Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.71112434
The interactions between CD 80 and CD 86 on antigen presenting cells and CD 28 on T cells serve as an important costimulatory signal in the activation of T cells . 0.71112434^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Primary and cultured H RS cells frequently overexpress intercellular adhesion molecule 1 ( ICAM 1 / CD54 ) , BB 1 ( B 7 1 / CD80 ) and B70 / B7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The antibody BB 1 did not inhibit proliferation and cytokine secretion , nor did the anti CD 86 clone IT2 . 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To examine if the muscle fibers in patients with inflammatory myopathies have the potential to behave as antigen presenting cells ( APCs ) , we investigated the expression of costimulatory molecules BB 1 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) , and their counterreceptors , CD 28 or CTLA 4 ( CD 152 ) , in the muscle biopsies of patients with polymyositis ( PM ) , PM associated with human immunodeficiency virus infection ( HIV PM ) , sporadic inclusion body myositis ( s IBM ) , dermatomyositis ( DM ) , and normal or disease controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To examine whether the Schwann cells in patients with autoimmune neuropathies have the potential to behave as professional antigen presenting cells , we investigated the expression of the co stimulatory molecules BB 1 , B 7 1 ( CD 80 ) B 7 2 ( CD 86 ) and their counter receptors CD 28 or CTLA 4 ( CD 152 ) at the protein and mRNA levels in sural nerve biopsies of patients with chronic inflammatory demyelinating polyneuropathy ( CIDP ) , CIDP associated with human immunodeficiency virus infection ( HIV CIDP ) , IgM paraproteinaemic neuropathy and normal or non immune axonal neuropathy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We investigated expression of costimulatory molecules BB 1 , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and their counter receptors CD 28 and CTLA 4 ( CD 152 ) in muscle biopsy specimens of patients with scleroderma polymyositis overlap syndrome ( SSc PM ) , primary polymyositis ( PM ) , and other related diseases to examine whether the muscle fibers in patients with SSc PM behave as antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In particular , CD 40 triggering induced maintenance of high levels of major histocompatibility complex class 2 antigens and upregulation of accessory molecules such as CD 58 , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It is interesting to note that mAbs against CD 86 completely prevented the binding of CTLA 4 Ig in the presence of mAbs against CD 80 and bound to a B70 / B7 2 transfected fibroblast cell line , demonstrating that the B70 / B7 2 antigen is identical to CD 86 . ^^^ This inhibitory effect was enhanced to > or = 90 % when a combination of anti CD 80 and anti CD 86 mAbs was used . ^^^ The present results demonstrate that D Lc express , in addition to CD 80 , the other ligand for CTLA 4 , CD 86 ( B70 / B7 2 ) , which plays a primordial role during D Lc induced alloreaction . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 ) and CD 86 ( B 70 ) provide similar costimulatory signals for T cell proliferation , cytokine production , and generation of CTL . ^^^ Recently , it has been demonstrated that CD 28 interacts with two different ligands , designated CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) . ^^^ We have produced stable transfectants that express CD 80 , CD 86 , or both ligands and have examined their ability to costimulate T cell proliferation , cytokine production , and the generation of CTL . ^^^ When we used small , resting human peripheral blood T cells as responders , both CD 80 and CD 86 transfectants efficiently costimulated anti CD 3 mAb induced proliferation and the secretion of IL 2 and IFN gamma . ^^^ Additionally , both CD 80 and CD 86 transfectants were able to generate functional CTL . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recently , diversities between CD 80 and CD 86 in expression , regulation , and function have been reported in certain cell populations and murine experimental disease models . ^^^ To investigate the possible differential role of CD 80 and CD 86 in the development of lupus , we treated lupus prone NZB / W F 1 mice with specific monoclonal antibodies ( mAb ) against CD 80 , CD 86 , or both . ^^^ The treatment with a combination of anti CD 80 and CD 86 mAb before the onset of lupus completely prevented autoantibody production and nephritis , and prolonged survival . ^^^ Interestingly , we found that anti CD 86 mAb alone , but not anti CD 80 mAb , efficiently inhibited autoantibody production . ^^^ The incomplete reduction of IgG2a anti dsDNA antibody by anti CD 86 mAb was compensated by the addition of anti CD 80 mAb . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis of the cells during a 2 week culture revealed a loss of CD 14 and CD 34 expression , a concomittent increase of CD1a , CD11a , b and c , CD 44 , CD 45 , CD 54 , HLA class 1 and 2 , and intermediate levels of CD 26 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Finally , CD40L CD 40 interactions do not induce CD 80 , CD 86 , or major histocompatibility complex class 2 expression on HUVEC in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory molecules B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and interleukin 12 cytokine in multiple sclerosis lesions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we demonstrate that B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are also expressed on murine T cells in the absence of major histocompatibility complex class 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential modulation of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression . ^^^ Such cellular interactions are mediated by surface molecules including MHC class 2 Ags ( DR ) and CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^ Normal and psoriatic skin derived DDCs express high levels of CD 86 over CD 80 , and the overall hierarchy is DR > CD 86 > CD 80 , whereas cultured monocytes express low and equivalent levels of CD 80 and CD 86 . ^^^ If Ab is added to GM CSF at the initial period of cultivation , DDCs that emigrate have lower levels of CD 86 without any detectable effect on CD 80 or DR expression and display a reduced capacity to stimulate either superantigen driven or alloantigen responsive T cells . ^^^ When IL 10 was added at the beginning of culture , DDCs had significantly lower levels of CD 86 , without any effect on CD 80 or DR expression , and like anti GM CSF treated cells , these DDCs had approximately a 50 % reduction in their T cell stimulating capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Human B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) bind with similar avidities but distinct kinetics to CD 28 and CTLA 4 receptors . ^^^ B 7 0 or B 7 2 ( CD 86 ) is a T cell costimulatory molecule that binds the same receptors ( CD 28 and CTLA 4 ) as B 7 1 ( CD 80 ) , but shares with it only approximately 25 % sequence identity and is expressed earlier during an immune response . ^^^ Here we show that human CD 86 maintains similar ( within approximately 2 to 3 fold ) overall receptor binding and T cell costimulatory properties as CD 80 . ^^^ However , CD 80 and CD 86 did not bind equivalently to CTLA 4 : CD 80 bound Y100A , a form of CTLA4lg with a mutation in the CDR 3 like region , > 200 fold better than did CD 86 ; inhibition of CD 80 mediated cellular responses required approximately 100 fold lower CTLA4lg concentrations ; and CD 80 CTLA4lg complexes dissociated 5 to 8 fold more slowly , Thus , CD 80 and CD 86 utilize different binding determinants and have different kinetics of binding to CD 28 and CTLA 4 . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immunoglobulin fold characteristics of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD28 / CTLA 4 ligands : the gene encoding CD 86 ( B70 / B7 . 2 ) maps to the same region as CD 80 ( B7 / B7 . 1 ) gene in human chromosome 3q13 q 23 . ^^^ The CD 86 gene is , therefore , located in the proximity of the CD 80 ( B7 / B7 . 1 ) gene , the first identified ligand for CD 28 and CTLA 4 , previously mapped to chromosome 3q13 . 3 q 21 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 are homologous T cell receptors of the immunoglobulin ( Ig ) superfamily , which bind B 7 molecules ( CD 80 and CD 86 ) on antigen presenting cells and transmit important costimulatory signals during T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Engagement of the TCR / CD3 complex together with ligation of CD 28 by its counterstructures B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on APC are required for mitogenic T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
One of these residues , Y 87 , is conserved in all CD 80 and CD 86 cloned from various species . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The B 7 related molecules CD 80 and CD 86 are expressed on antigen presenting cells , bind the homologous T cell receptors CD 28 and CTLA 4 , and trigger costimulatory signals important for optimal T cell activation . ^^^ All four molecules are immunoglobulin superfamily members , each comprising an extracellular Ig variable like ( IgV ) domain , with CD 80 and CD 86 containing an additional Ig constant like ( IgC ) domain . ^^^ Despite limited sequence identity , CD 80 and CD 86 share similar overall receptor binding properties and effector functions . ^^^ We have identified , by site directed mutagenesis of soluble forms of CD 80 and CD 86 , residues in both the IgV and IgC domains that are important for CTLA4Ig and CD28Ig binding . ^^^ In general , mutations had a similar effect on CTLA4Ig and CD28Ig binding , thereby indicating that both receptors bind to overlapping sites on CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interactions between CD28 / CTLA 4 on T cells and CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) counter receptors provide crucial costimulatory signals for TCR alpha beta+ lymphocytes . ^^^ Alloreactivity of CD 28 / and CD28+ / Tg gamma delta+ thymocytes could not be blocked with mAbs against CD 80 and CD 86 ligands . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The second , or costimulatory signal , can be provided by ligation of the B lymphocyte activation antigens B 7 1 ( CD 80 ) and B7 . 2 ( CD 86 ) to TCR antigen CD 28 . ^^^ The cDNAs for both CD 80 and CD 86 have been isolated and are predicted to encode type 1 membrane proteins of the immunoglobulin ( Ig ) superfamily . ^^^ Here we report that the genomic organization of CD 86 reflects its functional structure , and is similar to that found for CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In these experiments , the expression and function of the CD28 / CTLA 4 ligands B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) were examined on human blood DC . ^^^ Resting DC populations directly isolated by immunodepletion of lineage marker positive cells lacked cell membrane expression of CD 80 and expressed little or no CD 86 , although CD 86 , but not CD 80 mRNA was detected by reverse transcription polymerase chain reaction analysis . ^^^ In contrast , low density DC isolated after culture in vitro strongly expressed CD 86 surface protein , but expressed limited or no CD 80 , although mRNA for both molecules were detected . ^^^ Short term culture of directly isolated DC up regulated both CD 80 and CD 86 expression . ^^^ Analysis of the kinetics of CD28 / CTLA 4 ligand induction showed that surface CD 86 was present within 8 h , whereas CD 80 antigen was first detected after 24 h of culture . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interleukin 10 differentially regulates B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression on human peripheral blood dendritic cells . ^^^ Flow cytometric analysis indicated that rIL 10 did not modify the expression of ICAM 1 ( CD 54 ) and B 7 1 ( CD 80 ) , but decreased HLA DR and B 7 2 ( CD 86 ) expression at the DC surface . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Approximately 2 to 8 million cells with the characteristics of dendritic cells could be obtained : phase contrast microscopy revealed the typical cytoplasmic processes or veils ; phenotypic analysis confirmed expression of dendritic cell associated molecules , including MHC class 2 , CD1a , CD 4 , ICAM 1 ( CD 54 ) , LFA 3 ( CD 58 ) , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , and absence of T cell , B cell and monocyte markers ; in addition , these cells rapidly attached to and migrated on collagen type 1 coated surfaces . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Precursor derived DC express high levels of CD 86 ( B 7 2 ) and HLA DR but no CD 80 ( B 7 1 ) , whereas mature DC have high levels of expression of all three markers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Unexpectedly , tumor cells of 5 cases expressed at least one member of the B 7 family ( CD 80 , CD 86 , and B 7 3 ) and many lymphoid cells expressing the corresponding counter receptor , CD 28 , were detected in all cases . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) induce long term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro . ^^^ In this report , the co stimulatory signals provided by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) were compared to CD 28 ligation by mAb . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both CD 28 ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) activate phosphatidylinositol 3 kinase , and wortmannin reveals heterogeneity in the regulation of T cell IL 2 secretion . ^^^ In this report , the co stimulatory signals provided by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) were compared to CD 28 ligation by mAb . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , the levels of CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) antigen expression were significantly higher on these T cells and alveolar macrophages in the BALF of patients . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Production of the various cytokine mRNAs following alloantigen stimulation was inhibited by CTLA4Ig and thus remained under the regulation of CD 80 and CD 86 expressed on the EBV LCL . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
All three clone types were able to activate B cells as shown by up regulation of CD 25 , CD 80 and CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) : potential targets for immunotherapy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recognition of antigen / MHC complexes by the T cell receptor delivers the first signal , while a second signal , delivered by the cell surface receptors CD 80 and / or CD 86 binding to the T cell surface molecule CD 28 , has been shown to be effective for the initiation of effective T cell responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Distinct roles for B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in the initiation of experimental allergic encephalomyelitis . ^^^ The present studies examined the role of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulation in the prototypic autoimmune disorder , experimental allergic encephalomyelitis ( EAE ) . ^^^ In adoptively transferred EAE , in vitro activation of myelin basic protein ( MBP ) specific lymph node cells was inhibited by the combination of anti CD 80 plus anti CD 86 , but not individually . ^^^ However , in actively induced disease , one injection of anti CD 80 significantly reduced disease , while anti CD 86 exacerbated disease . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Among various molecules known to provide costimulatory function , the expression of CD 54 ( ICAM 1 ) and CD11a / CD18 ( LFA 1 ) on MAIDS B cells was increased , whereas that of CD 2 , heat stable Ag ( CD 24 ) , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) was unchanged from normal . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A major co stimulatory pathway involves crosslinking the CD 28 molecule on T cells by its ligands CD 80 or CD 86 expressed on antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , IL 10 led to the down regulation of various surface antigens , especially of CD 86 and CD 54 , whereas TNF alpha and TRAP enhanced the expression of MHC class 1 and 2 antigens and of the accessory molecules CD 40 , CD 54 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Functional analysis of CD83+ cells revealed that MHC class 2 , CD11a , CD 40 and CD 86 played functionally dominant roles , whereas CD 80 contributed minimally to the specialized costimulatory activity of these potent APC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Highly purified cell preparations of mLC migrating from human epidermis expressed high amounts of major histocompatibility complex ( MHC ) class 1 and 2 antigens and of the accessory molecules CD 40 , CD 80 and CD 86 , indicative of the phenotype of potent immunostimulatory cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The functional maturation of dendritic cells ( DC ) and other antigen presenting cells is believed to reflect the upregulation of cell surface major histocompatibility complex ( MHC ) class 2 and other T cell co stimulatory molecules , especially the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
As GC cells , these cells also expressed CD 71 , CD 80 ( B7 . 1 ) , and CD 86 ( B7 . 2 ) , but not CD 24 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential effects of CTLA 4 substitutions on the binding of human CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ CTLA 4 expressed on activated T cells binds to CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules present on APC with high avidity and appears to deliver a negative regulatory signal to the T cell . ^^^ We have investigated the kinetics of CTLA 4 binding to CD 80 and CD 86 , together with the effects of selected CTLA 4 mutations on binding activity . ^^^ The dissociation constants ( Kd ) for binding of CTLA 4 Ig to CD 80 and CD 86 transfectants were 8 . 1 and 6 . 7 nM , respectively . ^^^ Surface plasmon resonance was used to determine kinetic parameters of CTLA 4 Ig binding to CD 80 Ig and CD 86 Ig fusion proteins and revealed enhanced association ( ka ) and dissociation ( kd ) rate constants for CD 86 Ig compared with CD 80 Ig . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Malignant B lymphocytes from non Hodgkin ' s lymphoma induce allogeneic proliferative and cytotoxic T cell responses in primary mixed lymphocyte cultures : an important role of co stimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) in stimulation by tumor cells . ^^^ To investigate the role of co stimulatory molecules CD 80 and CD 86 for the stimulatory capacities of B cells , the expression of both molecules was analyzed before and during the MLR . ^^^ Most fresh malignant B cells were negative for CD 80 and CD 86 , whereas co cultured B cells expressed high levels of both molecules . ^^^ This expression was crucial for T cell proliferation , since monoclonal antibodies directed against CD 80 and CD 86 completely abrogated the MLR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Effect of CD 80 and CD 86 on T cell cytokine production . ^^^ Conjugation of the T cell receptor ( TCR ) with antigen / MHC proteins must be accompanied by conjugation of T cell counterreceptors ( CD 28 or CTLA 4 ) with costimulatory molecules CD 80 or CD 86 ( B 7 1 or B 7 2 ) on antigen presenting cells ( APC ) to avert T cell anergy , and to provide essential signals for T cell activation and cytokine production . ^^^ To determine the involvement of CD 80 and CD 86 in costimulation of T cell cytokine production , T cells were incubated with peritoneal exudate macrophages , which express CD 80 and CD 86 , and stimulated in vitro for 48 or 72 hrs with anti CD 3 in the presence or absence of blocking antibody to CD 80 or CD 86 . ^^^ Production of T cell IL 2 , IL 4 , and IL 5 was depressed in the presence of anti CD 86 but not anti CD 80 . ^^^ Production of IFN gamma was significantly blocked by either anti CD 80 and anti CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The results suggest that potent co stimulatory pathways can be induced by cellular interactions between a T lymphoma , RMA S and T cells , not involving gp 39 , CD 40 , CD 70 , HSA , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interactions of CD 80 and CD 86 with CD 28 and CTLA 4 . ^^^ CD 80 and CD 86 are cell surface glycoproteins expressed on a variety of professional APCs . ^^^ These results suggested that other parts of the proteins are important in determining binding , so a series of modified CD 80 and CD 86 molecules were constructed in an attempt to identify other binding determinants . ^^^ Insertion of two residues between the two Ig domains of CD 80 resulted in decreased affinity for CTLA 4 , but a similar mutation in CD 86 was without effect . ^^^ We also identified another asymmetry between CD 80 and CD 86 in that the 5 domain of CD 86 but not that of CD 80 is sufficient for CTLA 4 binding . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fluorescence activated cell sorter analysis revealed that no staining for either CD 80 or CD 86 was observed in freshly isolated Langerhans cells but that both CD 80 and CD 86 were expressed on cultured Langerhans cells . ^^^ Analysis of polymerase chain reaction demonstrated that both CD 80 and CD 86 were specifically amplified from purified cultured and freshly isolated Langerhans cells but not from Langerhans cell depleted epidermal cells , indicating that both CD 80 and CD 86 genes were expressed by Langerhans cells . ^^^ A monoclonal antibody against CD 86 caused 81 % inhibition in contrast with 29 % inhibition produced by anti CD 80 monoclonal antibody . ^^^ This inhibitory effect was enhanced to 85 . 3 % inhibition when a combination of anti CD 86 and anti CD 80 was administered . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Main costimulatory signals are provided to T cells by B 7 antigens ( CD 80 and CD 86 , expressed on antigen presenting cells ( APC ) ) upon interaction with its receptor , the CD 28 molecule expressed on T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast to macrophages , all three DC populations expressed the DC marker CD 83 , as well as highlevels of MHC molecules and the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The present study compares the mitogen activated protein ( MAP ) kinase responses in T cells activated with the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 / B70 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) co stimulatory molecules have been reported to activate Th1 / Th2 development pathways differentially . ^^^ Based on the recently reported effect of interferon ( IFN ) gamma and interleukin ( IL ) 10 on the expression of CD 80 and CD 86 by LC , we examined the effects of these cytokines on the expression of CD 54 ( intercellular adhesion molecule 1 ) and CD 40 in addition to CD 80 and CD 86 on LC , and correlated the expression of each co stimulatory molecule with antigen presentation for a Th 1 clone by cultured LC ( cLC ) treated with these cytokines . ^^^ In addition , among the antibodies for CD 80 , CD 86 , CD 28 or CD 40 , the suppression of the Th 1 clone stimulation by LC was found to occur only with anti CD 80 and anti CD 28 antibodies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We analyzed the expression and function of the co stimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) during contact sensitivity reactions induced by the hapten 2 , 4 dinitrofluorobenzene ( DNFB ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Because rheumatoid synovium is characterized by an ongoing immune response , the expression and function of CD 80 , CD 86 , and other markers of DC differentiation by rheumatoid arthritis synovial APC were examined . ^^^ Whereas CD 80 expression by rheumatoid SF DC and monocytes was minimal , CD 86 was expressed by a subset of SF monocytes and by CMRF 44+SF DC . ^^^ CD 80 was expressed diffusely and at low levels by rheumatoid synovial tissue cells , whereas CD 86 was expressed by perivascular HLA DR+HLA DQ+CD80+CMRF 44+ DC , and by some CD14+ monocytes . ^^^ Anti CD 86 mAb and CTLA 4 Ig , but not anti CD 80 mAb , inhibited the MLR stimulated by SF DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression and function of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on human epidermal Langerhans cells . ^^^ We analyzed the expression and function of the two ligands for CD 28 , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , on human Langerhans cells ( LC ) , the antigen presenting cells from epidermis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
YT mediates lysis of CD80+ / CD86+ B lymphoblastoid cell lines and the murine mastocytoma p 815 transfected with CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Specific acceptance of cardiac allografts after treatment with antibodies to CD 80 and CD 86 in mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential expression of the costimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) in rheumatoid synovial tissue . ^^^ The latter may be provided by B7 . 1 ( CD 80 ) or B7 . 2 ( CD 86 ) on APC interacting with CD 28 on T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To determine if KS tumor cells can stimulate resting T cell proliferation , we asked whether the tumor cells express the critically important T cell costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD1a+ cells differed from CD14+ cells by ( 1 ) dendritic morphology , ( 2 ) higher expression of CD1a , CD1c , CD 4 , CD 40 , adhesion molecules ( CD11c , CD 54 , CD 58 ) , major histocompatibility complex ( MHC ) class 2 molecules and CD 28 ligands ( CD 80 and CD 86 ) , ( 3 ) lack of Fc receptor FcgammaRI ( CD 64 ) and complement receptor CR 1 ( CD 35 ) expression , and ( 4 ) stronger induction of allogeneic T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expansion of TIL was partially blocked by the addition of CTLA 4 Ig , which is an inhibitor of costimulatory molecules such as CD 80 and CD 86 , and was almost blocked by the addition of anti ( Fc receptor gamma 2 ) mAb . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , the combination of GM CSF and TNF alpha supports quantitative differentiation into dendritic cells , lacking c fms , CD 14 , and other macrophage properties , and expressing HLA DR , CD1a , CD 83 , CD 80 , CD 86 , and potent allostimulatory activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Opposing effects of CTLA 4 Ig and anti CD 80 ( B 7 1 ) plus anti CD 86 ( B 7 2 ) on experimental allergic encephalomyelitis . ^^^ The roles of the B 7 receptors , CD 80 and CD 86 , during actively induced experimental allergic encephalomyelitis were examined with specific monoclonal antibodies and CTLA 4 Ig . ^^^ Surprisingly , combined anti CD 80 + anti CD 86 monoclonal antibody injection on day 2 resulted in marked exacerbation of disease . ^^^ These results suggest distinct roles for CD 80 and CD 86 in the initiation of EAE , resulting in the diverse clinical outcomes observed in this model of EAE . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Neisserial porins increased the surface expression of the costimulatory ligand B 7 2 ( CD 86 ) but did not affect the expression of B 7 1 ( CD 80 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cross linking of CD 40 on directly isolated DC with mouse CD40L trimer ( mCD40LT ) markedly augmented CD 80 and CD 86 up regulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Two molecules capable of providing a costimulatory signal , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , have been shown to augment the immunogenicity of whole tumor cell vaccines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The interaction between CD 28 on T cells with CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on APCs is considered to be of critical importance for primary T cell activation both in vivo and in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Decreased inducible expression of CD 80 and CD 86 in human monocytes after ultraviolet B irradiation : its involvement in inactivation of allogenecity . ^^^ Costimulation by the CD 28 ligand CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) plays an important role during T cell proliferation by augmenting synthesis of interleukin 2 ( IL 2 ) and other cytokines . ^^^ In this study , we demonstrated induced expression of both CD 80 and CD 86 during allogeneic MLR , though human freshly isolated monocytes express CD 86 constitutively with a much lower level of CD 80 . ^^^ A monoclonal antibody ( MoAb ) against CD 86 , but not CD 80 , efficiently inhibited allogeneic T cell proliferative responses stimulated with highly purified monocytes . ^^^ The surface expression of CD 80 , CD 86 , CD 54 , and HLA DR on monocytes was further augmented by interferon ( IFN ) gamma ; this cytokine induced expression was dose dependently reduced by UV B irradiation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The binding of CD 40 ligand on activated T cells to CD 40 on resting B cells induces the expression of costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression and function of CD 80 and CD 86 costimulator molecules on synovial dendritic cells in chronic arthritis . ^^^ To examine CD 86 expression on dendritic cells isolated from the synovial fluid ( SFDC ) of patients with chronic arthritis , and to determine the importance of both CD 80 and CD 86 molecules in SFDC T lymphocyte interactions . ^^^ The costimulator activity of the SFDC CD 80 and CD 86 molecules was determined by allogeneic mixed lymphocyte reaction ( MLR ) . ^^^ CD 80 and CD 86 induction on SFDC during in vitro culture was also examined . ^^^ Fresh SFDC either lacked or showed very weak surface expression of CD 86 molecules ( as shown previously for CD 80 ) , yet contained CD 86 mRNA . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present report , we show that pre B cell ALL express alloantigen and adhesion molecules but uniformly lack B 7 1 ( CD 80 ) and only a subset express B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The differential role of CD 86 and CD 80 co stimulatory molecules in the induction and the effector phases of contact hypersensitivity . ^^^ We examined the distinct role between CD 80 and CD 86 on hapten induced CH in both an induction and an effector phase . ^^^ Intraperitoneal injection of mAb against CD 86 , but not CD 80 , 2 h before sensitization with epicutaneous application of dinitrofluorobenzene led to an almost complete inhibition of ear swelling , and histologically a marked reduction of edema , inflammatory polymorphonuclear cells and lymphocyte infiltration in the dermis . ^^^ In contrast , the administration with either anti CD 80 or CD 86 mAb 2 h before challenge partially inhibited CH reactions and a combination of both mAb did not improve the inhibitory effect . ^^^ Although Langerhans cells ( LC ) expressing MHC class 2 were observed in both the epidermis and dermis 24 h after primary sensitization , CD 86 ( + ) LC were observed only in the subepidermal regions and CD 80 bearing cells were not detected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
T lymphocyte receptor CTLA 4 binds costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) with high avidity and negatively regulates T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Analysis of the site of interaction of CD 28 with its counter receptors CD 80 and CD 86 and correlation with function . ^^^ Both receptors associate with the counter receptors CD 80 and CD 86 , but the avidity of interaction for CD 28 is about 20 fold lower than for CTLA 4 . ^^^ The conservative F substitution of Y 26 in the CDR 1 like region also reduced binding to CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On the other hand , activation of CD8+ cells by allogeneic MHC class 1 on EBV transformed B cells , which express two different CD 28 ligands , CD 80 and CD 86 , also proceeded very efficiently ( proliferation , cytotoxic activity and CD 25 expression ) , but was either not , or only partially , blocked by anti CD 80 and anti CD 86 MoAb or CTLA 4Ig . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , CD40L is the most potent stimulus in upregulating the expression of ICAM 1 , CD 80 , and CD 86 molecules on DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 , a CD 28 homologue expressed on activated T cells , binds with high affinity to the CD 28 ligands , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The gp 120 induced reduction in the proliferative responses of PBMC was at least partially reversed by the addition of IL 2 , anti CD 28 MAb , or transfectants expressing CD 80 , CD 86 , or CD 40 but not with exogenous IL 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The HSV IPC expression of CD 13 , CD 33 and Fc epsilon RI were weak but significant , while no CD 5 , CD11b , CD 16 , CD 64 , CD 80 or CD 86 were detected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
T cell costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are expressed in human microglia but not in astrocytes in culture . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 molecules ( CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) ) on APCs provide costimulatory signals for T cell proliferation . ^^^ When resting T cells were cocultured with CHO cells expressing B 7 1a molecules in the presence of PMA / ionomycin , T cell proliferation was not detected , while CHO cells either expressing CD 80 or CD 86 could promote the proliferation of resting T cells . in contrast to resting T cells , CHO cells expressing B 7 1a could support the proliferation of activated T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory effects of T cell proliferation during infection with human T lymphotropic virus types 1 and 2 are mediated through CD 80 and CD 86 ligands . ^^^ The modulation of expression of CD 80 and CD 86 on T cells following infection with human T lymphotropic virus ( HTLV ) I / II and its functional importance in T T cell interactions was examined . ^^^ Infection with HTLV I / II leads to constitutive expression of CD 80 and CD 86 , concomitant to down modulation of CD 28 on T cells . ^^^ The CD80 / CD86+ HTLV infected T cells stimulated proliferation of allogeneic and autologous resting T cells , which could be specifically blocked by a soluble CTLA 4Ig chimeric protein , anti CD 80 or anti CD 86 , but not by anti CD 54 . ^^^ It was necessary to inhibit interaction with both ligands ( CD 80 and CD 86 ) to optimally block HTLV mediated proliferation of allogeneic and autologous resting T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both precursor subsets mature at day 12 14 into DC with typical morphology and phenotype ( CD 80 , CD 83 , CD 86 , CD 58 , high HLA class 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Increased expression of CD 80 , CD 86 and CD 70 on T cells from HIV infected individuals upon activation in vitro : regulation by CD4+ T cells . ^^^ These ligands , CD 80 , CD 86 and CD 70 , are also expressed to some extent on activated T cells . ^^^ On the other hand , T cell stimulation in vitro induced high CD 80 , CD 86 and CD 70 expression on T cells from HIV infected individuals . ^^^ Indeed , high expression levels of CD 80 , CD 86 and CD 70 were found on activated CD8+ T cells from HIV individuals cultured in the absence of CD4+ T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Nef down regulated IL 2 , but not IL 4 produced upon induction by combinations of mitogens that mimicked TcR signals together with CD 28 mAb or CD 28 ' s natural ligand ( CD 80 and CD 86 ) . ^^^ However , the co signals provided by CD 28 to up regulate IL 2 induction were unaffected by Nef , since IL 2 produced by nef transfected cells was proportionally enhanced to the same extent as that of control cells , either upon stimulation by the CD 28 mAb or CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The B 7 receptors , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , on the Ag presenting cell ( APC ) , interact with T cell CD 28 or CTLA 4 to deliver a costimulatory signal , which is particularly important for Th 1 activation . ^^^ Using a soluble fusion protein ligand for the B 7 receptors , as well as specific monoclonal antibodies specific for either CD 80 or CD 86 , it has been demonstrated that B 7 costimulation plays a prominent role in determining clinical disease outcome in EAE . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interestingly , DC express a functional CD 40 , which triggering upregulates expression of CD 80 and CD 86 and induces cytokine production , indicating a reciprocal talk between DC and T cells during the course of antigen presentation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Requirement of CD 80 and CD 86 molecules for antigen presentation by eosinophils . ^^^ Furthermore , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) were expressed on the surfaces of untreated eosinophils . ^^^ Moreover , anti CD 80 or anti CD 86 MoAbs blocked proliferative responses of primed lymph node T cells and thymus T cells to SEB , and also blocked responses of primed lymph node T cells to OVA . ^^^ Thus , CD 80 and CD 86 play an important role in stimulation of T cells by eosinophils . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) provide costimulation through CD 28 , their ligand on the T cell . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In order to stimulate T cells efficiently , SE B cells had to be exposed to anti mu antibody , a treatment which induced expression of activation markers such as CD 80 , CD 86 , CD 69 and CD 39 , usually absent in resting SE B cells . ^^^ CD 80 and CD 86 molecules expressed by SE B cells participated in the chain of events required to promote the proliferation of allogeneic T cells as demonstrated by inhibition tests with the appropriate mAb . ^^^ The expression of CD 80 and CD 86 by anti mu treated SE B cells was not , however , the sole explanation for their good antigen presenting capacities since the exposure of FM B cells to anti mu antibody also induced expression of these surface structures . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Two distinct signals are required to activate T cells : an Ag specific signal and a costimulatory signal mediated primarily by B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) through interactions with CD 28 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
LC were CD1a+2 DR+2 , CD23+ , CD36+ , CD 80 , CD 86 , and CD 25 , while DC were CD1a+ / DR+3 , CD 23 , CD 36 , CD80+ , CD86+2 , and CD25+ , CD 40 and CD 32 were moderately expressed and nearly unchanged on maturation , in contrast to monocyte derived DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Infusion of anti B7 . 1 ( CD 80 ) and anti B7 . 2 ( CD 86 ) monoclonal antibodies inhibits murine graft versus host disease lethality in part via direct effects on CD4+ and CD8+ T cells . ^^^ We have found that the infusion of anti B7 . 1 ( CD 80 ) + anti B7 . 2 ( CD 86 ) mAb is effective in eliminating GVHD lethality induced by either CD8+ or CD4+ T cells . ^^^ Although anti CD 80 or anti CD 86 mAb individually were ineffective in preventing CD8+ T cell GVHD lethality , each mAb was partially effective in CD4+ T cell mediated GVHD . ^^^ These studies demonstrate that anti CD 80 + anti CD 86 mAb infusion is effective in preventing GVHD lethality by inhibiting donor CD4+ or CD8+ T cell expansion and provide the first evidence that CD 80 expression on donor T cells is critical for optimal GVHD lethality . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They bound human and murine CTLA 4 immunoglobulin ( CTLA 4 Ig ) fusion proteins indicating expression of CD 80 and or CD 86 . ^^^ The findings indicate that CD 80 and or CD 86 are involved in the stimulation of CD4+ and CD8+ alpha beta TCR+ T cells but not in the stimulation of either of the two gamma delta TCR+ populations . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Thus , CD 18 mAb inhibited the apoptotic response to IL 2 deprivation , whereas mAb against other adhesion molecules ( CD 28 , CD 29 , CD49d , CD 80 , CD 86 ) did not . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulation of human T cells by pCD 86 in both systems is as effective as costimulation by human CD 80 or CD 86 , and can be blocked by human CTLA4Ig . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Islet infiltrating t lymphocytes in insulin dependent diabetic patients express CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ While no biopsy revealed any islet cell specific CD 80 or CD 86 expression , biopsies from six of the nine patients with insulitis revealed both CD 80 and CD 86 expression on the islet infiltrating cells . ^^^ Of the CD 3 positive cells , 19 . 4 % expressed CD 80 and 21 . 7 % expressed CD 86 . ^^^ CD 80 and CD 86 positive cells were similarly distributed throughout the inflamed islets . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have shown previously that granulocyte macrophage colony stimulating factor stimulated mouse bone marrow derived MHC class II+ dendritic cell ( DC ) progenitors that are deficient in cell surface expression of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) can induce alloantigen specific T cell anergy in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In particular , we discuss here whether the type of antigen presenting cells ( APCs ) and the distinct expression of important adhesion molecules like CD 80 and CD 86 may influence the generation of CD4+ CTLs . ^^^ The level of CD 80 , but not of CD 86 , present on the APCs appears to be crucial for the induction of CD4+ CTLs . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of co stimulatory molecules CD 80 and CD 86 was suggested by the ability of mast cells to activate purified lymph node derived T cells . ^^^ The regulatory mechanisms of APC function by GM CSF and IFN gamma were investigated by measuring CD 80 and CD 86 transcripts in mast cells . ^^^ GM CSF treated must cells showed a strong increase in the expression of both CD 80 and CD 86 transcripts , whereas in IFN gamma treated mast cells , this expression was completely abrogated . ^^^ Thus , up and down regulation of CD 80 and CD 86 expression by GM CSF and IFN gamma is directly correlated to the APC function . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Appearance of cells expressing CD 80 and CD 86 costimulatory antigens in the pancreas of nonobese diabetic mice . ^^^ To evaluate the role of the CD 80 and CD 86 antigens in the development of insulin dependent diabetes mellitus , expression of these antigens in the pancreas of normal and nonobese diabetic mice was studied . ^^^ The pancreata of normal BALB / c mice and young nonobese diabetic mice contained no CD 80 or CD 86 positive cells . ^^^ CD 80 and CD 86 positive cells appeared in the pancreas of nonobese diabetic mice by 6 weeks of age . ^^^ The morphology of the CD 80 and CD 86 positive cells was heterogeneous . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
T lymphocyte receptors CD 28 and CTLA 4 bind costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on antigen presenting cells and regulate T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The testes of 4 week old NOD mice or normal BALB / c mice and the testis of 17 21 week old BALB / c mice contained no CD 80 or CD 86 expressing cells . ^^^ The CD80+ and CD86+ cells were often located adjacent to the vessel walls where a leukocyte not expressing CD 80 or CD 86 had attached to the endothelium . ^^^ The CD 80 and CD 86 antigens could not be observed in the same cells as judged from stainings in parallel sections . ^^^ The results suggest that T lymphocytes can not be activated locally in the testis of BALB / c and young NOD mice because of the absence of the necessary CD 28 ligands , CD 80 and CD 86 , from the APCs and because of the suppression of T lymphocytes by the testicular products . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that microglia with and without alloAgP activity expressed similar levels of major histocompatibility complex class 1 molecules ; however , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expression was primarily restricted to the alloAgP cell lines . ^^^ Monoclonal antibody ( Mab ) to CD 80 only partially blocked the proliferative response of allogeneic CD8+ T cells cocultured with the presenting cell lines , whereas Mab to CD 86 completely inhibited the response , indicating a significant role for this molecule in T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
There were no differences in expression of CD 80 and CD 86 surface molecules by dendritic cells that were either mock stimulated or stimulated with HIV 1 or HSV for 24 h . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CD28 / CTLA 4 ligands , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , provide a co stimulatory signal necessary for optimal T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 80 and CD 86 was enhanced by adding CD40L and IL 4 , and the DC showed stimulatory activity in MLC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The costimulatory molecules CD 80 and CD 86 affect the differentiation of Th 1 and Th 2 subsets in experimental allergic encephalomyelitis , an autoimmune disorder . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory function and expression of CD 40 ligand , CD 80 , and CD 86 in vascularized murine cardiac allograft rejection . ^^^ Alternatively , the ability of CD 40 stimulation to induce CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expression on antigen presenting cells ( APCs ) has led to the hypothesis that the role of CD 40 CD40L interactions in transplant rejection might be indirect , i . e . , to promote the costimulatory capacity of APCs . ^^^ Anti CD40L mAb has no detectable effect on CD 86 up regulation , but almost completely abolishes induction of CD 80 . ^^^ However , animals treated with anti CD 80 mAb or with a mutated form of CTLA4Ig ( which does not bind to CD 86 ) rejected their cardiac allografts , indicating that blockade of CD 80 alone does not mediate the graft prolonging effects of anti CD40L mAb . ^^^ These data support the notion that the role of CD 40 CD40L in transplant rejection is not solely to promote CD 80 or CD 86 expression , but rather that this pathway can directly and independently costimulate T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 co stimulatory regimes differ in their dependence on phosphatidylinositol 3 kinase : common co signals induced by CD 80 and CD 86 . ^^^ CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) ligation of CD 28 provide co stimulatory signals required for optimal lymphokine production in response to TCR zeta CD 3 ligation . ^^^ In this study , we have investigated whether CD 28 associated Pl 3 kinase is required for CD 80 and CD 86 co stimulation , as well as in co signaling that involves different primary signals ( i . e . ^^^ In the presence of anti CD 3 , ligation of CD 28 by both CD 80 and CD 86 was found to induce Pl 3 kinase recruitment and IL 2 production . ^^^ CD 80 and CD 86 therefore share a common signaling pathway leading to IL 2 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immunophenotypical studies have demonstrated frequent expression of lymphoid `` activation markers ' including CD 15 , CD 25 , CD 30 , CD 40 , CD 54 , CD 70 , CD 71 , CD 80 , CD 86 and MHC class 2 and less frequent expression of T or B cell associated antigens by the neoplastic H RS cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cause of such an anergic reaction is not clear since RS cells express HLA class 2 as well as the co stimulator molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulation with this mAb induced a similar level of proliferative responses of both B 1 and B 2 cells , as well as an increase in expression of cell surface molecules 1 A , CD 54 , CD 23 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Effect of CD 80 and CD 86 blockade and anti interleukin 12 treatment on mouse acute graft versus host disease . ^^^ We investigated the efficacy of a combination of anti CD 80 and CD 86 ( CD 80 + 86 ) monoclonal antibodies ( mAb ) , anti interleukin ( IL ) 12 mAb , or both , for prophylaxis in a mouse acute graft versus host disease ( GVHD ) model . ^^^ The treatment with a combination of anti CD 80 + 86 mAb efficiently reduced the lethality of GVHD , whereas mAb against either CD 80 or CD 86 alone had an effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cells showed morphologic and immunophenotypical features typical of DCs , including expression of major histocompatibility complex ( MHC ) class 1 and 2 molecules , CD1a , CD 80 , CD 86 , CD 13 , CD 33 , CD 40 , and CD 54 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The aim of this study was to investigate the mechanisms by which B 7 related costimulatory molecules ( CD 80 , CD 86 ) increase T cell responsiveness to extracellular ligands . ^^^ Indeed , experiments performed with purified subpopulations of antigen presenting cells and using B 7 transfected cell lines indicated that increased levels of CD 80 and / or CD 86 cell surface expression is associated with a faster kinetics of IL 2 production in response to SEB . ^^^ Accordingly , blocking of CD 80 or CD 86 derived signals by specific monoclonal antibodies led to a slower kinetics of IL 2 production in response to SEB . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , FACS analysis on day 7 PI showed that HIV did not affect differentiation of DC that survived CPE : apart from CD 4 downmodulation related to HIV production , overall expression of CD 40 , CD 80 , and CD 86 costimulatory molecules , and of HLA DR , was unchanged relative to controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blocking of both B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) was required in order to inhibit cytokine production , indicating that either molecule could act alone . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interaction of the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules on antigen presenting cells with the receptors CD 28 and CTLA 4 on T cells generates signals important in the regulation of immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Purified BDCs from cholestatic C57BL / 6 ( H 2b ) mice express not only the major histocompatibility complex ( MHC ) class 1 and class 2 antigens but also the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cultured DC also expressed high levels of class 1 and class 2 major histocompatibility complex , together with CD11c , DEC 205 , CD 80 , and CD 86 , markers characteristic of mature DC in general . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 , CD 86 and CD 40 provide accessory signals in a multiple step T cell activation model . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypic analysis showed that TFC , after incubation with interferon gamma , do not express the costimulatory molecules B 7 1 ( CD 80 ) and 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although the medullary foci lack central organization , the expression in these regions of CD 80 , CD 86 and CD 40 , as well as the clustering of dendritic cells , is similar to that observed in medullae of wild type mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules was closely regulated during development of the disease . ^^^ Expression of both CD 80 and CD 86 increased on cells in pancreatic lymph nodes in STZ treated C57BL / KsJ mice . ^^^ In wild type animals , treatment with mAb against CD 86 prevented , whereas treatment with mAb against CD 80 exacerbated , insulitis and hyperglycemia , indicating that mAbs against these molecules differentially affect development of disease . ^^^ CD 80 and CD 86 molecules , the CD28 / CTLA4 ligands , may have different roles in regulation of the disease and affect T cell function at steps beyond differentiation into mature phenotypes . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 ( B 7 1 and B 7 2 ) are the ligands on antigen presenting cells ( APCs ) which bind CD 28 and deliver the costimulatory signals necessary for T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of costimulatory molecules CD 80 and CD 86 in human carcinoma cell lines : its regulation by interferon gamma and interleukin 10 . ^^^ The costimulatory signal through CD 80 or CD 86 to its counterreceptor CD 28 or CTLA 4 on T cells has been shown to play an important role in the induction of T cell mediated immunity against tumors . ^^^ In the present study , we examined the expression of CD 80 and CD 86 in the cell lines derived from human gastric , esophageal and colorectal carcinomas at the mRNA level , by means of a reverse transcriptase / polymerase chain reaction analysis and , for their surface expression , using a flow cytometric analysis with monoclonal antibodies ( mAb ) . ^^^ The expression of mRNA for CD 80 or CD 86 was detected in all 18 cell lines tested , except for CD 86 on one cell line . ^^^ The cells from 13 ( 72 % ) or 12 ( 67 % ) of these cell lines expressed the surface CD 80 or CD 86 molecule , detected with the respective mAb . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To investigate expression of the co stimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on tumor associated dendritic cells ( TADCs ) , cryosections from basal cell carcinomas were immunostained . ^^^ In basal cell carcinomas , only 1 to 2 % of intratumor and 5 to 10 % of peritumor APCs expressed CD 80 or CD 86 . ^^^ In contrast , biopsies of immunological / inflammatory dermatoses revealed that 38 to 73 % of APCs expressed CD 80 and CD 86 . ^^^ When analyzed by flow cytometry , TADCs expressed high levels of HLA DR , but only 5 to 10 % co expressed CD 80 or CD 86 . ^^^ A 3 day culture in granulocyte / macrophage colony stimulating factor containing medium partially reconstituted the TADC expression of CD 80 and CD 86 as well as their immunostimulatory capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The interaction of CD 28 with B 7 molecules ( CD 80 or CD 86 ) is an essential second signal for both the activation of CD4+ T cells through the T cell receptor and the prevention of anergy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To study the functions of B 7 molecules on T cells , we transfected murine B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) cDNAs into the EL 4 T cell thymoma cell line and examined the transfectants for their ability to costimulate T cell proliferation in vitro and to induce antitumor immunity in vivo . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The structurally related T cell surface molecules CD 28 and CTLA 4 interact with cell surface ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on antigen presenting cells ( APC ) and modulate T cell antigen recognition . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition to an antigen specific signal , T cell activation requires an antigen independent costimulatory signal provided by interaction of CD 28 with B 7 ( CD 80 and CD 86 ) on the APC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Role of costimulators in T cell differentiation : studies using antigen presenting cells lacking expression of CD 80 or CD 86 . ^^^ For T cells to be optimally activated , recognition of Ag / MHC complexes by the TCR must be accompanied by a second , costimulatory signal that can be provided efficiently by the related costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ Recently , CD 80 and CD 86 have been implicated as differential determinants of Th 1 vs Th 2 type cytokine profiles . ^^^ To investigate the role of CD 80 and CD 86 in Th subset differentiation , we have examined the cytokine profiles induced in TCR transgenic T cells stimulated by peptide in association with splenic APCs obtained from knockout mice that selectively lack expression of either the CD 80 or the CD 86 molecule . ^^^ Our data suggest that CD 86 , and to a lesser extent CD 80 , can make significant contributions to the production of both IL 4 and IFN gamma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Therefore , we studied proliferation and cytokine production in highly purified CD8+ and CD4+ T cells from HIV infected and HIV subjects , induced by co activation via cell bound CD 80 , CD 86 and CD 40 or by allo activation . ^^^ Anti CD 3 combined with CD 80 or CD 86 induced a mixed Th1 / Th2 type cytokine profile in both CD4+ and CD8+ T cells from controls , whereas anti CD 3 plus CD 40 induced only low levels of Th 2 type cytokines and no interferon gamma ( IFN gamma ) in CD4+ T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 40 triggering of chronic lymphocytic leukemia B cells results in efficient alloantigen presentation and cytotoxic T lymphocyte induction by up regulation of CD 80 and CD 86 costimulatory molecules . ^^^ Freshly collected chronic lymphocytic leukemia B cells ( B CLL cells ) are known to be inefficient at stimulating allogeneic T cells , and to lack significant expression of B 7 ( CD 80 and CD 86 ) costimulatory molecules . ^^^ B CLL cells cocultured with human CD 40 ligand expressing mouse fibroblasts rapidly up regulated CD 54 and CD 58 adhesion molecules and B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulatory molecules , and acquired a strong stimulatory capacity towards CD4+ as well as isolated CD8+ allogeneic T cells . ^^^ Costimulation by both CD 80 and CD 86 proved critical for allogeneic T cell proliferation and CD 25 and HLA DR expression , since these were strongly inhibited by anti CD 80 or anti CD 86 monoclonal antibodies , and completely abrogated by CTLA 4 Ig fusion protein , which blocks both CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CTA 1 DD , as well as CT , up regulated expression of the CD 80 and CD 86 molecules on the targeted B cells , indicating that enhanced T cell costimulation may be responsible for the adjuvant effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Since the expression of either B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) on APC is required for the activation of T cells , it was important to establish human gastric epithelial cells expressed those surface ligands . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To investigate whether the immunosuppressing capacity of alpha MSH is associated with the up regulation of MC 1 , its effect on the expression of costimulatory molecules ( CD 86 and CD 80 ) on human monocytes was investigated . alpha MSH significantly inhibited the expression of CD 86 on LPS treated monocytes , which exhibited a high density of MC 1 , whereas CD 80 expression was not altered . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Prevention of primary T cell responses by immunotoxins directed against CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
For flow cytometric analysis , epidermal cell ( EC ) suspensions were prepared and double labeled with monoclonal antibodies against CD1a or HLA DR , and B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , ICAM 1 ( CD 54 ) , ICAM 3 ( CD 50 ) , LFA 3 ( CD 58 ) , E cadherin , or integrin beta 4 ( CD 104 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD4+ T cells costimulated with the A6H mAb upregulated CD 80 , CD 86 , CD 71 , interleukin 2 ( IL 2 ) R alpha , IL 2R beta and IL 2R gamma , while no corresponding up regulation of these cell surface molecules was seen in CD8+ T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on human hepatocellular carcinoma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The second , costimulatory signal can be provided by cell surface molecules on APCs such as B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , which interact with their counter receptors on T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Lack of CD 28 costimulation through CD 80 and CD 86 molecules on APC might play a causative role in anergy induction , as previously shown with T cell clones . ^^^ Addition of either CTLA 4Ig or blocking anti CD 80 and anti CD 86 mAbs , in combination with cyclosporin A , to cultures of PBMC with soluble Ag consistently resulted in Ag specific unresponsiveness , as evidenced upon antigenic rechallenge . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Inflammatory cytokines IFN gamma plus TNF alpha induce regulated expression of CD 80 ( B 7 1 ) but not CD 86 ( B 7 2 ) on murine fibroblasts . ^^^ Considerable experimental data now suggest that this costimulatory signal is generated predominantly by CD 28 when engaged by its ligands CD 80 ( B 7 1 ) and / or CD 86 ( B 7 2 ) . ^^^ Whether T cell activation is controlled in part by regulated CD 80 and / or CD 86 expression has been incompletely explored . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The inhibitory effect of interleukin ( IL ) 10 on the accessory cell function of AMs and the expression of CD 80 and CD 86 on AMs were also investigated . ^^^ Expression of CD 80 and CD 86 on AMs from these groups did not differ . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , costimulatory proteins on antigen presenting cells , bind to CD 28 on T cells . ^^^ In the experimental autoimmune encephalomyelitis model of MS , CD 80 on antigen presenting cells induces Th 1 cell responses ; CD 86 enhances generation of Th 2 cells . ^^^ Variation in CD 80 and CD 86 expression is likely to influence immune regulation in MS . ^^^ Therapy with IFN beta 1b markedly reduces the number of circulating CD 80 ( + ) B cells and increases CD 86 ( + ) monocyte number . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Molecular cloning and comparative analysis of the rhesus macaque costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model , we cloned and sequenced the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) costimulatory molecules from rhesus macaques . ^^^ Rhesus CD 80 and CD 86 were highly homologous to their human counterparts , with overall amino acid homologies of greater than 90 % , and were specifically recognized by murine antihuman CD 80 or CD 86 monoclonal antibodies . ^^^ Stable cell lines expressing rhesus CD 80 or CD 86 induced proliferation of suboptimally activated CD4+ T cells and transcription of cytokine mRNA . ^^^ Both CD 80 and CD 86 were able to provide costimulation for interferon gamma and IL 2 synthesis by rhesus CD4+ T cells , but CD 80 costimulation also resulted in synthesis of IL 4 and IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mitogenic stimulation of T cells reveals differing contributions for B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found by flow cytometry that purified human fibrocytes express each of the known surface components that are required for antigen presentation , including class 2 major histocompatability complex molecules ( HLA DP , DQ , and DR ) , the costimulatory molecules CD 80 and CD 86 , and the adhesion molecules CD11a , CD 54 , and CD 58 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Ligation of CD 28 on T cells with its natural ligands B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) provides a major costimulatory signal for T cells and is of potential importance for tumor rejection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The interaction between CD 28 and its ligands , CD 80 and CD 86 , is crucial for an optimal activation of antigen specific T cells . ^^^ However , the requirement of CD 80 or CD 86 co stimulation in Th 2 cell differentiation and activation is controversial . ^^^ Freshly isolated murine CD4+ and CD8+ T cells were incubated with P 815 transfectants expressing a similar level of either CD 80 or CD 86 in the presence of anti CD 3 mAb . ^^^ Both CD 80 and CD 86 co stimulated the proliferation of CD4+ and CD8+ T cells at comparable time kinetics and magnitude , but CD 86 alone was able to co stimulate IL 4 and especially IL 10 production in CD4+ T cells . ^^^ In typical Th 2 dependent immune responses elicited by Nippostrongylus brasillensis infection , the anti CD 86 mAb treatment but not the anti CD 80 mAb treatment efficiently inhibited antigen specific IgE and IgG 1 production , which was accompanied with the reduced IL 4 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Regulation of T and B cell responses by modulating interactions between CD28 / CTLA4 and their ligands , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Isolated IDC displayed a phenotypic profile similar to that of IDC in tonsils and spleen in situ , characterized by high level expression of major histocompatibility complex class 2 , the co stimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) , expression of the late DC maturation marker CD 83 , and no expression of CD1a , CD 13 , or CD 33 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 is a costimulation receptor that binds to the same ligands , CD 80 and CD 86 , as CD 28 with high affinity and is transiently expressed on the cell surface of activated T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Analysis of CD 80 and CD 86 expression on peripheral blood B lymphocytes reveals increased expression of CD 86 in lupus patients . ^^^ We screened peripheral blood mononuclear cells from 13 SLE patients , all having quiescent disease at the time of analysis , 12 allergy patients , and 21 normal subjects for the expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 , B 70 ) on small ( resting ) and large ( activated ) subsets of CD19+ B cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 28 , CTLA 4 , CD 80 , and CD 86 molecules in patients with autoimmune rheumatic diseases : implications for immunotherapy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CD 28 ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , are expressed on human tonsillar B cells , and their expression is up regulated by IL 4 , IL 13 , and / or an anti CD 40 monoclonal antibody ( mAb ) . ^^^ Conversely , the anti CD 80 and the other anti CD 86 mAbs tested had no effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To examine whether the ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) affect gene expression of Th 2 cytokines and development of Th 2 mediated pathologic tissue reactions , mice were treated with anti B 7 1 or anti B 7 2 at the time of sensitization to footpad inoculation of Schistosoma mansoni eggs or induction of pulmonary granuloma by i . v . injected eggs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are genetically and structurally related molecules expressed on antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential function of CD 80 and CD 86 transfected human melanoma cells in the presence of IL 12 and IFN gamma . ^^^ Introduction of co stimulatory molecules like CD 80 and CD 86 represents a means to augment the immunogenicity of tumor cells and to induce immune responses directed at tumor antigens . ^^^ Here we compared CD 80 and CD 86 transfected human melanoma cells to induce primary immune responses by their capacity to promote proliferation of human allogeneic resting T lymphocytes . ^^^ CD 80 and CD 86 transfected SkMel 63 melanoma cells induced T cell activation to a comparable degree , which was found to be independent of the cell surface density of these co stimulatory molecules . ^^^ Co expression of CD 80 and CD 86 did not result in a synergistic increase in T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , CD 80 and CD 86 plays differential roles for the development of autoimmune disease since helper T cell development is differentially affected by the blockade of either . ^^^ CD 80 or CD 86 in certain conditions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 on T cells and CD 80 and CD 86 on APC are key molecules in the maintenance and breakdown of anergy . ^^^ CTLA 4Ig fusion protein , that binds to both CD 80 and CD 86 with high affinity and thereby prevents interaction of CD80 / CD86 with CD28 / CTLA 4 , prevents or ameliorates several autoimmune disease in experimental animal models , supporting an importance of this pathway in the development of autoimmune diseases . ^^^ However , the studies using specific monoclonal antibodies against CD 80 and CD 86 have shown different outcomes in individual autoimmune models . ^^^ This suggests that the actual regulatory mechanisms of this pathway in autoimmunity is much more complex , because of the existence of two receptors ( CD 28 and CTLA 4 ) and two ligands ( CD 80 and CD 86 ) and the opposite function of CD 28 and CTLA 4 in T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The DC obtained from the cryopreserved PBMC expressed HLA DR , HLA DQ , CD 80 and CD 86 antigens , and stimulated allogenic PBMC to an extent almost identical to that obtained from fresh PBMC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interferon gamma differentially regulates CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) expression on human Langerhans cells . ^^^ CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) have recently been identified in cultured human Langerhans cells ( LCs ) , although their role and regulatory properties remain unclear . ^^^ We present our comparison of the expression of the molecules , mRNAs and the function between CD 80 and CD 86 in human LCs treated by interferon gamma ( IFN gamma ) . ^^^ We examined the regulatory properties of CD 80 and CD 86 expression in human LCs pretreated with IFN gamma . ^^^ Flow cytometric analysis indicated that the mean fluorescence intensity of CD 86 but not CD 80 was enhanced . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blockade of costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) down regulates induction of contact sensitivity by haptenated epidermal cells . ^^^ The hapten , trinitrobenzene sulphonic acid , induced weak B 7 1 ( CD 80 ) and moderate B 7 2 ( CD 86 ) expression on Langerhans cells and mRNA expression of both molecules in organ cultured murine skin . ^^^ When hapten treated epidermal cells were injected into mice after incubation with anti B 7 2 ( CD 86 ) or B 7 1 ( CD 80 ) antibody the resultant contact sensitivity reaction was decreased to less than 50 % of the control reaction , a reduction which was similar to that seen with the anti ICAM 1 and anti LFA 1 antibody induced inhibition of contact sensitivity . ^^^ Anti B 7 1 ( CD 80 ) or anti B 7 2 ( CD 86 ) antibody also inhibited hapten specific lymphocyte proliferation or the allogenic mixed lymphocyte and epidermal cell reaction in vitro , although the inhibitory effect of anti B 7 1 antibody was not as significant as that of anti B 7 2 antibody . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The majority of DC expressed high levels of class 2 MHC , expressed CD11c , and expressed the costimulator molecules CD 80 , CD 86 , and CD 40 ; CD 80 and CD 40 were further up regulated on culture . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On the other hand cells treated in vitro exhibit on the cell surface higher levels of MHC class 2 , of co stimulatory molecules ( CD 80 , CD 86 ) , of adhesion molecules ( CD 44 , intercellular adhesion molecule 1 ) , and acquire expression of the interdigitating DC surface marker NLDC 145 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The modulation of CD 80 and CD 86 expression on the HTLV 1 infected cells and its physiological role in the interaction of infected CD4+ T cells with uninfected CD4+ T cells was examined . ^^^ The HTLV 1 infected CD4+ T cell lines established from ATL patients and normal donors by infecting their CD4+ T cells with the virus expressed CD 80 , CD 86 , and HLA DR , and induced a proliferation of autologous and allogenic CD4+ T cells . ^^^ While the CD4+ T cells stimulated with the autologous HTLV 1 infected cells for 7 days expressed CD 80 and CD 86 but not HTLV 1 gene products , they expressed HTLV 1 gag antigen after 4 weeks . ^^^ The interaction of HTLV 1 infected and uninfected CD4+ T cells was profoundly suppressed by a combination of CD 80 and CD 86 monoclonal antibodies . ^^^ These results suggest that the induction of CD 80 and CD 86 on HTLV 1 infected CD4+ T cells participates actively in the generation of the virus infected progenitor cells . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Characterization of rat CD 80 and CD 86 by molecular cloning and mAb . ^^^ In the present study , we cloned rat CD 80 and CD 86 cDNA from a HTLV 1 transformed rat T cell line , Lewis S 1 , expressing a high level of CTLA 4 Ig binding proteins . ^^^ The predicted CD 80 and CD 86 polypeptides were composed of 321 and 313 amino acids respectively , and exhibited features common to human and mouse B 7 family proteins . ^^^ Both CD 80 and CD 86 mRNAs were abundantly detected in HTLV 1 transformed rat T cell lines but not in a thymic lymphoma cell line . ^^^ To further explore the function of rat CD 80 and CD 86 , we generated cDNA transfectants and anti rat CD 80 ( 3H5 ) and anti rat CD 86 ( 24F ) mAb . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The inhibitory effects of TGF beta 1 on CD4+ cells are not mediated by alterations in APC costimulation since TGF beta 1 did not inhibit the Ag induced expression of MHC class 2 molecules , CD 80 or CD 86 on splenic APC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The costimulatory genes Cd 80 and Cd 86 are linked on mouse chromosome 16 and human chromosome 3 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have developed expression cassettes for cell surface markers CD 80 and CD 86 , two functionally related costimulatory molecules that play an important role in the induction of T cell mediated immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We used a neutralizing antibody to block CTLA 4 interaction with its ligands CD 80 and CD 86 during infection of mice with the nematode , Nippostrongylus brasiliensis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , no correlation is found between the percentage of CD 80 and CD 86 positive AM in these same patients . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mutation of these residues , which are conserved in the CTLA 4 / CD28 family , significantly reduces binding to CD 80 and / or CD 86 , implicating this patch as a ligand binding site . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Hence , the binding of CD 28 on T cells to both CD 80 and CD 86 ligands represents a crucial initial costimulatory step leading to accelerated graft rejection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The heterogeneous expression of CD 80 , CD 86 and other adhesion molecules on leukemia and lymphoma cells and their induction by interferon . ^^^ CD 80 ( B7 / BB 1 , B 7 1 ) and CD 86 ( B 70 , B 7 2 ) take an important role in the interaction between T lymphocytes and antigen presenting cells ( APCs ) as co stimulatory molecules . ^^^ We analyzed the manifestation of adhesion molecules , including CD 80 and CD 86 , on some leukemia and lymphoma cells . ^^^ Constitutive expression of CD 80 and / or CD 86 was frequently observed on B cell leukemia / lymphoma cells , while it is rare on myeloid leukemia cells . ^^^ Our data implies the immunotherapy via CD 80 and CD 86 for patients with hematological malignancies and the possibility to enhance it using interferons . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The interaction of CD 28 with one of the B 7 molecules ( CD 80 and CD 86 ) on professional antigen presenting cells ( APC ) is generally considered as the most important co stimulatory signal for T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential regulation of human , antigen specific Th 1 and Th 2 responses by the B 7 homologues , CD 80 and CD 86 . ^^^ A selectivity of B7 . 1 ( CD 80 ) for promoting Th 1 responses and B7 . 2 ( CD 86 ) for promoting Th 2 responses in the murine system has recently been suggested . ^^^ Neither independent efficacy nor synergy with anti CD 86 was apparent with anti CD 80 treatment ; two different anti CD 80 blocking antibodies yielded identical results . ^^^ Conversely , antigen specific Th 1 and Th 2 clones were insensitive to treatment with either anti CD 80 , anti CD 86 , or a combination of the two . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules are costimulatory molecules that are indispensable to T cell activation by antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cultured human decidual stromal cells express B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) and stimulate allogeneic T cells . ^^^ In the present study , we show by flow cytometry that these cells also express B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , two antigens involved in the costimulation of T cells by antigen presenting cells . ^^^ Using an immunohistochemical technique , we found that in cryostatic sections of early human decidua , CD 80 and CD 86 were expressed mainly by DSC located around the decidual vessels , a location compatible with preDSC rather than fully differentiated DSC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , DCs incubated for 48 h with multivalent sFc gamma RIII expressed increased levels of CD 40 , CD 80 , CD 86 , CD 54 , CD 58 , HLA class 1 and class 2 molecules and decreased levels of CD 23 and CD 32 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
As expected these DC expressed the relevant cell surface molecules including HLA DR , CD 58 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Small percentages of plasma cells expressed weak but detectable levels of HLA DR , HLA DQ , CD 40 , CD 80 , and CD 86 , which could be upregulated by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Characterization of accessory molecules in murine lung dendritic cell function : roles for CD 80 , CD 86 , CD 54 , and CD40L . ^^^ Lung DCs expressed surface molecules typical of DCs from other sites including CD11a , CD 54 , CD 80 , and CD 86 . ^^^ As is true for DCs in other sites , costimulatory molecules including CD 80 , CD 86 , CD40L , CD 2 , CD 54 , and CD11a played important roles in lung DC initiated T cell proliferation . ^^^ Interestingly , anti CD 86 monoclonal antibody ( mAb ) had little inhibitory effect on the MLR unless it was added in combination with anti CD 80 mAb . ^^^ These studies suggest that CD 80 on lung DCs can provide a costimulatory signal to allogeneic T cells in the absence of CD 86 signaling , but that CD 86 functions poorly except when CD 80 is also engaged . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypic analysis of cultured DCs from BM augmented patients , unlike that of controls , exhibited a marked down regulation of B 7 1 ( CD 80 ) while retaining normal levels of expression of B 7 2 ( CD 86 ) cell surface molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are indispensable for T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometry investigation of phenotypic markers including CD1a , HLA DR , CD 80 ( BB1 / B7 . 1 ) and CD 86 ( B70 / B7 . 2 ) , and functional status ( stimulatory potential in allogeneic mixed leucocyte reactions ( MLR ) ) confirmed the generation of cells phenotypically identified as cultured DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blockade of the interactions between CD 28 and its ligands , CD 80 and CD 86 , prolongs the survival of allografts in some transplantation models . ^^^ However , we found that allogeneic hepatocytes were completely rejected within 7 days after intrasplenic transplantation , even when treated with monoclonal antibodies ( mAbs ) against CD 80 and CD 86 ( anti CD80 / 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential distribution of B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) costimulatory molecules on mucosal macrophage subsets in human inflammatory bowel disease ( IBD ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using flow cytometry and reverse transcription PCR analyses , the expression of molecules belonging to the tumor necrosis factor receptor ( TNFR ) and tumor necrosis factor ligand ( TNFL ) families , as well as the expression of CD 80 and CD 86 molecules , was analyzed in normal B cells and in different chronic lymphoproliferative disorders of B cell type , including B cell chronic lymphocytic leukemia ( CLL ) , mantle cell lymphoma , hairy cell leukemia ( HCL ) , and HCL variant . ^^^ TNFR 2 ( CD120b ) and CD 80 were distributed on neoplastic B cells from all groups , usually at an intermediate to high degree of intensity , whereas the CD 86 molecule was present at lower intensity than CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
G 7 DC not only expressed class 1 and class 2 MHC , CD1a , CD11c , CD 23 , CD 40 , CD 54 , CD 58 , CD 80 , CD 86 and CD 95 , like G 4 DC , but also CD 21 , which is the complement receptor type 2 , a ligand for CD 23 and a receptor for EBV and IFN alpha . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on APC provide a major costimulatory signal through interactions with CD 28 on T cells . ^^^ Absent from resting human T cells , CD 86 is up regulated early upon T cell activation , whereas CD 80 expression appears later . ^^^ Whereas T cell expression of CD 80 has been implicated in costimulation , the functional significance of CD 86 expression on T cells is unclear . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Transduced or untransduced dendritic cell progeny expressed comparable levels of HLA DR , CD 83 , CD1a , CD 80 , CD 86 , S 100 , and p 55 antigens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cultured cells from the HIV 1 infected subjects had similar morphology and phenotype of mature DC ( CD 80 = 41 + / 8 % , CD 86 = 77 + / 5 % , CD 40 = 87 + / 6 % , CD1a = 1 + / 1 % ) to DC cultured from seronegative subjects . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both types of Mo DC expressed OX40L after stimulation through CD 40 ; ligation of OX40L on activated IL 4 Mo DC enhanced by 4 to 35 fold their cytokine production ( TNF alpha , IL 12 p 40 , IL 1 beta , and IL 6 ) and increased CD 80 , CD 86 , CD 54 , and CD 40 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cells from both sources were DEC 205+ , CD11c+ , MHC class II+ , CD 80 ( low ) ( i . e . , low level of CD 80 ) , CD 86 ( low ) and CD 40 ( low ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CTLA 4 ligands CD 80 and CD 86 were simultaneously expressed in most CTLA 4 negative lymphoma cases . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Best understood of these costimulatory interactions are those between CD 28 and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Under these culture conditions , by day 21 , a fraction of cells had lost CD 14 and acquired both CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 ( CD 152 ) , high avidity receptor for CD 80 and CD 86 , is a powerful regulator of T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD34+ and monocyte derived DCs were characterized by a similar proportion of CD1a+ cells expressing HLA class 2 antigens and CD 54 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Finally , we found that the virulent Listeria induced macrophages expressed a higher level of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules than did the avirulent Listeria induced macrophages and naive peritoneal macrophages . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The percentage of B cells expressing CD 80 , CD 86 , and the level of expression of CD 20 is higher in blood than in LN . ^^^ In contrast to humans , a relatively high proportion of T cells in blood also express the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Ligation of CD 40 on antigen presenting cells by its cognate ligand CD40L is thought to induce expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BACKGROUND : Small , resting B cells ( rB cells ) express major histocompatibility complex ( MHC ) class 2 molecules but not the putative costimulatory molecules , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) ; they are classified as nonprofessional antigen presenting cells . rB cells have been shown to be capable of anergizing T cells in vitro and inducing the prolonged survival of skin grafts mismatched for a single minor histocompatibility ( miH ) antigen , H Y . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here we show that virtually all germinal center ( GC ) derived B cell lymphomas including follicular lymphoma ( FL ) and diffuse large cell lymphoma , but not mantle cell lymphoma or small lymphocytic lymphomas ( SLL / CLL ) , express B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on their cell surface in situ , although at extremely low levels . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Because the B7 / CD28 costimulatory pathway has been shown to influence the differentiation of Th cell subsets , we investigated the contribution of the B 7 molecules CD 80 and CD 86 to the Th 2 cytokine profile and development of arthritis in BALB / c mice infected with Borrelia burgdorferi . ^^^ Moreover , combined CD80 / CD86 blockade by using anti CD 80 and anti CD 86 monoclonal antibodies or CTLA 4Ig enhanced IFN gamma production over that seen with CD 86 blockade alone , yet augmentation of this Th 1 associated cytokine did not enhance disease . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The second , costimulatory signal can be provided by cell surface molecules on antigen presenting cells such as B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , which interact with CD 28 on T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 80 , CD 86 , and ICAM 1 on lymphoma cells was up regulated by soluble CD 40 ligand . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In these in vivo studies , CD 28 costimulation did not show a significant benefit , possibly because of the high level expression of CD 80 and CD 86 on the surface of the lymphoma cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : With flow cytometric analysis we have investigated the expression of the costimulatory molecules B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and CD 18 and the intercellular adhesion molecule 1 ( ICAM 1 ) on peripheral blood monocytes and the expression of the activation markers HLA DR and IL 2R ( CD 25 ) on peripheral blood T lymphocytes from 31 CD patients , 17 ulcerative colitis ( UC ) patients , and 10 healthy controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we confirm the absence of immune costimulatory molecules , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , on leukaemia cells and demonstrated that these costimulatory molecules on the leukaemia cells can be upregulated by a combination of GM CSF and IL 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The Mox 2 gene maps to Chr 16 , telomeric to the clustered T cell costimulatory molecules Cd 80 and Cd 86 ( Reeves et al . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 25 ( IL 2 receptor alpha chain ) and CD 28 , characteristic of T cells , were detected on the surface of these cells ; this latter finding rises the possibility that enterocytes could be activated by IL 2 and / or via CD 28 through binding to its ligands CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Equivalent levels of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were found on the two DC subtypes and co stimulator assays did not reveal any functional differences between them . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Role of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) in the immune response to an intracellular pathogen . ^^^ The costimulatory ligands CD 80 and CD 86 play a crucial role in the initiation and maintenance of an immune response . ^^^ We demonstrate that whereas infection of human monocytes with viable tachyzoites of Toxoplasma gondii resulted in rapid induction of expression of CD 80 and up regulation of expression of CD 86 , incubation with killed organisms failed to alter the levels of expression of these costimulatory ligands . ^^^ Proliferation of resting T cells in response to parasite infected cells was dependent on both CD 80 and CD 86 . ^^^ More importantly , early production of IFN gamma in response to T . gondii by T cells from T . gondii seronegative individuals occurred only after stimulation with monocytes that exhibited increased expression of CD 80 and CD 86 ( monocytes infected with viable parasites ) and was almost completely ablated by the combination of anti CD 80 plus anti CD 86 mAb . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cryptococcus neoformans differently regulates B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression on human monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show that hepatocytes which do not express CD 80 and CD 86 co stimulatory molecules are able to induce activation and effective proliferation of specific naive CD8+ T cells in the absence of exogenously added cytokines , a property only shared by professional antigen presenting cells ( APC ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The individual roles of CD 80 and CD 86 in autoimmune diseases are complicated by multiple factors in vivo . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that expression of costimulatory molecules such as CD11a , CD 40 , CD 54 , CD 58 , CD 80 and CD 86 did not vary significantly between caM phi and aaM phi and was low for CD 58 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 12 reverses established antigen specific tolerance of contact sensitivity by affecting costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On culture with granulocyte macrophage CSF , this population differentiated into dendritic cells expressing CD 83 , CD 80 , HLA DR ( bright ) , CD 86 , and CD 54 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blockade of the ligands for CD 28 and CTLA 4 , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , revealed distinct and nonoverlapping function . ^^^ Blockade of CD 80 at initial immunization completely abrogated primary and secondary Ab responses , whereas blockade of CD 86 suppressed primary but not secondary responses . ^^^ Simultaneous blockade of CD 80 + CD 86 was less effective at suppressing Ab responses than either alone . ^^^ These findings suggest complex and distinct roles for CD 28 , CTLA 4 , CD 80 , and CD 86 in T cell costimulation following nucleic acid vaccination . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of CD 80 and CD 86 was up regulated in the memory B cells , compared to naive B cells and plasma cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypically , all the induced cell types expressed CD1a , CD 80 , CD 86 , HLA DR , and CD 68 in a magnitude similar to that of non treated monocyte derived DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These molecular changes include neo expression of the DC associated surface molecules cluster of differentiation ( CD ) 1a , CD1b , CD1c , human leukocyte antigen ( HLA ) DR , HLA DQ , CD 80 , CD 86 , CD 40 , CD 54 , and CD 5 , and downregulation of CD 15 and CD65s . ^^^ Additional stimulation with CD 40 ligand induces also expression of CD 83 and upregulates CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells expressed class 1 and class 2 MHC , CD1a , CD 2 , CD 4 , CD11c , CD 40 , CD 45 , CD 80 , CD 83 , and CD 86 and were potent stimulators of allogeneic T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that the accessory cell activity of monocytes during LPS induced T cell proliferation is characterized by the following features : LPS primed monocytes are competent stimulators of T cell proliferation ; interaction of LPS with monocytes during the priming step is dependent on CD 14 and is sensitive to ammonia ; monocyte / T cell interactions are not MHC restricted but are strongly dependent on interactions of CD 28 and / or CTLA 4 on T cells and their ligands CD 80 and / or CD 86 on monocytes . ^^^ CD 80 seems to be crucial for the activation of T cells by monocytes , since monocytes expressing CD 86 but not CD 80 after LPS stimulation were unable to stimulate T cells ; IL 12 , at least as a costimulatory factor , but not IL 15 , is important in LPS induced T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A crucial role of host CD 80 and CD 86 in rat cardiac xenograft rejection in mice . ^^^ METHODS : We investigated the differential roles for direct and indirect antigen presentation by preventing the CD 28 costimulatory pathway with monoclonal antibodies to rat or mouse CD 80 and CD 86 in a rat to mouse cardiac transplantation model . ^^^ RESULTS : Although the mouse anti rat monoclonal antibodies to CD 80 and CD 86 did not significantly prolong the survival of rat cardiac xenografts in mice , the rat anti mouse monoclonal antibodies to CD 80 and CD 86 did prolong the survival . ^^^ CONCLUSIONS : These findings suggest that the indirect antigen presentation , which is mediated by CD 80 and CD 86 pathway on host APC , plays a crucial role in concordant cardiac xenograft rejection . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 shares important sequence homology with CD 28 and binds to the same ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ We have developed new monoclonal antibodies against human CTLA 4 and have investigated the in situ expression of CTLA 4 in a wide variety of normal and pathological human tissues expressing CD 80 and CD 86 . ^^^ Interestingly , no or at most scarce expression of CTLA 4 was found in granulomatous lymph nodes , T cell mediated inflammatory diseases , or non Hodgkin ' s lymphomas , regardless of their expression of CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Examination of the surface expression of MHC class 2 , adhesion molecules ( CD11a , CD11b , CD 48 , CD 54 , and CD 102 ) or costimulatory molecules ( CD 24 , CD 80 , and CD 86 ) showed no differences between male and female derived Mac 3+ APC populations . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Several in vitro and in vivo models have demonstrated a role for the costimulatory molecules , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , in the generation of distinct effector T cell responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The inhibitory effects of T : T presentation were not due to a lack of costimulation , since the T cells expressed levels of CD 80 and CD 86 higher than those detected on cultured dendritic cells and equipped them to function as efficient APCs for primary CD8+ T cell responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both types of DC expressed high levels of CD1a , MHC class 2 , CD 80 , CD 86 and CD 40 and were potent stimulators of LY proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Thus , the expression of IL 7 by DC follows the pattern described previously for CD 80 , CD 86 and CD 40 . ^^^ However , tissue culture of purified resting / immature DC , in contrast to CD 80 , CD 86 and CD 40 , failed to induce IL 7 transcripts . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After 24 hr of culture , they upregulated the expression of MHC 2 , CD 40 , CD 80 , and CD 86 to levels just as high as those on DCs from organ transplant donors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immunoliposomes containing monoclonal antibodies ( MAbs ) to the costimulatory molecules CD 28 and CTLA 4 and their counterreceptors B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were evaluated for the ability to increase the immune response to recombinant envelope protein rgp 120 of the MN strain of human immunodeficiency virus type 1 ( HIV 1 ) during vaccination . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Functional consequences of dysregulated B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression in B or T lymphocytes of transgenic mice . ^^^ We have generated transgenic mouse strains that constitutively express B 7 1 ( CD 80 ) at high levels on B cells or T cells or express B 7 2 ( CD 86 ) on T lymphocytes to examine the consequences of dysregulated B 7 expression on T cell responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This was not related to binding of CTLA4Ig or CD40L fusion proteins , implying similar levels of expression of their ligands , CD 80 and CD 86 or CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Overnight cultured DC from livers of FL treated animals expressed both higher levels of costimulatory molecules ( CD 80 and CD 86 ) and allostimulatory activity than those from controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The absence of autologous tumour specific cytolytic T cell ( CTL ) activity may be due to the poor immunogenicity of colorectal tumour cells , which we found expressed only low levels of MHC 1 antigens and CD 54 and failed to express MHC 2 antigens or the co stimulatory molecules CD 80 , CD 86 or CD 106 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recent studies have suggested that the accessory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) differ in their capacity to generate Th 1 vs Th 2 responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Growth and differentiation of DC was followed by an increase in expression of MHC 2 and co stimulating molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
One clone showed sequence homology to a gene encoding a molecule on rat dendritic cells ( MRC OX 2 ) , with homology to genes encoding the costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules ( CD 80 and CD 86 ) on Reed Sternberg cells are associated with the proliferation of background T cells in Hodgkin ' s disease . ^^^ To elucidate the relationship between Reed Sternberg ( R S ) cells and background T cells , the expression of CD 80 and CD 86 of R S cells in Hodgkin ' s disease ( HD ) , and the ligand CD 28 expression and the MIB 1 index of background T cells were immunohistochemically investigated . ^^^ CD 80 and CD 86 were found to be expressed on R S cells in almost all cases of HD . ^^^ These results suggest that the interaction between CD 80 and CD 86 on R S cells , and CD 28 on background T cells may induce T cell proliferation and be associated with tumor mass of HD . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules implicated in differential Th1 / Th2 commitment CD 80 , CD 86 , and IL 12 are not inducible by SR ligation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of B 7 family costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on tumor cells enhances host immunity . ^^^ A critical test for the hypothesis is whether selective engagement of CTLA 4 receptors by their natural ligands CD 80 and CD 86 enhances or reduces antitumor immunity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In immunohistochemical analysis , CD 80 and / or CD 86 were detected on dendritic shaped cells not only in the epidermis but also in the dermis in the inflammatory lesions of atopic dermatitis ( n = 12 ) . ^^^ CD 80 was expressed in only five cases ( 42 % ) , while CD 86 was expressed in all cases ( 100 % ) . ^^^ In non lesional skin of atopic dermatitis ( n = 4 ) , CD 86 but not CD 80 was detected in one case . ^^^ The CD 80 or CD 86 positive cells were confirmed as Langerhans cells by double immunostaining using anti CD1a monoclonal antibody . ^^^ Neither CD 86 nor CD 80 was detected on keratinocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A stimulating anti CD 28 mAb , or CD 80 or CD 86 on stably transfected P 815 cells , provided the co stimulatory signal . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
