| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Purified baculovirus expressed HsCdc 7 HsDbf4 selectively phosphorylates the MCM 2 subunit of the minichromosome maintenance ( MCM ) protein complex isolated by immunoprecipitation with MCM 7 antibodies in vitro . ^^^ Two dimensional tryptic phosphopeptide mapping analysis of in vivo ( 32 ) P labeled MCM 2 from HeLa cells reveals that several major tryptic phosphopeptides of MCM 2 comigrate with those of MCM 2 phosphorylated by HsCdc 7 HsDbf4 in vitro , suggesting that MCM 2 is a physiological HsCdc 7 HsDbf4 substrate . ^^^ These results indicate that the HsCdc 7 HsDbf4 kinase is directly involved in regulating the initiation of DNA replication by targeting MCM 2 protein in mammalian cells . . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| The wild type huCdc 7 protein expressed in COS 7 cells phosphorylates MCM 2 and MCM 3 proteins in vitro , suggesting that huCdc 7 may regulate processes of DNA replication by modulating MCM functions . . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| ASK forms an active kinase complex with huCdc 7 that is capable of phosphorylating MCM 2 protein . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Among them , MCM 2 protein , either in a free form or in a MCM 2 4 6 7 complex , serves as an excellent substrate for huCdc 7 ASK kinase complex in vitro . ^^^ MCM 2 and 4 in the MCM 2 4 6 7 complex are phosphorylated by Cdks as well , and prior phosphorylation of the MCM 2 4 6 7 complex by Cdks facilitates phosphorylation of MCM 2 by huCdc 7 , suggesting collaboration between Cdks and Cdc 7 in phosphorylation of MCM for initiation of S phase . huCdc 7 and ASK proteins can also be phosphorylated by Cdks in vitro . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Indeed , over expression of both huCdc 7 and ASK results in the elevated phosphorylation of endogenous MCM 2 protein , as manifested by appearance of the mobility shifted form on SDS PAGE , but does not cause any significant effects on cell cycle progression . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Drf1 / ASKL1 can bind and activate huCdc 7 , and Cdc 7 ASKL1 complex phosphorylates MCM 2 . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Following exit from mitosis , the Mcm 4 subunit of the cytosolic interphase complex undergoes dephosphorylation , and the Mcm 2 , Mcm 3 , or Mcm 6 subunits are then actively phosphorylated by kinase ( s ) other than cyclin dependent kinases ( Cdks ) or Cdc 7 . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of Mcm 2 , 4 , 6 , 7 complexes with Cdc 7 kinase showed that the amino terminal region of Mcm 2 is required for the phosphorylation , and it contains major Cdc 7 mediated phosphorylation sites . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| These procedures identified > 60 MGMT interacting proteins with diverse functions including those involved in DNA replication and repair ( MCM 2 , PCNA , ORC 1 , DNA polymerase delta , MSH 2 , and DNA dependent protein kinase ) , cell cycle progression ( CDK 1 , cyclin B , CDK 2 , CDC 7 , CDC 10 , 14 3 3 protein , and p 21 ( waf1 / cip1 ) ) , RNA processing and translation ( poly ( A ) binding protein , nucleolin , heterogeneous nuclear ribonucleoproteins , A2 / B1 , and elongation factor 1alpha ) , several histones ( H 4 , H3 . 4 , and H2A . 1 ) , and topoisomerase 1 . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Cdc 7 , Cdk 2 , and ataxia telangiectasia and Rad 3 related kinases regulate S phase entry and S phase progression and are known to phosphorylate the Mcm 2 subunit . ^^^ In this work , by in vitro kinase reactions and mass spectrometry analysis of the products , we have mapped phosphorylation sites in the N terminus of Mcm 2 by Cdc 7 , Cdk 2 , Cdk 1 , and CK 2 . ^^^ We found that Cdc 7 phosphorylates Mcm 2 in at least three different sites , one of which corresponds to a site also reported to be phosphorylated by ataxia telangiectasia and Rad 3 related . ^^^ Importantly , although all the Cdc 7 dependent Mcm 2 phosphosites fluctuate during the cell cycle with kinetics similar to Cdc 7 kinase activity and Cdc 7 protein levels , phosphorylation of Mcm 2 in the putative cyclin dependent kinase ( Cdk ) consensus sites is constant during the cell cycle . ^^^ Furthermore , our analysis indicates that the majority of the Mcm 2 isoforms phosphorylated by Cdc 7 are not stably associated with chromatin . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| In this study , we have mapped the sites in human Mcm 2 protein that are phosphorylated by Cdc 7 . ^^^ Alanine substitution experiments with Mcm 2 peptides showed that the phosphorylation of ( 5 ) S and ( 53 ) S by Cdc 7 required the presence of an acidic amino acid adjacent to a serine residue . ^^^ Furthermore , although Cdc 7 was unable to phosphorylate a Mcm 2 peptide ( spanning amino acids 19 30 and containing ( 26 ) S and ( 27 ) S ) , it phosphorylated ( 26 ) S efficiently when this peptide contained a chemically synthesized phospho ( 27 ) S modification . ^^^ Hence , additional Cdc 7 phosphorylation sites could be generated in Mcm 2 by its prior phosphorylation by a cyclin dependent kinase . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Purified Hsk 1 phosphorylates the Cdc 19 ( Mcm 2 ) subunit of the six member minichromosome maintenance protein complex purified from fission yeast . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Him 1 , identical to Dfp 1 , previously identified as an associated molecule of Hsk 1 , binds to Hsk 1 and stimulates its kinase activity , which phosphorylates both catalytic and regulatory subunits as well as recombinant MCM 2 protein in vitro . him 1 ( + ) is essential for DNA replication in fission yeast cells , and its transcription is cell cycle regulated , increasing at middle M to late G ( 1 ) . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| Analyses of a newly isolated temperature sensitive mutant , hsk 1 89 , reveal that Hsk 1 plays crucial roles in DNA replication checkpoint signaling and maintenance of proper chromatin structures during mitotic S phase through regulating the functions of Rad 3 ( ATM ) Cds 1 and Rad 21 ( cohesin ) , respectively , in addition to expected essential roles for initiation of mitotic DNA replication through phosphorylating Cdc 19 ( Mcm 2 ) . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| This protein binds and activates Hsk 1 kinase , which phosphorylates the MCM 2 protein . ^^^ |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00311 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
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