| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.52416903 |
| Finally , using protein protein cross linking we have shown that MCM 2 interacts directly with MCM 5 and MCM 6 ; MCM 5 with MCM 3 and MCM 2 ; and MCM 6 with MCM 2 and MCM 4 . 0.52416903^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : The Xenopus MCM complex contains homologues of yeast MCM 2 , MCM 3 , MCM 5 and Cdc 21 proteins . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| In this study , we have cloned E . histolytica ( Eh ) MCM 2 and Eh MCM 5 genes , while Eh MCM 3 was cloned earlier . ^^^ |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| All of these phenotypes are precisely those exhibited by mutants in three MCM genes , MCM 2 , MCM 3 , and MCM5 / CDC46 , that have been shown to play interacting roles in the early steps of DNA replication . . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| These include the Saccharomyces cerevisiae MCM 2 and MCM 3 proteins , which are needed for the efficient function of certain replication origins , and S . cerevisiae CDC 46 , which is required for the initiation of chromosome replication . ^^^ The cdc 21 mutant is defective in the mitotic maintenance of some plasmids , like mcm 2 and mcm 3 . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 2 and Mcm 3 , two proteins important for ARS activity , are related in structure and function . ^^^ MCM 2 and MCM 3 are essential genes believed to play important roles in the initiation of DNA replication in Saccharomyces cerevisiae . ^^^ Mutants defective in Mcm 2 or Mcm 3 are remarkably similar in phenotype . ^^^ Double mutants of mcm 2 1 and mcm 3 1 are inviable at the permissive growth temperature ( 23 degrees C ) for each of the single mutants . ^^^ Furthermore , overproduction of Mcm 3 accentuates the deleterious effect of the mcm 2 1 mutation , whereas overproduction of Mcm 2 partially complements the mcm 3 1 mutation . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| The phenotype exhibited by the mcm 3 mutant is very similar to that of mcm 2 , suggesting that the Mcm 2 and Mcm 3 protein may play interacting roles in DNA replication . . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| The yeast MCM 2 , MCM 3 , and MCM5 / CDC46 genes are required for DNA replication and have been proposed to act as factors that license the DNA for one and only one round of replication per cell cycle . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that some proteins containing a DEAD box like motif as well as mammalian homologues of yeast MCM 2 , MCM 3 and CDC 46 play an important role in cell free DNA replication of Xenopus eggs . . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| From a human fetal lung cDNA library , we isolated a gene , human MCM 2 , whose nucleotide sequence predicts a protein product homologous to the yeast nuclear proteins MCM 2 , MCM 3 , CDC 21 , and CDC 46 . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Cell cycle regulated nuclear localization of MCM 2 and MCM 3 , which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast . ^^^ MCM 2 and MCM 3 are two genetically interacting and structurally related proteins essential for growth in Saccharomyces cerevisiae . ^^^ We show further that the nuclear and subnuclear localizations of the MCM 2 and MCM 3 proteins are temporally regulated with respect to the cell cycle . ^^^ Once inside the nucleus , a fraction of the MCM 2 and MCM 3 proteins becomes tightly associated with DNA . ^^^ The association of MCM 2 and MCM 3 with chromatin presumably leads to the initiation of DNA synthesis , and their subsequent disappearance from the nucleus presumably prevents reinitiation of DNA synthesis at replication origins . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| YBR 1441 encodes a polypeptide of 845 amino acids which shares a long consensus domain with products of S . cerevisiae MCM 2 , MCM 3 , CDC 46 and Schizosaccharomyces pombe cdc21+ genes . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| These strains possess temperature sensitive ( ts ) mutations designated mcm 2 and mcm 3 , respectively . ^^^ Because W . dermatitidis either lacks or has an undiscovered sexual cycle , parasexual methods of analysis were used to confirm that mcm 2 and mcm 3 define separate bud emergence control genes in the wild type strain . ^^^ These results imply that yeast development in the fusion products resulted from intergenic complementation of mcm 2 and mcm 3 , i . e . , they are nonallelic . ^^^ Because mcm 2 and mcm 3 are equivalent to certain cdc lesions in the yeast Saccharomyces cerevisiae , we have renamed the analogous genes defined by the mutations in W . dermatitidis as CDC 1 and CDC 2 . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 2 , Mcm 3 , and Mcm5 / Cdc46 are conserved proteins essential for the initiation of DNA synthesis at replication origins in Saccharomyces cerevisiae . ^^^ In this work , we show that Mcm 2 , Mcm 3 , and Mcm 5 self interact and interact with one another to form complexes . ^^^ Mcm 2 and Mcm 3 are abundant proteins , present in approximately 4 10 10 ( 4 ) and 2 10 10 ( 5 ) copies per cell , respectively . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| This gene family includes Saccharomyces cerevisiae MCM 2 , MCM 3 , MCM5 / CDC46 and CDC 47 , S . pombe nda 1 , nda 4 , cdc 21 and mis 5 , and genes encoding human BM 28 , P1MCM3 and P1 . 1MCM3 and mouse P1MCM3 , most of which are considered to be required for the initiation of DNA replication . ^^^ |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The members of the MCM protein family , including MCM 2 , MCM 3 , Cdc 21 , CDC 46 , Mis 5 and CDC 47 , are considered to be involved in the control of a single round of DNA replication during S phase in eukaryotes . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| The wild type huCdc 7 protein expressed in COS 7 cells phosphorylates MCM 2 and MCM 3 proteins in vitro , suggesting that huCdc 7 may regulate processes of DNA replication by modulating MCM functions . . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 2 and Mcm 3 are constitutive nuclear proteins that exhibit distinct isoforms and bind chromatin during specific cell cycle stages of Saccharomyces cerevisiae . ^^^ Our studies indicate that only a small fraction of Mcm 2 and Mcm 3 tightly associates with chromatin , from late M phase to the beginning of the S phase . ^^^ The rest of the Mcm 2 and Mcm 3 proteins are disturbed to both the cytoplasm and nucleoplasm in relatively constant levels throughout the cell cycle . ^^^ We also show that S . cerevisiae Mcm 3 is a phosphoprotein that exists in multiple isoforms and that distinct isoforms of Mcm 2 and Mcm 3 can be detected at specific stages of the cell cycle . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| DNA cloning and expression during development of Drosophila melanogaster MCM 3 , MCM 6 and MCM 7 . cDNAs encoding three Drosophila melanogaster MCM proteins , DmMCM 3 , DmMCM 6 and DmMCM 7 , candidates of DNA replication licensing factors , were cloned and sequenced . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Following exit from mitosis , the Mcm 4 subunit of the cytosolic interphase complex undergoes dephosphorylation , and the Mcm 2 , Mcm 3 , or Mcm 6 subunits are then actively phosphorylated by kinase ( s ) other than cyclin dependent kinases ( Cdks ) or Cdc 7 . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| The viral IE 86 protein induced a significant increase in the cellular steady state RNA level from the B myb , cyclin E , cdk 2 , E2F 1 , ribonucleotide reductase 1 , ribonucleotide reductase 2 , thymidylate synthetase , MCM 3 , and MCM 7 genes , but actin RNA was not affected . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Mcm 3 10 contains a P118L substitution that compromises its interaction with Mcm 5 and the recruitment of Mcm 3 and Mcm 7 to a replication origin . ^^^ P 118 is conserved between Mcm 3 , Mcm 4 , Mcm 5 , and Mcm 7 . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| MCM 7 and MCM 3 were identified as cyclin D 1 binding proteins . ^^^ Catalytically active cyclin D1 / CDK4 complexes were incorporated into chromatin bound protein complexes with the same kinetics as MCM 7 and MCM 3 , where they associated specifically with MCM 7 . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Expression of replication licensing factors MCM 2 and MCM 3 in normal , hyperplastic , and carcinomatous endometrium : correlation with expression of Ki 67 and estrogen and progesterone receptors . ^^^ The aim of the present study was to investigate the clinicopathologic implications of the MCM 2 and MCM 3 in endometrial carcinogenesis . ^^^ The authors investigated the immunohistochemical expression of MCM 2 and MCM 3 , Ki 67 , estrogen receptor , and progesterone receptor in 23 normal endometria , 9 endometrial hyperplasias , and 60 endometrial carcinomas . ^^^ In the normal endometrial glands , the expression of MCM 2 and MCM 3 was significantly higher in the proliferative phase than in the secretory phase and was strongly correlated with Ki 67 expression . ^^^ In endometrial carcinomas , however , the expression of MCM 2 and MCM 3 was significantly lower than that in the normal proliferative endometrium . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Employing this protocol , analyses of chromatin bound MCM 2 , MCM 3 , Cdc 6 and cdk 2 suggests that the ' classical ' prereplication complex is already formed during hypoxia . . ^^^ |
|
| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Genetic experiments showed that the two hybrid isolates MGS 1 , CLF 1 , MCM 3 , 5 , 7 ( CDC 47 ) , and YDL089w , when overexpressed , partially suppress the csm1Delta / csm1Delta sporulation defect . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| These include genes encoding the anti apoptosis factor SURVIVIN , positive cell cycle regulators ( CDC 2 , CYCLIN B 1 , CYCLIN B 2 , CYCLIN G 1 , CHK 1 , BUB 3 , STK 6 , SKB 1 , CSE 1 L ) and chromosome replication proteins ( MCM 2 , MCM 3 , FEN 1 , RRM 2 , TOP2A , RFC 1 ) . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Here we identify two partial nuclear localization signals ( NLSs ) on Mcm 2 and Mcm 3 that are each necessary , but not sufficient , for nuclear localization of the Mcm 2 7 complex . ^^^ Remarkably , the Mcm 2 Mcm3 NLS and the Mcm 3 NES are sufficient to form a transport module that recapitulates the cell cycle regulated localization of the entire Mcm 2 7 complex . ^^^ Moreover , we show that CDK regulation promotes net export by phosphorylation of the Mcm 3 portion of this module and that nuclear export of the Mcm 2 7 complex is sufficient to disrupt replication initiation . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| RESULTS : MCM 2 and MCM 3 proteins that were associated with the chromatin / matrix fraction in G 1 phase of both uninfected and SV 40 infected cells were gradually released during progression through S phase . ^^^ However , in SV 40 infected cells that progressed beyond DI = 2 . 0 , chromatin / matrix associated MCM 2 and MCM 3 remained at the low levels observed at the end of S phase . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| Some of the down regulated genes are involved in : cytoskeleton and cell movement ( WASF 2 , LCP 1 , MSN , NIPSNAP 1 , KIF2C ) ; DNA replication and repair ( MCM 2 , MCM 3 , MCM 7 and XRCC 4 ) . ^^^ |
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| Interacting proteins: P25205 and P33993 |
Pubmed |
SVM Score :0.0 |
| The binding of the prereplication complex proteins Orc 1 , Orc 2 , Mcm 3 , Mcm 7 , and Cdc 6 and the novel DNA unwinding element ( DUE ) binding protein DUE B to the endogenous human c myc replicator was studied by chromatin immunoprecipitation . ^^^ In G ( 1 ) arrested HeLa cells , Mcm 3 , Mcm 7 , and DUE B were prominent near the DUE , while Orc 1 and Orc 2 were least abundant near the DUE and more abundant at flanking sites . ^^^ Similarly , the signals from Orc 1 , Mcm 3 , and Mcm 7 were at background levels in cells arrested in M phase , whereas Orc 2 retained the distribution seen in G ( 1 ) phase cells . ^^^ |
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