PURPOSE : To examine normal and inflamed conjunctiva from patients with ocular cicatricial pemphigoid ( OCP ) for the presence of costimulatory molecule CD 28 and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This observation emerged from studies aimed at using retrovirus vectors to genetically modify tumor cells to express the costimulatory proteins CD 80 and CD 86 . ^^^ Retrovirus mediated expression of CD 80 and CD 86 in T lymphoblastoid CEM cells resulted in an unexpected intracellular focal concentration of both proteins in the genetically modified cells . ^^^ By extending these studies to an analysis of CD 80 and CD 86 expression in PBMC , we observed that endogenous CD 86 expression in peripheral blood monocytes also involved a similar intracellular focal concentration of the protein . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
When compared with non TNFalpha supplemented cultures , these DC also displayed an increased surface expression of CD 83 as well as the costimulatory molecules , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Activation markers such as CD 10 , CD 38 , and CD 95 and T cell costimulatory molecules CD 80 and CD 86 , which were expressed by neoplastic follicles , were either downregulated or absent in the interfollicular component in most of the cases . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendritic cells that developed from myeloid precursors accumulated after 2 weeks in an intermediate CD1a , CD 80 , CD 83 , CD 86 stage . ^^^ This maturation involved increased expression of CD 80 , CD 83 , CD 86 , CMRF 44 , HLA A , B , C , and DR as well as downregulation of CD1a and CD11b . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Co stimulatory molecules B 7 1 ( CD 80 ) / B7 2 ( CD 86 ) and their counter receptors CD28 / CTLA 4 play pivotal roles in T cell activation and immune regulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Biased dependency of CD 80 versus CD 86 in the induction of transcription factors regulating the human IL 2 promoter . ^^^ One such co stimulatory signal is delivered when CD 28 on T cells binds to CD 80 or CD 86 on antigen presenting cells ( APC ) . ^^^ In the present study , we analyzed the ability of CD 80 and CD 86 to co stimulate human T cells activated by superantigen . ^^^ Using the Raji B cell lymphoma , which express similar levels of CD 80 and CD 86 , it was found that T cell proliferation was mainly co stimulated by CD 80 . ^^^ To further characterize the consequences of this biased co stimulatory dependency , we employed a well defined system of transfected CHO cells expressing human MHC class 2 together with CD 80 , CD 86 or CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The consequence of autoantigen presentation by thyroid cells is dependent on the magnitude of expression of both HLA class 2 antigens ( mainly HLA DR ) and costimulatory molecules , such as B 7 ( CD 80 and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This was significantly correlated with the expression of CD 28 ligand , i . e . , CD 86 ( but not CD 80 ) , and with an increase in the proliferative activity ( labeling index ) of myeloma cells in bone marrow . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on cerebrospinal fluid cells in multiple sclerosis . ^^^ The costimulatory CD 80 and CD 86 molecules were measured by flow cytometry on cerebrospinal fluid ( CSF ) and blood lymphocytes from patients with possible first attacks of multiple sclerosis ( MS , n = 25 ) , clinically definite MS ( n = 16 ) , and noninflammatory neurological disease control subjects ( n = 30 ) . ^^^ In patients with demyelinating diseases more CSF B cells expressed CD 80 than in control subjects whereas the expression of CD 86 by T cells in CSF was low in patients with demyelinating disease and highly variable in the control subjects . ^^^ In patients with possible first attacks of MS the expression pattern of CD 80 and CD 86 differed significantly between patients with or without intrathecal synthesis of IgG . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Despite the importance of the costimulatory proteins B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and their counterreceptors CD 28 and CTLA 4 ( CD 154 ) in the regulation of T cell proliferation in the adult immunological system , the initial appearance of these proteins during embryonic development has not been investigated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To determine whether B 7 signals are associated with atopic responses in man , we assayed CD 80 and CD 86 expression on B cells and monocytes from atopic patients and controls . ^^^ Peripheral blood mononuclear cells from 10 patients with perennial allergic rhinitis and from 10 normal subjects were cultured in the presence or absence of house dust mite antigen , and B cells and monocytes were assayed for expression of CD 80 and CD 86 by flow cytometry . ^^^ CD 86 on B cells was significantly and selectively upregulated in all atopic subjects , but not in normal subjects , whereas CD 80 expression was not altered in B cells from the atopic subjects or controls . ^^^ In contrast , both CD 80 and CD 86 were upregulated in monocytes from the atopic subjects as well as the controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells are directly transfected in vivo , present Ag , and display the surface proteins CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using transfected variants of the human colorectal cancer cell line SW 480 we tested various costimulatory molecules ( CD 80 , CD 86 , CD 54 ) and a class 2 major histocompatibility complex ( MHC ) allele ( HLA DR 3 ) alone or in combination on their ability to support primary T lymphocyte activation in vitro . ^^^ Expression of CD 80 or CD 86 similarly as the combination of both was not sufficient to induce proliferation of human allogeneic T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CpG ODN caused up regulation of MHC class 2 , CD 40 and CD 86 , but not CD 80 on immature and mature DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 16 cells and their transfected sublines were CD40+ CD44+ but expressed no ( or low levels of the ) costimulator molecules CD 154 ( CD40L ) , CD 48 , CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Circulating CD34+ cells were purified to > or =98 % by high gradient magnetic separation and then analyzed for the coexpression of HLA DR , the common beta chain of the leukointegrin family CD 18 and costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ Three color staining cytofluorimetry indicated that expression of CD 80 and CD 86 were upregulated in 6 . 9 + / 4 . 9 and 10 . 7 + / 2 . 6 % CD34+ blood cells respectively , after 24 30 h of culture with autologous or allogeneic mononuclear cells , or with CD4+ , or CD8+ T cells , but not with medium alone . ^^^ Blocking experiments demonstrated that preincubation of stimulator and responder cells with anti CD 80 plus anti CD 86 monoclonal antibodies induced a 84 + / 8 % inhibition of CD34+ cell allostimulating activity after 6 days in primary MLC . ^^^ These results suggest that G CSF mobilized CD34+ hematopoietic progenitors with alloantigen presenting function express CD 18 and may upregulate CD 80 and CD 86 upon interaction with T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In particular , the relative competence of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) to provide effective co stimulus is not well defined , and the identification of the T cell co stimulatory receptor that mediates B 7 co stimulation in tumor rejection has not been addressed . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric triple fluorescence analysis was applied to study CD 14 , CD 16 , CD 33 , CD 44 , CD11b , CD11c , CD 64 , HLA DR , CD 80 , CD 86 and CD3 / CD19 expression . ^^^ Furthermore , expression of CD 80 ( 9 . 2 + / 4 . 2 % ) and CD 86 ( 15 . 1 + / 7 . 3 % ) was low , suggesting a low ability of normal intestinal macrophages to activate T cells and T cell mediated immune responses . ^^^ These cells exhibit a single phenotype in normal mucosa ( CD33++ , CD44++ , CD 14 , CD 16 , CD11b , CD11c , HLA DRlow , CD 80 , CD 86 ) lacking lipopolysaccharide ( LPS ) receptor and costimulatory molecules . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 40 ligand ( CD40L ) and B 7 2 ( CD 86 ) were also expressed , but to a lower extent , while B 7 1 ( CD 80 ) expression was limited . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : DCs isolated from livers of FL treated donor mice exhibited increased cell surface expression of CD 40 , CD 80 , CD 86 , and IAb , and augmented T cell allostimulatory activity compared with controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 86 ( B 7 2 ) , but not CD 80 ( B 7 1 ) , expression in the epidermis of transgenic mice enhances the immunogenicity of primary cutaneous Candida albicans infections . ^^^ Transgenic ( Tg ) mice whose epidermal keratinocytes constitutively overexpress either B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) exhibited exaggerated cutaneous delayed type hypersensitivity ( DTH ) to haptens compared to non Tg mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We studied the surface expression of B 7 1 and B 7 2 in 24 cases of gastric carcinoma from the primary locus , 20 cases of metastatic carcinoma from malignant ascites , 20 cases of benign gastric mucosa and 7 gastric carcinoma cell lines by two color flow cytometry with mAb CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The role of costimulation in activation for proliferation and cytokine mRNA production of peripheral blood T cells was studied by blocking CD 28 , CTLA 4 , and their ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ RESULTS : The proliferation and the production of type 1 and 2 cytokine mRNA by T cells in response to Der p as well as the control antigens TT and CA was inhibited by simultaneously masking CD 80 and CD 86 using CTLA 4 Ig , a soluble form of CTLA 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
T cell alterations in cardiac allograft recipients after B 7 ( CD 80 and CD 86 ) blockade . ^^^ CD 28 binds both the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) ligands on antigen presenting cells . ^^^ We tested the hypothesis that in vivo blockade of the CD 28 pathway via the anti CD 80 and anti CD 86 monoclonal antibodies ( mAbs ) would prolong allograft survival . ^^^ METHODS : Neonatal C57BL / 6J ( H2b ) hearts were transplanted to CBA / J ( H2k ) recipients in a heterotopic nonvascularized model , with anti CD 80 and / or anti CD 86 mAbs being administered intravenously at the time of allografting ( day 0 ) and on the following day ( day 1 ) . ^^^ RESULTS : Anti CD 80 mAb ( 29 . 8+ / 1 . 5 days ) and anti CD 86 mAb ( 30 . 8+ / 0 . 5 days ) alone significantly prolonged allograft survival compared with the isotype control ( 10 . 7+ / 0 . 4 days , P < 0 . 01 , Wilcoxon rank sum ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This is accompanied by the upregulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , CD 25 , ICAM 1 and the DC specific marker CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that CD 40 ligation enhanced ICAM 1 expression and did not upregulate HLA DR , CD 80 or CD 86 expression on IFN gamma treated keratinocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After 14 days of culture in GM CSF , TNF alpha , and IL 4 , CD34+ progenitors gave rise to a population of large , immature DC expressing CD1a and CD11b but lacking CD 14 , CD 80 , CD 83 , CD 86 , and CMRF 44 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Activation dependent transcription and expression of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were detected by reverse transcription polymerase chain reaction and flow cytometry in the absence or presence of glucocorticoids . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Candidate genes include those coding for CD 80 and CD 86 , molecules involved in antigen specific T cell recognition . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Autoimmunity without diabetes in transgenic mice expressing beta cell specific CD 86 , but not CD 80 : parameters that trigger progression to diabetes . ^^^ To define more clearly the roles of CD 80 ( RIP CD 80 ) and CD 86 ( RIP CD 86 ) in the activation of autoreactive T cells in vivo , we generated transgenic mice expressing either or both costimulatory molecules on the beta cells of the pancreas . ^^^ While RIP CD 80 mice do not show any sign of autoimmunity , at the age of 7 mo RIP CD 86 transgenic mice develop a lymphoid infiltrate with both IFN gamma and IL 4 positive cells in the vicinity of the islets ; these mice , however , never progress to diabetes . ^^^ This fundamental difference in the ability of CD 80 and CD 86 to activate self reactive T cells in vivo is , however , obliterated when the level of TCR signaling is increased by either TNF alpha or transgenic MHC class 2 expression . ^^^ These results support the suggestion that CD 80 and CD 86 mainly differ at the level of the intensity of the signals they deliver . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
While the DC phenotype , HLA DR+CD14 , was expressed by > or =90 % of the cells generated from relatives and controls , the proportion of cells that expressed CD1a and the costimulator molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) was significantly lower in IDDM relatives . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
When IL 4 consumption was blocked , it became evident that CD 80 and CD 86 both provide effective costimulatory signals for high IL 4 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we analyzed the effect of UVB irradiation on the Langerhans cells expression of several accessory molecules , namely CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In an attempt to prevent rejection , we used cyclophosphamide ( CP ) , a powerful anti B cell agent , or CTLA4Ig , an inhibitor of T cell co stimulation [ via B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) ] , either given in combination with anti CD 4 ( GK1 . 5 ) or anti CD 3 ( KT 3 ) MAb to the recipient mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 needs to be cross linked to initiate signals , yet both of its ligands , CD 80 and CD 86 , are expressed as monomers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Studies using antigen presenting cells lacking expression of both B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) show distinct requirements for B 7 molecules during priming versus restimulation of Th 2 but not Th 1 cytokine production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Because it is suggested that costimulatory molecules , such as CD 80 and CD 86 , differentially stimulate Th 1 and Th 2 cells we wished to investigate the role of these costimulatory molecules in the activation of immune suppression . ^^^ We injected UV irradiated mice with monoclonal antibodies to CD 80 and CD 86 and asked what effect , if any , this would have on UV induced immune suppression . ^^^ Anti CD 86 , but not anti CD 80 or control rat IgG , blocked UV induced immune suppression . ^^^ IL 10 was detectable in the serum of UV irradiated mice as compared with normal controls , and injecting UV irradiated mice with anti CD 86 , but not anti CD 80 or control rat IgG , blocked the secretion of IL 10 into the serum . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Ligation of CD 28 molecules expressed on the surface of human leukaemic natural killer like YT cells triggers intracellular signals leading to cytolysis of target cells expressing CD 80 or CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To study the prerequisites of functional interactions between malignant B cells and intermingled T cells in B cell non Hodgkin ' s lymphomas ( B NHL ) , we examined the expression of CD 40 , CD 80 and CD 86 and their ligands CD 40 ligand ( CD40L , CD 154 ) , CD 28 and CTLA 4 ( CD 152 ) using immunohistochemistry and confocal laser scanning microscopy . ^^^ Almost all mucosa associated lymphoid tissue ( MALT ) NHL were positive for CD 40 and CD 80 and in nine out of 14 cases were positive for CD 86 . ^^^ The majority of follicle centre cell lymphomas ( FCCL ) expressed CD 40 , but were heterogeneous in their expression of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The involvement of CD 80 and CD 86 costimulatory molecules in the induction of eosinophilia in mice infected with Nippostrongylus brasiliensis . ^^^ The costimulatory signal provided by the interaction between CD 28 and its ligands , CD 80 and CD 86 , is critical for T cell activation . ^^^ The requirement of CD 80 and CD 86 in T cell activation for eosinophilia and IgE production was examined in mice infected with the nematode parasite Nippostrongylus brasiliensis . ^^^ Combined treatment with anti CD 80 and anti CD 86 suppressed eosinophilia in the blood and the small intestine and suppressed IgE production . ^^^ However , administration of either anti CD 80 or anti CD 86 alone had little effect on eosinophilia and on the elevation of IgE levels . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules in Wegener ' s granulomatosis ( WG ) : lack of expression of CD 28 and preferential up regulation of its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on T cells . ^^^ Since the expression of costimulatory molecules has a significant impact on the cytokine profile and proliferation response of T cells , the goal of this study was to characterize the expression of costimulatory molecules ( CD 28 , CTLA 4 ( CD 152 ) , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) ) on T cells , monocytes and B cells in WG , and to correlate the findings with clinical parameters such as disease activity , extent and therapy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Regarding LC maturation in epidermal cell cultures , IL 6 and IL 10 were inhibitory for the expression of CD 86 and CD 80 in a dose dependent fashion . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The effect of immunotoxins directed against CD 80 and CD 86 on primary T cell alloresponses . ^^^ Activation of primary resting T cells requires costimulation which can be delivered by the B 7 molecules ( CD 80 and CD 86 ) expressed on activated antigen presenting cells ( APC ) . ^^^ In the present study , we examined in vitro effects of immunotoxins ( ITs ) composed of gelonin conjugated to mAbs against CD 80 or CD 86 ( alphaCD 80 IT and alphaCD 86 IT ) . ^^^ The specificity of both ITs was demonstrated using CD 80 and CD 86 transfected cell lines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Of note , CD 86 provides the initial signal to induce naive T cells to become IL 4 producers , whereas CD 80 is a more neutral differentiation signal . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The enhanced protection conferred by Flu ISCOMs in aged mice correlates with the up regulation of co stimulatory molecule , CD 86 ( B7 . 2 ) and to a lesser extent , CD 80 ( B7 . 1 ) expression on antigen presenting cells . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Parasite internalization was associated with activation manifested by upregulation of major histocompatibility complex ( MHC ) class 1 and 2 surface antigens , increased expression of costimulatory molecules ( CD 40 , CD 54 , CD 80 , and CD 86 ) , and interleukin ( IL ) 12 p 40 release within 18 h . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules , CD 80 and CD 86 ] . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Unlike ligation of CD 3 , stimulation with anti CD 28 mAb or CHO cells expressing the CD 28 ligands CD 80 or CD 86 did not lead to tyrosine phosphorylation of HS 1 in Jurkat T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To enhance a DNA vaccine ' s ability to induce CTL response in vivo , we co administered CD 80 and CD 86 expression cassettes along with HIV 1 immunogens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Such Ts cells express the CD8+CD28 phenotype and show the following characteristics : ( a ) antigen specificity and restriction by self MHC Class 1 molecules ; ( b ) limited TCR 5 beta gene usage ; ( c ) ability to inhibit antigen specific , MHC Class 2 restricted , Th proliferative responses ; and ( d ) capacity to downregulate and / or inhibit the upregulation by Th of CD 40 , CD 80 , and CD 86 molecules on APCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We also observed a more rapid loss of surface class 2 antigen expression in the ethanol , acetaldehyde , and acetate treated 43HIV cells , but no change in surface expression of CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blood derived DC up regulated MHC class 2 , CD 54 , CD 80 and CD 86 after exposure to WV vaccine , indicating their functional maturation , but were only moderately affected by subunit ( SU ) vaccines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 represent ligands for this receptor , and although they are present on professional Ag presenting cells , these molecules are absent from most tumors . ^^^ Therefore , a key question concerns the molecular basis for the costimulation of T cells by those tumor cells not expressing the CD 28 ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Further , 9 cryosections from MF lesions and 9 from SS lesions were stained for LFA , ICAM 1 , CD 40 , CD 40 ligand , CD 28 , CD 80 , CTLA 4 , CD 86 , FAS , FAS ligand , CLA and CD15s . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Harvested DCs represented yields of 4 . 1+ / 1 . 4 and 5 . 8+ / 5 . 4 % of the initial cells plated from the CD34+ cell depleted mobilized PBMCs of normal donors and cancer patients , respectively , and displayed a high level expression of CD 80 , CD 86 , HLA DR , and CD11c but not CD 14 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of MHC class 2 , CD 80 and CD 86 on EC was not affected by IL 12 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Human muscle cells express a functional costimulatory molecule distinct from B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) in vitro and in inflammatory lesions . ^^^ The B 7 family of costimulatory molecules likely includes members distinct from B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We therefore examined CD 80 and CD 86 dependence of allergen induced T cell proliferation and cytokine production in peripheral blood and bronchoalveolar lavage from atopic asthmatic subjects and controls . ^^^ Both allergen induced proliferation and IL 5 production from PBMC were inhibited by CTLA 4 Ig fusion protein and anti CD 86 , but not anti CD 80 mAbs . ^^^ When allergen specific CD4+ T cell lines from peripheral blood were examined , proliferation and cytokine production were found to be independent of CD 80 or CD 86 costimulation . ^^^ However , when cells obtained directly from the airways were examined , allergen induced proliferation of bronchoalveolar lavage T cells from atopic asthmatic subjects was inhibited by anti CD 86 but not anti CD 80 . ^^^ This was also inhibited by anti CD 86 but not anti CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show that the CD 40 induced production of IL 6 , IL 8 and TNF alpha by monocytes , and IL 12 by dendritic cells , and expression of the activation markers CD 83 , the costimulatory molecules CD 86 and CD 80 , and HLA DR antigens were all similar in patient and control cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 10 decreased the constitutive surface expression of MHC class 2 as well as of the accessory molecules CD 80 and CD 86 on LSEC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A flow cytometric analysis showed that a slight expression of CD 80 ( B 7 1 ) on monocytes was induced by NRC treatment , whereas the constitutive expressions of CD 86 ( B 7 2 ) and CD 54 ( ICAM 1 ) were unchanged . ^^^ The activation of monocytes with interferon gamma ( IFN gamma ) induced the expressions of CD 80 , CD 86 , and CD 54 under all conditions tested , but NRC treatment tended to decrease the IFN gamma induced expression of CD 54 on monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Studies of cell surface markers showed that the 2 day cultured cells had up regulated class 2 major histocompatibility complex and CD 80 , but not CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both CD 80 and CD 86 co stimulatory molecules regulate allergic pulmonary inflammation . ^^^ We examined the roles of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) in a model of allergic pulmonary inflammation and airway hyper responsiveness ( AHR ) by selectively inhibiting either CD 80 or CD 86 . ^^^ Inhibition of co stimulation by either CD 80 or CD 86 affected multiple parameters of the allergic response . ^^^ Specifically , blockade of either CD 80 or CD 86 in ovalbumin sensitized and challenged mice resulted in reduced expression of IL 2Ralpha ( CD 25 ) on CD4+ T lymphocytes , decreased airway eosinophilia , lower serum IgE production and diminished AHR . ^^^ Importantly , blockade of CD 80 and CD 86 inhibited production of IL 4 and IL 2 , and enhanced IFN gamma production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Comparative analysis of CD 80 and CD 86 on human Langerhans cells : expression and function . ^^^ Although both CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) have been recently identified in cultured human Langerhans cells ( LC ) , little is known of the role and regulatory properties of CD 80 and CD 86 on human LC . ^^^ We present here the results of a study comparing the expression and function of CD 80 and CD 86 in human LC using the T helper type 1 cytokines IL 2 and interferon gamma ( IFN ) gamma , and the T helper type 2 cytokines IL 10 , IL 4 and granulocyte / macrophage colony stimulating factor ( GM CSF ) . ^^^ Freshly isolated human LC expressed little CD 80 and CD 86 in vitro , but the expression of both molecules was rapidly induced during a 72 h incubation with cytokines and the expression of CD 86 occurred much earlier and more strongly than that of CD 80 . ^^^ The expression of both CD 80 and CD 86 was upregulated by GM CSF and downregulated by IL 10 , and the expression of CD 86 , but not that of CD 80 , was upregulated by both IL 4 and IFN gamma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They also express B 7 costimulatory molecules ( CD 80 , CD 86 ) , CD 83 , and CD1a molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells and cytokine levels ( IL 1alpha , IL 1beta , IL 2 , IL 6 , IL 8 , IL 10 , IL 13 , TNF alpha and IFN gamma ) in the afferent lymph were analyzed by cytofluorometry and ELISA . ^^^ However , no changes in the expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells were detectable . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The addition of anti CD 54 , CD 40 , CD 80 and CD 86 monoclonal antibodies to the cultures showed that the PBMC required only CD 54 and CD 86 for stimulation with C . albicans , but required CD 54 , CD 80 and CD 86 for stimulation with D . farinae . ^^^ Since many studies have suggested that T helper type 1 and T helper type 2 immune responses are different in their dependency on CD 80 or CD 86 costimulation , our present results suggest that the allergen specific T cells of AD patients are not completely shifted to a T helper type 2 subset . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
While it was found that systemic administration of mouse CTLA 4 Ig could also inhibit the progression of effector immune responses when administered shortly before or during clinical disease , these were significantly more active when delivered directly into the CNS , which probably involved an action on both CD 28 ligands , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , interleukin 12 and transforming growth factor beta mRNA expression in CSF and peripheral blood mononuclear cells from multiple sclerosis patients . ^^^ Costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are differently involved in T cell stimulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This can be provided by the accessory T cell molecule CD 28 upon binding to its respective ligands B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
VAL significantly upregulated the expression of the co stimulatory molecule B7 . 1 ( CD 80 ) on monocytes / macrophages , but not B7 . 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules CD 80 and CD 86 in the rat ; tissue distribution and expression by antigen presenting cells . ^^^ Only recently rat CD 80 and CD 86 have been cloned and monoclonal antibodies have been generated . ^^^ The target tissue of cyclosporin A induced autoimmunity , i . e . the skin and tongue , were also examined for expression of CD 80 and CD 86 . ^^^ Whereas CD 80 was hardly detected in the lymphoid tissues , CD 86 was clearly expressed by non lymphoid cells in the thymus , as well as in the secondary lymphoid organs . ^^^ On development of cyclosporin A induced autoimmunity , non professional APC , i . e . epithelial cells , started to express MHC class 2 , but not the costimulatory ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 4 + GM CSF , a combination of cytokines known to induce differentiation of dendritic cells , increased the expression of CD 40 , CD 80 and CD 86 , and decreased the expression of CD 14 on SF macrophages . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 17 promoted the maturation of DC progenitors , as evidenced by increased cell surface expression of CD11c , costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , and MHC class 2 Ag , and allostimulatory capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In order to elucidate the possibility of costimulatory molecules mediated immuno or immuno gene therapy for human hematological malignancies , we analyzed 30 hematopoietic cell lines and cells obtained from 48 patients with hematological malignancies for the expression of costimulatory molecules such as CD 80 and CD 86 . ^^^ The expression of CD 80 or CD 86 was frequent on cell lines derived from the patients with CML BC or Burkitt ' s lymphoma , while it was rare on cell lines from T ALL . ^^^ In 30 AML samples , CD 86 was positive in 15 cases ( 50 % ) , which was sharply in contrast with the finding that CD 80 was not detected in any AML samples . ^^^ We also treated seven human hematopoietic cell lines with IFN gamma , IL 12 or IL 15 and observed whether these cytokines could induce or enhance the expression of CD 40 , CD 54 , CD 58 and HLA DR as well as CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show here that 1 ) F 2 g3p was recognized with anti CTLA 4 mAb but not with anti CD 28 mAb , and 2 ) F 2 g3p bound to CD 80 but not to CD 86 . ^^^ F 2 g3p inhibited the binding of CTLA 4 to CD 80 but not to CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) expression was present on the plasma cells of only 1 of 27 samples but CD 86 ( B 7 2 ) expression within the normal range was present on the plasma cells of 14 of 27 samples . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The allergen induced secretion of IL 5 and IL 13 was inhibited by the fusion protein CTLA 4Ig , reflecting a requirement for CD 80 ( B 7 1 ) and / or CD 86 ( B 7 2 ) costimulation for the expression of the Th 2 cytokines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BACKGROUND : Administration of donor bone marrow ( BM ) derived dendritic cell ( DC ) progenitors ( DCp ) that are major histocompatibility complex ( MHC ) class II+ but costimulatory molecule ( CD 40 , CD 80 , CD 86 ) deficient can prolong mouse heart allograft survival This is associated with microchimerism and inhibition of antidonor cytotoxic T lymphocyte ( CTL ) activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Since it contains a high percentage of CD 28 and CD57+ cells , is increased in size and granularity , and is transiently expressed following in vitro stimulation of naive CD8+ T cells , we speculate that this subset mainly represents recently activated effector T cells that are able to interact with CD 80 and CD 86 ( B 7 1 and B 7 2 respectively ) negative tissue cells . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast to CD 86 expression which remained as intensive in HIV+ as in HIV lymph nodes , CD 80 staining was strongly decreased in GC of HIV+ lymph nodes but not in their extrafollicular zones . ^^^ In contrast to HIV situations where CD 80 and CD 86 was similarly expressed on B cells , the lower level of CD 80 expression in HIV+ GC may favor Th 2 T cell responses through CD 86 CD28 interactions . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 are not equivalent in their ability to induce the tyrosine phosphorylation of CD 28 . ^^^ Ligation of either CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) , two principal ligands for CD 28 , is thought to skew the immune response toward Th 1 or Th 2 differentiation . ^^^ We have examined early signal transduction pathways recruited following T cell stimulation with either CD 80 or CD 86 . ^^^ Purified human peripheral T cells or Jurkat T cells were stimulated with Chinese hamster ovary ( CHO ) cells expressing either human CD 80 ( CHO CD 80 ) or human CD 86 ( CHO CD 86 ) or with anti CD 28 monoclonal antibody ( mAb ) . ^^^ In the presence of phorbol 12 myristate 13 acetate , both CHO CD 80 and CHO CD 86 , like anti CD 28 mAb , were capable of stimulating cytokine production from both human peripheral T cells and Jurkat T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Co stimulatory factors B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) and their ligands , including CD 28 , are important for the efficient presentation and persistence of an antigen specific immune reaction . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present study we examined the expression of CD 40 , CD 40 ligand ( CD40L ) , CD 80 , CD 86 , CD 28 and cytolytic T lymphocyte associated antigen 4 ( CTLA 4 ) on lymphocytes immunohistochemically . ^^^ In vitro kinetics of the expression of CD40L and CTLA 4 by peripheral blood T cells and that of CD 80 and CD 86 by peripheral blood B cells were also investigated by flow cytometry . ^^^ Positive percentage expression of CD40L , CD 28 and CTLA 4 , and CD 40 , CD 80 and CD 86 was calculated for the number of CD3+ and CD19+ cells , respectively . ^^^ Flow cytometric analysis demonstrated that the expression of CD40L and CTLA 4 on T cells , and CD 80 and CD 86 on B cells of peripheral blood was up regulated upon activation . ^^^ While most T cells and B cells expressed CD 28 , and CD 80 and CD 86 , respectively , in gingival tissues , the expression of CD40L and CTLA 4 was lower but highly variable between specimens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The accessory effect was inhibited by pretreatment with anti CD 72 , anti B 7 ( CD 80 and CD 86 ) , and anti IL 15 monoclonal antibodies ( MoAb ) . ^^^ A 24 hour stimulation of IL 15Ralpha+ / gammac+ macrophages with IL 15 upregulated the expression of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , endotoxin treatment down regulated surface expression of constitutively expressed MHC class 2 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The role of CD 80 , CD 86 , and CTLA 4 in alloimmune responses and the induction of long term allograft survival . ^^^ Blocking the interaction of the CD 28 costimulatory receptor with its ligands , CD 80 and CD 86 , inhibits in vivo immune responses , such as allograft rejection , and in some instances induces tolerance . ^^^ Previously , we found that CTLA4Ig , which blocks the CD28 / CTLA 4 ( CD 152 ) ligands CD 80 and CD 86 , can be used to induce transplantation tolerance to vascularized allografts . ^^^ Here , we show that blockade of CD 152 using an anti CD 152 mAb at the time of transplantation prevents the induction of long term allograft survival by agents that target CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential expression and modulation of costimulatory molecules CD 80 and CD 86 on monocytes from patients with systemic lupus erythematosus . ^^^ This study was undertaken to further investigate the expression and cytokine regulation of both CD 80 and CD 86 ( B 7 2 ) on monocytes from patients with SLE . ^^^ Freshly isolated and in vitro cytokine stimulated peripheral blood mononuclear cells from 13 patients with SLE and 10 healthy subjects were analysed , cytometrically with dual fluorescence staining , to detect expression of CD 80 and CD 86 in the CD14+ monocyte population . ^^^ The results showed that , as in normal individuals , an overwhelming majority ( 95 . 62+ / 3 . 54 % ) of monocytes from patients with SLE expressed the CD 86 molecule , but only a few monocytes ( 5 . 54+ / 4 . 36 % ) had detectable CD 80 expression . ^^^ The effects of interleukin 10 ( IL 10 ) on the expression of CD 80 and CD 86 on monocytes from patients with SLE and normal controls were similar . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Increased expression of CD 80 and CD 86 in in vitro infected CD3+ cells producing cytoplasmic HIV type 1 p 24 . ^^^ A striking proportion of p24+ cells expressed CD 80 or CD 86 , antigens not usually expressed by CD3+ lymphocytes . ^^^ CD 28 expression was generally comparable between CD3+ cells that did and did not express CD 80 or CD 86 . ^^^ Using this approach , we found an association between productive infection and increased expression of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The yield as well as the pattern and intensity of Ag ( HLA DR , CD 40 , CD 54 , CD 80 , and CD 86 ) expression were equivalent on DCs from BM or PB of MM patients versus normal donors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 costimulatory molecules on circulating T cells of HIV infected individuals . ^^^ The expression of CD 80 and CD 86 costimulatory molecules , typical for antigen presenting cells ( APC ) , was measured on circulating T cells of 20 HIV infected individuals and of 11 HIV negative healthy controls . ^^^ The CD 80 and CD 86 molecules were present on both circulating T subsets of HIV infected individuals ( mean of CD 80 expression within CD4+ T cells [ CD80 / CD4 ] : 5 . 0 % ; and CD86 / CD4 : 2 . 6 % ; CD80 / CD8 4 . 1 % and CD86 / CD8 : 2 . 7 % ) and were associated with HLA DR expression . ^^^ Some CD 80 and CD 86 expression was also found in normal controls , and only the expression of CD 86 was significantly ( P < 0 . 05 ) increased on CD 4 + and CD 8 + T cells of HIV infected individuals . ^^^ This suggests that during HIV infection T cells themselves develop an antigen presenting phenotype by upregulating expression of HLA DR , CD 86 and CD 80 molecules . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both cell lines express the same light ( kappa ) and heavy chains ( mu , delta ) as the fresh parental B CLL cells at the same high intensity , share the expression of mature B cell markers ( CD 19 , CD 20 , CD 21 , CD 22 ) , differ in the expression of CD 23 and FMC 7 , are CD11a+ , CD18+ , CD44+ , CD49d+ , CD54+ and express at high levels both CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment with BPD MA and light reduced DC levels of major histocompatibility ( MHC ) Class 1 and 2 antigens , intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , the costimulatory B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules , leucocyte common antigen CD 45 , the apoptosis regulating Fas ( CD 95 ) receptor and the integrin CD11c . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Rather , CD 8 ( + ) T cell apoptosis was caused by antigen presenting cells because removal of monocytes or addition of antibodies to CD 80 and CD 86 reduced apoptosis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 80 and CD 86 on peripheral blood T lymphocytes in patients with systemic lupus erythematosus . ^^^ CD 80 and CD 86 were detected in high amounts on circulating T cells in the peripheral blood of some patients with systemic lupus erythematosus ( SLE ) , using flow cytometry and monoclonal antibodies . ^^^ Patients with other connective tissue diseases did not have a high percentage of T cells expressing CD 80 or CD 86 in their peripheral blood . ^^^ CD 80 was expressed mainly on CD 4 T cells , whereas CD 86 was expressed on CD 8 T cells , and these two populations were associated with particular clinical features . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OBJECTIVE : To examine whether rheumatoid synovial fluid ( SF ) inhibits dendritic cell ( DC ) expression of the CD 80 and CD 86 costimulator molecules and contributes to SF T lymphocyte hyporesponsiveness . ^^^ In the presence of SF , DC clustered with autologous T lymphocytes showed decreased CD 80 and CD 86 expression , and variable CD80 / CD86 decreases were observed on DC clustered with allogeneic T lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Functional expression of CD 80 and CD 86 allows immunogenicity of malignant B cells from non Hodgkin ' s lymphomas . ^^^ Among the 70 samples of malignant B cells included , four patterns of expression of the costimulatory molecules CD 80 and CD 86 were distinguished ( + / + , + / , / + and / ) . ^^^ In two thirds of the cases , CD 80 , CD 86 , or both were expressed . ^^^ Regardless of the level of expression of CD 80 and CD 86 , significant proliferation was induced in the responder cells . ^^^ The addition of monoclonal antibodies directed against CD 80 and CD 86 at the beginning of MLR almost completely inhibited this proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Scurfy T cells also exhibit strong up regulation of cell surface Ags indicative of in vivo activation , including CD 69 , CD 25 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They expressed markers related to DCs ( CD1a , CD11c , CD 32 and CD 83 ) , costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , human leucocyte antigen ( HLA ) class 1 and 2 molecules and inducible nitric oxide ( NO ) synthase ( NOS 2 ) , and lacked lymphocyte and monocyte markers ( CD 3 , CD 19 , CD 20 , CD 56 and CD 14 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CBDC expressed CD 4 , CD11a , CD 18 , CD45RA , CD 50 , CD 54 , and CD 123 , but showed no expression of CD1a , CD11c , CD 33 , CD 40 , CD45R0 , CD 80 , CD 83 , and CD 86 and only limited expression of CD 58 , CD 102 , and CD 116 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both molecules bind to the same ligands , B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) but have antagonistic functions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CD1a+ cells reacted with antibodies against protein S 100 , HLA DR , the Lag antigen , CD 4 , CD11a , CD11b , CD 18 , CD 25 , CD 40 , CD 54 , CD 80 and CD 86 . ^^^ Both allogenic and antigen specific T cell proliferation stimulated by antigen presenting lymph cells were strongly inhibited by adding anti CD 80 and anti CD 86 antibodies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To determine whether antilymphocyte Abs to T cell costimulatory molecules are generated in patients with autoimmune diseases and , if they exist , to clarify the mechanism of their production and pathological roles , we investigated the presence of autoantibodies to CTLA 4 ( CD 152 ) , CD 28 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) in serum samples obtained from patients with various autoimmune diseases and from normal subjects using recombinant fusion proteins . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In accordance with the characteristics of in vivo LC , we show that LC obtained from human monocytes in vitro in the presence of TGF beta 1 1 ) express almost exclusively intracellular class 2 Ags , low CD 80 , and no CD 83 and CD 86 Ags and 2 ) down regulate TNF RI ( p 55 ) and do not produce IL 10 after stimulation , in contrast to dermal dendritic cells and monocyte derived dendritic cells . ^^^ Surprisingly , while LC exhibit E cadherin down regulation upon exposure to TNF alpha and IL 1 , TGF beta 1 prevents the final LC maturation in response to TNF alpha , IL 1 , and LPS with respect to Class 2 CD 80 , CD 86 , and CD 83 Ag expression , loss of FITC dextran uptake , production of IL 12 , and Ag presentation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cells expressing CD 4 , CD 8 , CD 18 , CD49d / CD29 , CD 44 , CD 54 , CD 80 , CD 86 , CD 106 , CD11b / CD18 or DNA breaks were stained in the pancreases of female or male scid / scid mice after adoptive transfer of lymphocytes from older than 12 week female nonobese diabetic ( NOD ) or Balb / c mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Monocyte / macrophage markers included CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 54 , CD 71 , CD 80 and CD 86 and HLA class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DNA immunization with the myelin minigene for PLP altered expression of B7 . 1 ( CD 80 ) , and B7 . 2 ( CD 86 ) on APCs in the spleen . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
T cell activation requires binding of the T cell receptor to the major histocompatibility molecule peptide complex in the presence of adhesion and / or costimulatory molecules such as B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , ICAM 1 ( CD 54 ) , and LFA 3 [ corrected ] . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interaction of CD 28 with CD 80 or CD 86 molecules on APC initiates a costimulatory or second signal within the T cell which augments and sustains T cell activation initiated through the TCR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Bladder cancer immunogenicity : expression of CD 80 and CD 86 is insufficient to allow primary CD4+ T cell activation in vitro . ^^^ This in vitro study examined the potential of such a strategy by transfection of the prototypical TCC line J 82 with the important costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ Transfected J 82 cells expressed CD 80 or CD 86 at levels comparable to an antigen presenting B cell line . ^^^ Furthermore , functional surface expression of CD 80 and CD 86 was demonstrated in a mitogen dependent assay of costimulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Epidermal cells were prepared from these earlobes 24 h later , and the levels of MHC class 2 , CD 54 , CD 80 , and CD 86 on these cells were analyzed by flow cytometry . ^^^ As compared with untreated earlobes , the levels of MHC class 2 and CD 86 on LC were markedly augmented and those of CD 54 and CD 80 were slightly elevated in earlobes treated with TCSA / UVA . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Only a few DC in the uveal tract were very weakly reactive for mAbs which recognise B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , beta 2 integrin ( mAb N 418 ) , and multivesicular bodies associated with antigen presentation ( mAb M 342 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : We investigated surface expression of the costimulatory molecules CD 58 ( LFA 3 ) , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) by using flow cytometry , confocal microscopy , and vectorial biotinylation . ^^^ Antibodies specific for CD 58 , CD 80 , and CD 86 were used in blocking experiments to assess the role of these molecules in providing a costimulatory signal to CD 4 ( + ) T cells by IECs . ^^^ RESULTS : CD 58 , but not CD 80 or CD 86 , was observed to be expressed constitutively on both native IECs and in the IEC lines T 84 and HT 29 . ^^^ Antibodies against CD 58 , but not CD 80 or CD 86 , inhibited the stimulation of CD 4 ( + ) T cell proliferation mediated by IECs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast to results described with B type chronic lymphocytic leukemia , also characterized by high levels of circulating TNF , none of the tested samples showed a regulation of CD 80 , CD 86 , CD 27 and CD 70 in response to TNF . ^^^ After culture with human CD40L transfected L cells , an up regulation of CD 80 , CD 86 and CD 70 expression was observed , while TNF addition in this model did not influence these changes . ^^^ Taken together , this data demonstrates that in vitro expression of costimulatory molecules such as CD 80 , CD 86 , CD 27 and CD 70 , which are implicated in the anti tumoral response , can be regulated by CD 40 ligand but not by TNF . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have studied the immune response of the host testis against experimental testicular teratoma in mouse by localizing adhesion molecules ( CD 106 , CD 54 , CD49d / CD29 , CD 44 , CD 18 , CD 8 and CD 4 ) , cytokines ( IL 2 , IL 4 , IL 6 , IL 10 and IL 12 ) , T cell costimulators ( CD 80 , CD 86 ) and the lipid antigen presenting molecule CD1d in the testis of 129 / SvJ mice with and without experimental testicular teratoma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of LFA 1 , ICAM 1 , CD 80 and CD 86 molecules in lupus patients : implication for immunotherapy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The supernatant factor also exhibits a marked inhibitory effect on the expression of the costimulatory molecules , CD 80 and CD 86 , by APC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have developed several in vivo models to study allergen specific T cell and B cell function and their relevance to allergic airway hyperresponsiveness ( AHR ) , focusing on the role of the costimulatory molecules CD 80 and CD 86 . ^^^ Treatment of mice with anti CD 86 , but not anti CD 80 , significantly inhibited increased serum levels of ovalbumin ( OA ) specific IgE and IgG 1 , airway eosinophilia , and AHR both after 10 d of OA aerosol exposure ( in the absence of adjuvant ) and after intraperitoneal sensitization followed by repeated airway challenges . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
As the B7 / CD28 costimulatory pathway has been shown to influence the differentiation of Th cell subsets , we investigated the contribution of the B 7 molecules CD 80 and CD 86 to Th1 / Th2 cytokine and immunoglobulin isotype profiles and to the development of a protective immune response to malaria in NIH mice infected with Plasmodium chabaudi . ^^^ Moreover , combined CD80 / CD86 blockade by using anti CD 80 and anti CD 86 monoclonal antibodies raised IFN gamma production over that seen with CD 86 blockade alone , with augmentation of this Th 1 associated cytokine reducing levels of peak primary parasitaemia . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Since these two receptors use the same ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , the co ordinate timing of CD 28 and CTLA 4 expression has a major impact on the regulation of immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The unique functional characteristics of microvascular LSECs together with a unique phenotype ( CD 4 ( + ) , CD11b+ , CD11c+ , CD 80 ( + ) , CD 86 ( + ) ) make these cells different from macrovascular endothelial cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To verify whether alveolar macrophages ( AM ) of patients with hypersensitivity pneumonitis ( HP ) increase their antigen presenting capacity by upregulating the expression of B 7 costimulatory molecules ( CD 80 , CD 86 ) , and whether a viral infection enhances this expression whereas cigarette smoking abrogates it , we performed bronchoalveolar lavage ( BAL ) on 18 patients with HP ; 10 asymptomatic , virus exposed subjects ( AS ) ; 18 nonsmokers ; and 12 smokers . ^^^ Expression of CD 80 and CD 86 on AM , and of CD 28 and CTLA 4 on T cells , was evaluated . ^^^ In vitro virus infection upregulated CD 80 and CD 86 expression in AM of normal nonsmoking subjects but not on those of smokers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Monocyte derived DC transiently incubated with the anti CD 43 mAb , MEM 59 , or with F ( ab ' ) 2 fragments , but not with monovalent Fab fragments or control IgG , 24 h later showed increased levels of membrane HLA DR , CD 54 , CD 40 , CD 80 , CD 86 , and CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression and contribution of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in the early immune response to Leishmania major infection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Compared with mature recirculating follicular B cells , they express several characteristics of previous antigenic experience , including higher levels of B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) when freshly isolated and following in vitro stimulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , no up regulation of CD 80 , CD 86 , CD45RC and OX 40 ( CD 134 ) was observed on T cells during T T presentation or subsequent antigenic restimulation of anergic T cells in the presence of professional APC , whereas increased expression of these molecules was observed during professional APC T presentation of non anergic T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OBJECTIVE : We studied whether this difference may be correlated to increased expression of membrane costimulatory molecules , such as the B 7 molecules ( CD 80 and CD 86 ) . ^^^ RESULTS : Allergic individuals had a significantly higher proportion of AMs expressing the CD 80 molecule than control subjects ( 28 . 5 % + / 14 . 8 % vs 1 . 4 % + / 1 . 2 % ; P < . 001 ) , whereas no difference was observed in CD 86 expression ( 2 . 0 % + / 2 . 3 % vs 1 . 1 % + / 0 . 6 ; P > . 1 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
MP prevented LPS induced DC maturation as assessed by the inhibition of CD 86 , CD 80 and CD 54 up regulation , CD 83 induction and production of TNF alpha , IL 6 , and IL 12 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , neither CD 80 nor CD 86 nor CD 102 was found on the surface , and these costimulatory molecules could not be induced by any cytokines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Enhanced expression of CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 and their ligands CD 28 and CD 154 in fulminant hepatic failure . ^^^ To define a possible role for changes in the regulation of antigen presentation in fulminant hepatic failure ( FHF ) , we studied the expression of co stimulatory molecules CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 along with their ligands CD 28 and CD 154 . ^^^ In FHF , immunoreactivity for CD 80 and CD 86 was observed on significantly higher numbers of cells , including SECs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) costimulatory molecules in tumor cells has proven to be an efficient way to enhance their immunogenicity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory molecules CD 80 and / or CD 86 by a Kaposi ' s sarcoma tumor cell line induces differential T cell activation and proliferation . ^^^ Besides the first antigen specific signal , the second costimulatory signal involves CD 80 and CD 86 expressed by the antigen presenting cell . ^^^ We previously observed that tumor cells in Kaposi ' s sarcoma ( a common AIDS related cutaneous neoplasm ) completely lack both CD 80 and CD 86 , and these tumor cells fail to stimulate T cell proliferation . ^^^ Tumor cells that were either singly positive for either CD 80 or CD 86 , as well as a double positive cell line , were examined for their ability to induce T cell activation , T cell proliferation , and cytokine production profiles . ^^^ Compared to the parental double negative tumor cell line , the CD 80 positive cells , but not the CD 86 positive tumor cells , induced significant T cell activation and proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Two colour flow cytometry revealed a similar increase in donor derived H 2Kb+ / I Ab+ cells , and in the incidence of donor leucocytes expressing CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The interaction between the T cell surface molecules CD 28 and costimulatory molecules of antigen presenting cells ( CD 80 or CD 86 ) is the most important point of the T helper cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vitro derived DC were infected with BCG , which induced their maturation , as shown by the increased expression of MHC class 2 antigens , CD 80 and CD 86 co stimulatory molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
First , we found that the addition of IFN beta at the initiation of the culture did not modify DC morphology but caused a reproducible and statistically significant upregulation of HLA DR , CD 86 , and CD 80 surface expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These mutations in CTLA 4 ablated binding to its natural ligands CD 80 and CD 86 , whereas binding to a conformation dependent anti CTLA 4 monoclonal antibody showed that the 5 domain framework remained correctly folded . ^^^ CTLA 4 displayed on phage bound specifically to immobilized CD 80 Ig and CD 86 Ig and in one step panning enriched 5 , 000 to 2 , 600 fold respectively over wild type phage . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we examined whether SCC that undergo transformation and development in the absence of specific immunity exhibit differences in tumorigenicity that relate to differences in expression of CD 80 , CD 86 or MHC class 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) antigens on house dust mite specific T cells in atopic disease function through T T cell interactions . ^^^ BACKGROUND : CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) play an important role in antigen presentation to effector cells . ^^^ However , the functional role of CD 80 and CD 86 expressed on allergen specific T cells in atopic diseases has not yet been clarified . ^^^ OBJECTIVE : We sought to determine the functional role of CD 80 and CD 86 expressed on allergen specific T cells in atopic diseases . ^^^ METHODS : We assayed the expression of CD 80 and CD 86 on allergen specific T cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae crude ( Der f c ) antigen , 1 of the major allergens causing house dust mite allergy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We determined the in situ phenotype of mature CD83+ TiDCs using monoclonal antibodies to known activation molecules ( CD 86 [ B7 . 2 ] , CD 80 [ B7 . 1 ] , CD 40 , CD 54 , CD1a and HLA DR ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The ligands or counterreceptors for these two proteins are the B 7 family members , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , anti CD 40 mAb was found to enhance the expression of the B 7 2 ( CD 86 ) and , to a lesser extent , the B 7 1 ( CD 80 ) costimulatory molecules on B220+ cells ( i . e . , B cells ) , and B 7 2 and , to a lesser extent , B 7 1 molecules played an important role in the potentiating effect of anti CD 40 mAb for CTL generation by tumor bearer splenic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendritic cells generated in mice treated with Flt3L alone or in combination with CD40L were equally potent in stimulating allogeneic T cells and expressed similar levels of MHC class 2 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Collectively , these results demonstrate the importance of costimulation for naive CD8+ T cell activation , suggest that CD 80 and CD 86 can mediate opposing effects , possibly due to differential interaction with CD 152 and CD 28 , and indicate differences in the sensitivity of immature vs mature CD8+ T cells to the induction of nonresponsiveness following costimulation deficient Ag presentation . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Significant differences in surface antigen expression DC vs M were recognizable for MHC class 2 , CD 86 , CD 80 , CD1a , CD 40 and ICAM 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We sought to enhance the protective immune response induced during Mycobacterium bovis BCG infection by administering an antibody that blocks the interaction of CTLA 4 with its ligands , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These are provided by costimulatory molecules , including B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) and intercellular adhesive molecule 1 ( ICAM 1 ; CD 54 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , the expression of CD 80 and CD 86 , which both decreased in the stress burdened mice , was restored to the normal level by TJ 41 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although the promyelocytic leukemia line HL 60 was refractory to cytokine differentiation , CI treatment induced near uniform overnight expression of CD 83 , CD 80 ( B7 . 1 ) , and CD 86 ( B 7 . 2 ) , as well as additional characteristics of mature DC . ^^^ In parallel studies , CD 34 ( pos ) cells cultured from normal donor bone marrow developed marked DC like morphology after overnight treatment with either rhCD40L or CI , but only CI simultaneously induced upregulation of CD 83 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B7 . 2 ( CD 86 ) but not B7 . 1 ( CD 80 ) costimulation is required for the induction of low dose oral tolerance . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , expressions of S 100 , CD 25 , CD 68 , CD 80 , CD 86 , and Fas ligand were identified on these cells by immunocytochemical staining . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : Forty six patients with MS and 29 healthy individuals were analyzed by direct 2 color immunofluorescence flow cytometry for expression of CD 80 , CD 86 , CD 28 and CTLA 4 on T and B cells and monocytes . ^^^ Marked increase in the expression of CD 80 and CD 86 was seen on both CD4+ and CD8+ T cells from an MS patient with rapidly progressing disease . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recombinant IL 16 was found to up regulate CD 25 and CD 80 but to down regulate CD 4 and CD 86 surface expression in MDM cultures . ^^^ However , no change could be observed on the level of CD 4 , CD 80 and CD 86 expression in IL 16 stimulated MDDC , although a significant up regulation of CD 25 and CD 83 was consistently detected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here we show that peripheral blood eosinophils from hypereosinophilic patients could express membrane CD 86 but not CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , human ECs may be limited in their ability to effectively activate CD 4 ( + ) T cells , because they do not express members of the B 7 family ( CD 80 and CD 86 ) of costimulatory molecules . ^^^ By reverse transcriptase polymerase chain reaction , Western blot , and FACS ( ( R ) ) analysis , we could not detect the expression of CD 80 and CD 86 on activated ECs and found minimal expression on purified CD 4 ( + ) T cells . ^^^ In contrast , both CD 80 and CD 86 were expressed in allogeneic CD 4 ( + ) T cell EC cocultures . ^^^ Expression of CD 86 peaked at early times between 12 and 24 h after coculture , whereas CD 80 was not expressed until 72 h . ^^^ Addition of anti CD 86 but not anti CD 80 monoclonal antibodies to cocultures inhibited IL 2 production and the proliferation of CD 4 ( + ) T cells to allogeneic donor human umbilical vein ECs ( HUVECs ) , as well as to skin and lung microvascular ECs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , traf 5 ( / ) B cells showed defects in proliferation and up regulation of various surface molecules , including CD 23 , CD 54 , CD 80 , CD 86 , and Fas in response to CD 40 stimulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Inhibition of the interaction of T cells with CD 86 , but not CD 80 , at the time of exposure to intranasal Ag prevented the development of unresponsiveness , while selective blockade of CTLA 4 had no effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The accessory function of antigen presenting cells depends on the presence of a number of costimulatory molecules , including members of the B 7 family ( CD 80 and CD 86 ) and the CD 5 coligand CD 72 . ^^^ Although normal alveolar macrophages ( AMs ) did not bear or bore low levels of costimulatory molecules , AMs from sarcoid patients with CD 4 T cell alveolitis upmodulated CD 80 , CD 86 , and CD 72 and expressed high levels of interleukin ( IL ) 15 ; lymphocytes accounting for T cell alveolitis expressed Th 1 type cytokines [ interferon ( IFN ) gamma and / or IL 2 ] and bore high levels of CD 5 and CD 28 but not of CD 152 molecules . ^^^ In vitro stimulation of AMs with Th 1 related cytokines ( IL 15 and IFN gamma ) upregulated the expression of CD 80 and CD 86 molecules . ^^^ The hypothesis that CD 80 and CD 86 molecules regulate the sarcoid T cell response was confirmed by the evidence that AMs induced a strong proliferation of T cells that was inhibited by pretreatment with CD 80 and CD 86 monoclonal antibodies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , in vitro propagated DC were transduced using an adenoviral ( Ad ) vector to express the gene encoding cytotoxic T lymphocyte antigen 4 immunoglobulin ( CTLA4lg ) , which blocks interaction of CD 80 and CD 86 on DC with CD 28 on T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In particular , S . gordonii dose dependently up regulated expression of membrane molecules ( MHC 1 and 2 , CD 80 , CD 86 , CD 54 , CD 40 , CD 83 ) and reduced both phagocytic and endocytic activities . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , CD 40 ligation , but not TNF alpha or lipopolysaccharide treatment , of activated , migratory DC that had migrated from skin explants resulted in a 2 . 5 fold increase of surface expression of FK without significant alterations of expression of CD 80 , CD 86 , CD 54 or MHC class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : CD 40 CD40L interactions induced the expression of the adhesion molecules VCAM 1 and E selectin and the costimulatory molecule CD 80 but not CD 86 ( B 7 2 ) on the MECs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The NEC and AEC also expressed the costimulatory molecules CD 80 and CD 86 as determined with flow cytometry , the reverse transcription polymerase chain reaction for RNA , and immunohistochemistry , and T cell proliferation could be blocked by treating NEC and AEC with anti CD 80 and anti CD 86 antibodies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The aim of this study was to demonstrate expression of costimulatory molecules CD 86 and CD 80 in human colonic epithelial cells and assess their functional roles in the activation of T cells in inflamed colonic mucosa . ^^^ METHODS : CD 86 and CD 80 expression was assessed by immunohistochemistry of colonic mucosa , reverse transcription polymerase chain reaction , and flow cytometric analysis of isolated colonic epithelial cells and cell lines . ^^^ RESULTS : CD 86 and CD 80 messenger RNA was detected in isolated colonic epithelial cells from normal and inflamed mucosa of patients with ulcerative colitis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment of purified CD 33 ( + ) CML cells from 15 patients with calcium ionophore ( CI ) consistently resulted in de novo expression of the costimulatory molecules CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , CD 40 and the DC specific activation marker CD 83 , as well as marked up regulation of MHC class 1 and 2 molecules and the adhesion molecule CD 54 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Tumor cell lines also expressed epidermal growth factor ( EGF ) receptor , MHC class 1 , and CD 95 ( Fas ) but did not uniformly express other important lymphoid regulatory molecules such as CD 80 , CD 86 , or interleukin ( IL ) 2 receptor components . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Human T cell response to L 14 pig cell line transfected with human ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Human fibroblasts transduced with CD 80 or CD 86 efficiently trans costimulate CD4+ and CD8+ T lymphocytes in HLA restricted reactions : implications for immune augmentation cancer therapy and autoimmunity . ^^^ We have evaluated the efficiency of CD 80 and CD 86 mediated trans costimulation in the activation of human CD8+ and CD4+ T lymphocytes in MHC class 1 and class 2 restricted lymphoproliferation reactions . ^^^ Our studies demonstrate that the efficiency of CD 80 or CD 86 mediated trans costimulation of purified human CD8+ and CD4+ T lymphocytes is comparable to cis costimulation under defined conditions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The monocytic cells did not account for this difference , as CD 80 and CD 86 expressions were similar , and coculturing PB T cells with alveolar macrophages resulted in no difference in IFN gamma production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The DC phenotype was defined as upmodulation of the costimulatory molecules CD 80 and CD 86 and the expression of CD1a or CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They expressed a set of DC associated markers , such as MHC class 2 , CD1a , CD 4 , CD11a , CD 40 , CD 58 , CD 80 , CD 83 , CD 86 , and CXCR 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In parallel , CT induced up regulation of CD 80 and CD 86 on these Flt3L expanded DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Whereas the virus infected T cells expressed higher levels of HLA DR , CD 25 , CD 80 , and CD 86 Ags than apoptosis resistant PHA blasts , the T cell apoptosis was enhanced by addition of exogenous IL 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 ( CD 152 ) , a receptor for the B 7 costimulatory molecules ( CD 80 and CD 86 ) , is considered a fundamental regulator of T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both proteins are ligands for the costimulatory molecules CD 80 and CD 86 expressed by activated B cells , macrophages , and dendritic cells . ^^^ A fusion protein comprising the CTLA 4 extracellular domain joined to a human immunoglobulin heavy chain constant region ( CTLA4Ig ) binds CD 80 and CD 86 with high affinity and inhibits CD80 / CD86 dependent immune responses in vitro and in vivo . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 40 , CD 80 , and CD 86 antigens were constitutively expressed on 3 . 2 % + / 4 . 5 % , 0 % , and 1 . 8 % + / 1 . 2 % CD 34 ( + ) blood cells , respectively . ^^^ After incubation for 24 hours with TNF alpha CD 34 ( + ) CD 40 ( + ) blood cells expressed only myeloid markers and contained less than 5 % CD 86 ( + ) and CD 80 ( + ) cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 interactions with either CD 80 or CD 86 are sufficient to induce allergic airway inflammation in mice . ^^^ In the present study , the importance of CD 28 as well as the individual roles of CD 80 and CD 86 were examined in this system using wild type and CD 28 knockout ( KO ) mice . ^^^ We next determined by polymerase chain reaction and flow cytometry that CD 80 and CD 86 molecules are constitutively expressed in the naive murine lung and on eosinophils in the allergic lung , suggesting a potential important role for both ligands in the development of asthma . ^^^ Combined anti CD80 / anti CD 86 treatment throughout the antigen challenge period fully blocked the development of allergic airways , whereas a partial reduction was observed in mice treated with either anti CD 80 or anti CD 86 antibody alone . ^^^ Therefore , signaling through either CD 80 or CD 86 is sufficient to generate a partial local allergic response , whereas CD 86 costimulation is essential to induce systemic allergic ( IgE ) reactions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Antibodies against muscarinic M 3 receptor and expression of costimulatory molecules ( CD 80 and CD 86 ) by ductal epithelial cells may play a role in pathogenesis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NK cell triggering by the human costimulatory molecules CD 80 and CD 86 . ^^^ Recently , it has been described that murine NK cells are triggered by the costimulatory molecules CD 80 , CD 86 , and CD 40 . ^^^ Using NK cell lines derived from PBMC as effectors , we demonstrate that the human CD 80 and CD 86 gene products can function as triggering molecules for NK cell mediated cytotoxicity . ^^^ Expression of human CD 80 or CD 86 molecules in murine B16 . ^^^ Triggering of human NK cells by CD 80 and CD 86 appeared to be independent of CD 28 and CTLA 4 , at least as determined by the reagents used in the present study , because the expression of these molecules could not be detected on the NK cell lines by either flow cytometry or in redirected lysis assays . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Among these accessory molecules , interactions between CD28 / CTLA 4 with B 7 family members ( CD 80 and CD 86 ) and CD 40 with CD 40 ligand ( CD40L ) play a decisive role in regulating the progression of balanced immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expression in multiple sclerosis patients : clinical subtype specific variation in peripheral monocytes and B cells and lack of modulation by high dose methylprednisolone . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
As the co stimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) play an integral role in the activation of T cells by antigen presenting cells ( APC ) we investigated the surface expression of B 7 1 and B 7 2 on colonic macrophages from normal and IBD mucosa . ^^^ RESULTS : The expression of B 7 1 ( CD 80 ) ( 9 . 2 % + / 4 . 2 % ) and B 7 2 ( CD 86 ) ( 15 . 1 % + / 7 . 3 % ) was low on colonic macrophages from normal mucosa , indicating only a low antigen presenting potential . ^^^ However , on macrophages from IBD colon there was a significant increase in the expression of co stimulatory molecules ( CD 80 , 33 . 8 % + / 8 . 9 % , P = 0 . 00005 vs . control ; CD 86 , 39 . 9 % + / 8 . 8 % , P = 0 . 00002 ) . ^^^ There was no significant difference between CD and UC in the expression of CD 80 ( CD , 31 . 3 % + / 6 . 7 % ; UC , 34 . 4 % + / 13 . 3 % ) and CD 86 ( CD , 41 . 9 % + / 3 . 8 % ; UC , 35 . 6 % + / 13 . 8 % ) . ^^^ CONCLUSION : Colonic macrophages from normal mucosa rarely express the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) as activation markers that are present at low density on the cell surface was analyzed in comparison to CD 22 ( B cells ) and CD 4 ( T cell subset ) , that show high staining intensities . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The role of CD 80 and CD 86 in enhancing CD 8 ( + ) cell suppression of HIV replication . ^^^ In the present study CD 8 ( + ) cells exposed to purified forms of both the CD 80 and the CD 86 molecules during stimulation with anti CD 3 antibodies ( Ab ) had a greater ability to suppress HIV replication than CD 8 ( + ) cells exposed to anti CD 3 Ab alone . ^^^ The addition of anti CD 86 blocking Ab , but not anti CD 80 blocking Ab , to Mphi decreased their ability to enhance CD 8 ( + ) cell suppression of HIV replication . ^^^ Moreover , anti CD 86 Ab and not anti CD 80 Ab blocked the production of IL 2 by CD 8 ( + ) cells stimulated in the presence of Mphi . ^^^ The difference in use of CD 86 over CD 80 molecules on Mphi by CD 8 ( + ) cells mediating the antiviral suppressing activity most likely results from a higher number of Mphi expressing the CD 86 molecule compared with the CD 80 molecule . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
P ExA also inhibited IFN gamma induced cellular events : ( 1 ) expression of costimulatory molecules on monocytes ( CD 80 , CD 86 , ICAM 1 , HLA DR ) ; ( 2 ) cytotoxic activity of NK cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dual flow cytometry analysis performed weekly demonstrated increasing co expression of CD1a or CD 83 with HLA DR , CD 80 , CD 86 , IL 2R alpha , beta , and gamma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These are : ( 1 ) low expression of the costimulatory molecules CD 40 , CD 80 , and CD 86 ; ( 2 ) poor expression of the differentiation marker CD 83 and no CD1a ; and ( 3 ) good capacity to incorporate dextran . ^^^ Although lung DC express low levels of CD 80 and CD 86 , the important role of these costimulatory molecules in inducing high MLR was demonstrated by using blocking antibodies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , the minority ( 10 30 % ) of DC clustering with T lymphocytes showed antigen specific up regulation of the CD 40 , CD 80 and CD 86 costimulator molecules , whereas the non clustered DC ( 70 90 % ) had less up regulation than control DC cultured alone and did not respond to antigen specific triggering . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , expression of the co stimulatory ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , as well as the receptors CD 28 and CTLA 4 , were quantitated in central nervous system ( CNS ) tissues from mice at various stages of EAE . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Glycosyl phosphatidylinositol ( GPI ) modified variants of the murine costimulators B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , designated B 7 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
UVB stimulated the maturation of the former LC subset as demonstrated by enhanced up regulation of CD 80 , CD 86 , CD 54 , CD 40 , and CD 83 and reduced CD1a expression in comparison with unirradiated controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Screening of various soluble factors revealed that IL 4 induces phenotypic maturation of XS 52 ( as evaluated by enhanced expression of class 2 , CD 40 , CD 80 , CD 86 , CD11c , and loss of expression of CD 14 ) in a time dependent manner . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Addition of TNF alpha or LPS induced both normal and CMML Mo DC to express prominent dendritic processes , the CMRF44+ and CD83+ antigens and high levels of HLA DR , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Peripheral blood antigen presenting cells from African Americans exhibit increased CD 80 and CD 86 expression . ^^^ The expression of both B 7 costimulatory molecules was significantly higher on the cells from AA subjects compared with cells from CS subjects ( CD 80 , P < 0 . 05 ; CD 86 , P < 0 . 05 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After 7 days , when returned to medium containing human serum without cytokines , some monocyte derived dendritic cells ( MDDC ) became adherent , but retained the costimulatory markers CD 80 and CD 86 and continued to express CD 83 and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Characteristic of dendritic cell maturation , R 848 treatment induces cell surface expression of CD 83 and increases cell surface expression of CD 80 , CD 86 , CD 40 , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We now show that infection by rAd enhances the immunostimulatory capacity of immature human monocyte derived DC through the upregulation of the costimulatory molecules CD 80 , CD 86 , and CD 40 and the major histocompatibility complex class 1 and 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
FACS analysis of CD2+ and CD 2 monocytes revealed no significant difference in the expression of adhesion molecules ( CD11a / b / c ) , class 2 Ags ( HLA DR , DQ , DP ) , myeloid Ags ( CD 13 , CD 14 , CD 33 ) , or costimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells were characterized by the production of IL 10 and TGF beta 2 , but not IL 12 , IL 1alpha , or TNF alpha , and they expressed CD 14 , CD 16 , and CD 54 , but not HLA DR , CD 80 , CD 86 , CD11a , CD11b , or CD 25 cell surface Ags . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Essential role for both CD 80 and CD 86 costimulation , but not CD 40 interactions , in allergen induced Th 2 cytokine production from asthmatic bronchial tissue : role for alphabeta , but not gammadelta , T cells . ^^^ CD 80 and CD 86 interact with CD 28 and deliver costimulatory signals required for T cell activation . ^^^ Allergen induced IL 5 and IL 13 production by the asthmatic tissue was inhibited by anti CD 80 and , to a lesser extent , by anti CD 86 mAbs . ^^^ In contrast , the production of these cytokines by PBMCs was not affected by mAbs to CD 80 , was inhibited by anti CD 86 , and was strongly attenuated in the presence of both Abs . ^^^ Collectively , these findings suggest that allergen specific alphabeta T cells are resident in asthmatic bronchial tissue and demonstrate that costimulation by both CD 80 and CD 86 is essential for allergen induced cytokine production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In combination with Tumor necrosis factor alpha , ATP increased the expression of the DC surface markers CD 80 , CD 83 and CD 86 indicating a maturation promoting effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After 5 days of culture , cell surface expression of MHC class I / II , CD 40 , CD 80 , and CD 86 was analyzed by flow cytometry , and nuclear NF kappaB proteins by electrophoretic mobility shift , antibody supershift , and Western blot assays . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DCs derived from GM CSF [ granulocyte / macrophage colony stimulating factor ] + interleukin ( IL ) 4 cultures expressed high levels of major histocompatibility complex ( MHC ) class 2 , CD 40 , CD 80 , and CD 86 molecules and were efficient stimulators of naive allogeneic T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interaction of CTLA 4 ( CD 152 ) with CD 80 or CD 86 inhibits human T cell activation . ^^^ It is concluded that interaction of CTLA 4 with its functional ligands , CD 80 or CD 86 , can down regulate human T cell responses , probably by intracellular signalling events and independent of CD 28 occupancy . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This effect is due to inhibition of the CD 40 pathway which normally leads to CD 80 and CD 86 up regulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Development of human T cell leukemia virus type 1 transformed tumors in rats following suppression of T cell immunity by CD 80 and CD 86 blockade . ^^^ Four week old WKA / HKm rats were treated with monoclonal antibodies ( MAbs ) to CD 80 and CD 86 and subcutaneously inoculated with syngeneic HTLV 1 infected TARS 1 cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The stimulatory hybrids expressed human leucocyte antigen ( HLA ) class 1 and 2 , and a wide range of surface accessory molecules , including the T cell co stimulatory ligand molecules CD 40 , CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , the expression of which was required for optimal stimulation of T cell responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Adhesion molecule ( CD 54 and CD 106 ) expression was not diminished by IL 10 treatment , and costimulatory molecule ( CD 80 and CD 86 ) expression was augmented by administration of exogenous IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of the costimulator molecules , CD 80 , CD 86 , CD 28 , and CD 152 on lymphocytes from neonates and young children . ^^^ The expression of CD 80 , CD 86 , CD 28 , and CD 152 were examined on peripheral blood lymphocytes from adults , neonates ( cord blood lymphocytes ) and young children ( 2 20 months of age ) . ^^^ There was no difference in the expression of CD 80 or CD 86 between adult and neonatal B cells , either resting or activated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blockade of the interaction of CD 28 with its ligands CD 80 and CD 86 using CTLA 4 Ig has been proposed as a therapy for a number of immune based disorders . ^^^ In vivo treatment with CTLA 4 Ig to block the interaction of CD 28 with CD 80 and CD 86 reduced virus specific cytotoxicity and IFN gamma production by bronchoalveolar lavage fluid CD8+ T lymphocytes in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory molecules CD 80 and CD 86 and their receptors CD 28 , CTLA 4 on malignant ascites CD3+ tumour infiltrating lymphocytes ( TIL ) from patients with ovarian and other types of peritoneal carcinomatosis . ^^^ Costimulation of T lymphocytes by the leucocyte surface molecules CD 80 and CD 86 expressed on antigen presenting cells ( APC ) is required for the development of T cell responses . ^^^ The CD 28 and CTLA 4 molecules on T cells serve as receptors for the CD 80 and CD 86 costimulatory antigens . ^^^ We have examined the frequency of expression of CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , CD 28 and CTLA 4 surface antigens on TIL isolated from malignant ascites or peritoneal washings of 26 patients with ovarian carcinoma and five patients with non ovarian peritoneal carcinomatosis . ^^^ Expression of CD 80 and CD 86 antigen was detected by reverse transcription polymerase chain reaction ( RT PCR ) , and by FACS analysis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After a 6 h incubation , phenotypic analysis of control , imiquimod , or R 848 treated LC indicated that such antigen presenting cells were in an `` intermediate ' ' state of maturation ( CD1a ( + ) , HLA DR , DP , DQ ( bright+ ) , CD 40 ( low+ ) , CD 86 ( high+ ) , and CD 80 ( low+ ) ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that an HLA DR+ subclone ( HEL DR+ ) expresses LFA 1 , LFA 3 , ICAM 1 , ICAM 3 , but neither CD 80 nor CD 86 on the surface . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
At day 7 post BLM , 48 % of CD11b+ BAMC displayed features of DC differentiation , as judged by dendritic morphology , expression of class 2 MHC , 33D1 , Factor XIIIa , CD 80 , and CD 86 antigens , and the ability to support a primary allogeneic lymphocyte response ( MLR ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis of surface antigen expression identified that after 5 days of culture the proliferating subpopulation of monocytes expressed CD 14 , CD 13 , CD 33 , CD11b , CD11c , CD 87 , HLA DR , CD45RO , and did not express CD 86 , CD 34 , CD 80 , CD 4 , CD 16 , and CD 56 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypically , CD11c + DC cultured fetal calf serum free homogenously coexpressed the DC specific molecule DEC 205 as well as the costimulatory molecules CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both cultures expressed class 2 MHC ( HLA DR ) , but the proportion of cells expressing the costimulatory molecules CD 80 and CD 86 was significantly less in cultures that developed from HNSCC patient CD34+ cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
New forms of dog CD 80 and CD 86 transcripts that encode secreted B 7 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The present review focuses on the role of two receptor ligand pairs : CD 28 and cytotoxic T lymphocyte associated antigen 4 , which interact with the B 7 ligands ( CD 80 , CD 86 ) ; and CD 40 , which interacts with the CD40L ( CD 154 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We studied the effect of toremifene on the expression of HLA DR , ICAM 1 , costimulatory molecules CD 80 and CD 86 , and the tumour necrosis factor receptor ( TNF R ) family molecules CD 27 , CD 30 , CD 40 , TNF R 1 , TNF R 2 and Fas ( CD 95 ) on MCF 7 breast cancer and Jurkat T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
One signal is delivered through the TCR by engagement with peptide / MHC complexes , and the second is delivered by interaction between costimulatory molecules such as CD 28 and its ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We quantitatively measured the number of T cells expressing cell surface activation associated molecules ( CD 69 , CD 25 , CD 122 , HLA DR ) and co stimulatory molecules ( CD 28 , CTLA 4 , CD 80 , CD 86 ) , including a Type 2 T cell marker ( CD 30 ) and CD11b , by flow cytometry of skin and peripheral blood . ^^^ CD 86 , CD 80 , CTLA 4 , CD 30 and CD11b were expressed by less than 23 % of the T cell populations from both the epidermis and dermis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The surface molecules CD 40 , CD 58 , CD 80 , CD 86 , CD 54 , and MHC class 1 molecules were up regulated in B CLL cells , whereas CD 95 expression was not influenced by CpG ODN stimulation . ^^^ Costimulation with CpG ODN and CD40LF resulted in further up regulation of CD 58 , CD 80 , CD 86 , and MHC class 1 molecules . ^^^ This effect was completely inhibited by a combination of anti CD 80 and anti CD 86 monoclonal antibody . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Induction of B 7 1 ( CD 80 ) and increased or sustained expression of CD44H , ICAM 1 ( CD 54 ) , and B 7 2 ( CD 86 ) are dependent on the interaction of CD 40 ligand with CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Vaccination with mouse mammary adenocarcinoma cells coexpressing B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) discloses the dominant effect of B 7 1 in the induction of antitumor immunity . ^^^ Nonreplicating TS / A mammary adenocarcinoma cells expressing B 7 2 ( CD 86 ) ( TS / A 2 ) are more immunogenic than those expressing B 7 1 ( CD 80 ) ( TS / A 1 ) , indicating that B 7 1 and B 7 2 display nonredundant costimulatory effects in inducing antitumor responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The Th 2 skewing of the developing immune response is explained by the effects of PDE inhibitors on several factors contributing to T cell priming : the cytokine milieu ; the type of costimulatory signal , i . e . , up regulation of CD 86 and down regulation of CD 80 ; and the Ag avidity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cyclosporine A was also the most effective drug downregulating the expression of accessory molecules ( CD 54 , CD 58 , CD 80 and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It is well known that tumor cells that express B 7 molecules can elicit antitumor immunity , but little is known regarding which B 7 molecule , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) , can do so more efficiently . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Professional APC are characterized by their ability to present peptide via HLA class 2 in the presence of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Addition of 1 , 25 ( OH ) 2D3 during LPS induced maturation maintains the immature DC phenotype characterized by high mannose receptor and low CD 83 expression and markedly inhibits up regulation of the costimulatory molecules CD 40 , CD 80 , and CD 86 and of class 2 MHC molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It was found that NOD macrophages , dendritic cells , and T cells , but not B cells , expressed lower basal levels of CD 86 , but not CD 80 , CD 28 , or CD 40 , compared with C57BL / 6 and BALB / c . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cells grew and differentiated into DCs as measured by a time dependent upregulation of cell surface antigens major histocompatibility complex class 2 , CD1a , CD 80 , CD 86 , CD 40 , and CD 4 , so that 40 % + / 9 % ( n = 6 ) of cells in culture at day 15 were CD1a+CD14 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The DC population thus obtained had a typical dendritic morphology and expressed DC surface markers , such as HLA class 2 , CD1a , CD11c , CD 54 , CD 80 and CD 86 , but not CD 14 for a period of up to three weeks in culture . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Further analysis of the FITClo cells from the DMBA treated mice demonstrated that these cells had reduced levels of CD 80 expression , had substantially reduced levels of CD 86 expression and performed poorly as co stimulator cells in an anti CD 3 mediated proliferative assay . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The co stimulatory signal provided by the interaction between CD 28 and its ligands , CD 80 and CD 86 , is critical for T cell activation . ^^^ In the present study , we examined the influence of anti allergic drugs on co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expression in normal mouse splenocytes by flow cytometry . ^^^ Treatment of BALB / c mice with azelastine ( AZ ) at a dose of 50 . 0 micrograms / kg / day , which is the most effective therapeutic dose , for two weeks scarcely affected the expression of co stimulatory molecules CD 40 , CD 80 and CD 86 on splenocytes , whereas a three week treatment strongly suppressed the expression of these molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , the CD40L dependent maturation of DCs is inhibited by MV infection , as demonstrated by CD 25 , CD 69 , CD 71 , CD 40 , CD 80 , CD 86 , and CD 83 expression down regulation . ^^^ In such DC PBL cocultures , inhibition of CD 80 and CD 86 expression on DCs was shown to require both MV replication and CD 40 triggering . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are glycoproteins expressed on antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Id primed tumor APC were heterogeneous , the majority being dendritic cells with class 2 ( + ) , CD11b ( + ) CD11c ( + ) CD 40 ( + ) CD 80 ( + ) CD 86 ( + ) markers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
All three spleen DC types would be considered as mature , based on expression of CD 80 , CD 86 , and CD 40 as well as on T cell stimulating function . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present study , expression of ( 1 ) CD 1 , a major histocompatibility complex class l like lipid antigen presenting molecule , ( 2 ) IL 10 , a cytokine promoting induction of clonal anergy , and ( 3 ) CD 80 and CD 86 , two T cell costimulators preventing induction of clonal anergy when present , was investigated in frozen sections of cervix , corpus and the fallopian tube ( FT ) of 25 day old BALB / c mice injected with FSH , progesterone or medium and of pregnant mice from each trimester ( days 7 , 14 and 19 ) . ^^^ CD 80 and CD 86 were usually absent from the female reproductive tract , but were occasionally found in the cervix during pregnancy . ^^^ This is supported by the absence of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
