| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.57650314 |
| Protein hCdc 21 forms a stable trimeric complex with two novel human Mcm proteins , p85Mcm and p105Mcm . 0.57650314^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| The cdc 21 mutant is defective in the mitotic maintenance of some plasmids , like mcm 2 and mcm 3 . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 2 shows striking homology to Mcm 3 and three other proteins , Cdc 46 of S . cerevisiae , and Nda 4 and Cdc 21 of Schizosaccharomyces pombe . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| From a human fetal lung cDNA library , we isolated a gene , human MCM 2 , whose nucleotide sequence predicts a protein product homologous to the yeast nuclear proteins MCM 2 , MCM 3 , CDC 21 , and CDC 46 . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : The Xenopus MCM complex contains homologues of yeast MCM 2 , MCM 3 , MCM 5 and Cdc 21 proteins . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| We detected in HeLa cell extracts six proteins , CDC 47 , CDC46 / MCM5 , Cdc 21 , P1 / MCM3 , Mis 5 , and BM28 / MCM2 , by their binding to a specific antibody and by partial sequencing . ^^^ The human homologs of the MCM 2 ( BM 28 ) , Mis 5 , Cdc 21 , and CDC 47 proteins were tightly bound to a histone Sepharose column and purified to near homogeneity , whereas the P1 / MCM3 and CDC46 / MCM5 proteins passed through . ^^^ Among the four core histones , the human BM28 / MCM2 , Mis 5 , Cdc 21 , and CDC 47 proteins had high affinity for histone H 3 . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| This gene family includes Saccharomyces cerevisiae MCM 2 , MCM 3 , MCM5 / CDC46 and CDC 47 , S . pombe nda 1 , nda 4 , cdc 21 and mis 5 , and genes encoding human BM 28 , P1MCM3 and P1 . 1MCM3 and mouse P1MCM3 , most of which are considered to be required for the initiation of DNA replication . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The members of the MCM protein family , including MCM 2 , MCM 3 , Cdc 21 , CDC 46 , Mis 5 and CDC 47 , are considered to be involved in the control of a single round of DNA replication during S phase in eukaryotes . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Our group purified MCM protein complexes consisting of MCM 2 , 4 ( Cdc 21 ) , 6 ( Mis 5 ) , and 7 ( CDC 47 ) proteins from HeLa cells by using histone Sepharose column chromatography ( Ishimi , Y . , Ichinose , S . , Omori , A . , Sato K . , and Kimura , H . ( 1996 ) J . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| We compared the effects of disruptions of cdc 19 ( + ) ( mcm 2 ( + ) ) , cdc 21 ( + ) ( mcm 4 ( + ) ) , nda 4 ( + ) ( mcm 5 ( + ) ) and mis 5 ( + ) ( mcm 6 ( + ) ) ; in all cases , the null mutants underwent delayed S phase but were unable to proceed through the cell cycle . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mutations in the cell division cycle genes CDC 46 and CDC 47 were originally isolated as suppressors of mutations in two other cell division cycle genes ( CDC 45 and CDC 54 ) . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Although CDC 45 genetically interacts with a group of MCM genes ( CDC 46 , CDC 47 , and CDC 54 ) , the predicted sequence of its protein product reveals no significant sequence similarity to any known Mcm family member . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| The complex dissociates at high ionic strength giving rise to stable subcomplexes , one of which contains protein p85Mcm together with Mcm proteins hCdc 21 and p1O5Mcm . . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| We have also shown that mouse MCM 2 can inhibit the DNA helicase activity of the human MCM 4 , 6 , and 7 protein complex . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Furthermore , when cdc 2 kinase was inactivated in temperature sensitive mutant murine FT 210 cells , the G2 / M hyperphosphorylation of the murine MCM 2 and MCM 4 and release of the MCMs from chromatin in the G 2 phase were severely impaired . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Comparative analysis of the chromatin distribution of MCM 2 , 3 , and 4 shows that the binding of MCM 4 is distinct from that of MCM 2 and 3 . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| In addition , cdc 24 ( + ) genetically interacts with the gene encoding the catalytic subunit of DNA polymerase epsilon , which is stimulated by PCNA and RFC , and with those encoding the fission yeast counterparts of Mcm 2 , Mcm 4 , and Mcm 10 . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| In this study , we have expressed and isolated the mouse Mcm 2 , 4 , 6 , 7 proteins from insect cells and characterized various mutant Mcm 4 , 6 , 7 complexes in which the conserved ATPase motifs of the Mcm 4 and Mcm 6 proteins were mutated . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Following exit from mitosis , the Mcm 4 subunit of the cytosolic interphase complex undergoes dephosphorylation , and the Mcm 2 , Mcm 3 , or Mcm 6 subunits are then actively phosphorylated by kinase ( s ) other than cyclin dependent kinases ( Cdks ) or Cdc 7 . ^^^ Once the Mcm complex is assembled on the chromatin the Mcm 4 and the Mcm 2 proteins are the only subunits phosphorylated during the activation of the pre Rcs . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| The formation of these nucleoprotein complexes was erased by Mcm 2 that is a potential inhibitor of the Mcm 4 , 6 , 7 helicase . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 2 , a member of the Mcm 2 7 protein family essential for the initiation of DNA replication , has several biochemical activities including the ability to inhibit the Mcm 4 , 6 , 7 helicase . ^^^ It was found that Mcm 2 deleted at an amino terminal portion is able to bind to an Mcm 4 , 6 , 7 hexameric complex and to inhibit its DNA helicase activity . ^^^ The same deletion mutant of Mcm 2 and the carboxyl terminal half of Mcm 2 were both able to bind to Mcm 4 , suggesting that the carboxyl half of Mcm 2 binds to Mcm 4 to disassemble the Mcm 4 , 6 , 7 hexamer . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| It is likely that pre RCs contain heteromeric complexes of MCM 2 7 proteins , since degradation of Mcm 2 , 3 , 6 , or 7 during G 1 phase , after pre RC formation , causes loss of Mcm 4 from the nucleus . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Finally , using protein protein cross linking we have shown that MCM 2 interacts directly with MCM 5 and MCM 6 ; MCM 5 with MCM 3 and MCM 2 ; and MCM 6 with MCM 2 and MCM 4 . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| P 118 is conserved between Mcm 3 , Mcm 4 , Mcm 5 , and Mcm 7 . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Cdc 23 shows genetic interactions with four MCM subunits : cdc 23 M36 and cdc 23 1E2 alleles both show synthetic phenotypes with mcm 2 ( cdc 19 P1 ) and mcm 6 ( mis 5 268 ) , and cdc 23 M36 is synthetically lethal with mcm 4 ( cdc 21 K46 ) and with mcm 5 ( nda 4 108 ) . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Roles of Mcm 7 and Mcm 4 subunits in the DNA helicase activity of the mouse Mcm4 / 6 / 7 complex . ^^^ Biol . 19 , 8003 8015 ) , our data indicate distinct roles of Mcm 4 , Mcm 6 , and Mcm 7 subunits in activation of the DNA helicase activity of the Mcm4 / 6 / 7 complex . . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Consistently , Mcm 2 mRNA was enriched in HeLa cells to approximately four times the level in WI 38 cells , and the synthesis of Mcm 4 , 6 and 7 proteins was accelerated in HeLa cells . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| The MCM 8 protein from HeLa cells , a new member of the MCM family , co isolates through several steps with MCM 6 and MCM 7 , and MCM 8 co immunoprecipitates with MCM 4 , MCM 6 and MCM 7 , proteins reportedly forming a helicase complex involved in initiation of DNA replication . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Such cells show a specific defect in loading of initiator proteins Mcm 4 , Mcm 7 , and to a lesser degree , Mcm 2 onto chromatin during telophase and early G 1 when Mcm 2 7 are normally recruited to license origins of replication . ^^^ Because minichromosome maintenance complex proteins are thought to function as a heterohexamer , loading of Mcm 2 , Mcm 4 , and Mcm 7 depleted complexes is likely to underlie the S phase defects observed in cyclin E deregulated cells , consistent with a role for minichromosome maintenance complex proteins in initiation of replication and fork movement . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| Consistent with the DNA replication defects , chromatin immunoprecipitation experiments revealed significantly lower levels of the replication factors Mcm 4 , Mcm 7 , and Cdc 45 at replication origins in met 30 mutants than in wild type cells . ^^^ |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P33991 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|