm 7 . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Cdc 23 shows genetic interactions with four MCM subunits : cdc 23 M36 and cdc 23 1E2 alleles both show synthetic phenotypes with mcm 2 ( cdc 19 P1 ) and mcm 6 ( mis 5 268 ) , and cdc 23 M36 is synthetically lethal with mcm 4 ( cdc 21 K46 ) and with mcm 5 ( nda 4 108 ) . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Moreover , cDNA array analysis showed that PC SPES caused up regulation of p 21 ( WAF1 / CIP1 ) and decreased expression of cyclin B , Nedd 8 , cdc 2 , skp 1 , PCNA , MAD2L1 , cyclin H , CKS 2 , E2F , Rbx 1 , MCM 2 , MCM 5 , Mpp 2 , Cullin Cul4A , Cks1p9 and McM 7 , which are involved in cell cycle progression . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Indeed , Mcm 7 stimulates Mcm 1 binding to the early cell cycle box upstream of the promoters of MCM 7 as well as CDC 6 and MCM 5 . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| Furthermore , depletion of Mcm 10 , Cdc 45 , Mcm 2 , Mcm 5 , and Orc 2 , respectively , results in aberrant chromosome condensation . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| In ATC , high MCM 5 and MCM 7 expression was paralleled by high levels of MCM 2 and MCM 6 . ^^^ OBJECTIVE : In an attempt to cast light on the mechanisms governing ATC , we evaluated MCM 5 and MCM 7 expression in human normal , papillary ( PTC ) , and anaplastic thyroid samples , as well as in primary culture cells and transgenic mouse models . ^^^ RESULTS : MCM 5 and MCM 7 expression was high in 65 % of ATC and negligible in normal thyroid tissue and papillary thyroid carcinomas . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| We analyzed immunohistochemically the expression of MCM 2 and MCM 5 in 65 patients with detrusor muscle invasive urothelial bladder carcinomas in relation with clinicopathologic parameters , patients ' overall and disease free survival , and the expression of the conventional proliferation index Ki 67 and other cell cycle modulators ( p 53 , pRb , p 21 ( WAF 1 ) , and p 27 ( Kip 1 ) ) . ^^^ The levels of MCM 2 and MCM 5 were significantly higher in high grade ( P < . 0001 ) , advanced stage ( P = . 001 ) , and nonpapillary tumors ( P < . 0001 ) . ^^^ The expression of MCM 2 and MCM 5 significantly associated with the conventional proliferation index Ki 67 ( P = . 0001 for each protein ) . ^^^ The expression of MCM 2 or MCM 5 positively correlated with p 53 labeling index ( P = . 014 and P = . 009 , respectively ) . ^^^ Finally , both MCM 2 and MCM 5 associated significantly with adverse patients ' outcome in both univariate ( P = . 0072 and P = . 0074 , respectively ) and multivariate ( P = . 0001 ) analysis . ^^^ |
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| Interacting proteins: P33992 and P33993 |
Pubmed |
SVM Score :0.0 |
| RESULTS : cDNA array analysis showed that cyclin B 1 , MCM 5 , MCM 7 , RAD 9 , ubiquitin C , CDK 6 , SKP 2 , and APAF 1 were up regulated in malignant thyroid neoplasms . ^^^ Real time quantitative PCR showed that MCM 5 , MCM 7 , and RAD 9 mRNA expression were significantly higher in malignant than in benign thyroid neoplasms ( < or = 0 . 0012 ) . ^^^ The combined use of MCM 5 , MCM 7 , and RAD 9 mRNA expression had a sensitivity of 98 . 2 % and a specificity of 65 . 7 % . ^^^ CONCLUSIONS : MCM 5 , MCM 7 , and RAD 9 are overexpressed in malignant thyroid neoplasms of follicular cell origin . ^^^ |
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