T cell costimulation is provided by ligation of CD 28 with either B7 . 1 ( CD 80 ) or B7 . 2 ( CD 86 ) on antigen presenting cells , and can be inhibited by a soluble form of CTLA 4 ( CTLA 4 Ig ) that binds to both B7 . 1 and B7 . 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BACKGROUND : B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , which are costimulatory molecules in T cell activation , play important roles in the differentiation of TH 1 or TH 2 phenotypes . ^^^ After in vitro stimulation , the expression of CD 80 and CD 86 on CD19+ cells was analysed by two colour flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , we tested the expression of CD 54 , CD 58 , CD 80 , and CD 86 on AEC 2 and evaluated their accessory function ( AF ) in cell culture using a coculture of interleukin 2 ( IL 2 ) , releasing Jurkat cells and AEC 2 . ^^^ AEC 2 also expressed CD 80 and CD 86 ( 38 % [ 0 77 % ] and 40 % [ 4 68 % ] , respectively ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) have complementary roles in mediating allergic pulmonary inflammation and airway hyperresponsiveness . ^^^ We examined the roles of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in a model of allergic pulmonary inflammation and airway hyperresponsiveness ( AHR ) by using mice with germline deletions of the B 7 1 and / or B 7 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Genetically modified tumors expressing various cytokines , major histocompatibility complex ( MHC ) molecules , or costimulatory molecules such as B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) can induce tumor specific immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We determined B lymphocytes ( CD 40 ( + ) ) , T lymphocytes ( CD 3 ( + ) ) , T helper ( CD 4 ( + ) ) , and T suppressor ( CD 8 ( + ) ) cells and the expression of costimulatory signals B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) on B cells and their counter receptors CD 28 and CTLA 4 on T cells by fluorescence activated cell sorting analysis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Immunophenotyping showed a low expression of CD 80 and CD 40 and a variable CD 86 expression on most AML cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These data reveal that Salmonella co incubation with BMDC as well as BMMPhi results in upregulation of MHC 1 and MHC 2 as well as several co stimulatory molecules including CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using RT PCR amplifications with mRNA from mitogen stimulated feline peripheral blood mononuclear cells , cDNA of feline B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were cloned . ^^^ Hydrophilicity patterns of the feline CD 80 and CD 86 which were only 26 . 8 % identical at the amino acid sequence were very distinct from each other , but similar to the putative human CD 80 and CD 86 proteins , respectively . ^^^ The feline CD 80 gene encoded a protein of 292 amino acids and the CD 86 gene encoded a protein of 329 amino acids . ^^^ Although the most conserved domain among the CD 80 sequences was the Ig C like domain , the most conserved domain among the CD 86 sequences was the Ig 5 like domain . ^^^ Among the known sequences , the bovine CD 80 and the porcine CD 86 sequences available for comparisons were identified as most closely related to the feline CD 80 ( 63 . 3 % ) and CD 86 ( 67 . 5 % ) , respectively . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interleukin 12 neutralization alters lung inflammation and leukocyte expression of CD 80 , CD 86 , and major histocompatibility complex class 2 in mice infected with Histoplasma capsulatum . ^^^ The present study was designed to determine if blockade of IL 12 disrupts the protective immune response by altering the influx of lineage specific cells into infected lungs and the numbers of cells expressing CD 80 , CD 86 , CD 119 , and major histocompatibility complex class 2 ( MHC 2 ) molecules . ^^^ CD 80 ( + ) lung cells from anti IL 12 treated mice were 2 to 3 fold greater than those from controls on days 7 and 10 , whereas the total numbers of CD 86 ( + ) cells were 2 to 3 fold less and MHC 2 ( + ) cells were 1 . 5 to 2 fold less on days 3 and 5 . ^^^ Treatment with monoclonal antibodies ( MAb ) to CD 80 , CD 86 , or both reduced the fungal burden slightly compared to that in rat immunoglobulin G treated controls , whereas after IL 12 neutralization , blocking of CD 80 reduced the tissue burden by 2 . 5 fold and this correlated with a decrease in IL 4 . ^^^ Regardless , mortality was not altered by treatment with MAb to CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules was much lower on LNC than on SC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The effect of B 7 mediated costimulation on T cell homeostasis was examined in studies of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) transgenic as well as B 7 deficient mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upon in vitro culture M DC8+ cells spontaneously mature into cells with the phenotype of highly stimulatory cells as documented by the upregulation of HLA DR , CD 86 and CD 40 ; in parallel CD 80 expression is induced . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IFN gamma favours this MPhi differentiation pathway via upregulation of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present study we determined the AF and the expression of CD11a , CD 54 , CD 58 , CD 80 , CD 86 and HLA DR costimulatory molecules by AMs lavaged from patients with pulmonary tuberculosis and controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cell surface phenotype of GM CSF DCs and IL 7 DCs was characterized by a high expression of major histocompatibility complex ( MHC ) class 1 and 2 , CD 80 , CD 86 and CD 40 . ^^^ Lipopolysaccharide ( LPS ) treatment induced the upregulation of CD 40 , CD 80 , CD 86 and the T cell stimulatory capacity in IL 4 DCs and , to a lesser extent , in the IL 7 DCs whereas GM CSF DCs responded very poorly to such treatment . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells express MHC Class 2 , CD11b , CD 14 , CD 32 , CD 68 and IL 10 , but not CD 40 , CD 80 or CD 86 , suggesting an immunosuppressive role for these dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In mammals , the classical B 7 molecules expressed on antigen presenting cells , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , bind the structurally related glycoproteins CD 28 and CTLA 4 ( CD 152 ) , generating costimulatory signals that regulate the activation state of T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Airway eosinophils , recovered after inhalational antigen challenge in sensitized mice , expressed MHC class 2 and costimulatory CD 80 and CD 86 proteins and functioned in vitro as CD 80 and CD 86 dependent , antigen specific , antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We conclude that the CD 40 ligand expressed by activated T cells first enhances MV replication in DCs , and then Fas ligand produced by activated T cells induces Fas mediated apoptosis of DCs , thus facilitating MV release . ( 2 ) Not only MV infected DCs but also bystander uninfected DCs undergo a maturation process confirmed by CD1a , CD 40 , CD 80 , CD 86 , CD 83 , and major histocompatibility complex type 2 labeling . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendritic cells were also stained for the costimulatory molecules CD 80 and CD 86 . ^^^ Fifty percent of DC expressed CD 86 and 20 % expressed CD 80 . ^^^ There was no difference in CD 80 or CD 86 expression between OB / BOS patients and stable patients . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Double staining studies using confocal laser scanning microscopy showed that the CD11c positive DCs coexpress both major histocompatibility ( MHC ) class 2 and costimulatory molecules , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD11c+ cells responded to LPS stimulation by producing high levels of interleukin 1beta ( IL 1beta ) and tumor necrosis factor alpha ( TNF alpha ) , and lower levels of IL 6 , IL 1Ra , and IL 8 and an increased expression of accessory molecules ( CD 25 , CD 40 , CD 80 , CD 86 , HLA DR , and HLA DQ ) . ^^^ PMA+I activation of CD11c+ cells resulted in high levels of IL 1beta and lower levels of IL 8 , IL 1Ra , and TNF alpha and up regulation of CD 80 , CD 86 , HLA DR , and HLA DQ . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of the costimulatory molecules CD 80 and CD 86 is differentially regulated by anti estrogens during DC differentiation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules such as CD 40 and CD 86 were slightly decreased or nonsignificantly affected ( CD 80 and MHC 2 ) . ^^^ However , after induction of DC maturation with LPS or incubation with CD 40 ligand transfected cells , D 3 DC showed marginal increases in MHC 1 , MHC 2 , CD 80 , CD 86 , CD 40 , and CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These brain DC were mature , as indicated by high level expression of MHC class 2 , CD 40 , CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have previously shown that B 7 2 ( CD 86 ) and , to a lesser extent , B 7 1 ( CD 80 ) contribute to the curative effectiveness of low dose melphalan ( L phenylalanine mustard ) for mice bearing a large MOPC 315 tumor under conditions that lead to the acquisition of potent cytotoxic T lymphocyte ( CTL ) activity at the tumor site . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This B cell effect required the presence of functional co stimulatory molecules CD 40 and B 7 2 ( CD 86 ) but not B 7 1 ( CD 80 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of major histocompatibility complex class 2 , CD 80 , CD 86 and CD11c by BMDC after phagocytosing rBCG or inert beads , was inhibited when the BMDC were pretreated with IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of molecules involved in antigen presentation and T cell activation ( HLA DR , CD 80 , CD 86 , CD 44 and CD 54 ) by cultured human osteoblasts . ^^^ BACKGROUND : Osteoblasts express the CD 44 antigen and HLA class 2 antigens , molecules which , together with other costimulatory molecules such as CD 80 , CD 86 , and CD 54 , are involved in antigen presentation and T cell activation . ^^^ Flow cytometry was used to examine the expression HLA DR , CD 80 , CD 86 , CD 44 , and CD 54 molecules involved in immune activities . ^^^ Although the cells were negative for CD 45 , the leukocyte common antigen and CD 14 ( an antigen detected on macrophages ) , they expressed CD 54 , CD 80 , and CD 86 antigens , which are also involved in the mechanisms of antigen presentation to and activation of T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Liver sinusoidal endothelial cells constitutively express all molecules necessary for antigen presentation ( CD 54 , CD 80 , CD 86 , MHC class 1 and class 2 and CD 40 ) and can function as antigen presenting cells for CD4+ and CD8+ T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cells with large , veiled cytoplasm , expressing major histocompatibility complex ( MHC ) class 2 and the costimulatory molecules CD 80 , CD 86 and CD 40 and lacking the monocyte marker CD 14 , were considered as having the phenotype of cytokine generated DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory effects of IL 2 and CpG ODN were investigated using proliferation assays , ELISA ( IL 6 , TNF alpha ) , and FACS analysis ( CD 80 , CD 86 expression ) . ^^^ In contrast , TNF alpha and IL 6 production was increased in B CLL cells , and the expression of CD 80 and CD 86 was further enhanced when IL 2 was used as a costimulus . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 engagement by specific monoclonal antibody ( mAb ) or binding of the natural ligands , CD 80 and CD 86 , induces tyrosine phosphorylation of CD 28 , which in turn recruits and activates the signal transducer and activator of transcription 6 ( Stat 6 ) . ^^^ The Stat 6 association with CD 28 is specifically induced by CD 80 or CD 86 ligand binding and is not dependent upon the secretion of IL 4 or IL 13 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After various intervals recovery of viable cells was measured and expression of CD 80 , CD 86 , CD 40 , CD 54 , CD 58 , and CD11a was analyzed by flow cytometry . ^^^ In cases with minor upregulation of CD 80 or CD 86 , no further stimulation using CD 40 L activation was observed . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Since the generation of a T cell response requires the expression of the costimulatory molecules CD 80 and CD 86 , we investigated their in vivo expression and their modulation in vitro during contact with responding T lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Proliferation , cytokine formation ( IL 6 , IL 8 , IL 1ra ) , and expression of surface molecules ( MHC class 1 and 2 , costimulatory molecules CD 80 and CD 86 , CD 29 , CD 54 , CD 95 ) were measured in stimulated and unstimulated cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Melanoma cells were shown to be able to co stimulate TCR triggered human T cells ; moreover , because they do not express CD 80 or CD 86 co stimulatory structures , the involvement of additional pathways have to be postulated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
For example , coadministration of costimulatory molecules ( CD 80 and CD 86 ) , proinflammatory cytokines ( interleukin 1alpha [ IL 1alpha ] , tumor necrosis factor alpha [ TNF alpha , and TNF beta ) , Th 1 cytokines ( interleukin 2 [ IL 2 ] , IL 12 , IL 15 , and IL 18 ) , Th 2 cytokines ( IL 4 , IL 5 , and IL 10 ) , and granulocytes macrophage colony stimulating factor ( GM CSF ) with DNA vaccine constructs leads to modulation of the magnitude and direction ( humoral or cellular ) of the immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
No differences in the surface expression of the costimulatory molecules CD 28 , CD 152 or CD 154 was seen between immune and non immune donors after either 24 or 120 h of TSL incubation , nor were differences detected in the expression of the B 7 ligands CD 80 or CD 86 on CD14+ monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Membrane expression of costimulatory ligands CD 80 and CD 86 was similar for native peptide and p 2 , 28 40A34 , 36 and increased in activation , whereas only CD 86 was elevated during anergy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , we show that the switch to either Th 1 or Th 2 responses is not effected by differential regulation through CD 80 or CD 86 and that a Th 2 response is achieved in the presence of IL 12 . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Up regulation of monocyte and B cell co stimulatory ligands CD4O , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were also inhibited in a dose dependent manner by pdFVIII , but LFA 3 ( CD 58 ) was unchanged . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC from both pGM CSF and FL treated mice expressed high levels of surface class 2 , low levels of the costimulatory molecules CD 40 , CD 80 , and CD 86 and were equally efficient at stimulating allogeneic and Ag specific T cell proliferation in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 was shown to be functionally intact by binding to its natural ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) both in vitro and in situ . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The influence of roxithromycin ( RXM ) on the expression of co stimulatory molecules , CD 40 , CD 80 and CD 86 , was examined in vivo . ^^^ RXM treatment of the immunized mice caused suppression of CD 40 expression , but this treatment did not affect CD 80 and CD 86 expression . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypically , there was no difference between migrated LCs from RAG 1 / or SCID mice vs normal C57BL / 6 or BALB / c mice : both populations of FITC+ cells had a dendritic morphology and a mature phenotype as they expressed high levels of MHC class 2 molecules and costimulatory molecules CD 80 , CD 86 , and CD 54 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD28 / CTLA 4 interactions with their specific B 7 ligands ( CD 80 and CD 86 ) have decisive roles in antigenic and allogenic responses . ^^^ CTLA 4 was observed in the infiltrating lymphocytes in most of the biopsies , but CD 80 or CD 86 were not . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To characterize the involvement of costimulatory pathways in the pathogenesis of myasthenia gravis ( MG ) , a multiparameter flow cytometry assay was adopted to enumerate blood mononuclear cells ( MNC ) expressing CD 28 , CD 80 , CD 86 , CD 40 , and CD40L molecules in patients with MG and healthy subjects . ^^^ Patients with MG had lower percentages of CD 8 ( + ) CD 28 ( + ) cells , augmented percentages of CD 4 ( + ) CD 80 ( + ) , CD 4 ( + ) CD 86 ( + ) , CD 8 ( + ) CD 80 ( + ) , CD 8 ( + ) CD 86 ( + ) , CD 14 ( + ) CD 80 ( + ) , and CD 14 ( + ) CD 86 ( + ) cells , and similar levels of cells expressing CD 40 and CD40L and of B cells expressing CD 80 and CD 86 compared to the controls . ^^^ The high percentages of CD 80 and CD 86 positive T cells and monocytes may reflect persistent activation of T and B cells , whereas the low CD 28 expression may be the result of chronic exposure to CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we show that administration of low dose melphalan ( l PAM , l phenylalanine mustard ) to mice bearing a large MOPC 315 plasmacytoma led to a rapid up regulation of B 7 1 ( CD 80 ) , but not B 7 2 ( CD 86 ) , expression on the surface of MOPC 315 tumor cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Incubation of immature murine dendritic cells with heparan sulfate induced phenotypic maturation evidenced by up regulation of 1 A , CD 40 , CD 54 ( ICAM 1 ) , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Both in vitro propagated normal liver derived DC , and freshly isolated bulk FL liver DC showed an immature phenotype ( MHC class 2 ( lo ) , CD 40 , CD 80 , and CD 86 ) and were weak stimulators of naive allogeneic T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Inhibiting p38SAPK prevented activation of the transcription factors ATF 2 and CREB and significantly reduced the LPS induced up regulation of CD 80 , CD 83 , and CD 86 , but did not have any significant effect on the LPS induced changes in macropinocytosis or HLA DR , CD 40 , and CD1a expression . ^^^ Inhibiting the NF kappaB pathway significantly reduced the LPS induced up regulation of HLA DR as well as CD 80 , CD 83 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On the other hand , TrkA stimulation does not change the expression of MHC , CD 80 , CD 86 , CD 40 , and CD 54 molecules , nor the antigen presenting function of monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cells displayed a characteristic iDC phenotype ( CD1a ( + ) , CD 14 ( ) , CD 80 ( + ) , CD 86 ( + ) , HLA DR ( + ) , CD 83 ( ) ) , and had potent allogeneic and antigen dependent autologous T cell stimulatory capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both CD 80 and CD 86 , expressed on transfected P 815 cells , were able to provide efficient costimulation for the production of IL 4 , IL 5 and IL 13 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This down regulation of the immune response was also observed when eukaryotic expression cassettes for CD 80 and for CD 86 were co administered with the pgD plasmid via the i . d . route . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that CD4+CD25+ T cells down regulated the expression of the co stimulatory molecules CD 80 and CD 86 on dendritic cells . ^^^ The steady state level of CD 80 mRNA was also decreased , while the steady state level of CD 86 mRNA was not , suggesting that distinct mechanisms regulate the expression of these molecules . ^^^ The down regulation occurred even in the presence of stimuli that would normally increase the expression of CD 80 and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This study examined the effects of blockade of the interaction between cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on the anticryptococcal DTH responses and protection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After coculture with anergic T cells expression of MHC class 2 , CD 80 and CD 86 by DC were down regulated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC infected with viable parasites up regulated the expression of CD 40 , CD 80 , CD 86 , and HLA DR and down regulated expression of CD 115 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of CD 68 , CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , HLA DR and CD 36 may also suggest that ELCs might be related to macrophages , sinus lining or microvascular endothelial cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Maturation of DC generated by this method can be induced by treatment with double stranded RNA , LPS or TNF alpha , resulting in enhanced surface expression of CD 80 , CD 86 , CD 40 , CD 83 and MHC molecules on the DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of costimulatory molecules ( CD 40 , CD 86 , CD 80 ) at the DC cell surface correlates with their capacity to induce or suppress immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show that the CD14+CD16++ cells express dendritic cell markers : CD 40 , CD 80 , CD 86 , HLA DR , CD11b , CD11c , CD 18 , CD1a , and CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The potential role of MRL / lpr B cells as APC , the expression of MHC class 2 molecules at their surface and their activation state ( expression of CD 69 , CD 80 / B 7 1 and CD 86 / B 7 2 molecules ) were studied . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They provide costimulatory signals ( in particular , CD 40 and the CD 28 ligands CD 80 and CD 86 ) necessary for naive T cell activation . ^^^ Functional expression of CD 80 and CD 86 is blocked by the fusion protein cytotoxic T lymphocyte antigen 4 immunoglobulin ( CTLA4Ig ) , that promotes tolerance induction in animals . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Anti human CD 80 and CD 86 antibodies alone had minor effects in the XMLR , but in combination with hu5C8 were as effective as human CTLA 4 Ig plus hu5C8 . ^^^ Anti hCD 80 and hCD 86 antibodies that did not cross react with porcine CD 80 or CD 86 were as effective in blocking the MLR as those that did cross react , indicating that the predominant costimulation in vitro was derived from the responding cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We also investigated by FACS analysis the cell surface expression of OX 2 , CD 80 , and CD 86 on DC incubated with ODN 1 blocking MD 1 expression . ^^^ Blocking MD 1 gene expression inhibits surface expression of CD 80 and CD 86 , but not of OX 2 . ^^^ By FACS , incubation of DC with anti MD 1 Ab to promote capping eliminated cell surface expression of MD 1 and CD 14 without altering DEC 205 , DC 26 , CD 80 , CD 86 , or OX 2 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In cytofluorometric analysis the morphological changes of cells were accompanied by upregulation of the expression of the MHC class 2 , CD11c , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Oncoselective transduction of CD 80 and CD 86 in tumor cell lines using an autonomous recombinant parvovirus . ^^^ Based on their oncotropic properties , we used autonomous recombinant parvoviruses to transduce the genes coding for the constimulatory molecules CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) specifically into tumor cells without transducing normal cells . ^^^ MATERIALS AND METHODS : After infection of tumor cells by these viruses , surface expression of CD 80 and CD 86 molecules was assessed by FACS and enhancement of immunostimulatory properties was assessed in alloreactions with G 10 purified T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although most of the HLA DM+ cells expressed CD1a , CD 80 or CD 86 antigen , only a small proportion of CD1a+ , CD80+ or CD86+ cells expressed HLA DM . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Concurrent reductions in B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , CD 40 , MHC class 2 , CD 83 , DC lysosomal associated membrane glycoprotein ( DC LAMP ) , and CD11c expression were detected on lesional DCs , which also decreased in number within lesional biopsies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Proliferation was substantially enhanced by anti CD 80 or anti CD 152 , but was inhibited by anti CD 86 . ^^^ Furthermore , anti CD 80 partially , and anti CD 152 totally protected cloned DO . 11 . 10 T cells from the induction of unresponsiveness following culture with peptide and Chinese hamster ovary H 2 Ad+ CD 80 CD 86 cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CTLA 4 receptor ( CD 152 ) on activated T lymphocytes binds B 7 molecules ( CD 80 and CD 86 ) on APC and delivers a signal that inhibits T cell proliferation . ^^^ However , pCTLA 4 Ig bound poorly to human CD 80 and CD 86 expressed on transfectants and EBV transformed human B cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Plasma cells from patients with myeloma express a tumor specific idiotype but lack CD 80 ( B 7 1 ) and have a variable expression of CD 86 ( B 7 2 ) . ^^^ The addition of huCD40LT to short term cultures up regulated both CD 80 and CD 86 expression on B cells ( CD 19 ( + ) ) and CD 80 on plasma cells ( CD 38 ( ++ ) ) , but did not up regulate CD 86 expression on plasma cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Accordingly , flow cytometric analyses revealed a dose dependent reduction of the expression of CD 40 , CD 80 , CD 86 , 1 A , and ICAM 1 on DC with a concurrent reduction of IL 12 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
EP and DSCG suppressed the expression of costimulatory molecules , CD 40 and CD 80 , but not CD 86 , on splenic B cells which were enhanced by antigenic stimulation in vitro . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cultured cells were analyzed by flow cytometry with respect to DC associated surface molecules ( CD1a , CD 83 , CD 40 , CD 80 , CD 86 , HLA DR ) when they showed significant DC morphology in culture ( 14 cases ) . ^^^ After cultivation , neo expression or upregulation of CD1a antigen was found in 8 samples , CD 83 in 2 , CD 40 in 14 , CD 80 in 7 , and CD 86 in 9 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vitro anti tumour activity of anti CD 80 and anti CD 86 immunotoxins containing type 1 ribosome inactivating proteins . ^^^ Immunotoxins specific for the CD 80 and CD 86 antigens were prepared by linking three type 1 ribosome inactivating proteins ( RIPs ) , namely bouganin , gelonin and saporin S 6 , to the monoclonal antibodies M 24 ( anti CD 80 ) and 1G10 ( anti CD 86 ) . ^^^ The anti CD 80 immunotoxins appeared 1 2 log more toxic for target cells than the anti CD 86 ones . ^^^ The expression of the CD 80 and CD 86 molecules is prevalently restricted to antigen presenting cells and is also strong on Hodgkin and Reed Sternberg cells in Hodgkin ' s disease . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this report , we show that , as a result of rAd infection , mouse bone marrow derived immature DC upregulate expression of major histocompatibility complex class 1 and 2 antigens , costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) , and the adhesion molecule CD 54 ( ICAM 1 ) . rAd transduced DC exhibited increased allostimulatory capacity and levels of interleukin 6 ( IL 6 ) , IL 12p40 , IL 15 , gamma interferon , and tumor necrosis factor alpha mRNAs , without effects on other immunoregulatory cytokine transcripts such as IL 10 or IL 12p35 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , the expression of the surface molecules CD 40 , CD 54 , CD 58 , CD 80 , CD 83 , CD 86 and the MHC class 2 molecules was upregulated after stimulation with PG . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Previous observations were extended to assess expression levels and regulatory mechanisms of costimulatory molecules CD 54 , CD 80 and CD 86 on monocytes derived from patients with early breast cancer ( EBC ) . ^^^ We report that monocytes derived from patients with EBC exhibited significantly decreased expression levels of CD 54 ( p = 0 . 0002 ) , CD 80 ( p = 0 . 009 ) and CD 86 ( p = 0 . 002 ) compared with monocytes derived from healthy females . ^^^ We conclude that monocytes derived from patients with EBC showed a simultaneous defect of expression of CD 54 and its regulation via TNF alpha , CD 80 and CD 86 as well as T cell proliferation following exposure to TT pulsed APC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Transduction with Ad IL 10 at a multiplicity of infection ( MOI ) of 50 100 resulted in a modest reduction in the incidence of DC expressing surface MHC class 2 , CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of the CD 80 and CD 86 molecules enhances cytotoxicity by human natural killer cells . ^^^ Both CD 80 and CD 86 function as costimulatory molecules in T cell cytotoxicity . ^^^ Several previous reports , most of them in the murine system , have indirectly or directly indicated the possible role of B 7 molecules ( CD 80 and CD 86 ) triggering NK cell mediated cytotoxicity in vitro . ^^^ Therefore , anti CD 80 and anti CD 86 mAbs were used in blocking experiments and both were shown to inhibit lysis by human NK cells . ^^^ The degree of inhibition observed was variable . 64 % of these NK clones were strongly inhibited by both anti CD 80 and anti CD 86 ( Type 1 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the presence of GM CSF and IL 4 , CB adherent cells became nonadherent , acquired DC morphology , and showed increased expression of CD1a , CD 80 , CD 86 , and HLA DR ; they lost membrane CD 14 and some cells with the expression of CD 83 and CMRF 44 were generated . ^^^ In the culture with TNF alpha , cells expressing CD1a , CD 80 , CD 86 , HLA DR , and HLA DQ were markedly increased . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The DC population obtained using ACBP showed higher levels of HLA class 2 molecules , co stimulatory molecules including CD 40 , CD 80 or CD 86 , and the dendritic cell marker CD 83 , compared with those generated in adult blood serum ( ABS ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mite antigens stimulated CD 80 and the major histocompatibility complex ( MHC ) class 2 molecule expression , but suppressed CD 86 expression on B cells and spleen adherent cells that were detected by a flow cytofluorometer . ^^^ Antibodies to the MHC class 2 molecules , CD 80 and CD 86 blocked the alloantigen presenting activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Genes under the control of virus late promoters were not expressed by 10 in DC , indicating an abortive infection . 10 infection selectively reduced the surface expression of the costimulatory molecule CD 80 and the DC maturation marker CD 83 on mature DC while other surface molecules including CD 86 and MHC remained unchanged . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Astrocytes and central nervous system endothelial cells do not express B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) immunoreactivity during experimental autoimmune encephalomyelitis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using flow cytometry , we showed that CD 80 and CD 86 are expressed at increased levels on human peripheral B cells in both groups after stimulation with anti CD 40 and interleukin 4 ( IL 4 ) , but to a significantly higher extent in the AD group . ^^^ Furthermore , baseline expression of CD 80 and CD 86 on peripheral B cells was low in normal donors and increased in AD donors . ^^^ These findings suggest that interaction of CD 80 and CD 86 with their receptors CD 28 and CTLA 4 selectively promotes cell activation , including proliferation and IgE production in CD40+IL 4 stimulated peripheral blood mononuclear cells from atopic donors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On the antigen presenting cell the key players are found in the extended family of B 7 genes comprising cd 80 , cd 86 , B7h / B7RP 1 and B 7 H1 . cd 80 and cd 86 encode proteins that bind to CD 28 and CTLA 4 on T cells . ^^^ While cd 80 and cd 86 pathways act mainly on naive T cells , B7h / B7RP 1 and B 7 H1 seem to exert their effects on antigen experienced lymphocytes . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Molecular cloning of the cDNAs encoding the feline B lymphocyte activation antigen B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) homologues which interact with human CTLA 4 Ig . ^^^ We cloned the cDNAs encoding the feline homologues of B lymphocyte activation antigens B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^ We expressed recombinant feline CD 80 and CD 86 molecules by the baculovirus expression system , and demonstrated their binding ability to human CTLA 4 murine immunoglobulin fusion protein . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The resulting cells exhibited the phenotype of mature DC , as assessed by the strong expression of HLA DR , CD 80 , CD 83 and CD 86 antigens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Like conventional monocyte derived DC , designated mDC 1 , mDC 2 expressed high levels of CD11c , CD 40 , CD 80 , CD 86 , and MHC class 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Endogenous PG production by MDC does not regulate CD 40 , CD 80 , CD 86 , or HLA DR expression ; however , it does promote MDC maturation , as NS 398 significantly reduces CD 83 expression in 1 MDC matured with sCD40L / IFN gamma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , pulsing of DC with serovar Typhimurium followed by incubation for 24 or 48 h altered surface expression of MHC 1 , MHC 2 , CD 40 , CD 54 , CD 80 , and CD 86 , generating a DC population with a uniform , high expression level of these molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 40 , CD 80 , and CD 86 expression by PG containing cells was higher in RA than in OA tissues . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We evaluated the levels of expression of costimulatory molecules ( CD 80 and CD 86 ) and the production of cytokines [ interleukin ( IL ) 12 , interferon ( IFN ) gamma , IL 4 , IL 10 ] in the cord blood of mononuclear cells in high risk newborns ( n = 17 ) , and compared them with those in low risk newborns ( n = 25 ) . ^^^ Fluorescence activated cell sorter ( FACScan ) analysis was performed to determine the expressions of CD 80 and CD 86 on activated B cells and monocytes of both groups . ^^^ The expressions of CD 80 and CD 86 on B cells showed no significant difference between the high and low risk group . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
No T or B cell invasion or involvement of co stimulatory B 7 molecules ( CD 80 and CD 86 ) was observed . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
ICOS binds B7h , a molecule expressed by APC with homology to CD 80 and CD 86 . ^^^ Strikingly , ICOS up regulation is significantly reduced in the absence of CD 80 and CD 86 and can be restored by CD 28 stimulation , suggesting that CD 28 CD80 / CD86 interactions may optimize ICOS expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Female Balb / c mice were given an intraperitoneal injection of 25 microg LPS and sacrified 24 h or 72 h later to determine total cell yield , lymphocyte subpopulations ( B cells , total T cells , CD4+ and CD8+ cells ) , the costimulatory molecules CD 28 , B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) and the percentage of apoptotic cells in PP and in the spleen as well as small intestinal IgA concentration . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The FL in vitro derived DCs , when treated with interferon alpha or lipopolysaccharide during the final 24 hours of culture , expressed an activated phenotype that included up regulation of MHC class 2 , CD1d , CD8alpha , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We now extend our observations in 71 DLCL patients by analyzing malignant B cell expression of the following molecules important in T cell activation : ( a ) recognition molecules [ MHC 1 ( MAS and MCA ) and MHC 2 ( HLA DR , DP , DQ ) ] ; ( b ) CAMs [ leukocyte function antigen 1 ( CD11a and CD 18 ) and intracellular adhesion molecule 1 ( CD 54 ) ] ; and ( c ) CSMs [ B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) ] . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A significant increase in monocyte expression of CD 80 , CD 86 , CD 40 , and human leukocyte antigen DR ( HLA DR ) was also induced by live influenza virus . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The 5 2 , Ra 2 , and 6 3 clones expressed CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and MHC class 2 at low , medium , and high levels , respectively . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , thymic peptides induced the up regulation of costimulatory molecules including CD 86 , CD 80 , HLA DR and HLA ABC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show that exposure of MODC to IS PL , but not IS ODN , induced a dose dependent strong up regulation of HLA class 2 and co stimulatory molecules ( CD 80 , CD 86 ) , similar to that observed after treatment with TNF alpha . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Two populations of DC were defined : immature DC were uniformly CD1a ; mature DC expressed CD1a , CD 80 , CD 86 , HLA DR , CD 54 and CD 58 but lacked surface CD 14 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Surface expression of CD 40 , CD 80 , CD 83 , and CD 86 was increased by BCG CWS on human immature DC , and the effect was similar to those of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , heat killed BCG , and viable BCG . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
HIV 1 infected LCs downregulated surface CD 4 and CD 83 , whereas MHC class 2 , CD 80 , and CD 86 were unchanged . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both types of neonatal EPC acquired class 2 in culture , and up regulated CD 80 and CD 86 in an adult pattern , but failed to up regulate class 2 antigen to the high level seen among cultured adult cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of CD 80 , CD 86 , human leukocyte antigen ( HLA ) DR , HLA ABC , and intracellular adhesion molecule 1 ( ICAM 1 ) was tested in human AML cell lines after the addition of HDACI , sodium butyrate ( SB ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In comparison to splenic DCs , thyroid DCs had a low CD 80 and CD 86 expression in both strains ( i . e . , an ' immature ' phenotype ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Glucocorticoids hamper the ex vivo maturation of lung dendritic cells from their low autofluorescent precursors in the human bronchoalveolar lavage : decreases in allostimulatory capacity and expression of CD 80 and CD 86 . ^^^ Here , we also show that CD 80 and CD 86 are normally expressed at low levels on CD1a+ LAF cell derived DCs compared to other DC populations . ^^^ This low level expression of costimulatory molecules is discussed here in relation to the previously reported low level expression of CD 80 ( and CD 86 ) on lung DCs in experimental animals . ^^^ DEX exposure of CD1a+ LAF cells prevented the upregulation of even this low level expression of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometry revealed an up regulation in the expression of CD 80 , CD 86 and CD 83 following activation with a soluble trimeric form of CD40L ( CD40Ls ) or LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
For this purpose , we have generated a whole series of isogenic virus stocks ( NL 4 3 backbone ) bearing or not bearing on their surface foreign CD 28 , CD 54 ( ICAM 1 ) , CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) proteins . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dex inhibited the expression of both CD 80 and CD 86 on THP 1 cells , a human acute monocytic leukemia cell line , as determined by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
There were no differences between adherent and floating DC regarding expression of CD11c , OX 62 , major histocompatibility complex class 2 , CD 80 , or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Laboratory findings included lymphocytopenia , highly elevated CD45RA / CD45R0 ratio , as well as reduced expression of the co stimulatory molecules CD 154 , CD 86 , CD 80 and CD 28 on CD4+ cells in combination with disturbed lymphocyte transformation in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In parallel , the expression by monocytes of co stimulatory molecules ( CD 40 , CD 80 , CD 86 ) , major histocompatibility complex molecules ( HLA ABC , HLA DR ) and Fcgamma receptors ( CD 16 , CD 64 ) was examined by flow cytometry . ^^^ MS patients ' blood monocytes also displayed elevated mean fluorescence intensity for the co stimulatory molecule CD 86 , and MS patients with longer disease duration ( > 10 years ) and higher disease severity ( EDSS > 3 ) had higher percentages of CD 80 expressing monocytes compared to patients with short duration or lower severity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Murine bone marrow cultured with GM CSF produced dendritic cells ( DCs ) expressing MHC class 2 ( MHC 2 ) but little CD 40 , CD 80 , or CD 86 . ^^^ Oligodeoxynucleotides ( ODN ) containing CpG motifs enhanced DC maturation , increased MHC 2 expression , and induced high levels of CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulation light : activation of CD4+ T cells with CD 80 or CD 86 rather than anti CD 28 leads to a Th 2 cytokine profile . ^^^ Another set of beads was prepared by immobilizing anti CD 3 and murine CD 80 Ig fusion protein or murine CD 86 Ig fusion protein on the beads . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
FDCP mix cells were cultured in various amounts of GM colony stimulating factor ( CSF ) and interleukin ( IL ) 4 for up to 16 d and analysed for morphology , expression of CD 34 , c kit , Gr 1 , Mac 1 , CD 40 , MHC 1 , MHC 2 and co stimulatory molecules ( CD 80 , CD 86 ) using flow cytometry , and for their capacity to present foreign antigen to autologous T cells . ^^^ The dendritic cells generated from FDCP mix cells were large , non adherent cells with veiled processes and expressed MHC 2 , CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Polymophonuclear cells ( PMN ) of healthy donors do not express major histocompatibility complex ( MHC ) class 2 antigens or the T cell costimulatory molecules CD 80 or CD 86 . ^^^ We now report that , by culturing PMN of healthy donors with autologous serum , interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , de novo synthesis of MHC class 2 , CD 80 and CD 86 could be induced . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition to CD 80 and CD 86 , the host derived 4 1BBL is also involved in the secondary anti tumour responses . ^^^ This study indicates the complicated contribution of 4 1BBL , CD 80 and CD 86 on tumour and host cells in anti tumour immune responses and a possible therapeutic application of 4 1BBL for human tumour vaccination and gene therapy . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 154 is an activation induced CD4+ T cell surface molecule that interacts with CD 40 on antigen presenting cells ( APC ) and upregulates the key costimulatory molecules , CD 80 and CD 86 . ^^^ This review will focus on the immunobiology of CD 154 CD40 interactions and the costimulatory functions of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Most cells expressed CD 80 but only a small percentage of cells were positive for CD 40 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Chronic stimulation ( 24 h ) of DCs with low , noncytotoxic ATP doses increased membrane expression of CD 54 , CD 80 , CD 86 , and CD 83 , slightly reduced the endocytic activity of DCs , and augmented their capacity to promote proliferation of allogeneic naive T lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulation with lipopeptides increased expression of CD 83 , MHC class 2 , CD 80 , CD 86 , CD 54 , and CD 58 , and decreased CD 32 expression and endocytic activity ; these lipopeptide matured DC also displayed enhanced T cell stimulatory capacity in MLR , as measured by T cell proliferation and IFN gamma secretion . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The accessory cell function of AML blasts showed no correlation with the release of any single immunomodulatory soluble mediator ( IL1beta , IL 6 , TNF alpha , soluble IL 2 receptors ) or the expression of any particular adhesion / costimulatory membrane molecule ( CD 54 , CD 58 , CD 80 , and CD 86 ) by the blasts . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mature DC from PBMC or CD 34 ( + ) cells had similar enhanced expression of MHC class 2 HLA DR , CD 80 , CD 83 , and CD 86 and were potent stimulators of mixed lymphocyte reactions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Extended studies demonstrated that : 1 ) ASM cells express mRNAs and proteins for the cell adhesion molecules ( CAMs ) / costimulatory molecules , CD 40 , CD40L , CD 80 , CD 86 , ICAM 1 ( CD 54 ) , and LFA 1 ( CD11a / CD18 ) ; 2 ) apart from LFA 1 , ASM cell surface expression of the latter molecules is up regulated in the presence of activated T cells ; and 3 ) pretreatment of ASM cells and tissues with mAbs directed either against CD11a or the combination of CD 40 and CD 86 completely abrogated both the activated T cell induced changes in expression of the above CAMs / costimulatory molecules in ASM cells and altered ASM tissue responsiveness . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression and function of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in an in vitro model of the human blood brain barrier . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immature DCs ( CD 80 and CD 86 deficient ) were derived from bone marrow cells propagated in GM CSF and TGF beta 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Other immunologically important molecules detected included the co stimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The importance of CD 40 , CD 80 , and CD 86 costimulatory molecules in anti Leishmania immune responses has been established in murine models . ^^^ Blockade of CD 80 , CD 86 , or both molecules ( in the order of greatest effect ) in cultures containing macrophages , PBL , and L . major significantly inhibited the production of gamma interferon , interleukin 5 ( IL 5 ) , and IL 12 . ^^^ Thus , these data suggest that CD 40 , CD 80 , and CD 86 expression and regulation may significantly impact anti Leishmania immune responses in humans . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
There are three identified members of this family : CD 80 , its splice variant IgV CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Paradoxically , while decreasing CD 40 and MHC class 1 expression , betaMv significantly increased the expression of MHC class 2 , CD 80 , and CD 86 on cultured LCs but impaired their allostimulatory activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This was reflected by an inhibition of CD1a , CD 40 , CD 80 , CD 86 , and HLA DR expression as well as by a reduced capacity of dimethylfumarate treated monocyte derived dendritic cells to stimulate lymphocytes in the allogeneic mixed lymphocyte reaction . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results demonstrate that quantitation of the capacity of CD 8 ( + ) CD 28 ( ) T cells from patient circulation to suppress the activation of costimulatory molecules ( CD 80 , CD 86 ) on donor APC offers a reliable tool for monitoring specific immunosuppression against the graft in solid organ transplantation . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , conditioned medium of discrete melanoma lines induced upregulation of CD 80 , CD 86 , MHC class 1 , and MHC class 2 molecules on immature DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We analyzed the phenotype of CB monocytes in the presence or absence of an inflammatory signal ( rIFN gamma ) and compared them to adult blood ( AB ) ; the expression of HLA DR and 17 different markers ( CD11a , CD11b , CD11c , CD 18 , CD 29 , CD 40 , CD 44 , CD49a , CD49d , CD49e , CD49f , CD 54 , CD 58 , CD62L , CD 80 , CD 86 and CD 102 ) was measured by flow cytometry . ^^^ Statistical analysis showed that , compared to AB , CB monocytes did not express CD11b , CD11c , CD49d and after stimulation with rIFNgamma , they lost the expression of CD 58 and CD 102 , whereas CD 80 and CD 86 expression was induced . ^^^ However , CB monocytes expressed CD 80 and CD 86 co stimulatory molecules , but this expression did not prove a normal co stimulatory function . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Thus , mast cells can phagocytize diverse particles , take up antigens , and express a number of receptors , particularly MHC class 1 and 2 antigens , ICAM 1 and 3 , CD 43 , CD 80 , CD 86 and CD40L which allow them to interact with T and B lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the M cell areas , CD 80 / CD 86 expression was much more frequent on memory ( sIgD ( ) CD 20 ( + ) ) B cells than on naive ( sIgD ( + ) CD 20 ( + ) ) B cells . ^^^ M cell areas identified by such co expression of CD 20 and CD 80 / CD 86 were always spatially related to germinal centers ( GC ) . ^^^ Contrary to the GC B cell phenotype ( sIgD ( ) CD 20 ( + ) CD 80 / 86 ( hi ) CD 10 ( + ) Bcl 2 ( ) ) , however , M cell associated B cells with a high level of CD 80 / CD 86 were CD 20 ( lo ) CD 10 ( ) Bcl 2 ( + ) , and adjacent memory T cells ( CD 3 ( + ) CD45R0 ( + ) ) often expressed CD40L ( CD 154 ) . ^^^ Autologous peripheral blood B T cell cocultures with purified protein derivative as antigen showed that the sIgD ( ) CD 80 / CD 86 ( hi ) CD 20 ( lo ) phenotype could indeed be generated during cognate B T interactions , concurrent with CD40L up regulation on memory T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The pattern of costimulatory molecule expression ( CD 80 , CD 86 ) was primarily examined using immunohistochemistry on tissue specimens of bone / implant interface membranes taken from sites of bone erosion . ^^^ In vitro analysis also indicated phagocytosis of metal particles by differentiated U 937 caused significant induction of both CD 80 and CD 86 ( p < 0 . 01 ) , although the expression of CD 86 dominated following prolonged exposure . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
New polymorphisms of human CD 80 and CD 86 : lack of association with rheumatoid arthritis and systemic lupus erythematosus . ^^^ The crucial role of costimulatory molecules , CD 28 , CTLA 4 , CD 80 and CD 86 , for T cell activation and inhibition has been established . ^^^ In this study , we screened for polymorphisms of human CD 28 , CD 80 and CD 86 genes , and detected that polymorphisms were tested for the association with rheumatoid arthritis ( RA ) and systemic lupus erythematosus ( SLE ) . ^^^ Variations were identified in the coding regions of CD 80 ( 452G / A , 614C / G and 864A / G ) and CD 86 ( 1057A / G ) , while no variation was observed in the coding region of CD 28 . ^^^ The variations at CD 80 position 452 and CD 86 position 1057 were present in a substantial proportion of the Japanese population , and were considered to be single nucleotide polymorphisms within the coding sequence ( cSNPs ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CpG oligodeoxynucleotide ( CpG ODN ) , but not control ( non CpG ODN ) , increased the expression of costimulatory molecules ( CD 40 , CD 80 , CD 86 , CD 54 ) on malignant B cells without altering the phenotype of B cells obtained from reactive follicular hyperplasia . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They expressed insufficient amounts of MHC class 2 , CD 80 and CD 86 molecules on the surface of the cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The myeloid dendritic cells in CSF expressed higher levels of HLA DR , CD 86 , CD 80 and CD 40 than those in blood , whereas expression of these molecules by plasmacytoid dendritic cells was equal in blood and CSF . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Contemporary , no clear evidences of a role for other surface molecules modulated during DC maturation ( CD 80 , CD 83 , CD 86 and CD 40 ) , were obtained . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , AGC 10 did reduce the levels of expression of CD 86 and CD 80 , molecules known to play critical roles in B cell interactions with T lymphocytes . ^^^ Fairly large subpopulations of , but not all , B lymphocytes had their expression of CD 122 ( + ) , CD 132 ( + ) , CD 86 ( + ) and CD 80 ( + ) reduced to undetectable levels in the presence of AGC 10 . ^^^ This was denoted by comparable mean fluorescence intensity values representing the expression of CD 122 , CD 132 , CD 86 or CD 80 molecules on the surface of SACI stimulated CD 19 ( + ) cells cultured without or with AGC 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Such leukemia derived DCs expressed a sufficient level of costimulatory molecules ( CD 80 and CD 86 ) , and were shown to be monoclonal based on an the 10 inactivation analysis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we used the same model to examine the requirement for CD 28 B7 mediated T cell costimulation in the progression of established chronic rejection and examined the individual roles of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulatory molecules . ^^^ Late blockade of CD 28 B7 T cell costimulation by the fusion protein CTLA4Ig , which binds both CD 80 and CD 86 , attenuated the development of transplant arteriosclerosis , mononuclear cell infiltration , and parenchymal fibrosis in this model . ^^^ This study further defines functional differences between CD 80 and CD 86 costimulatory molecules in vivo . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
SB 203580 , an inhibitor of the p 38 MAPK , but not the extracellular signal regulated kinase l / 2 pathway blocker PD 98059 , inhibited the up regulation of CD1a , CD 40 , CD 80 , CD 86 , HLA DR , and the DC maturation marker CD 83 induced by LPS and TNF alpha . ^^^ DC maturation induced by the contact sensitizers 2 , 4 dinitrofluorobenzene and NiSO ( 4 ) , as seen by the up regulation of CD 80 , CD 86 , and CD 83 , was also coupled to the phosphorylation of p 38 MAPK , and was inhibited by SB 203580 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Following enrichment , DC developed an activated phenotype with up regulation of CD 80 , CD 86 , and CD 83 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This study assessed the value of morphological grading and the expression of the co stimulatory markers CD 40 , CD 80 and CD 86 and their ligands to predict clinical outcome in 23 consecutive low grade MALT lymphoma patients treated with H . pylori eradication . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 12 gene modified DCs ( DC / IL 12 ) displayed a more mature phenotype than nontransduced DCs , as judged by decreased expression of CD1a and increased expression of CD 83 , B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , and MHC class 1 and 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IFN beta , together with GM CSF and IL 4 , also induced maturation of IL 3Ralpha expressing cells , as reflected by upregulation of HLA DR and of the costimulatory molecules CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this review , we summarize the current understanding of these complex costimulatory pathways including the individual roles of the CD 28 , CTLA 4 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Evaluation of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulatory molecules and dendritic cells on the immune response in leprosy . ^^^ In leprosy , the role of CD 80 and CD 86 as costimulatory signal in M . leprae specific cellular immunity has not yet been defined . ^^^ The cells were stimulated by M . leprae in the presence of anti CD 80 antibody ( Ab ) and / or anti CD 86 antibody ( Ab ) ( Innogenetics ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using a range of APC markers , including CD 68 , HAM 56 , HLA DR , CD1a , p 55 , RFD 1 , and costimulatory molecules CD 40 , CD 80 , and CD 86 we document the close phenotypic resemblance of rhesus and human APC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After such enhanced clustering the DCs had increased their T cell stimulating capabilities in syngeneic mixed lymphocyte reaction , and had a higher expression of CD 80 and CD 86 ( signs of maturation ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting analysis showed that instant dendritomas express the key molecules for antigen presentation ( HLA A , B , C ; HLA DR ; CD 80 ; and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotype and allostimulatory function of dendritic cells treated with antisense oligodeoxyribonucleotides targeting CD 80 or CD 86 mRNA . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Administration of antisense oligodeoxyribonucleotides against mRNA of CD 80 or CD 86 prolongs survival of cardiac allografts by inhibition of CTL activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On the 7th day of culture , the cells became mature DC with a CD1a ( + ) , CD11c ( + ) , CD 80 ( + ) , CD 83 ( + ) , CD 86 ( + ) , human leukocyte antigen ( HLA ) DR ( + ) , CD 14 phenotype . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IRF 1hER stably expressing murine Hepa 1 6 HCC cells ( HepaIRF 1hER ) were characterized by lowMHC 1 , highCD 54 , and lack of MHC 2 , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CD 28 ligands CD 80 and CD 86 are expressed on APC , and both provide costimulatory function . ^^^ We have previously shown that blockade of CD 80 costimulation by Y100F Ig , a CTL associated Ag 4 ( CTLA 4 ) Ig mutant that does not bind CD 86 , inhibits the development of lung inflammatory immune responses , but does not affect blood eosinophilia or Ab production . ^^^ Each of those responses was inhibited by treatment with CTLA 4 Ig , which binds both CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Addition of an anti IL 10 neutralizing Ab to immature DCs as well as to soluble CD 40 ligand or LPS maturing DCs led to enhanced expression of surface CD 83 , CD 80 , CD 86 , and MHC molecules and markedly augmented release of TNF alpha and IL 12 , but diminished IL 10 mRNA expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recipient mice were given donor 1x10 ( 7 ) splenocytes into the trachea with or without antibody specific for mouse CD 80 ( 1G10 ) and / or CD 86 ( GL 1 ) ( 100 microg each ) 7 days before transplantation . ^^^ CONCLUSION : Intratracheal delivery of alloantigen induced significantly prolonged survival of fully mismatched cardiac allografts and the effect was abrogated by the blockade CD 80 and / or CD 86 pathway . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : The expression of HLA DR , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) molecules was determined on CD14+ monocytes of chronic hemodialysis patients prior to a dialysis session . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These day 0 ( d 0 ) CD11c ( + ) HLA DR ( + ) lin ( ) cells comprised approximately 0 . 6 % of the mononuclear cells obtained from the lamina propria , were endocytically active , and had the phenotype of immature DC ; they were CD 40 ( + ) and expressed low levels of CD 83 and CD 86 , but little or no CD 80 or CD 25 . ^^^ The lamina propria also contained a population of cells capable of migrating out of biopsies during an overnight culture and differentiating into mature DC with lower levels of endocytic activity and high cell surface expression of CD 40 , CD 80 , CD 86 , CD 83 , and CD 25 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NOD DC , in contrast to CBA DC , have very low levels of intracellular 1 A molecules and cell surface expression of MHC class 2 , CD 80 , CD 86 and CD 40 but normal beta 2 microglobulin expression . ^^^ Incubation with the strong inflammatory stimulus of LPS and IFN gamma does not increase class 2 MHC , CD 80 or CD 86 , but upregulates the level of CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OBJECTIVES : To investigate the expression and regulation of CD 80 , CD 86 , and CD 28 costimulatory molecules in sialoadenitis and interstitial nephritis in patients with Sjgren ' s syndrome ( SS ) . ^^^ METHODS : Expression of CD 80 , CD 86 , and CD 28 molecules was studied by immunohistochemical staining of lip biopsy specimens obtained from patients who had sialoadenitis associated with SS , and renal biopsy specimens obtained from patients who had interstitial nephritis associated with SS . ^^^ To elucidate the mechanism of de novo expression of CD 80 and CD 86 antigens , their induction by cytokines in human salivary duct cell line ( HSG ) and renal cortical epithelial cells ( HRCE ) by cell enzyme linked immunosorbent assay ( ELISA ) was quantitatively investigated . ^^^ RESULTS : In patients with severe sialoadenitis , CD 80 and CD 86 were strongly expressed on ductal epithelial cells . ^^^ In patients who had interstitial nephritis associated with SS , some tubular epithelial cells expressed CD 86 but not the CD 80 antigen . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Isolated peripheral blood lymphocytes were stained for the lymphocyte subset markers CD 4 , CD 8 , CD 19 , their respective activation markers CD 25 , HLA DR , CD 38 , and the costimulatory molecules CD40L , CD 28 , CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) play important roles in the differentiation of Th 1 and Th 2 phenotypes . ^^^ CD 40 CD40L interaction supports the expression of CD 80 and CD 86 on antigen presenting cells . ^^^ We investigated the roles of CD 80 , CD 86 , and CD 40 in the differentiation of Th 1 and Th 2 subsets after stimulation with the antigen in subjects with cedar pollinosis . ^^^ After in vitro stimulation , CD 80 , CD 86 , and CD 40 expression on CD19+ cells were analyzed by flow cytometry . ^^^ These results indicate that the Th 2 response predominated in pollinosis subjects and that CD 86 rather than CD 80 may be the costimulatory molecule involved in the allergen induced activation of PBMC . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our data showed that Dex , in a dose dependent manner , down regulated surface expression of CD 86 , CD 40 , CD 54 and MHC class 2 molecules by DC , but the expression of MHC class 1 , CD 80 , CD 95 and CD95L were not affected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
VLP were found to bind very well to human and mouse immune cells that expressed markers of antigen presenting cells ( APC ) such as MHC class 2 , CD 80 and CD 86 , including dendritic cells , macrophages and B cells . mAb blocking studies identified Fc gamma RIII ( CD 16 ) as one of the molecules to which the VLP can bind both on immune cells and foreskin epithelium . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The mechanism for this is that NF kappaB is essential for three aspects of antigen presenting function : blocking NF kappaB coordinately down regulates HLA class 2 , co stimulatory molecules like CD 80 , CD 86 and CD 40 , and immuno stimulatory cytokines like IL 12 and tumor necrosis factor alpha . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The surface phenotype of purified tumor APCs MB was CD11b ( + ) , CD11c ( + ) , CD 40 ( + ) , CD 80 ( + ) , CD 86 ( + ) , and MHC class 2 ( + ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The responses to lactoglobulin and PHA were inhibited by the CTLA4 / Fc chimera and by MAbs against CD 2 , CD 58 , CD 80 , and CD 86 , indicating stimulation of CD28+ LPLs with antigen presenting cells through activation of the CD 2 pathway . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Following 24 h of exposure to the fungus , DC displayed an increase in the mean fluorescence intensity of HLA DR , CD 80 , and CD 86 , and an increase in the percentage of CD 54 ( + ) cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
An immunoperoxidase technique was used to examine CD 28 , CD 152 , CD 80 and CD 86 positive cells in gingival biopsies from 21 healthy / gingivitis and 26 periodontitis subjects . ^^^ Analysis of these cells in relation to increasing numbers of B cells in proportion to T cells and also to macrophages , suggested that CD 80 was expressed predominantly by macrophages while CD 86 was expressed by both macrophages and B cells . ^^^ Few endothelial cells expressed CD 80 or CD 86 . ^^^ Keratinocytes displayed cytoplasmic staining of CD 80 rather than CD 86 although the numbers of positive specimens in the healthy / gingivitis and periodontitis groups reduced with increasing inflammation . ^^^ In conclusion , percentages of CD 28 , CD 152 , CD 80 and CD 86 did not reflect differences in clinical status . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypically , the KG 1 derived DCs lack lineage specific markers , and express MHC class 2 , costimulatory molecules CD 80 and CD 86 , and CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The donor DCs were CD 80 ( + ) CD 86 ( + ) and , ultrastructurally , were in intimate contact with lymphoblasts within the rosettes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential role of CD 80 and CD 86 on alveolar macrophages in the presentation of allergen to T lymphocytes in asthma . ^^^ In the asthmatic lung the altered expression of costimulatory molecules ( CD 80 , CD 86 ) by alveolar macrophages contributes to T lymphocyte activation and expansion . ^^^ We hypothesized that CD 80 and CD 86 on alveolar macrophages could differentially support allergic inflammation in adult asthma . ^^^ The expression of CD 80 and CD 86 costimulatory molecules by alveolar macrophages was studied and , in parallel , the efficiency of antigen presentation was measured in terms of IL 4 and IL 5 production by allergen stimulated autologous T cells . ^^^ We found that in asthmatic subjects ( 1 ) the percent of CD80+ , but not CD86+ alveolar macrophages was increased at baseline and did not change following allergen challenge ; ( 2 ) CD 86 , but not CD 80 , membrane expression was up regulated following allergen challenge ; ( 3 ) both CD 80 and CD 86 were required to support Th 2 cytokine production by allergen specific T cells , with a prevalent role of CD 86 after allergen challenge . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , the surface expression of the costimulatory molecules CD 40 , CD 54 , CD 80 , and CD 86 was increased , indicating that CEACAM 1 induced signaling regulates early maturation and activation of dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
HOT increased the number of both CD4+ T cells and antigen presenting cells expressing MHC class 2 as well as costimulatory molecules ( CD 40 , CD 80 and / or CD 86 ) in the Peyer ' s patch ( PP ) of infant mice , which had fewer cells than adult mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Antibodies to the other co stimulatory molecules CD 40 ligand , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) , although inhibitory , were less effective . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
KM H 2 cells were demonstrated to carry the newly proposed DC associated molecules fascin , CD 83 , and DEC 205 , as well as costimulatory molecules such as CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Analysis of CD 80 and CD 86 expression showed that LC from day 3 skin expressed CD 80 , but not CD 86 and application of antigen through this skin was inefficient in upregulating CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We designed single chain Fv antibodies , monospecific and bispecific diabodies , and a bispecific tetravalent antibody ( BiTAb ) molecule directed against the CD 80 and / or CD 86 costimulatory molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
None of them expressed the immunophenotypic markers of the lymphocyte ( CD 20 , CD45RO , CD 4 , CD 8 , TCRgammadelta ) , mature monocyte ( CD 33 ) , or dendritic cell ( CD1a , CD 80 , CD 86 , S 100 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , at physiological concentrations , it profoundly inhibited CD 40 , CD 80 , CD 86 , and MHC class 2 expression on murine , GM CSF + IL 4 stimulated , bone marrow derived myeloid dendritic cells ( DC ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Infection of immature DC also led to selective and asynchronous downregulation of CD1a , CD 40 , CD 54 ( ICAM 1 ) ( 12 h postinfection ) , CD 80 ( 24 h postinfection ) , and CD 86 ( 48 h postinfection ) but not of CD11c or major histocompatibility complex class 1 and 2 molecules when compared to DC exposed to UV inactivated virus . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential incorporation of CD 45 , CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and major histocompatibility complex class 1 and 2 molecules into human immunodeficiency virus type 1 virions and microvesicles : implications for viral pathogenesis and immune regulation . ^^^ Because T cell receptor engagement of major histocompatibility complex ( MHC ) molecules in the absence of costimulation mediated via CD 28 binding to CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) can lead to anergy or apoptosis , we determined whether HIV type 1 ( HIV 1 ) virions incorporated MHC class 1 ( MHC 1 ) , MHC 2 , CD 80 , or CD 86 . ^^^ HIV infection increased MHC 2 expression on T and B cell lines , macrophages , and peripheral blood mononclear cells ( PBMC ) but did not significantly alter the expression of CD 80 or CD 86 . ^^^ HIV virions derived from all MHC 2 positive cell types incorporated high levels of MHC 2 , and both virions and microvesicles preferentially incorporated CD 86 compared to CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The surface expression of CD 14 , CD 54 , CD 64 , CD 80 , CD 86 and HLA DR was determined by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Exposure of immature DCs to CD40L transgenic cells , soluble recombinant human CD40L molecules or lipopolysaccharide induced expression of the co stimulatory molecules , CD 80 and CD 86 , and supported an allogeneic mixed leukocyte reaction . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , these cells express the accessory molecules CD 80 and CD 54 , while they lack constitutive expression of CD 86 and CD 40 , providing them the means to function as antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The use of green fluorescent protein labelled parasites allowed us to infect IL 10+ / + and IL 10 / mice and use a fluorescence activated cell sorter to distinguish infected and uninfected populations of macrophages and compare their expression of CD 80 , CD 86 and major histocompatibility complex ( MHC ) class 2 . ^^^ Taken together , these results indicate that IL 10 is not required for the virulence of the RH strain of T . gondii , nor is it involved in the regulation of the CD 80 , CD 86 and MHC class 2 molecules during RH infection . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
One approach is to genetically manipulate tumor cells to either secrete lymphokines ( GM CSF , IL 12 , IL 15 ) or express membrane bound molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This diminished T cell activation activity is due , in part , to dramatically reduced expression of the costimulation ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^ The restored T cell stimulation activity was directly correlated with induced and increased levels of CD 80 ( 16 fold ) and CD 86 ( 10 fold ) expression by the lymphokine cultured DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NK 92 also expresses other molecules implicated immune effector cell regulation ( CD 80 , CD 86 , CD40L , TRANCE ) whose relevance in NK killing is unclear . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Addition of anti CD 80 and anti CD 86 monoclonal antibody to cell cultures caused significant suppression of cell activation by antigenic stimulation . ^^^ Addition of RXM at a concentration of 5 . 0 micrograms / ml into cell cultures remarkably suppressed co stimulatory molecule , CD 40 , CD 80 and CD 86 , expressions , which enhanced by antigenic stimulation in vitro . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Surface expression of CD1d but not CD 40 , CD 80 , or CD 86 costimulator molecules was detected in hepatocytes . ^^^ Liver dendritic cells ( DC ) isolated from nontreated mice showed low surface expression of MHC , CD1d , and CD 40 , CD 80 , or CD 86 costimulator molecules that were strikingly up regulated after alphaGalCer injection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The small fraction of cells surviving in such cultures displayed a mature DC phenotype with expression of CD 83 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In normal intestinal mucosa surface expression of typical macrophage markers such as CD 14 , CD 16 , CD11b or T cell co stimulatory molecules such as CD 80 or CD 86 is low indicating anergy and low pro inflammatory activity of these cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The prominent ( 2 / 3 ) one is CD4+ CD45RA+ CD 33 CD11c , mostly immature as it lacks CD 80 , CD 83 and CD 86 , and is HLA DRint . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We analyzed intracellular pathways modulating surface densities of CD 80 and CD 86 in B cells activated through ligation of the Ag receptor , and the adhesion molecule CD 54 . ^^^ Whereas B cell Ag receptor ( BCR ) cross linking alone stimulated increased expression of CD 86 , up regulation of CD 80 required dual stimulation with anti IgM and anti CD 54 . ^^^ The principal downstream component contributed by BCR signaling , toward both CD 80 and CD 86 induction , was the elevated concentration of free cytoplasmic Ca ( 2+ ) , recruited by way of capacitative influx . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IFN beta in vitro also upregulated CD 80 and , in particular , CD 86 expression on DCs . ^^^ In vitro , anti CD 80 antibody remarkably increased , while anti CD 86 antibody inhibited DC induced IL 4 production in MLR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The DC expression of human leucocyte antigen ( HLA ) molecules , CD 80 , CD 83 , and CD 86 was markedly enhanced by exposure to huCD40LT even compared to TNF alpha exposure . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Thus , in contrast to DC pulsed with the Th 1 response inducing Ag Propionebacterium acnes , SEA exposed DC exhibit a phenotype that is most similar to that of immature DC , failing to up regulate expression of CD 40 , CD 54 , CD 80 , CD 86 , or OX40L ; producing no detectable IL 4 , IL 10 , or IL 12 ; and displaying only a minor increase in MHC class 2 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present report , we present findings on the gene sequences encoding the nonhuman primate homologues of human CD 80 , CD 86 , their ligands CD 28 and CD 152 , CD 154 , CD 95 , and CD 95 L from rhesus macaques and for phylogenetic analysis from pig tailed macaques , African sooty mangabey monkeys , baboons , and vervets as well as select molecules from the New World aotus and marmoset monkeys . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules such as CD 80 and CD 86 expressed by DCs , the polarizing cytokine environment during DC T cell interaction , and also the nature of the antigen are critical in the orientation of the immune response . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Novel transcripts encoding secreted forms of feline CD 80 and CD 86 costimulatory molecules . ^^^ Engagement of costimulatory molecules such as CD 28 or CD 152 ( CTLA 4 ) on T cells by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) dictates the nature of T cell mediated immune responses . ^^^ We previously reported the discovery of naturally occurring forms of canine CD 80 and CD 86 mRNAs which encode secreted CD 80 and CD 86 molecules . ^^^ We report here that mRNAs for secreted forms of CD 80 and CD 86 are also expressed in cats . ^^^ The detection of naturally occurring mRNAs encoding secreted CD 80 and CD 86 adds further complexity to the regulation of the B 7 CD28 / CD152 costimulatory pathway . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , expression of IL 18 and the B 7 proteins CD 80 and CD 86 are unchanged or increased compared to controls after induction of LPS tolerance . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This paralleled the induction by OMPC of CD 86 , CD 80 and CD 40 costimulatory molecules on human neonatal and murine B cells and of Th 1 cytokines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interference with CD 28 , CD 80 , CD 86 or CD 152 in collagen induced arthritis . ^^^ We have investigated interference with co stimulation by administering mAbs towards CD 28 , CD 80 , CD 86 , and CD 152 in mice immunized for the development of collagen induced arthritis ( CIA ) . ^^^ Anti CD 80 and anti CD 86 treatment inhibited disease score and incidence , whereas anti CD 28 treatment led only to a delayed disease onset . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Peritransplant macrophages and DCs from tolerant mice express down regulated CD 40 , CD 80 , and CD 86 costimulatory molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Exposure of DCs during maturation to tacrolimus or CsA resulted in no significant change in the expression of DC phenotypic markers , including CD 80 , CD 86 , and HLA Class 1 and 2 antigens determined by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BACKGROUND : B 7 molecules ( CD 80 , CD 86 ) and their counter receptors , CD 28 and CD 152 ( CTLA 4 ) , play an important role in T cell mediated immune responses . ^^^ However , following the nasal provocation with house dust , not only CD 86 , but also CD 80 , CD 28 , and CD 152 were significantly expressed in allergic patients . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that Murabutide triggers immunophenotypic changes as upon treatment , iDCs up regulate the surface expression of the major histocompatibility complex type 2 molecule human leucocyte antigen DR , the co stimulatory molecules CD 80 , CD 86 and CD 40 and the differentiation marker CD 83 , and down regulate the expression of the mannose receptor . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Eosinophils from the allergic lung expressed class 2 MHC peptides , T cell costimulatory molecules ( CD 80 and CD 86 ) , and rapidly internalized and processed Ag that was sampled from within the airway lumen . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Induction therapy with monoclonal antibodies specific for CD 80 and CD 86 delays the onset of acute renal allograft rejection in non human primates . ^^^ CD 80 and CD 86 ( also known as B 7 1 and B 7 2 , respectively ) are both ligands for the T cell costimulatory receptors CD 28 and CD 152 . ^^^ Both CD 80 and CD 86 mediate T cell costimulation , and as such , have been studied for their role in promoting allograft rejection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Inhibition studies using the B 7 binding fusion protein CTLA4Ig and antibodies against CD 80 and CD 86 demonstrate that CTLA4Ig and anti CD 86 inhibited influenza specific T cell proliferation , interleukin ( IL ) 2 and interferon ( IFN ) gamma production , and generation of influenza specific CD8+ CTL . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Thus cultivated cells escaped from apoptosis , and protein synthesis was induced , notably of the major histocompatibility complex ( MHC ) class 2 antigens , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Their phenotypes ( CD 14 , CD1a , CD 83 , HLA DR , CD 80 , CD 86 and CD 40 expression ) were examined by fluorescence activated cell sorting , and their T cell stimulatory potential was investigated in allogeneic mixed lymphocyte reaction ( allo MLR ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Monocytes cultured for 7 days in 10 VIVO 15 serum free media in the presence of GM CSF + IL 4 showed down regulation of CD 14 with increased expression of HLA DR , mannose receptor , CD 80 , and CD 86 , along with highly up regulated CD1a ( + ) expression . ^^^ The addition of autologous serum to serum free media in monocyte cultures resulted in a dose dependent decrease in the CD1a ( + ) expression generating a distinct subset of CD1a ( + / ) cells expressing HLA DR , mannose receptor , CD 80 , and CD 86 . ^^^ CD1a ( + ) and CD 14 ( + ) cells expressing HLA DR , mannose receptor , CD 80 , and CD 86 were generated in 7 days from CD 34 ( + ) cells in serum free media . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , upon contact with C . pneumoniae dendritic cells were potently stimulated because NF kappaB was activated and translocated to the nucleus , cytokines like IL 12p40 and TNF alpha were secreted , and expression of MHC class 2 molecules , CD 40 , CD 80 , and CD 86 was up regulated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The co stimulatory molecules CD 80 and CD 86 were examined by direct immunofluorescence for cell surface expression and RT PCR amplification for mRNA . ^^^ CD 80 protein was not detected at all , while an insignificant percentage of cells were positive ( mean 2 % ) for CD 86 in all cell lines tested . gamma IFN had no apparent effect on CD 80 or CD 86 protein expression . ^^^ Constitutive CD 80 or CD 86 mRNA levels were observed in 45 and 61 % of the NSCLC lines , respectively . ^^^ Overall , gamma IFN stimulation led to the up regulation of MHC Class 1 molecules and class 2 molecules as well as CD 80 and CD 86 mRNA transcripts . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The MAC DC stimulatory capacity was enhanced by LPS , although the expression of HLA class 2 , CD 83 , CD 80 and CD 86 was unmodified . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The association of molecules like CD 80 , CD 86 ( co stimulatory molecules ) in monocytes and B cells and CD 30 , CD62L , ALL , CD11a , CD 28 , CD 124 and CD 152 in CD4+ , they have shown to be of particular interest in allergic sufferings . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , we show that carcinoma cells upregulate expression of CD 54 and MHC molecules following in vitro exposure to srhCD40L but do not upregulate CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
ICOS binds to a newly identified ligand on antigen presenting cells different from the CD 152 ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Coadministration of either cyclosporine or steroids with humanized monoclonal antibodies against CD 80 and CD 86 successfully prolong allograft survival after life supporting renal transplantation in cynomolgus monkeys . ^^^ BACKGROUND : Recent studies have shown some efficacy using monotherapy with monoclonal antibodies ( mAb ) against CD 80 and CD 86 receptors after life supporting renal transplantation in non human primates . ^^^ All monkeys in groups 1 , 2 , and 4 were treated with the combination of a CD 80 ( h1F1 ) and CD 86 ( h3D1 ) mAb given at 20 mg / kg each preoperatively , then 5 mg / kg at weekly intervals starting postoperative ( po ) day 0 until poday 56 ( 9 doses ) . ^^^ In addition , combining CsA with mAbs directed against the CD 80 and CD 86 receptors significantly prolongs graft survival when compared to CsA monotherapy . ^^^ Therefore clinical trials of humanized mAbs to CD 80 and CD 86 used in combination with conventional immunosuppression can be considered . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To elucidate a role of costimulatory molecule and cell adhesion molecule in hepatic ischemia / reperfusion injury , we examined an alteration in B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) expression in the rat liver after warm ischemia / reperfusion injury . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of the co stimulatory molecules CD 80 and CD 86 was increased and the majority of these cells retained their expression of CD 34 ( 73+ / 4 % ) and HLA DR ( 79+ / 5 % ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After human monocytes were cultured for 24 hours in serum free medium and granulocyte macrophage colony stimulating factor to produce immature DCs , additional overnight culture with either calcium ionophore ( CI ) or interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and soluble CD40L resulted in phenotypically mature DCs that produced interleukin 8 ( IL 8 ) and displayed marked expression of CD 80 , CD 86 , CD 40 , CD 54 , CD 83 , DC LAMP , and RelB . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
AF did not influence the cell surface expression of the class 2 MHC molecule and the costimulatory molecules CD 80 and CD 86 . 3 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Second , specific activation of dendritic cells was indicated by their marked up regulation in expression of costimulatory molecules B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , and ICAM 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that the synthetic PPARgamma agonist rosiglitazone ( as well as pioglitazone and troglitazone ) significantly increases on LPS and CD40L activated MDDC , the surface expression of CD 36 ( by 184 % and 104 % , respectively ) and CD 86 ( by 54 % and 48 % ) , whereas it reduces the synthesis of CD 80 ( by 42 % and 42 % ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Incubation of dendritic cells with P . c . chabaudi schizonts also resulted in up regulation of MHC class 2 , CD 40 and CD 86 but not CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In PDC , CpG ODN supported survival , activation ( CD 80 , CD 86 , CD 40 , MHC class 2 ) , chemokine production ( IL 8 , IP 10 ) and maturation ( CD 83 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Sentinel nodes ( SNs ) and non SNs were evaluated by routine pathologic analysis , and a portion of each node was processed for expression of three dendritic markers of activation ( CD 80 , CD 86 , CD 40 ) and their corresponding T cell receptors ( CTLA 4 and CD 28 ) . ^^^ Reverse transcriptase polymerase chain reaction ( RT PCR ) analyses of paired SNs and non SNs demonstrated a marked reduction in semiquantitative expression of CD 80 ( 77 % ) , CD 86 ( 77 % ) , and CD 40 ( 85 % ) , as well as CTLA 4 ( 88 % ) and CD 28 ( 85 % ) in SNs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Whereas most human DC populations uniformly express some costimulatory molecules such as B 7 2 ( CD 86 ) , previous studies have shown a wide variation in the expression of B 7 1 ( CD 80 ) among different human DC preparations . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The distribution patterns of DCs and their major costimulatory molecules , CD 80 , CD 86 and CD 40 in the spleen were identified , and the evidence for maturation of DCs in vivo in response to SEB was obtained . ^^^ This movement paralleled a maturation process , as assessed by upregulation of CD 40 , CD 80 and CD 86 expression in the interdigitating dendritic cells ( IDCs ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The phenotype of dendritic cells was determined with antibodies to CD45RC , CD62L , and the maturation markers CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ CD62L , CD 80 , and CD 86 were lower on CD45RC ( ) OX 62 ( + ) than CD45RC ( + ) OX 62 ( + ) cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The presence of M . furfur induced maturation ( CD 83 expression ) of MDDCs , and up regulation of the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both molecules bind to the same ligands , B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) but have antagonistic functions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 is a T cell surface molecule that binds to the costimulatory molecules CD 80 and CD 86 on antigen presenting cells and downregulates T cell function . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In portal infiltrates , cells with electron microscopical and cell marker features of dendritic cells and expressing the activation markers CD 54 , CD 80 , CD 83 and CD 86 were organized in a discontinuous network , that embedded CD8+ lymphocytes in close contact with dendritic cells and came in contact with hepatocytes , sometimes infiltrating beyond the limiting plate . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Further analysis of cell surface antigens such as CD 80 , CD 86 , HLA DR , CD11a , CD 14 , CD 23 , CD 25 and CD 54 revealed that in the course of cutaneous sensitization the phenotype of the dendritic cells was not altered in the afferent lymph . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis indicated enhanced expression of MHC class 1 and 2 molecules as well as CD 80 , CD 86 , CD 40 , and CD 54 , and reverse transcription PCR analysis revealed increased levels of interleukin 12 p 40 mRNA . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of cell surface molecules CD 40 , CD 69 , CD 80 , CD 86 , CD 95 and HLA DR was measured by flow cytometry . ^^^ MNC exposed to IFN beta in vitro enhanced expression of the co stimulatory CD 80 , CD 86 , the early activation antigen CD 69 and the cell death receptor CD 95 . ^^^ In conclusion , anti inflammatory effects of IFN beta on blood MNC include augmentation of IL 10 production and suppression of IL 12 p 40 production , which are accompanied by enhancement of CD 69 , CD 80 , CD 86 and CD 95 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) do not have distinct roles in setting the Th1 / Th2 balance in autoimmunity in rats . ^^^ Some data suggest that the interaction between CD 28 and CD 80 ( B7 . 1 ) stimulates Th 1 responses and that CD 28 and CD 86 ( B7 . 2 ) stimulates Th 2 responses , however this is controversial . ^^^ Monoclonal antibodies ( MoAbs ) to CD 80 and CD 86 , given singly , had little effect in either model , however when given together they almost completely suppressed the HgCl 2 induced autoimmunity : the peak immunoglobulin ( Ig ) E concentration was 3 . 25 microg / ml in treated animals versus 2770 microg / ml in controls ( P < 0 . 0001 ) ; caecal vasculitis was suppressed with a median vasculitis score of 0 in treated animals versus 6 in controls ( P < 0 . 0001 ) ; and new germinal centre formation was significantly suppressed . ^^^ We have demonstrated profound suppression of both Th 1 and Th 2 driven autoimmunity in rats by a combination of anti CD 80 and CD 86 , but have been unable to demonstrate any clear differential effects . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They expressed CD 4 , CD11a , CD11b , CD11c , CD 18 , CD 32 , CD 33 , CD45R0 , CD 48 , CD 50 , CD 54 , CD 58 , CD 64 , CD 86 , CD 102 , CD 116 , CD 123 , and HLA DR ; showed no expression of CD1a and CD 83 ; and weak expression of CD 16 , CD45RA , and CD 80 . ^^^ The levels of CD 80 and CD 86 expression were comparable ; however , in contrast to G AP monocytes , CB monocytes lacked CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of the costimulatory molecules CD 80 and CD 86 on DCs from these patients ( mean , 29 % + / 17 % of MNCs and 85 % + / 10 % of MNCs , respectively ) was also normal ( mean , 29 % + / 17 % and 86 % + / 16 % of MNCs , respectively ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that MDP Lys markedly up regulated the expression of CD 80 , CD 83 , CD 86 , and CD 40 , but not human leukocyte antigen DR , and stimulated the production of tumor necrosis factor alpha , IL 6 , IL 8 , IL 10 , and IL 12 ( p 40 ) by human DCs in a dose dependent manner . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Langerhans ' cells in vernal keratoconjunctivitis express the costimulatory molecule B 7 2 ( CD 86 ) , but not B 7 1 ( CD 80 ) . ^^^ The objective of this study was to examine the expression of costimulatory molecules CD 28 , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in conjunctival biopsies from patients with active VKC and normal controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CLL DC had a similar expression of accessory molecules ( CD 54 , CD 80 and CD 86 ) as control DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The following molecules were studied : the co stimulatory molecules CD 80 , CD 86 and CD 40 ; the adhesion molecules CD11a c , CD 31 , CD 43 , CD 50 , CD 54 , CD 102 , CD 58 and CD62L ; the chemokine receptor CXCR 4 ; and the death receptors TNFR 1 and TNFR 2 and FAS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The delayed addition of IL 4 on day 6 of culture gave rise to an additional increase in the yield of CD1a+CD14 DCs that were characterized by the expression of HLA ABC , HLA DR , CD 80 , CD 86 , and CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Distinct role of CD 80 and CD 86 in the regulation of the activation of B cell and B cell lymphoma . ^^^ To date , not much has been known regarding the role of CD 80 and CD 86 molecules in signaling of B cells . ^^^ The CD28 / CTLA4 ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , are expressed on the surface of freshly isolated splenic B cells , and their expression is up regulated by lipopolysaccharides . ^^^ In contrast , GL 1 augmented the level of anti apoptotic molecules Bcl w and Bcl 10 ( L ) and decreased the levels of pro apoptotic molecule caspase 8 , thereby providing a novel insight into the mechanism whereby triggering through CD 80 and CD 86 could deliver regulatory signals . ^^^ Thus , this study is the first demonstration of a distinct signaling event induced by CD 80 and CD 86 molecules in B cell lymphoma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential role of CD 80 and CD 86 molecules in the induction and the effector phases of allergic rhinitis in mice . ^^^ Selective involvement of CD 80 and / or CD 86 in the differentiation of T helper ( Th ) 1 and Th 2 was seen in several diseases . ^^^ In this study , we sought to determine the differential roles of CD 80 and CD 86 in the induction and effector phase of allergic rhinitis using Schistosoma mansoni egg antigen ( SEA ) as a specific Ag . ^^^ Blockade of CD 80 at the induction phase significantly inhibited these manifestations , whereas no effect was observed by CD 86 blockade . ^^^ In contrast , the simultaneous blockade of both CD 80 and CD 86 during the effector phase partially inhibited IgE and IgG ( 1 ) production and nasal eosinophilia , although either blockade of CD 80 or CD 86 during the phase failed to inhibit these responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression pattern of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , adhesion molecules ( CD 54 ) , and intracellular cytokines ( interleukin 12p70 and tumor necrosis factor [ TNF ] alpha ) in DC after interaction with endothelial cells was determined by immunofluorescence . ^^^ Adhesion of DC to PAEC also resulted in the activation of DC , which was manifested by up regulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , adhesion molecules ( CD 54 ) , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
By genetic targeting of gp 96 onto the cell surface , we demonstrate that direct access of gp 96 to DCs induces their maturation , resulting in secretion of proinflammatory cytokines IL 1beta , IL 12 , and chemokine monocyte chemoattractant protein 1 and up regulation of the expression of MHC class 1 , MHC class 2 , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Optimal T cell activation by antigen presenting cells requires co stimulatory signaling , such as the interaction of CD 80 , CD 86 , or both with CD 28 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upon terminal differentiation with LPS or CD 40 ligand , the acquisition of a mature surface phenotype was variably achieved among WAS patients , with increased expression of CD 80 , CD 86 and DC LAMP . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After 11 days of co culture , CD 34 ( + ) derived cells display a non adherent undifferentiated morphology , a high level of monocytic CD 14 marker , a down regulated expression of LC markers ( CD1a , E cadherin ) and DC markers ( CD 40 , CD 80 , CD 54 , CD 58 , CD 83 , CD 86 , HLA DR , HLA class 1 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interestingly , these cells have elevated levels of MHC class 2 and CD 95 , but not CD 40 , CD 80 , or CD 86 , suggesting that they are poised to undergo deletion rather than activation upon T cell encounter . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BACKGROUND : Constitutive expression of major histocompatibility complex ( MHC ) class 2 antigens and of the co stimulatory receptors CD 80 and CD 86 is restricted to professional antigen presenting cells . ^^^ METHODS : To continue and extend the previous study results , PMN and monocytes of 60 patients with Wegener ' s granulomatosis , 24 patients with microscopic polyangiitis ( MPA ) , 20 patients with acute bacterial infection , and 53 healthy donors were analyzed for the expression of MHC class 2 antigens as well as of CD 80 and CD 86 . ^^^ RESULTS : PMN of patients with acute , active Wegener ' s granulomatosis expressed MHC class 2 antigens , CD 80 and CD 86 ; on monocytes up regulation of MHC class 2 was seen . ^^^ In contrast , PMN of patients with inactive disease , or with relapse , patients with microscopic polyangiitis or with bacterial infections expressed neither MHC class 2 , nor CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cell fraction of DC / BNL fusions showed phenotypes of DCs , MHC class 2 , CD 80 , CD 86 , and intercellular adhesion molecule ( ICAM ) 1 , which were not expressed on BNL cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In situ expression of the costimulatory molecules CD 80 and CD 86 on langerhans cells and inflammatory dendritic epidermal cells ( IDEC ) in atopic dermatitis . ^^^ Flow cytometry , skin mixed lymphocyte reactions ( SMLR ) and immunohistological analysis were performed , and showed that IDEC and not LC are the relevant cells expressing the costimulatory molecules CD 80 and CD 86 in situ . ^^^ This expression varied with the underlying diagnosis , with AD showing the highest expression of both CD 80 and CD 86 in situ . ^^^ Furthermore , the expression of CD 80 , CD 86 and CD 36 were significantly correlated . ^^^ With short term culture , both CD 80 and CD 86 were further upregulated on LC and IDEC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Maturation parameters such as production of IL 12 and increases in cell surface expression of HLA DR , CD 80 , CD 86 , CD 40 , and CD 83 were observed following DC treatment with MPL . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Infection induced DC morphology and altered the expression of surface markers , including loss of CD 14 , de novo induction of CD 83 and CD 25 , and strongly augmented expression of CD 86 , CD 80 , CD 40 , and HLA DR and HLA class 1 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Reduced accessory cell function was not due to changes in surface expression of monocyte costimulatory molecules ( HLA class 1 , HLA DR , CD 80 , CD 86 , CD 40 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
FACS analysis of the DC populations revealed that AAV / E6 vector pulsed DCs had higher levels of CD 80 and lower levels of CD 86 than protein pulsed DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immunophenotypic analysis confirmed that all stimuli induced stable up regulation of CD 25 , CD 40 , CD 80 , CD 83 , CD 86 , HLA DR , DC LAMP ( CD 208 ) , and DEC 205 ( CD 205 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , signal 2 is elicited by interaction between CD 28 and its ligands ( CD 80 and CD 86 ) and is antigen independent . ^^^ Interestingly , cell surface expression of CD 80 and CD 86 on antigen presenting cell ( APC ) is regulated by stimulus via CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Comparing with CD1a DCs developed without heparin , CD1a+ DCs express higher CD 40 and CD 80 and lower CD 86 . ^^^ CD 80 , CD 86 , HLA DR , and CD 40 are proportionally up regulated when both types of DCs are stimulated with LPS or LPS plus IFN gamma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IFN gamma and IL 4 levels in the supernatant of BLAF lymphocytes cultured with PHA were determined using sandwich ELISA and the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expressed by AM stimulated with LPS were determined using avdin biotin complex technique . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NLCs also express CD 14 , as well as CD11b , CD 33 , CD 40 , CD45RO , CD 68 , CD 80 , CD 86 , HLA DQ , and HLA DR , but not CD1a , CD 2 , CD 3 , CD11c , CD 19 , CD45RA , CD 83 , CD 106 , or CD 154 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blocking the CD 80 and CD 86 costimulation molecules : lessons to be learned from animal models . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The effects of IPD 1151T on the expression of co stimulatory molecules , CD 40 , CD 80 and CD 86 , were investigated in vivo using mice with allergic disorders . ^^^ IPD 1151T caused suppression of CD 40 and CD 86 expression ; however , the treatments did not affect CD 80 expression . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both cell lines failed to express CD 80 , CD 86 , CD 40 , or CD 48 in vitro and in a mixed lymphocyte reaction were unable to induce T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In particular , the expression levels of HLA DR , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on DC surface from patients were also lower than that from healthy individuals ( 0 . 46 vs 0 . 92 for HLA DR , 0 . 44 vs 0 . 88 for CD 80 and 0 . 44 vs 0 . 84 for CD 86 , P < 0 . 05 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : DC cells isolated and generated were CD11c+ cells with irregular shape , and highly expressed CD 80 , CD 86 and CD 54 molecules . ^^^ H 22 cells were CD11c cells with spherical shape and bigger volume , and did not express CD 80 , CD 86 and CD 54 molecules . ^^^ H ( 22 ) DC was CD11c+ cells with bigger volume , being spherical , flat or irregular in shape , and highly expressed CD 80 , CD 86 and CD 54 molecules , too . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : CD 86 was expressed in 54 % AML , whereas CD 80 and CD1a were only occasionally positive . ^^^ Four samples enriched in CD 34 ( + ) CD 86 ( + ) AML cells differentiated into AML DC CD 86 ( + ) , CD 80 ( + ) , CD 40 ( + ) , CD11c ( + ) , HLA DR ( ++ ) , CD 14 ( + / ) that also were CD1a ( + ) or CD 83 ( + ) , after 6 days of in vitro culture with GM CSF + / TNF alpha . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A portion of each node was processed for expression of the dendritic markers of activation CD 80 , CD 86 , and CD 40 , and their corresponding T cell receptors CTLA 4 and CD 28 . ^^^ Reverse transcription polymerase chain reaction analyses of corresponding sections of the sentinel and nonsentinel nodes demonstrated a marked reduction in semiquantitative expression of CD 80 ( 77 % ) , CD 86 ( 77 % ) , and CD 40 ( 85 % ) , as well as CTLA 4 ( 88 % ) and CD 28 ( 85 % ) in sentinel as compared to nonsentinel nodes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , CD 86 expression was significantly lower on fusion positive samples ( P = 0 . 010 ) while there was no difference in expression of CD 80 or major histocompatibility complex class 1 between ETV 6 AML1 positive and negative samples . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We demonstrate that extracellular matrix ( ECM ) , hyaluronic acid ( HA ) and chondroitin sulphate A ( CSA ) , in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , can rapidly promote the differentiation of monocyte derived immature DCs , as characterized by the remarkable upregulation of human leucocyte antigen ( HLA DR ) , CD 40 , CD 54 , CD 80 and CD 86 expression to levels higher than those in the DCs generated by culturing with GM CSF and interleukin ( IL ) 4 for 7 days and aggregation of the cells within 48 h . ^^^ The upregulation of expression of HLA DR , CD 40 , CD 54 , CD 80 and CD 86 was dose dependent . ^^^ Inhibition of NF kappaB by pyrolidine dithiocarbamate and sodium salicylate , and serine threonine and tyrosine kinase by starosporine as well as phosphatidylinositide 3 kinase ( PI 3 K ) by wortmannin could prevent the effects of HA and CSA on the expression of HLA DR , CD 40 , CD 80 and CD 86 in various degrees . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 interacts with the ligands CD 80 and CD 86 on antigen presenting cells ( APC ) , and also directs the assembly of inhibitory signalling complexes that lead to quiescence or anergy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The B and T cell populations residing within the lymph follicles and / or within B cell type MALT lymphoma were characteriZed by an immunohistochemical technique , utilizing B and T cell markers including : CD 3 , CD 4 , CD 8 ( T cells ) ; CD 20 , CD 40 , GD 74 , BLA 36 , CD 80 , CD 86 ( B cells ) . ^^^ The B cell markers , CD 80 and CD 86 , were found within the centrocytic zone of the lymph follicle . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendritic cells were abundant in the lamina propria of pocket epithelium ; they were MHC 2 positive , admixed with CD 4 positive and CD 8 positive T lymphocytes , and , they expressed CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The capacity of this T cell subset to be directly stimulated by nickel was not due to unique antigen presenting properties , as both antigen presenting cell dependent and antigen presenting cell independent clones displayed comparable levels of HLA DR , CD 80 , and CD 86 , and were equally capable of presenting nickel to antigen presenting cell independent clones . ^^^ T T presentation induced T cell receptor downregulation , CD 25 , CD 80 , CD 86 , and HLA DR upregulation , and interferon gamma release , although to a lesser extent compared to those induced by dendritic cell T presentation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of MHC class 1 and class 2 , Intercellular cell adhesion molecule ( ICAM 1 ) , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) molecules was quantitatively analyzed by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our data show that transfection of DCs with recombinant adenovirus AdV CD40L resulted in activation of DCs with up regulated expression of proinflammatory cytokines ( IL 1beta and IL 12 ) , chemokines ( RANTES , IP 10 , and MIP 1alpha ) , and immunologically important cell surface molecules ( CD 54 , CD 80 , and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , the co stimulatory molecules CD 80 and CD 86 and the dendritic cell markers CD1a and CD 83 were scarce in SpA synovium . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
By the treatment of two step cultured cells with calcium ionophore agent ( A 23187 ) , the expression of co stimulatory molecules such as CD 86 and CD 80 ( especially CD 86 ) was up regulated . ^^^ Consistent with the up regulation of surface molecules CD 86 , CD 80 and CD 83 , the two step cultured cells treated with A 23187 also showed a stronger allo stimulatory capacity compared with the cells without A 23187 treatment . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
First , thanks to the innate immune system ' s regulation of the main costimulatory molecules CD 80 and CD 86 , the immune system rarely mistakes a pathogen for a self antigen . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A primary costimulatory signal is delivered through the CD 28 receptor after engagement of its ligands , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This was sequentially followed by the up regulation of CD 86 , CD 80 , CD 40 , and human leukocyte antigen DR expression on CD 14 ( + ) monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : Normal and inflamed corneas were excised from BALB / c mice , and immunofluorescence staining for CD11c , CD11b , CD 45 , CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , CD 3 , and MHC class 2 ( Ia ) was performed by confocal microscopy on wholemount corneal epithelium . ^^^ Additionally , these cells were CD 80 and CD 86 negative , reflecting an immature phenotype . ^^^ In the inflamed cornea , the expression of MHC class 2 and costimulatory molecules CD 80 and CD 86 was significantly enhanced , and present in all parts of the cornea , in contrast to the normal cornea . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we compared the effect of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on the induction of T cell immunity to C 1498 , a murine myelogenous leukemia . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Inhibition of murine corneal allograft rejection by treatment with antibodies to CD 80 and CD 86 . ^^^ The purpose of this study was to investigate the role of CD 80 and CD 86 costimulatory molecules in corneal allograft rejection . ^^^ Anti CD 80 and anti CD 86 monoclonal antibodies ( mAbs ) were administered after orthotopic corneal allograft transplantation . ^^^ Population and localization of CD 80 ( + ) and CD 86 ( + ) cells in the cornea , cervical lymph nodes , and spleen were examined by flow cytometry and immunohistochemistry . ^^^ The combined use of anti CD 80 and anti CD 86 mAbs was effective in prolonging corneal allograft survival . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules on antigen presenting cells play important roles in providing co stimulatory signals required for activation and expansion of autoreactive T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Following treatment with LPS in vivo , all DC subsets upregulated CD 40 , CD 80 , CD 86 , and MHC class 2 expression , but surprisingly Ag capture was not downregulated and some DC subsets retained expression of intracellular MHC class 2 vesicles . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypically , the expression of CD 40 , CD 80 , CD 86 and major histocompatibility complex class 2 antigen on the LCs was upregulated on the fifth and seventh day . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mature DCs expressed significantly heightened levels of their antigen presenting machinery ( e . g . , CD 54 , CD 80 , CD 86 ) and numerous cytokines and chemokines / chemokine receptors ( i . e . , Flt 3L , G CSF , IL 1alpha and 1beta , IL 6 , IL 12 , CCL 2 , 3 , 4 , 5 , 17 , and 22 , MIP 2 , and CCR 7 ) and were significantly better at inducing effector T cell responses in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cytokine production from blood cells was determined , and cell surface B 7 ( CD 80 , CD 86 ) expression was measured . ^^^ Incubation of blood cells with IL 6 resulted in increased expression of surface CD 80 and CD 86 , while IL 10 decreased CD 80 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The small number of mature B cells that do develop in Lyn deficient mice express normal levels of the transgenic BCR and lack expression of CD 80 and CD 86 , suggesting they are not activated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although the precise function of CD 83 is not known , its selective expression and upregulation together with the costimulators CD 80 and CD 86 suggests an important role of CD 83 in the induction of immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These CD1a+ cells lacked the expression of CD 80 , CD 86 , or CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : Kinetics of CD 80 and CD 86 expression in the cornea and draining lymph nodes were examined by RT PCR and immunohistochemistry in untreated allograft recipients in a high responder rat model . ^^^ RESULTS : Expression of CD 80 mRNA was increased in both donor and recipient cornea 16 hours after transplantation , whereas CD 86 was detected constitutively , with no significant early increase . ^^^ Immunohistochemistry on day 5 after transplantation demonstrated major histocompatibility complex ( MHC ) class 2 expression , no CD 80 , and only a trace of CD 86 in corneal allografts . ^^^ In lymph nodes strong MHC class 2 , weak CD 80 , and moderate CD 86 expression was noted . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This viability was accompanied by progressive up regulation of the surface costimulatory ( CD 40 , CD 80 , CD 86 ) and activation ( CMRF 44 , CMRF 56 , CD 83 ) molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They up regulated the expression of HLA DR , CD 80 , CD 86 , partially CCR 7 but not CD 83 , partially reduced antigen uptake function , increased the levels of IL 12p35 mRNA , and prolonged surface expression of peptide MHC class 1 complexes for presentation to cytotoxic T lymphocytes , but did not induce migration towards CCL 21 chemokine . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The observed expression patterns of CD 80 and CD 86 on different APC ( B cells in type 2 and dendritic cells in type 1 ) may be responsible for this discrepancy . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vivo CD 86 blockade inhibits CD4+ T cell activation , whereas CD 80 blockade potentiates CD8+ T cell activation and CTL effector function . ^^^ To address whether a functional dichotomy exists between CD 80 and CD 86 in naive T cell activation in vivo , we administered anti CD 80 or CD 86 blocking mAb alone or in combination to mice with parent into F ( 1 ) graft vs host disease ( GVHD ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cell surface expression of the costimulatory molecules CD 80 and CD 86 was not reduced by the preincubation procedure , and only a moderate reduction of IL 6 production was observed . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We hypothesize that expression of CD 80 and / or CD 86 on porcine cells may also play a role in NK cell activation as human NK cells express a variant of CD 28 . ^^^ Our initial analysis showed that porcine endothelial cells and fibroblasts express CD 86 , but not CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Similar expression of CD1a , CD 80 , CD 86 and CD 83 were observed in both ISO DC and AD DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present study , we investigated the ability of two selected APC populations , the dendritic cells ( DCs ) highly expressing CD 80 and CD 86 molecules ( CD80highCD86high ) and the monocytes expressing the same molecules at a rather low level ( CD80lowCD86low ) , to stimulate the proliferation of purified bovine WC1+gammadelta T cells to SAgs . ^^^ Finally , the addition of monoclonal antibody ( MoAb ) anti CD 86 markedly inhibited SAgs and ConA mediated proliferation , whereas MoAb anti CD 80 had no effect . ^^^ The combination of both anti CD 80 and anti CD 86 , however , suppressed this response . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These molecules include CD1a , HLA DR , CD 40 , CD 83 , CXCR 4 , and to a lower extent CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These studies also revealed that DC that matured in response to T cell mitogens display , comparatively to LPS , a distinctive phenotype characterized by high expression of the MHC class 2 , CD 40 , and CD 205 markers , but only moderate ( CD 86 ) to minimal ( CD 80 ) expression of CD28 / CTLA4 ligands . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Surface expression of CD 25 , CD 28 , CD 40 , CD 54 , CD 80 , CD 86 and CTLA 4 was evaluated on CD 3 , CD 4 , CD 8 , CD 14 and CD 19 PBMC subpopulations by three colour flow cytometry . ^^^ Levels of CD 80 and CD 86 in the control cultures were lower in the asthmatic group . ^^^ RV infection induced a significant increase of CD 80 on monocytes and of CD 86 on B cells , which occurred in both groups but were less marked in atopic asthmatic subjects . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Accumulation of DC SIGN+CD40+ dendritic cells with reduced CD 80 and CD 86 expression in lymphoid tissue during acute HIV 1 infection . ^^^ The co stimulatory molecules CD 80 and CD 86 were , however , only partially upregulated and the complete parafollicular network found in acute EBV infection was not generated , despite increased expression of interleukins 1alpha , 1beta , 12 ; interleukin 1alpha receptor antagonist ; interferon alpha ; and CD 40 expression . ^^^ LTNP / SLP and treated aviremic subjects had increased frequency of interdigitating DC , albeit lower than in aHI , and low expression of CD 80 and CD 86 . ^^^ The infiltration of DC in LT in acute EBV infection was accompanied by upregulation of CD 80 and CD 86 expression , which did not occur in aHI . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fluorescence activated cell sorter analysis performed at an early stage of development ( day 6 ) revealed an immature phenotype with intermediate levels of major histocompatibility complex ( MHC ) class 2 expression and low levels of the costimulator molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We find that during the acute phases of Plasmodium yoelii erythrocyte stage infection , APC upregulate the expression of class 2 MHC and CD 80 , maintain expression of CD 86 , process and present antigen , and support gamma interferon production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) are functionally equivalent in the initiation and maintenance of CD4+ T cell proliferation after activation with suboptimal doses of PHA . ^^^ Many in vitro and in vivo studies demonstrated that both CD 80 and CD 86 ligands have an identical role in the activation of T cells . ^^^ Recently , functions of B 7 costimulatory molecules in vivo have been investigated in B 7 1 and / or B 7 2 knockout mice , and the authors concluded that CD 86 could be more important for initiating T cell responses , while CD 80 could be more significant for maintaining these immune responses . ^^^ In this study , we directly compared the role of CD 80 and CD 86 in initiating and maintaining proliferation of resting CD 4 ( + ) T cells in an in vitro mode system that allowed to provide the first signal to effector cells through the use of suboptimal doses of PHA and the second costimulatory signal through cells expressing CD 80 or CD 86 , but not any other costimulatory molecules . ^^^ Using this experimental system we demonstrate that the CD 80 and CD 86 molecules can substitute for each other in the initial activation of resting CD 4 ( + ) T cells and in the maintenance of their proliferative response . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This inhibition was mainly dependent upon the CD 86 pathway and to a lesser extent upon the CD 80 pathway . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Transient blocking of both B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) in addition to CD 40 CD40L interaction fully abrogates the immune response following systemic injection of adenovirus vector . ^^^ Since most previous studies have used a CTLA 4 Ig fusion molecule binding to both CD 80 and CD 86 , the respective roles of these B 7 molecules remained undefined . ^^^ We have studied the effect of blocking monoclonal Abs ( mAbs ) directed against the costimulatory molecules CD40L , CD 80 and CD 86 , alone or in different combinations , on the humoral and cellular immune responses against Ad . ^^^ Combinations of anti CD 80 + anti CD 86 or anti CD40L + anti CD 86 mAbs resulted in strong inhibition of the immune response against Ad . ^^^ However , only the blockade of both CD 80 and CD 86 in addition to CD40L fully inhibited the humoral and cellular responses against the Ad vector , such that readministration after 1 month was as efficient as in naive animals . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The immune response is triggered by presentation of antigen peptides on the major histocompatibility assembly of the antigen presenting cells ( APC ) with the assistance of costimulatory molecules such as B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After infection with AdvCD40L , A 20 tumor cells up regulate several T cell costimulatory molecules ( CD 80 , CD 86 , ICAM 1 , and LFA 3 ) and Fas expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both subsets expressed TLR 7 and could respond to TLR 7 ligands , which enhanced the survival of the subsets and upregulated the surface expression of costimulatory molecules such as CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The first one is an antigen dependent one via the T cell receptor , the second one is a costimulatory signal which can be delivered by the CD 28 molecule after binding to CD 80 ( B7 . 1 ) or CD 86 ( B7 . 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 expression is induced on activated B cells following stimulation by CD 86 . ^^^ We examined the effects of CD 86 ligation on surface densities of CD 80 on activated B cells . ^^^ Incubation of such CD 86 expressing B cells with anti CD 86 monoclonal antibody ( MoAb ) resulted in a marked upregulation in cell surface levels of CD 80 . ^^^ Such an enhancement in surface levels was also accompanied by a marked increase in CD 80 mRNA , suggesting that the effects of CD 86 triggering are exerted at the level of CD 80 message . ^^^ Furthermore , coculture of CD 86 upregulated B cells with CD 28 expressing fibroblasts also led to the enhancement of CD 80 levels , which was not obtained upon incubation with cells expressing cytotoxic T lymphocyte associated molecule ( CTLA ) 4 Ig . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells displayed a typical DC phenotype , expressing MHC class 2 , CD 54 , CD 80 , CD 86 , and CD11b / c . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The effect of inactivation of co stimulated particles B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) on T lymphocyte activity ] . ^^^ We have studied the effect of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) costimulatory molecules inactivation on IL 4 and IFN gamma production by T lymphocytes . ^^^ We have added anti CD 80 or anti CD 86 monoclonal antibodies to the monocyte / lymphocyte ( 1 : 5 ) cultures . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We also analyzed the expression of CD 80 , CD 86 , HLA DR , CD 40 , and CD 57 on the macrophage surface after stimulation with LPS in the presence or absence of T . cruzi GIPL . ^^^ Similarly , GIPL led to down modulation of CD 83 , CD 80 , CD 86 , and HLA DR surface expression and TNF alpha and IL 10 production when DC were stimulated by CD40L . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 6 inhibited the colony growth of CD 34 ( + ) DC progenitors and switched the commitment of CD 34 ( + ) cells from DCs to CD 14 ( + ) CD1a ( ) CD 86 ( ) CD 80 ( ) CD 40 ( + / ) HLA DR + / monocytic cells exerting potent phagocytic activity but no antigen presentation capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of the co stimulatory molecules CD 80 , CD 86 and CD 40 appeared similar for both DC populations and not to relate to differences in function . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 , expressed on the antigen presenting cells ( APCs ) provide costimulatory signals for T lymphocytes . ^^^ In contrast to CD 86 , expression of CD 80 on the CD14+ cells induced by GM CSF was reduced in SLE . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The major second signal is generated by interaction of the CD 28 molecule expressed on most T lymphocytes with its natural ligands CD 80 and CD 86 located on APCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Conversely , GM CSF treatment transformed the NKT cells into dendritic cells , inducing rapid expression of HLA DR and the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The effect of TGF beta 1 on Ag presentation was shown to be independent of the surface expression of CD 80 , CD 86 , or CD 40 costimulatory molecules by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We showed that bone marrow derived DC pulsed with Id CD40L upregulated the expression of CD 40 , CD 80 , CD 86 , and major histocompatibility complex ( MHC ) class 2 molecules with the increased production of interleukin 12 ( IL 12 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we analyzed the expression of co stimulatory molecules ( CD 80 and CD 86 ) and CD 83 , which is a marker of mature dendritic cells , on gingival cells that were isolated from severe periodontitis tissues , with the use of flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of MHC class 2 , CD 54 , CD 80 and CD 86 by prostatic dendritic cells was not up regulated after castration and freshly isolated rhFL induced prostate cells were unable to prime allogeneicT cells unless they were activated by culture either on plastic or with recombinant soluble CD 40 ligand . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) genes and genetic susceptibility to coeliac disease . ^^^ The role of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in T cell activation makes them good candidates for coeliac disease susceptibility genes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of MHC class 2 molecules , CD 40 , and CD 54 was reduced by 40 to 50 % on cells in the lytic cycle , while no decrease was observed in cell surface expression of CD 19 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both native PT ( nPT ) and genetically detoxified PT ( dPT ) efficiently promoted expression on DCs of CD 80 , CD 86 , human leukocyte antigen DR , and CD 83 markers , alloreactive antigen presentation , and cytokine production , primarily interferon ( IFN ) gamma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , stromal fibroblasts as well as monocytes provide a costimulatory signal to anti CD 3 treated T cells via CD 80 and CD 86 to maintain the enhanced activin A production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differentiation of monocytes under different stimulating conditions is also accompanied by the up regulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , adhesion molecules ( CD 54 ) , human leukocyte antigen ( HLA ) DR , synthesis of cytokines tumor necrosis factor alpha , IL 12p70 , IL 10 , and differential expression of message for Toll like receptors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC cultured by both methods expressed high levels of CD 86 , CD 80 , CD 40 , CD 83 , CD 44 , CD11c and CD 58 , and was comparable in their ability to induce allogeneic and peptide specific autologous proliferative responses as well as gp 100 peptide specific cytotoxic T cell responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The ratio of CD 80 : CD 86 expression was significantly lower on CD 4 ( + ) and F4 / 80 ( + ) spleen cells in CD 28 peptide treated mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
More recently , the additional expression of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on antigen presenting cells were found to play an important role in the activation of T lymphocytes in transplant rejection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upon ligation by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) , CD 28 induces T cell proliferation , cytokine production , and effector functions , whereas CTLA 4 signaling inhibits expansion of activated T cells and induces tolerance . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : PTL inhibited the upregulation of CD 80 , CD 83 , CD 86 , CD 40 , and MHC class 2 ; the allostimulatory function ; the production of TNF alpha and IL 12 ; and the downregulation of FITC labeled dextran uptake in human monocyte derived DCs stimulated with LPS but not with TNF alpha . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
FACS analysis of CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , and HLA DR expression on MDDC could not demonstrate significant differences in the expression of these molecules between type 1 and type 2 diabetes patients and healthy controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Surface marker expression ( HLA DR , CD 54 , CD 80 , and CD 86 ) on these DC was measured by flow cytometry after 48 h treatment with the known chemical allergens dinitrofluorobenzene ( DNFB ) and methylchloroisothiazolinone / methylisothiazolinone ( MCI / MI ) , the irritant sodium dodecyl sulfate , lipopolysaccharide ( LPS ) , and TNFalpha . ^^^ For KG 1 DC , only MCI / MI upregulated CD 86 expression , whereas TNFalpha upregulated CD 54 and slightly upregulated CD 80 and CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 2 IgG2b but not IL 15 IgG2b fusion protein reduced migration of antigen presenting cells from the skin to local lymph nodes , inhibited the expression of CD 80 and CD 86 , and induced a significant higher number of CD4+CD25+ T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In comparison with fresh DCs , the thawed DCs had similar morphology , purity , and expression of class 1 ( H 2D ( b ) and H 2K ( b ) ) and class 2 major histocompatibility complex ( MHC ) proteins , as well as CD11b , CD11c , CD 40 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC ( RMAT ) displayed an up regulated expression of immune molecules ( Ia ( d ) , CD 40 , CD 54 , CD 80 and CD 86 ) and certain cytokines / chemokines , and enhanced ability of allogeneic T cell stimulation when compared to DC ( IMAT ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , CD 80 was detected on 2 6 % of freshly isolated pro and pre B cells , and IL 7 stimulation led to induction of CD 86 on 15 20 % of pro and pre B cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Maturation of dendritic cells was induced by stimulation with native HSP 70 , was not seen with the N terminal HSP 70 ( 1 358 ) , but was enhanced with HSP 70 ( 359 610 ) , as demonstrated by up regulation of CD 83 , CCR 7 , CD 86 , CD 80 , and HLA class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment with CD 80 and CD 86 antibodies prior to administration of HgCl 2 completely suppresses the autoimmune process . ^^^ CD 80 and CD 86 antibodies , or an isotype control , were given daily for 3 days and then on alternate days until day 12 commencing either on the day of the first HgCl 2 injection ( day 0 ) or on days 4 or 8 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cross linking of MHC class 2 molecules on human monocyte derived DC results in the up regulation of the surface expression of CD 83 , CD 80 , CD 86 , CD 54 , CD1a and CD 40 molecules , the typical DC maturation associated markers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The transition from monocyte to DC is characterized by the up regulation of CD 83 , CD 86 , CD 80 , CD 40 and the down regulation of CD 14 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
With newly generated ICOS ligand ( ICOS L ) specific monoclonal antibodies we determined that human Langerhans cells in situ express similar levels of ICOS L , CD 80 , and CD 86 , compared to immature dendritic cells ( DC ) derived from monocytes in vitro . ^^^ Maturation of DC strongly up regulated CD 80 and CD 86 but did not significantly change ICOS L levels . ^^^ Our data thus indicate a selective regulation of IL 10 secretion by ICOS on re activation of effector T cells with professional antigen presenting cells ( bearing CD 80 and CD 86 ) in lymphoid tissue . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : In summary , no difference was found between both immature as well as mature DCs generated from patients and controls regarding the expression of CD1a , CD 80 , CD 86 , MHC class 1 , and MCH class 2 antigens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Differential effects of iron deficiency on the expression of CD 80 and CD 86 co stimulatory receptors in mitogen treated and untreated murine spleen cells . ^^^ The interaction of CD 28 and its ligands ( CD 80 , CD 86 ) on antigen presenting cells and that of TCR / CD3 MHC are required for T lymphocyte activation . ^^^ To determine whether impaired lymphocyte proliferation associated with iron deficiency is due to reduced expression of these ligands , spleen cells obtained from eight to nine C57BL / 6 mice / group of iron deficient ( ID ) , iron replete ( R ) , control ( C ) , pair fed ( PF ) , and high iron ( HI ) mice were labeled with anti CD 80 fluorescein isothiocyante ( FITC ) and anti CD 86 FITC . ^^^ Lymphocyte proliferative responses to mitogens positively and significantly correlated with CD 80 and CD 86 FI ( r = 0 . 41 0 . 59 ) but negatively correlated with the percentages of CD 80 + cells ( r = 0 . 48 ) ( P < 0 . 05 ) . ^^^ Data suggest that impaired lymphocyte proliferation associated with iron deficiency is not due to reduced CD 80 and CD 86 expression . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The absolute number and the expression of MHC 1 and 2 , CD 80 , and CD 86 both on immature and mature APCs were not significantly different in young and old mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Glomerular endothelium exhibits enhanced expression of costimulatory adhesion molecules , CD 80 and CD 86 , by warm ischemia / reperfusion injury in rats . ^^^ We have demonstrated that the hepatic sinusoidal endothelial cells have potential ability as APCs by expressing the costimulatory adhesion molecule proteins , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , of which expression was enhanced by warm ischemia / reperfusion of the rat liver . ^^^ In this study , we assessed the localization of CD 80 , CD 86 , and intercellular adhesion molecule 1 in the rat kidneys and the influence of warm ischemia / reperfusion with or without a hypercreatinemic condition on the expression of these adhesion molecules in the renal tissues . ^^^ We found that CD 80 , CD 86 , and intercellular adhesion molecule 1 proteins localized on the glomerular and peritubular endothelium and were up regulated after ischemia / reperfusion . ^^^ The relative mRNA levels analyzed by real time reverse transcription polymerase chain reaction showed that CD 80 and CD 86 expressions were constitutively observed and significantly increased for 14 days after the warm ischemia reperfusion with a peak level at Day 3 ( 6 . 7 and 20 . 8 fold increase for CD 80 and CD 86 , respectively ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To this purpose , we assessed 1 ) the expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules on peripheral , splenic , and inflammatory murine Gr 1+ PMN ; 2 ) its modulation upon interaction with C . albicans in vitro , in vivo , and in human PMN ; 3 ) the effect of Candida exposure on the ability of murine PMN to affect CD4+ Th 1 cell proliferation and cytokine production ; and 4 ) the mechanism responsible for this effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mature DC expressed significant amounts of mature DC markers ( CD 83 ) and the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypic analyses revealed that Uro Vaxom activated moDCs displayed a phenotype of mature DCs with high levels of MHC molecules and increased levels of co stimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 are opposing regulators of T cell activation , triggered by the two ligands CD 80 and CD 86 . ^^^ Using CD 80 and CD 86 molecules expressed on transfected cells , we have identified a major difference between these ligands in that CD 80 transfectants have the ability to inhibit activation of resting human peripheral blood T cells via interaction with CTLA 4 , whereas CD 86 transfectants do not . ^^^ Overall , these data provide evidence that CD 80 and CD 86 differ in their interactions with CTLA 4 and that CD 80 appears to be the preferential inhibitory ligand for CTLA 4 working via a population of CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) regulatory T cells . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Under these culture conditions we noticed upregulation or de novo expression of costimulatory molecules CD 40 , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) in 22 / 22 , 15 / 23 and 21 / 23 cases , respectively . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although new molecules continue to be discovered , the functions of B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , CD 28 , cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , inducible costimulator ( ICOS ) , programmed death 1 ( PD 1 ) , OX 40 ( CD 134 ) and CD 40 ligand ( CD40L , CD 154 ) are now sufficiently understood that immunologists are targeting them to manipulate T cells to slow the progression of autoimmune diseases or treat tumours through the increase in T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Adenoviral transduction of monocyte derived DCs ( MO DCs ) resulted in upregulation of CD 80 , CD 86 , and CD 83 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Expression of HLA DR antigens , CD 80 , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule ( VCAM ) , and E selectin was immunohistochemically detected on duct cells from all patients ; however , the expression of CD 86 was limited to only some patients . ^^^ Concomitant expression of CD 80 on duct cells and Th 1 cytokine mRNA , and CD 86 on duct cells and Th 2 cytokine mRNA , was observed . ^^^ Interferon gamma ( IFN gamma ) induced the cultured salivary gland epithelial cells to express HLA class 1 antigens , HLA DR antigens , CD 80 , and ICAM 1 , while tumor necrosis factor alpha ( TNF alpha ) induced the expression of HLA class 1 antigens , CD 80 , CD 86 , and VCAM . ^^^ CD 80 and CD 86 expression of these cells was also suggested to be involved in the activation of Th 1 and Th 2 , respectively . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
HHV 6 infection of monocyte derived immature DCs appeared to induce the up regulation of CD 80 , CD 83 , CD 86 , and HLA class 1 and class 2 molecules , suggesting that HHV 6 infection induces the maturation of DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we have re examined the specificity of CTLA 4 inhibition of MAPKs by using natural ligand with ex vivo purified CD 4 ( + ) T cells deficient in CD 80 and CD 86 ( double knockout ) , or CTLA 4 , CD 80 , and CD 86 ( triple knockout ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In correlation with their selective effects on humoral and cellular immune responses , these chemokines also differentially attract CD 4 ( + ) versus CD 8 ( + ) T cells and modulate CD 40 , CD 80 , and CD 86 expressed by B 220 ( + ) cells as well as CD 28 , 4 1BB , and gp 39 expression by CD 4 ( + ) and CD 8 ( + ) T cells in a dose dependent fashion . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression in human tubular epithelial cells in vivo and in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Allostimulatory capacity in mixed leukocyte reaction and DC marker expression ( CDla , CD 80 , CD 86 ) was not different between control and FcgammaRI cross linked DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Antigen receptor triggered upregulation of CD 86 and CD 80 in human B cells : augmenting role of the CD21 / CD19 co stimulatory complex and IL 4 . ^^^ In the absence of IL 4 , BCR : CD 21 co ligation augmented BCR triggered CD 86 only under conditions of very low BCR ligand dose or affinity , and CD 80 was minimally induced by BCR and / or CD 21 crosslinking . ^^^ In the presence of IL 4 , BCR : CD 21 co ligation augmented CD 86 and CD 80 expression under conditions of greater BCR engagement . ^^^ Co ligation promoted CD 86 and CD 80 expression was associated with heightened B cell activation of resting allogeneic T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , up regulation of MHC class 2 , CD 80 and CD 86 was not altered . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here we obtained the first evidence that DC , in the absence of any foreign antigens , induce the expression of B 7 family costimulatory molecules , such as CD 80 , CD 86 , B 7 H1 , PD L 2 , B 7 H3 , and B7RP 1 , on autologous T lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OBJECTIVE : To study the modulation of the expressions of human leucocyte antigen ( HLA ) and co stimulatory molecules B 7 ( CD 80 , CD 86 ) in multidrug resistant ( MDR ) breast cancer cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we examined the contribution of the major costimulatory molecules , CD 40 ligand ( CD40L ) , CD 80 , and CD 86 , in the priming of CD 8 ( + ) T cells . ^^^ However , costimulation via either CD 80 or CD 86 was required , as therapeutic T cells could be generated from mice injected with either anti CD 80 or anti CD 86 Ab alone , but administration of both Abs completely inhibited the priming of therapeutic T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , other molecular components including invariant chain ( 2 ) , HLA DM , and costimulatory molecules CD 80 , CD 86 and CD 40 , are required for efficient T cell activation . ^^^ Surface expression of CD 80 , CD 86 or CD 40 was not detected although mRNA for these costimulatory molecules was present in all samples . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , when stressed apoptotic 12B1 D 1 cells were coincubated with immature dendritic cells for 24 hours , this resulted in greater up regulation of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) on the surface of dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Transfection of the macrophagelike cell line THP 1 with the GILZ gene inhibited the expression of CD 80 and CD 86 and the production of the proinflammatory chemokines regulated on activation normal T cell expressed and secreted ( CCL 5 ) and macrophage inflammatory protein 1alpha ( CCL 3 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We also examined the influence of RXM on co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expressions on cultured splenic B lymphocytes induced by in vitro antigenic stimulation using flow cytometry . ^^^ Addition of RXM at a concentration of 5 . 0 microg / ml into cell cultures also suppressed co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expressions on splenic B lymphocytes , which was enhanced by antigenic stimulation in vitro . ^^^ Oral RXM administration for 4 weeks clearly suppressed the enhancement of CD 40 and CD 86 ( but not CD 80 ) expressions on splenic B lymphocytes induced by antigenic stimulation in vivo . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The resulting DC population exhibited typical DC morphology and expressed higher levels of MHC class 2 and the co stimulatory molecules CD 80 and CD 86 compared to the untreated peritoneal cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
No significant changes could be found in the cell surface expression of CD 80 , CD 86 , MHC 1 , MHC 2 , CD 18 , CD11b , CD 40 , CD 25 , and CD 54 in antigen presenting cells ( APC ) from control or DSG treated animals . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of surface molecules , including major histocompatibility complex ( MHC ) classes 1 and 2 , as well as CD 80 and CD 86 , increased on DCs that were stimulated with lipopolysaccaride ( DCs / LPS ) , in comparison with unstimulated bone marrow derived DCs ( BM DCs ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The fact that CD 83 is strongly upregulated together with co stimulatory molecules such as CD 80 and CD 86 during DC maturation suggests it plays an important role in the induction of immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In about 7 days , irregularly shaped floating cells with protrusions appeared and these expressed major histocompatibility complex class 2 , CD11c , CD 80 , and CD 86 , with the capacity to stimulate primary mixed lymphocyte reaction ( MLR ) and to process and present protein antigen to T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 molecule expression showed no difference in MDA MB 435 or HBL 100 cell ( P > 0 . 05 ) , and those of the other four breast cancer cells were lower than that of HBL 100 ( P < 0 . 05 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : We found that : 1 ) mDC generated in the presence of GST showed lower expression of CD1a , CD 83 , CD 80 , CD 86 , HLA ABC and HLA DR compared to control mDC on FACS analysis . 2 ) GST treated mDC showed reduced capacity of stimulating allogenic T cells in mixed leukocyte reaction . 3 ) IL 12p70 production after stimulation with SAC or LPS plus IFN gamma was markedly reduced in GST treated mDC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After phagocytosis , cells were stained for MHC class 2 molecules , CD 14 , CD 80 , and CD 86 to identify the phagocytic population . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These mature DCs could be MHC class 2 negative , but had to bear CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Role of CD 80 , CD 86 , and CTLA 4 on mouse CD 4 ( + ) T lymphocytes in enhancing cell cycle progression and survival after activation with PMA and ionomycin . ^^^ Cell surface interactions between the T cell costimulatory receptors , CD 28 and cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , with their cognate ligands , CD 80 and CD 86 , on antigen presenting cells play an important role in T cell activation . ^^^ Although CD 80 and CD 86 are induced on T cells after activation , not much is known about their role in modulating T cell function . ^^^ We show that CD 80 , CD 86 , and CTLA 4 are induced on purified CD 4 ( + ) T cells after in vitro activation with phorbol 12 myristate 13 acetate ( PMA ) and ionomycin , and they play an essential role for proliferation and survival . ^^^ This study reveals a functional role for CD 80 , CD 86 , and CTLA 4 on CD 4 ( + ) T lymphocytes and sheds light on the mechanisms by which these molecules enhance activation and survival with PMA and ionomycin . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , the required CD154 / CD40 interaction is shown to be independent of CD 80 and CD 86 expression : CD80 / CD86 ( / ) B 6 mice develop MAIDS after LP BM 5 infection . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells were CD 10 , CD 44 , CD 54 , CD 80 , CD 86 and HLA DR positive , as previously described . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In general , DC phenotype was CD 14 ( low ) , CD 86 ( high ) , CD 40 ( high ) , CD 80 ( low ) , and CD 83 ( low ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Chronic stimulation with adenosine enhanced the macropinocytotic activity and the membrane expression of CD 80 , CD 86 , major histocompatibility complex ( MHC ) class 1 , and HLA DR molecules on iDCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cells phenotypes were identified using three color flow cytometry analysis for the following receptors HLA DR , CD 80 , CD 83 , CD 86 , CD11c , CD 18 , CD 54 , CD 58 , CD 3 , CD 4 , CD 8 , CD 19 , CD 20 , CD 14 , CD 16 , CD 56 . ^^^ HLA DR molecules , co stimulatory receptors CD 80 , CD 86 , CD 83 and adhesion molecules CD 18 , CD11c , CD 54 , CD 58 , were analyzed by two color immunofluorescence microscopy on ST serial sections . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Atorvastatin suppressed IFN gamma inducible expression of CD 40 , CD 80 and CD 86 co stimulatory molecules . l Mevalonate , the product of HMG CoA reductase , reversed atorvastatin ' s effects on antigen presenting cells ( APC ) and T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , after exposure to BCG infected necrotic macrophages , DCs underwent phenotypic changes , including the up regulation of maturation specific markers ( major histocompatibility complex class 2 , CD 40 , CD 80 , and CD 86 ) and the capacity to stimulate antigen specific CD4+ T cells with higher efficiency than when they were directly infected with a similar number of bacteria . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They expressed very low levels of costimulatory molecules ( intercellular adhesion molecule [ ICAM ] 1 , CD 40 , CD 80 , CD 86 ) and of the T cell area homing chemokine receptor CCR 7 , whereas they showed higher levels of CCR 2 and CCR 6 , as revealed by reverse transcription PCR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The contribution of IL 16 to the activity was evaluated through use of a neutralizing monoclonal antibody ; the contribution of CD28 / B7 costimulation to allergen induced release of TLCA was determined through use of CTLA 4 Ig fusion protein and neutralizing monoclonal antibodies to CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) . ^^^ Antibodies to the CD 28 counter ligands CD 80 and CD 86 inhibited allergen induced release of TLCA in mild asthmatic explants by 94 % ( P < . 05 ) and 62 % , but TLCA release from moderate asthmatic explants was unaffected by CTLA 4 Ig . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We investigated if B 7 H1 would be up regulated in HIV infection , a disease characterized by increased IL 10 production , by measuring B 7 H1 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) expression and mRNA in 36 HIV infected patients and in 22 healthy controls ( HCs ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Combination induction therapy with monoclonal antibodies specific for CD 80 , CD 86 , and CD 154 in nonhuman primate renal transplantation . ^^^ BACKGROUND : Antibodies and fusion proteins specific for CD 80 , CD 86 , and CD 154 have shown promise as agents capable of inducing donor specific tolerance in rodents . ^^^ In the nonhuman primate , monoclonal antibodies specific for CD 80 and CD 86 have prolonged the time to rejection of renal allografts but have not resulted in tolerance . ^^^ METHODS : A combination of monoclonal antibodies specific for CD 80 , CD 86 , and CD 154 were used in a mismatched nonhuman primate renal allograft model . ^^^ RESULTS : Treatment of four rhesus macaques with this combination yielded a mean rejection free survival of 565 days ( 311 911 days ) , significantly greater than untreated controls ( mean survival=7 . 0 days , P=0 . 001 ) and animals treated with only a combination of anti CD 80 and CD 86 ( mean survival=191 days , P=0 . 01 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upon cultivation with interferon gamma ( IFN gamma ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) polymorphonuclear neutrophils ( PMN ) acquire characteristics of dendritic cells , including expression of major histocompatibility complex ( MHC ) class 2 antigens , of the co stimulatory antigens CD 80 , CD 86 and of CD 83 , the latter considered to be specific for dendritic cells . ^^^ During acute infection up to 80 % of PMN acquired CD 83 , but remained negative for MHC class 2 , CD 80 or CD 86 . ^^^ PMN of patients with primary vasculitis expressed MHC class 2 antigens , CD 80 and CD 86 , but not CD 83 , indicating that up regulation of MHC class 2 and of CD 83 are not necessarily linked to each other . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 4 T cell activation is positively ( CD 28 ) and negatively ( CTLA 4 ) regulated by the costimulatory ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CD 86 ( + ) major histocompatibility complex class 2 ( + ) ( MHC 2 ) ( + ) cells and CD 28 ( + ) CD 4 ( + ) T cells were decreased by SST treatment , while CD 80 ( + ) MHC 2 ( + ) cells , CD 40 ( + ) MHC 2 ( + ) cells and CD 154 ( + ) CD 4 ( + ) T cells showed no change . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD11c+ cells from ProGP 4 treated mice co expressed DEC 205 and also expressed CD 80 , CD 86 and CD 40 , albeit at lower levels as compared to Class II+ / CD11c+ cells from untreated animals . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To determine whether this effect might involve modulation of the expression of accessory molecules known to be important in T cell activation , we analyzed by flow cytometry the influence of vIL 10 on the basal as well as on IFN gamma induced up regulation of HLA molecules , ICAM 1 , and two members of the B 7 family B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) at the surface of human monocytes . ^^^ Viral IL 10 in a concentration dependent manner inhibited both basal and IFN gamma induced HLA class 2 , ICAM 1 ( basal levels of ICAM 2 and ICAM 3 is unaffected ) , CD 80 , and CD 86 up regulation when added simultaneously with IFN gamma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulation of CD11c ( + ) B 220 ( + ) pDCs with oligodeoxynucleotides containing certain CpG motifs plus CD 40 ligand plus GM CSF led to increased MHC class 2 , CD 80 , CD 86 , and CD8alpha expression levels , to a switch in chemokine receptor expression that affected their migration , to IFN alpha and IL 12 secretion , and to the acquisition of priming capacities for both CD 4 ( + ) and CD 8 ( + ) OVA specific TCR transgenic naive T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These included T cell costimulatory molecules CD 80 , CD 86 , CD 40 , and CD 54 and high levels of surface MHC class 1 and 2 on CD11c ( + ) cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC maturation was monitored by flow cytometric analyses for the expression of surface markers ( HLA DR , CD1a , CD 40 , CD 80 , CD 86 , CD 83 , CD 14 , CD 32 , mannose receptor , and CD11c ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
High expression of CD 28 , CD 80 , CD 86 , CD 40 , CTLA 4 , CD44v variants , and FasL occurred in alopecia areata resistant mouse spleens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 80 , CD 83 , CD 86 , and major histocompatibility complex class 1 and class 2 antigens were reduced on lipopolysaccharide ( LPS ) matured leukaemic DC but were enhanced by a mixture of interleukin 1beta ( IL 1beta ) , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and prostaglandin E 2 ( PGE 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Forty to fifty percent of both samples , frozen / thawed as well as fresh BMDC , exhibited characteristic DC morphology , and the DC obtained from the frozen / thawed samples expressed a similar level of MHC class 1 , MHC class 2 , CD 80 , CD 86 , CD11c , CD11b , CD 54 and CD 205 molecule as fresh DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
To observe the proliferation of T lymphocytes stimulated by CML and AML cells which were induced by rhGM CSF and rhIL 4 , and the secretion of IFN gamma from proliferated T lymphocytes , the expression of CD 80 , CD 86 and HLA DR on CML and AML cells induced by GM CSF and IL 4 was assayed by flow cytometry in vitro . ^^^ The results showed that GM CSF and IL 4 significantly upregulated the expression of CD 80 , CD 86 and HLA DR on CML cells and CD 80 and CD 86 on AML cells , which could stimulate the T lymphocyte proliferation and high secretion of IFN gamma ( in CML group ) of autologous T lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The phenotype of DC was detected by FCM with CD1a , CD 40 , CD 80 , CD 86 , HLA A , B , C and HLA DR monoclonal antibodies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Therefore , the authors analyzed the expression kinetics of MHC class 2 ( 1 Ak ) / HEL peptide `` complex , ' ' total MHC class 2 , CD 80 , and CD 86 on in vitro or in vivo CD 40 stimulated DCs over a period of 5 days . ^^^ When purified DCs were co cultured in vitro with antigen specific T cell hybridomas , the DCs had lower expression of total MHC class 2 and `` complex , ' ' but did not reduce their CD 80 and CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD8alpha ( + ) DC from schistosome infected mice exhibited a 2 to 3 fold increase in the expression of MHC class 2 , CD 80 , and CD 40 ( but not CD 86 ) compared with DC from uninfected control animals , while CD8alpha ( ) DC exhibited a 2 to 3 fold increase in the expression of MHC class 2 and CD 80 and no alteration , compared with DC from uninfected mice , in the expression of CD 86 or CD 40 . ^^^ In contrast , infection with T . gondii resulted in a more pronounced increase in the expression of activation associated molecules ( MHC class 2 , CD 80 , CD 86 , and CD 40 ) on both CD8alpha ( ) and CD8alpha ( + ) splenic DC and promoted elevated IL 12 production by DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC infected with S . typhimurium up regulated cell surface expression of major histocompatibility class 1 ( MHC 1 ) , MHC 2 , CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Preadipocytes rapidly and massively acquired high phagocytic activity and index . 60 70 % of preadipocytes also expressed five macrophage specific antigens : F4 / 80 , Mac 1 , CD 80 , CD 86 , and CD 45 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendritic cells ( DCs ) not only induce but also modulate T cell activation . 1 , 25 dihydroxyvitamin D ( 3 ) [ 1 , 25 ( OH ) ( 2 ) D ( 3 ) ] induces DCs with a tolerogenic phenotype , characterized by decreased expression of CD 40 , CD 80 , and CD 86 costimulatory molecules , low IL 12 and enhanced IL 10 secretion . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Large cells , differentiated after seven days of culture , expressed high levels of MHC 2 molecules and Mannose Receptor , variable levels of CD 80 and CD 86 , and low levels of CD1a . ^^^ CD40L matured DC from JMML was associated with relevant increase of CD 80 , CD 86 and CD 83 , increased APC activity , responded in chemotaxis assays to MIP 3beta and secreted increased amounts of macrophage derived chemokine ( MDC ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Uptake of antigen by DC and the degree of expression of the cell surface markers MHC class 2 , CD 80 , CD 86 and the DC maturation marker CD 83 , was investigated by flow cytometry following incubation with liposomes or solution containing FITC conjugated antigen . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upon exposure to either extracellular or intracellular bacteria , DC underwent maturation as assessed by the increased levels of expression of CD 80 , CD 86 , and HLA molecules and the de novo expression of CD 83 and CCR 7 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Like the positive control TNF alpha , the mistletoe extract significantly activated CD 80 and CD 86 as well as HLA class 1 and 2 molecules on these cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that , unlike H2O2 , reactive oxygen species generated by the reaction of oxidase on xanthine induced early phenotypic maturation of dendritic cells by upregulating specific markers CD 80 , CD 83 , and CD 86 and downregulating mannose receptor mediated endocytosis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : MHC class 2 ( + ) CD 80 ( + ) CD 86 ( + ) cells were found in the periphery of the anterior normal stroma . ^^^ In a surprising finding , significant numbers of MHC class 2 ( ) CD 80 ( ) CD 86 ( ) cells were found in the center of the anterior stroma . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 40 ligation had no effect on cell viability or surface expression of CD 54 , CD 80 , CD 86 or CD 95 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both Ad Neo and Ad IL 4 transduced DC displayed upregulated surface MHC class 2 and costimulatory molecules ( CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC infected with any of the Ad vectors expressed higher levels of CD 40 , CD 80 , and CD 86 molecules than uninfected DC and Ad . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , with Der p 1 pulsed P DC from both groups , no increase in human leukocyte antigen DR , CD 80 , and CD 86 and no IL 10 secretion were detected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cultured DCs were shown to express class 1 , class 2 , CD 80 and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The most mature APC were generated by calcium ionophore A 23187 plus IL 4 , as evidenced by the higher expression of CD 40 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC differentiated in the presence of rapamycin ( rapa DC ) had increased expression of CD1a , CD1b , and CD1c and decreased expression of MHC 1 , MHC 2 , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upon stimulation by infectious agent products , dendritic cells ( DC ) become activated , express high levels of class 1 and class 2 antigens , CD 80 , CD 86 and CD 83 and migrate to secondary lymphoid organs where they can prime naive CD 4 helper and CD 8 cytotoxic T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After being stimulated with PTX , DC exhibited up regulation of MHC class 2 , CD 80 , CD 86 , CD 40 , and DEC 205 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 C domains play an important role in receptor binding and co stimulatory properties . ^^^ CD 80 and CD 86 expressed on the surface of antigen presenting cells interact with cytotoxic T lymphocyte antigen 4 [ CTLA 4 ( CD 152 ) ] expressed on activated T cells and mediate critical T cell inhibitory signals . ^^^ CD 80 and CD 86 are type 1 glycoproteins , and are made up of two extracellular ( EC ) Ig like domains a transmembrane region and a cytoplasmic tail . ^^^ Co crystallographic structures of both CD 80 and CD 86 bound to CTLA 4 indicate that there is no direct interaction of the C domain of either CD 80 or CD 86 with the CTLA 4 . ^^^ To further understand the importance of C domains in the functioning of CD 80 and CD 86 , we constructed chimeric human CD 80 and CD 86 molecules by swapping their respective C domains , and tested their ability to stimulate T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Many solid tumours have been shown to lack expression of either of the immune co stimulatory molecules CD 80 ( B7 . 1 ) or CD 86 ( B7 . 2 ) , which is thought to be one of the ways in which tumours may escape immune recognition . ^^^ We have examined the surface expression of CD 80 , CD 86 , human leucocyte antigen ( HLA ) class 1 and 2 , CD11a , CD 54 , and CD 58 on the blast cells from patients with acute myeloid leukaemia ( AML ) at presentation . ^^^ There was no significant difference in duration of first remission with expression of CD 80 , CD 86 , CD11a , CD 54 or HLA class 2 . ^^^ However , when expression of CD 80 and CD 86 were considered together , a significantly longer duration of remission was found . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : The T DCs expressed specific surface markers ( CD 83 , CD1a , CD 54 , CD11a , CD 80 , CD 86 , HLA DR , CD83HLA DR ) of mature DCs , and stimulated strong T cell proliferative responses in both autologous and autogeneic MLR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
MSC express major histocompatibility complex ( MHC ) class 1 and lymphocyte function associated antigen ( LFA ) 3 antigens constitutively and MHC class 2 and intercellular adhesion molecule ( ICAM ) 1 antigens upon gamma interferon treatment but do not express CD 80 , CD 86 , or CD 40 costimulatory molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Such modifications include a reversal of CD4 / CD8 ratio , activated phenotype ( increased expression of CD45RO , CD 95 , and HLA DR ) , and acquisition or increased expression of co stimulatory molecules ( CD 80 , CD 86 , and CD 40 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , cellular activation markers ( CD 69 , CD 25 , HLA DR ) on T lymphocytes and costimulatory molecules ( CD 80 , CD 86 ) on macrophages were studied . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment of microglia with supernatants from allo antigen and myelin basic protein specific Th 1 cell lines augmented expression of cell surface molecules MHC class 2 , CD 80 , CD 86 , CD 40 , and CD 54 , enhanced the functional antigen presenting cell capacity of microglia in a mixed lymphocyte reaction , and increased cytokine / chemokine secretion ( TNFalpha , IL 6 , and CXCL10 / IP 10 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In a parallel paper , we had also shown that oral mucosa biopsies fixed in PLP showed good morphology and immunolabeling of CD 54 , CD 80 , CD 83 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
GM CSF also stimulated differentiation and maturation of TDC as judged by higher expression of MHC class 1 and 2 molecules , CD 54 , CD 80 and CD 86 . ^^^ The influence of unstimulated TDC on proliferation of thymocytes was inhibited by anti CD 86 but not anti CD 80 mAb , whereas in cultures with GM CSF treated TDC both mAbs exerted an additive blocking effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
GM CSF recruited DCs were CD11c ( + ) DEC 205 ( ) and had high expression of major histocompatibility complex ( MHC ) class 2 , CD 54 , and CD 80 but low CD 40 and CD 86 staining . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In support of this hypothesis , recent studies have shown that neutrophils , when activated by the correct combination of cytokines , can be induced to express cell surface major histocompatibility complex ( MHC ) Class 2 ( DR ) antigen , CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) : molecules required for antigen presentation and subsequent T cell activation . ^^^ Cytoplasmic MHC Class 2 antigen was found with two out of 20 normal donors tested whereas cytoplasmic CD 80 and CD 86 were found to a variable extent within all normal donors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , hCD 152 hCD59 or pCD 152 hCD59 expression resulted in a significant reduction in T cell activation as the result of CD 152 engagement of porcine CD 86 or murine CD 80 in when Jurkat cells were cocultured with the hCD 152 hCD59 or pCD 152 hCD59 expressing cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We investigated the pattern of expression of the B 7 family of immunomodulatory molecules B 7 H1 ( PD L 1 ) , B 7 2 ( CD 86 ) , and B 7 1 ( CD 80 ) at the term maternal fetal interface . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After incubation with either NL 4 3 or JR CSF , IPCs produced a large amount of IFN alpha and at the same time underwent morphological differentiation into DCs with upregulation of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 is an important costimulatory molecule for T cells , and it has been shown that cell surface expression of its ligands , CD 80 and CD 86 , can enhance cellular immune responses against tumor cells , however , these tumor cells do not normally express the ligands . ^^^ Many new vaccines will be based upon soluble recombinant antigens , and in vaccination with these antigens CD 80 and CD 86 would normally be expressed on activated antigen presenting cells and additional stimulation through CD 28 would not be predicted to enhance responses further . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We examined the expression in cryostat sections of scalp skin obtained from a total of 28 patients with AA and from five normal control subjects of ( 1 ) molecules related to the induction of cell death including Fas , Fas ligand ( FasL ) , perforin , granzyme B ( GB ) , and TIA 1 , ( 2 ) molecules related to antigen presentation including CD1a , CD 40 , CD 54 , CD 80 , and CD 86 , and ( 3 ) molecules induced by interferon gamma ( IFN gamma ) including CD 40 , CD 54 , Fas , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expanded cells were low in the activation markers CD 25 , CD 69 , CD 71 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We examined their production of IL 1 beta , IL 10 , IL 12p40 , and IL 12p70 , and their expression of CD 23 , CD 40 , CD 54 , CD 80 , CD 83 , CD 86 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Whereas TSN DCs showed a significant reduction in cell surface expression of CD11c , DEC 205 , MHC class 1 antigen , MHC class 2 antigen , CD 80 , and CD 86 as well as a reduction in the transporter associated proteins , transporter associated with antigen processing 1 and 2 , the changes in phenotype and function were not evident when DCs were cultured in supernatant from COX 2 inhibited tumors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of the co stimulatory molecules , CD 54 , CD 80 and CD 86 , remained unaltered on monocytes treated by ECP . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 binds to its ligand ( CD 80 or CD 86 ) and transduces the most important costimulatory signal . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OM 197 upregulated the expression of HLA DR , CD 80 , CD 86 , CD 83 , CD 40 and CD 54 at the surface of myeloid DC naturally present in blood as well as of DC generated in vitro from monocytes using IL 4 and GM CSF . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upregulation of B 7 molecules ( CD 80 and CD 86 ) and exacerbated eosinophilic pulmonary inflammatory response in mice lacking the IFN beta gene . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulation with CD 80 and CD 86 expressed on activated dendritic cells was essential for Th cell escape from suppression at a high antigen dose . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This effect on DC was accompanied by neither an up regulation of costimulatory molecules such as B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , and MHC class 2 molecules nor by an induction of cytokine production , including IL 1 , TNF alpha , IL 10 , and IL 12 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Coexpression of 4 1BBL in these E 7 expressing DCs increased effector and memory CTL responses when they were used for immunization . 4 1BBL expression up regulated CD 80 and CD 86 second signaling molecules in DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We demonstrate that MC exosomes induce immature dendritic cells ( DCs ) to up regulate MHC class 2 , CD 80 , CD 86 , and CD 40 molecules and to acquire potent Ag presenting capacity to T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , CD11c , CD 40 , CD 80 and CD 86 molecules on DCs were not downregulated after treatment with vincristine either in vitro or in vivo . ^^^ In particular , an upregulated expression of CD 40 , CD 80 and CD 86 molecules on DCs was found in the combination treatment group . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The co stimulatory molecules CD 80 and CD 86 give the secondary signal to T lymphocytes that is necessary for the lysis of leukaemia cells , and CD 40 enhances the efficacy of antigen presentation . ^^^ Here , the expression of HLA ABC , HLA A 2 , HLA DR , CD 40 , CD 80 and CD 86 was flow cytometrically examined in blood samples from 24 healthy volunteers ( HV ) , 24 patients with newly diagnosed acute myeloid leukaemia ( AML ) and five patients with relapsed AML . ^^^ The expression of HLA ABC , CD 40 , CD 80 and CD 86 was significantly reduced on LB in comparison with monocytes of HV . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : hMSCs could be subcultured under this culture condition for 20 passages , expressing CD 13 , CD 29 and CD 59 but not CD 11 , CD 14 , CD 31 , CD 34 , CD 45 , CD 80 , CD 86 , CD 117 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The immunopotentiating capacity of CT and CTB linked antigen was associated with both upregulated secretion of interleukin 1beta by the pulsed DC and increased expression of CD 80 and CD 86 on the DC surface . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expected up regulation of expression of the co stimulatory molecules CD 80 and CD 86 was ablated and that of CD 40 was reduced , as were surface CD 83 expression and intracellular CD 208 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These transduced cells retain the ability to upregulate CD 80 and CD 86 similar to control B cell cultures . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both types of DCs expressed similar levels of CD11c , HLA DR , CD 40 , CD 83 , CD 80 and CD 86 before and after lipopolysaccharide stimulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
VZV infection of mature DCs resulted in a selective downregulation of cell surface expression of the functionally important immune molecules major histocompatibility complex ( MHC ) class 1 , CD 80 , CD 83 , and CD 86 but did not alter MHC class 2 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vitro culture of immature DC generated from adherent peripheral blood mononuclear cells ( PBMC ) using granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 4 ( IL 4 ) with OK 432 at various doses ( 0 . 01 to 0 . 1 KE / ml ) for 2 days resulted in increased cell surface expression of CD 80 , CD 83 , CD 86 and ICAM 1 in a dose dependent manner . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The accessory cell function of ALL blasts showed no correlation with the release of immunomodulatory mediators ( IL 2 , IL 10 , IL 15 ) or the expression of any single adhesion / costimulatory membrane molecule ( CD 54 , CD 58 , CD 80 , CD 86 ) by the blasts . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD11c ( + ) ( F4 / 80 ( ) CD 68 ( ) ) dendritic cells ( DC ) in the colonic lamina propria ( cLP ) of normal and immunodeficient ( RAG 1 ( / ) ) C57BL / 6 ( B 6 ) mice show high surface expression of MHC class I / II molecules and CD1d , and low surface expression of CD 40 , CD 80 , CD 86 costimulator molecules . ^^^ Concomitant with colitis development , DC in the inflamed cLP increase in number and up regulate surface expression of CD1d , MHC class 2 molecules and CD 40 , CD 80 , CD 86 costimulator molecules . cLP DC from non transplanted ( healthy ) and transplanted ( diseased ) mice produce similar amounts of IL 12 p 70 and IL 10 in response to CD 40 signaling , but the inducible IL 12 p 40 release is 5 15 fold higher in mice with colitis than in non transplanted mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have examined the role of CD 40 as an independent costimulatory molecule by generating a panel of transfected human fibroblasts expressing DR 1 with either CD 80 , CD 86 or CD 40 . ^^^ On day 3 the dominant costimulation was provided by CD 40 , while by day 5 this was overshadowed by CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We hypothesized that in relapsing remitting MS ( RRMS ) ( with or without of IFNbeta treatment ) and in secondary progressive patients ( SPMS ) IL 12 and costimulatory molecules ( CD 80 [ B 7 1 ] , CD 86 [ B 7 2 ] , CD 28 , CD 40 , CD40L ) would be differentially produced or expressed by MO or T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We showed that BCG could promote cord blood monocyte derived DC maturation by up regulation of CD 80 , CD 83 , CD 86 , CD 40 , and MHC class 2 molecules and down regulation of mannose receptor . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Preferential expression of B7 . 2 ( CD 86 ) , but not B7 . 1 ( CD 80 ) , on B cells induced by CD40 / CD40L interaction is essential for anti DNA autoantibody production in patients with systemic lupus erythematosus . ^^^ RESULTS : Excessive expression of CD 86 and CD 80 molecules was evident on freshly isolated B cells in patients with SLE . ^^^ Anti DNA antibody production by SLE B cells in the presence of activated T cells was markedly inhibited by anti CD 86 , but not anti CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 , CD 86 , CD 28 and Cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) are well known co stimulatory molecules that form the major co stimulatory pathway essential for full activation of T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This process is characterized by increased expression of CD 80 , CD 86 and MHC class 2 and reduced antigen uptake . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expression of these cells was deficient in comparison to adult DC , and functional tests revealed these cells to possess a critical absence of CD 40 signalling . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RMEC also express the T cell costimulatory factor CD 58 but not CD 80 , CD 86 , or CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here we show that CD 2 engagement significantly increases class 2 , costimulatory ( CD 40 , CD 80 , CD 86 ) , adhesion ( CD 54 , CD 58 ) , and CCR 7 molecule expression on primary dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : Antibodies to CD 4 , CD 8 , CD 34 , CD 40 , CD 54 , CD 80 , CD 86 , CD 154 , and HLA DR were used for immunohistochemical analysis of lacrimal gland biopsy specimens obtained from nine patients with cGVHD and five with Sjgren ' s syndrome ( SS ) . ^^^ In addition , CD 4 ( + ) and CD 8 ( + ) T cells were colocalized with mononuclear infiltrates and stromal fibroblasts expressing the full component of surface molecules necessary for antigen presentation , including HLA DR , CD 54 , CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fluorescent antibodies specific for CD11b , CD11c , CD 80 , CD 86 , or major histocompatibility complex ( MHC ) class 2 protein each labeled selective populations of cells in vivo . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BACKGROUND : Macrophage ( MPhi ) receptors of the B 7 family ( CD 80 , CD 86 ) play a crucial role in T cell activation : the lack of costimulation leads to anergy or apoptosis of reactive T cells . ^^^ RESULTS : As compared to PBMPhi , CBMPhi showed a significantly decreased upregulation of CD 80 and / or CD 86 after stimulation with IFN gamma , cAMP , CD40L , and alphaCD 3 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 , CD 86 , and CD 40 expression and IL 12 production were more efficient in AdRGD infected mBM DCs than in conventional Ad infected cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 80 , CD 86 and the macrophage marker CD 68 were determined by immunohistochemistry in biopsies from 40 clinical liver transplants obtained at different time points during the transplantation procedure , and in normal liver tissue obtained from 10 human livers . ^^^ Expression of CD 80 and CD 86 on Kupffer cells was graded by comparison with CD 68 staining . ^^^ In a subgroup CD 80 and CD 86 mRNA was quantified by real time detection polymerase chain reaction . ^^^ CD 80 and CD 86 mRNA and protein expression in liver transplants did not change during the warm ischaemic and reperfusion phases of the transplantation procedure . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , stimulation of human monocytes by MPL led to an up regulation of the costimulatory molecules CD 80 and CD 86 , an effect that could be reduced by pretreatment of cells with a monoclonal antibody to TLR 2 or TLR 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A Mo AM coculture was exposed to 50 micro g / ml of particles and expression of HLA DR , CD 40 , CD 80 , and CD 86 on each cell type was assessed by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , DCs cultured from CML patients who did not achieve a cytogenetic response to IFN alpha expressed significantly lower levels of class 1 HLA , CD 40 , CD 54 , CD 80 and CD 86 than normal DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Unlike dendritic cells , the CD 4 ( + ) CD 3 ( ) cells have little CD 80 or CD 86 expression but do express high levels of the TNF ligands , OX 40 ligand and CD 30 ligand . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules CD 80 and CD 86 in human crescentic glomerulonephritis . ^^^ BACKGROUND : CD 80 and CD 86 , cell surface molecules found only on antigen presenting cells ( APCs ) , are required for activation of CD 4 postitve ( CD4+ ) T cells by interaction with CD28 / cytotoxic T lymphocyte associated antigen 4 on T cells . ^^^ Expression of CD 80 , CD 86 , CD 4 , CD 14 , CD 68 , HLA DR , and intercellular adhesion molecule 1 was investigated in renal tissues using monoclonal antibodies and compared with clinical data at the time of renal biopsy . ^^^ Tubular epithelial cells showed no CD 86 expression , but they expressed CD 80 , and some of them expressed HLA DR . ^^^ CONCLUSION : This study suggests that the costimulatory molecules CD 80 and CD 86 have different expressions in human crescentic GN , and CD 86 is concerned with crescent formation and CD4+ T cell accumulations . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Similarly , B cells showed no change in the expression of class 2 major histocompatibility antigens , LFA 1 , ICAM 1 , CD 19 , CD 40 , CD 80 , CD 86 , CD 95 ( Fas ) , or CD 178 ( FasL ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendritic cells ( DCs ) not only induce but also modulate T cell activation . 1 , 25 Dihydroxyvitamin D ( 3 ) [ 1 , 25 ( OH ) ( 2 ) D ( 3 ) ] induces DCs with a tolerogenic phenotype , characterized by decreased expression of CD 40 , CD 80 , and CD 86 co stimulatory molecules , low IL 12 , and enhanced IL 10 secretion . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulation with flagellin induced increased surface expression of CD 83 , CD 80 , CD 86 , MHC class 2 , and the lymph node homing chemokine receptor CCR 7 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
High levels of Fas ligand and MHC class 2 in the absence of CD 80 or CD 86 expression and a decreased CD4+ T cell Infiltration , enables murine skin tumours to progress . ^^^ Detailed flow cytometric analysis was used to study tumour cell expression of FasL , Fas , CD 80 , CD 86 and MHC class 2 . ^^^ The percentage of progressor tumours expressing MHC 2 was significantly greater than regressor tumours , while neither tumour expressed CD 80 or CD 86 costimulatory molecules . ^^^ The results suggest that progression of skin tumours occurs if tumour cells express high levels of MHC 2 but not costimulatory molecules such as CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fas ligand ( FasL ) , that has the inherent capacities to tether the T cell inhibitor FasL ( CD 95 ligand ) to the surfaces of B 7 ( CD 80 and CD 86 ) positive APC ( via CTLA 4 : B 7 interaction ) , and in so doing , to simultaneously interfere with B 7 to CD 28 T cell activation signals . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dual expression of CD 80 and CD 86 produces a tumor vaccine superior to single expression of either molecule . ^^^ An aggressive subclone of N2a and the less aggressive parental line were transfected with CD 80 , CD 86 , or both molecules and stable lines were established . ^^^ The less aggressive N2a expressing either CD 80 or CD 86 induced anti tumor immunity . ^^^ In contrast , dual expression of CD 80 and CD 86 was required to initiate a protective anti tumor immune response against the aggressive subclone . ^^^ Control of tumor growth was dependent on CD8+ lymphocytes that infiltrated dual expressing ( CD 80 and CD 86 ) lesions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we investigated the role of this costimulatory pathway in resistance to Trypanosoma cruzi infection by using CD 28 deficient mice and blocking antibodies against CD 80 and CD 86 . ^^^ The blockade of both CD 80 and CD 86 by administering specific monoclonal antibodies also exacerbated T . cruzi infection in wild type mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , we have investigated the expression of costimulatory molecules ( CD 40 , CD 80 , CD 86 , PD 1L , B7H2 , OX40L and 4 1BBL ) in human neuroblastoma ( NB ) cells , since virtually no information is available on this issue . ^^^ Primary NB cells showed CD 40 , CD 80 , CD 86 , OX40L , 4 1BBL , but not PD 1L and B7H2 , mRNA expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Attempts at blocking the CD 28 B7s ( CD 80 CD86 ) pathway are currently underway with the receptor fusion protein , LEA29Y a second generation CTL4Aig , and humanized mAbs to CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
MALP 2 and LPS stimulation induced the expression of CD 83 and increased the expressions of CD 80 , CD 86 , HLA ABC and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Tonsil B cells were found to express high amounts of TLR 9 mRNA and protein , and exposure of B cells to CpG ODN but not to an inactive control ODN induced a concentration and time dependent up regulation of the activation markers CD 23 , CD 25 , CD 40 , CD 54 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CX 659S inhibited the expressions of CD 80 and CD 86 , but not that of CD 54 , on Langerhans cells in epidermal cell suspensions . ^^^ Exogenous granulocyte macrophage colony stimulating factor restored the CX 659S induced inhibition of CD 80 and CD 86 expressions of Langerhans cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 4 , CD8alpha , CD 40 , CD 86 , CD 28 , CD 31 and vascular endothelial growth factor was not different between the two tumor groups but expression of CD 40 ligand , CD 80 , NK1 . 1 and CXCR 3 was relatively lower in A11 / Mig tumors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
VEGFR 3 ( + ) DC are CD11c ( + ) CD 45 ( + ) CD11b ( + ) , and are mostly major histocompatibility ( MHC ) class 2 ( ) CD 80 ( ) CD 86 ( ) , indicating immature DC of a monocytic lineage . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) and CD40L molecules by CD 40 positive ( CD40+ ) and CD 4 positive ( CD4+ ) cells in the lymph nodes of sensitized mice were analysed by flow cytometry ( FACS ) . ^^^ FACS analysis of CD40+ cells in the lymph nodes from sensitized mice showed that simultaneous sensitization with MA and LTA did not enhance CD 80 or CD 86 expression by antigen presenting cells such as B lymphocytes and dendritic cells more than sensitization with MA alone . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The data showed that by the addition of STI 571 the dendritic cells derived from CML clone showed an increased expression of CD1a , CD 83 , CD 80 and CD 86 by flow cytometry analysis and showed more intense abilities of allogeneic antigen presentation by mixed leukocyte culture , compared with the control cells without STI 571 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The modulation of immune cell costimulatory molecules , CD 80 , CD 86 , CD 40 , and HLA DR , and the release of the CD 4 ( + ) T cell chemoattractant IL 16 were also investigated . ^^^ The expression of CD 40 , CD 80 , CD 86 , and HLA DR on monocytes , but not on eosinophils , increased in an RSV dose dependent manner . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Productive infection was associated with downmodulation of cell surface CD 83 , CD1a , CD 80 , CD 86 , ICAM 1 , major histocompatibility complex ( MHC ) class 1 , and MHC class 2 on these cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Berberine did not influence the macrophage cell surface expression of the class 2 major histocompatibility complex molecule , the co stimulatory molecules CD 80 and CD 86 , and intracellular adhesion molecule 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
What ' s the difference between CD 80 and CD 86 . ^^^ Intriguingly , they share two ligands , CD 80 and CD 86 , but at present there is no clear model for understanding whether a ligand will promote or inhibit responses . ^^^ Current perceptions are based around the concept that CD 86 is the initial co stimulatory ligand based on its more abundant and earlier expression pattern ; CD 80 has a role following antigen presenting cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This was not due to down regulation of a number of recognized co stimulatory molecules including CD 80 , CD 86 and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Following stimulation via BCR and / or CD 40 surrogate signals for B cells engaged in T dependent signaling differences between the two subsets were seen in the kinetics and / or magnitude of responses such as entry into DNA synthesis , induction of the costimulatory molecules CD 80 and CD 86 ; up regulation of CD 23 , and changes in BCL 6 mRNA expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Reduced CD 80 and CD 86 expression by 1 A ( + ) epidermal cells was also observed . vIL 10 transgenic mice demonstrated a smaller delayed type hypersensitivity response to allogeneic cells upon challenge but had normal contact hypersensitivity to an epicutaneously applied hapten . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Following an additional 3 days of culture in the presence of GM CSF , IL 4 , IL 1beta , IL 6 , TNFalpha , and PGE ( 2 ) , cells ( 82 . 0 + / 5 . 8 % CD83+ ) displayed a mature DC morphology and phenotype , including expression of CD11b , CD11c , CD 18 , CD 25 , CD 40 , CD 54 , CD 58 , CD 80 , CD 86 , HLA class 1 , and HLA DR as well as expression of CCR 7 but not CCR 5 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The enhanced allo stimulatory capacity of splenic DCs from mice injected with 32DTNF alpha cells correlated with increase in the cell surface expression of the costimulatory molecules CD 40 , CD 80 , CD 86 , and major histocompatibility complex ( MHC ) class 2 molecules ( 1 Ak ) , and production of interleukin 12 ( IL 12 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cross species costimulation : relative contributions of CD 80 , CD 86 , and CD 40 . ^^^ Whereas only porcine CD 86 is constitutively expressed on resting endothelial cells , both CD 40 and CD 80 are rapidly expressed after activation . ^^^ METHODS : We have isolated full length cDNA clones for human and porcine CD 80 , CD 86 , and CD 40 . ^^^ Proliferative responses were of comparable magnitude to those obtained when costimulation was provided by human CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment with humanized monoclonal antibodies against CD 80 and CD 86 combined with sirolimus prolongs renal allograft survival in cynomolgus monkeys . ^^^ We investigated the effect of combining humanized anti CD 80 and anti CD 86 monoclonal antibodies ( mAb ) with sirolimus in cynomolgus monkey renal transplant recipients . ^^^ CONCLUSIONS : Sirolimus can be successfully combined with humanized mAb against CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that the 14 day old dendritic cells were CD 80 ( + ) , CD 86 ( + ) , CD 83 ( + ) , CD 54 ( + ) , CD1a ( + ) , CD11b ( + ) , CD11c ( + ) , HLA DR ( + ) , CD 34 ( ) , CD 3 ( ) , CD 19 ( ) , CD 14 ( ) , and CD 16 ( ) . ^^^ Reverse transcription polymerase chain reaction was employed to detect expression of mRNA for CD 80 and CD 86 . ^^^ Differentiating monocytes initially expressed CD 86 while CD 80 appeared on day 2 . ^^^ Differentiating stem cells expressed CD 80 and CD 86 on day 2 of culture . ^^^ The surface expression of CD 80 and CD 86 was studied over the course of differentiation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
By examining surface expression of numerous candidate molecules ( tumor necrosis factor receptor [ TNFR ] 1 , TNFR 2 , CD 36 , CD 40 , CD 47 , CD 80 , CD 86 , PD L 1 , CD 95 ligand , and type 1 interferon receptor ) , we report that IPE cells uniquely express on their surface the costimulatory molecule CD 86 . ^^^ When IPE were blocked with anti CD 86 or were derived from CD80 / CD86 ( but not CD 80 ) knockout ( KO ) mice , the cells displayed reduced capacity to suppress T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The results showed that gamma irradiation reduced expression of CD 86 , CD 80 and HLA DR molecules on dendritic cells , especially CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Specifically , we measured CD 40 , CD 80 and CD 86 expression , lymphoproliferation and interleukin ( IL ) 4 , IL 10 , interferon ( IFN ) gamma and IL 12Rbeta2 expression in the presence and absence of variant cells and their GXMs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using GM CSF , IL 4 , tumor necrotizing factor ( TNF ) alpha , and CD40L , we could induce mature dendritic cells ( DC ) ( positive for CD 45 , CD 80 , CD 83 , and CD 86 ) from the ventricular fluid in all MC patients . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The functions of the rAAV HbsAg infected DCs were assessed by mixed leukocyte reaction ( MLR ) , and the changes of the surface markers ( including CD 80 , CD 83 , CD 86 and HLA DR ) in response to the infection were detected by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , we show that DC are exquisitely sensitive to their local antigenic microenvironment , and that responses generated by one antigen can be altered by another . 10 IL 2 infection of immature DC led to DC activation ( upregulation of CD 80 , CD 86 and class 2 surface molecules ) when the virus was propagated through xenogeneic , but not syngeneic , mammalian cells ; these DC secreted IL 10 and tumor necrosis factor alpha ( TNF alpha ) , but not IL 12 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Effects of immunoregulatory cytokines ( interferon gamma [ IFN gamma ] and interleukin 10 [ IL 10 ] ) and HSV antigens on B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression on monocytes during HSV 2 infection were investigated using flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Changes in the number of CD 80 ( + ) , CD 86 ( + ) , and CD 28 ( + ) peripheral blood lymphocytes have prognostic value in melanoma patients . ^^^ Melanoma patients with thinner tumors and those surviving revealed an increase of CD 19 ( + ) CD 80 ( + ) and CD 19 ( + ) CD 80 ( + ) CD 86 ( + ) cells , as well as a higher concentration of CD 3 ( + ) CD 4 ( + ) CD 28 ( + ) cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We monitored the secretion of tumor necrosis factor alpha ( TNF alpha ) and the surface expression of human leukocyte antigen DR and the costimulatory molecules CD 80 and CD 86 on monocytes / macrophages . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD11c+ DCs from the mediastinal lymph nodes of infected mice selectively stimulated both OVA and influenza specific T cells and underwent maturation , with higher levels of MHC class 2 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , higher expression of interferon gamma ( IFN gamma ) in T cells and higher expression levels of , in order of significance , CD 80 ( 2 . 6 to 3 . 8 fold increase ) , CD 86 , and CD 40 on DCs were also observed . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DCs must express HLA class I / II molecules and the costimulatory molecules CD 40 , CD 80 and CD 86 to effectively activate T cells for the subsequent lysis of leukemic blasts . ^^^ Compared with AML blasts , the expression of CD 40 , CD 80 , CD 86 and HLA DR was upregulated during DC culture to a median of 80 98 % on AML DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendritic cell uptake of human apoptotic and necrotic neutrophils inhibits CD 40 , CD 80 , and CD 86 expression and reduces allogeneic T cell responses : relevance to systemic vasculitis . ^^^ RESULTS : Immature DCs engulfed apoptotic and necrotic neutrophils , resulting in up regulation of CD 83 and class 2 major histocompatibility complex molecules , but down regulation of CD 40 , CD 80 , and CD 86 , and a decreased ability to stimulate T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In inflammation , in addition to a significant increase in the number of DCs , a majority up regulated their expression of MHC class 2 , CD 80 , and CD 86 , indicating their state of maturation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Lovastatin inhibited MHC class 2 and CD 40 expression on DC in a dose dependent manner , but had lesser effects on CD 16 , CD 80 , CD 86 , and CD11b expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We analyzed here IgD ( + ) CD 27 ( + ) B cells ( circulating B cells 2 , cB 2 ) and IgD ( ) CD 27 ( + ) memory B cells ( cB 3 ) in comparison with IgD ( + ) CD 27 ( ) naive B cells ( cB 1 ) . cB 2 were found to be morphologically similar to cB 3 with abundant cytoplasm , whereas cB 3 expressed CD 80 , CD 86 , and CD 95 on their surface more predominantly than cB 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
There were no significant differences in the expression of CD11a , CD 40 , CD 80 and CD 86 or MHC classes 1 and 2 molecules between the diagnostic and relapsed samples . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CT also enhanced the expression of CD 80 , CD 86 , and OX 40 ( CD 134 ) on DC and induced the secretion of the chemokine , macrophage inflammatory protein 2 ( MIP 2 ) , but inhibited LPS driven induction of CD 40 and ICAM 1 expression and production of the inflammatory cytokines / chemokines IL 12 , TNF alpha , MIP 1alpha , MIP 1beta , and monocyte chemoattractant protein 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , VIP / PACAP reduce the expression of costimulatory molecules ( particularly CD 80 and CD 86 ) on the antigen presenting cells , and therefore reduce stimulation of antigen specific CD4+ T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
PL also enhanced the expression of co stimulatory molecules , CD 80 and CD 86 , in murine B cells in a time dependent manner . ^^^ However , PL has no influence on the [ Ca ( 2+ ) ] concentration and the ROI formation in murine B cells , whereas the genistein , protein tyrosine kinase ( PTK ) inhibitor or staurosporine , protein kinase C ( PKC ) , blocked the proliferation and the induction of co stimulatory molecules , CD 80 and CD 86 , in B cells stimulated with PL . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Treatment with DEX did not affect the expression of surface markers , including H 2Kk , 1 Kk , CD 80 and CD 86 , on DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Strikingly , there was also almost complete ( 92 % ) and selective inhibition of the up regulation of the monocyte costimulatory molecule CD 80 , while 1 CAM 1 , LFA 3 , HLA DR , and CD 86 expression were unaffected . ^^^ They had reduced constitutive expression of CD 40 ( 33 % ) and CD 80 ( 61 % ) , but not CD 86 , and marked inhibition of release of IL 6 ( 72 % ) , IL 12 ( 70 % ) , and TNF alpha ( 46 % ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 , but not CD 86 were up regulated on the spleen derived dendritic cells from OVA sensitized and challenged BALB / c mice . ^^^ There is increasing evidence that CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , as the most important costimulatory molecules , are involved in the allergic immune responses . ^^^ In the present study , we investigated the CD 80 and CD 86 expression of spleen derived dendritic cells ( DCs ) in a murine model of allergic asthma . ^^^ In this model , we found that CD 80 were significantly upregulated on the spleen derived DCs from OVA sensitized and challenged mice compared with that from PBS treated or non treated mice , while CD 86 is not different among three groups . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , decidual DCs expressed costimulatory molecules such as CD 80 and CD 86 and a mature marker such as CD 83 on their surface . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The results showed that CD 22 , CD 20 , CD62L , CD 40 , CD 24 , CD79b , CD79a , and FMC 7 were almost positive in the circulating B cell population , whereas CD11a , CD 80 , CD 103 , CD 10 , CD40L , CD 54 , CD95L , CD 86 , and CD 95 were almost negative in the peripheral blood B lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Most importantly , immunophenotypic analyses demonstrated that MPCs did not express major histocompatibility complex class 2 molecules or the T cell costimulatory molecules CD 80 and CD 86 , consistent with further investigation showing that MPCs failed to elicit a proliferative response from allogeneic lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : The long term culture of splenocytes resulted in the generation of a cell line , termed NOD DC 1 , which have a phenotype of myeloid DCs ( CD11c , CD11b , DEC 205 ) , express MHC class 2 and co stimulatory molecules ( CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Maturation markers ( i . e . , CD 80 , CD 86 , major histocompatibility complex class 2 , and CD 40 ) showed enhanced expression on DC incubated with targeted PorA liposomes relative to those incubated with nontargeted PorA liposomes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : The effects on expression of HLA DR and costimulatory molecules ( CD 80 , CD 86 , ICAM 1 , and CD 40 ) on normal conjunctival epithelial cells and ChWK treated with various cytokines ( IFN gamma , TNF alpha , IL 4 , and IL 13 ) were determined using flow cytometry and confocal microscopy . ^^^ For ChWK cells , CD 80 and CD 86 were constitutively expressed at low levels . ^^^ CD 80 was significantly upregulated after IFN gamma treatment ( P = 0 . 043 ) , whereas IL 4 induced a significant upregulation of CD 86 ( P = 0 . 039 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Myelin phagocytosing microglia express MHC 2 and the costimulatory molecule CD 86 , but lack CD 80 , which is found only on activated antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Retrovirus transfected bone marrow cells cultured with GMCSF and IL 4 for 7 days demonstrated 70 80 % of DCs with high CD11c , CD 80 , CD 86 , and MHC class 1 ( 1 Kd ) expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Administration of dendritic cells transduced with antisense oligodeoxyribonucleotides targeting CD 80 or CD 86 prolongs allograft survival . ^^^ We examined the effects of transduction with high affinity antisense oligodeoxyribonucleotides ( ODNs ) designed to target the mRNA of CD 80 or CD 86 on the phenotype and function of dendritic cells ( DCs ) . ^^^ MATERIALS AND METHODS : DCs were propagated from C57BL / 10 ( B 10 ; H2b ) bone marrow cells in granulocyte macrophage colony stimulating factor and interleukin ( IL ) 4 , and transduced with anti CD 80 or anti CD 86 antisense ODNs ( base mismatched ODNs as controls ) . ^^^ Antisense ODNs targeting CD 80 or CD 86 mRNA specifically suppressed the expression of CD 80 or CD 86 in DCs and inhibited their capacity to elicit the proliferative responses , donor specific cytotoxic T lymphocyte activity in C3H ( H2k ) spleen T cells . ^^^ In contrast with the administration of control DCs into C3H recipients that exacerbated rejection of B 10 cardiac allografts , injection of DCs transduced with anti CD 80 or CD 86 antisense ODN significantly prolonged the survival of heart allografts . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These changes correlated with down regulation of co stimulatory and adhesion molecules such as CD 40 , CD 54 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The presence of GSNO during maturation of DCs caused a reduced expression of surface CD 86 , whereas CD 80 , CD 83 , and MHC molecule expression was not affected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Parent OM selectively up regulated Toll like receptor 4 ( TLR 4 ) mRNA expression and induced mo DC maturation , as reflected by increased production of chemokines , proinflammatory cytokines , and CD 83 , CD 80 , CD 86 , CD 40 , and major histocompatibility complex ( MHC ) class 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD 80 or CD 86 costimulatory molecules on tumor cells can produce rejection of immunogenic but not poorly immunogenic tumors . ^^^ Anti CD 3 receptors effectively induced T cell cytotoxicity , whereas coexpression with CD 80 or CD 86 was required for generating T cell proliferation and IL 2 secretion . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We first observed that neonatal pDCs displayed decreased up regulation of CD 80 , CD 83 , CD 86 , and CD 40 , whereas HLA DR and CD 54 up regulation did not differ significantly between adults and neonates . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On the other hand , however , and in contrast to the effects of IL 10 on human DC , we found that the addition of IL 10 from the initiation of the culture onwards induced an up regulation of the expression of the costimulatory molecules CD 40 , CD 80 and CD 86 on murine myeloid DC , as compared to DC generated with GM CSF only . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
TNF alpha markedly increased the expression on DC of major histocompatibility complex ( MHC ) and the costimulatory molecules , CD 40 , CD 80 and CD 86 . ^^^ Both NAC and GSH completely abolished the TNF alpha induced enhancement of CD 40 expression , but had no considerable effect on the expression of CD 80 , CD 86 and MHC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Dendritic cells from healthy ( DC Hs ) or MC 38 tumour bearing mice ( DC TBs ) were examined for endocytotic capacity of FITC labelled dextran , antigen presenting capacity in allogeneic mixed leucocyte reaction ( allo MLR ) and expression of 1 Ab , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The macrophages express elevated levels of cell surface molecules CD 80 , CD 86 , CD 54 and MHC 2 , markers characteristic of antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Transduction had no effect on EBV LCL expression of major histocompatibility complex ( MHC ) antigens or of costimulatory molecules CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
At 12 h postinfection , wt HSV 2 upregulated DC major histocompatibility complex ( MHC ) class 2 expression , and in contrast to UV inactivated HSV 2 , downregulated expression of MHC class 1 , but it had no effect on surface CD 40 , CD 80 , or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The kinetics of CD 80 and CD 86 expression on antigen presenting cells in cedar pollinosis subjects ] . ^^^ CD 80 and CD 86 , which are costimulatory molecules in T cell activation , play important roles in the differentiation of Th 1 or Th 2 phenotypes . ^^^ The results of blocking studies using neutralizing antibodies have suggested that CD 80 and CD 86 also play important roles in sensitization to cedar pollen antigen , but very few studies have examined the kinetics of CD 80 and CD 86 expression on antigen presenting cells ( APC ) . ^^^ We studied the kinetics of CD 80 and CD 86 expression on APC after allergen stimulation in cedar pollinosis subjects . ^^^ Zero to 8 days following in vitro stimulation , the expression of CD 80 and CD 86 in either CD14+ or CD19+ cells was analyzed by two color flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Analysis of intragraft gene and protein expression of the costimulatory molecules , CD 80 , CD 86 and CD 154 , in orthotopic liver transplant recipients . ^^^ Gene and protein expression of CD 80 , CD 86 and CD 154 were analyzed by RT PCR and immunohistochemistry . ^^^ In reperfusion biopsies CD 154 , but not CD 80 or CD 86 , protein expression correlated with the total ischaemic time . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , CCL 16 activated macrophages overexpress membrane CD 80 , CD 86 , and CD 40 costimulatory molecules and extensively phagocytose tumor cell debris . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although IFN DCs had a more mature immunophenotype than IL 4 DCs , showing higher expression of CD 80 , CD 86 , and CD 83 , they still preserved comparable endocytic and phagocytic capacities and responsiveness to maturation stimuli . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both fungal forms induced DC maturation , as indicated by up regulation of CD 83 , CD 80 , CD 86 , CD 40 , and major histocompatibility complex classes 1 and 2 surface antigens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Augmentation of CTL responses correlated with up regulation of CD 80 and CD 86 expression in DCs transduced with costimulatory molecules , suggesting a mechanism for enhanced T cell activation / survival . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The addition of active PSA to DC cultures significantly inhibited the generation and maturation of DC , as assessed by the levels of expression of CD 83 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this work , we examined if resistance of a protein to proteolytic processing affects the expression of costimulatory molecules , CD 80 and CD 86 , on macrophage exposed to the same antigen . ^^^ The murine peritoneal cavity macrophages expressed both CD 80 and CD 86 after 24 h of incubation with HlyA monomer but failed to express the costimulatory molecules when treated with the HlyA oligomer . ^^^ The expression of CD 80 molecule on macrophage after 48 h by the HlyA oligomer that failed to express the costimulatory molecules after 24 h indicates that proteolytic processing plays a decisive role in expression of CD 80 and CD 86 on cell surface . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After culture for 20 hours in vitro , SF neutrophils from RA patients expressed large amounts of surface class 2 MHC but very low levels of costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Effects of CD 80 and CD 86 on cytokine production in patients with wasp venom allergy who receive venom immunotherapy . ^^^ Several studies have provided evidence that activation of antigen specific T cells requires interactions between CD 28 on T cells and its ligands , CD 80 and CD 86 , on antigen presenting cells ( APCs ) . ^^^ However , the effects of CD 80 and CD 86 on cytokine production in patients with Hymenoptera venom allergy who receive venom immunotherapy remain unclear . ^^^ We examined the effects of CD 80 and CD 86 on Th 1 and Th 2 cytokine production before and after venom immunotherapy in patients with wasp venom allergy . ^^^ CD4+ T cells and monocytes were isolated as APCs from PBMCs and were cocultured with wasp venom in the presence of anti CD 80 or CD 86 blocking antibodies . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Supplementation of the formula with TGF beta 2 down regulated the proinflammatory cytokine mRNA as well as the number of activated lymphocytes , eosinophils , mast cells , CD 80 , and CD 86 positive dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Surviving rats were euthanized on day 150 and intragraft gene ( CD 80 , 86 , 152 , 154 , IFN gamma , IL 2 , IL 6 , IL 10 , IL 13 , TNF alpha , TGF beta , IL 7 , Fas ligand , Granzyme B , bax , and bcl ( 2 ) ) and protein ( CD 4 , CD 8 , ED 1 , CD 154 , CD 80 , CD 86 ) expression was measured . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In both the influenza and HIV DNA immunization models , several groups have reported that coimmunization of mice with plasmids encoding immunogen and CD 86 , but not CD 80 , effectively boosts Ag specific T cell activation . ^^^ To examine this issue , we developed a series of chimeric CD 80 and CD 86 constructs as well as deletion mutants , and examined their immune activating potential in the DNA vaccine model . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We performed a phase I / II clinical trial in metastatic melanoma patients with an ultraviolet ( UV ) inactivated nonreplicating recombinant vaccinia virus enabling the expression , from a single construct , of endoplasmic reticulum targeted HLA A 0201 restricted Melan A / MART 1 ( 27 35 ) , gp 100 ( 280 288 ) , and tyrosinase ( 1 9 ) epitopes , together with CD 80 and CD 86 costimulatory proteins . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mice transgenic for the HER 2 / neu homologue , rat neu , were immunized with full length rat neu cDNA given alone or in combination with plasmids encoding costimulatory molecules CD 80 or CD 86 and the ligand for CD 137 ( CD137L ) . ^^^ Immunization with plasmids encoding the neu antigen along with plasmids encoding CD137L and either CD 80 or CD 86 resulted in the generation of neu specific antibodies that induced phopshorylation of the neu tyrosine kinase and inhibited the growth of cultured tumor cells overexpressing neu . ^^^ DNA vaccines encoding neu , when given in combination with both CD137L and either CD 80 or CD 86 , can induce cellular and humoral immunity and result in an antitumor effect . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mature DC showed a high expression of CD 83 , HLA DR , and the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Importantly , Ficoll treated monocytes retained their ability to differentiate to mature DCs demonstrated by morphology , phenotype ( MHC class 2 ( + ) , CD1a ( + ) , CD 80 ( + ) , CD 86 ( + ) , and CD 83 ( + ) ) , ability to stimulate mixed lymphocyte responses ( MLR ) , present antigen , and produce interleukin 12 ( IL 12 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The present study examined the expression of a range of co stimulatory molecules : CD 40 , CD 54 , CD 80 , CD 86 and HLA DR , on the cell surface of CD 14 ( + ) peripheral blood monocytes ( PBM ) from relapsing remitting ( RR ) and secondary progressive ( SP ) MS patients , prior to and during 1 year of Interferon ( IFN ) beta 1a ( Rebif ( R ) ) therapy . ^^^ IFN beta therapy led to a significant reduction in the expression of CD 54 and CD 80 in both groups of patients as well as to elevation of CD 40 and CD 86 expression in SP patients . ^^^ These results confirm IFN mediated modulation of the APC surface within the immunological synapse and implicate CD 80 and CD 86 as targets for interventional therapies in MS as well as other Th 1 mediated autoimmune diseases . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Activation of the innate immune system allows the activation of the adaptive immune response by production of proinflammatory cytokines and by providing stimulatory signals via major histocompatibility complex molecules and costimulatory molecules such as CD 40 , CD 80 , or CD 86 ; together , these lead to the full activation of both immune systems to fight against pathogenic microorganisms . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
One group of mice was vaccinated with an irradiated , 410 . 4 syngeneic mammary tumor cell line co expressing human MUC 1 and CD 80 or CD 86 co stimulatory molecules , and a second group of mice was vaccinated with plasmids encoding MUC 1 and CD 80 or CD 86 . ^^^ There were significant inhibition on rates of tumor growth and survival in mice vaccinated with irradiated 410 . 4 / MUC1 cells co expressing either CD 80 or CD 86 molecules , compared to non vaccinated animals . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast to CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , ICOSL protein and mRNA was expressed in 7 of 12 glioma cell lines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This conclusion was based on the similar phenotype profile of DC , such as the expression of CD1a , CD 80 , CD 86 , and HLA DR , down regulation of CD 14 , and the absence of CD 83 , as well as on their similar allostimulatory activity for T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Function of CD 80 and CD 86 on monocyte and stem cell derived dendritic cells . ^^^ Reverse transcription polymerase chain reaction revealed that differentiating monocytes initially expressed CD 86 mRNA while CD 80 mRNA appeared on Day 2 . ^^^ Differentiating stem cells expressed both CD 80 and CD 86 mRNA on Day 2 of culture . ^^^ Monoclonal antibodies ( mabs ) to CD 80 and CD 86 were employed to assess their costimulatory roles . ^^^ A decrease in stimulation as depicted by decreased T cell activation was significant with mabs to both CD 80 and CD 86 on monocyte derived DCs while only mabs to CD 86 induced decreased T cell activation by stem cell derived DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA4Ig binds to CD 80 and CD 86 on antigen presenting cells , blocking the engagement of CD 28 on T cells and preventing T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Porins and lipopolysaccharide from Salmonella typhimurium regulate the expression of CD 80 and CD 86 molecules on B cells and macrophages but not CD 28 and CD 152 on T cells . ^^^ RESULTS : Our results show that porins of S . typhimurium increase the expression of CD 86 and the expression of CD 80 both on B lymphocytes and macrophages , while the expression of CD 28 and CD 152 on T lymphocytes was unaltered . ^^^ The expression of CD 80 and CD 86 is dose dependent and starts after 24 h post treatment , peaks at 48 h and goes back to the basal value after 72 h . ^^^ CONCLUSIONS : S . typhimurium porins are able to induce a high expression of costimulatory molecules ( CD 80 and CD 86 ) on lymphocytes and macrophages . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We observed that neonatal myeloid DC displayed decreased upregulation of CD 40 , CD 80 whereas CD 86 and HLA DR upregulation did not differ significantly between adults and neonates . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , a downexpression of activation markers ( CD 25 , CD 21 , CD 80 , CD 86 ) on B cells characterized the group MB 1 patients and was associated with an upexpression of activation markers ( HLA DR , CD 95 , CD 57 ) on T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of MHC Class 2 , CD 70 , CD 80 , CD 86 and pro inflammatory cytokines is differentially regulated in oral epithelial cells following bacterial challenge . ^^^ Expression of cell surface molecules ( major histocompatibility complex ( MHC ) Class 2 , CD 54 , CD 70 , CD 80 and CD 86 ) and secretion of inflammatory cytokines ( interleukin ( IL ) 1beta , IL 6 , and tumor necrosis factor ( TNF ) alpha ) in response to selected bacterial challenge were examined on an immortalized oral epithelial cell line , HOK 18A and a skin epithelial cell line , HaCaT . ^^^ Subsequently , cell surface expression of MHC Class 2 , CD 54 , CD 70 , CD 80 and CD 86 , along with pro inflammatory cytokine levels were determined using flow cytometry , ELISA and RT PCR . ^^^ Interestingly , CD 70 , CD 80 , and CD 86 were generally decreased upon bacteria and LPS challenge on HOK 18A . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OK 432 increased expression of activation and maturation related molecules such as HLA DR , CD 80 , CD 83 , and CD 86 in imMo DCs at levels similar to that of TNF alpha plus PGE 2 , and higher than that of LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 86 and CD 80 costimulatory antigens are highly overexpressed on Hodgkin / Reed Sternberg cells in patients with Hodgkin ' s disease ( HD ) and are candidate target antigens for immunotoxins in order to eliminate minimal residual disease . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The generated matured transfected DCs show high expression of the antigens CD 83 , CD 80 , CD 86 and human leucocyte antigen DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
TNF alpha markedly increased the surface expression of major histocompatibility complex ( MHC ) and costimulatory molecules , CD 86 and CD 80 , on DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
They lost the progenitor markers CD 33 , CD 13 and CD 34 while significantly increasing expression of the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fc on the differentiation of DCs was the up regulation of CD 86 and down regulation of CD 80 , suggesting a modulatory potential to skew the T cell response toward the T helper cell type 2 ( Th 2 ) phenotype . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Protection depended on the presence of costimulatory molecules CD 80 , CD 86 , and CD 40 on the DCs , but surprisingly did not require DCs that could make IL 12 or IL 15 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , it profoundly increased CD 80 , CD 86 , MHC 1 , and MHC 2 expression in murine , GM CSF and IL 4 stimulated , BM derived myeloid DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Porin treatment of CD11b+ peritoneal cavity ( PerC ) MPhi of BALB / c mouse was found to up regulate CD 80 on cell surface and had no effect on CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : By flow cytometry , tape stripping augmented the expression of MHC class 2 , CD 54 , CD 80 , CD 86 and CD 40 on Langerhans cells , whereas UVB decreased the expression of some of these molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BOM , NMB and GRP dose dependently inhibited maturation of DC assessed as down regulation of CD 40 , CD 80 and CD 86 expression on DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment of IEC 6 cells with GM CSF enhanced expression of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The cell surface expression of CD 24 , CD 40 , CD 54 , CD 80 , CD 86 , OX40L and MHC class 2 molecules was barely altered following infection with unopsonized promastigotes or amastigotes from nude mice or with C 3 coated promastigotes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , DC derived from acute myeloid leukemia blasts did not show an elevated level of ACE , although they acquired DC markers CD 80 , CD 40 , and CD 86 upon cytokine or calcium ionophore treatment . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 18 strongly up regulated CD 54 on MoDC , whereas the effect on MHC class 2 , CD 83 , and CD 86 was only moderate and the expression of CD 40 and CD 80 was not affected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This is not due to alterations in Ag processing or to an increase in surface expression of CD 80 , CD 86 , ICAM 1 , or MHC 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A lesser expression of costimulatory molecules ( CD 80 and CD 86 ) was seen in DCs treated with exogenous VEGF than in DCs not treated with VEGF . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
EGCG , in a dose dependent manner , profoundly inhibited CD 80 , CD 86 , and MHC class 1 and 2 expression on bone marrow derived murine myeloid DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Herein , we report that Ebola VLPs ( eVLPs ) were immunogenic in vitro as eVLPs matured and activated mouse bone marrow derived dendritic cells , assessed by increases in cell surface markers CD 40 , CD 80 , CD 86 , and MHC class 1 and 2 and secretion of IL 6 , IL 10 , macrophage inflammatory protein ( MIP ) 1alpha , and tumor necrosis factor alpha by the dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Given the critical role of HLA molecules and the costimulatory signals delivered by CD 80 and CD 86 in optimal antigen presentation and T cell activation , inhibition of expression of HLA and CD80 / CD86 on DCs by IVIG offers a plausible explanation for the efficacy of IVIG in SLE and other immune mediated inflammatory conditions . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
HA treatment upregulated the expression of costimulatory molecules such as CD 40 , CD 80 , and CD 86 on DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It expresses MHC 1 and CD95 / Fas , while HLA DR , CD 40 , CD 80 , CD 86 , MUC 1 , MUC 2 , and ICAM 1 / CD54 are absent . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 expression on LPS stimulated monocytes and the effect of CD 80 and CD 86 blockade on IL 4 and IFN gamma production in nonatopic bronchial asthma . ^^^ CD 80 and CD 86 seem to play an important role in the allergen induced secretion of interleukin ( IL ) 5 and IL 13 . ^^^ Up to now , the expressions of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) on monocytes and the kinetics of the expression of these molecules on lipopolysaccharide stimulated monocytes in nonatopic asthma have not been defined . ^^^ Using monoclonal antibodies , we have compared the expressions of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) on the monocytes of healthy persons and nonatopic asthmatic patients . ^^^ We have also assessed the effect of CD 80 and CD 86 inactivation on IL 4 and interferon ( IFN ) gamma production in nonatopic asthmatics and healthy subjects . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The soluble Id CD40L fusion protein retained CD 40 binding activity and stimulated CD 80 and CD 86 upregulation and interleukin ( IL ) 12 production by macrophages . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Presence of spinal B7 . 2 ( CD 86 ) but not B7 . 1 ( CD 80 ) co stimulatory molecules following peripheral nerve injury : role of nondestructive immunity in neuropathic pain . ^^^ Recent studies suggest that co stimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) play a differential role in the effect of beneficial versus deleterious CNS autoimmune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
First , using interleukin 4 ( IL 4 ) / granulocyte macrophage colony stimulating factor ( GM CSF ) induced , blood monocyte derived DC , hapten induced up regulation of maturation / activation markers , including CD 80 , CD 83 , CD 86 , chemokine receptors CXCR 4 and CCR 5 , as well as the drug resistance related molecules P glycoprotein ( Pgp ) and lung resistance protein ( LRP ) , were monitored by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Conversely , the lack of the dominant costimulatory molecules , CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) could be a possible explanation of poor immunogenicity of OVCA tumors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : Airway eosinophils were recovered from ovalbumin sensitized and challenged BALB / c mice , these eosinophils were then cocultured with sensitized CD 4 ( + ) cells in the absence or presence of anti CD 80 or / and CD 86 monoclonal antibodies . ^^^ RESULTS : Our data showed that airway eosinophils , recovered following inhalational ovalbumin challenge in sensitized mice functioned as CD 80 and CD 86 dependent antigen presenting cells to stimulate sensitized CD 4 ( + ) lymphocytes to produce IL 4 , IL 5 and IL 13 , but not interferon gamma ( IFN gamma ) in vitro assay . ^^^ When instilled intratracheally in ovalbumin sensitized recipient mice , these antigen loaded eosinophils migrated into draining paratracheal lymph nodes primed Th 2 cells in vivo for IL 4 , IL 5 and IL 13 , but not IFN gamma , production during the in vitro culture that was also CD 80 and CD 86 dependent . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The role of B 7 ligands ( CD 80 and CD 86 ) in CD 152 mediated allograft tolerance : a crosscheck hypothesis . ^^^ BACKGROUND : The regulatory mechanism by which the B 7 ligands ( CD 80 and CD 86 ) direct the CD28 / CD152 costimulatory pathways is unclear . ^^^ This study investigated the role of CD 80 and CD 86 in a CD 152 mediated allograft tolerance model . ^^^ The influence of anti CD 80 and anti CD 86 treatments on the proliferation and interleukin ( IL ) 2 productions of the tolerated splenocytes ( SC ) was analyzed . ^^^ The role of CD 80 and CD 86 in the induction and maintenance of the graft acceptance in this transplant model were also tested . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Then , the expression of HLA DR , CD 40 , CD 44 , CD 63 , CD 80 , CD 86 , Fas receptor , E cadherin , ICAM 1 , MUC 1 , cytokeratins and vimentin were investigated by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : DCs transfected and matured had increased cell surface expression of CD 40 and costimulatory molecules CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Effects of preactivated autologous T lymphocytes on CD 80 , CD 86 and CD 95 expression by chronic lymphocytic leukemia B cells . ^^^ CLL B cell expression of CD 86 and CD 95 was markedly upregulated using this approach , whereas CD 80 expression was augmented only in a minority of patients ; these effects were partially preserved even when preactivated T cells were rechallenged with CLL B cells at the same low T / B cell ratio as that observed in vivo . ^^^ Finally , CD 80 upregulation on CLL B cells appeared to be mainly dependent on CD40L mediated stimulation , whereas CD 86 and CD 95 expression was efficiently augmented by soluble factors released by preactivated T lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The B 7 family of molecules on antigen presenting cells , which include B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , play important roles in providing costimulatory signals required for development of antigen specific immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
During migration , DC upregulated the expression of major histocompatibility complex 2 and costimulatory receptors ( such as CD 40 , CD 80 , and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The authors observed that DC increased their expression of CD 80 , CD 86 , CD 40 , and human leukocyte antigen ( HLA ) DR and neoexpressed CD 83 , DC LAMP / CD208 , and CCR 7 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here we compared untreated MS , therapy with IFN beta alone and combined with GA , and healthy controls ( HC ) , regarding expression on HLA DR+ blood mononuclear cells ( MNC ) of CD1a that is a cell surface molecule with capacity to present glycolipids to T cells , and of CD 80 and CD 86 which are costimulatory molecules that activate Th 1 and Th 2 responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The latter DC population could be subdivided into a major , immature ( CD 40 ( lo ) CD 80 ( lo ) CD 86 ( lo ) MHC class 2 ( lo ) ) CD11c ( int ) subset , and a minor , mature ( CD 40 ( hi ) CD 80 ( hi ) CD 86 ( hi ) MHC class 2 ( hi ) ) CD11c ( hi ) subset . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Importantly , they become fully competent antigen presenting cells ( APC ) after a short maturation induced by IL 3 + CD40L or virus , exhibiting a characteristic APC phenotype ( high expression of CD 83 and of the costimulatory molecules CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells displayed morphological and phenotypic features of myeloid DC precursors with expression of HLA DR , HLA ABC , CD 86 , CCR 5 and CD 54 molecules but lacking CD 80 , CD 83 , CD1a and CD 40 antigens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Monoclonal antibodies were used to assess the expression of CD 80 , CD 86 , CD 68 , CD 14 , CD45RO , human leukocyte antigen DR ( HLA DR ) , and intercellular adhesion molecule 1 ( ICAM 1 ) in renal tissues . ^^^ CONCLUSION : This study suggested that CD 80 and CD 86 activate T cells in IgAN , CD80 / CD86 expressions correlated with renal function at the time of renal biopsy , and monocyte / macrophages and tubular epithelial cells act as APC . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
PGE ( 2 ) as well as 11 , 15 O dimethyl prostaglandin ( E ( 2 ) ONO AE 1 259 01 ) ( EP 2 receptor agonist ) and ONO AE 1 329 ( EP 4 receptor agonist ) concentration dependently enhanced the expression of CD 80 , CD 86 , CD 83 , and HLA DR on MoDCs during maturation , especially in the presence of TNF alpha , whereas 17S 2 , 5 ethano 6 oxo 17 , 20 dimethyl prostaglandin E ( 1 ) ( EP 1 receptor agonist ) and 16S 9 deoxy 9beta chloro 15 deoxy 16 hyfroxy 17 , 17 trimethylene 19 , 20 didehydro prostaglandin F ( 2 ) ( EP 3 receptor agonist ) showed no effect . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of CD1a , CD 80 , CD 86 in dendritic cell of tumor tissue and regional lymph node in esophageal carcinoma ] . ^^^ This study was designed to investigate the expression of CD1a ( specific marker ) and CD 80 , CD 86 ( costimulating molecules ) in dendritic cell of tumor tissue and regional lymph node of esophageal carcinoma and to investigate the mechanisms by which tumors escape immune recognition . ^^^ METHODS : The expression of CD1a , CD 80 , CD 86 in dendritic cells of tumor tissues and paratumor tissues from 58 patients with esophageal carcinoma ( clinical period 1 2 : 27 cases , 3 4 : 31 cases ; differentiation G ( 1 2 ) : 37 cases , G 3 : 21 cases ; squamous cancer : 49 cases , adenoid cancer : 9 cases ; lymph node metastases present : 37 cases , lymph node metastases absent : 21 cases ) , inflammatory tissues from 11 patients with esophagitis , 12 normal esophageal tissues ( as control ) , 31 regional lymph nodes with tumor and 34 regional lymph nodes without tumor were determined using flow cytometry . ^^^ CONCLUSION : ( 1 ) The number of dendritic cell of CD1a ( + ) decreases in tumor tissue and regional lymph node with tumor in esophageal carcinoma and the expression of CD 80 , CD 86 are down regulated . ( 2 ) The lower the tumor differentiation grade , the less the number of CD1a ( + ) dendritic cell in tumor tissue and regional lymph node and the lower the expression of CD 80 , CD 86 in tumor tissue . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Indirect recognition predominated the response , as antibodies against mouse class 2 1 A ( b ) , CD 80 , or CD 86 molecules significantly ( 45 to 60 % ) blocked the proliferative response . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The coculturing of iDCs with apoptotic cells did not induce the maturation of iDCs even after the subsequent LPS treatment , as assessed as to the expression of MHC class 2 , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Myeloid DC from hepatic LN showed similar expressions of HLA DR , CD 83 , and CD 86 , and higher expression of CD 80 compared to myeloid DC from inguinal LN . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulation of DCs with S . pyogenes resulted in strong expression of DC costimulatory molecules CD 80 , CD 83 , and CD 86 accompanied with a T helper cell type 1 ( Th 1 ) cytokine and chemokine response . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cross regulation of CD 86 by CD 80 differentially regulates T helper responses from Mycobacterium tuberculosis secretory antigen activated dendritic cell subsets . ^^^ We report that stimulation of Mycobacterium tuberculosis secretory antigen and tumor necrosis factor alpha matured BALB / c mouse bone marrow dendritic cells ( BMDCs ) with anti CD 80 monoclonal antibody up regulated CD 86 levels on the cell surface . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells expressed significant levels of CD 40 , CD 80 and CD 86 , and could stimulate allogeneic CD4+ T cells efficiently . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Finally , CD 80 and CD 86 , molecules involved in T B cooperation , showed an inverse expression between lymphocytes of B CLL patients and healthy subjects . ^^^ CD 80 was higher on normal than leukemic B cells , while CD 86 expression was higher on CLL B cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vitro study revealed that CD 80 , CD 86 and CD11b expression on macrophages was augmented after IL 1beta stimulation , whereas the up regulation could be blocked by sodium salicylate . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory molecules ( CD 80 , CD 86 , CD 28 , CD 152 ) , accessory molecules ( TCR alphabeta , TCR gammadelta ) and T cell lineage molecules ( CD4+ , CD8+ ) in PBMC of leprosy patients using Mycobacterium leprae antigen ( MLCWA ) with murabutide and T cell peptide of Trat protein . ^^^ This observation prompted us to study the expression of the costimulatory molecules ( CD 80 , CD 86 , CD 28 , CD 152 ) , other accessory molecules ( TCR alphabeta / gammadelta ) and T cell lineage molecules ( CD4+ and CD8+ ) during constitutive and activated state of peripheral blood mononuclear cells ( PBMC ) derived from normal and leprosy individuals using different formulations of Mycobacterium leprae total cell wall antigen ( MLCWA ) , Trat and MDP BE using flow cytometric analysis . ^^^ An increased surface expression of CD 80 , CD 86 and CD 28 but decreased CD 152 expression was observed when PBMC of normal , BT / TT ( tuberculoid ) and BL / LL ( lepromatous ) patients were stimulated in vitro with MLCWA+MDP BE+Trat peptide using liposomal mode of antigen delivery , while opposite results were obtained with the antigen alone . ^^^ Antibody inhibition study using antihuman CD 80 or CD 86 completely abolished the T cell lymphoproliferation , thereby reconfirming the importance of these costimulatory molecules during T cell activation / differentiation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that HOT stimulated DC to express the surface maturation markers CD 80 , CD 83 , and CD 86 dose dependently and that the up regulation level was identical to TNF alpha and LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have therefore investigated associations between MS and polymorphisms in the CD 152 ( CTLA 4 ) , CD 28 , CD 80 and CD 86 genes in Australian patients . ^^^ Screening of CD 28 , CD 80 and CD 86 genes identified novel polymorphisms in the putative promoter regions of CD 28 ( 372 G / A ) and CD 86 ( exon 2 359 deletionAAG ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CyaA also enhanced cell surface expression of CD 80 , CD 86 , and major histocompatibility class 2 on immature DC . ^^^ The stimulatory activity of our CyaA preparation for IL 10 production and CD 80 , CD 86 , and major histocompatibility complex class 2 expression was attenuated following the addition of polymyxin B or with the use of DC from Toll like receptor ( TLR ) 4 defective mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 86 and CD 80 differentially modulate the suppressive function of human regulatory T cells . ^^^ As both CD 28 and CTLA 4 molecules are implicated in the function of Treg , we investigated the ability of their two natural ligands , CD 80 and CD 86 , to influence the Treg suppressive capacity . ^^^ Intriguingly , the relative expression levels of CD 80 and CD 86 on dendritic cells are modulated during progression from an immature to a mature state , and this correlates with the ability of Treg to suppress responses . ^^^ Our data show that CD 80 and CD 86 have opposing functions through CD 28 and CTLA 4 on Treg , an observation that has significant implications for manipulation of immune responses and tolerance in vivo . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cultured bone marrow nucleated cells expressed dendritic cell markers including MHC class 2 antigen , CD 80 , CD 86 , and CD11c . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , inosine did neither influence membrane expression of CD 54 , CD 80 , CD 83 , CD 86 , HLA DR , and MHC class 1 molecules nor modulated secretion of interleukin ( IL ) 12 , IL 10 , and tumor necrosis factor alpha in immature and lipopolysaccharide matured DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory molecules , CD49D and CD 28 on T cells and CD 80 and CD 86 on monocytes , was unchanged . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After 7 d , the majority of cells showed DC like morphology , and expressed higher levels of CD 80 , CD 86 and HLA DR than fresh leukaemic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The results indicated that gamma irradiation reduced expression of CD 86 , CD 80 and HLA DR molecules on DC , especially CD 86 ( P=0 . 0072 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 , CD 86 ) and adhesive molecules ( eg . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Only TNF alpha stimulated EC induced the development of mature , allostimulatory DC with a high expression of CD 83 , HLA DR , CD1a , and costimulatory molecules like CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The immunosuppressive LCMV variant clone 13 ( Cl 13 ) infects DCs and interferes with their maturation and antigen presenting capacity as evidenced by a significant reduction in the surface expression of MHC class 1 , MHC class 2 , CD 40 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vitro stimulation of immature murine DC with MALP 2 resulted in the induction of maturation with up regulated expression of MHC class 2 , costimulatory ( CD 80 , CD 86 ) and adhesion ( CD 40 , CD 54 ) molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Resveratrol inhibited the expression of costimulatory molecules ( CD 80 and CD 86 ) , and major histocompatibility complex ( MHC ) classes 1 and 2 significantly , and had the same effect dose dependently on DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We observed a weak expression of costimulatory molecules ( CD 80 , CD 86 ) and an absence of inflammatory cytokines ( IFNgamma , TNFalpha , IP 10 ) and lymphocyte activation markers ( CD 25 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , these DCs expressed high levels of CD 40 , CD 80 , CD 86 , and MHC class 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
ICOS binds to a specific ligand on antigen presenting cells ( APC ) and cells of the peripheral tissue different from the CD28 / CD152 ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Most of the DCs express immunocytochemically detectable antigens like : S 100 , CD1a , CD 40 receptor , adhesion molecules ( ICAM 1 or CD 54 , LFA 1 and LFA 3 ) , integrins ( CD11a , CD11c and CD 18 ) , CD 45 , CD 54 , co stimulatory molecules ( B 7 1 or CD 80 , B 7 2 or CD 86 ) , F 418 , MHC class 1 and 2 and DEC 205 , multilectin receptor , immunostimulatory cytokine ( IL 12 ) and , of course , Fc and complement receptors . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Nave CD 4 ( + ) T cells require two signals for proper activation and induction of differentiation : signal 1 is provided by peptide antigens in the context of the major histocompatibility complex ( MHC ) class 2 , while signal 2 is delivered by costimulatory molecules such as CD 80 or CD 86 present on the antigen presenting cell ( APC ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using splenic T cells and IPE from donor mice with disrupted genes for CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , CTLA 4 , and / or CD 28 , we report that B 7 2 ( + ) IPE in the presence of anti CD 3 supported selectively the activation of CTLA 4 ( + ) CD 8 ( + ) T cells that express their own B 7 2 and secrete enhanced amounts of active TGFbeta . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immature dendritic cells with characteristic phenotypic markers CD 40 , CD 80 , and CD 86 were successfully isolated from the blood of patients with pancreatic cancer . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This may be due to the direct effect of decreased Tax expression , because the Tax siRNA did not alter the expression of MHC 1 , CD 80 , or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : The expression of CD 83 , CD 80 , and CD 86 on the surface of dendritic cells cultured from the peripheral blood of patients was detected compared with that from health donors using flow cytometry . ^^^ The expression of CD 80 , CD 86 , CD 83 , and S 100 proteins was assessed in esophageal carcinoma tissues using immunohistochemical method . ^^^ RESULTS : Compared with those from healthy donors , dendritic cells cultured from the peripheral blood of patients expressed lower CD 80 and CD 86 . ^^^ Compared with the control group , the positive expression ratio and frequencies of CD 80 , CD 86 , and S 100 in esophageal carcinoma tissues were significantly down regulated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After up regulation of the C3aR by IFN alpha+ IFN gamma , C3a significantly up regulated the surface expression of CD 54 , CD 83 and CD 86 , but not of CD 40 , CD 80 or human leucocyte antigen ( HLA ) DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Markers of DC activation CD 80 and CD 86 were absent from both groups of specimens . ^^^ Flow cytometry analysis of the effects of C . pneumoniae infection on immature monocyte derived DCs in vitro showed no changes in the expression of CD1a , MHC class 2 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of CD 80 , CD 83 , CD 86 , CD1a , and CD 40 on leukemic DCs was significantly lower than that on donor DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules ( CD 80 and CD 86 ) , not associated constitutively with rafts , were expressed at much lower level in A 20 cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The resulting adenovirus transduced DCs differentiated in the presence of monocyte conditioned medium and poly [ 1 ] : poly [ C ] , expressing the surface markers of mature DCs , including CD 83 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Analysis of DC from Flt 3L treated mice revealed an immature phenotype with low or absent expression levels of CD 80 , CD 86 and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present study , blasts from 21 patients with AML were cultured in vitro for 2 days , then cells were gamma irradiated and antigen presenting cell ( APC ) characteristics were assessed . gamma Irradiation induced expression of several characteristics of APCs in AML blasts , including expression of CD 80 , CD 86 , and BDCA 4 , and were stimulators of allogeneic mixed lymphocyte reactions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RTDCs isolated by enzyme digestion , Percoll density gradient centrifugation and overnight GM CSF culture followed by MACS separation retain an archetypical immature dendritic cell phenotype , characterised by MHCII ( low ) CD 40 ( neg ) CD 86 ( neg ) CD 80 ( neg ) CD11c ( low ) cell surface expression . ^^^ Splenic derived DC ( SDC ) isolated conformed to a day 1 in vitro phenotype ; MHCII ( low ) CD 40 ( neg ) CD 86 ( low ) CD 80 ( neg ) CD11c ( low ) but can further mature phenotypically in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , we collected Mo DCs from the collagen gels by collagenase treatment and analyzed the expression of antigen presentation related molecules such as HLA DR , CD 80 , CD 83 , and CD 86 on Mo DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The ingestion of MS did not change the cell surface expression of CD 80 , CD 83 , CD 86 and HLA DR of immature and mature DC , suggesting that MS uptake did not induce DC maturation but that maturation by cytokines or LPS was unaltered in the presence of MS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These activated platelets induced DC maturation , as revealed by significant up regulation of CD 83 , CD 80 , and CD 86 Ags . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study we demonstrate the induction of anergic regulatory T cells during an MLR performed in the presence of blocking mAb to the costimulatory molecules CD 40 , CD 80 , and CD 86 . ^^^ Thus , a subtype of regulatory T cells in human blood can be activated in the absence of costimulatory signals from CD 40 , CD 80 , and CD 86 , and they can be identified by expression of ICOS after activation . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the spleen , Ag bearing cells were CD 1 ( + ) , CD11b ( + ) , ED 1 ( + ) , ED 2 ( low ) , ED 3 ( + ) , CD 86 ( low ) , OX 6 ( + ) , CD11c ( ) , ED 5 ( ) and in the LNs were CD 4 ( + ) , CD 8 ( + ) , CD 80 ( + ) , and OX 41 ( + ) suggesting these were lymphoid organ resident macrophages . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 antigen expression was upregulated on B cells but not T cells during incubation with trimeric human CD 40 ligand ( huCD40LT ) + IL 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In these studies , we demonstrate that exogenous soluble LIGHT or soluble CD 40 ligand ( CD40L ) can promote monocyte derived dendritic cell maturation in vitro by the up regulation of CD 86 , CD 80 , CD 83 , and HLA DR antigen expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Adenovirus serotype 3 utilizes CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) as cellular attachment receptors . ^^^ Herein we present data indicating that adenovirus ( Ad ) serotype 3 utilizes CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) as cellular attachment receptors . ^^^ CD 80 and CD 86 are co stimulatory molecules that are present on mature dendritic cells and B lymphocytes and are involved in stimulating T lymphocyte activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show that isolated NSCs from adult brain express CD 80 and CD 86 ( B 7 2 ) and this expression is increased after exposure to IFN gamma or TNF alpha , the prototypical Th 1 cytokines expressed during EAE . ^^^ CD 80 and CD 86 expressed by NSCs are functional and can costimulate allogeneic cells in a mixed lymphocyte reaction . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
LPS inhibited APC maturation assessed by CD 80 and CD 86 expression , and inhibited chemokine ( CCL 3 and CCL 5 ) production . ^^^ LPS on CD 80 , CD 83 , and CD 86 , and on CCL 3 and CCL 5 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD1a , HLA DR and CD 54 were highly expressed on DC stimulated with both kinds of LPS ; however , CD 40 , CD 80 , CD 83 and CD 86 expression on Pg LPS stimulated DC was lower than on Ec LPS stimulated DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
HCs upregulate surface expression of major histocompatibility complex ( MHC ) class 1 molecules and CD1d but not MHC class 2 molecules Qa 1 , CD 80 , CD 86 , CD 54 , or CD 95 ; in addition , they expressed / secreted interleukin ( IL ) 10 and IL 4 but not IL 1 , IL 6 , IL 13 , interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) , IL 4 , or IL 27 ( i . e . , they acquire the HC * phenotype ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The most widely comprehended costimulatory pathway involves the ligation of CD 28 expressed on T cells by CD 80 and CD 86 upregulated on APCs . ^^^ We found that regardless of the maturation stage , CD 80 and CD 86 were evident within the plaques , and colocalized with the presence of markers of dendritic cells and with expression of the extensively investigated autoantigen oxidized LDL . ^^^ FACS analysis studies showed that splenocytes from aged atherosclerotic apoE KO mice exhibited increased expression on B cells ( which represent APCs ) of CD 80 and CD 86 as compared to their young apoE or nave C57BL / 6 litters that have no plaques . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , these cells fail to express conventional costimulatory molecules ( CD 80 , CD 86 ) , leading to the possibility that antigen presented by normal IECs could result in anergy . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
When macrophages were primed with IFN gamma , resveratrol suppressed the expression of HLA ABC , HLA DR , CD 80 , CD 86 and inhibited production of TNF alpha , IL 12 , IL 6 and IL 1beta induced by LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Co stimulatory molecules B 7 1 ( CD 80 ) / B7 2 ( CD 86 ) and their counter receptors CD28 / CTLA 4 play pivotal roles in T cell activation and immune regulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this syndrome , CD154 / CD40 interactions are required for but do not mediate disease by upregulation of CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
But CD 80 and CD 86 were expressed at low levels or absent on IL 3 treated DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The GPA ( ) cells were comprised of cells with excentric cell shape and were positive for HLA class 1 , HLA class 2 , CD1a , CD 4 , CD11c , CD 14 , CD 40 , CD 80 , CD 83 , and CD 86 , but not for CD 3 , CD 8 , CD 19 , CD 20 , and CD 56 , indicating the codevelopment of dendritic cells ( DC ) along with erythroid differentiation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Because freshly prepared spleen B cells are highly sensitive to irradiation , we employed spleen B cells stimulated with lipopolysaccharide ( LPS B cells ) . 10 irradiation ( 8 Gy ) followed by incubation ( 9 12 hr ) highly and selectively up regulated CD 80 expression in LPS B cells , whereas the same treatment slightly increased expression of CD 54 and did not affect expression of CD 86 , major histocompatibility complex class 2 , CD11a or surface immunoglobulin M . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Co treatment of PA with LPS further increased dendritic cell surface marker expressions ( CD 80 , CD 86 , CD 40 , MHC class 1 , and class 2 antigens ) and reduced the phagocytic activity of LPS treated cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vivo experiments in the mouse DA 1 3b / C3H whole animal acute myeloid leukemia ( AML ) model showed that injection of Ara C induced expression of CD 80 and CD 86 , and decreased expression of B 7 H1 , indicating that chemotherapy can modify costimulatory molecule expression in vivo , in a way not necessarily observed in vitro . ^^^ Ara C also induced CD 80 or CD 86 expression in 14 of 21 primary cultured human AML samples . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The B 7 ligands CD 80 and CD 86 on APCs deliver either costimulatory or inhibitory signals to the T cell when interacting with their counter receptors CD 28 and CD 152 ( CTLA 4 ) on the T cell surface . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The results showed that DCs cultured in the presence of MPA expressed low levels of CD 40 , CD 80 and CD 86 , and exhibited weak activity in stimulating the proliferation of allogeneic T cells and antigen presenting function with a concurrent reduction of IL 12 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Chronic ethanol ingestion by mice increases expression of CD 80 and CD 86 by activated macrophages . ^^^ We now report that in vitro activated CD11b ( + ) splenocytes obtained from C57BL / 6 and BALB / c mice that consumed ethanol chronically express increased levels of the T cell co stimulatory molecules CD 80 and CD 86 . ^^^ Evaluation of cultures of purified CD11b ( + ) cells , obtained from mice that consumed ethanol chronically , incubated overnight , showed increased up regulation of CD 80 and CD 86 expression on Gr . 1 ( ) mouse splenic macrophages . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Insertion of host derived costimulatory molecules CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) into human immunodeficiency virus type 1 affects the virus life cycle . ^^^ Two major cell types that harbor HIV 1 in vivo , i . e . , monocytes / macrophages and CD4+ T cells , express the costimulatory molecules CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) . ^^^ We investigated whether CD 80 and CD 86 are efficiently acquired by HIV 1 , and if so , whether these host encoded molecules can contribute to the virus life cycle . ^^^ Here we provide the first evidence that the insertion of CD 80 and CD 86 into HIV 1 increases virus infectivity by facilitating the attachment and entry process due to interactions with their two natural ligands , CD 28 and CTLA 4 . ^^^ Moreover , we demonstrate that NF kappaB is induced by CD 80 and CD 86 bearing virions when they are combined with the engagement of the T cell receptor / CD3 complex , an event that is inhibited upon surface expression of CTLA 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recently we reported that high potency ( CMRF 44 ( + ) ) dendritic cells ( DC ) in the peripheral blood of patients with MM failed to significantly up regulate the expression of the B 7 co stimulatory molecules , CD 80 and CD 86 , in response to an appropriate signal from soluble trimeric human CD 40 ligand . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Monocyte staining showed significant increases in human leukocyte antigen D related , CD 54 , CD 80 , and CD 86 surface markers in intervillous blood , compared with peripheral blood , which suggests a relative activation of monocytes in the placenta . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometry showed that day 5 dendritic cells ( DC ) were less mature than day 7 DC as indicated by the expression of CD1a , CD11c , CD 14 , CD 80 , CD 83 , CD 86 and MHC 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recombinant ( r ) PorA induced mo DC maturation , as reflected by reduced receptor mediated endocytosis , increased production of the chemokines IL 8 , RANTES , MIP 1 alpha and MIP 1 beta and augmented expression of the surface markers CD 40 , CD 54 , CD 80 , CD 86 and major histocompatibility complex class 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results show that MSCs inhibit the up regulation of CD1a , CD 40 , CD 80 , CD 86 , and HLA DR during DC differentiation and prevent an increase of CD 40 , CD 86 , and CD 83 expression during DC maturation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Memory B cells in SSc showed increased expression of activation markers , including CD 80 , CD 86 , and CD 95 , relative to normal controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 10 modified DC resulted in lower clinical scores , less body weight loss , lower numbers of anti AChR IgG antibody secreting cells and lower affinity of anti AChR antibodies in rats receiving IL 10 modified DC , accompanied with lower expression of CD 80 and CD 86 and lower lymphocyte proliferation among lymph node mononuclear cells compared with control EAMG rats . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We also observed that at the molecular level Tomatine required both CD 80 and CD 86 costimulation to engender antigen specific cellular immunity . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The role of B 7 family members CD 80 and CD 86 in providing costimulatory signals to T cells is well established . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition to cytokine induction , TLR activation by fimbriae also results in upregulation of the CD 40 , CD 80 , and CD 86 costimulatory molecules in antigen presenting cells , suggesting that fimbriae are sensed as a potential `` danger ' ' to the host immune system . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Generation of tolerogenic dendritic cells by treatment with mitomycin C : inhibition of allogeneic T cell response is mediated by downregulation of ICAM 1 , CD 80 , and CD 86 . ^^^ MMC treatment selectively decreases adhesion ( intercellular adhesion molecule [ ICAM ] 1 ) and co stimulatory ( CD 80 , CD 86 ) molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD11c ( approximately 90 % ) , MHC 2 ( approximately 70 % ) , CD 86 ( approximately 20 % ) , CD 83 ( approximately 5 % ) , CD 80 ( approximately 40 % ) , CD 40 ( approximately 40 % ) , and CCR 7 ( approximately 5 % ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
By a direct flow cytometry approach , the blood levels of circulating lineage ( CD 3 , CD 19 , CD 14 ) negative , CD33++ , HLA DR+ cells were estimated before and following ex vivo cell differentiation , and phenotyped by MAbs with specificity against HLA DR , HLA ABC , CD1a , CD11c , CD 33 , CD 40 , CD49d , CD49e , CD 54 , CD 80 , CD 83 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The performance of DCs in mixed lymphocyte reaction correlated significantly ( p = 0 . 005 ) with the CD 83 expression but not with the CD 80 , CD 86 , HLA DR , CD1a , and CD1c expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , when HL 60 cells were treated with A 23187 for 96 hours , the expressions of CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , MHC class 2 molecule and CD 54 on HL 60 cells reached peak , and marked activation of allogeneic T cells occurred . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Importantly , activation of CTL by OVA CT pulsed DC was dependent on CT induced activation of adenylate cyclase and increased cAMP production by DC associated with increased expression of MHC class 1 and co stimulatory molecules ( CD 80 , CD 86 and CD 40 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Liver sinusoidal endothelial cells ( LSEC ) have been reported to express MHC class 2 , CD 80 , CD 86 , and CD11c and effectively stimulate naive T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Monocytes exposed to EBV became larger in size with increased granularity and expressed considerably higher levels of membrane HLA classes 1 and 2 , ICAM 1 , CD 80 , CD 86 , and CD 40 compared with uninfected cultures . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The gammadelta T cell effect upon the LNC response to MBP did not involve a change in expression of the costimulatory molecules CD 28 , CD40L , and CTLA 4 on TCRalphabeta ( + ) cells , and CD 40 , CD 80 , and CD 86 on CD 19 ( + ) and CD11b ( + ) cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
HSP60 priming of monocytes also results in significant down regulation of HLA DR , CD 86 and Toll like receptor 4 expression , but minimally up regulates CD 80 expression , similar to that previously reported with LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
LPS stimulation resulted in a dose dependent up regulation of CD 40 , CD 80 , and CD 86 on monocytes , and up regulation of CD 69 on NK cells and gamma delta T cells in both the periodontitis and non periodontitis groups . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The SR A contributes to phagocytosis of the organisms but not to their ability to induce CD 80 , CD 86 , and MARCO or to inhibit MHC 2 . ^^^ Conversely , studies with lipopolysaccharide ( LPS ) deficient organisms and / or TLR 4 mutant mice showed that LPS and TLR 4 are at least partially required to induce CD 80 , CD 86 , and MARCO , but LPS is not required to inhibit MHC 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expressions of CD 80 , CD 86 and HLA DR on DC were slightly increased . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Lower percentages of monocytes with CD 80 , CD 86 and HLA DR molecule expression in pediatric cancer . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Transfection of IFN gamma gene into DC promoted DC to express higher CD 40 , CD 54 , CD 80 , CD 86 , CCR 7 and 1 Ab , secrete more IL 1beta and IL 12p70 , and more potently activate both CD 4 and CD 8 T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Tacrolimus and steroid ointment induced a selective depletion of IDECs from the epidermis and reduced the expression of the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The effect of TNF alpha neutralisation on the phenotype ( CD 14 , CD 16 , CD 32 , CD 64 , CD 80 , CD 83 , CD 86 , and MHC ) of dendritic cells was investigated by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Dendrimer glucosamine inhibited Toll like receptor 4 mediated lipopolysaccharide induced synthesis of pro inflammatory chemokines ( MIP 1 alpha , MIP 1 beta , IL 8 ) and cytokines ( TNF alpha , IL 1 beta , IL 6 ) from human dendritic cells and macrophages but allowed upregulation of the costimulatory molecules CD 25 , CD 80 , CD 83 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 27 ( + ) IgD ( ) memory B cells , i . e . , cells with hyper mutated IgV genes , were significantly increased in the CSF vs . paired peripheral blood and displayed up regulation of the CD 80 and CD 86 costimulatory molecules and of CC chemokine receptor ( CCR ) 1 , CCR 2 , and CCR 4 in both patient groups . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting analysis of cell surface expression of maturation marker molecules such as CD 80 , CD 83 , CD 86 , and HLA DR revealed equal upregulation after stimulation with H . pylori or LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NF kappaB binding activities , costimulatory molecule ( CD 40 , CD 80 , CD 86 ) surface expression , IL 12 protein expression and allostimulatory capacity of DCs were measured with electrophoretic mobility shift assay ( EMSA ) , flow cytometry , Western blotting , and mixed lymphocyte reaction ( MLR ) , respectively . ^^^ RESULTS : GM CSF propagated DCs , GM CSF+NF kappaB decoy ODNs propagated DCs and GM CSF+ scrambled ODNs propagated DCs exhibited features of immature DCs , with similar low level of costimulatory molecule ( CD 40 , CD 80 , CD 86 ) surface expression , absence of NF kappaB activation , and few allocostimulatory activities . ^^^ GM CSF+IL 4 propagated DCs displayed features of mature DCs , with high levels of costimulatory molecule ( CD 40 , CD 80 , CD 86 ) surface expression , marked NF kappaB activation , and significant allocostimulatory activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OK PSA treatment effectively increased the surface expression of MHC class 2 , CD 80 , CD 83 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) were induced by listerial infection only when the bacteria invaded the cytoplasm . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , CTL responses were associated with a partial activation of neonatal CD11c ( + ) DC , reflected by the up regulation of CD 80 and CD 86 expression but no concurrent changes in MHC class 2 or CD 40 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
All mature DCs uniformly expressed comparable levels of HLA DR , CD 83 , CD 80 , and CD 86 , and were potent stimulators of allogeneic T cells after exposure either to recombinant human CD40L trimer or a combination of inflammatory cytokines with PGE ( 2 ) . moDCs , however , required 0 . 5 1 log greater numbers than LCs or DDC IDCs to stimulate comparable T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : DCs up regulated the expression of CD1a ( 55 . 0 % ) , CD11c ( 98 . 6 % ) , CD 86 ( 86 . 1 % ) , CD 80 ( 66 . 1 % ) , and HLA DR ( 88 . 9 % ) after cultured for 5 days . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , unlike EBOV and MARV , VLPs induced maturation of DCs including upregulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , major histocompatibility complex ( MHC ) class 1 and 2 surface antigens , and the late DC maturation marker CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Following 6 days in culture , four of five cell lines demonstrated dendrite like morphology , upregulation of CCR 7 , CD 54 , CD 80 and CD 86 , uptake of extracellular proteins and activation of T cells , and similar results were obtained with blasts from three t ( 9 ; 22 ) ALL patients . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CpG ODN induced enhancement of CHS was due to increased antigen presenting cell functions of DC since : ( 1 ) CpG ODN injected skin revealed upregulated expression of major histocompatibility complex class 2 , CD 80 , and CD 86 molecules and ( 2 ) CpG ODN treatment of DNFB derivatized DC enhanced the intensity of CHS responses after in vivo transfer . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , a distinct population of CD11c ( + ) MHCII ( high ) DC showing low expression of the costimulatory molecules CD 40 , CD 80 , and CD 86 in wild type animals was virtually absent in skin draining lymph nodes of CCR 7 deficient mice under steady state conditions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that mice nasally administered with Stx 1 B or mStx 1 showed an up regulation in the expression of CD 80 , CD 86 and especially CD 40 on NALT DCs . ^^^ Taken together , these results suggest that non toxic Stx derivatives could be effective mucosal adjuvants for the induction of Th 2 type , CD4+ T cell mediated , antigen specific mucosal IgA and systemic IgG Ab responses , and that they likely owe their adjuvant activity to the up regulation of co stimulatory molecules including CD 80 , CD 86 and CD 40 on NALT DCs . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Comparing the surface induction of CD 83 with the upregulation of CD 40 , CD 80 and CD 86 during LPS ( lipopolysaccharide ) induced DC maturation showed that CD 83 induction occurred more rapidly . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
PS liposomes inhibited the up regulation of HLA ABC , HLA DR , CD 80 , CD 86 , CD 40 , and CD 83 , as well as the production of IL 12p70 by human DCs in response to LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Alcohol treated DCs showed reduced IL 12 , increased IL 10 production , and a decrease in expression of the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of the surface molecules CD 80 , CD 86 , HLA DR etc . on the DC was examined by fluorescent activated cell sorter ( FACS ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 10 induced CD 14 ( + ) CD 16 ( ++ ) cells that expressed dendritic cell markers : CD 80 , CD 86 , HLA DR , and CD 83 and initiated significantly decreased allogeneic mixed lymphocyte reactions ( MLRs ) . ^^^ Blockage of CD 86 , but not CD 80 , further down modulated MLRs induced by CD 14 ( + ) CD 16 ( ++ ) cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , in parallel with their differential expression of costimulatory molecules CD 80 and CD 86 and lack of IL 12 , nicDCs display profoundly reduced Th 1 promoting capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , the expression of maturation markers such as MHC 2 , CD 40 , CD 80 and CD 86 , and of differentiation markers such as CD 4 , CD 8 and CD11b , was also comparable in the two populations . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The adaptive immune response is dependent upon upregulation of costimulatory molecules ( UCM ) such as CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
TgHSP 70 induced the maturation of human monocyte derived dendritic cells as determined by increased levels of surface markers , namely , CD 40 , CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Culture of PBMC with alum induced increased expression of CD 86 ( P=0 . 004 ) and HLA ( P=0 . 01 ) on monocytes while the expression of CD 80 was decreased ( P=0 . 02 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Accordingly , a decreased percentage of mDC expressing CD 80 and CD 86 was observed after maturation , compared to controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
GCP 2 increased CD 80 and CD 86 expression on B cells during in vitro stimulation in a dose dependent manner . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The increase in costimulatory ( CD 80 and CD 86 ) and adhesion ( ICAM 1 , PGP 1 , LFA 1 , and Mac 1 ) in peripheral blood mononuclear cells was also attenuated by food restriction and to a lesser extent by fish oil alone . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mature DC are characterized by active surface expression of MHC and co stimulatory molecules ( MHC 1 , MHC 2 , CD 40 , CD 80 , CD 86 ) . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Compared to immature DC ( imDC ) cultured by GM CSF alone , the TGFbeta DC express lower CD 80 , CD 86 , 1 Ab and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : PBMCs of the healthy donor cocultured with rhGM CSF plus CI for 40 hours had the typical morphology of DCs , with decreased CD 14 expression , and increased CD 83 , CD 80 and CD 86 expressions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , unlike DC , MP were early and strongly activated in response to MV , as shown by the increased surface expression of CD 86 , CD 80 , CD 54 , CD 40 , and HLA abc and by the production of mRNA encoding alpha interferon ( IFN alpha ) , IFN beta , tumor necrosis factor alpha and interleukin 6 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Also , PL enhanced the expression of costimulatory molecules , CD 80 and CD 86 , and major histocompatibility complex ( MHC ) molecules 2 in PM . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Murine bone marrow derived DCs on culture with Bryo 1 alone , Bryo 1 + calcium ionophore ( CI ) , but not CI alone exhibited morphologic changes characteristic of mature DCs and expressed increased levels of CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : Twenty eight HCC samples obtained by surgical resection were analyzed for the expression of beta 2 microglobulin , heat shock protein ( HSP ) 70 , human leukocyte antigen ( HLA ) class 1 , CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) and IL 10 by immunostaining . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that lycopene down regulates the expression of costimulatory molecules ( CD 80 and CD 86 ) and major histocompatibility complex type 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These mPhi showed increased production of interferon gamma ( IFNgamma ) , exclusive production of interleukin 18 ( IL 18 ) and increased expression of major histocompatibility complex ( MHC ) class 2 , CD 40 , CD 80 and CD 86 , when compared with thioglycolate induced mPhi . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of the B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules on the surface of leukemia cells made unnecessary the additional CD 28 costimulation of T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypically `` immature ' ' dendritic cells ( DCs ) , defined by low cell surface CD 40 , CD 80 , and CD 86 can elicit host immune suppression in allotransplantation and autoimmunity . ^^^ CD 40 , CD 80 , and CD 86 cell surface molecules were specifically down regulated by treating NOD DCs ex vivo with a mixture of antisense oligonucleotides targeting the CD 40 , CD 80 , and CD 86 primary transcripts . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Finally , we show that donor corneal APC demonstrate high expression of CD 40 , CD 80 , and CD 86 costimulatory molecules when derived from HR but not LR grafts . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NB 4 cells up regulated markers found in DCs , including HLA DR , costimulatory molecules ( CD 80 and CD 86 ) , adhesion molecules ( CD 40 ) , and chemokine receptors ( CCR 6 ) when cultured for 8 days in the presence of 1 microM ATRA . ^^^ CD11b was coexpressed with CD 80 , CD 83 , and CD 86 in ATRA treated NB 4 cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , the costimulatory signal of CD 86 was selectively blocked , and that of CD 80 was kept unimpaired by administration of anti murine CD 86 monoclonal antibody at the early gestational stage in abortion prone CBA / JxDBA / 2 matings and normal pregnant CBA / JxBALB / c matings . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results show that LTA and MA regulate expression of CD 86 on macrophages while the expression of CD 80 remains unmodified . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Estrogen did not affect the expression of MHC class 2 , CD 80 and CD 86 by DC , but inhibited the ability of DC to stimulate T cell proliferation and production of both Th 1 and Th 2 cytokines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NES , but not heat inactivated NES , up regulates DC maturation markers associated with Th 2 promotion ( CD 86 and OX40L ) , with little change to CD 80 and MHC class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
PNIPAAm g PP membranes containing adsorbed anti mouse CD 80 monoclonal antibody preferentially captured mouse CD 80 transfected cells at 37 degrees C compared with membranes lacking antibody or containing anti mouse CD 86 monoclonal antibody . ^^^ With this method , mouse CD 80 or mouse CD 86 transfected cells were enriched from a 1 : 1 cell suspension to 72 % or 66 % , simply and with high yield . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
SP 600125 , a specific inhibitor of JNK , inhibited the LPS induced up regulation of CD 80 , CD 83 , CD 86 and CD 54 , but augmented the up regulation of HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This FcRH 1 down regulation coincides with dramatic enlargement of the pre germinal center cells , cell cycle entry , and other overt signs of activation that include CD 80 and CD 86 up regulation and immunoglobulin D ( IgD ) down regulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In comparison to immature Mo DC that were derived by culture with GM CSF and IL 13 only , CD 40 , CD 80 , CD 86 , HLA ABC and HLA DR molecules were up regulated upon OM 197 or LPS treatment similarly . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 expression got enhanced on the B 1a cells whereas CD 86 got solely expressed on B 1b cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BCG treated DCs enhanced significantly the expressions of CD 80 , CD 86 , and CD 40 , and the productions of IL 12 and IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The AML cells showed clear upregulation of CD 80 , CD 83 , CD 86 , CD 40 , and HLA DR expression under all culture conditions , without significant differences between these groups . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A number of receptor / ligand pairs have been shown to mediate costimulation , including CD28 / B7 molecules ( CD 80 and CD 86 ) , CD40 / CD40 ligand ( CD40L , CD 154 ) , and LFA 1 ( CD 18 ) / ICAM 1 ( CD 54 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , we demonstrated that our monoclonal antibodies , such as 9H10 and 4C12 , could trigger OX40L reverse signal that enhanced IgG production of B cells and promoted maturation of DCs as evidenced by the upexpression of CD 80 , CD 86 , CD 83 and CXCR 4 and monoclonal antibody 9H10 could also promote anti CD 40 monoclonal antibody stimulated DCs in order to induce T cells to secrete more interleukin 2 and interferon gamma , which suggested that OX40L signals could strengthen the effect of CD 40 signals on promoting Th 1 differentiation . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 28 induces immunostimulatory signals in dendritic cells via CD 80 and CD 86 . ^^^ Production of interleukin 6 required B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) and p 38 mitogen activated protein kinase and prevented interferon gamma driven expression of immunosuppressive tryptophan catabolism . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Before culture , cells did not express CD1a , CD 80 and CD 83 , while expressed CD 54 , CD 86 and HLA DR with low frequency . ^^^ After culture , cells upregulated CD1a , CD 80 , CD 83 , CD 54 , CD 86 and HLA DR significantly . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The ratio of CD8+ DC to CD 8 DC increased , and PG enhanced IL 12 and IFN gamma production , and expression of surface molecules including major histocompatibility complexes ( MHC ) classes 1 , MHC 2 , CD 80 , and CD 86 in MCA 102 challenged mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of CD 80 and CD 86 was enhanced by IFNgamma treatment but was not modified by IL 1beta ; the expression of CD 86 and HLA DR was reduced by TGFbeta 1 treatment . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The aim of the study was to estimate the expression of CTLA 4 ( cytolitic T lymphocyte associated antigen 4 , CD 152 ) , CD 28 , B7 . 1 ( CD 80 ) and CD7 . 2 ( CD 86 ) molecules on peripheral blood cells in patients with Graves ' disease ( GD ) ( n=28 , mean age 15 . 4 ) , in patients with nontoxic nodular goiter ( NTNG ) ( n=28 , mean age 15 . 6 years ) in comparison with sex and age matched healthy control subjects ( n=28 , mean age 15 . 9 years ) . ^^^ The analysis of CD3+ T lymphocytes co expressing CD 152 and CD 28 antigens on peripheral blood revealed increased percentages of CTLA 4 / CD28 positive cells in patients with Graves ' disease ( p < 0 . 004 , p < 0 . 04 ) compared to the controls and euthyroid Graves ' patients , while B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) molecules were detected only in some hyperthyroid patients on activated monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 86 expression was significantly enhanced ( P < 0 . 05 ) by stimulation with either CD40L AP ( 75 . 5+ / 14 . 5 % ) or sCD40L ( 80 . 5 % + / 5 . 3 % ) compared with immature DC ( 55 . 2+ / 14 . 8 % ) , as were CD 80 and CD 83 . ^^^ Similarly , in large scale studies using Isolex 300I to enrich for monocytes and platelets for DC generation / maturation on a clinical scale , stimulation with CD40L AP increased CD 86 and CD 80 expression as well as the ability to stimulate allogeneic lymphocytes compared with control cultures . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Lentiviral transduction of DCs resulted in down regulation of cell surface molecules including CD1a , co stimulatory molecules CD 80 , CD 86 , ICAM 1 , and DC SIGN . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Incubation of immature human PBMC derived DCs with SHIV VLPs for 48 h resulted in the significant up regulation of CD 40 , CD 80 , CD 83 , CD 54 , CD 86 , HLA A , B , C and HLA DR , DP , DQ molecules on activated DC CD11c+ subpopulations . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , the expression of HLA DR , CD 80 and CD 86 , was significantly reduced on rhIL 10 treated dendritic cell subsets . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The FastDCs and novel dendritomas were immunostained with anti CD 80 , anti CD 86 , anti CD 83 , anti HLA DR mAbs and analyzed by fluorescence activated cell sorting ( FACS ) . ^^^ RESULTS : Mature FastDCs with highly expressed surface markers CD 80 , CD 86 , CD 83 and HLA DR were generated within 48 h in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 , but not CD 154 , augment DNA vaccine induced protection in experimental bovine tuberculosis . ^^^ The co administration of costimulatory molecules CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) and CD 154 ( CD40L ) has been shown to enhance immune responses in several murine models . ^^^ In these studies , we demonstrate that the co administration of CD 80 and CD 86 , but not CD 154 , to an existing candidate subunit DNA vaccine ( ESAT 6 ) against bovine tuberculosis , enhances protection after aerosol challenge with virulent Mycobacterium bovis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast to LMP 1 , LMP1CD40 caused an upregulation of CD 69 , CD 80 , and CD 86 in B cells and a dramatic increase in serum immunoglobulin M . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting revealed that XS 106 IDO DCs were not changeable for Ia , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interferon beta up regulates the expression of co stimulatory molecules CD 80 , CD 86 and CD 40 on monocytes : significance for treatment of multiple sclerosis . ^^^ Peripheral blood mononuclear cells ( PBMCs ) were stimulated with IFN beta in vitro and expression of CD 80 , CD 86 , CD 40 and HLA was examined by flow cytometry and reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Concomitantly with HTLV 1 expression , these ATL cells expressed co stimulatory molecules such as CD 80 , CD 86 and OX 40 , and showed elevated levels of antigenicity against allogeneic T cells and HTLV 1 Tax specific cytotoxic T lymphocytes ( CTL ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the in vitro experiments , OK PSA enhanced expression of MHC class 2 , CD 80 and CD 86 , as well as IL 12 production on dendritic cells ( DCs ) were derived from wild type mice , but not from TLR 4 deficient ( TLR 4 / ) mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expressions of CD 80 , CD 86 were lower on TGFbeta DC than on imDC [ ( 4 . 14 + / 0 . 95 ) % vs ( 13 . 90 + / 7 . 22 ) % ; ( 8 . 60 + / 0 . 75 ) % vs ( 20 . 63 + / 5 . 03 ) % , P < 0 . 05 , both ] . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Infection of DC with Y . enterocolitica wild type resulted in decreased up regulation of major histocompatibility complex class 2 , CD 54 ( intercellular adhesion molecule 1 ) , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The inactivation of the MAPK pathway in both macrophages and dendritic cells leads to inhibition of proinflammatory cytokine secretion , downregulation of costimulatory molecules such as CD 80 and CD 86 , and ineffective T cell priming . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We were able to demonstrate that AAV / BA46 / Neo loading of DCs resulted in : ( 1 ) BA 46 expression in DCs , ( 2 ) chromosomal integration of the AAV / BA46 / Neo vector within DCs , ( 3 ) strong , rapid BA 46 specific , MHC class 1 restricted CTLs in only 1 week , ( 4 ) T cell populations with significant interferon gamma ( IFN gamma ) expression but low IL 4 expression , ( 5 ) high CD 80 and CD 86 expression in DCs , and ( 6 ) high CD 8 : CD 4 and CD 8 : CD 56 T cell ratios . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 induction by IL 2 were positively regulated through the mitogen activated protein kinase pathway , while CD 86 expression was negatively regulated through Janus kinase pathways . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These DC s express MHC 2 , the costimulatory molecules CD 40 , CD 80 , CD 86 , adhesion molecules such as DC SIGN and chemokine receptors such as CCR 7 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
MSC were negative for MHC 2 , CD 40 , CD40L , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , positive for MHC 1 , and kept this expression pattern during osteogenic differentiation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Disease improvement was paralleled by decreased expression of MHC class 2 on monocytes and B lymphocytes and reduced expression of CD 80 and CD 86 on B lymphocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In both cases , infiltration by APCs was accompanied by strong CD 80 and CD 86 expression and macrophage nitric oxide ( NO ) synthase up regulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Costimulatory molecules such as lymphocyte function associated antigen ( LFA ) 1 ( CD11a ) , LFA 3 ( CD 58 ) , intercellular adhesion molecule ( ICAM ) 1 ( CD 54 ) , neuronal cell adhesion molecule ( NCAM ) ( CD 56 ) , B 7 1 ( CD 80 ) , or B 7 2 ( CD 86 ) are important regulatory elements in healthy immunological cascades , but their role in acute myeloid leukemia ( AML ) has only been rarely investigated . ^^^ The surface expression of CD11a ( 27 of 29 cases positive with an average of 71 % positive blasts ; 27 ( + ) / 29 , 71 % ) , CD 54 ( 23 ( + ) / 33 , 37 % ) , CD 56 ( 24 ( + ) / 93 , 20 % ) , CD 58 ( 29 ( + ) / 29 , 95 % ) , CD 80 ( 13 ( + ) / 28 , 30 % ) , and CD 86 ( 19 ( + ) / 29 , 39 % ) was measured . ^^^ CD 80 and CD 86 expressions were similar in all FAB types . ^^^ Patients who had responded to AML CG therapy showed higher CD 80 proportions and lower CD 86 proportions compared to the `` nonresponder ' ' group . ^^^ Whereas cases with more than 15 % CD 80 ( + ) cells had a significantly lower probability for relapse free survival , only cases with more than 65 % CD 86 ( + ) were characterized by a significantly lower probability for relapse free survival . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
There are also features of activation observed in the splenic macrophages of mice consuming ethanol chronically , including increased up regulation of CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Activation also increased the proportion of OX62+ cells expressing CD 40 , CD 54 and CD 86 and their intensity of expression , however , unlike murine BMDCs , it had no effect on CD 80 and MHC class 2 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 40 , CD 80 and CD 86 ) at the T cell DC synapse . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
As an extracellular delivered protein , Vpr down modulated the expression of several immunologically important molecules including CD 40 , CD 80 , CD 83 and CD 86 costimulatory molecules on MDM ( monocyte derived macrophage ) and MDDC ( monocyte derived dendritic cells ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The aim of the study was to evaluate the function of monocytes in children with leukemias and lymphomas based on the expression of critical costimulatory , activatory and adhesion molecules ( CD 80 , CD 86 , HLA DR and CD 54 = ICAM 1 ) , estimated with tricolor flow cytometry . ^^^ In comparison to the control group we found a lower percentage of monocytes with costimulatory molecules ( CD 80 before and CD 86 after lipopolysaccharide stimulation ) at the time of diagnosis and of monocytes with HLA DR molecules after remission induction . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of HLA ABC , HLA DR , CD 40 , CD 80 , CD 83 and CD 86 was evaluated by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The role of costimulatory molecules CD 80 and CD 86 and IFNgamma in the pathogenesis of ulcerative colitis . ^^^ We quantified the expression of CD 80 , CD 86 , and IFNgamma in colonic mucosa of patients affected by ulcerative colitis and correlated it with clinical and biochemical parameters to identify the context of this up regulation . ^^^ CD 80 , CD 86 , and IFNgamma expression in the colonic mucosa was quantified using reverse transcription polymerase chain reaction . ^^^ CD 80 was detectable in seven patients , while CD 86 and IFNgamma expression was evident in all UC patients . ^^^ CD 80 and CD 86 were not detectable in control specimens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulated B cells ( sBc ) express high levels of MHC class 1 and 2 , as well as the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Currently , numerous preclinical studies have reported immunomodulatory cytokine gene therapy , such as interleukin 2 , interleukin 12 , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Consistent with the receptor expression , AMs showed no sensitivity to CpG ODN , whereas other macrophage populations secreted tumor necrosis factor alpha , interleukin 12 p 40 , and interleukin 6 , and enhanced expression of CD 40 , CD 80 , and CD 86 , in response to CpG ODN . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , levels of CD 80 and CD 86 on human monocytic THP 1 cells were decreased in a dose dependent manner in the presence of 0 . 1 to 10 microM DZ 2002 , and decreases were also seen in IL 12 and tumor necrosis factor alpha production from both mouse thioglycollate stimulated peritoneal macrophages and THP 1 cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS : Airway EOSs were recovered from ovalbumin sensitized and challenged BALB / c mice , these EOSs were then cocultured with CD4+ cells isolated from sensitized mice in the absence or presence of anti CD 80 or / and CD 86 monoclonal antibodies . ^^^ RESULTS : Airway EOSs functioned as CD 80 and CD 86 dependent antigen presenting cells to stimulate lung CD4+ lymphocytes to produce interleukin 4 , interleukin 5 and interleukin 13 , but not interferon gamma in in vitro assay . ^^^ When instilled intratracheally in sensitized recipient mice , airway EOSs primed lung Th 2 cells in vivo for interleukin 4 , interleukin 5 and interleukin 13 , but not interferon gamma , production during the in vitro culture that was also CD 80 and CD 86 dependent . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast to both nonleukemic B cell precursors and mature B cells , the response of precursor B ALL cells was characterized by increased CD 40 expression but only small changes in CD 86 levels and no induction of CD 80 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The percentages of blood DC expressing the cell surface marker CD1a , co stimulatory molecules CD 80 and CD 86 , adhesion molecule CD 54 , and chemokine receptors CCR 1 , CCR 2 , CCR 5 , and CXCR 4 were not affected in GBS or CIDP . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Exposure to proteoliposomes from serogroup B Neisseria meningitidis ( PL ) induced up regulation of MHC 2 , MHC 1 , CD 40 , CD 80 and CD 86 expression on the surface of murine bone marrow derived dendritic cells ( DC ) . ^^^ CD 40 , CD 80 and CD 86 were up regulated on bone marrow derived macrophages ( MPhi ) upon stimulation with PL . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using one CpG ODN , DSP 30 , we observed that it could upregulate not only Toll like receptor 9 ( TLR 9 ) mRNA expression in activated B cells , but also the early expression of CD 69 followed by the sequential expression of CD 80 , CD 86 and the nuclear factor ( NF ) kappaB pathway . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
While no diurnal rhythmicity in the expression of TLRs 1 , 2 , 4 or 9 was observed , the upregulation of costimulatory ( CD 80 and CD 86 ) and antigen presenting ( MHC class 2 ) molecules on CD 14 ( + ) monocytes following activation with specific TLR ligands was greater ( P < 0 . 05 ) in samples obtained in the evening compared with the morning . ^^^ Furthermore , the upregulation of CD 80 , CD 86 , MHC class 2 and interleukin 6 by CD 14 ( + ) monocytes following activation with specific TLR ligands was decreased ( P < 0 . 05 ) in samples obtained following exercise compared with at rest . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although CIITA ( / ) DC lack MHC class 2 , they maintained normal expression of costimulatory molecules CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Sections were stained with antibodies against : EG 2 ( eosinophils ) , CD 4 ( T cells ) , CD 68 ( macrophages ) , CD1a ( Langerhans cells ) , CD 28 ( on T cells ) and costimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Triptolide suppresses CD 80 and CD 86 expressions and IL 12 production in THP 1 cells . ^^^ RESULTS : Triptolide suppressed CD 80 and CD 86 expressions on IFN gamma ( 500 kU / L ) and LPS ( 1 mg / L ) activated THP 1 cells at nontoxic dosages of 2 . 5 0 . 625 microg / L . ^^^ CONCLUSION : Triptolide impairs the antigen presenting function by inhibiting CD 80 and CD 86 expressions and decreased IL 12p40 and IL 12p70 ( bioactive form ) productions from the activated THP 1 cells . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This study investigated how CD 80 and CD 86 regulated CD 152 expression in a low responding cardiac transplant model with CD 152 mediated long term graft acceptance . ^^^ Proliferation assays , quantitative ( Q ) real time polymerase chain reaction ( PCR ) , flow cytometric analyses , and fluorescence microscopy were conducted to examine the roles of CD 80 and CD 86 in CD 152 expression . ^^^ A 100 splenic T cells were hyporesponsive to donor specific stimulation , and anti CD 80 , anti CD 86 , or anti CD 152 treatment significantly enhanced the proliferation response of the B10 . ^^^ Proliferation assays and Q PCR revealed that CD 152 inhibited T cell proliferation and , at the same time , decreased CD 152 expression by secluding CD 80 and CD 86 from CD 28 engagement . ^^^ Besides , CD 152 engagement by CD 80 decreased CD 152 mRNA transcription , and CD 152 engagement by CD 86 inhibited surface expression of CD 152 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In particular , on the targeted APC , modulation of the expression of costimulatory molecules , CD 80 , CD 86 , CD 83 , and B7RP 1 , plays an important role in the effect of the ADP ribosylating CTA 1 based adjuvants on the development of tolerance or active IgA immunity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC 1 from CHB patients showed lower expression of costimulatory molecules CD 80 , CD 86 and impaired allostimulatory mixed lymphocyte reaction ( AMLR ) compared with those in normal controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Meanwhile , decreased expression of presentation molecules ( HLA DR and CD1a ) and costimulatory molecules ( CD 80 and CD 86 ) and down regulated IL 12 secretion were also observed . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After 2 more days , Mo DCs were harvested and their CD 80 , CD 86 , and CD 83 expression was assessed by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Markers of DC maturation ( CD 80 , CD 86 , HLA DR ) were upregulated on monocytes and macrophages ( P < . 05 ) , and discrete populations of mature DC were observed ( P < . 05 ) after CCRE inhalation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A ) There was differential expression of CD 69 , CD 80 and CD 86 exposure to sCD40L and LCD40L was ensued by the strongest % MFI changes over control . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The interaction of CD 28 with one of the B 7 molecules ( CD 80 and CD 86 ) on professional antigen presenting cells ( APC ) is generally considered to be the most important co stimulatory signal for T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometry analysis revealed that the addition of saliva to bone marrow cells inhibits the differentiation of DC and decreased the population of differentiated immature DC , increasing the levels of major histocompatibility complex ( MHC ) class 2 while not altering the expression of costimulatory ( CD 40 , CD 80 and CD 86 ) and adhesion ( CD 54 ) molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Addition of JM 34 during DC maturation inhibited the expression of some maturation markers : specifically , MHC molecule up regulation was totally inhibited and CD 83 expression was significantly reduced , while up regulation of CD 86 , CD 80 or CD 40 was less affected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The best characterized pathway includes CD 28 , its homologue CTLA 4 and their ligands CD 80 and CD 86 . ^^^ Two amino acid substitutions ( L104E and A29Y ) gave rise to slower dissociation rates for both CD 86 and CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Microglia like cells expressed MHC class 1 , class 2 , CD 40 , CD 80 , CD 86 , and IFN gammaR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Lactobacillus exposed MDCs up regulated HLA DR , CD 83 , CD 40 , CD 80 , and CD 86 and secreted high levels of IL 12 and IL 18 , but not IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , binding of the B 7 family members CD 80 or CD 86 on professional antigen presenting cells to CD 28 on T cells is considered to provide an important costimulatory signal . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The most important costimulatory protein is the monovalent homodimer CD 28 , which interacts with CD 80 and CD 86 expressed on antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We evaluated the effect of dexamethasone on the expression and regulation of MPhi B 7 family receptors ( B 7 1 , CD 80 ; B 7 2 , CD 86 ) and on their ability to co stimulate T cells . ^^^ CBMPhi were less able to up regulate CD 80 and CD 86 than PBMPhi ( p < 0 . 05 ) . ^^^ Dexamethasone inhibited the up regulation of CD 80 , CD 86 , and HLA DR on PBMPhi and even more so on CBMPhi ( p < 0 . 05 versus PBMPhi for CD 80 and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 7 treatment of naive CD 4 ( + ) T cells induced their ability to prime monocytes , as was indicated by induction of CD 80 and CD 86 expression on monocytes cocultured with IL 7 treated naive CD 4 ( + ) T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In support of this hypothesis it has been shown that these cells appear to contain cytoplasmic stores of molecules required for this function , i . e . major histocompatibility complex class 2 ( DR ) antigen , CD 80 and CD 86 . ^^^ Cross linking of the Mac 1 molecule ( CD 18 + CD11b ) was shown to result in rapid cell surface expression of CD 80 , CD 86 and DR antigen on the surface of normal human peripheral blood neutrophils . ^^^ The level of expression of CD 80 , CD 86 and DR antigen on these large cells was comparable to , and in some cases greater than , the levels found expressed on the surface of monocytes obtained from the same donors . ^^^ Following rapid translocation onto the cell surface , CD 80 and CD 86 appeared to be colocalized within large clusters reminiscent of the supramolecular antigen clusters previously found on conventional antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results show that IFN gamma , but not IL 1beta , augmented the surface expression of CD 80 , CD 83 and CD 86 molecules without inducing IL 12 production from DCs . ^^^ When combined , IFN gamma + IL 1beta treatment profoundly up regulated the expression of CD 80 , CD 83 , CD 86 and major histocompatibility complex ( MHC ) class 2 antigens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells expressed high levels of HLA DR , CD 13 , CD 33 , and CD 86 , and low levels of CD 40 , CD 80 , CD 83 , CD 123 , CD 209 , TLR 2 and TLR 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC exposed to live EBs acquired a mature DC morphology ; expressed high levels of major histocompatibility complex ( MHC ) class 2 , CD 80 , CD 86 , CD 40 , and ICAM 1 ; produced elevated amounts of interleukin 12 and tumor necrosis factor alpha ; and were efficiently recognized by Chlamydia specific CD4+ T cells . ^^^ In contrast , UV EB pulsed DC expressed low levels of CD 40 and CD 86 but displayed high levels of MHC class 2 , ICAM 1 , and CD 80 ; secreted low levels of proinflammatory cytokines ; and exhibited reduced recognition by Chlamydia specific CD4+ T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , we demonstrate that the expression of human leukocyte antigen ( HLA ) class 1 and 2 molecules , but not the co stimulatory proteins CD 40 , CD 80 and CD 86 , substantially increase during expansion of neurospheres . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Specifically , the effects of 17beta estradiol on basal and LPS induced surface staining of Class 1 and 2 MHC , as well as CD 40 , CD 80 , CD 86 , CD 152 , CD 28 , CD 8 , CD11b , Fas , FasL , and also ERalpha and ERbeta , were examined in N 9 microglial cells . ^^^ However , CD 8 , CD 86 , CD11b , and CD 28 were unaffected by estrogen , and CD 80 cell surface staining significantly increased following estrogen exposure . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A representative cell line , HB 2 , that expressed DC markers including CD 83 , CD 80 and CD 86 could be induced to produce IL 12 through CD 40 stimulation . ^^^ There was no change in expression of major histocompatibility complex class 1 and class 2 antigens , CD 83 , CD 40 , CD 4 , CD11c , CD 80 , CD 86 , CD 54 , and CD 58 , or IL 12 production in the HIV infected HB 2 cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , when analyzing DC , we found that up regulation of CD 80 , CD 86 , CD 40 , RANK and PDL 1 occurred only when peptide was co administered with LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Other monocyte surface molecules ( CD 40 , CD 80 , CD 86 , MHC class 2 ) were also determined to test the specificity of the effect on FcgammaR expression levels . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The authors report in this article that purified Tax induces DC activation involving an increase in the production of CD 80 and CD 86 mRNA in the absence of corresponding protein synthesis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Exposure of peripheral blood mononuclear cells to CpG ODN 2216 led to decreased expression of the monocyte marker CD 14 and increased expression of the dendritic cell marker CD 83 , as well as increased expression of HLA DR , CD 40 , CD 80 , and CD 86 among the monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our objectives in this study were , therefore , to determine if the expression of co stimulatory molecules , such as CD 40 , CD 80 , or CD 86 , on peritoneal / splenic / liver macrophages were altered by sepsis ( cecal ligation [ CL ] and puncture [ CLP ] or necrotic tissue injury ( CL ) alone ; and to establish the contribution of such changes to the response to septic challenge using mice that are deficient in these receptors . ^^^ The macrophages were then analyzed by flow cytometry for their ex vivo expression of CD 40 , CD 80 , CD 86 , and / or MHC 2 . ^^^ RESULTS : The expression of CD 86 and MHC 2 , but not CD 40 or CD 80 , were significantly decreased on peritoneal macrophages after the onset of sepsis or CL alone . ^^^ Alternatively , splenic macrophages from septic or CL mice did not show changes in the expression of CD 80 , CD 86 , or CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Critical contribution of CD 80 and CD 86 to induction of anterior chamber associated immune deviation . ^^^ Expression of CD 80 , CD 86 , OX 40 ligand ( OX40L ) and CD 70 was analyzed by flow cytometry . ^^^ Contribution of CD 80 and CD 86 to induction of ACAID was determined by the administration of blocking antibodies at intraocular antigen inoculation in vivo . ^^^ TGF beta treated PEC expressed CD 80 and CD 86 but not OX40L or CD 70 . ^^^ Antigen stimulated T cells proliferated and produced IL 10 , but not IFN gamma , in response to co stimulation by TGF beta treated PEC , which was abrogated by blocking antibodies against CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The intrinsic cellular activities of the lipopeptide analogues incorporated into liposomes were monitored , in vitro , by measuring the up regulation of the cell surface markers CD 80 , CD 83 , CD 86 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
No significant changes in percentages of CD 86 and CD 80 APCs or CD4+ , CD25+ T cells were documented . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , monocytes that were precultured with CD4+CD25+ Tregs displayed limited upregulation of human leukocyte antigen class 2 , CD 40 and CD 80 , and downregulation of CD 86 compared with control treated monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
BACKGROUND : The surface expression of T cell costimulatory molecules CTLA 4 and CD 28 and their counter ligands , B 7 molecules ( CD 80 , CD 86 ) , is differentially induced for T cell activation and expansion in allergic asthma . ^^^ METHODS : Plasma concentrations of soluble CTLA 4 ( sCTLA 4 ) , CD 28 , CD 80 and CD 86 in 51 children with chronic allergic asthma with or without inhaled corticosteroid treatment , and 22 sex and age matched control subjects were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This review will discuss the mechanisms of suppression , which include the local secretion of cytokines such as TGF beta and direct cell contact through binding of cell surface molecules such as CTLA 4 on suppressor T cells to CD 80 and CD 86 molecules on effector T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The following parameters were studied : surface molecules expression ( CD 18 , CD11b , CD 44 , CD 162 , CD15s , CD 80 , CD 86 , CD 16 , CD49d , CD 29 , CD 25 , HLA DR , Toll like receptor 4 [ TLR 4 ] , CXCR 1 , CCR 2 , CCR 5 , CX3CR1 ) , oxidative burst response , monocyte platelet conjugates ( using antibodies against CD 45 , CD 14 , CD41a ) , and platelet activation ( CD62P , PAC 1 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Also , the four culture conditions generated CD11c+ DC with comparable levels of major histocompatibility complex ( MHC ) class 2 , CD 40 , CD 80 and CD 86 expression , with the exception of cells cultured under serum free conditions in the absence of IL 4 , which displayed suboptimal levels of these markers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression and functional roles of costimulatory receptors , CD 28 and cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , and their ligands , CD 80 and CD 86 , on primary mouse CD 4 ( + ) T cells after activation with different doses of Con A were studied . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression levels of CD1a , CD 80 , CD 83 , and CD 86 as expressed by mean fluorescence intensity ( MFI ) on DCs differentiated from immature DCs after culture with 1 muM of piceatannol for 2 days were enhanced and decreased endocytic activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using flow cytometry , the surface expression of receptors implicated in phagocytosis ( CD 44 , CD 36 , CD 51 , CD 61 , CD 14 ) , antigen presenting capacity ( human leukocyte antigen ( HLA ) DR ) , costimulatory molecules ( CD 80 , CD 86 , CD 40 ) and complement receptor type 3 were assessed in AM from 18 patients with COPD , 14 smokers with normal lung function and nine nonsmokers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , we show that , in addition to NK cells , CD34 / fibroblast co cultures also yield myeloid CD1a ( + ) CD 38 ( + ) CD 68 ( + ) CD 86 ( + ) HLA DR ( + ) CD 14 ( ) CD 80 ( ) dendritic cells ( DCs ) after 3 4 weeks in culture . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 3 DCs had similar morphology as plasma cells , but a different immune phenotype , namely , CD 80 ( ) , CD 86 ( ) , CD 14 ( ) , and MHC class 2 ( + ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , this was not due to an immaturity , since MDC from hepatic LN had significantly higher expressions of HLA DR , CD 80 , and CD 86 compared to MDC from inguinal LN . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 , but not CD 86 , express on cultured murine keratinocyte stem cells . ^^^ Immunostaining showed that over a 7 day culture period , KSCs expressed increasing amounts of CD 80 , while CD 86 could not be detected . ^^^ This study demonstrates that the costimulatory molecule CD 80 , but not CD 86 , may be expressed on cultured KSCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show here that the uptake of chitosan at inductive mucosal sites involves CD11b / c+ APC and that chitosan feeding increases the percentage of OX62+ dendritic cells , which up regulate the major histocompatibility complex class 2 antigens without changing the expression of costimulatory CD 80 or CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Blockade of CD 80 and CD 86 at the time of implantation inhibits maternal rejection to the allogeneic fetus in abortion prone matings . ^^^ CD28 / CTLA 4 interactions with their specific B 7 ligands ( CD 80 and CD 86 ) play a decisive role in antigenic and allogenic responses . ^^^ In the present study , abortion prone CBA / J females mated with DBA / 2 males were administered with anti CD 80 and anti CD 86 monoclonal antibodies ( mAbs ) on day 4 of gestation ( time of murine implantation ) . ^^^ We demonstrated that the combined use of anti CD 80 and anti CD 86 mAbs induced maternal tolerance to the fetus in the abortion prone CBA / J mice , and displayed expansion of the maternal CD 4 ( + ) CD25+ regulatory T cell population and up regulated expression of CTLA 4 , suggesting an active mechanism of regulatory T cells in suppressing maternal rejection to the fetus . ^^^ Together , these findings indicated that blocking CD 80 and CD 86 enhanced maternal tolerance to her fetus in mice by increasing regulatory T cell function and skewing toward a Th 2 response . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recently , we and others have demonstrated , in vitro and in vivo , that coexpression of CD 80 and CD 86 costimulatory molecules enhances the immunogenic capacity of a recombinant vaccinia virus ( rVV ) encoding different tumor associated antigens . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CTLA 4 is an essential protein in the regulation of T cell responses that interacts with two ligands found on the surface of APCs ( CD 80 and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
IL 10 deficiency caused early maturation and activation of pulsed DC ( i . e . , high CD11c , CD 40 , CD 80 , CD 83 , CD 86 , IL 1 , IL 12 , and the T cell attracting chemokine CCL27 / CTACK ) and consequently an enhanced ability to process and present Ags for a rapid and robust T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Analysis of CD11c ( hi ) dendritic cells ( DC ) reveals that infection with live L . monocytogenes induces higher levels of CD 40 , CD 80 and CD 86 expression than immunization with HKLM . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The secretion of CyC ceased , consistent with the peak of the surface expression of phenotypic markers ( CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , and major histocompatibility complex class 2 ) , characteristic for the mature DC stage , whereas the secretion of cathepsins did not correlate with the maturation stage . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Expression of HLA ABC , HLA DR , CD 54 , CD 40 , CD 58 , CD 80 , and CD 86 by peripheral blood ( PB ) monocytes and PF macrophages was quantitated as mean fluorescence intensities by flow cytometry . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cytokines as well as cellular surface molecule such as IL 12 , TNF alpha , CD 1 alpha , CD 83 , MHCII , CD 80 and CD 86 were detected . ^^^ CD 1 alpha , CD 83 , MHCII , CD 80 and CD 86 was high expressed in survivin DC cells , however , it was low expressed in blank DC cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although previous studies have shown that altered B 7 costimulation plays a critical role in UV irradiation induced regulation of immunity , the individual roles of the B 7 receptors ( CD 28 and CTLA 4 ) or the B 7 family members ( CD 80 and CD 86 ) have not been explored . ^^^ Next , we used mice deficient for CD 80 , CD 86 , or both in photocarcinogenesis studies to assess the relative contributions of these CTLA 4 ligands . ^^^ Double deficient mice showed significantly reduced UV induced skin tumor development , whereas CD 86 ( / ) mice produced skin cancer earlier compared with CD 80 ( / ) and control mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The results presented in this study demonstrate that the saturated fatty acid , lauric acid , up regulates the expression of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) , MHC class 2 , and cytokines ( IL 12p70 and IL 6 ) in bone marrow derived DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After spore infection , immature DCs displayed a mature phenotype ( CD 83 ( bright ) , HLA DR ( bright ) , CD 80 ( bright ) , CD 86 ( bright ) , CD 40 ( bright ) ) and enhanced costimulatory activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Infectious HSV 2 induced apoptotic death , decreased expression of HLA DR , CD 40 , CD 80 , CD 83 , and CD 86 , and increased release of interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) ( CCL 3 ) , and RANTES ( regulated on activation normal T cells expressed and secreted ) ( CCL 5 ) but not IL 12 or interferon alpha ( IFN alpha ) by macaque DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : The expression rates of CD 80 , CD 86 , CD 40 , and HLA DR in the mDCs pulsed with HBcAg were 55 % + / 26 % , 80 % + / 13 % , 70 % + / 13 % and 73 % + / 24 % respectively , significantly higher than those of the control group ( 29 % + / 25 % , 35 % + / 18 % , 44 % + / 26 % and 45 % + / 23 % respectively , all P < 0 . 05 ) . ^^^ CONCLUSION : Pulsation of mDCs in vitro by HBcAg or HBsAg enhance the expression of CD 80 , CD 86 , CD 40 , and HLA DR , and increase the ability of mDCs to stimulate the proliferation of autologous T lymphocytes . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The mRNA expression of CD 80 , CD 86 , IRAK 3 , and CCL 2 was successfully detected in all of the tissues and cell lines except for Hep G 2 cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After poly ( 1 : C ) maturation , both DC types display a marked upregulation of CD 80 , CD 83 and CD 86 and the same pattern of gene expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Two tumor cell supernatants , MDA 468 and 293T , were found to be able to affect the in vitro differentiation of DCs from monocytic precursors , leading to the generation of a distinct type of DC with markedly reduced expression of DC SIGN , downregulation of CD11c , HLA DR and CD1a , and upregulation of CD 123 , HLA ABC , CD 80 , CD 40 , CD 86 , CD 54 , CD 83 , CD 25 and CCR 7 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Phenotypic characterization of MSCs by flow cytometry showed expression of MHC Class 1 alloantigens , but minimal expression of Class 2 alloantigens and costimulatory molecules , including CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Aberrant production of soluble costimulatory molecules CTLA 4 , CD 28 , CD 80 and CD 86 in patients with systemic lupus erythematosus . ^^^ OBJECTIVE : The costimulatory interactions of the B 7 family molecules CD 80 and CD 86 on antigen presenting cells with their T cell counter receptors CD 28 and CTLA 4 modulate T lymphocyte mediated immune responses in a reciprocal manner . ^^^ We investigated the possible aberrant production of soluble ( s ) forms of the T cell costimulatory molecules CD 80 , CD 86 , CD 28 and CTLA 4 in plasma of patients with systemic lupus erythematosus ( SLE ) , an autoimmune disease arising from T lymphocyte dysregulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results showed that RPMI with TCH yielded the highest percentage of cells expressing both CD 80 and CD 86 molecules and , in contrast to other media , the higher percentage of CD86+ cells in comparison to CD80+ cells . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A differential pattern in co stimulatory molecule expression is seen ; CD 80 is unchanged but CD 86 is markedly elevated and such a change is not seen in the alternatively activated macrophage but has been previously reported in monocytes resident in the non inflamed gut . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It was previously thought that keratinocytes did not express the CD 80 and CD 86 which provide the most important costimulatory signals for the antigen specific T cell activation . ^^^ In this study , neonatal murine keratinocytes were cultured for 7 days , the results of flow cytometry and confocal microscopy showed that CD 80 could be detected on keratinocytes , while CD 86 could not be detected all the time . ^^^ The expression level of the CD 80 mRNA amplified significantly from day 1 to day 7 , as expression of the control beta actin , but CD 86 was not detected . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
As ICAM 1 is expressed on virtually any cell , it becomes a crucial molecule for the activation of CD 8 ( + ) T cells in the absence of co stimulation provided by CD 80 and CD 86 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The DCs expressed CD11c , CD11b , and the costimulatory molecules CD 40 , CD 80 and CD 86 , characteristic of mature DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the normal adult CNS , microglial cells exist in a resting state characterized by a minimal or negative expression of MHC class 2 and the co stimulatory molecules CD 80 , CD 86 and CD 40 and exhibit a unique ramified morphology . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
As compared with soluble rPA , it was found that coculture of DC with rPA loaded microparticles stimulated higher levels of MHC 2 , CD 54 , CD 80 and CD 86 expression ( p < 0 . 05 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Following incubation with CpG oligonucleotide , Namalwa cells secreted increased amounts of TNF , IL 6 , and IL 10 and expressed the costimulator molecules CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The aim of this study was to determine the expression of MHC class 2 and the co stimulatory molecules CD 40 , CD 80 and CD 86 in keratinocytes derived from healthy oral mucosa and oral carcinomas . ^^^ All keratinocyte lines expressed CD 40 constitutively ; by contrast , CD 80 and CD 86 were universally absent . ^^^ Loss of CD 80 and CD 86 may be one means whereby tumours escape immunological surveillance . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , we did not observe increased expression of maturation associated and functionally relevant surface antigens ( CD 14 , HLA DR , CD 40 , CD 83 , CD 80 and CD 86 ) , significant secretion of IL 12 and IFN alpha in culture supernatant , or enhanced antitumour activation of cytokine induced killer cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Activated B cells exhibited increased expression of activation markers and ligands that are critical for cross talk with T cells ( CD 19 , CD 25 , CD 80 , CD 86 , MHC 1 , MHC 2 , and CD 40 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
LC from CD40L KO mice expressed similar levels of surface molecules such as CD 40 , CD 80 , CD 86 , and MHC class 2 , compared with those from wild type mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
For example , LPS induced up regulation of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) is abrogated in antigen presenting cells ( APC ) deficient in TRIF or TRAM , two adaptors that are responsible for TLR 4 mediated production of Type 1 IFN . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The CB DCs treated with Bryo 1+CI exhibited morphologic characteristics of mature DCs , expressed increased levels of CD1a , CD 80 , CD 83 , CD 86 , and MHC class 2 as well as enhanced ability to induce proliferation of alloreactive T cells isolated from cord blood and IFN gamma production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The activation of BMDC was characterized by studying expression of cell surface co stimulatory molecules ( CD11c , MHC class 2 , CD 40 , CD 80 , and CD 86 ) , expression of genes encoding Toll like receptors and release of IL 12 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Optimal activation of T cells requires that dendritic cells undergo a process of maturation resulting in the increased expression of costimulatory molecules , such as CD 40 , CD 86 , and CD 80 , and the production of cytokines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Intriguingly , ELD 1 cells stimulated interferon ( IFN ) gamma production by purified allogeneic CD8+ T cells in an IL 2 but not IL 12 dependent manner , but failed to stimulate CD4+ T cells due to their lack of HLA DR , CD 40 , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The adaptive immune response is dependent upon upregulation of costimulatory molecules ( UCM ) such as CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we demonstrated that WKYMVm enhanced the surface expression of CD 80 , but not that of CD 40 , CD 86 and MHC class 2 , on mouse bone marrow derived dendritic cells which is one of the essential costimulatory signals for the induction of immune responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment of DC with PS G resulted in the enhanced cell surface expression of CD 80 , CD 86 , CD 83 , CD 40 , CD 54 , and human leukocyte antigen ( HLA ) DR , as well as the enhanced production of interleukin ( IL ) 12p70 , p 40 , and IL 10 and also IL 12p35 , p 40 , and IL 10 mRNA expression , and the capacity for endocytosis was suppressed in DC . ^^^ Further , inhibition of NF kappaB by helenalin and p 38 MAPK by SB 98059 prevented the effects of PS G in the expression of CD 80 , CD 86 , CD 83 , CD 40 , CD 54 , and HLA DR and production of IL 12p70 , p 40 , and IL 10 in various degrees . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Leukemic B cells up regulated expression of CD 54 , CD 80 , CD 86 , and CD 95 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Down regulation of costimulatory ligands CD 80 and CD 86 on synovial fibroblasts was identified as one mechanism of immunosuppression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Splenic CD11c ( + ) dendritic cells ( DC ) from mice infected with malaria alone and coinfected mice showed similarly increased expression of CD 40 , CD 80 , and CD 86 , but DC from coinfected mice were unable to induce CD 4 ( + ) T cell proliferation and optimal IFN gamma production in response to the malaria antigen in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment of cells from CTLA 4 expressing tumor lines with recombinant forms of the CTLA 4 ligands CD 80 and CD 86 induced apoptosis associated with sequential activation of caspase 8 and caspase 3 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This secreted HMGB 1 is necessary for the up regulation of CD 80 , CD 83 , and CD 86 surface markers of human dendritic cells and for IL 12 production . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Checking for a possible influence on LPS induced dendritic cell maturation , we found that tetrahydro 4 aminobiopterin down regulated MHC class 2 expression and counteracted LPS induced down regulation of ICOS ligand , while expression of CD 40 , CD 86 , CD 80 , B 7 H1 , and B 7 DC remained unchanged . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Injection of R 848 and CpG oligodeoxynucleotides alone enhanced the innate immune responses in vivo as demonstrated by high serum levels of inflammatory cytokines , including IL 12p70 and IFN alpha , and increased expression of CD 80 , CD 86 , and CD 40 on CD11c ( + ) dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Exposure of a LC like cell line to ATPgammaS in the presence of LPS and GM CSF augmented the induction of 1 A , CD 80 , CD 86 , IL 1beta , and IL 12 p 40 while inhibiting the expression of IL 10 , suggesting that the immunostimulatory activities of purinergic agonists in the skin are mediated at least in part by P2Rs on APCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Notably , the NKT : NK and CD 80 : CD 86 ratios were correlated with the IL 4 : IFN gamma ratio , indicative of multiple converging modes of regulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Curcumin significantly suppressed CD 80 , CD 86 , and MHC class 2 expression , but not MHC class 1 expression , in the DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Polymorphisms of B 7 ( CD 80 and CD 86 ) genes do not affect disease susceptibility to sarcoidosis . ^^^ BACKGROUND : B 7 proteins ( CD 80 , CD 86 ) are costimulatory molecules expressed on antigen presenting cells and are essential factors for T cell activation . ^^^ METHODS : Gene polymorphisms located in CD 80 promoter , CD 80 exon 3 and exon 8 of CD 86 were examined in 146 Japanese sarcoidosis patients and 157 healthy controls using single strand conformation polymorphism and direct sequencing . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 4 ( + ) cells were cocultured with M 1 cell ( human fibroblast ) transfectants expressing HLA DR 1 , CD 80 and CD 86 alone or in combination . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Glomerular expression of CD 80 and CD 86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis . ^^^ The participation of renal expression of CD 80 and CD 86 in the immunopathogenesis of crescentic Th 1 mediated anti glomerular basement membrane ( anti GBM ) glomerulonephritis ( GN ) has not been assessed . ^^^ Immunohistochemical staining demonstrated prominent upregulation of both molecules in glomeruli of mice with anti GBM GN , suggesting a potential role for the local expression of CD 80 and CD 86 in nephritogenic effector T cell responses . ^^^ For testing this hypothesis , control or inhibitory anti CD 80 and / or anti CD 86 mAb were administered to mice during the effector phase of the disease but after the establishment of a systemic immune response . ^^^ Anti CD 80 or anti CD 86 mAb treatment had no effect on the development of GN or infiltration of leukocytes into glomeruli ; however , administration of anti CD80 / 86 mAb attenuated glomerular accumulation of CD4+ T cells and macrophages , crescent formation , and proteinuria , correlating with reduced antigen specific skin delayed type hypersensitivity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our data show that both triptolide and Dex prevented the differentiation in immature MoDC by inhibiting CD1a , CD 40 , CD 80 , CD 86 and HLA DR expression but upregulating CD 14 expression , as well as by reducing the capacity of MoDC to stimulate lymphocyte proliferation in the allogeneic mixed lymphocyte reaction . ^^^ They blocked the maturation of MoDC as totally blocked induction of CD 83 expression and absent upregulation of CD 40 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Vpr impaired the expression of CD 80 , CD 83 , and CD 86 at the transcriptional level without altering normal cellular transcription . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The co stimulatory interactions of the B 7 family molecules CD 80 and CD 86 on antigen presenting cells , together with their T cell counter receptors CD 28 and cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) , modulate T lymphocyte mediated immune responses in a reciprocal manner . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
There are currently seven known members of the family : B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , inducible costimulator ligand ( ICOS L ) , programmed death 1 ligand ( PD L 1 ) , programmed death 2 ligand ( PD L 2 ) , B 7 H3 , and B 7 H4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , both gangliosides suppressed expression of CD 40 , CD 80 , CD 86 and major histocompatibility complex class 2 on DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
During differentiation into mature DCs , the cells exhibit de novo cell surface expression of CD 83 , CD 80 , CD 86 , CD 40 , CD 206 , CD 209 , CD120a , CD120b , and intracellular synthesis of IL 10 , increase their endocytotic capacity , and acquire characteristic stellate morphology . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We found that gp 96 peptide complexes derived from HCC cells induced the maturation of DCs by enhancing expression of human leukocyte antigen class 2 , CD 80 , CD 86 , CD 40 , and CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This finding correlated with the downregulation of CD 80 , CD 86 , and CD 54 expression and the increased proportion of plasmacytoid MHC class 2 ( + ) His 24 ( + ) His 48 ( + ) DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We analyzed the co stimulatory signals , CD 80 and CD 86 on tonsillar B lymphocytes . ^^^ The expression rates of CD 80 and CD 86 in the AT group with TI > or =8 were significantly decreased compared to those with TI was less than 8 ( TI < 8 ) , as well as with those in control ( tonsillar hypertrophy ) group . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In cellular activation , the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are thought to play nearly identical roles in T cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Whereas a large proportion of Langerhans cells expressed CD 86 in the steady state , CD 40 , and CD 80 were found on a smaller percentage . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Strikingly , IFN beta treatment reduced the expression of CD 40 , CD 54 , CD 80 , and CD 86 on LC , whereas IFN beta treated splenic DC showed enhanced expression of these molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Simultaneously , GGTI 298 induces the expression of CD 80 and CD 86 costimulatory molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : House dust extract activated bone marrow derived dendritic cells were found to produce IL 6 and IL 12 in a concentration dependent manner and to increase their expression of CD 40 , CD 80 , CD 86 , and MHC class 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , we demonstrate that statins reduce cell surface expression of other immunoregulatory molecules , which include MHC 1 , CD 3 , CD 4 , CD 8 , CD 28 , CD 40 , CD 80 , CD 86 , and CD 54 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results showed that StxB 1 and mStx 1 , but not native Stx 1 ( nStx 1 ) , resulted in enhanced expression of CD 86 , CD 40 , and major histocompatibility complex ( MHC ) class 2 molecules and , to some extent , also enhanced the expression of CD 80 on bone marrow derived DCs . ^^^ When mice were given StxB 1 subcutaneously , the levels of CD 80 , CD 86 , and CD 40 , as well as MHC class 2 expression by splenic DCs , were enhanced . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we demonstrate that oxidized phospholipids ( ox PLs ) , which are generated during infections , apoptosis , and tissue damage , interfere with DC activation , preventing their maturation . ox PLs blocked TLR 3 and TLR 4 mediated induction of the costimulatory molecules CD 40 , CD 80 , CD 83 , and CD 86 , the cytokines IL 12 and TNF , as well as lymphocyte stimulatory capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Bone marrow cells cultured in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 4 , and Flt 3 ligand ( FL ) produced immature DCs that expressed intermediate levels of major histocompatibility complex ( MHC ) class 2 , low levels of CD 80 and CD 86 molecules and displayed a high capacity of endocytosis . ^^^ Bone marrow derived DCs ( BMDCs ) matured in the presence of lipopolysaccharide ( LPS ) upregulated expression of MHC class 2 , CD 80 and CD 86 , while their phagocytic capacity was dramatically reduced . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Most lung DC expressed CD11c , CD11b , and low levels of MHC class 2 , CD 40 , CD 80 , and CD 86 , consistent with an immature myeloid phenotype . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Recent crystallographic reports have suggested that it may form lattice like arrays on the cell surface upon binding B7 . 1 / B7 . 2 ( CD 80 , CD 86 ) molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We observed that treatment of DC with HRV 14 ( R DC ) , a member of the major group HRV family , diminished their T cell stimulatory capacity and induced a promiscuous and deep anergic state in cocultured T cells despite high levels of MHC molecules as well as costimulatory molecules , e . g . , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , and independent of inhibitory soluble factors such as IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Freshly isolated LP DC expressed variable but generally low levels of CD 40 , CD 80 and CD 86 , which were up regulated by activation with LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 costimulatory molecules regulate crescentic glomerulonephritis by different mechanisms . ^^^ BACKGROUND : CD 80 and CD 86 costimulatory molecules have been shown to affect the induction of Th 1 mediated crescentic antiglomerular basement membrane ( GBM ) antibody initiated glomerulonephritis ( GN ) . ^^^ The aim of the current studies was to define the mechanisms by which CD 80 and CD 86 regulate the development of this disease . ^^^ METHODS : Anti GBM GN was induced in CD 80 / , CD 86 / , and CD80 / 86 / mice , as well as in C57BL / 6 controls . ^^^ To examine whether costimulation by OX 40 ligand compensates for the absence of CD 80 and CD 86 in inducing GN , OX 40 ligand was blocked in wild type and CD80 / 86 / mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
WSPG significantly enhanced the expression of co stimulatory molecules ( CD 80 and CD 86 ) and major histocompatibility complex ( MHC ) 2 , as did LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : The immunophenotypic analysis showed that in comparison with those in the control group and the LPS group the expressions of the costimulatory molecules , Ia ( d ) , CD 80 , and CD 86 , of the DCPs were significantly weaker in the MMF group and MMP + LPS group . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We observed that DCs undergo full maturation in response to Salmonella infection , as indicated by up regulation of cell surface marker proteins CD 80 , CD 83 , CD 86 , and human leukocyte antigen class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
On encounter with MUC 1 , similar to the encounter with LPS , immature DCs increase cell surface expression of CD 80 , CD 86 , CD 40 , and CD 83 molecules and the production of IL 6 and TNF alpha cytokines but fail to make IL 12 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In response to CD 40 ligand and tumor necrosis factor alpha , the cells acquire a mature phenotype of dendritic cells that is characterized by up regulation of human leukocyte antigen ( HLA ) ABC , HLA DR , CD 80 , CD 86 , CD 40 , and CD 54 and appearance of CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Concurrent to low GTP levels , a dose dependent reduction in the expression of CD 80 , CD 86 , CD 40 , CD 54 and CD 83 was seen which was accompanied by a decreased capacity of DC to stimulate T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , we report that HIFU treatment can cause the release of endogenous danger signals ( ATP and hsp 60 ) and exposure of dendritic cells ( DCs ) and macrophages to the supernatants of HIFU treated tumor cells leads to an increased expression of co stimulatory molecules ( CD 80 and CD 86 ) with enhanced secretion of IL 12 by the DCs and elevated secretion of TNF alpha by the macrophages . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Pollen up regulated the expression of CD 54 , CD 80 , CD 83 , CD 86 , HLA DR , CCR 7 , and CD 40 on DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although SERM exposed DC exhibited increased ability to internalize Ags , they were hyporesponsive to bacterial LPS : relative to control DC , they less efficiently up regulated the expression of MHC class 2 , CD 86 , and to a lesser extent , CD 80 and CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Conditioning of DCs with CSE also suppressed LPS mediated induction of CD 40 , CD 80 , and CD 86 , and suppressed maturation associated CCR 7 expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Transfection of CD 154 was functional in that it led to upregulated expression of CD 80 , CD 86 , ICAM 1 and MHC class 2 ( HLA DR ) on the B CLL cells and induction of allogeneic immune responses in MLR assays . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Compared with control transduced DC ( Ctrl DC ) , Dex DC expressed lower CD 40 , CD 80 and CD 86 but equivalent human leucocyte antigen DR . ^^^ Nevertheless , upon stimulation of lipopolysaccharide ( LPS ) or CD 40 ligand , the expression of CD 40 , CD 80 , CD 83 and CD 86 was upregulated on Ctrl DC but not on Dex DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Oxidized beta 2 GPI caused DCs to mature so that CD 83 appeared and CD 80 , CD 86 , human leukocyte antigen D region related ( HLA DR ) , and CD 40 increased . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
METHODS AND RESULTS : AGE BSA induced DCs maturation accompanied with increased expressions of CD1a , CD 40 , CD 80 , CD 83 , CD 86 , and MHC class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Then , we tested MUTZ 3 cells against a wider panel of sensitizers and irritants on a broader spectrum of cell surface markers ( HLA DR , CD 40 , CD 54 , CD 80 , CD 86 , B 7 H1 , B 7 H2 , B 7 DC ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , incubation of DC for 48 h with ESA did not modify the expression of costimulation or maturation markers ( CD 83 , CD 40 , CD 80 , CD 86 or HLA DR ) , but induced a decrease in CCR 6 expression associated with an increased expression of CCR 7 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Neuroblastoma cells transiently transfected to simultaneously express the co stimulatory molecules CD 54 , CD 80 , CD 86 , and CD137L generate antitumor immunity in mice . ^^^ AGN2a cells nucleofected to express the co stimulatory molecules CD 80 and CD 86 expressed higher levels of these molecules than cells that had been permanently transfected with these same plasmid vectors , and the nucleofected cells were as effective as the permanently transfected cells at inducing an antitumor response in vivo in a tumor prevention model . ^^^ AGN2a cells nucleofected with four separate plasmid vectors encoding CD 54 , CD 80 , CD 86 , and CD137L induced a T cell immune response in vitro and served as a potent tumor vaccine in the tumor prevention model . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Regardless of the maturation cocktail used , all DCs possessed the characteristic phenotype of mature , migratory DCs ( high expression of CD 40 , CD 80 , CD 83 , CD 86 , CCR 7 , MHC class 1 and MHC class 2 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , B CLL DCs generated from the 2 CLL subgroups up regulated MHC 2 , CD 80 , CD 86 , CD 83 , CD 40 , and CD 54 and down regulated CD 206 in response to stimulation with a cocktail of cytokines ( CyC ) and secreted increased levels of tumor necrosis factor alpha , interleukin ( IL ) 8 , IL 6 , IL 12 ( p 70 ) , and RANTES in a manner typical of mature normal DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
SIgA also triggers migration of DC to the T cell rich regions of PP , and regulates expression of CD 80 and CD 86 on DC in PP , mesenteric lymph nodes , and spleen . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Culture of immature DCs with exogenous Gal 9 markedly increased the surface expression of CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , and HLA DR in a dose dependent manner , although Gal 9 had no or little effect on differentiation of human monocytes into immature DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Upregulation of CD 40 , CD 80 , CD 83 or CD 86 on alveolar macrophages after lung transplantation . ^^^ BACKGROUND : Alveolar macrophages ( AMs ) are known to be poor antigen presenting cells , and lack the accessory molecules such as CD 40 , CD 80 or CD 86 to activate T cells . ^^^ RESULTS : Both CD 86 and CD 80 , as well as CD 83 , a marker of dendritic cells , were enhanced for most AMs during Grade A 2 and A 3 rejection episodes . ^^^ CONCLUSIONS : AM phenotypes showed marked changes during early or late acute rejection episodes , acquiring CD 80 , CD 83 and CD 86 , while CD 40 expression was further enhanced . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression levels of costimulatory molecules ( CD 80 and CD 86 ) , but not of CD 54 and B 7 H1 , were down regulated by EPF . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Increased numbers of B cells expressing the costimulatory molecules CD 80 and CD 86 were also observed in the N . caninum infected mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Human TSLP activates thymic CD11c positive dendritic cells to express high levels of CD 80 and CD 86 . ^^^ This induction depends on peptide major histocompatibility complex class 2 interactions , and the presence of CD 80 and CD 86 , as well as interleukin 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The FcRH4+ memory cells are morphologically distinctive large lymphocytes that express the CD 69 , CD 80 , and CD 86 cell activation markers . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We analyzed 72 formalin fixed and paraffin embedded sequential heart allograft biopsies from 22 patients by quantitative real time reverse transcriptase polymerase chain reaction for the mRNA expression of the costimulatory molecules CD 28 , CD 80 , CD 86 , CD 40 , and CD 154 . mRNA expression levels were correlated to histological grade and time point of rejection . mRNA expression of costimulatory molecules predominantly expressed by antigen presenting cells ( CD 80 , CD 86 , CD 40 ) , correlated with histological grade of cell mediated acute rejection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , our results demonstrate that CD 80 , CD 86 , and PD L 1 molecules are also expressed on T cells that have been activated by simultaneous exposure to anti CD 3 and anti CD 28 mAbs , but PD L 2 and GL 50 molecules were not detectable during the first six days of culture that follow such stimulation . ^^^ Additionally , we provide evidence indicating that the CD 80 , CD 86 , and PD L 1 molecules are functional , since T cells expressing B 7 family molecules are able to stimulate the proliferation of highly purified allogeneic or autologous T cells . ^^^ Anti CD 80 , anti CD 86 , and soluble CD 28 Ig protein could significantly attenuate the proliferation of T cells , whereas anti PD L 1 mAb may lead to the expansion of activated T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The EC 109 DC cells could proliferate slowly in vitro and highly expressed CD 80 , CD 83 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although both CD8alpha+ and CD8alpha subsets increase CD 86 expression , CD 40 was preferentially up regulated on CD8alpha+ DC , and CD 80 was preferentially increased on CD8alpha DC . ^^^ In addition , high levels of CD 80 and CD 86 were found on CD11c ( int ) CD11b+ cells that accumulated in infected organs . ^^^ Rather , infection induced up regulation of CD 80 , CD 86 , and CD 40 on CD11c expressing cells of mesenteric lymph nodes was dependent on TNFR type 1 ( TNFRI ) signaling . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results showed that compared with mature DCs ( mDCs ) generated at high dose of GM CSF plus additional stimulation by lipopolysaccharide , iDCs expressed significantly lower levels of MHC 2 , CD 80 and CD 86 , and their ability to uptake FITC Dextran was stronger but the ability of stimulating proliferation of allogeneic T cells were weaker . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The production of TNF alpha and nitric oxide ( NO ) , the expression of surface molecules CD 80 and CD 86 , and the ability of monocytes to phagocyte bacteria were assessed , in order to evaluate the effect of alginate on cell functionality . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In particular , modulation of the expression of co stimulatory molecules on the targeted APC ; CD 80 , CD 86 , CD 83 and B7RP 1 , play important roles for the effect of the ADP ribosylating CTA 1 based adjuvants for the development of tolerance or active IgA immunity . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results showed that as the disease progresses from before onset to early disease and to late disease , there is a parallel increase in surface markers of B cell activation ( IA / IE , CD 44 , CD 69 , CD 80 and CD 86 ) , in B cell proliferation , and in cell surface and serum IgE . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Treatment of the animals with baicalein , an herbal extract with anti inflammatory and numerous other effects , downregulated the microglia markers CD 68 , CD 80 , and CD 86 , all of which were upregulated in untreated mutant animals . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 are important in the initiation of T cell immunity . ^^^ We have addressed this issue using HLA DR 1 transfectants co expressing CD 80 , CD 86 , or both molecules as stimulators for nave , memory , and activated human CD4+ T cells . ^^^ Both CD 80 and CD 86 efficiently costimulated alloresponses by unseparated peripheral blood CD4+ T cells ; however , CD 86 was substantially inferior in costimulating alloresponses by separated memory T cells , and completely incompetent in costimulating three human T cell clones . ^^^ These results indicate that CD 80 and CD 86 mediate distinct signals in previously activated T cells , and demonstrate that CTLA 4 ligation may dominate the outcome of CD 86 mediated costimulation of activated CD4+ T cells . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Omp 18 induced higher expression of the B 7 ( CD 80 and CD 86 ) costimulatory molecule after 18 hours indicating processing and presentation of the antigen on the surface by bone marrow derived DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
At this time point , the number of myeloid dendritic cells and expression of CD 80 and CD 86 in lungs were decreased compared with three challenges . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The Nef protein of HIV 1 induces loss of cell surface costimulatory molecules CD 80 and CD 86 in APCs . ^^^ We now show that Nef also redistributes the costimulatory molecules CD 80 and CD 86 away from the cell surface in the human monocytic U 937 cell line as well as in mouse macrophages and dendritic cells . ^^^ Furthermore , HIV 1 infected U 937 cells and human blood derived macrophages show a similar loss of cell surface CD 80 and CD 86 . ^^^ Nef colocalizes with MHC class 1 ( MHCI ) , CD 80 , and CD 86 in intracellular compartments , and binds to both mouse and human CD 80 and CD 86 . ^^^ Some Nef mutants defective in MHCI down modulation , including one from a clinical isolate , remain capable of down modulating CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We showed that monocyte derived DC can be productively infected , which results in maturation of the DC judged by the up regulation of CD 80 , CD 83 , CD 86 , and HLA class 2 molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression levels of CD1a , CD 80 , CD 83 , CD 86 and HLA DR as expressed by mean fluorescence intensity ( MFI ) on DC differentiated from immature DC after culture with 1 . 0 microg / ml of SUL 1 for 1 day were enhanced and decreased endocytic activity . ^^^ Cell surface expression of CD 80 , CD 83 and CD 86 on SUL 1 treated DC was inhibited by anti DC SIGN mAb , while anti DC SIGN mAb had no influence on allogeneic T cell proliferation by SUL 1 treated DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Toxoplasma gondii antigen was shown to be taken up by these cells and to increase class 1 and class 2 major histocompatibility complex ( MHC ) , CD 40 , CD 80 and CD 86 surface expression . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OBJECTIVE : This study investigated the effects of lumiliximab on allergen induced immune responses from atopic subjects compared with blocking HLA DR and costimulatory molecules , CD 80 and CD 86 . ^^^ METHODS : Allergen stimulated PBMCs from atopic subjects were pretreated with lumiliximab or antibodies to CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Whereas CTB only delivered the Ag to MZ DC , the ADP ribosyltransferase activity of CT was required for the maturation and migration of DC to the T cell zone , where these cells distinctly up regulated CD 86 , but not CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The aim of the study was to estimate the expression of cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 , CD 152 ) , CD 28 , B7 . 1 ( CD 80 ) , and B7 . 2 ( CD 86 ) molecules on peripheral blood cells in patients with Graves ' disease ( GD ) ( n = 55 , mean age 15 . 5 + / 5 . 1 years ) and nontoxic nodular goiter ( NTNG ) ( n = 55 , mean age 15 . 2 + / 4 . 5 years ) , in comparison with sex and age matched healthy control subjects ( n = 55 , mean age 15 . 2 + / 3 . 9 years ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The main co stimulatory pathway involves the interaction of CD 80 and CD 86 , expressed on the APCs , with their T cell counter receptor , CD 28 and CTLA 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The costimulatory molecules CD 80 , CD 86 and OX40L are up regulated in Aspergillus fumigatus sensitized mice . ^^^ Results showed up regulation of both CD 86 and CD 80 molecules on lung B cells from Af sensitized mice ( 79 % CD86+ and 24 % CD80+ ) and Af / rIL 4 treated mice ( 90 % CD86+ and 24 % CD80+ ) compared to normal controls ( 36 % and 17 % , respectively ) . ^^^ The up regulation of CD 80 , CD 86 and OX40L molecules on lung B cells and macrophages from Af allergen exposed mice suggests a major role for these molecules in the amplification and persistence of immunological and inflammatory responses in ABPA . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Despite the fact that viral infection resulted in phenotypic maturation of moDCs , as shown by the upregulation of cell surface markers and antigen presenting molecules ( MHC 1 and 2 , CD 80 , CD 83 , CD 86 , CD 38 ) , RSV infected moDCs showed a severely impaired capacity to stimulate CD4+ T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Lewis rat hearts were transplanted heterotopically into wild type or IL 12 , CD 80 or CD 86 deficient C57BL / 6 mice . ^^^ Wild type recipients were treated with CD 80 or CD 86 neutralizing Ab with and without daily cyclosporin A ( CsA , 15 mg / kg ) . ^^^ Finally , blockade of CD 86 but not CD 80 rendered xenograft recipients sensitive to daily CsA therapy , leading to indefinite xenograft survival . ^^^ Furthermore , we demonstrate that CD 80 and CD 86 have opposing roles in regulation of xenotransplantation rejection , where CD 80 drives CMR and attenuates AVR while CD 86 drives AVR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These cells were found to express CD 29 , CD 44 , CD 90 , CD 95 , CD 105 , CD 166 , and MHC class , but not CD 14 , CD 34 , CD 40 , CD 45 , CD 80 , CD 86 , CD 117 , CD 152 , or MHC class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
At 2 and 14 d of reperfusion , the following were assessed 1 ) properties of BMMo derived DCs ( i . e . , the amount of generated DCs , differentiation state markers [ CD11c , CD 80 , CD 86 , and Ia ] , and functional state [ MLR and amount of IL 12 produced ] ) , and 2 ) the presence of DCs in the kidney . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , both wild type meningococcal LOS and KDO ( 2 ) lipid A , significantly up regulated CD 80 , CD 83 and CD 86 and released significantly higher amounts of IL 12p70 , IL 6 , IL 10 , TNFalpha , MCP 1 , IP 10 and RANTES . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometry showed polyclonal DC populations after expansion that consisted of CD11c+CD11b+ and CD11c CD11b+ cells that co expressed CD 40 , CD 80 , CD 86 , and MHCII . eDCs were functional in mixed lymphocyte reactions and produced tumor necrosis factor alpha on phytohemagglutinin stimulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC differentiated from monocytes in the presence of apoA 1 showed a decreased expression of surface molecules such as CD1a , CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We examined cell surface expression of five B 7 costimulatory molecules ( B 7 H1 , B 7 DC , B7h , CD 80 and CD 86 ) in human oral squamous cell carcinoma ( SCC ) lines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulation of cells with gliadin , in contrast with other tested food proteins , led to enhanced expression of maturation markers ( CD 80 , CD 83 , CD 86 , and HLA DR molecules ) and increased secretion of chemokines and cytokines ( mainly of IL 6 , IL 8 , IL 10 , TNF alpha , growth related oncogene , MCP 1 , MCP 2 , macrophage derived chemokine , and RANTES ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The immunophenotype of cells altered from CD34+ , CD 38 , CD 40 , CD 80 , CD 86 , MHC 1 , MHC 2 , F4 / 80 to CD 34 , CD38+ , CD40+ , CD80+ , MHC I+ , MHC II+ , F4 / 80 ( low ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , the delivery of sLAg via escheriosomes enhanced the expression of costimulatory signals ( CD 80 and CD 86 ) as determined in peritoneal macrophages obtained from BALB / c mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , DC overexpressing CD40L secreted considerably more IL 12 and expressed higher levels of the co stimulatory molecules , CD 80 , CD 86 and CD 40 , than did DC overexpressing LacZ , 4 1BBL or RANKL . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
GILZ is necessary and sufficient for DEX , IL 10 , and TGFbeta modulation of CD 80 , CD 83 , CD 86 , immunoglobulin like transcript ( ILT ) 3 , and B 7 H1 expression by DCs , and alteration of DC functions . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , for the expression of CD 83 and costimulatory molecules such as CD 40 , CD 80 , and CD 86 , no synergistic effects were observed upon stimulation with Nod plus TLR agonists . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The VIP / PACAP generated DCs are CD11c ( low ) CD45RB ( high ) , do not up regulate CD 80 , CD 86 , and CD 40 following LPS stimulation , and secrete high amounts of IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These CD11c+ DCs did not express CD 123 , CD1a , DC marker ( CMRF 56 ) , CD 40 , CD 80 , or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show that the failure of T lymphocytes to respond to Salmonella infected DC is not simply due to Salmonella induced programmed DC death or interference with up regulation of costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
This loss of APC function correlates with a reduced expression of costimulatory molecules , including programmed cell death ligand ( PD L ) 1 , PD L 2 , CD 40 , CD 80 , CD 86 , and inducible T cell costimulator , and of major histocompatibility complex class 2 on CD11c ( + ) APCs from the airways of ISS treated mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
T reg cell depleted mice had increased numbers of pulmonary myeloid DCs with elevated expression of major histocompatibility complex class 2 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
AS Bcl 2 ODNs induced splenomegaly in association with increased serum IL 12 , expression of CD 80 , CD 83 , CD 86 , and CD 27 , which was attenuated by methylation of the CpG motifs of AS Bcl 2 , suggesting the involvement of immunomodulatory effect of AS Bcl 2 through pDC and B cell although methylated CpG failed to negate the increased antitumor effect of AS Bcl 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Murine bone marrow derived DC efficiently phagocytosed tumor lysate and after the uptake , the phenotype of TP DC was surprisingly comparable to unpulsed DC ( UP DC ) , exhibiting lower levels of CD 80 ( < 51 % ) , CD 86 ( < 43 % ) , and MHC class 2 ( < 59 % ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
At 2 and 14 days of reperfusion , the following properties of PBMo derived DCs were assessed : the amount of generated DCs , surface markers [ CD11c , CD 80 , CD 86 , and MHC 2 ( IA ) ] , and functional status including magnitude of mixed lymphocyte reaction ( MLR ) , production of IL 12 p 70 by DCs , and production of IFN gamma and IL 4 by DC stimulated T cells . ^^^ After 14 days of reperfusion , there was no between group differences in the numbers of DCs derived from PBMo , MLR , expression of CD 80 , CD 86 , and MHC 2 ( IA ) , and production of IL 12 , IFN gamma , and IL 4 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In comparison to DC differentiated with FCS supplementation , DC generated under serum free conditions ( sfDC ) have a more homogeneous phenotype with higher expression of IL 12 and the differentiation and activation markers CD11c , CD 40 , CD 80 , CD 83 , CD 86 , DEC 205 , and MHC class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Studying the status of accessory molecules implicated in T cell stimulation by M , it could be shown that B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) remained unchanged , whereas CD 40 was down regulated and CD 54 ( =ICAM 1 ) slightly up regulated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : After six days of culture , the monocytes developed significant dendritic morphology and a portion of cells expressed CD1a , CD 80 and CD 86 , features of DCs . ^^^ TNFalpha treatment induced DCs maturation and up regulation of CD 80 , CD 86 and CD 83 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Direct examination of the serosal disposed layers showed a significant fraction of the DCs to express DEC 205 / CD205 , CD11c , Langerin / CD207 , Fcgamma receptor / CD16 / 32 , CD 14 , and low levels of activation markers , CD 25 , CD 80 , CD 86 , and CD 95 . ^^^ In vivo , these DCs responded to two microbial stimuli , systemic LPS and oral live bacteria , by up regulating CD 80 , CD 86 , DEC 205 , and Langerin within 12 h . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Splenic DC strongly up regulated CD 86 and CD 40 but not CD 80 as soon as 8 h after CLP . ^^^ In contrast , lymph node DC equally increased the expression of CD 86 , CD 40 , and CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , heptanol significantly inhibited the LPS plus IFN gamma induced up regulation of the other costimulatory ( i . e . , CD 80 and CD 86 ) and MHC class 2 molecules expressed by BMDCs , and it significantly reduced their allostimulatory capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , NYT dose dependently increased the surface expression of major histocompatibility complex ( MHC ) class 2 ( MHC 2 ) , CD 40 , CD 54 , CD 80 , and CD 86 by murine bone marrow derived DCs and triggered their robust production of IL 1beta , IL 6 , IL 12 , tumor necrosis factor alpha , and macrophage inflammatory protein 1alpha . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Herein , we provide evidence to support the hypothesis that dendritic cells derived from NOD mice and engineered ex vivo to exhibit suppressed expression of the CD 40 , CD 80 , and CD 86 costimulatory molecules motivate an increase in the prevalence of regulatory CD 4 ( + ) CD 25 ( + ) T cells via interleukin ( IL ) 7 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It was possible to generate mature DCs from monocytes with the aid of CD40L in both cases , but decreased expression of HLA DR , CD 80 , and CD 86 markers was observed upon posttransplantation analyses in the patient with recurrent HCV . ^^^ CONCLUSION : Loss of the proliferative response as well as INF gamma and TNF alpha production , together with a decreased HLA DR , CD 80 , and CD 86 ( markers of mature DCs ) , indicated an inadequate immune response to viral progression in the liver transplant recipient with relapsing HCV infection . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of the co stimulatory molecule CD 80 but not CD 86 increased on both DC subsets when stimulated with cyclosporine . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results showed that , on B cells , GroES and GroEL stimulated the expression of CD 86 but did not induce the increase of the CD 80 expression . ^^^ GroES and GroEL also stimulated the expression of CD 80 and CD 86 on macrophages . ^^^ The same HSPs did not modify the expression of CD 80 and CD 86 on cells having characteristics of activated macrophages , the A THP 1 cell line . ^^^ DnaK did not induce any increase in the expression of CD 80 and CD 86 on lymphocytes or macrophages . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Culture of immature DCs with exogenous Gal 9 markedly increased the surface expression of CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , and HLA DR in a concentration dependent manner , although Gal 9 had no effect on differentiation of human monocytes into immature DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Different from GL S , GL M enhanced maturation of DCs in terms of upregulation of CD 40 , CD 80 , and CD 86 , and also reduced fluorescein isothiocyanate dextran endocytosis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , significant amounts of costimulatory molecules CD 40 , CD 80 and CD 86 could be detected on nmDC that expressed more CD 80 compared with omDC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Interestingly , in contrast to mature DCs derived from TNF alpha induced immature DC cultures without PsL EGFmAb , immature DCs treated with PsL EGFmAb for 7 days were completely blocked their maturation , as evidenced by decreased expression of costimulatory molecules CD 80 , CD 86 , and CD 83 , inhibited production of IL 12 , and inability to activate nave T cells in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Type 1 myeloid ( CD11c ( + ) , CD11b ( + ) , B 220 ( ) , CD8alpha ( ) ) DC made up the largest ( 40 45 % ) population and all DC expressed low levels of CD 80 , CD 86 , and CD 40 , and had high endocytic activity consistent with an immature phenotype . ^^^ The majority ( > 85 % ) of DC in the colon and MLN of IL 2 ( / ) mice were type 1 myeloid , and expressed high levels of MHC class 2 , CD 80 , CD 86 , CD 40 , DEC 205 , and CCR 5 molecules and were of low endocytic activity consistent with mature DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The upregulation by FSL 1 of the expression of CD 80 , CD 86 and the MHC class 2 molecule IA ( b ) was dose and time dependent on the surfaces of C57BL / 6 derived bone marrow derived dendritic cells but not on the surface of TLR 2 ( / ) derived bone marrow derived dendritic cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both DEN 2 and LAV 2 increased the expression of the phenotypic markers CD 80 , CD 86 , CD 40 , CD1a , HLA ABC and CD 83 , demonstrating cellular activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
OBJECTIVES : To compare the expression of CD 40 , CD 80 , CD 86 , MHC class 2 and intercellular adhesion molecule 1 ( ICAM 1 ) , and the ability to induce allogeneic T lymphocyte proliferation in vitro , of a CD 40 OSCC cell line , its CD40+ transfected derivative and null transfectants . ^^^ However , the CD40+ transfectants were the most immunologically potent and were the only cells to show increased expression of CD 86 ( as well as further upregulation of CD 40 and a statistically insignificant rise in CD 80 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Increased expression of CD 40 , CD 80 , and CD 86 by B cells was apparent within 24 h of exposure to C 274 and persisted for up to 1 week . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Levels of the soluble forms of CD 80 , CD 86 , and CD 83 are elevated in the synovial fluid of rheumatoid arthritis patients . ^^^ The release of soluble forms of CD 80 ( sCD 80 ) , CD 86 ( sCD 86 ) , and CD 83 ( sCD 83 ) provide a potentially powerful immunoregulatory mechanism . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
KM+ induced a smaller inflammatory reaction in the air pouch model and was able to inhibit differentiation of dendritic cells ( BMDC ) , but to induce maturation by enhancing the expression of MHC 2 , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometry revealed CD11b ( + ) / Gr 1 ( + ) as a heterogeneous myeloid suppressor cell also expressing low levels of MHC class 1 and 2 , CD 80 , CD 86 , CD 31 , and others . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In this study , we show that BLS stimulates bone marrow dendritic cells from mice in vitro to up regulate the levels of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) and major histocompatibility class 2 Ag . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Under explant culture system , SHPS 1 Fc treatment suppressed the expression of CD 80 and CD 86 of LCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here , we show that dendritic cells ( DC ) derived from CD patients homozygous for this mutation respond normally to purified Toll like receptor ( TLR ) ligands but fail to up regulate the costimulatory molecules CD 80 and CD 86 in response to the NOD 2 ligand muramyl dipeptide ( MDP ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It profoundly increased CD 40 , CD 54 , CD 80 , CD 86 , and MHC class 2 expression in murine bone marrow ( BM ) derived myeloid DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results demonstrate that , in contrast to the rwt virus , rM51R M virus induced the maturation of myeloid DC ( mDC ) populations , as indicated by an increase in the surface expression of CD 40 , CD 80 , and CD 86 as well as the secretion of interleukin 12 ( IL 12 ) , IL 6 , and type 1 IFN . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
When activated via TLRs , antigen presenting cells ( APCs ) release elevated levels of pro inflammatory cytokines ( TNF alpha , IL 1 , IL 6 , IL 8 , and IL 12 ) , chemokines , and nitric oxide ( NO ) and show increased expression of co stimulatory molecules ( CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cytotoxic T lymphocyte antigen 4 ( CTLA 4 ; CD 152 ) is a member of the immunoglobulin gene superfamily with strong homology to the receptor CD 28 with which it shares the ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
PR 3 treated DCs express high levels of CD 83 , a DC restricted marker of maturation , CD 80 and CD 86 , and HLA DR . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
EtOH reduced surface expression of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) but not that of coinhibitory CD 274 ( B 7 H1 ) on resting or CpG stimulated DC subsets . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Artificially enforced expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on tumor cells renders them more immunogenic by triggering the CD 28 receptor on T cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Further culture of myeloid cells in serum free medium with GM CSF and IL 4 generated cells that had typical dendritic morphology ; expressed high levels of MHC class 1 and 2 molecules , CD1a , CD11c , CD 80 , CD 86 , DC SIGN , and CD 40 ; and were capable of Ag processing , triggering naive T cells in MLR , and presenting Ags to specific T cell clones through the MHC class 1 pathway . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Gastric epithelial cells ( GEC ) play an important role during the host response , and their expression of class 2 MHC and costimulatory molecules such as CD 80 and CD 86 suggests their role in local Ag presentation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Perfusate MDC expressed less DC LAMP ( P = 0 . 000 ) , CD 80 ( P = 0 . 000 ) , CD 86 ( P = 0 . 003 ) , and CCR 7 ( P = 0 . 014 ) than mature hepatic lymph node ( LN ) MDC , and similar CD 86 ( P = 0 . 140 ) and CCR 7 ( P = 0 . 262 ) as and more DC LAMP ( P = 0 . 007 ) and CD 80 ( P = 0 . 002 ) than immature blood MDC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Stimulation with H . pylori induced maturation of DC , i . e . up regulation of the chemokine receptors CCR 7 and CXCR 4 and the maturation markers HLA DR , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The early activation marker CD 69 was induced at 4 h in NK cells > B cells > T cells > monocytes whereas at 24 h CD 80 and CD 86 levels were augmented on monocytes and IL 12 was induced ; HLA DR levels increased on both B cells and monocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Dendritic cells generated from monocytes from EBC patients showed a significantly lower expression of the phenotype associated antigens CD1a , CD 83 , CD 80 , CD 86 and CD 54 than the dendritic cells from healthy controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Lupus DCs showed an altered ex vivo costimulatory profile , with a significant increase in the expression of CD 40 , decreased expression of CD 80 and CD 54 , and normal expression of CD 86 . ^^^ DCs from young lupus prone NZM 2410 mice , before the development of the disease , expressed normal levels of CD 80 and CD 86 but already overexpressed CD 40 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the present study , MUTZ 3 derived DC ( MuDC ) showed high level expression of HLA class 2 molecules , CD 80 and CD 86 , and were able to function as potent antigen presenting cells as previously reported . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , impaired expression of co stimulatory molecules including CD 80 , CD 86 , and CD40L may contribute to low polyfunctional CD4+ T cell as well CD8+ T cell activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Co culture resulted in DC with reduced expression of MHC class 2 , CD 86 , and CD 80 , and poor T cell stimulatory capacity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The surface molecules CD 25 , CD 40 , CD 80 and CD 86 were up regulated upon incubation of the B CLL cells with IMT 504 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expressions of surface marker CD 40 , CD 80 , and CD 86 in the GM CSF treatment group were also higher than those of the control group . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
HSP70 ) induced maturation of bone marrow derived dendritic cells ( DCs ) of wild type ( WT ) C57BL / 6 mice as evidenced by an increase in surface expression of MHC class 1 and 2 molecules and costimulatory molecules such as CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immunostimulation was evaluated in vitro by flow cytometric measurements of the CD 80 and CD 86 activation markers found on CD19+ murine splenocytes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We show in this report that JEV infection of mouse brain astrocytes results in induction of the nonclassical MHC Class Ib genes , H 2T23 , H 2Q4 and H 2T10 in addition to MHC 1 , Type 1 ( alpha / beta ) IFNs , TAP 1 , TAP 2 , Tapasin , LMP 2 , LMP 7 and LMP 10 but not IFNgamma , CD 80 , CD 86 and MHC 2 genes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The VLP induced an increase in expression of CD 40 , CD 80 and CD 86 but required an adjuvant , CpG DNA oligo deoxy nucleotides ( ODN ) motifs , to enhance these responses . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
C . jejuni induced the maturation of dendritic cells over 24 h , as indicated by up regulation of cell surface marker proteins CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
F . tularensis LVS also induced in a dose dependent manner an up regulation in the expression of the costimulatory molecules CD 80 and CD 86 and of CD 40 and the major histocompatibility complex class 2 molecules on dendritic cells from wt and TLR 4 / but not TLR 2 / mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Cells matured normally ; up regulation of CD 40 , CD 80 , CD 83 , CD 86 and HLA DR was not affected by adenoviral transduction . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The ability of eosinophils to enhance cytokine generation was independent of their source ( blood vs airway ) , activation by GM CSF , or detectable expression of human leukocyte antigen ( HLA ) DR , CD 80 , or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression levels of CD1a , CD 80 , CD 83 , CD 86 and HLA DR on T cadinol primed dendritic cells or calamenene primed dendritic cells were enhanced with a concomitant decrease in endocytic activity . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Although expression levels of DC associated surface antigens , including CD 80 , CD 83 , and CD 86 , were significantly higher on HHV 6 infected monocyte derived DCs than on DCs derived from mock infected monocytes , antigen presenting capacity was significantly lower in the former group . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
These results demonstrate that genes associated with phagocytosis ( CD 36 , CD 206 , and CD 209 ) are decreased and genes associated with proinflammatory chemokines ( CCL 20 , CCL 5 , and CCL 19 ) , cytokines [ interleukin ( IL ) 27 , IL 23A , IL 12A , and IL 12B ] , and costimulatory molecules ( CD 40 , CD 54 , CD 80 , and CD 86 ) are increased . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We showed that both gangliosides impaired spontaneous LC maturation induced by a short in vitro culture , as assessed by significant down regulation of co stimulation ( CD 40 , CD 54 , CD 80 , CD 86 ) and maturation markers ( CD 83 , CCR 7 ) , which correlated to an impaired ability of the cells to mount allogeneic T cell proliferation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Using flow cytometry , the cells were analyzed for the expression of costimulatory molecules , such as CD 80 , CD 86 , and CD 152 ( CTLA 4 ) . ^^^ CONCLUSION : These results suggest that LIMC over expressing costimulatory molecules such as CD 80 and CD 86 appears to play a role in the pathogenesis of AIH . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
While purified CD123+ plasmacytoid dendritic cells from adults up regulated co stimulatory molecules CD 80 and CD 86 with IL 3 alone those from neonates required the addition of CpG DNA to reach adult levels . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The frequency of circulating MDC and PDC , and the expression of CD 83 , CD 123 , CD 80 , CD 86 , and CD 40 were analyzed by flow cytometry . ^^^ The expression of CD 83 , CD 80 , and CD 86 was lower in PPMS patients . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : Immature in vitro derived DC exhibited robust phagocytic activity , and mature DC demonstrated high expression of CD 80 , CD 83 , CD 86 and the chemokine receptor CCR 7 , important for DC migration to local lymph nodes . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Integration of CD 28 and CTLA 4 function results in differential responses of T cells to CD 80 and CD 86 . ^^^ CD 80 and CD 86 have the capacity to either stimulate or inhibit T cell responses through their receptors CD 28 and cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) . ^^^ Blockade of CD 80 and CD 86 in autoimmune disease settings has revealed distinct outcomes , yet the differential functions of CD 80 and CD 86 are still unclear . ^^^ Both CD 80 and CD 86 demonstrated the capacity to costimulate T cell proliferation . ^^^ Once cell division had been initiated , all T cells undergoing cell division expressed CTLA 4 , irrespective of whether CD 80 or CD 86 costimulation was used . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Flow cytometric analysis of draining lymph node DC costimulatory molecules and activation markers from infected mice indicated a lack of upregulation of major histocompatibility complex class 2 , CD 80 , CD 86 , and CD 40 during infection , consistent with a tolerizing condition . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 , CD 83 , CD 86 and HLA DR expression on naive and stimulated myeloid dendritic cells from peripheral blood were analyzed . ^^^ No differences in CD 80 , CD 83 , CD 86 and HLA DR expression were found on naive and stimulated myeloid dendritic cells from HCC patients and healthy controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The co stimulatory molecules MHC 2 , CD 40 , CD 80 , and CD 86 were expressed in DC aggregates . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : The DC from CHB patients had a lower expression of costimulatory molecules CD 80 , CD 86 and impaired allogeneic mixed lymphocyte reaction capacity compared to those from normal controls . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Of note , we found that CD 83 , CD 80 , CD 86 , CD 40 , and interleukin 12 upregulation were significantly impaired on K ( 5 ) 1 . 3 and K ( 5 ) 1 . 5 blockade . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The immunostaining revealed positivity for CD 45 , CD 20 , CD 22 , CD79a , IgA 2 , CD 80 , CD 86 , CD 74 , and BCL 6 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , this increased uptake did not result in an increase in expression of CD 80 or CD 86 on the surface of the MoDCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Hyperthermia increases the expression of co stimulatory molecules such as CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Immature monocyte derived DC ( iMDDC ) failed to undergo phenotypic ( CD 80 , CD 83 , CD 86 ) or functional ( T cell activation ) maturation in response to exposure to DEP ( 0 . 001 100 mug / ml ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
A fourth cell line , unable to form tube like structures in Matrigel , produced large granulocytic cells expressing CD11b and CD 86 but not CD11c and CD 80 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The expression of interferon gamma ( IFN gamma ) , Interleukin 12p40 ( IL 12p40 ) , costimulatory molecules CD 40 , CD 80 and CD 86 and MHC class 2 were up regulated in DLN of CIA induced C57BL / 6 mice . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We have previously demonstrated that CD4+ CD25+ natural regulatory T cells ( Treg cells ) induce down modulation of CD 80 and CD 86 ( B 7 ) molecules on dendritic cells ( DCs ) in vitro . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In the presence of apigenin , CD 80 , CD 86 , and major histocompatibility complex class 1 and 2 molecules , expressions on DC were significantly suppressed , and lipopolysaccharide ( LPS ) induced interleukin ( IL ) 12 expression was impaired . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Additionally , lung DC from mice infected for 7 days showed increased expression of the maturation markers CD 80 , CD 86 , and major histocompatibility complex class 2 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 5 ( ) CD 4 ( ) CD 86 ( ) CD 80 ( ) CD134L ( + ) VDCs from infected , but not noninfected , rats spontaneously released large amounts of interleukin 12 ( IL 12 ) and tumor necrosis factor alpha , whereas all VDC subsets released comparable levels of IL 10 and IL 2 cytokines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The results showed that MPA not only inhibited the expression of major histocompatibility complex ( MHC ) class 2 and costimulatory molecules CD 80 and CD 86 but upregulated B 7 DC expression on mature DC induced by LPS . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The rAAV altered DC displayed higher levels of CD 80 , CD 83 , CD 86 , and CD 1a than control DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In addition , the costimulatory molecules CD 80 and CD 86 were up regulated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
We observed high levels of CD 80 expression by Gr 1+CD11b+ cells from spleen , ascites , and tumor tissue of mice bearing 1D8 ovarian carcinoma , whereas CD 40 and CD 86 were absent . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Both LPS and CT stimulated increases in CD 80 and CD 86 expression on OVA+CD8alpha ( low ) DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Kinetic studies showed a subset and tissue specific expansion of cDC and upregulation of CD 80 and CD 86 on splenic and mesenteric lymph node ( MLN ) cDC after intragastric infection . ^^^ Expansion of pDC was more prolonged than cDC , and pDC upregulated CD 86 and MHC 2 , but not CD 80 , in both the spleen and MLN . cDC were an important source of IL 12 but not TNF alpha during infection , while pDC made neither of these cytokines . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
DC were incubated with PL resulting in up regulation of MHC 2 , CD 40 , CD 80 , and CD 86 expression and production of TNFalpha and IL 12 ( p 70 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Mature PDC expressed significantly higher levels of inducible costimulator ligand ( ICOS L ) , but similar levels of CD 80 and CD 86 , when compared to mature monocyte derived DC ( moDC ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 4 ( + ) PHA blasts express MHC class 1 and 2 , and also CD 80 and CD 86 and are thus expected to serve as potent antigen presenting cells . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of costimulatory / adhesion molecules ( CD1a , CD11c , CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , CD 123 , HLA class 1 and 2 ) was assessed by flow cytometry and mRNA for cytokines ( IFN gamma , IL 10 , IL 4 , TGF beta ) with real time PCR . ^^^ Results : 1 ) high expression was observed for HLA 1 and 2 class , moderate for CD 40 , CD 83 , CD 86 and low or no expression for CD 80 , CD 54 , CD1a , CD11c and CD 123 ; 2 ) we found expression of mRNA for IFN gamma , IL 10 , IL 4 and TGF beta in blasts cells ( but not in all specimens ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
When monocyte derived DCs were obtained from the patient after having recovered from the Mycobacterium avium complex infection , they expressed normal levels of CD1a and CD 80 and were negative both for CD 83 and for CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC SIGN , and that this increased after stimulation in vitro with interleukin 4 and CD 40 ligand , with enhanced expression of activation and co stimulatory molecules CD 23 , CD 58 , CD 80 , and CD 86 , and CD 22 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Our results showed that LMP 2 gene transfer did not alter the typical morphology of mature DC , and the representative phenotypes of mature DC ( CD 80 , CD 83 , and CD 86 ) were highly expressed in rAd LMP 2 DCs . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Furthermore , depending on the mouse strain used , increased expression of MHC class 2 and costimulatory molecules such as CD 40 , CD 80 , and CD 86 was associated with exposure to SFA 8 , but not Ber e 1 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
More importantly , phagocytosis of the targeting microparticles neither altered the functionality of immature DCs nor the phenotypic expression of DC specific cell surface molecules , CD 80 , CD 86 , CD 40 and CD 54 ( ICAM 1 ) , suggesting that uptake of the targeting microparticles by themselves did not induce DC maturation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In contrast , the serum levels of tumor necrosis factor alpha and interleukin 6 and spinal interleukin 6 levels were elevated , and the expression of major histocompatibility complex class 2 molecules , CD 80 , and CD 86 on splenic dendritic cells were enhanced early after infection . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Hybrid cell lines generated from three marrow derived tumour cells consistently expressed both MHC class 1 and class 2 molecules , a range of accessory and T cell co stimulatory ligand molecules , including CD 80 and CD 86 , and directly stimulated markedly enhanced T cell proliferative responses in vitro , as compared with the parent tumour cell lines . ^^^ By contrast , hybrid cells derived from three solid tumours only expressed MHC class 2 when the parent tumour cell line expressed MHC class 2 and consistently failed to express CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Signal two or co stimulation is mainly provided by the triggering of CD 28 on the T cell by CD 80 and CD 86 molecules on the DC . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD4+ T cell populations in the peripheral blood were analysed for the expression of co stimulatory markers CD45RO , CD 70 , CD 80 , CD 86 , CD 137 , CD137L , CD 134 , CD 152 , CD 154 and ICOS . ^^^ SLE patients showed an increased frequency of peripheral CD4+ T cells expressing high levels of CD 80 , CD 86 and CD 134 compared to healthy controls ( 7 . 1 + / 1 . 5 % versus 1 . 7 + / 0 . 9 % ; P < 0 . 005 ; 2 . 3 + / 0 . 4 % versus 1 . 0 + / 0 . 2 % ; P = 0 . 008 , 20 . 2 + / 2 . 0 % versus 10 . 6 + / 1 . 9 % ; P < 0 . 005 , respectively ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
It was found that B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression remained unchanged , whereas CD 40 was down regulated and CD 54 ( = ICAM 1 ) was slightly up regulated . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
After lipopolysaccharide ( LPS ) stimulation , F4 / 80+ SPMs expressed significantly higher levels of CD 86 , CD 40 , or MHC 2 than F4 / 80+ PEMs , but not CD11c , CD 80 , CD 54 , or CD 23 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The VIP / PACAP generated DCs are CD11clowCD45RBhigh , do not upregulate CD 80 , CD 86 , and CD 40 following lipopolysaccharide ( LPS ) stimulation , and secrete high amounts of IL 10 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
When triggered by specific antigen , these Tregs act back on immature DCs to block the upregulation of CD 80 and CD 86 costimulatory molecules . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
RESULTS : All the TLR agonists induced maturation of DC as evidenced by increased expression of CD 83 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
There was an upregulation of the costimulatory molecules major histocompatibility complex 2 , CD 40 , CD 80 , CD 86 , and CD 205 in the DC after intestinal manipulation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
In vitro , NIK increases dendritic cell antigen presentation in allogeneic and antigen specific T cell proliferation assays by potently activating NF kappaB and consequently up regulating the expression of cytokines ( TNF alpha , IL 6 , IL 12 , IL 15 , and IL 18 ) , chemokines [ IL 8 , RANTES ( regulated on activation , normal T cell expressed and secreted ) , macrophage inflammatory protein 1alpha , monocyte chemoattractant protein 1 , and monocyte chemoattractant protein 3 ] , MHC antigen presenting molecules ( class 1 and 2 ) , and costimulatory molecules ( CD 80 and CD 86 ) . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
The generation of this population was paralleled by higher expression of the costimulatory molecules CD 80 , CD 86 , and B 7 DC , but not B 7 RP , PD 1 , and B 7 H1 on CD11c ( + ) CD 4 ( + ) dendritic cells whereas CD11c ( + ) CD8alpha ( + ) dendritic cells were not altered . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
CD 80 and CD 86 have been shown to play a critical role in the optimal activation of T cells . ^^^ Although these two molecules bind the same ligand , CD 28 , the question of whether CD 80 and CD 86 provide unique signals or serve redundant roles remains controversial . ^^^ Previous studies have suggested that CD 80 binding to CD 28 may be superior to CD 86 for the activation of naive CD8+ T cells . ^^^ Our study demonstrates a previously unappreciated role for CD 80 , its superiority over CD 86 in promoting CD 25 expression , increasing both the number of cells that express CD 25 and the level expressed on a per cell basis . ^^^ These findings provide new insights into the role of CD 80 vs CD 86 and have important implications for the design of vaccines and immunotherapeutics aimed at the generation of a robust CD8+ T cell response in vivo . . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Expression of major histocompatibility complex ( MHC ) class 1 molecules , MHC class 2 , CD 80 , CD 86 , CD 40 , CD 70 , B7H1 , B7DC , B7H2 , OX40L , and 4 1BBL on Y 79 cells was measured by reverse transcription polymerase chain reaction ( RT PCR ) and flow cytometric analysis . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
Moreover , ORFV IL 10 inhibited the upregulation of DC cell surface activation and maturation markers MHC 2 , CD 80 , CD 83 and CD 86 and inhibited the capacity of MoDCs to activate CD 4 ( + ) T cells in an oxidative mitogenesis assay . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
However , in CD 100 / mice , dendritic cell CD 86 ( but not CD 80 ) up regulation was significantly attenuated . ^^^ Following i . p . immunization , CD 86 , but not CD 80 , promotes early Ag specific TCR transgenic DO11 . 10 CD4+ cell proliferation and IFN gamma production , suggesting that CD 100 expression enables full expression of CD 86 and consequent CD4+ cell activation . ^^^
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P42081	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.71112434
The interactions between CD 80 and CD 86 on antigen presenting cells and CD 28 on T cells serve as an important costimulatory signal in the activation of T cells . 0.71112434^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recently , diversities between CD 80 and CD 86 in expression , regulation , and function have been reported in certain cell populations and murine experimental disease models . ^^^ To investigate the possible differential role of CD 80 and CD 86 in the development of lupus , we treated lupus prone NZB / W F 1 mice with specific monoclonal antibodies ( mAb ) against CD 80 , CD 86 , or both . ^^^ The treatment with a combination of anti CD 80 and CD 86 mAb before the onset of lupus completely prevented autoantibody production and nephritis , and prolonged survival . ^^^ Interestingly , we found that anti CD 86 mAb alone , but not anti CD 80 mAb , efficiently inhibited autoantibody production . ^^^ The incomplete reduction of IgG2a anti dsDNA antibody by anti CD 86 mAb was compensated by the addition of anti CD 80 mAb . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis of the cells during a 2 week culture revealed a loss of CD 14 and CD 34 expression , a concomittent increase of CD1a , CD11a , b and c , CD 44 , CD 45 , CD 54 , HLA class 1 and 2 , and intermediate levels of CD 26 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Finally , CD40L CD 40 interactions do not induce CD 80 , CD 86 , or major histocompatibility complex class 2 expression on HUVEC in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory molecules B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and interleukin 12 cytokine in multiple sclerosis lesions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In particular , CD 40 triggering induced maintenance of high levels of major histocompatibility complex class 2 antigens and upregulation of accessory molecules such as CD 58 , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It is interesting to note that mAbs against CD 86 completely prevented the binding of CTLA 4 Ig in the presence of mAbs against CD 80 and bound to a B70 / B7 2 transfected fibroblast cell line , demonstrating that the B70 / B7 2 antigen is identical to CD 86 . ^^^ This inhibitory effect was enhanced to > or = 90 % when a combination of anti CD 80 and anti CD 86 mAbs was used . ^^^ The present results demonstrate that D Lc express , in addition to CD 80 , the other ligand for CTLA 4 , CD 86 ( B70 / B7 2 ) , which plays a primordial role during D Lc induced alloreaction . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 ) and CD 86 ( B 70 ) provide similar costimulatory signals for T cell proliferation , cytokine production , and generation of CTL . ^^^ Recently , it has been demonstrated that CD 28 interacts with two different ligands , designated CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) . ^^^ We have produced stable transfectants that express CD 80 , CD 86 , or both ligands and have examined their ability to costimulate T cell proliferation , cytokine production , and the generation of CTL . ^^^ When we used small , resting human peripheral blood T cells as responders , both CD 80 and CD 86 transfectants efficiently costimulated anti CD 3 mAb induced proliferation and the secretion of IL 2 and IFN gamma . ^^^ Additionally , both CD 80 and CD 86 transfectants were able to generate functional CTL . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we demonstrate that B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are also expressed on murine T cells in the absence of major histocompatibility complex class 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential modulation of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression . ^^^ Such cellular interactions are mediated by surface molecules including MHC class 2 Ags ( DR ) and CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^ Normal and psoriatic skin derived DDCs express high levels of CD 86 over CD 80 , and the overall hierarchy is DR > CD 86 > CD 80 , whereas cultured monocytes express low and equivalent levels of CD 80 and CD 86 . ^^^ If Ab is added to GM CSF at the initial period of cultivation , DDCs that emigrate have lower levels of CD 86 without any detectable effect on CD 80 or DR expression and display a reduced capacity to stimulate either superantigen driven or alloantigen responsive T cells . ^^^ When IL 10 was added at the beginning of culture , DDCs had significantly lower levels of CD 86 , without any effect on CD 80 or DR expression , and like anti GM CSF treated cells , these DDCs had approximately a 50 % reduction in their T cell stimulating capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Human B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) bind with similar avidities but distinct kinetics to CD 28 and CTLA 4 receptors . ^^^ B 7 0 or B 7 2 ( CD 86 ) is a T cell costimulatory molecule that binds the same receptors ( CD 28 and CTLA 4 ) as B 7 1 ( CD 80 ) , but shares with it only approximately 25 % sequence identity and is expressed earlier during an immune response . ^^^ Here we show that human CD 86 maintains similar ( within approximately 2 to 3 fold ) overall receptor binding and T cell costimulatory properties as CD 80 . ^^^ However , CD 80 and CD 86 did not bind equivalently to CTLA 4 : CD 80 bound Y100A , a form of CTLA4lg with a mutation in the CDR 3 like region , > 200 fold better than did CD 86 ; inhibition of CD 80 mediated cellular responses required approximately 100 fold lower CTLA4lg concentrations ; and CD 80 CTLA4lg complexes dissociated 5 to 8 fold more slowly , Thus , CD 80 and CD 86 utilize different binding determinants and have different kinetics of binding to CD 28 and CTLA 4 . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immunoglobulin fold characteristics of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD28 / CTLA 4 ligands : the gene encoding CD 86 ( B70 / B7 . 2 ) maps to the same region as CD 80 ( B7 / B7 . 1 ) gene in human chromosome 3q13 q 23 . ^^^ The CD 86 gene is , therefore , located in the proximity of the CD 80 ( B7 / B7 . 1 ) gene , the first identified ligand for CD 28 and CTLA 4 , previously mapped to chromosome 3q13 . 3 q 21 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 are homologous T cell receptors of the immunoglobulin ( Ig ) superfamily , which bind B 7 molecules ( CD 80 and CD 86 ) on antigen presenting cells and transmit important costimulatory signals during T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Engagement of the TCR / CD3 complex together with ligation of CD 28 by its counterstructures B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on APC are required for mitogenic T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
One of these residues , Y 87 , is conserved in all CD 80 and CD 86 cloned from various species . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The B 7 related molecules CD 80 and CD 86 are expressed on antigen presenting cells , bind the homologous T cell receptors CD 28 and CTLA 4 , and trigger costimulatory signals important for optimal T cell activation . ^^^ All four molecules are immunoglobulin superfamily members , each comprising an extracellular Ig variable like ( IgV ) domain , with CD 80 and CD 86 containing an additional Ig constant like ( IgC ) domain . ^^^ Despite limited sequence identity , CD 80 and CD 86 share similar overall receptor binding properties and effector functions . ^^^ We have identified , by site directed mutagenesis of soluble forms of CD 80 and CD 86 , residues in both the IgV and IgC domains that are important for CTLA4Ig and CD28Ig binding . ^^^ In general , mutations had a similar effect on CTLA4Ig and CD28Ig binding , thereby indicating that both receptors bind to overlapping sites on CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interactions between CD28 / CTLA 4 on T cells and CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) counter receptors provide crucial costimulatory signals for TCR alpha beta+ lymphocytes . ^^^ Alloreactivity of CD 28 / and CD28+ / Tg gamma delta+ thymocytes could not be blocked with mAbs against CD 80 and CD 86 ligands . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Precursor derived DC express high levels of CD 86 ( B 7 2 ) and HLA DR but no CD 80 ( B 7 1 ) , whereas mature DC have high levels of expression of all three markers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The second , or costimulatory signal , can be provided by ligation of the B lymphocyte activation antigens B 7 1 ( CD 80 ) and B7 . 2 ( CD 86 ) to TCR antigen CD 28 . ^^^ The cDNAs for both CD 80 and CD 86 have been isolated and are predicted to encode type 1 membrane proteins of the immunoglobulin ( Ig ) superfamily . ^^^ Here we report that the genomic organization of CD 86 reflects its functional structure , and is similar to that found for CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In these experiments , the expression and function of the CD28 / CTLA 4 ligands B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) were examined on human blood DC . ^^^ Resting DC populations directly isolated by immunodepletion of lineage marker positive cells lacked cell membrane expression of CD 80 and expressed little or no CD 86 , although CD 86 , but not CD 80 mRNA was detected by reverse transcription polymerase chain reaction analysis . ^^^ In contrast , low density DC isolated after culture in vitro strongly expressed CD 86 surface protein , but expressed limited or no CD 80 , although mRNA for both molecules were detected . ^^^ Short term culture of directly isolated DC up regulated both CD 80 and CD 86 expression . ^^^ Analysis of the kinetics of CD28 / CTLA 4 ligand induction showed that surface CD 86 was present within 8 h , whereas CD 80 antigen was first detected after 24 h of culture . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Production of the various cytokine mRNAs following alloantigen stimulation was inhibited by CTLA4Ig and thus remained under the regulation of CD 80 and CD 86 expressed on the EBV LCL . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
All three clone types were able to activate B cells as shown by up regulation of CD 25 , CD 80 and CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interleukin 10 differentially regulates B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression on human peripheral blood dendritic cells . ^^^ Flow cytometric analysis indicated that rIL 10 did not modify the expression of ICAM 1 ( CD 54 ) and B 7 1 ( CD 80 ) , but decreased HLA DR and B 7 2 ( CD 86 ) expression at the DC surface . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Approximately 2 to 8 million cells with the characteristics of dendritic cells could be obtained : phase contrast microscopy revealed the typical cytoplasmic processes or veils ; phenotypic analysis confirmed expression of dendritic cell associated molecules , including MHC class 2 , CD1a , CD 4 , ICAM 1 ( CD 54 ) , LFA 3 ( CD 58 ) , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , and absence of T cell , B cell and monocyte markers ; in addition , these cells rapidly attached to and migrated on collagen type 1 coated surfaces . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Distinct roles for B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in the initiation of experimental allergic encephalomyelitis . ^^^ The present studies examined the role of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulation in the prototypic autoimmune disorder , experimental allergic encephalomyelitis ( EAE ) . ^^^ In adoptively transferred EAE , in vitro activation of myelin basic protein ( MBP ) specific lymph node cells was inhibited by the combination of anti CD 80 plus anti CD 86 , but not individually . ^^^ However , in actively induced disease , one injection of anti CD 80 significantly reduced disease , while anti CD 86 exacerbated disease . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Unexpectedly , tumor cells of 5 cases expressed at least one member of the B 7 family ( CD 80 , CD 86 , and B 7 3 ) and many lymphoid cells expressing the corresponding counter receptor , CD 28 , were detected in all cases . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) induce long term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro . ^^^ In this report , the co stimulatory signals provided by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) were compared to CD 28 ligation by mAb . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both CD 28 ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) activate phosphatidylinositol 3 kinase , and wortmannin reveals heterogeneity in the regulation of T cell IL 2 secretion . ^^^ In this report , the co stimulatory signals provided by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) were compared to CD 28 ligation by mAb . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , the levels of CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) antigen expression were significantly higher on these T cells and alveolar macrophages in the BALF of patients . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , IL 10 led to the down regulation of various surface antigens , especially of CD 86 and CD 54 , whereas TNF alpha and TRAP enhanced the expression of MHC class 1 and 2 antigens and of the accessory molecules CD 40 , CD 54 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Functional analysis of CD83+ cells revealed that MHC class 2 , CD11a , CD 40 and CD 86 played functionally dominant roles , whereas CD 80 contributed minimally to the specialized costimulatory activity of these potent APC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Highly purified cell preparations of mLC migrating from human epidermis expressed high amounts of major histocompatibility complex ( MHC ) class 1 and 2 antigens and of the accessory molecules CD 40 , CD 80 and CD 86 , indicative of the phenotype of potent immunostimulatory cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) : potential targets for immunotherapy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recognition of antigen / MHC complexes by the T cell receptor delivers the first signal , while a second signal , delivered by the cell surface receptors CD 80 and / or CD 86 binding to the T cell surface molecule CD 28 , has been shown to be effective for the initiation of effective T cell responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Among various molecules known to provide costimulatory function , the expression of CD 54 ( ICAM 1 ) and CD11a / CD18 ( LFA 1 ) on MAIDS B cells was increased , whereas that of CD 2 , heat stable Ag ( CD 24 ) , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) was unchanged from normal . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A major co stimulatory pathway involves crosslinking the CD 28 molecule on T cells by its ligands CD 80 or CD 86 expressed on antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The functional maturation of dendritic cells ( DC ) and other antigen presenting cells is believed to reflect the upregulation of cell surface major histocompatibility complex ( MHC ) class 2 and other T cell co stimulatory molecules , especially the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
As GC cells , these cells also expressed CD 71 , CD 80 ( B7 . 1 ) , and CD 86 ( B7 . 2 ) , but not CD 24 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential effects of CTLA 4 substitutions on the binding of human CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ CTLA 4 expressed on activated T cells binds to CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules present on APC with high avidity and appears to deliver a negative regulatory signal to the T cell . ^^^ We have investigated the kinetics of CTLA 4 binding to CD 80 and CD 86 , together with the effects of selected CTLA 4 mutations on binding activity . ^^^ The dissociation constants ( Kd ) for binding of CTLA 4 Ig to CD 80 and CD 86 transfectants were 8 . 1 and 6 . 7 nM , respectively . ^^^ Surface plasmon resonance was used to determine kinetic parameters of CTLA 4 Ig binding to CD 80 Ig and CD 86 Ig fusion proteins and revealed enhanced association ( ka ) and dissociation ( kd ) rate constants for CD 86 Ig compared with CD 80 Ig . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Malignant B lymphocytes from non Hodgkin ' s lymphoma induce allogeneic proliferative and cytotoxic T cell responses in primary mixed lymphocyte cultures : an important role of co stimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) in stimulation by tumor cells . ^^^ To investigate the role of co stimulatory molecules CD 80 and CD 86 for the stimulatory capacities of B cells , the expression of both molecules was analyzed before and during the MLR . ^^^ Most fresh malignant B cells were negative for CD 80 and CD 86 , whereas co cultured B cells expressed high levels of both molecules . ^^^ This expression was crucial for T cell proliferation , since monoclonal antibodies directed against CD 80 and CD 86 completely abrogated the MLR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Effect of CD 80 and CD 86 on T cell cytokine production . ^^^ Conjugation of the T cell receptor ( TCR ) with antigen / MHC proteins must be accompanied by conjugation of T cell counterreceptors ( CD 28 or CTLA 4 ) with costimulatory molecules CD 80 or CD 86 ( B 7 1 or B 7 2 ) on antigen presenting cells ( APC ) to avert T cell anergy , and to provide essential signals for T cell activation and cytokine production . ^^^ To determine the involvement of CD 80 and CD 86 in costimulation of T cell cytokine production , T cells were incubated with peritoneal exudate macrophages , which express CD 80 and CD 86 , and stimulated in vitro for 48 or 72 hrs with anti CD 3 in the presence or absence of blocking antibody to CD 80 or CD 86 . ^^^ Production of T cell IL 2 , IL 4 , and IL 5 was depressed in the presence of anti CD 86 but not anti CD 80 . ^^^ Production of IFN gamma was significantly blocked by either anti CD 80 and anti CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The results suggest that potent co stimulatory pathways can be induced by cellular interactions between a T lymphoma , RMA S and T cells , not involving gp 39 , CD 40 , CD 70 , HSA , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fluorescence activated cell sorter analysis revealed that no staining for either CD 80 or CD 86 was observed in freshly isolated Langerhans cells but that both CD 80 and CD 86 were expressed on cultured Langerhans cells . ^^^ Analysis of polymerase chain reaction demonstrated that both CD 80 and CD 86 were specifically amplified from purified cultured and freshly isolated Langerhans cells but not from Langerhans cell depleted epidermal cells , indicating that both CD 80 and CD 86 genes were expressed by Langerhans cells . ^^^ A monoclonal antibody against CD 86 caused 81 % inhibition in contrast with 29 % inhibition produced by anti CD 80 monoclonal antibody . ^^^ This inhibitory effect was enhanced to 85 . 3 % inhibition when a combination of anti CD 86 and anti CD 80 was administered . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Main costimulatory signals are provided to T cells by B 7 antigens ( CD 80 and CD 86 , expressed on antigen presenting cells ( APC ) ) upon interaction with its receptor , the CD 28 molecule expressed on T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) co stimulatory molecules have been reported to activate Th1 / Th2 development pathways differentially . ^^^ Based on the recently reported effect of interferon ( IFN ) gamma and interleukin ( IL ) 10 on the expression of CD 80 and CD 86 by LC , we examined the effects of these cytokines on the expression of CD 54 ( intercellular adhesion molecule 1 ) and CD 40 in addition to CD 80 and CD 86 on LC , and correlated the expression of each co stimulatory molecule with antigen presentation for a Th 1 clone by cultured LC ( cLC ) treated with these cytokines . ^^^ In addition , among the antibodies for CD 80 , CD 86 , CD 28 or CD 40 , the suppression of the Th 1 clone stimulation by LC was found to occur only with anti CD 80 and anti CD 28 antibodies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interactions of CD 80 and CD 86 with CD 28 and CTLA 4 . ^^^ CD 80 and CD 86 are cell surface glycoproteins expressed on a variety of professional APCs . ^^^ These results suggested that other parts of the proteins are important in determining binding , so a series of modified CD 80 and CD 86 molecules were constructed in an attempt to identify other binding determinants . ^^^ Insertion of two residues between the two Ig domains of CD 80 resulted in decreased affinity for CTLA 4 , but a similar mutation in CD 86 was without effect . ^^^ We also identified another asymmetry between CD 80 and CD 86 in that the 5 domain of CD 86 but not that of CD 80 is sufficient for CTLA 4 binding . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Because rheumatoid synovium is characterized by an ongoing immune response , the expression and function of CD 80 , CD 86 , and other markers of DC differentiation by rheumatoid arthritis synovial APC were examined . ^^^ Whereas CD 80 expression by rheumatoid SF DC and monocytes was minimal , CD 86 was expressed by a subset of SF monocytes and by CMRF 44+SF DC . ^^^ CD 80 was expressed diffusely and at low levels by rheumatoid synovial tissue cells , whereas CD 86 was expressed by perivascular HLA DR+HLA DQ+CD80+CMRF 44+ DC , and by some CD14+ monocytes . ^^^ Anti CD 86 mAb and CTLA 4 Ig , but not anti CD 80 mAb , inhibited the MLR stimulated by SF DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression and function of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on human epidermal Langerhans cells . ^^^ We analyzed the expression and function of the two ligands for CD 28 , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , on human Langerhans cells ( LC ) , the antigen presenting cells from epidermis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
YT mediates lysis of CD80+ / CD86+ B lymphoblastoid cell lines and the murine mastocytoma p 815 transfected with CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast to macrophages , all three DC populations expressed the DC marker CD 83 , as well as highlevels of MHC molecules and the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The present study compares the mitogen activated protein ( MAP ) kinase responses in T cells activated with the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 / B70 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential expression of the costimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) in rheumatoid synovial tissue . ^^^ The latter may be provided by B7 . 1 ( CD 80 ) or B7 . 2 ( CD 86 ) on APC interacting with CD 28 on T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To determine if KS tumor cells can stimulate resting T cell proliferation , we asked whether the tumor cells express the critically important T cell costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We analyzed the expression and function of the co stimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) during contact sensitivity reactions induced by the hapten 2 , 4 dinitrofluorobenzene ( DNFB ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD1a+ cells differed from CD14+ cells by ( 1 ) dendritic morphology , ( 2 ) higher expression of CD1a , CD1c , CD 4 , CD 40 , adhesion molecules ( CD11c , CD 54 , CD 58 ) , major histocompatibility complex ( MHC ) class 2 molecules and CD 28 ligands ( CD 80 and CD 86 ) , ( 3 ) lack of Fc receptor FcgammaRI ( CD 64 ) and complement receptor CR 1 ( CD 35 ) expression , and ( 4 ) stronger induction of allogeneic T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Decreased inducible expression of CD 80 and CD 86 in human monocytes after ultraviolet B irradiation : its involvement in inactivation of allogenecity . ^^^ Costimulation by the CD 28 ligand CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) plays an important role during T cell proliferation by augmenting synthesis of interleukin 2 ( IL 2 ) and other cytokines . ^^^ In this study , we demonstrated induced expression of both CD 80 and CD 86 during allogeneic MLR , though human freshly isolated monocytes express CD 86 constitutively with a much lower level of CD 80 . ^^^ A monoclonal antibody ( MoAb ) against CD 86 , but not CD 80 , efficiently inhibited allogeneic T cell proliferative responses stimulated with highly purified monocytes . ^^^ The surface expression of CD 80 , CD 86 , CD 54 , and HLA DR on monocytes was further augmented by interferon ( IFN ) gamma ; this cytokine induced expression was dose dependently reduced by UV B irradiation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Specific acceptance of cardiac allografts after treatment with antibodies to CD 80 and CD 86 in mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expansion of TIL was partially blocked by the addition of CTLA 4 Ig , which is an inhibitor of costimulatory molecules such as CD 80 and CD 86 , and was almost blocked by the addition of anti ( Fc receptor gamma 2 ) mAb . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , the combination of GM CSF and TNF alpha supports quantitative differentiation into dendritic cells , lacking c fms , CD 14 , and other macrophage properties , and expressing HLA DR , CD1a , CD 83 , CD 80 , CD 86 , and potent allostimulatory activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Opposing effects of CTLA 4 Ig and anti CD 80 ( B 7 1 ) plus anti CD 86 ( B 7 2 ) on experimental allergic encephalomyelitis . ^^^ The roles of the B 7 receptors , CD 80 and CD 86 , during actively induced experimental allergic encephalomyelitis were examined with specific monoclonal antibodies and CTLA 4 Ig . ^^^ Surprisingly , combined anti CD 80 + anti CD 86 monoclonal antibody injection on day 2 resulted in marked exacerbation of disease . ^^^ These results suggest distinct roles for CD 80 and CD 86 in the initiation of EAE , resulting in the diverse clinical outcomes observed in this model of EAE . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Neisserial porins increased the surface expression of the costimulatory ligand B 7 2 ( CD 86 ) but did not affect the expression of B 7 1 ( CD 80 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cross linking of CD 40 on directly isolated DC with mouse CD40L trimer ( mCD40LT ) markedly augmented CD 80 and CD 86 up regulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Primary and cultured H RS cells frequently overexpress intercellular adhesion molecule 1 ( ICAM 1 / CD54 ) , BB 1 ( B 7 1 / CD80 ) and B70 / B7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Two molecules capable of providing a costimulatory signal , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , have been shown to augment the immunogenicity of whole tumor cell vaccines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The interaction between CD 28 on T cells with CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on APCs is considered to be of critical importance for primary T cell activation both in vivo and in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The differential role of CD 86 and CD 80 co stimulatory molecules in the induction and the effector phases of contact hypersensitivity . ^^^ We examined the distinct role between CD 80 and CD 86 on hapten induced CH in both an induction and an effector phase . ^^^ Intraperitoneal injection of mAb against CD 86 , but not CD 80 , 2 h before sensitization with epicutaneous application of dinitrofluorobenzene led to an almost complete inhibition of ear swelling , and histologically a marked reduction of edema , inflammatory polymorphonuclear cells and lymphocyte infiltration in the dermis . ^^^ In contrast , the administration with either anti CD 80 or CD 86 mAb 2 h before challenge partially inhibited CH reactions and a combination of both mAb did not improve the inhibitory effect . ^^^ Although Langerhans cells ( LC ) expressing MHC class 2 were observed in both the epidermis and dermis 24 h after primary sensitization , CD 86 ( + ) LC were observed only in the subepidermal regions and CD 80 bearing cells were not detected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
T lymphocyte receptor CTLA 4 binds costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) with high avidity and negatively regulates T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Analysis of the site of interaction of CD 28 with its counter receptors CD 80 and CD 86 and correlation with function . ^^^ Both receptors associate with the counter receptors CD 80 and CD 86 , but the avidity of interaction for CD 28 is about 20 fold lower than for CTLA 4 . ^^^ The conservative F substitution of Y 26 in the CDR 1 like region also reduced binding to CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On the other hand , activation of CD8+ cells by allogeneic MHC class 1 on EBV transformed B cells , which express two different CD 28 ligands , CD 80 and CD 86 , also proceeded very efficiently ( proliferation , cytotoxic activity and CD 25 expression ) , but was either not , or only partially , blocked by anti CD 80 and anti CD 86 MoAb or CTLA 4Ig . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The binding of CD 40 ligand on activated T cells to CD 40 on resting B cells induces the expression of costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression and function of CD 80 and CD 86 costimulator molecules on synovial dendritic cells in chronic arthritis . ^^^ To examine CD 86 expression on dendritic cells isolated from the synovial fluid ( SFDC ) of patients with chronic arthritis , and to determine the importance of both CD 80 and CD 86 molecules in SFDC T lymphocyte interactions . ^^^ The costimulator activity of the SFDC CD 80 and CD 86 molecules was determined by allogeneic mixed lymphocyte reaction ( MLR ) . ^^^ CD 80 and CD 86 induction on SFDC during in vitro culture was also examined . ^^^ Fresh SFDC either lacked or showed very weak surface expression of CD 86 molecules ( as shown previously for CD 80 ) , yet contained CD 86 mRNA . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present report , we show that pre B cell ALL express alloantigen and adhesion molecules but uniformly lack B 7 1 ( CD 80 ) and only a subset express B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The HSV IPC expression of CD 13 , CD 33 and Fc epsilon RI were weak but significant , while no CD 5 , CD11b , CD 16 , CD 64 , CD 80 or CD 86 were detected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
T cell costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are expressed in human microglia but not in astrocytes in culture . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 molecules ( CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) ) on APCs provide costimulatory signals for T cell proliferation . ^^^ When resting T cells were cocultured with CHO cells expressing B 7 1a molecules in the presence of PMA / ionomycin , T cell proliferation was not detected , while CHO cells either expressing CD 80 or CD 86 could promote the proliferation of resting T cells . in contrast to resting T cells , CHO cells expressing B 7 1a could support the proliferation of activated T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory effects of T cell proliferation during infection with human T lymphotropic virus types 1 and 2 are mediated through CD 80 and CD 86 ligands . ^^^ The modulation of expression of CD 80 and CD 86 on T cells following infection with human T lymphotropic virus ( HTLV ) I / II and its functional importance in T T cell interactions was examined . ^^^ Infection with HTLV I / II leads to constitutive expression of CD 80 and CD 86 , concomitant to down modulation of CD 28 on T cells . ^^^ The CD80 / CD86+ HTLV infected T cells stimulated proliferation of allogeneic and autologous resting T cells , which could be specifically blocked by a soluble CTLA 4Ig chimeric protein , anti CD 80 or anti CD 86 , but not by anti CD 54 . ^^^ It was necessary to inhibit interaction with both ligands ( CD 80 and CD 86 ) to optimally block HTLV mediated proliferation of allogeneic and autologous resting T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both precursor subsets mature at day 12 14 into DC with typical morphology and phenotype ( CD 80 , CD 83 , CD 86 , CD 58 , high HLA class 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , CD40L is the most potent stimulus in upregulating the expression of ICAM 1 , CD 80 , and CD 86 molecules on DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 , a CD 28 homologue expressed on activated T cells , binds with high affinity to the CD 28 ligands , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The gp 120 induced reduction in the proliferative responses of PBMC was at least partially reversed by the addition of IL 2 , anti CD 28 MAb , or transfectants expressing CD 80 , CD 86 , or CD 40 but not with exogenous IL 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Increased expression of CD 80 , CD 86 and CD 70 on T cells from HIV infected individuals upon activation in vitro : regulation by CD4+ T cells . ^^^ These ligands , CD 80 , CD 86 and CD 70 , are also expressed to some extent on activated T cells . ^^^ On the other hand , T cell stimulation in vitro induced high CD 80 , CD 86 and CD 70 expression on T cells from HIV infected individuals . ^^^ Indeed , high expression levels of CD 80 , CD 86 and CD 70 were found on activated CD8+ T cells from HIV individuals cultured in the absence of CD4+ T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Nef down regulated IL 2 , but not IL 4 produced upon induction by combinations of mitogens that mimicked TcR signals together with CD 28 mAb or CD 28 ' s natural ligand ( CD 80 and CD 86 ) . ^^^ However , the co signals provided by CD 28 to up regulate IL 2 induction were unaffected by Nef , since IL 2 produced by nef transfected cells was proportionally enhanced to the same extent as that of control cells , either upon stimulation by the CD 28 mAb or CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The B 7 receptors , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , on the Ag presenting cell ( APC ) , interact with T cell CD 28 or CTLA 4 to deliver a costimulatory signal , which is particularly important for Th 1 activation . ^^^ Using a soluble fusion protein ligand for the B 7 receptors , as well as specific monoclonal antibodies specific for either CD 80 or CD 86 , it has been demonstrated that B 7 costimulation plays a prominent role in determining clinical disease outcome in EAE . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interestingly , DC express a functional CD 40 , which triggering upregulates expression of CD 80 and CD 86 and induces cytokine production , indicating a reciprocal talk between DC and T cells during the course of antigen presentation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Requirement of CD 80 and CD 86 molecules for antigen presentation by eosinophils . ^^^ Furthermore , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) were expressed on the surfaces of untreated eosinophils . ^^^ Moreover , anti CD 80 or anti CD 86 MoAbs blocked proliferative responses of primed lymph node T cells and thymus T cells to SEB , and also blocked responses of primed lymph node T cells to OVA . ^^^ Thus , CD 80 and CD 86 play an important role in stimulation of T cells by eosinophils . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) provide costimulation through CD 28 , their ligand on the T cell . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In order to stimulate T cells efficiently , SE B cells had to be exposed to anti mu antibody , a treatment which induced expression of activation markers such as CD 80 , CD 86 , CD 69 and CD 39 , usually absent in resting SE B cells . ^^^ CD 80 and CD 86 molecules expressed by SE B cells participated in the chain of events required to promote the proliferation of allogeneic T cells as demonstrated by inhibition tests with the appropriate mAb . ^^^ The expression of CD 80 and CD 86 by anti mu treated SE B cells was not , however , the sole explanation for their good antigen presenting capacities since the exposure of FM B cells to anti mu antibody also induced expression of these surface structures . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Two distinct signals are required to activate T cells : an Ag specific signal and a costimulatory signal mediated primarily by B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) through interactions with CD 28 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Islet infiltrating t lymphocytes in insulin dependent diabetic patients express CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ While no biopsy revealed any islet cell specific CD 80 or CD 86 expression , biopsies from six of the nine patients with insulitis revealed both CD 80 and CD 86 expression on the islet infiltrating cells . ^^^ Of the CD 3 positive cells , 19 . 4 % expressed CD 80 and 21 . 7 % expressed CD 86 . ^^^ CD 80 and CD 86 positive cells were similarly distributed throughout the inflamed islets . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In particular , we discuss here whether the type of antigen presenting cells ( APCs ) and the distinct expression of important adhesion molecules like CD 80 and CD 86 may influence the generation of CD4+ CTLs . ^^^ The level of CD 80 , but not of CD 86 , present on the APCs appears to be crucial for the induction of CD4+ CTLs . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
LC were CD1a+2 DR+2 , CD23+ , CD36+ , CD 80 , CD 86 , and CD 25 , while DC were CD1a+ / DR+3 , CD 23 , CD 36 , CD80+ , CD86+2 , and CD25+ , CD 40 and CD 32 were moderately expressed and nearly unchanged on maturation , in contrast to monocyte derived DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Infusion of anti B7 . 1 ( CD 80 ) and anti B7 . 2 ( CD 86 ) monoclonal antibodies inhibits murine graft versus host disease lethality in part via direct effects on CD4+ and CD8+ T cells . ^^^ We have found that the infusion of anti B7 . 1 ( CD 80 ) + anti B7 . 2 ( CD 86 ) mAb is effective in eliminating GVHD lethality induced by either CD8+ or CD4+ T cells . ^^^ Although anti CD 80 or anti CD 86 mAb individually were ineffective in preventing CD8+ T cell GVHD lethality , each mAb was partially effective in CD4+ T cell mediated GVHD . ^^^ These studies demonstrate that anti CD 80 + anti CD 86 mAb infusion is effective in preventing GVHD lethality by inhibiting donor CD4+ or CD8+ T cell expansion and provide the first evidence that CD 80 expression on donor T cells is critical for optimal GVHD lethality . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They bound human and murine CTLA 4 immunoglobulin ( CTLA 4 Ig ) fusion proteins indicating expression of CD 80 and or CD 86 . ^^^ The findings indicate that CD 80 and or CD 86 are involved in the stimulation of CD4+ and CD8+ alpha beta TCR+ T cells but not in the stimulation of either of the two gamma delta TCR+ populations . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Thus , CD 18 mAb inhibited the apoptotic response to IL 2 deprivation , whereas mAb against other adhesion molecules ( CD 28 , CD 29 , CD49d , CD 80 , CD 86 ) did not . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulation of human T cells by pCD 86 in both systems is as effective as costimulation by human CD 80 or CD 86 , and can be blocked by human CTLA4Ig . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
There were no differences in expression of CD 80 and CD 86 surface molecules by dendritic cells that were either mock stimulated or stimulated with HIV 1 or HSV for 24 h . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have shown previously that granulocyte macrophage colony stimulating factor stimulated mouse bone marrow derived MHC class II+ dendritic cell ( DC ) progenitors that are deficient in cell surface expression of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) can induce alloantigen specific T cell anergy in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Appearance of cells expressing CD 80 and CD 86 costimulatory antigens in the pancreas of nonobese diabetic mice . ^^^ To evaluate the role of the CD 80 and CD 86 antigens in the development of insulin dependent diabetes mellitus , expression of these antigens in the pancreas of normal and nonobese diabetic mice was studied . ^^^ The pancreata of normal BALB / c mice and young nonobese diabetic mice contained no CD 80 or CD 86 positive cells . ^^^ CD 80 and CD 86 positive cells appeared in the pancreas of nonobese diabetic mice by 6 weeks of age . ^^^ The morphology of the CD 80 and CD 86 positive cells was heterogeneous . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
T lymphocyte receptors CD 28 and CTLA 4 bind costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on antigen presenting cells and regulate T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The testes of 4 week old NOD mice or normal BALB / c mice and the testis of 17 21 week old BALB / c mice contained no CD 80 or CD 86 expressing cells . ^^^ The CD80+ and CD86+ cells were often located adjacent to the vessel walls where a leukocyte not expressing CD 80 or CD 86 had attached to the endothelium . ^^^ The CD 80 and CD 86 antigens could not be observed in the same cells as judged from stainings in parallel sections . ^^^ The results suggest that T lymphocytes can not be activated locally in the testis of BALB / c and young NOD mice because of the absence of the necessary CD 28 ligands , CD 80 and CD 86 , from the APCs and because of the suppression of T lymphocytes by the testicular products . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that microglia with and without alloAgP activity expressed similar levels of major histocompatibility complex class 1 molecules ; however , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expression was primarily restricted to the alloAgP cell lines . ^^^ Monoclonal antibody ( Mab ) to CD 80 only partially blocked the proliferative response of allogeneic CD8+ T cells cocultured with the presenting cell lines , whereas Mab to CD 86 completely inhibited the response , indicating a significant role for this molecule in T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CD28 / CTLA 4 ligands , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , provide a co stimulatory signal necessary for optimal T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of co stimulatory molecules CD 80 and CD 86 was suggested by the ability of mast cells to activate purified lymph node derived T cells . ^^^ The regulatory mechanisms of APC function by GM CSF and IFN gamma were investigated by measuring CD 80 and CD 86 transcripts in mast cells . ^^^ GM CSF treated must cells showed a strong increase in the expression of both CD 80 and CD 86 transcripts , whereas in IFN gamma treated mast cells , this expression was completely abrogated . ^^^ Thus , up and down regulation of CD 80 and CD 86 expression by GM CSF and IFN gamma is directly correlated to the APC function . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 co stimulatory regimes differ in their dependence on phosphatidylinositol 3 kinase : common co signals induced by CD 80 and CD 86 . ^^^ CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) ligation of CD 28 provide co stimulatory signals required for optimal lymphokine production in response to TCR zeta CD 3 ligation . ^^^ In this study , we have investigated whether CD 28 associated Pl 3 kinase is required for CD 80 and CD 86 co stimulation , as well as in co signaling that involves different primary signals ( i . e . ^^^ In the presence of anti CD 3 , ligation of CD 28 by both CD 80 and CD 86 was found to induce Pl 3 kinase recruitment and IL 2 production . ^^^ CD 80 and CD 86 therefore share a common signaling pathway leading to IL 2 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immunophenotypical studies have demonstrated frequent expression of lymphoid `` activation markers ' including CD 15 , CD 25 , CD 30 , CD 40 , CD 54 , CD 70 , CD 71 , CD 80 , CD 86 and MHC class 2 and less frequent expression of T or B cell associated antigens by the neoplastic H RS cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cause of such an anergic reaction is not clear since RS cells express HLA class 2 as well as the co stimulator molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 80 and CD 86 was enhanced by adding CD40L and IL 4 , and the DC showed stimulatory activity in MLC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The costimulatory molecules CD 80 and CD 86 affect the differentiation of Th 1 and Th 2 subsets in experimental allergic encephalomyelitis , an autoimmune disorder . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory function and expression of CD 40 ligand , CD 80 , and CD 86 in vascularized murine cardiac allograft rejection . ^^^ Alternatively , the ability of CD 40 stimulation to induce CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expression on antigen presenting cells ( APCs ) has led to the hypothesis that the role of CD 40 CD40L interactions in transplant rejection might be indirect , i . e . , to promote the costimulatory capacity of APCs . ^^^ Anti CD40L mAb has no detectable effect on CD 86 up regulation , but almost completely abolishes induction of CD 80 . ^^^ However , animals treated with anti CD 80 mAb or with a mutated form of CTLA4Ig ( which does not bind to CD 86 ) rejected their cardiac allografts , indicating that blockade of CD 80 alone does not mediate the graft prolonging effects of anti CD40L mAb . ^^^ These data support the notion that the role of CD 40 CD40L in transplant rejection is not solely to promote CD 80 or CD 86 expression , but rather that this pathway can directly and independently costimulate T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blocking of both B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) was required in order to inhibit cytokine production , indicating that either molecule could act alone . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interaction of the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules on antigen presenting cells with the receptors CD 28 and CTLA 4 on T cells generates signals important in the regulation of immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The aim of this study was to investigate the mechanisms by which B 7 related costimulatory molecules ( CD 80 , CD 86 ) increase T cell responsiveness to extracellular ligands . ^^^ Indeed , experiments performed with purified subpopulations of antigen presenting cells and using B 7 transfected cell lines indicated that increased levels of CD 80 and / or CD 86 cell surface expression is associated with a faster kinetics of IL 2 production in response to SEB . ^^^ Accordingly , blocking of CD 80 or CD 86 derived signals by specific monoclonal antibodies led to a slower kinetics of IL 2 production in response to SEB . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , FACS analysis on day 7 PI showed that HIV did not affect differentiation of DC that survived CPE : apart from CD 4 downmodulation related to HIV production , overall expression of CD 40 , CD 80 , and CD 86 costimulatory molecules , and of HLA DR , was unchanged relative to controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Purified BDCs from cholestatic C57BL / 6 ( H 2b ) mice express not only the major histocompatibility complex ( MHC ) class 1 and class 2 antigens but also the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cultured DC also expressed high levels of class 1 and class 2 major histocompatibility complex , together with CD11c , DEC 205 , CD 80 , and CD 86 , markers characteristic of mature DC in general . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulation with this mAb induced a similar level of proliferative responses of both B 1 and B 2 cells , as well as an increase in expression of cell surface molecules 1 A , CD 54 , CD 23 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Effect of CD 80 and CD 86 blockade and anti interleukin 12 treatment on mouse acute graft versus host disease . ^^^ We investigated the efficacy of a combination of anti CD 80 and CD 86 ( CD 80 + 86 ) monoclonal antibodies ( mAb ) , anti interleukin ( IL ) 12 mAb , or both , for prophylaxis in a mouse acute graft versus host disease ( GVHD ) model . ^^^ The treatment with a combination of anti CD 80 + 86 mAb efficiently reduced the lethality of GVHD , whereas mAb against either CD 80 or CD 86 alone had an effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cells showed morphologic and immunophenotypical features typical of DCs , including expression of major histocompatibility complex ( MHC ) class 1 and 2 molecules , CD1a , CD 80 , CD 86 , CD 13 , CD 33 , CD 40 , and CD 54 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules was closely regulated during development of the disease . ^^^ Expression of both CD 80 and CD 86 increased on cells in pancreatic lymph nodes in STZ treated C57BL / KsJ mice . ^^^ In wild type animals , treatment with mAb against CD 86 prevented , whereas treatment with mAb against CD 80 exacerbated , insulitis and hyperglycemia , indicating that mAbs against these molecules differentially affect development of disease . ^^^ CD 80 and CD 86 molecules , the CD28 / CTLA4 ligands , may have different roles in regulation of the disease and affect T cell function at steps beyond differentiation into mature phenotypes . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 ( B 7 1 and B 7 2 ) are the ligands on antigen presenting cells ( APCs ) which bind CD 28 and deliver the costimulatory signals necessary for T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of costimulatory molecules CD 80 and CD 86 in human carcinoma cell lines : its regulation by interferon gamma and interleukin 10 . ^^^ The costimulatory signal through CD 80 or CD 86 to its counterreceptor CD 28 or CTLA 4 on T cells has been shown to play an important role in the induction of T cell mediated immunity against tumors . ^^^ In the present study , we examined the expression of CD 80 and CD 86 in the cell lines derived from human gastric , esophageal and colorectal carcinomas at the mRNA level , by means of a reverse transcriptase / polymerase chain reaction analysis and , for their surface expression , using a flow cytometric analysis with monoclonal antibodies ( mAb ) . ^^^ The expression of mRNA for CD 80 or CD 86 was detected in all 18 cell lines tested , except for CD 86 on one cell line . ^^^ The cells from 13 ( 72 % ) or 12 ( 67 % ) of these cell lines expressed the surface CD 80 or CD 86 molecule , detected with the respective mAb . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 , CD 86 and CD 40 provide accessory signals in a multiple step T cell activation model . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The antibody BB 1 did not inhibit proliferation and cytokine secretion , nor did the anti CD 86 clone IT2 . 2 . ^^^ Differential expression and function of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on human peripheral blood monocytes . ^^^ Recent studies demonstrated the existence of at least two ligands on accessory cells , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ In this study we demonstrate that , although CD 80 and CD 86 are both expressed on monocytes , they seem to have different functions . ^^^ Freshly isolated monocytes express CD 86 but are CD 80 negative . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypic analysis showed that TFC , after incubation with interferon gamma , do not express the costimulatory molecules B 7 1 ( CD 80 ) and 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although the medullary foci lack central organization , the expression in these regions of CD 80 , CD 86 and CD 40 , as well as the clustering of dendritic cells , is similar to that observed in medullae of wild type mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To investigate expression of the co stimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on tumor associated dendritic cells ( TADCs ) , cryosections from basal cell carcinomas were immunostained . ^^^ In basal cell carcinomas , only 1 to 2 % of intratumor and 5 to 10 % of peritumor APCs expressed CD 80 or CD 86 . ^^^ In contrast , biopsies of immunological / inflammatory dermatoses revealed that 38 to 73 % of APCs expressed CD 80 and CD 86 . ^^^ When analyzed by flow cytometry , TADCs expressed high levels of HLA DR , but only 5 to 10 % co expressed CD 80 or CD 86 . ^^^ A 3 day culture in granulocyte / macrophage colony stimulating factor containing medium partially reconstituted the TADC expression of CD 80 and CD 86 as well as their immunostimulatory capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The interaction of CD 28 with B 7 molecules ( CD 80 or CD 86 ) is an essential second signal for both the activation of CD4+ T cells through the T cell receptor and the prevention of anergy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To study the functions of B 7 molecules on T cells , we transfected murine B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) cDNAs into the EL 4 T cell thymoma cell line and examined the transfectants for their ability to costimulate T cell proliferation in vitro and to induce antitumor immunity in vivo . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The structurally related T cell surface molecules CD 28 and CTLA 4 interact with cell surface ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on antigen presenting cells ( APC ) and modulate T cell antigen recognition . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition to an antigen specific signal , T cell activation requires an antigen independent costimulatory signal provided by interaction of CD 28 with B 7 ( CD 80 and CD 86 ) on the APC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Role of costimulators in T cell differentiation : studies using antigen presenting cells lacking expression of CD 80 or CD 86 . ^^^ For T cells to be optimally activated , recognition of Ag / MHC complexes by the TCR must be accompanied by a second , costimulatory signal that can be provided efficiently by the related costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ Recently , CD 80 and CD 86 have been implicated as differential determinants of Th 1 vs Th 2 type cytokine profiles . ^^^ To investigate the role of CD 80 and CD 86 in Th subset differentiation , we have examined the cytokine profiles induced in TCR transgenic T cells stimulated by peptide in association with splenic APCs obtained from knockout mice that selectively lack expression of either the CD 80 or the CD 86 molecule . ^^^ Our data suggest that CD 86 , and to a lesser extent CD 80 , can make significant contributions to the production of both IL 4 and IFN gamma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CTA 1 DD , as well as CT , up regulated expression of the CD 80 and CD 86 molecules on the targeted B cells , indicating that enhanced T cell costimulation may be responsible for the adjuvant effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Since the expression of either B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) on APC is required for the activation of T cells , it was important to establish human gastric epithelial cells expressed those surface ligands . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To investigate whether the immunosuppressing capacity of alpha MSH is associated with the up regulation of MC 1 , its effect on the expression of costimulatory molecules ( CD 86 and CD 80 ) on human monocytes was investigated . alpha MSH significantly inhibited the expression of CD 86 on LPS treated monocytes , which exhibited a high density of MC 1 , whereas CD 80 expression was not altered . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Prevention of primary T cell responses by immunotoxins directed against CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
For flow cytometric analysis , epidermal cell ( EC ) suspensions were prepared and double labeled with monoclonal antibodies against CD1a or HLA DR , and B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , ICAM 1 ( CD 54 ) , ICAM 3 ( CD 50 ) , LFA 3 ( CD 58 ) , E cadherin , or integrin beta 4 ( CD 104 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD4+ T cells costimulated with the A6H mAb upregulated CD 80 , CD 86 , CD 71 , interleukin 2 ( IL 2 ) R alpha , IL 2R beta and IL 2R gamma , while no corresponding up regulation of these cell surface molecules was seen in CD8+ T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Therefore , we studied proliferation and cytokine production in highly purified CD8+ and CD4+ T cells from HIV infected and HIV subjects , induced by co activation via cell bound CD 80 , CD 86 and CD 40 or by allo activation . ^^^ Anti CD 3 combined with CD 80 or CD 86 induced a mixed Th1 / Th2 type cytokine profile in both CD4+ and CD8+ T cells from controls , whereas anti CD 3 plus CD 40 induced only low levels of Th 2 type cytokines and no interferon gamma ( IFN gamma ) in CD4+ T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 40 triggering of chronic lymphocytic leukemia B cells results in efficient alloantigen presentation and cytotoxic T lymphocyte induction by up regulation of CD 80 and CD 86 costimulatory molecules . ^^^ Freshly collected chronic lymphocytic leukemia B cells ( B CLL cells ) are known to be inefficient at stimulating allogeneic T cells , and to lack significant expression of B 7 ( CD 80 and CD 86 ) costimulatory molecules . ^^^ B CLL cells cocultured with human CD 40 ligand expressing mouse fibroblasts rapidly up regulated CD 54 and CD 58 adhesion molecules and B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulatory molecules , and acquired a strong stimulatory capacity towards CD4+ as well as isolated CD8+ allogeneic T cells . ^^^ Costimulation by both CD 80 and CD 86 proved critical for allogeneic T cell proliferation and CD 25 and HLA DR expression , since these were strongly inhibited by anti CD 80 or anti CD 86 monoclonal antibodies , and completely abrogated by CTLA 4 Ig fusion protein , which blocks both CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Molecular cloning and comparative analysis of the rhesus macaque costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ To facilitate analysis of the role of costimulatory molecules in a nonhuman primate model , we cloned and sequenced the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) costimulatory molecules from rhesus macaques . ^^^ Rhesus CD 80 and CD 86 were highly homologous to their human counterparts , with overall amino acid homologies of greater than 90 % , and were specifically recognized by murine antihuman CD 80 or CD 86 monoclonal antibodies . ^^^ Stable cell lines expressing rhesus CD 80 or CD 86 induced proliferation of suboptimally activated CD4+ T cells and transcription of cytokine mRNA . ^^^ Both CD 80 and CD 86 were able to provide costimulation for interferon gamma and IL 2 synthesis by rhesus CD4+ T cells , but CD 80 costimulation also resulted in synthesis of IL 4 and IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on human hepatocellular carcinoma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The second , costimulatory signal can be provided by cell surface molecules on APCs such as B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , which interact with their counter receptors on T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Lack of CD 28 costimulation through CD 80 and CD 86 molecules on APC might play a causative role in anergy induction , as previously shown with T cell clones . ^^^ Addition of either CTLA 4Ig or blocking anti CD 80 and anti CD 86 mAbs , in combination with cyclosporin A , to cultures of PBMC with soluble Ag consistently resulted in Ag specific unresponsiveness , as evidenced upon antigenic rechallenge . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Inflammatory cytokines IFN gamma plus TNF alpha induce regulated expression of CD 80 ( B 7 1 ) but not CD 86 ( B 7 2 ) on murine fibroblasts . ^^^ Considerable experimental data now suggest that this costimulatory signal is generated predominantly by CD 28 when engaged by its ligands CD 80 ( B 7 1 ) and / or CD 86 ( B 7 2 ) . ^^^ Whether T cell activation is controlled in part by regulated CD 80 and / or CD 86 expression has been incompletely explored . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The inhibitory effect of interleukin ( IL ) 10 on the accessory cell function of AMs and the expression of CD 80 and CD 86 on AMs were also investigated . ^^^ Expression of CD 80 and CD 86 on AMs from these groups did not differ . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , costimulatory proteins on antigen presenting cells , bind to CD 28 on T cells . ^^^ In the experimental autoimmune encephalomyelitis model of MS , CD 80 on antigen presenting cells induces Th 1 cell responses ; CD 86 enhances generation of Th 2 cells . ^^^ Variation in CD 80 and CD 86 expression is likely to influence immune regulation in MS . ^^^ Therapy with IFN beta 1b markedly reduces the number of circulating CD 80 ( + ) B cells and increases CD 86 ( + ) monocyte number . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Isolated IDC displayed a phenotypic profile similar to that of IDC in tonsils and spleen in situ , characterized by high level expression of major histocompatibility complex class 2 , the co stimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) , expression of the late DC maturation marker CD 83 , and no expression of CD1a , CD 13 , or CD 33 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 is a costimulation receptor that binds to the same ligands , CD 80 and CD 86 , as CD 28 with high affinity and is transiently expressed on the cell surface of activated T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Analysis of CD 80 and CD 86 expression on peripheral blood B lymphocytes reveals increased expression of CD 86 in lupus patients . ^^^ We screened peripheral blood mononuclear cells from 13 SLE patients , all having quiescent disease at the time of analysis , 12 allergy patients , and 21 normal subjects for the expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 , B 70 ) on small ( resting ) and large ( activated ) subsets of CD19+ B cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 28 , CTLA 4 , CD 80 , and CD 86 molecules in patients with autoimmune rheumatic diseases : implications for immunotherapy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mitogenic stimulation of T cells reveals differing contributions for B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found by flow cytometry that purified human fibrocytes express each of the known surface components that are required for antigen presentation , including class 2 major histocompatability complex molecules ( HLA DP , DQ , and DR ) , the costimulatory molecules CD 80 and CD 86 , and the adhesion molecules CD11a , CD 54 , and CD 58 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Ligation of CD 28 on T cells with its natural ligands B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) provides a major costimulatory signal for T cells and is of potential importance for tumor rejection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The interaction between CD 28 and its ligands , CD 80 and CD 86 , is crucial for an optimal activation of antigen specific T cells . ^^^ However , the requirement of CD 80 or CD 86 co stimulation in Th 2 cell differentiation and activation is controversial . ^^^ Freshly isolated murine CD4+ and CD8+ T cells were incubated with P 815 transfectants expressing a similar level of either CD 80 or CD 86 in the presence of anti CD 3 mAb . ^^^ Both CD 80 and CD 86 co stimulated the proliferation of CD4+ and CD8+ T cells at comparable time kinetics and magnitude , but CD 86 alone was able to co stimulate IL 4 and especially IL 10 production in CD4+ T cells . ^^^ In typical Th 2 dependent immune responses elicited by Nippostrongylus brasillensis infection , the anti CD 86 mAb treatment but not the anti CD 80 mAb treatment efficiently inhibited antigen specific IgE and IgG 1 production , which was accompanied with the reduced IL 4 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Regulation of T and B cell responses by modulating interactions between CD28 / CTLA4 and their ligands , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CD 28 ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , are expressed on human tonsillar B cells , and their expression is up regulated by IL 4 , IL 13 , and / or an anti CD 40 monoclonal antibody ( mAb ) . ^^^ Conversely , the anti CD 80 and the other anti CD 86 mAbs tested had no effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To examine whether the ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) affect gene expression of Th 2 cytokines and development of Th 2 mediated pathologic tissue reactions , mice were treated with anti B 7 1 or anti B 7 2 at the time of sensitization to footpad inoculation of Schistosoma mansoni eggs or induction of pulmonary granuloma by i . v . injected eggs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are genetically and structurally related molecules expressed on antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential function of CD 80 and CD 86 transfected human melanoma cells in the presence of IL 12 and IFN gamma . ^^^ Introduction of co stimulatory molecules like CD 80 and CD 86 represents a means to augment the immunogenicity of tumor cells and to induce immune responses directed at tumor antigens . ^^^ Here we compared CD 80 and CD 86 transfected human melanoma cells to induce primary immune responses by their capacity to promote proliferation of human allogeneic resting T lymphocytes . ^^^ CD 80 and CD 86 transfected SkMel 63 melanoma cells induced T cell activation to a comparable degree , which was found to be independent of the cell surface density of these co stimulatory molecules . ^^^ Co expression of CD 80 and CD 86 did not result in a synergistic increase in T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , CD 80 and CD 86 plays differential roles for the development of autoimmune disease since helper T cell development is differentially affected by the blockade of either . ^^^ CD 80 or CD 86 in certain conditions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 on T cells and CD 80 and CD 86 on APC are key molecules in the maintenance and breakdown of anergy . ^^^ CTLA 4Ig fusion protein , that binds to both CD 80 and CD 86 with high affinity and thereby prevents interaction of CD80 / CD86 with CD28 / CTLA 4 , prevents or ameliorates several autoimmune disease in experimental animal models , supporting an importance of this pathway in the development of autoimmune diseases . ^^^ However , the studies using specific monoclonal antibodies against CD 80 and CD 86 have shown different outcomes in individual autoimmune models . ^^^ This suggests that the actual regulatory mechanisms of this pathway in autoimmunity is much more complex , because of the existence of two receptors ( CD 28 and CTLA 4 ) and two ligands ( CD 80 and CD 86 ) and the opposite function of CD 28 and CTLA 4 in T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The DC obtained from the cryopreserved PBMC expressed HLA DR , HLA DQ , CD 80 and CD 86 antigens , and stimulated allogenic PBMC to an extent almost identical to that obtained from fresh PBMC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The modulation of CD 80 and CD 86 expression on the HTLV 1 infected cells and its physiological role in the interaction of infected CD4+ T cells with uninfected CD4+ T cells was examined . ^^^ The HTLV 1 infected CD4+ T cell lines established from ATL patients and normal donors by infecting their CD4+ T cells with the virus expressed CD 80 , CD 86 , and HLA DR , and induced a proliferation of autologous and allogenic CD4+ T cells . ^^^ While the CD4+ T cells stimulated with the autologous HTLV 1 infected cells for 7 days expressed CD 80 and CD 86 but not HTLV 1 gene products , they expressed HTLV 1 gag antigen after 4 weeks . ^^^ The interaction of HTLV 1 infected and uninfected CD4+ T cells was profoundly suppressed by a combination of CD 80 and CD 86 monoclonal antibodies . ^^^ These results suggest that the induction of CD 80 and CD 86 on HTLV 1 infected CD4+ T cells participates actively in the generation of the virus infected progenitor cells . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interferon gamma differentially regulates CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) expression on human Langerhans cells . ^^^ CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) have recently been identified in cultured human Langerhans cells ( LCs ) , although their role and regulatory properties remain unclear . ^^^ We present our comparison of the expression of the molecules , mRNAs and the function between CD 80 and CD 86 in human LCs treated by interferon gamma ( IFN gamma ) . ^^^ We examined the regulatory properties of CD 80 and CD 86 expression in human LCs pretreated with IFN gamma . ^^^ Flow cytometric analysis indicated that the mean fluorescence intensity of CD 86 but not CD 80 was enhanced . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blockade of costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) down regulates induction of contact sensitivity by haptenated epidermal cells . ^^^ The hapten , trinitrobenzene sulphonic acid , induced weak B 7 1 ( CD 80 ) and moderate B 7 2 ( CD 86 ) expression on Langerhans cells and mRNA expression of both molecules in organ cultured murine skin . ^^^ When hapten treated epidermal cells were injected into mice after incubation with anti B 7 2 ( CD 86 ) or B 7 1 ( CD 80 ) antibody the resultant contact sensitivity reaction was decreased to less than 50 % of the control reaction , a reduction which was similar to that seen with the anti ICAM 1 and anti LFA 1 antibody induced inhibition of contact sensitivity . ^^^ Anti B 7 1 ( CD 80 ) or anti B 7 2 ( CD 86 ) antibody also inhibited hapten specific lymphocyte proliferation or the allogenic mixed lymphocyte and epidermal cell reaction in vitro , although the inhibitory effect of anti B 7 1 antibody was not as significant as that of anti B 7 2 antibody . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The majority of DC expressed high levels of class 2 MHC , expressed CD11c , and expressed the costimulator molecules CD 80 , CD 86 , and CD 40 ; CD 80 and CD 40 were further up regulated on culture . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On the other hand cells treated in vitro exhibit on the cell surface higher levels of MHC class 2 , of co stimulatory molecules ( CD 80 , CD 86 ) , of adhesion molecules ( CD 44 , intercellular adhesion molecule 1 ) , and acquire expression of the interdigitating DC surface marker NLDC 145 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Characterization of rat CD 80 and CD 86 by molecular cloning and mAb . ^^^ In the present study , we cloned rat CD 80 and CD 86 cDNA from a HTLV 1 transformed rat T cell line , Lewis S 1 , expressing a high level of CTLA 4 Ig binding proteins . ^^^ The predicted CD 80 and CD 86 polypeptides were composed of 321 and 313 amino acids respectively , and exhibited features common to human and mouse B 7 family proteins . ^^^ Both CD 80 and CD 86 mRNAs were abundantly detected in HTLV 1 transformed rat T cell lines but not in a thymic lymphoma cell line . ^^^ To further explore the function of rat CD 80 and CD 86 , we generated cDNA transfectants and anti rat CD 80 ( 3H5 ) and anti rat CD 86 ( 24F ) mAb . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The inhibitory effects of TGF beta 1 on CD4+ cells are not mediated by alterations in APC costimulation since TGF beta 1 did not inhibit the Ag induced expression of MHC class 2 molecules , CD 80 or CD 86 on splenic APC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The costimulatory genes Cd 80 and Cd 86 are linked on mouse chromosome 16 and human chromosome 3 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Hence , the binding of CD 28 on T cells to both CD 80 and CD 86 ligands represents a crucial initial costimulatory step leading to accelerated graft rejection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have developed expression cassettes for cell surface markers CD 80 and CD 86 , two functionally related costimulatory molecules that play an important role in the induction of T cell mediated immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We used a neutralizing antibody to block CTLA 4 interaction with its ligands CD 80 and CD 86 during infection of mice with the nematode , Nippostrongylus brasiliensis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , no correlation is found between the percentage of CD 80 and CD 86 positive AM in these same patients . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mutation of these residues , which are conserved in the CTLA 4 / CD28 family , significantly reduces binding to CD 80 and / or CD 86 , implicating this patch as a ligand binding site . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The heterogeneous expression of CD 80 , CD 86 and other adhesion molecules on leukemia and lymphoma cells and their induction by interferon . ^^^ CD 80 ( B7 / BB 1 , B 7 1 ) and CD 86 ( B 70 , B 7 2 ) take an important role in the interaction between T lymphocytes and antigen presenting cells ( APCs ) as co stimulatory molecules . ^^^ We analyzed the manifestation of adhesion molecules , including CD 80 and CD 86 , on some leukemia and lymphoma cells . ^^^ Constitutive expression of CD 80 and / or CD 86 was frequently observed on B cell leukemia / lymphoma cells , while it is rare on myeloid leukemia cells . ^^^ Our data implies the immunotherapy via CD 80 and CD 86 for patients with hematological malignancies and the possibility to enhance it using interferons . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The interaction of CD 28 with one of the B 7 molecules ( CD 80 and CD 86 ) on professional antigen presenting cells ( APC ) is generally considered as the most important co stimulatory signal for T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential regulation of human , antigen specific Th 1 and Th 2 responses by the B 7 homologues , CD 80 and CD 86 . ^^^ A selectivity of B7 . 1 ( CD 80 ) for promoting Th 1 responses and B7 . 2 ( CD 86 ) for promoting Th 2 responses in the murine system has recently been suggested . ^^^ Neither independent efficacy nor synergy with anti CD 86 was apparent with anti CD 80 treatment ; two different anti CD 80 blocking antibodies yielded identical results . ^^^ Conversely , antigen specific Th 1 and Th 2 clones were insensitive to treatment with either anti CD 80 , anti CD 86 , or a combination of the two . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules are costimulatory molecules that are indispensable to T cell activation by antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cultured human decidual stromal cells express B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) and stimulate allogeneic T cells . ^^^ In the present study , we show by flow cytometry that these cells also express B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , two antigens involved in the costimulation of T cells by antigen presenting cells . ^^^ Using an immunohistochemical technique , we found that in cryostatic sections of early human decidua , CD 80 and CD 86 were expressed mainly by DSC located around the decidual vessels , a location compatible with preDSC rather than fully differentiated DSC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , DCs incubated for 48 h with multivalent sFc gamma RIII expressed increased levels of CD 40 , CD 80 , CD 86 , CD 54 , CD 58 , HLA class 1 and class 2 molecules and decreased levels of CD 23 and CD 32 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
As expected these DC expressed the relevant cell surface molecules including HLA DR , CD 58 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Small percentages of plasma cells expressed weak but detectable levels of HLA DR , HLA DQ , CD 40 , CD 80 , and CD 86 , which could be upregulated by interferon gamma ( IFN gamma ) and tumor necrosis factor alpha . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Characterization of accessory molecules in murine lung dendritic cell function : roles for CD 80 , CD 86 , CD 54 , and CD40L . ^^^ Lung DCs expressed surface molecules typical of DCs from other sites including CD11a , CD 54 , CD 80 , and CD 86 . ^^^ As is true for DCs in other sites , costimulatory molecules including CD 80 , CD 86 , CD40L , CD 2 , CD 54 , and CD11a played important roles in lung DC initiated T cell proliferation . ^^^ Interestingly , anti CD 86 monoclonal antibody ( mAb ) had little inhibitory effect on the MLR unless it was added in combination with anti CD 80 mAb . ^^^ These studies suggest that CD 80 on lung DCs can provide a costimulatory signal to allogeneic T cells in the absence of CD 86 signaling , but that CD 86 functions poorly except when CD 80 is also engaged . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are indispensable for T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometry investigation of phenotypic markers including CD1a , HLA DR , CD 80 ( BB1 / B7 . 1 ) and CD 86 ( B70 / B7 . 2 ) , and functional status ( stimulatory potential in allogeneic mixed leucocyte reactions ( MLR ) ) confirmed the generation of cells phenotypically identified as cultured DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blockade of the interactions between CD 28 and its ligands , CD 80 and CD 86 , prolongs the survival of allografts in some transplantation models . ^^^ However , we found that allogeneic hepatocytes were completely rejected within 7 days after intrasplenic transplantation , even when treated with monoclonal antibodies ( mAbs ) against CD 80 and CD 86 ( anti CD80 / 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential distribution of B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) costimulatory molecules on mucosal macrophage subsets in human inflammatory bowel disease ( IBD ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using flow cytometry and reverse transcription PCR analyses , the expression of molecules belonging to the tumor necrosis factor receptor ( TNFR ) and tumor necrosis factor ligand ( TNFL ) families , as well as the expression of CD 80 and CD 86 molecules , was analyzed in normal B cells and in different chronic lymphoproliferative disorders of B cell type , including B cell chronic lymphocytic leukemia ( CLL ) , mantle cell lymphoma , hairy cell leukemia ( HCL ) , and HCL variant . ^^^ TNFR 2 ( CD120b ) and CD 80 were distributed on neoplastic B cells from all groups , usually at an intermediate to high degree of intensity , whereas the CD 86 molecule was present at lower intensity than CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
G 7 DC not only expressed class 1 and class 2 MHC , CD1a , CD11c , CD 23 , CD 40 , CD 54 , CD 58 , CD 80 , CD 86 and CD 95 , like G 4 DC , but also CD 21 , which is the complement receptor type 2 , a ligand for CD 23 and a receptor for EBV and IFN alpha . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on APC provide a major costimulatory signal through interactions with CD 28 on T cells . ^^^ Absent from resting human T cells , CD 86 is up regulated early upon T cell activation , whereas CD 80 expression appears later . ^^^ Whereas T cell expression of CD 80 has been implicated in costimulation , the functional significance of CD 86 expression on T cells is unclear . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cultured cells from the HIV 1 infected subjects had similar morphology and phenotype of mature DC ( CD 80 = 41 + / 8 % , CD 86 = 77 + / 5 % , CD 40 = 87 + / 6 % , CD1a = 1 + / 1 % ) to DC cultured from seronegative subjects . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Transduced or untransduced dendritic cell progeny expressed comparable levels of HLA DR , CD 83 , CD1a , CD 80 , CD 86 , S 100 , and p 55 antigens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypic analysis of cultured DCs from BM augmented patients , unlike that of controls , exhibited a marked down regulation of B 7 1 ( CD 80 ) while retaining normal levels of expression of B 7 2 ( CD 86 ) cell surface molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BACKGROUND : Small , resting B cells ( rB cells ) express major histocompatibility complex ( MHC ) class 2 molecules but not the putative costimulatory molecules , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) ; they are classified as nonprofessional antigen presenting cells . rB cells have been shown to be capable of anergizing T cells in vitro and inducing the prolonged survival of skin grafts mismatched for a single minor histocompatibility ( miH ) antigen , H Y . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here we show that virtually all germinal center ( GC ) derived B cell lymphomas including follicular lymphoma ( FL ) and diffuse large cell lymphoma , but not mantle cell lymphoma or small lymphocytic lymphomas ( SLL / CLL ) , express B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on their cell surface in situ , although at extremely low levels . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both types of Mo DC expressed OX40L after stimulation through CD 40 ; ligation of OX40L on activated IL 4 Mo DC enhanced by 4 to 35 fold their cytokine production ( TNF alpha , IL 12 p 40 , IL 1 beta , and IL 6 ) and increased CD 80 , CD 86 , CD 54 , and CD 40 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cells from both sources were DEC 205+ , CD11c+ , MHC class II+ , CD 80 ( low ) ( i . e . , low level of CD 80 ) , CD 86 ( low ) and CD 40 ( low ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CTLA 4 ligands CD 80 and CD 86 were simultaneously expressed in most CTLA 4 negative lymphoma cases . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Best understood of these costimulatory interactions are those between CD 28 and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Under these culture conditions , by day 21 , a fraction of cells had lost CD 14 and acquired both CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 ( CD 152 ) , high avidity receptor for CD 80 and CD 86 , is a powerful regulator of T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD34+ and monocyte derived DCs were characterized by a similar proportion of CD1a+ cells expressing HLA class 2 antigens and CD 54 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Finally , we found that the virulent Listeria induced macrophages expressed a higher level of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules than did the avirulent Listeria induced macrophages and naive peritoneal macrophages . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The percentage of B cells expressing CD 80 , CD 86 , and the level of expression of CD 20 is higher in blood than in LN . ^^^ In contrast to humans , a relatively high proportion of T cells in blood also express the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Ligation of CD 40 on antigen presenting cells by its cognate ligand CD40L is thought to induce expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Because the B7 / CD28 costimulatory pathway has been shown to influence the differentiation of Th cell subsets , we investigated the contribution of the B 7 molecules CD 80 and CD 86 to the Th 2 cytokine profile and development of arthritis in BALB / c mice infected with Borrelia burgdorferi . ^^^ Moreover , combined CD80 / CD86 blockade by using anti CD 80 and anti CD 86 monoclonal antibodies or CTLA 4Ig enhanced IFN gamma production over that seen with CD 86 blockade alone , yet augmentation of this Th 1 associated cytokine did not enhance disease . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The second , costimulatory signal can be provided by cell surface molecules on antigen presenting cells such as B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , which interact with CD 28 on T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 80 , CD 86 , and ICAM 1 on lymphoma cells was up regulated by soluble CD 40 ligand . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In these in vivo studies , CD 28 costimulation did not show a significant benefit , possibly because of the high level expression of CD 80 and CD 86 on the surface of the lymphoma cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : With flow cytometric analysis we have investigated the expression of the costimulatory molecules B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and CD 18 and the intercellular adhesion molecule 1 ( ICAM 1 ) on peripheral blood monocytes and the expression of the activation markers HLA DR and IL 2R ( CD 25 ) on peripheral blood T lymphocytes from 31 CD patients , 17 ulcerative colitis ( UC ) patients , and 10 healthy controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we confirm the absence of immune costimulatory molecules , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , on leukaemia cells and demonstrated that these costimulatory molecules on the leukaemia cells can be upregulated by a combination of GM CSF and IL 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The Mox 2 gene maps to Chr 16 , telomeric to the clustered T cell costimulatory molecules Cd 80 and Cd 86 ( Reeves et al . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 25 ( IL 2 receptor alpha chain ) and CD 28 , characteristic of T cells , were detected on the surface of these cells ; this latter finding rises the possibility that enterocytes could be activated by IL 2 and / or via CD 28 through binding to its ligands CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Equivalent levels of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were found on the two DC subtypes and co stimulator assays did not reveal any functional differences between them . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Role of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) in the immune response to an intracellular pathogen . ^^^ The costimulatory ligands CD 80 and CD 86 play a crucial role in the initiation and maintenance of an immune response . ^^^ We demonstrate that whereas infection of human monocytes with viable tachyzoites of Toxoplasma gondii resulted in rapid induction of expression of CD 80 and up regulation of expression of CD 86 , incubation with killed organisms failed to alter the levels of expression of these costimulatory ligands . ^^^ Proliferation of resting T cells in response to parasite infected cells was dependent on both CD 80 and CD 86 . ^^^ More importantly , early production of IFN gamma in response to T . gondii by T cells from T . gondii seronegative individuals occurred only after stimulation with monocytes that exhibited increased expression of CD 80 and CD 86 ( monocytes infected with viable parasites ) and was almost completely ablated by the combination of anti CD 80 plus anti CD 86 mAb . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cryptococcus neoformans differently regulates B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression on human monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show that hepatocytes which do not express CD 80 and CD 86 co stimulatory molecules are able to induce activation and effective proliferation of specific naive CD8+ T cells in the absence of exogenously added cytokines , a property only shared by professional antigen presenting cells ( APC ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The individual roles of CD 80 and CD 86 in autoimmune diseases are complicated by multiple factors in vivo . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that expression of costimulatory molecules such as CD11a , CD 40 , CD 54 , CD 58 , CD 80 and CD 86 did not vary significantly between caM phi and aaM phi and was low for CD 58 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 12 reverses established antigen specific tolerance of contact sensitivity by affecting costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On culture with granulocyte macrophage CSF , this population differentiated into dendritic cells expressing CD 83 , CD 80 , HLA DR ( bright ) , CD 86 , and CD 54 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blockade of the ligands for CD 28 and CTLA 4 , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , revealed distinct and nonoverlapping function . ^^^ Blockade of CD 80 at initial immunization completely abrogated primary and secondary Ab responses , whereas blockade of CD 86 suppressed primary but not secondary responses . ^^^ Simultaneous blockade of CD 80 + CD 86 was less effective at suppressing Ab responses than either alone . ^^^ These findings suggest complex and distinct roles for CD 28 , CTLA 4 , CD 80 , and CD 86 in T cell costimulation following nucleic acid vaccination . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of CD 80 and CD 86 was up regulated in the memory B cells , compared to naive B cells and plasma cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypically , all the induced cell types expressed CD1a , CD 80 , CD 86 , HLA DR , and CD 68 in a magnitude similar to that of non treated monocyte derived DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These molecular changes include neo expression of the DC associated surface molecules cluster of differentiation ( CD ) 1a , CD1b , CD1c , human leukocyte antigen ( HLA ) DR , HLA DQ , CD 80 , CD 86 , CD 40 , CD 54 , and CD 5 , and downregulation of CD 15 and CD65s . ^^^ Additional stimulation with CD 40 ligand induces also expression of CD 83 and upregulates CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells expressed class 1 and class 2 MHC , CD1a , CD 2 , CD 4 , CD11c , CD 40 , CD 45 , CD 80 , CD 83 , and CD 86 and were potent stimulators of allogeneic T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that the accessory cell activity of monocytes during LPS induced T cell proliferation is characterized by the following features : LPS primed monocytes are competent stimulators of T cell proliferation ; interaction of LPS with monocytes during the priming step is dependent on CD 14 and is sensitive to ammonia ; monocyte / T cell interactions are not MHC restricted but are strongly dependent on interactions of CD 28 and / or CTLA 4 on T cells and their ligands CD 80 and / or CD 86 on monocytes . ^^^ CD 80 seems to be crucial for the activation of T cells by monocytes , since monocytes expressing CD 86 but not CD 80 after LPS stimulation were unable to stimulate T cells ; IL 12 , at least as a costimulatory factor , but not IL 15 , is important in LPS induced T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A crucial role of host CD 80 and CD 86 in rat cardiac xenograft rejection in mice . ^^^ METHODS : We investigated the differential roles for direct and indirect antigen presentation by preventing the CD 28 costimulatory pathway with monoclonal antibodies to rat or mouse CD 80 and CD 86 in a rat to mouse cardiac transplantation model . ^^^ RESULTS : Although the mouse anti rat monoclonal antibodies to CD 80 and CD 86 did not significantly prolong the survival of rat cardiac xenografts in mice , the rat anti mouse monoclonal antibodies to CD 80 and CD 86 did prolong the survival . ^^^ CONCLUSIONS : These findings suggest that the indirect antigen presentation , which is mediated by CD 80 and CD 86 pathway on host APC , plays a crucial role in concordant cardiac xenograft rejection . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 shares important sequence homology with CD 28 and binds to the same ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ We have developed new monoclonal antibodies against human CTLA 4 and have investigated the in situ expression of CTLA 4 in a wide variety of normal and pathological human tissues expressing CD 80 and CD 86 . ^^^ Interestingly , no or at most scarce expression of CTLA 4 was found in granulomatous lymph nodes , T cell mediated inflammatory diseases , or non Hodgkin ' s lymphomas , regardless of their expression of CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immunoliposomes containing monoclonal antibodies ( MAbs ) to the costimulatory molecules CD 28 and CTLA 4 and their counterreceptors B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were evaluated for the ability to increase the immune response to recombinant envelope protein rgp 120 of the MN strain of human immunodeficiency virus type 1 ( HIV 1 ) during vaccination . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Functional consequences of dysregulated B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression in B or T lymphocytes of transgenic mice . ^^^ We have generated transgenic mouse strains that constitutively express B 7 1 ( CD 80 ) at high levels on B cells or T cells or express B 7 2 ( CD 86 ) on T lymphocytes to examine the consequences of dysregulated B 7 expression on T cell responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This was not related to binding of CTLA4Ig or CD40L fusion proteins , implying similar levels of expression of their ligands , CD 80 and CD 86 or CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Overnight cultured DC from livers of FL treated animals expressed both higher levels of costimulatory molecules ( CD 80 and CD 86 ) and allostimulatory activity than those from controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Examination of the surface expression of MHC class 2 , adhesion molecules ( CD11a , CD11b , CD 48 , CD 54 , and CD 102 ) or costimulatory molecules ( CD 24 , CD 80 , and CD 86 ) showed no differences between male and female derived Mac 3+ APC populations . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Several in vitro and in vivo models have demonstrated a role for the costimulatory molecules , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , in the generation of distinct effector T cell responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The inhibitory effects of T : T presentation were not due to a lack of costimulation , since the T cells expressed levels of CD 80 and CD 86 higher than those detected on cultured dendritic cells and equipped them to function as efficient APCs for primary CD8+ T cell responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both types of DC expressed high levels of CD1a , MHC class 2 , CD 80 , CD 86 and CD 40 and were potent stimulators of LY proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Thus , the expression of IL 7 by DC follows the pattern described previously for CD 80 , CD 86 and CD 40 . ^^^ However , tissue culture of purified resting / immature DC , in contrast to CD 80 , CD 86 and CD 40 , failed to induce IL 7 transcripts . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After 24 hr of culture , they upregulated the expression of MHC 2 , CD 40 , CD 80 , and CD 86 to levels just as high as those on DCs from organ transplant donors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The absence of autologous tumour specific cytolytic T cell ( CTL ) activity may be due to the poor immunogenicity of colorectal tumour cells , which we found expressed only low levels of MHC 1 antigens and CD 54 and failed to express MHC 2 antigens or the co stimulatory molecules CD 80 , CD 86 or CD 106 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recent studies have suggested that the accessory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) differ in their capacity to generate Th 1 vs Th 2 responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
PURPOSE : To examine normal and inflamed conjunctiva from patients with ocular cicatricial pemphigoid ( OCP ) for the presence of costimulatory molecule CD 28 and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Growth and differentiation of DC was followed by an increase in expression of MHC 2 and co stimulating molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
One clone showed sequence homology to a gene encoding a molecule on rat dendritic cells ( MRC OX 2 ) , with homology to genes encoding the costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules ( CD 80 and CD 86 ) on Reed Sternberg cells are associated with the proliferation of background T cells in Hodgkin ' s disease . ^^^ To elucidate the relationship between Reed Sternberg ( R S ) cells and background T cells , the expression of CD 80 and CD 86 of R S cells in Hodgkin ' s disease ( HD ) , and the ligand CD 28 expression and the MIB 1 index of background T cells were immunohistochemically investigated . ^^^ CD 80 and CD 86 were found to be expressed on R S cells in almost all cases of HD . ^^^ These results suggest that the interaction between CD 80 and CD 86 on R S cells , and CD 28 on background T cells may induce T cell proliferation and be associated with tumor mass of HD . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules implicated in differential Th1 / Th2 commitment CD 80 , CD 86 , and IL 12 are not inducible by SR ligation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of B 7 family costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on tumor cells enhances host immunity . ^^^ A critical test for the hypothesis is whether selective engagement of CTLA 4 receptors by their natural ligands CD 80 and CD 86 enhances or reduces antitumor immunity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In immunohistochemical analysis , CD 80 and / or CD 86 were detected on dendritic shaped cells not only in the epidermis but also in the dermis in the inflammatory lesions of atopic dermatitis ( n = 12 ) . ^^^ CD 80 was expressed in only five cases ( 42 % ) , while CD 86 was expressed in all cases ( 100 % ) . ^^^ In non lesional skin of atopic dermatitis ( n = 4 ) , CD 86 but not CD 80 was detected in one case . ^^^ The CD 80 or CD 86 positive cells were confirmed as Langerhans cells by double immunostaining using anti CD1a monoclonal antibody . ^^^ Neither CD 86 nor CD 80 was detected on keratinocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A stimulating anti CD 28 mAb , or CD 80 or CD 86 on stably transfected P 815 cells , provided the co stimulatory signal . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This observation emerged from studies aimed at using retrovirus vectors to genetically modify tumor cells to express the costimulatory proteins CD 80 and CD 86 . ^^^ Retrovirus mediated expression of CD 80 and CD 86 in T lymphoblastoid CEM cells resulted in an unexpected intracellular focal concentration of both proteins in the genetically modified cells . ^^^ By extending these studies to an analysis of CD 80 and CD 86 expression in PBMC , we observed that endogenous CD 86 expression in peripheral blood monocytes also involved a similar intracellular focal concentration of the protein . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
When compared with non TNFalpha supplemented cultures , these DC also displayed an increased surface expression of CD 83 as well as the costimulatory molecules , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Activation markers such as CD 10 , CD 38 , and CD 95 and T cell costimulatory molecules CD 80 and CD 86 , which were expressed by neoplastic follicles , were either downregulated or absent in the interfollicular component in most of the cases . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendritic cells that developed from myeloid precursors accumulated after 2 weeks in an intermediate CD1a , CD 80 , CD 83 , CD 86 stage . ^^^ This maturation involved increased expression of CD 80 , CD 83 , CD 86 , CMRF 44 , HLA A , B , C , and DR as well as downregulation of CD1a and CD11b . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Co stimulatory molecules B 7 1 ( CD 80 ) / B7 2 ( CD 86 ) and their counter receptors CD28 / CTLA 4 play pivotal roles in T cell activation and immune regulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Biased dependency of CD 80 versus CD 86 in the induction of transcription factors regulating the human IL 2 promoter . ^^^ One such co stimulatory signal is delivered when CD 28 on T cells binds to CD 80 or CD 86 on antigen presenting cells ( APC ) . ^^^ In the present study , we analyzed the ability of CD 80 and CD 86 to co stimulate human T cells activated by superantigen . ^^^ Using the Raji B cell lymphoma , which express similar levels of CD 80 and CD 86 , it was found that T cell proliferation was mainly co stimulated by CD 80 . ^^^ To further characterize the consequences of this biased co stimulatory dependency , we employed a well defined system of transfected CHO cells expressing human MHC class 2 together with CD 80 , CD 86 or CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The consequence of autoantigen presentation by thyroid cells is dependent on the magnitude of expression of both HLA class 2 antigens ( mainly HLA DR ) and costimulatory molecules , such as B 7 ( CD 80 and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This was significantly correlated with the expression of CD 28 ligand , i . e . , CD 86 ( but not CD 80 ) , and with an increase in the proliferative activity ( labeling index ) of myeloma cells in bone marrow . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on cerebrospinal fluid cells in multiple sclerosis . ^^^ The costimulatory CD 80 and CD 86 molecules were measured by flow cytometry on cerebrospinal fluid ( CSF ) and blood lymphocytes from patients with possible first attacks of multiple sclerosis ( MS , n = 25 ) , clinically definite MS ( n = 16 ) , and noninflammatory neurological disease control subjects ( n = 30 ) . ^^^ In patients with demyelinating diseases more CSF B cells expressed CD 80 than in control subjects whereas the expression of CD 86 by T cells in CSF was low in patients with demyelinating disease and highly variable in the control subjects . ^^^ In patients with possible first attacks of MS the expression pattern of CD 80 and CD 86 differed significantly between patients with or without intrathecal synthesis of IgG . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Despite the importance of the costimulatory proteins B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and their counterreceptors CD 28 and CTLA 4 ( CD 154 ) in the regulation of T cell proliferation in the adult immunological system , the initial appearance of these proteins during embryonic development has not been investigated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To determine whether B 7 signals are associated with atopic responses in man , we assayed CD 80 and CD 86 expression on B cells and monocytes from atopic patients and controls . ^^^ Peripheral blood mononuclear cells from 10 patients with perennial allergic rhinitis and from 10 normal subjects were cultured in the presence or absence of house dust mite antigen , and B cells and monocytes were assayed for expression of CD 80 and CD 86 by flow cytometry . ^^^ CD 86 on B cells was significantly and selectively upregulated in all atopic subjects , but not in normal subjects , whereas CD 80 expression was not altered in B cells from the atopic subjects or controls . ^^^ In contrast , both CD 80 and CD 86 were upregulated in monocytes from the atopic subjects as well as the controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells are directly transfected in vivo , present Ag , and display the surface proteins CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using transfected variants of the human colorectal cancer cell line SW 480 we tested various costimulatory molecules ( CD 80 , CD 86 , CD 54 ) and a class 2 major histocompatibility complex ( MHC ) allele ( HLA DR 3 ) alone or in combination on their ability to support primary T lymphocyte activation in vitro . ^^^ Expression of CD 80 or CD 86 similarly as the combination of both was not sufficient to induce proliferation of human allogeneic T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CpG ODN caused up regulation of MHC class 2 , CD 40 and CD 86 , but not CD 80 on immature and mature DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric triple fluorescence analysis was applied to study CD 14 , CD 16 , CD 33 , CD 44 , CD11b , CD11c , CD 64 , HLA DR , CD 80 , CD 86 and CD3 / CD19 expression . ^^^ Furthermore , expression of CD 80 ( 9 . 2 + / 4 . 2 % ) and CD 86 ( 15 . 1 + / 7 . 3 % ) was low , suggesting a low ability of normal intestinal macrophages to activate T cells and T cell mediated immune responses . ^^^ These cells exhibit a single phenotype in normal mucosa ( CD33++ , CD44++ , CD 14 , CD 16 , CD11b , CD11c , HLA DRlow , CD 80 , CD 86 ) lacking lipopolysaccharide ( LPS ) receptor and costimulatory molecules . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 40 ligand ( CD40L ) and B 7 2 ( CD 86 ) were also expressed , but to a lower extent , while B 7 1 ( CD 80 ) expression was limited . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : DCs isolated from livers of FL treated donor mice exhibited increased cell surface expression of CD 40 , CD 80 , CD 86 , and IAb , and augmented T cell allostimulatory activity compared with controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 16 cells and their transfected sublines were CD40+ CD44+ but expressed no ( or low levels of the ) costimulator molecules CD 154 ( CD40L ) , CD 48 , CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Circulating CD34+ cells were purified to > or =98 % by high gradient magnetic separation and then analyzed for the coexpression of HLA DR , the common beta chain of the leukointegrin family CD 18 and costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ Three color staining cytofluorimetry indicated that expression of CD 80 and CD 86 were upregulated in 6 . 9 + / 4 . 9 and 10 . 7 + / 2 . 6 % CD34+ blood cells respectively , after 24 30 h of culture with autologous or allogeneic mononuclear cells , or with CD4+ , or CD8+ T cells , but not with medium alone . ^^^ Blocking experiments demonstrated that preincubation of stimulator and responder cells with anti CD 80 plus anti CD 86 monoclonal antibodies induced a 84 + / 8 % inhibition of CD34+ cell allostimulating activity after 6 days in primary MLC . ^^^ These results suggest that G CSF mobilized CD34+ hematopoietic progenitors with alloantigen presenting function express CD 18 and may upregulate CD 80 and CD 86 upon interaction with T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In particular , the relative competence of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) to provide effective co stimulus is not well defined , and the identification of the T cell co stimulatory receptor that mediates B 7 co stimulation in tumor rejection has not been addressed . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
T cell alterations in cardiac allograft recipients after B 7 ( CD 80 and CD 86 ) blockade . ^^^ CD 28 binds both the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) ligands on antigen presenting cells . ^^^ We tested the hypothesis that in vivo blockade of the CD 28 pathway via the anti CD 80 and anti CD 86 monoclonal antibodies ( mAbs ) would prolong allograft survival . ^^^ METHODS : Neonatal C57BL / 6J ( H2b ) hearts were transplanted to CBA / J ( H2k ) recipients in a heterotopic nonvascularized model , with anti CD 80 and / or anti CD 86 mAbs being administered intravenously at the time of allografting ( day 0 ) and on the following day ( day 1 ) . ^^^ RESULTS : Anti CD 80 mAb ( 29 . 8+ / 1 . 5 days ) and anti CD 86 mAb ( 30 . 8+ / 0 . 5 days ) alone significantly prolonged allograft survival compared with the isotype control ( 10 . 7+ / 0 . 4 days , P < 0 . 01 , Wilcoxon rank sum ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This is accompanied by the upregulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , CD 25 , ICAM 1 and the DC specific marker CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that CD 40 ligation enhanced ICAM 1 expression and did not upregulate HLA DR , CD 80 or CD 86 expression on IFN gamma treated keratinocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After 14 days of culture in GM CSF , TNF alpha , and IL 4 , CD34+ progenitors gave rise to a population of large , immature DC expressing CD1a and CD11b but lacking CD 14 , CD 80 , CD 83 , CD 86 , and CMRF 44 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 86 ( B 7 2 ) , but not CD 80 ( B 7 1 ) , expression in the epidermis of transgenic mice enhances the immunogenicity of primary cutaneous Candida albicans infections . ^^^ Transgenic ( Tg ) mice whose epidermal keratinocytes constitutively overexpress either B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) exhibited exaggerated cutaneous delayed type hypersensitivity ( DTH ) to haptens compared to non Tg mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We studied the surface expression of B 7 1 and B 7 2 in 24 cases of gastric carcinoma from the primary locus , 20 cases of metastatic carcinoma from malignant ascites , 20 cases of benign gastric mucosa and 7 gastric carcinoma cell lines by two color flow cytometry with mAb CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The role of costimulation in activation for proliferation and cytokine mRNA production of peripheral blood T cells was studied by blocking CD 28 , CTLA 4 , and their ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ RESULTS : The proliferation and the production of type 1 and 2 cytokine mRNA by T cells in response to Der p as well as the control antigens TT and CA was inhibited by simultaneously masking CD 80 and CD 86 using CTLA 4 Ig , a soluble form of CTLA 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Activation dependent transcription and expression of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were detected by reverse transcription polymerase chain reaction and flow cytometry in the absence or presence of glucocorticoids . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Candidate genes include those coding for CD 80 and CD 86 , molecules involved in antigen specific T cell recognition . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Autoimmunity without diabetes in transgenic mice expressing beta cell specific CD 86 , but not CD 80 : parameters that trigger progression to diabetes . ^^^ To define more clearly the roles of CD 80 ( RIP CD 80 ) and CD 86 ( RIP CD 86 ) in the activation of autoreactive T cells in vivo , we generated transgenic mice expressing either or both costimulatory molecules on the beta cells of the pancreas . ^^^ While RIP CD 80 mice do not show any sign of autoimmunity , at the age of 7 mo RIP CD 86 transgenic mice develop a lymphoid infiltrate with both IFN gamma and IL 4 positive cells in the vicinity of the islets ; these mice , however , never progress to diabetes . ^^^ This fundamental difference in the ability of CD 80 and CD 86 to activate self reactive T cells in vivo is , however , obliterated when the level of TCR signaling is increased by either TNF alpha or transgenic MHC class 2 expression . ^^^ These results support the suggestion that CD 80 and CD 86 mainly differ at the level of the intensity of the signals they deliver . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
While the DC phenotype , HLA DR+CD14 , was expressed by > or =90 % of the cells generated from relatives and controls , the proportion of cells that expressed CD1a and the costimulator molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) was significantly lower in IDDM relatives . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
When IL 4 consumption was blocked , it became evident that CD 80 and CD 86 both provide effective costimulatory signals for high IL 4 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we analyzed the effect of UVB irradiation on the Langerhans cells expression of several accessory molecules , namely CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In an attempt to prevent rejection , we used cyclophosphamide ( CP ) , a powerful anti B cell agent , or CTLA4Ig , an inhibitor of T cell co stimulation [ via B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) ] , either given in combination with anti CD 4 ( GK1 . 5 ) or anti CD 3 ( KT 3 ) MAb to the recipient mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 needs to be cross linked to initiate signals , yet both of its ligands , CD 80 and CD 86 , are expressed as monomers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Studies using antigen presenting cells lacking expression of both B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) show distinct requirements for B 7 molecules during priming versus restimulation of Th 2 but not Th 1 cytokine production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The involvement of CD 80 and CD 86 costimulatory molecules in the induction of eosinophilia in mice infected with Nippostrongylus brasiliensis . ^^^ The costimulatory signal provided by the interaction between CD 28 and its ligands , CD 80 and CD 86 , is critical for T cell activation . ^^^ The requirement of CD 80 and CD 86 in T cell activation for eosinophilia and IgE production was examined in mice infected with the nematode parasite Nippostrongylus brasiliensis . ^^^ Combined treatment with anti CD 80 and anti CD 86 suppressed eosinophilia in the blood and the small intestine and suppressed IgE production . ^^^ However , administration of either anti CD 80 or anti CD 86 alone had little effect on eosinophilia and on the elevation of IgE levels . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules in Wegener ' s granulomatosis ( WG ) : lack of expression of CD 28 and preferential up regulation of its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on T cells . ^^^ Since the expression of costimulatory molecules has a significant impact on the cytokine profile and proliferation response of T cells , the goal of this study was to characterize the expression of costimulatory molecules ( CD 28 , CTLA 4 ( CD 152 ) , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) ) on T cells , monocytes and B cells in WG , and to correlate the findings with clinical parameters such as disease activity , extent and therapy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Because it is suggested that costimulatory molecules , such as CD 80 and CD 86 , differentially stimulate Th 1 and Th 2 cells we wished to investigate the role of these costimulatory molecules in the activation of immune suppression . ^^^ We injected UV irradiated mice with monoclonal antibodies to CD 80 and CD 86 and asked what effect , if any , this would have on UV induced immune suppression . ^^^ Anti CD 86 , but not anti CD 80 or control rat IgG , blocked UV induced immune suppression . ^^^ IL 10 was detectable in the serum of UV irradiated mice as compared with normal controls , and injecting UV irradiated mice with anti CD 86 , but not anti CD 80 or control rat IgG , blocked the secretion of IL 10 into the serum . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Ligation of CD 28 molecules expressed on the surface of human leukaemic natural killer like YT cells triggers intracellular signals leading to cytolysis of target cells expressing CD 80 or CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To study the prerequisites of functional interactions between malignant B cells and intermingled T cells in B cell non Hodgkin ' s lymphomas ( B NHL ) , we examined the expression of CD 40 , CD 80 and CD 86 and their ligands CD 40 ligand ( CD40L , CD 154 ) , CD 28 and CTLA 4 ( CD 152 ) using immunohistochemistry and confocal laser scanning microscopy . ^^^ Almost all mucosa associated lymphoid tissue ( MALT ) NHL were positive for CD 40 and CD 80 and in nine out of 14 cases were positive for CD 86 . ^^^ The majority of follicle centre cell lymphomas ( FCCL ) expressed CD 40 , but were heterogeneous in their expression of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules , CD 80 and CD 86 ] . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Unlike ligation of CD 3 , stimulation with anti CD 28 mAb or CHO cells expressing the CD 28 ligands CD 80 or CD 86 did not lead to tyrosine phosphorylation of HS 1 in Jurkat T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To enhance a DNA vaccine ' s ability to induce CTL response in vivo , we co administered CD 80 and CD 86 expression cassettes along with HIV 1 immunogens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Such Ts cells express the CD8+CD28 phenotype and show the following characteristics : ( a ) antigen specificity and restriction by self MHC Class 1 molecules ; ( b ) limited TCR 5 beta gene usage ; ( c ) ability to inhibit antigen specific , MHC Class 2 restricted , Th proliferative responses ; and ( d ) capacity to downregulate and / or inhibit the upregulation by Th of CD 40 , CD 80 , and CD 86 molecules on APCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We also observed a more rapid loss of surface class 2 antigen expression in the ethanol , acetaldehyde , and acetate treated 43HIV cells , but no change in surface expression of CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The enhanced protection conferred by Flu ISCOMs in aged mice correlates with the up regulation of co stimulatory molecule , CD 86 ( B7 . 2 ) and to a lesser extent , CD 80 ( B7 . 1 ) expression on antigen presenting cells . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Parasite internalization was associated with activation manifested by upregulation of major histocompatibility complex ( MHC ) class 1 and 2 surface antigens , increased expression of costimulatory molecules ( CD 40 , CD 54 , CD 80 , and CD 86 ) , and interleukin ( IL ) 12 p 40 release within 18 h . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Regarding LC maturation in epidermal cell cultures , IL 6 and IL 10 were inhibitory for the expression of CD 86 and CD 80 in a dose dependent fashion . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The effect of immunotoxins directed against CD 80 and CD 86 on primary T cell alloresponses . ^^^ Activation of primary resting T cells requires costimulation which can be delivered by the B 7 molecules ( CD 80 and CD 86 ) expressed on activated antigen presenting cells ( APC ) . ^^^ In the present study , we examined in vitro effects of immunotoxins ( ITs ) composed of gelonin conjugated to mAbs against CD 80 or CD 86 ( alphaCD 80 IT and alphaCD 86 IT ) . ^^^ The specificity of both ITs was demonstrated using CD 80 and CD 86 transfected cell lines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Of note , CD 86 provides the initial signal to induce naive T cells to become IL 4 producers , whereas CD 80 is a more neutral differentiation signal . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blood derived DC up regulated MHC class 2 , CD 54 , CD 80 and CD 86 after exposure to WV vaccine , indicating their functional maturation , but were only moderately affected by subunit ( SU ) vaccines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 represent ligands for this receptor , and although they are present on professional Ag presenting cells , these molecules are absent from most tumors . ^^^ Therefore , a key question concerns the molecular basis for the costimulation of T cells by those tumor cells not expressing the CD 28 ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Further , 9 cryosections from MF lesions and 9 from SS lesions were stained for LFA , ICAM 1 , CD 40 , CD 40 ligand , CD 28 , CD 80 , CTLA 4 , CD 86 , FAS , FAS ligand , CLA and CD15s . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Harvested DCs represented yields of 4 . 1+ / 1 . 4 and 5 . 8+ / 5 . 4 % of the initial cells plated from the CD34+ cell depleted mobilized PBMCs of normal donors and cancer patients , respectively , and displayed a high level expression of CD 80 , CD 86 , HLA DR , and CD11c but not CD 14 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of MHC class 2 , CD 80 and CD 86 on EC was not affected by IL 12 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Human muscle cells express a functional costimulatory molecule distinct from B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) in vitro and in inflammatory lesions . ^^^ The B 7 family of costimulatory molecules likely includes members distinct from B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We therefore examined CD 80 and CD 86 dependence of allergen induced T cell proliferation and cytokine production in peripheral blood and bronchoalveolar lavage from atopic asthmatic subjects and controls . ^^^ Both allergen induced proliferation and IL 5 production from PBMC were inhibited by CTLA 4 Ig fusion protein and anti CD 86 , but not anti CD 80 mAbs . ^^^ When allergen specific CD4+ T cell lines from peripheral blood were examined , proliferation and cytokine production were found to be independent of CD 80 or CD 86 costimulation . ^^^ However , when cells obtained directly from the airways were examined , allergen induced proliferation of bronchoalveolar lavage T cells from atopic asthmatic subjects was inhibited by anti CD 86 but not anti CD 80 . ^^^ This was also inhibited by anti CD 86 but not anti CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show that the CD 40 induced production of IL 6 , IL 8 and TNF alpha by monocytes , and IL 12 by dendritic cells , and expression of the activation markers CD 83 , the costimulatory molecules CD 86 and CD 80 , and HLA DR antigens were all similar in patient and control cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 10 decreased the constitutive surface expression of MHC class 2 as well as of the accessory molecules CD 80 and CD 86 on LSEC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A flow cytometric analysis showed that a slight expression of CD 80 ( B 7 1 ) on monocytes was induced by NRC treatment , whereas the constitutive expressions of CD 86 ( B 7 2 ) and CD 54 ( ICAM 1 ) were unchanged . ^^^ The activation of monocytes with interferon gamma ( IFN gamma ) induced the expressions of CD 80 , CD 86 , and CD 54 under all conditions tested , but NRC treatment tended to decrease the IFN gamma induced expression of CD 54 on monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Studies of cell surface markers showed that the 2 day cultured cells had up regulated class 2 major histocompatibility complex and CD 80 , but not CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both CD 80 and CD 86 co stimulatory molecules regulate allergic pulmonary inflammation . ^^^ We examined the roles of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) in a model of allergic pulmonary inflammation and airway hyper responsiveness ( AHR ) by selectively inhibiting either CD 80 or CD 86 . ^^^ Inhibition of co stimulation by either CD 80 or CD 86 affected multiple parameters of the allergic response . ^^^ Specifically , blockade of either CD 80 or CD 86 in ovalbumin sensitized and challenged mice resulted in reduced expression of IL 2Ralpha ( CD 25 ) on CD4+ T lymphocytes , decreased airway eosinophilia , lower serum IgE production and diminished AHR . ^^^ Importantly , blockade of CD 80 and CD 86 inhibited production of IL 4 and IL 2 , and enhanced IFN gamma production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Comparative analysis of CD 80 and CD 86 on human Langerhans cells : expression and function . ^^^ Although both CD 80 ( B 7 1 ) and CD 86 ( B 7 2 / B70 ) have been recently identified in cultured human Langerhans cells ( LC ) , little is known of the role and regulatory properties of CD 80 and CD 86 on human LC . ^^^ We present here the results of a study comparing the expression and function of CD 80 and CD 86 in human LC using the T helper type 1 cytokines IL 2 and interferon gamma ( IFN ) gamma , and the T helper type 2 cytokines IL 10 , IL 4 and granulocyte / macrophage colony stimulating factor ( GM CSF ) . ^^^ Freshly isolated human LC expressed little CD 80 and CD 86 in vitro , but the expression of both molecules was rapidly induced during a 72 h incubation with cytokines and the expression of CD 86 occurred much earlier and more strongly than that of CD 80 . ^^^ The expression of both CD 80 and CD 86 was upregulated by GM CSF and downregulated by IL 10 , and the expression of CD 86 , but not that of CD 80 , was upregulated by both IL 4 and IFN gamma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This can be provided by the accessory T cell molecule CD 28 upon binding to its respective ligands B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
VAL significantly upregulated the expression of the co stimulatory molecule B7 . 1 ( CD 80 ) on monocytes / macrophages , but not B7 . 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules CD 80 and CD 86 in the rat ; tissue distribution and expression by antigen presenting cells . ^^^ Only recently rat CD 80 and CD 86 have been cloned and monoclonal antibodies have been generated . ^^^ The target tissue of cyclosporin A induced autoimmunity , i . e . the skin and tongue , were also examined for expression of CD 80 and CD 86 . ^^^ Whereas CD 80 was hardly detected in the lymphoid tissues , CD 86 was clearly expressed by non lymphoid cells in the thymus , as well as in the secondary lymphoid organs . ^^^ On development of cyclosporin A induced autoimmunity , non professional APC , i . e . epithelial cells , started to express MHC class 2 , but not the costimulatory ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 4 + GM CSF , a combination of cytokines known to induce differentiation of dendritic cells , increased the expression of CD 40 , CD 80 and CD 86 , and decreased the expression of CD 14 on SF macrophages . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They also express B 7 costimulatory molecules ( CD 80 , CD 86 ) , CD 83 , and CD1a molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , interleukin 12 and transforming growth factor beta mRNA expression in CSF and peripheral blood mononuclear cells from multiple sclerosis patients . ^^^ Costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are differently involved in T cell stimulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells and cytokine levels ( IL 1alpha , IL 1beta , IL 2 , IL 6 , IL 8 , IL 10 , IL 13 , TNF alpha and IFN gamma ) in the afferent lymph were analyzed by cytofluorometry and ELISA . ^^^ However , no changes in the expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells were detectable . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The addition of anti CD 54 , CD 40 , CD 80 and CD 86 monoclonal antibodies to the cultures showed that the PBMC required only CD 54 and CD 86 for stimulation with C . albicans , but required CD 54 , CD 80 and CD 86 for stimulation with D . farinae . ^^^ Since many studies have suggested that T helper type 1 and T helper type 2 immune responses are different in their dependency on CD 80 or CD 86 costimulation , our present results suggest that the allergen specific T cells of AD patients are not completely shifted to a T helper type 2 subset . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
While it was found that systemic administration of mouse CTLA 4 Ig could also inhibit the progression of effector immune responses when administered shortly before or during clinical disease , these were significantly more active when delivered directly into the CNS , which probably involved an action on both CD 28 ligands , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) expression was present on the plasma cells of only 1 of 27 samples but CD 86 ( B 7 2 ) expression within the normal range was present on the plasma cells of 14 of 27 samples . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The allergen induced secretion of IL 5 and IL 13 was inhibited by the fusion protein CTLA 4Ig , reflecting a requirement for CD 80 ( B 7 1 ) and / or CD 86 ( B 7 2 ) costimulation for the expression of the Th 2 cytokines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BACKGROUND : Administration of donor bone marrow ( BM ) derived dendritic cell ( DC ) progenitors ( DCp ) that are major histocompatibility complex ( MHC ) class II+ but costimulatory molecule ( CD 40 , CD 80 , CD 86 ) deficient can prolong mouse heart allograft survival This is associated with microchimerism and inhibition of antidonor cytotoxic T lymphocyte ( CTL ) activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Since it contains a high percentage of CD 28 and CD57+ cells , is increased in size and granularity , and is transiently expressed following in vitro stimulation of naive CD8+ T cells , we speculate that this subset mainly represents recently activated effector T cells that are able to interact with CD 80 and CD 86 ( B 7 1 and B 7 2 respectively ) negative tissue cells . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast to CD 86 expression which remained as intensive in HIV+ as in HIV lymph nodes , CD 80 staining was strongly decreased in GC of HIV+ lymph nodes but not in their extrafollicular zones . ^^^ In contrast to HIV situations where CD 80 and CD 86 was similarly expressed on B cells , the lower level of CD 80 expression in HIV+ GC may favor Th 2 T cell responses through CD 86 CD28 interactions . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 17 promoted the maturation of DC progenitors , as evidenced by increased cell surface expression of CD11c , costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , and MHC class 2 Ag , and allostimulatory capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In order to elucidate the possibility of costimulatory molecules mediated immuno or immuno gene therapy for human hematological malignancies , we analyzed 30 hematopoietic cell lines and cells obtained from 48 patients with hematological malignancies for the expression of costimulatory molecules such as CD 80 and CD 86 . ^^^ The expression of CD 80 or CD 86 was frequent on cell lines derived from the patients with CML BC or Burkitt ' s lymphoma , while it was rare on cell lines from T ALL . ^^^ In 30 AML samples , CD 86 was positive in 15 cases ( 50 % ) , which was sharply in contrast with the finding that CD 80 was not detected in any AML samples . ^^^ We also treated seven human hematopoietic cell lines with IFN gamma , IL 12 or IL 15 and observed whether these cytokines could induce or enhance the expression of CD 40 , CD 54 , CD 58 and HLA DR as well as CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show here that 1 ) F 2 g3p was recognized with anti CTLA 4 mAb but not with anti CD 28 mAb , and 2 ) F 2 g3p bound to CD 80 but not to CD 86 . ^^^ F 2 g3p inhibited the binding of CTLA 4 to CD 80 but not to CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 are not equivalent in their ability to induce the tyrosine phosphorylation of CD 28 . ^^^ Ligation of either CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) , two principal ligands for CD 28 , is thought to skew the immune response toward Th 1 or Th 2 differentiation . ^^^ We have examined early signal transduction pathways recruited following T cell stimulation with either CD 80 or CD 86 . ^^^ Purified human peripheral T cells or Jurkat T cells were stimulated with Chinese hamster ovary ( CHO ) cells expressing either human CD 80 ( CHO CD 80 ) or human CD 86 ( CHO CD 86 ) or with anti CD 28 monoclonal antibody ( mAb ) . ^^^ In the presence of phorbol 12 myristate 13 acetate , both CHO CD 80 and CHO CD 86 , like anti CD 28 mAb , were capable of stimulating cytokine production from both human peripheral T cells and Jurkat T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Co stimulatory factors B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) and their ligands , including CD 28 , are important for the efficient presentation and persistence of an antigen specific immune reaction . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present study we examined the expression of CD 40 , CD 40 ligand ( CD40L ) , CD 80 , CD 86 , CD 28 and cytolytic T lymphocyte associated antigen 4 ( CTLA 4 ) on lymphocytes immunohistochemically . ^^^ In vitro kinetics of the expression of CD40L and CTLA 4 by peripheral blood T cells and that of CD 80 and CD 86 by peripheral blood B cells were also investigated by flow cytometry . ^^^ Positive percentage expression of CD40L , CD 28 and CTLA 4 , and CD 40 , CD 80 and CD 86 was calculated for the number of CD3+ and CD19+ cells , respectively . ^^^ Flow cytometric analysis demonstrated that the expression of CD40L and CTLA 4 on T cells , and CD 80 and CD 86 on B cells of peripheral blood was up regulated upon activation . ^^^ While most T cells and B cells expressed CD 28 , and CD 80 and CD 86 , respectively , in gingival tissues , the expression of CD40L and CTLA 4 was lower but highly variable between specimens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The accessory effect was inhibited by pretreatment with anti CD 72 , anti B 7 ( CD 80 and CD 86 ) , and anti IL 15 monoclonal antibodies ( MoAb ) . ^^^ A 24 hour stimulation of IL 15Ralpha+ / gammac+ macrophages with IL 15 upregulated the expression of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , endotoxin treatment down regulated surface expression of constitutively expressed MHC class 2 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The role of CD 80 , CD 86 , and CTLA 4 in alloimmune responses and the induction of long term allograft survival . ^^^ Blocking the interaction of the CD 28 costimulatory receptor with its ligands , CD 80 and CD 86 , inhibits in vivo immune responses , such as allograft rejection , and in some instances induces tolerance . ^^^ Previously , we found that CTLA4Ig , which blocks the CD28 / CTLA 4 ( CD 152 ) ligands CD 80 and CD 86 , can be used to induce transplantation tolerance to vascularized allografts . ^^^ Here , we show that blockade of CD 152 using an anti CD 152 mAb at the time of transplantation prevents the induction of long term allograft survival by agents that target CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The yield as well as the pattern and intensity of Ag ( HLA DR , CD 40 , CD 54 , CD 80 , and CD 86 ) expression were equivalent on DCs from BM or PB of MM patients versus normal donors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 costimulatory molecules on circulating T cells of HIV infected individuals . ^^^ The expression of CD 80 and CD 86 costimulatory molecules , typical for antigen presenting cells ( APC ) , was measured on circulating T cells of 20 HIV infected individuals and of 11 HIV negative healthy controls . ^^^ The CD 80 and CD 86 molecules were present on both circulating T subsets of HIV infected individuals ( mean of CD 80 expression within CD4+ T cells [ CD80 / CD4 ] : 5 . 0 % ; and CD86 / CD4 : 2 . 6 % ; CD80 / CD8 4 . 1 % and CD86 / CD8 : 2 . 7 % ) and were associated with HLA DR expression . ^^^ Some CD 80 and CD 86 expression was also found in normal controls , and only the expression of CD 86 was significantly ( P < 0 . 05 ) increased on CD 4 + and CD 8 + T cells of HIV infected individuals . ^^^ This suggests that during HIV infection T cells themselves develop an antigen presenting phenotype by upregulating expression of HLA DR , CD 86 and CD 80 molecules . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both cell lines express the same light ( kappa ) and heavy chains ( mu , delta ) as the fresh parental B CLL cells at the same high intensity , share the expression of mature B cell markers ( CD 19 , CD 20 , CD 21 , CD 22 ) , differ in the expression of CD 23 and FMC 7 , are CD11a+ , CD18+ , CD44+ , CD49d+ , CD54+ and express at high levels both CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential expression and modulation of costimulatory molecules CD 80 and CD 86 on monocytes from patients with systemic lupus erythematosus . ^^^ This study was undertaken to further investigate the expression and cytokine regulation of both CD 80 and CD 86 ( B 7 2 ) on monocytes from patients with SLE . ^^^ Freshly isolated and in vitro cytokine stimulated peripheral blood mononuclear cells from 13 patients with SLE and 10 healthy subjects were analysed , cytometrically with dual fluorescence staining , to detect expression of CD 80 and CD 86 in the CD14+ monocyte population . ^^^ The results showed that , as in normal individuals , an overwhelming majority ( 95 . 62+ / 3 . 54 % ) of monocytes from patients with SLE expressed the CD 86 molecule , but only a few monocytes ( 5 . 54+ / 4 . 36 % ) had detectable CD 80 expression . ^^^ The effects of interleukin 10 ( IL 10 ) on the expression of CD 80 and CD 86 on monocytes from patients with SLE and normal controls were similar . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Increased expression of CD 80 and CD 86 in in vitro infected CD3+ cells producing cytoplasmic HIV type 1 p 24 . ^^^ A striking proportion of p24+ cells expressed CD 80 or CD 86 , antigens not usually expressed by CD3+ lymphocytes . ^^^ CD 28 expression was generally comparable between CD3+ cells that did and did not express CD 80 or CD 86 . ^^^ Using this approach , we found an association between productive infection and increased expression of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Rather , CD 8 ( + ) T cell apoptosis was caused by antigen presenting cells because removal of monocytes or addition of antibodies to CD 80 and CD 86 reduced apoptosis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 80 and CD 86 on peripheral blood T lymphocytes in patients with systemic lupus erythematosus . ^^^ CD 80 and CD 86 were detected in high amounts on circulating T cells in the peripheral blood of some patients with systemic lupus erythematosus ( SLE ) , using flow cytometry and monoclonal antibodies . ^^^ Patients with other connective tissue diseases did not have a high percentage of T cells expressing CD 80 or CD 86 in their peripheral blood . ^^^ CD 80 was expressed mainly on CD 4 T cells , whereas CD 86 was expressed on CD 8 T cells , and these two populations were associated with particular clinical features . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OBJECTIVE : To examine whether rheumatoid synovial fluid ( SF ) inhibits dendritic cell ( DC ) expression of the CD 80 and CD 86 costimulator molecules and contributes to SF T lymphocyte hyporesponsiveness . ^^^ In the presence of SF , DC clustered with autologous T lymphocytes showed decreased CD 80 and CD 86 expression , and variable CD80 / CD86 decreases were observed on DC clustered with allogeneic T lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Scurfy T cells also exhibit strong up regulation of cell surface Ags indicative of in vivo activation , including CD 69 , CD 25 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They expressed markers related to DCs ( CD1a , CD11c , CD 32 and CD 83 ) , costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , human leucocyte antigen ( HLA ) class 1 and 2 molecules and inducible nitric oxide ( NO ) synthase ( NOS 2 ) , and lacked lymphocyte and monocyte markers ( CD 3 , CD 19 , CD 20 , CD 56 and CD 14 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment with BPD MA and light reduced DC levels of major histocompatibility ( MHC ) Class 1 and 2 antigens , intercellular adhesion molecule 1 ( ICAM 1 , CD 54 ) , the costimulatory B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules , leucocyte common antigen CD 45 , the apoptosis regulating Fas ( CD 95 ) receptor and the integrin CD11c . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Functional expression of CD 80 and CD 86 allows immunogenicity of malignant B cells from non Hodgkin ' s lymphomas . ^^^ Among the 70 samples of malignant B cells included , four patterns of expression of the costimulatory molecules CD 80 and CD 86 were distinguished ( + / + , + / , / + and / ) . ^^^ In two thirds of the cases , CD 80 , CD 86 , or both were expressed . ^^^ Regardless of the level of expression of CD 80 and CD 86 , significant proliferation was induced in the responder cells . ^^^ The addition of monoclonal antibodies directed against CD 80 and CD 86 at the beginning of MLR almost completely inhibited this proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CBDC expressed CD 4 , CD11a , CD 18 , CD45RA , CD 50 , CD 54 , and CD 123 , but showed no expression of CD1a , CD11c , CD 33 , CD 40 , CD45R0 , CD 80 , CD 83 , and CD 86 and only limited expression of CD 58 , CD 102 , and CD 116 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DNA immunization with the myelin minigene for PLP altered expression of B7 . 1 ( CD 80 ) , and B7 . 2 ( CD 86 ) on APCs in the spleen . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
T cell activation requires binding of the T cell receptor to the major histocompatibility molecule peptide complex in the presence of adhesion and / or costimulatory molecules such as B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , ICAM 1 ( CD 54 ) , and LFA 3 [ corrected ] . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interaction of CD 28 with CD 80 or CD 86 molecules on APC initiates a costimulatory or second signal within the T cell which augments and sustains T cell activation initiated through the TCR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both molecules bind to the same ligands , B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) but have antagonistic functions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CD1a+ cells reacted with antibodies against protein S 100 , HLA DR , the Lag antigen , CD 4 , CD11a , CD11b , CD 18 , CD 25 , CD 40 , CD 54 , CD 80 and CD 86 . ^^^ Both allogenic and antigen specific T cell proliferation stimulated by antigen presenting lymph cells were strongly inhibited by adding anti CD 80 and anti CD 86 antibodies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To determine whether antilymphocyte Abs to T cell costimulatory molecules are generated in patients with autoimmune diseases and , if they exist , to clarify the mechanism of their production and pathological roles , we investigated the presence of autoantibodies to CTLA 4 ( CD 152 ) , CD 28 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) in serum samples obtained from patients with various autoimmune diseases and from normal subjects using recombinant fusion proteins . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In accordance with the characteristics of in vivo LC , we show that LC obtained from human monocytes in vitro in the presence of TGF beta 1 1 ) express almost exclusively intracellular class 2 Ags , low CD 80 , and no CD 83 and CD 86 Ags and 2 ) down regulate TNF RI ( p 55 ) and do not produce IL 10 after stimulation , in contrast to dermal dendritic cells and monocyte derived dendritic cells . ^^^ Surprisingly , while LC exhibit E cadherin down regulation upon exposure to TNF alpha and IL 1 , TGF beta 1 prevents the final LC maturation in response to TNF alpha , IL 1 , and LPS with respect to Class 2 CD 80 , CD 86 , and CD 83 Ag expression , loss of FITC dextran uptake , production of IL 12 , and Ag presentation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cells expressing CD 4 , CD 8 , CD 18 , CD49d / CD29 , CD 44 , CD 54 , CD 80 , CD 86 , CD 106 , CD11b / CD18 or DNA breaks were stained in the pancreases of female or male scid / scid mice after adoptive transfer of lymphocytes from older than 12 week female nonobese diabetic ( NOD ) or Balb / c mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Monocyte / macrophage markers included CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 54 , CD 71 , CD 80 and CD 86 and HLA class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Bladder cancer immunogenicity : expression of CD 80 and CD 86 is insufficient to allow primary CD4+ T cell activation in vitro . ^^^ This in vitro study examined the potential of such a strategy by transfection of the prototypical TCC line J 82 with the important costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ Transfected J 82 cells expressed CD 80 or CD 86 at levels comparable to an antigen presenting B cell line . ^^^ Furthermore , functional surface expression of CD 80 and CD 86 was demonstrated in a mitogen dependent assay of costimulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Epidermal cells were prepared from these earlobes 24 h later , and the levels of MHC class 2 , CD 54 , CD 80 , and CD 86 on these cells were analyzed by flow cytometry . ^^^ As compared with untreated earlobes , the levels of MHC class 2 and CD 86 on LC were markedly augmented and those of CD 54 and CD 80 were slightly elevated in earlobes treated with TCSA / UVA . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Only a few DC in the uveal tract were very weakly reactive for mAbs which recognise B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , beta 2 integrin ( mAb N 418 ) , and multivesicular bodies associated with antigen presentation ( mAb M 342 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : We investigated surface expression of the costimulatory molecules CD 58 ( LFA 3 ) , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) by using flow cytometry , confocal microscopy , and vectorial biotinylation . ^^^ Antibodies specific for CD 58 , CD 80 , and CD 86 were used in blocking experiments to assess the role of these molecules in providing a costimulatory signal to CD 4 ( + ) T cells by IECs . ^^^ RESULTS : CD 58 , but not CD 80 or CD 86 , was observed to be expressed constitutively on both native IECs and in the IEC lines T 84 and HT 29 . ^^^ Antibodies against CD 58 , but not CD 80 or CD 86 , inhibited the stimulation of CD 4 ( + ) T cell proliferation mediated by IECs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast to results described with B type chronic lymphocytic leukemia , also characterized by high levels of circulating TNF , none of the tested samples showed a regulation of CD 80 , CD 86 , CD 27 and CD 70 in response to TNF . ^^^ After culture with human CD40L transfected L cells , an up regulation of CD 80 , CD 86 and CD 70 expression was observed , while TNF addition in this model did not influence these changes . ^^^ Taken together , this data demonstrates that in vitro expression of costimulatory molecules such as CD 80 , CD 86 , CD 27 and CD 70 , which are implicated in the anti tumoral response , can be regulated by CD 40 ligand but not by TNF . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have studied the immune response of the host testis against experimental testicular teratoma in mouse by localizing adhesion molecules ( CD 106 , CD 54 , CD49d / CD29 , CD 44 , CD 18 , CD 8 and CD 4 ) , cytokines ( IL 2 , IL 4 , IL 6 , IL 10 and IL 12 ) , T cell costimulators ( CD 80 , CD 86 ) and the lipid antigen presenting molecule CD1d in the testis of 129 / SvJ mice with and without experimental testicular teratoma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of LFA 1 , ICAM 1 , CD 80 and CD 86 molecules in lupus patients : implication for immunotherapy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The supernatant factor also exhibits a marked inhibitory effect on the expression of the costimulatory molecules , CD 80 and CD 86 , by APC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have developed several in vivo models to study allergen specific T cell and B cell function and their relevance to allergic airway hyperresponsiveness ( AHR ) , focusing on the role of the costimulatory molecules CD 80 and CD 86 . ^^^ Treatment of mice with anti CD 86 , but not anti CD 80 , significantly inhibited increased serum levels of ovalbumin ( OA ) specific IgE and IgG 1 , airway eosinophilia , and AHR both after 10 d of OA aerosol exposure ( in the absence of adjuvant ) and after intraperitoneal sensitization followed by repeated airway challenges . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
As the B7 / CD28 costimulatory pathway has been shown to influence the differentiation of Th cell subsets , we investigated the contribution of the B 7 molecules CD 80 and CD 86 to Th1 / Th2 cytokine and immunoglobulin isotype profiles and to the development of a protective immune response to malaria in NIH mice infected with Plasmodium chabaudi . ^^^ Moreover , combined CD80 / CD86 blockade by using anti CD 80 and anti CD 86 monoclonal antibodies raised IFN gamma production over that seen with CD 86 blockade alone , with augmentation of this Th 1 associated cytokine reducing levels of peak primary parasitaemia . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Since these two receptors use the same ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , the co ordinate timing of CD 28 and CTLA 4 expression has a major impact on the regulation of immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The unique functional characteristics of microvascular LSECs together with a unique phenotype ( CD 4 ( + ) , CD11b+ , CD11c+ , CD 80 ( + ) , CD 86 ( + ) ) make these cells different from macrovascular endothelial cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To verify whether alveolar macrophages ( AM ) of patients with hypersensitivity pneumonitis ( HP ) increase their antigen presenting capacity by upregulating the expression of B 7 costimulatory molecules ( CD 80 , CD 86 ) , and whether a viral infection enhances this expression whereas cigarette smoking abrogates it , we performed bronchoalveolar lavage ( BAL ) on 18 patients with HP ; 10 asymptomatic , virus exposed subjects ( AS ) ; 18 nonsmokers ; and 12 smokers . ^^^ Expression of CD 80 and CD 86 on AM , and of CD 28 and CTLA 4 on T cells , was evaluated . ^^^ In vitro virus infection upregulated CD 80 and CD 86 expression in AM of normal nonsmoking subjects but not on those of smokers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Monocyte derived DC transiently incubated with the anti CD 43 mAb , MEM 59 , or with F ( ab ' ) 2 fragments , but not with monovalent Fab fragments or control IgG , 24 h later showed increased levels of membrane HLA DR , CD 54 , CD 40 , CD 80 , CD 86 , and CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression and contribution of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in the early immune response to Leishmania major infection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Compared with mature recirculating follicular B cells , they express several characteristics of previous antigenic experience , including higher levels of B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) when freshly isolated and following in vitro stimulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , no up regulation of CD 80 , CD 86 , CD45RC and OX 40 ( CD 134 ) was observed on T cells during T T presentation or subsequent antigenic restimulation of anergic T cells in the presence of professional APC , whereas increased expression of these molecules was observed during professional APC T presentation of non anergic T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OBJECTIVE : We studied whether this difference may be correlated to increased expression of membrane costimulatory molecules , such as the B 7 molecules ( CD 80 and CD 86 ) . ^^^ RESULTS : Allergic individuals had a significantly higher proportion of AMs expressing the CD 80 molecule than control subjects ( 28 . 5 % + / 14 . 8 % vs 1 . 4 % + / 1 . 2 % ; P < . 001 ) , whereas no difference was observed in CD 86 expression ( 2 . 0 % + / 2 . 3 % vs 1 . 1 % + / 0 . 6 ; P > . 1 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
MP prevented LPS induced DC maturation as assessed by the inhibition of CD 86 , CD 80 and CD 54 up regulation , CD 83 induction and production of TNF alpha , IL 6 , and IL 12 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , neither CD 80 nor CD 86 nor CD 102 was found on the surface , and these costimulatory molecules could not be induced by any cytokines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Enhanced expression of CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 and their ligands CD 28 and CD 154 in fulminant hepatic failure . ^^^ To define a possible role for changes in the regulation of antigen presentation in fulminant hepatic failure ( FHF ) , we studied the expression of co stimulatory molecules CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 along with their ligands CD 28 and CD 154 . ^^^ In FHF , immunoreactivity for CD 80 and CD 86 was observed on significantly higher numbers of cells , including SECs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) costimulatory molecules in tumor cells has proven to be an efficient way to enhance their immunogenicity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory molecules CD 80 and / or CD 86 by a Kaposi ' s sarcoma tumor cell line induces differential T cell activation and proliferation . ^^^ Besides the first antigen specific signal , the second costimulatory signal involves CD 80 and CD 86 expressed by the antigen presenting cell . ^^^ We previously observed that tumor cells in Kaposi ' s sarcoma ( a common AIDS related cutaneous neoplasm ) completely lack both CD 80 and CD 86 , and these tumor cells fail to stimulate T cell proliferation . ^^^ Tumor cells that were either singly positive for either CD 80 or CD 86 , as well as a double positive cell line , were examined for their ability to induce T cell activation , T cell proliferation , and cytokine production profiles . ^^^ Compared to the parental double negative tumor cell line , the CD 80 positive cells , but not the CD 86 positive tumor cells , induced significant T cell activation and proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The ligands or counterreceptors for these two proteins are the B 7 family members , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Two colour flow cytometry revealed a similar increase in donor derived H 2Kb+ / I Ab+ cells , and in the incidence of donor leucocytes expressing CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The interaction between the T cell surface molecules CD 28 and costimulatory molecules of antigen presenting cells ( CD 80 or CD 86 ) is the most important point of the T helper cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vitro derived DC were infected with BCG , which induced their maturation , as shown by the increased expression of MHC class 2 antigens , CD 80 and CD 86 co stimulatory molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
First , we found that the addition of IFN beta at the initiation of the culture did not modify DC morphology but caused a reproducible and statistically significant upregulation of HLA DR , CD 86 , and CD 80 surface expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These mutations in CTLA 4 ablated binding to its natural ligands CD 80 and CD 86 , whereas binding to a conformation dependent anti CTLA 4 monoclonal antibody showed that the 5 domain framework remained correctly folded . ^^^ CTLA 4 displayed on phage bound specifically to immobilized CD 80 Ig and CD 86 Ig and in one step panning enriched 5 , 000 to 2 , 600 fold respectively over wild type phage . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we examined whether SCC that undergo transformation and development in the absence of specific immunity exhibit differences in tumorigenicity that relate to differences in expression of CD 80 , CD 86 or MHC class 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) antigens on house dust mite specific T cells in atopic disease function through T T cell interactions . ^^^ BACKGROUND : CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) play an important role in antigen presentation to effector cells . ^^^ However , the functional role of CD 80 and CD 86 expressed on allergen specific T cells in atopic diseases has not yet been clarified . ^^^ OBJECTIVE : We sought to determine the functional role of CD 80 and CD 86 expressed on allergen specific T cells in atopic diseases . ^^^ METHODS : We assayed the expression of CD 80 and CD 86 on allergen specific T cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae crude ( Der f c ) antigen , 1 of the major allergens causing house dust mite allergy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : We determined the in situ phenotype of mature CD83+ TiDCs using monoclonal antibodies to known activation molecules ( CD 86 [ B7 . 2 ] , CD 80 [ B7 . 1 ] , CD 40 , CD 54 , CD1a and HLA DR ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , anti CD 40 mAb was found to enhance the expression of the B 7 2 ( CD 86 ) and , to a lesser extent , the B 7 1 ( CD 80 ) costimulatory molecules on B220+ cells ( i . e . , B cells ) , and B 7 2 and , to a lesser extent , B 7 1 molecules played an important role in the potentiating effect of anti CD 40 mAb for CTL generation by tumor bearer splenic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendritic cells generated in mice treated with Flt3L alone or in combination with CD40L were equally potent in stimulating allogeneic T cells and expressed similar levels of MHC class 2 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Collectively , these results demonstrate the importance of costimulation for naive CD8+ T cell activation , suggest that CD 80 and CD 86 can mediate opposing effects , possibly due to differential interaction with CD 152 and CD 28 , and indicate differences in the sensitivity of immature vs mature CD8+ T cells to the induction of nonresponsiveness following costimulation deficient Ag presentation . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Significant differences in surface antigen expression DC vs M were recognizable for MHC class 2 , CD 86 , CD 80 , CD1a , CD 40 and ICAM 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We sought to enhance the protective immune response induced during Mycobacterium bovis BCG infection by administering an antibody that blocks the interaction of CTLA 4 with its ligands , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These are provided by costimulatory molecules , including B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) and intercellular adhesive molecule 1 ( ICAM 1 ; CD 54 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To examine if the muscle fibers in patients with inflammatory myopathies have the potential to behave as antigen presenting cells ( APCs ) , we investigated the expression of costimulatory molecules BB 1 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) , and their counterreceptors , CD 28 or CTLA 4 ( CD 152 ) , in the muscle biopsies of patients with polymyositis ( PM ) , PM associated with human immunodeficiency virus infection ( HIV PM ) , sporadic inclusion body myositis ( s IBM ) , dermatomyositis ( DM ) , and normal or disease controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , the expression of CD 80 and CD 86 , which both decreased in the stress burdened mice , was restored to the normal level by TJ 41 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although the promyelocytic leukemia line HL 60 was refractory to cytokine differentiation , CI treatment induced near uniform overnight expression of CD 83 , CD 80 ( B7 . 1 ) , and CD 86 ( B 7 . 2 ) , as well as additional characteristics of mature DC . ^^^ In parallel studies , CD 34 ( pos ) cells cultured from normal donor bone marrow developed marked DC like morphology after overnight treatment with either rhCD40L or CI , but only CI simultaneously induced upregulation of CD 83 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B7 . 2 ( CD 86 ) but not B7 . 1 ( CD 80 ) costimulation is required for the induction of low dose oral tolerance . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , expressions of S 100 , CD 25 , CD 68 , CD 80 , CD 86 , and Fas ligand were identified on these cells by immunocytochemical staining . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : Forty six patients with MS and 29 healthy individuals were analyzed by direct 2 color immunofluorescence flow cytometry for expression of CD 80 , CD 86 , CD 28 and CTLA 4 on T and B cells and monocytes . ^^^ Marked increase in the expression of CD 80 and CD 86 was seen on both CD4+ and CD8+ T cells from an MS patient with rapidly progressing disease . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The accessory function of antigen presenting cells depends on the presence of a number of costimulatory molecules , including members of the B 7 family ( CD 80 and CD 86 ) and the CD 5 coligand CD 72 . ^^^ Although normal alveolar macrophages ( AMs ) did not bear or bore low levels of costimulatory molecules , AMs from sarcoid patients with CD 4 T cell alveolitis upmodulated CD 80 , CD 86 , and CD 72 and expressed high levels of interleukin ( IL ) 15 ; lymphocytes accounting for T cell alveolitis expressed Th 1 type cytokines [ interferon ( IFN ) gamma and / or IL 2 ] and bore high levels of CD 5 and CD 28 but not of CD 152 molecules . ^^^ In vitro stimulation of AMs with Th 1 related cytokines ( IL 15 and IFN gamma ) upregulated the expression of CD 80 and CD 86 molecules . ^^^ The hypothesis that CD 80 and CD 86 molecules regulate the sarcoid T cell response was confirmed by the evidence that AMs induced a strong proliferation of T cells that was inhibited by pretreatment with CD 80 and CD 86 monoclonal antibodies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recombinant IL 16 was found to up regulate CD 25 and CD 80 but to down regulate CD 4 and CD 86 surface expression in MDM cultures . ^^^ However , no change could be observed on the level of CD 4 , CD 80 and CD 86 expression in IL 16 stimulated MDDC , although a significant up regulation of CD 25 and CD 83 was consistently detected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here we show that peripheral blood eosinophils from hypereosinophilic patients could express membrane CD 86 but not CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , human ECs may be limited in their ability to effectively activate CD 4 ( + ) T cells , because they do not express members of the B 7 family ( CD 80 and CD 86 ) of costimulatory molecules . ^^^ By reverse transcriptase polymerase chain reaction , Western blot , and FACS ( ( R ) ) analysis , we could not detect the expression of CD 80 and CD 86 on activated ECs and found minimal expression on purified CD 4 ( + ) T cells . ^^^ In contrast , both CD 80 and CD 86 were expressed in allogeneic CD 4 ( + ) T cell EC cocultures . ^^^ Expression of CD 86 peaked at early times between 12 and 24 h after coculture , whereas CD 80 was not expressed until 72 h . ^^^ Addition of anti CD 86 but not anti CD 80 monoclonal antibodies to cocultures inhibited IL 2 production and the proliferation of CD 4 ( + ) T cells to allogeneic donor human umbilical vein ECs ( HUVECs ) , as well as to skin and lung microvascular ECs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , traf 5 ( / ) B cells showed defects in proliferation and up regulation of various surface molecules , including CD 23 , CD 54 , CD 80 , CD 86 , and Fas in response to CD 40 stimulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Inhibition of the interaction of T cells with CD 86 , but not CD 80 , at the time of exposure to intranasal Ag prevented the development of unresponsiveness , while selective blockade of CTLA 4 had no effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The aim of this study was to demonstrate expression of costimulatory molecules CD 86 and CD 80 in human colonic epithelial cells and assess their functional roles in the activation of T cells in inflamed colonic mucosa . ^^^ METHODS : CD 86 and CD 80 expression was assessed by immunohistochemistry of colonic mucosa , reverse transcription polymerase chain reaction , and flow cytometric analysis of isolated colonic epithelial cells and cell lines . ^^^ RESULTS : CD 86 and CD 80 messenger RNA was detected in isolated colonic epithelial cells from normal and inflamed mucosa of patients with ulcerative colitis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment of purified CD 33 ( + ) CML cells from 15 patients with calcium ionophore ( CI ) consistently resulted in de novo expression of the costimulatory molecules CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , CD 40 and the DC specific activation marker CD 83 , as well as marked up regulation of MHC class 1 and 2 molecules and the adhesion molecule CD 54 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Tumor cell lines also expressed epidermal growth factor ( EGF ) receptor , MHC class 1 , and CD 95 ( Fas ) but did not uniformly express other important lymphoid regulatory molecules such as CD 80 , CD 86 , or interleukin ( IL ) 2 receptor components . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , in vitro propagated DC were transduced using an adenoviral ( Ad ) vector to express the gene encoding cytotoxic T lymphocyte antigen 4 immunoglobulin ( CTLA4lg ) , which blocks interaction of CD 80 and CD 86 on DC with CD 28 on T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In particular , S . gordonii dose dependently up regulated expression of membrane molecules ( MHC 1 and 2 , CD 80 , CD 86 , CD 54 , CD 40 , CD 83 ) and reduced both phagocytic and endocytic activities . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , CD 40 ligation , but not TNF alpha or lipopolysaccharide treatment , of activated , migratory DC that had migrated from skin explants resulted in a 2 . 5 fold increase of surface expression of FK without significant alterations of expression of CD 80 , CD 86 , CD 54 or MHC class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : CD 40 CD40L interactions induced the expression of the adhesion molecules VCAM 1 and E selectin and the costimulatory molecule CD 80 but not CD 86 ( B 7 2 ) on the MECs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The NEC and AEC also expressed the costimulatory molecules CD 80 and CD 86 as determined with flow cytometry , the reverse transcription polymerase chain reaction for RNA , and immunohistochemistry , and T cell proliferation could be blocked by treating NEC and AEC with anti CD 80 and anti CD 86 antibodies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They expressed a set of DC associated markers , such as MHC class 2 , CD1a , CD 4 , CD11a , CD 40 , CD 58 , CD 80 , CD 83 , CD 86 , and CXCR 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In parallel , CT induced up regulation of CD 80 and CD 86 on these Flt3L expanded DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Whereas the virus infected T cells expressed higher levels of HLA DR , CD 25 , CD 80 , and CD 86 Ags than apoptosis resistant PHA blasts , the T cell apoptosis was enhanced by addition of exogenous IL 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 ( CD 152 ) , a receptor for the B 7 costimulatory molecules ( CD 80 and CD 86 ) , is considered a fundamental regulator of T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both proteins are ligands for the costimulatory molecules CD 80 and CD 86 expressed by activated B cells , macrophages , and dendritic cells . ^^^ A fusion protein comprising the CTLA 4 extracellular domain joined to a human immunoglobulin heavy chain constant region ( CTLA4Ig ) binds CD 80 and CD 86 with high affinity and inhibits CD80 / CD86 dependent immune responses in vitro and in vivo . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Human fibroblasts transduced with CD 80 or CD 86 efficiently trans costimulate CD4+ and CD8+ T lymphocytes in HLA restricted reactions : implications for immune augmentation cancer therapy and autoimmunity . ^^^ We have evaluated the efficiency of CD 80 and CD 86 mediated trans costimulation in the activation of human CD8+ and CD4+ T lymphocytes in MHC class 1 and class 2 restricted lymphoproliferation reactions . ^^^ Our studies demonstrate that the efficiency of CD 80 or CD 86 mediated trans costimulation of purified human CD8+ and CD4+ T lymphocytes is comparable to cis costimulation under defined conditions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The monocytic cells did not account for this difference , as CD 80 and CD 86 expressions were similar , and coculturing PB T cells with alveolar macrophages resulted in no difference in IFN gamma production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The DC phenotype was defined as upmodulation of the costimulatory molecules CD 80 and CD 86 and the expression of CD1a or CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 40 , CD 80 , and CD 86 antigens were constitutively expressed on 3 . 2 % + / 4 . 5 % , 0 % , and 1 . 8 % + / 1 . 2 % CD 34 ( + ) blood cells , respectively . ^^^ After incubation for 24 hours with TNF alpha CD 34 ( + ) CD 40 ( + ) blood cells expressed only myeloid markers and contained less than 5 % CD 86 ( + ) and CD 80 ( + ) cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Human T cell response to L 14 pig cell line transfected with human ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 interactions with either CD 80 or CD 86 are sufficient to induce allergic airway inflammation in mice . ^^^ In the present study , the importance of CD 28 as well as the individual roles of CD 80 and CD 86 were examined in this system using wild type and CD 28 knockout ( KO ) mice . ^^^ We next determined by polymerase chain reaction and flow cytometry that CD 80 and CD 86 molecules are constitutively expressed in the naive murine lung and on eosinophils in the allergic lung , suggesting a potential important role for both ligands in the development of asthma . ^^^ Combined anti CD80 / anti CD 86 treatment throughout the antigen challenge period fully blocked the development of allergic airways , whereas a partial reduction was observed in mice treated with either anti CD 80 or anti CD 86 antibody alone . ^^^ Therefore , signaling through either CD 80 or CD 86 is sufficient to generate a partial local allergic response , whereas CD 86 costimulation is essential to induce systemic allergic ( IgE ) reactions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Antibodies against muscarinic M 3 receptor and expression of costimulatory molecules ( CD 80 and CD 86 ) by ductal epithelial cells may play a role in pathogenesis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NK cell triggering by the human costimulatory molecules CD 80 and CD 86 . ^^^ Recently , it has been described that murine NK cells are triggered by the costimulatory molecules CD 80 , CD 86 , and CD 40 . ^^^ Using NK cell lines derived from PBMC as effectors , we demonstrate that the human CD 80 and CD 86 gene products can function as triggering molecules for NK cell mediated cytotoxicity . ^^^ Expression of human CD 80 or CD 86 molecules in murine B16 . ^^^ Triggering of human NK cells by CD 80 and CD 86 appeared to be independent of CD 28 and CTLA 4 , at least as determined by the reagents used in the present study , because the expression of these molecules could not be detected on the NK cell lines by either flow cytometry or in redirected lysis assays . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Among these accessory molecules , interactions between CD28 / CTLA 4 with B 7 family members ( CD 80 and CD 86 ) and CD 40 with CD 40 ligand ( CD40L ) play a decisive role in regulating the progression of balanced immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expression in multiple sclerosis patients : clinical subtype specific variation in peripheral monocytes and B cells and lack of modulation by high dose methylprednisolone . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The role of CD 80 and CD 86 in enhancing CD 8 ( + ) cell suppression of HIV replication . ^^^ In the present study CD 8 ( + ) cells exposed to purified forms of both the CD 80 and the CD 86 molecules during stimulation with anti CD 3 antibodies ( Ab ) had a greater ability to suppress HIV replication than CD 8 ( + ) cells exposed to anti CD 3 Ab alone . ^^^ The addition of anti CD 86 blocking Ab , but not anti CD 80 blocking Ab , to Mphi decreased their ability to enhance CD 8 ( + ) cell suppression of HIV replication . ^^^ Moreover , anti CD 86 Ab and not anti CD 80 Ab blocked the production of IL 2 by CD 8 ( + ) cells stimulated in the presence of Mphi . ^^^ The difference in use of CD 86 over CD 80 molecules on Mphi by CD 8 ( + ) cells mediating the antiviral suppressing activity most likely results from a higher number of Mphi expressing the CD 86 molecule compared with the CD 80 molecule . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
P ExA also inhibited IFN gamma induced cellular events : ( 1 ) expression of costimulatory molecules on monocytes ( CD 80 , CD 86 , ICAM 1 , HLA DR ) ; ( 2 ) cytotoxic activity of NK cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dual flow cytometry analysis performed weekly demonstrated increasing co expression of CD1a or CD 83 with HLA DR , CD 80 , CD 86 , IL 2R alpha , beta , and gamma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These are : ( 1 ) low expression of the costimulatory molecules CD 40 , CD 80 , and CD 86 ; ( 2 ) poor expression of the differentiation marker CD 83 and no CD1a ; and ( 3 ) good capacity to incorporate dextran . ^^^ Although lung DC express low levels of CD 80 and CD 86 , the important role of these costimulatory molecules in inducing high MLR was demonstrated by using blocking antibodies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
As the co stimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) play an integral role in the activation of T cells by antigen presenting cells ( APC ) we investigated the surface expression of B 7 1 and B 7 2 on colonic macrophages from normal and IBD mucosa . ^^^ RESULTS : The expression of B 7 1 ( CD 80 ) ( 9 . 2 % + / 4 . 2 % ) and B 7 2 ( CD 86 ) ( 15 . 1 % + / 7 . 3 % ) was low on colonic macrophages from normal mucosa , indicating only a low antigen presenting potential . ^^^ However , on macrophages from IBD colon there was a significant increase in the expression of co stimulatory molecules ( CD 80 , 33 . 8 % + / 8 . 9 % , P = 0 . 00005 vs . control ; CD 86 , 39 . 9 % + / 8 . 8 % , P = 0 . 00002 ) . ^^^ There was no significant difference between CD and UC in the expression of CD 80 ( CD , 31 . 3 % + / 6 . 7 % ; UC , 34 . 4 % + / 13 . 3 % ) and CD 86 ( CD , 41 . 9 % + / 3 . 8 % ; UC , 35 . 6 % + / 13 . 8 % ) . ^^^ CONCLUSION : Colonic macrophages from normal mucosa rarely express the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) as activation markers that are present at low density on the cell surface was analyzed in comparison to CD 22 ( B cells ) and CD 4 ( T cell subset ) , that show high staining intensities . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
UVB stimulated the maturation of the former LC subset as demonstrated by enhanced up regulation of CD 80 , CD 86 , CD 54 , CD 40 , and CD 83 and reduced CD1a expression in comparison with unirradiated controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Screening of various soluble factors revealed that IL 4 induces phenotypic maturation of XS 52 ( as evaluated by enhanced expression of class 2 , CD 40 , CD 80 , CD 86 , CD11c , and loss of expression of CD 14 ) in a time dependent manner . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Addition of TNF alpha or LPS induced both normal and CMML Mo DC to express prominent dendritic processes , the CMRF44+ and CD83+ antigens and high levels of HLA DR , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Characteristic of dendritic cell maturation , R 848 treatment induces cell surface expression of CD 83 and increases cell surface expression of CD 80 , CD 86 , CD 40 , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Peripheral blood antigen presenting cells from African Americans exhibit increased CD 80 and CD 86 expression . ^^^ The expression of both B 7 costimulatory molecules was significantly higher on the cells from AA subjects compared with cells from CS subjects ( CD 80 , P < 0 . 05 ; CD 86 , P < 0 . 05 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After 7 days , when returned to medium containing human serum without cytokines , some monocyte derived dendritic cells ( MDDC ) became adherent , but retained the costimulatory markers CD 80 and CD 86 and continued to express CD 83 and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , the minority ( 10 30 % ) of DC clustering with T lymphocytes showed antigen specific up regulation of the CD 40 , CD 80 and CD 86 costimulator molecules , whereas the non clustered DC ( 70 90 % ) had less up regulation than control DC cultured alone and did not respond to antigen specific triggering . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , expression of the co stimulatory ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , as well as the receptors CD 28 and CTLA 4 , were quantitated in central nervous system ( CNS ) tissues from mice at various stages of EAE . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Glycosyl phosphatidylinositol ( GPI ) modified variants of the murine costimulators B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , designated B 7 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We now show that infection by rAd enhances the immunostimulatory capacity of immature human monocyte derived DC through the upregulation of the costimulatory molecules CD 80 , CD 86 , and CD 40 and the major histocompatibility complex class 1 and 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
FACS analysis of CD2+ and CD 2 monocytes revealed no significant difference in the expression of adhesion molecules ( CD11a / b / c ) , class 2 Ags ( HLA DR , DQ , DP ) , myeloid Ags ( CD 13 , CD 14 , CD 33 ) , or costimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells were characterized by the production of IL 10 and TGF beta 2 , but not IL 12 , IL 1alpha , or TNF alpha , and they expressed CD 14 , CD 16 , and CD 54 , but not HLA DR , CD 80 , CD 86 , CD11a , CD11b , or CD 25 cell surface Ags . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Essential role for both CD 80 and CD 86 costimulation , but not CD 40 interactions , in allergen induced Th 2 cytokine production from asthmatic bronchial tissue : role for alphabeta , but not gammadelta , T cells . ^^^ CD 80 and CD 86 interact with CD 28 and deliver costimulatory signals required for T cell activation . ^^^ Allergen induced IL 5 and IL 13 production by the asthmatic tissue was inhibited by anti CD 80 and , to a lesser extent , by anti CD 86 mAbs . ^^^ In contrast , the production of these cytokines by PBMCs was not affected by mAbs to CD 80 , was inhibited by anti CD 86 , and was strongly attenuated in the presence of both Abs . ^^^ Collectively , these findings suggest that allergen specific alphabeta T cells are resident in asthmatic bronchial tissue and demonstrate that costimulation by both CD 80 and CD 86 is essential for allergen induced cytokine production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In combination with Tumor necrosis factor alpha , ATP increased the expression of the DC surface markers CD 80 , CD 83 and CD 86 indicating a maturation promoting effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After 5 days of culture , cell surface expression of MHC class I / II , CD 40 , CD 80 , and CD 86 was analyzed by flow cytometry , and nuclear NF kappaB proteins by electrophoretic mobility shift , antibody supershift , and Western blot assays . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DCs derived from GM CSF [ granulocyte / macrophage colony stimulating factor ] + interleukin ( IL ) 4 cultures expressed high levels of major histocompatibility complex ( MHC ) class 2 , CD 40 , CD 80 , and CD 86 molecules and were efficient stimulators of naive allogeneic T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interaction of CTLA 4 ( CD 152 ) with CD 80 or CD 86 inhibits human T cell activation . ^^^ It is concluded that interaction of CTLA 4 with its functional ligands , CD 80 or CD 86 , can down regulate human T cell responses , probably by intracellular signalling events and independent of CD 28 occupancy . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This effect is due to inhibition of the CD 40 pathway which normally leads to CD 80 and CD 86 up regulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Development of human T cell leukemia virus type 1 transformed tumors in rats following suppression of T cell immunity by CD 80 and CD 86 blockade . ^^^ Four week old WKA / HKm rats were treated with monoclonal antibodies ( MAbs ) to CD 80 and CD 86 and subcutaneously inoculated with syngeneic HTLV 1 infected TARS 1 cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The stimulatory hybrids expressed human leucocyte antigen ( HLA ) class 1 and 2 , and a wide range of surface accessory molecules , including the T cell co stimulatory ligand molecules CD 40 , CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , the expression of which was required for optimal stimulation of T cell responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Adhesion molecule ( CD 54 and CD 106 ) expression was not diminished by IL 10 treatment , and costimulatory molecule ( CD 80 and CD 86 ) expression was augmented by administration of exogenous IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory molecules CD 80 and CD 86 and their receptors CD 28 , CTLA 4 on malignant ascites CD3+ tumour infiltrating lymphocytes ( TIL ) from patients with ovarian and other types of peritoneal carcinomatosis . ^^^ Costimulation of T lymphocytes by the leucocyte surface molecules CD 80 and CD 86 expressed on antigen presenting cells ( APC ) is required for the development of T cell responses . ^^^ The CD 28 and CTLA 4 molecules on T cells serve as receptors for the CD 80 and CD 86 costimulatory antigens . ^^^ We have examined the frequency of expression of CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , CD 28 and CTLA 4 surface antigens on TIL isolated from malignant ascites or peritoneal washings of 26 patients with ovarian carcinoma and five patients with non ovarian peritoneal carcinomatosis . ^^^ Expression of CD 80 and CD 86 antigen was detected by reverse transcription polymerase chain reaction ( RT PCR ) , and by FACS analysis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After a 6 h incubation , phenotypic analysis of control , imiquimod , or R 848 treated LC indicated that such antigen presenting cells were in an `` intermediate ' ' state of maturation ( CD1a ( + ) , HLA DR , DP , DQ ( bright+ ) , CD 40 ( low+ ) , CD 86 ( high+ ) , and CD 80 ( low+ ) ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that an HLA DR+ subclone ( HEL DR+ ) expresses LFA 1 , LFA 3 , ICAM 1 , ICAM 3 , but neither CD 80 nor CD 86 on the surface . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
At day 7 post BLM , 48 % of CD11b+ BAMC displayed features of DC differentiation , as judged by dendritic morphology , expression of class 2 MHC , 33D1 , Factor XIIIa , CD 80 , and CD 86 antigens , and the ability to support a primary allogeneic lymphocyte response ( MLR ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis of surface antigen expression identified that after 5 days of culture the proliferating subpopulation of monocytes expressed CD 14 , CD 13 , CD 33 , CD11b , CD11c , CD 87 , HLA DR , CD45RO , and did not express CD 86 , CD 34 , CD 80 , CD 4 , CD 16 , and CD 56 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypically , CD11c + DC cultured fetal calf serum free homogenously coexpressed the DC specific molecule DEC 205 as well as the costimulatory molecules CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of the costimulator molecules , CD 80 , CD 86 , CD 28 , and CD 152 on lymphocytes from neonates and young children . ^^^ The expression of CD 80 , CD 86 , CD 28 , and CD 152 were examined on peripheral blood lymphocytes from adults , neonates ( cord blood lymphocytes ) and young children ( 2 20 months of age ) . ^^^ There was no difference in the expression of CD 80 or CD 86 between adult and neonatal B cells , either resting or activated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blockade of the interaction of CD 28 with its ligands CD 80 and CD 86 using CTLA 4 Ig has been proposed as a therapy for a number of immune based disorders . ^^^ In vivo treatment with CTLA 4 Ig to block the interaction of CD 28 with CD 80 and CD 86 reduced virus specific cytotoxicity and IFN gamma production by bronchoalveolar lavage fluid CD8+ T lymphocytes in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both cultures expressed class 2 MHC ( HLA DR ) , but the proportion of cells expressing the costimulatory molecules CD 80 and CD 86 was significantly less in cultures that developed from HNSCC patient CD34+ cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
New forms of dog CD 80 and CD 86 transcripts that encode secreted B 7 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The present review focuses on the role of two receptor ligand pairs : CD 28 and cytotoxic T lymphocyte associated antigen 4 , which interact with the B 7 ligands ( CD 80 , CD 86 ) ; and CD 40 , which interacts with the CD40L ( CD 154 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We studied the effect of toremifene on the expression of HLA DR , ICAM 1 , costimulatory molecules CD 80 and CD 86 , and the tumour necrosis factor receptor ( TNF R ) family molecules CD 27 , CD 30 , CD 40 , TNF R 1 , TNF R 2 and Fas ( CD 95 ) on MCF 7 breast cancer and Jurkat T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
One signal is delivered through the TCR by engagement with peptide / MHC complexes , and the second is delivered by interaction between costimulatory molecules such as CD 28 and its ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We quantitatively measured the number of T cells expressing cell surface activation associated molecules ( CD 69 , CD 25 , CD 122 , HLA DR ) and co stimulatory molecules ( CD 28 , CTLA 4 , CD 80 , CD 86 ) , including a Type 2 T cell marker ( CD 30 ) and CD11b , by flow cytometry of skin and peripheral blood . ^^^ CD 86 , CD 80 , CTLA 4 , CD 30 and CD11b were expressed by less than 23 % of the T cell populations from both the epidermis and dermis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Induction of B 7 1 ( CD 80 ) and increased or sustained expression of CD44H , ICAM 1 ( CD 54 ) , and B 7 2 ( CD 86 ) are dependent on the interaction of CD 40 ligand with CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Vaccination with mouse mammary adenocarcinoma cells coexpressing B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) discloses the dominant effect of B 7 1 in the induction of antitumor immunity . ^^^ Nonreplicating TS / A mammary adenocarcinoma cells expressing B 7 2 ( CD 86 ) ( TS / A 2 ) are more immunogenic than those expressing B 7 1 ( CD 80 ) ( TS / A 1 ) , indicating that B 7 1 and B 7 2 display nonredundant costimulatory effects in inducing antitumor responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The Th 2 skewing of the developing immune response is explained by the effects of PDE inhibitors on several factors contributing to T cell priming : the cytokine milieu ; the type of costimulatory signal , i . e . , up regulation of CD 86 and down regulation of CD 80 ; and the Ag avidity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The surface molecules CD 40 , CD 58 , CD 80 , CD 86 , CD 54 , and MHC class 1 molecules were up regulated in B CLL cells , whereas CD 95 expression was not influenced by CpG ODN stimulation . ^^^ Costimulation with CpG ODN and CD40LF resulted in further up regulation of CD 58 , CD 80 , CD 86 , and MHC class 1 molecules . ^^^ This effect was completely inhibited by a combination of anti CD 80 and anti CD 86 monoclonal antibody . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Further analysis of the FITClo cells from the DMBA treated mice demonstrated that these cells had reduced levels of CD 80 expression , had substantially reduced levels of CD 86 expression and performed poorly as co stimulator cells in an anti CD 3 mediated proliferative assay . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The co stimulatory signal provided by the interaction between CD 28 and its ligands , CD 80 and CD 86 , is critical for T cell activation . ^^^ In the present study , we examined the influence of anti allergic drugs on co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expression in normal mouse splenocytes by flow cytometry . ^^^ Treatment of BALB / c mice with azelastine ( AZ ) at a dose of 50 . 0 micrograms / kg / day , which is the most effective therapeutic dose , for two weeks scarcely affected the expression of co stimulatory molecules CD 40 , CD 80 and CD 86 on splenocytes , whereas a three week treatment strongly suppressed the expression of these molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , the CD40L dependent maturation of DCs is inhibited by MV infection , as demonstrated by CD 25 , CD 69 , CD 71 , CD 40 , CD 80 , CD 86 , and CD 83 expression down regulation . ^^^ In such DC PBL cocultures , inhibition of CD 80 and CD 86 expression on DCs was shown to require both MV replication and CD 40 triggering . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are glycoproteins expressed on antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cyclosporine A was also the most effective drug downregulating the expression of accessory molecules ( CD 54 , CD 58 , CD 80 and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It is well known that tumor cells that express B 7 molecules can elicit antitumor immunity , but little is known regarding which B 7 molecule , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) , can do so more efficiently . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Professional APC are characterized by their ability to present peptide via HLA class 2 in the presence of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Addition of 1 , 25 ( OH ) 2D3 during LPS induced maturation maintains the immature DC phenotype characterized by high mannose receptor and low CD 83 expression and markedly inhibits up regulation of the costimulatory molecules CD 40 , CD 80 , and CD 86 and of class 2 MHC molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It was found that NOD macrophages , dendritic cells , and T cells , but not B cells , expressed lower basal levels of CD 86 , but not CD 80 , CD 28 , or CD 40 , compared with C57BL / 6 and BALB / c . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cells grew and differentiated into DCs as measured by a time dependent upregulation of cell surface antigens major histocompatibility complex class 2 , CD1a , CD 80 , CD 86 , CD 40 , and CD 4 , so that 40 % + / 9 % ( n = 6 ) of cells in culture at day 15 were CD1a+CD14 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The DC population thus obtained had a typical dendritic morphology and expressed DC surface markers , such as HLA class 2 , CD1a , CD11c , CD 54 , CD 80 and CD 86 , but not CD 14 for a period of up to three weeks in culture . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BACKGROUND : B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , which are costimulatory molecules in T cell activation , play important roles in the differentiation of TH 1 or TH 2 phenotypes . ^^^ After in vitro stimulation , the expression of CD 80 and CD 86 on CD19+ cells was analysed by two colour flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , we tested the expression of CD 54 , CD 58 , CD 80 , and CD 86 on AEC 2 and evaluated their accessory function ( AF ) in cell culture using a coculture of interleukin 2 ( IL 2 ) , releasing Jurkat cells and AEC 2 . ^^^ AEC 2 also expressed CD 80 and CD 86 ( 38 % [ 0 77 % ] and 40 % [ 4 68 % ] , respectively ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) have complementary roles in mediating allergic pulmonary inflammation and airway hyperresponsiveness . ^^^ We examined the roles of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in a model of allergic pulmonary inflammation and airway hyperresponsiveness ( AHR ) by using mice with germline deletions of the B 7 1 and / or B 7 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Genetically modified tumors expressing various cytokines , major histocompatibility complex ( MHC ) molecules , or costimulatory molecules such as B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) can induce tumor specific immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We determined B lymphocytes ( CD 40 ( + ) ) , T lymphocytes ( CD 3 ( + ) ) , T helper ( CD 4 ( + ) ) , and T suppressor ( CD 8 ( + ) ) cells and the expression of costimulatory signals B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) on B cells and their counter receptors CD 28 and CTLA 4 on T cells by fluorescence activated cell sorting analysis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Immunophenotyping showed a low expression of CD 80 and CD 40 and a variable CD 86 expression on most AML cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Id primed tumor APC were heterogeneous , the majority being dendritic cells with class 2 ( + ) , CD11b ( + ) CD11c ( + ) CD 40 ( + ) CD 80 ( + ) CD 86 ( + ) markers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
All three spleen DC types would be considered as mature , based on expression of CD 80 , CD 86 , and CD 40 as well as on T cell stimulating function . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present study , expression of ( 1 ) CD 1 , a major histocompatibility complex class l like lipid antigen presenting molecule , ( 2 ) IL 10 , a cytokine promoting induction of clonal anergy , and ( 3 ) CD 80 and CD 86 , two T cell costimulators preventing induction of clonal anergy when present , was investigated in frozen sections of cervix , corpus and the fallopian tube ( FT ) of 25 day old BALB / c mice injected with FSH , progesterone or medium and of pregnant mice from each trimester ( days 7 , 14 and 19 ) . ^^^ CD 80 and CD 86 were usually absent from the female reproductive tract , but were occasionally found in the cervix during pregnancy . ^^^ This is supported by the absence of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
T cell costimulation is provided by ligation of CD 28 with either B7 . 1 ( CD 80 ) or B7 . 2 ( CD 86 ) on antigen presenting cells , and can be inhibited by a soluble form of CTLA 4 ( CTLA 4 Ig ) that binds to both B7 . 1 and B7 . 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using RT PCR amplifications with mRNA from mitogen stimulated feline peripheral blood mononuclear cells , cDNA of feline B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were cloned . ^^^ Hydrophilicity patterns of the feline CD 80 and CD 86 which were only 26 . 8 % identical at the amino acid sequence were very distinct from each other , but similar to the putative human CD 80 and CD 86 proteins , respectively . ^^^ The feline CD 80 gene encoded a protein of 292 amino acids and the CD 86 gene encoded a protein of 329 amino acids . ^^^ Although the most conserved domain among the CD 80 sequences was the Ig C like domain , the most conserved domain among the CD 86 sequences was the Ig 5 like domain . ^^^ Among the known sequences , the bovine CD 80 and the porcine CD 86 sequences available for comparisons were identified as most closely related to the feline CD 80 ( 63 . 3 % ) and CD 86 ( 67 . 5 % ) , respectively . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interleukin 12 neutralization alters lung inflammation and leukocyte expression of CD 80 , CD 86 , and major histocompatibility complex class 2 in mice infected with Histoplasma capsulatum . ^^^ The present study was designed to determine if blockade of IL 12 disrupts the protective immune response by altering the influx of lineage specific cells into infected lungs and the numbers of cells expressing CD 80 , CD 86 , CD 119 , and major histocompatibility complex class 2 ( MHC 2 ) molecules . ^^^ CD 80 ( + ) lung cells from anti IL 12 treated mice were 2 to 3 fold greater than those from controls on days 7 and 10 , whereas the total numbers of CD 86 ( + ) cells were 2 to 3 fold less and MHC 2 ( + ) cells were 1 . 5 to 2 fold less on days 3 and 5 . ^^^ Treatment with monoclonal antibodies ( MAb ) to CD 80 , CD 86 , or both reduced the fungal burden slightly compared to that in rat immunoglobulin G treated controls , whereas after IL 12 neutralization , blocking of CD 80 reduced the tissue burden by 2 . 5 fold and this correlated with a decrease in IL 4 . ^^^ Regardless , mortality was not altered by treatment with MAb to CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules was much lower on LNC than on SC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The effect of B 7 mediated costimulation on T cell homeostasis was examined in studies of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) transgenic as well as B 7 deficient mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upon in vitro culture M DC8+ cells spontaneously mature into cells with the phenotype of highly stimulatory cells as documented by the upregulation of HLA DR , CD 86 and CD 40 ; in parallel CD 80 expression is induced . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IFN gamma favours this MPhi differentiation pathway via upregulation of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These data reveal that Salmonella co incubation with BMDC as well as BMMPhi results in upregulation of MHC 1 and MHC 2 as well as several co stimulatory molecules including CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells express MHC Class 2 , CD11b , CD 14 , CD 32 , CD 68 and IL 10 , but not CD 40 , CD 80 or CD 86 , suggesting an immunosuppressive role for these dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In mammals , the classical B 7 molecules expressed on antigen presenting cells , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , bind the structurally related glycoproteins CD 28 and CTLA 4 ( CD 152 ) , generating costimulatory signals that regulate the activation state of T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Airway eosinophils , recovered after inhalational antigen challenge in sensitized mice , expressed MHC class 2 and costimulatory CD 80 and CD 86 proteins and functioned in vitro as CD 80 and CD 86 dependent , antigen specific , antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We conclude that the CD 40 ligand expressed by activated T cells first enhances MV replication in DCs , and then Fas ligand produced by activated T cells induces Fas mediated apoptosis of DCs , thus facilitating MV release . ( 2 ) Not only MV infected DCs but also bystander uninfected DCs undergo a maturation process confirmed by CD1a , CD 40 , CD 80 , CD 86 , CD 83 , and major histocompatibility complex type 2 labeling . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendritic cells were also stained for the costimulatory molecules CD 80 and CD 86 . ^^^ Fifty percent of DC expressed CD 86 and 20 % expressed CD 80 . ^^^ There was no difference in CD 80 or CD 86 expression between OB / BOS patients and stable patients . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Double staining studies using confocal laser scanning microscopy showed that the CD11c positive DCs coexpress both major histocompatibility ( MHC ) class 2 and costimulatory molecules , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD11c+ cells responded to LPS stimulation by producing high levels of interleukin 1beta ( IL 1beta ) and tumor necrosis factor alpha ( TNF alpha ) , and lower levels of IL 6 , IL 1Ra , and IL 8 and an increased expression of accessory molecules ( CD 25 , CD 40 , CD 80 , CD 86 , HLA DR , and HLA DQ ) . ^^^ PMA+I activation of CD11c+ cells resulted in high levels of IL 1beta and lower levels of IL 8 , IL 1Ra , and TNF alpha and up regulation of CD 80 , CD 86 , HLA DR , and HLA DQ . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cell surface phenotype of GM CSF DCs and IL 7 DCs was characterized by a high expression of major histocompatibility complex ( MHC ) class 1 and 2 , CD 80 , CD 86 and CD 40 . ^^^ Lipopolysaccharide ( LPS ) treatment induced the upregulation of CD 40 , CD 80 , CD 86 and the T cell stimulatory capacity in IL 4 DCs and , to a lesser extent , in the IL 7 DCs whereas GM CSF DCs responded very poorly to such treatment . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present study we determined the AF and the expression of CD11a , CD 54 , CD 58 , CD 80 , CD 86 and HLA DR costimulatory molecules by AMs lavaged from patients with pulmonary tuberculosis and controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of the costimulatory molecules CD 80 and CD 86 is differentially regulated by anti estrogens during DC differentiation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules such as CD 40 and CD 86 were slightly decreased or nonsignificantly affected ( CD 80 and MHC 2 ) . ^^^ However , after induction of DC maturation with LPS or incubation with CD 40 ligand transfected cells , D 3 DC showed marginal increases in MHC 1 , MHC 2 , CD 80 , CD 86 , CD 40 , and CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These brain DC were mature , as indicated by high level expression of MHC class 2 , CD 40 , CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have previously shown that B 7 2 ( CD 86 ) and , to a lesser extent , B 7 1 ( CD 80 ) contribute to the curative effectiveness of low dose melphalan ( L phenylalanine mustard ) for mice bearing a large MOPC 315 tumor under conditions that lead to the acquisition of potent cytotoxic T lymphocyte ( CTL ) activity at the tumor site . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This B cell effect required the presence of functional co stimulatory molecules CD 40 and B 7 2 ( CD 86 ) but not B 7 1 ( CD 80 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of major histocompatibility complex class 2 , CD 80 , CD 86 and CD11c by BMDC after phagocytosing rBCG or inert beads , was inhibited when the BMDC were pretreated with IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of molecules involved in antigen presentation and T cell activation ( HLA DR , CD 80 , CD 86 , CD 44 and CD 54 ) by cultured human osteoblasts . ^^^ BACKGROUND : Osteoblasts express the CD 44 antigen and HLA class 2 antigens , molecules which , together with other costimulatory molecules such as CD 80 , CD 86 , and CD 54 , are involved in antigen presentation and T cell activation . ^^^ Flow cytometry was used to examine the expression HLA DR , CD 80 , CD 86 , CD 44 , and CD 54 molecules involved in immune activities . ^^^ Although the cells were negative for CD 45 , the leukocyte common antigen and CD 14 ( an antigen detected on macrophages ) , they expressed CD 54 , CD 80 , and CD 86 antigens , which are also involved in the mechanisms of antigen presentation to and activation of T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Liver sinusoidal endothelial cells constitutively express all molecules necessary for antigen presentation ( CD 54 , CD 80 , CD 86 , MHC class 1 and class 2 and CD 40 ) and can function as antigen presenting cells for CD4+ and CD8+ T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cells with large , veiled cytoplasm , expressing major histocompatibility complex ( MHC ) class 2 and the costimulatory molecules CD 80 , CD 86 and CD 40 and lacking the monocyte marker CD 14 , were considered as having the phenotype of cytokine generated DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory effects of IL 2 and CpG ODN were investigated using proliferation assays , ELISA ( IL 6 , TNF alpha ) , and FACS analysis ( CD 80 , CD 86 expression ) . ^^^ In contrast , TNF alpha and IL 6 production was increased in B CLL cells , and the expression of CD 80 and CD 86 was further enhanced when IL 2 was used as a costimulus . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
No differences in the surface expression of the costimulatory molecules CD 28 , CD 152 or CD 154 was seen between immune and non immune donors after either 24 or 120 h of TSL incubation , nor were differences detected in the expression of the B 7 ligands CD 80 or CD 86 on CD14+ monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Membrane expression of costimulatory ligands CD 80 and CD 86 was similar for native peptide and p 2 , 28 40A34 , 36 and increased in activation , whereas only CD 86 was elevated during anergy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 engagement by specific monoclonal antibody ( mAb ) or binding of the natural ligands , CD 80 and CD 86 , induces tyrosine phosphorylation of CD 28 , which in turn recruits and activates the signal transducer and activator of transcription 6 ( Stat 6 ) . ^^^ The Stat 6 association with CD 28 is specifically induced by CD 80 or CD 86 ligand binding and is not dependent upon the secretion of IL 4 or IL 13 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After various intervals recovery of viable cells was measured and expression of CD 80 , CD 86 , CD 40 , CD 54 , CD 58 , and CD11a was analyzed by flow cytometry . ^^^ In cases with minor upregulation of CD 80 or CD 86 , no further stimulation using CD 40 L activation was observed . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Since the generation of a T cell response requires the expression of the costimulatory molecules CD 80 and CD 86 , we investigated their in vivo expression and their modulation in vitro during contact with responding T lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Proliferation , cytokine formation ( IL 6 , IL 8 , IL 1ra ) , and expression of surface molecules ( MHC class 1 and 2 , costimulatory molecules CD 80 and CD 86 , CD 29 , CD 54 , CD 95 ) were measured in stimulated and unstimulated cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Melanoma cells were shown to be able to co stimulate TCR triggered human T cells ; moreover , because they do not express CD 80 or CD 86 co stimulatory structures , the involvement of additional pathways have to be postulated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
For example , coadministration of costimulatory molecules ( CD 80 and CD 86 ) , proinflammatory cytokines ( interleukin 1alpha [ IL 1alpha ] , tumor necrosis factor alpha [ TNF alpha , and TNF beta ) , Th 1 cytokines ( interleukin 2 [ IL 2 ] , IL 12 , IL 15 , and IL 18 ) , Th 2 cytokines ( IL 4 , IL 5 , and IL 10 ) , and granulocytes macrophage colony stimulating factor ( GM CSF ) with DNA vaccine constructs leads to modulation of the magnitude and direction ( humoral or cellular ) of the immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To characterize the involvement of costimulatory pathways in the pathogenesis of myasthenia gravis ( MG ) , a multiparameter flow cytometry assay was adopted to enumerate blood mononuclear cells ( MNC ) expressing CD 28 , CD 80 , CD 86 , CD 40 , and CD40L molecules in patients with MG and healthy subjects . ^^^ Patients with MG had lower percentages of CD 8 ( + ) CD 28 ( + ) cells , augmented percentages of CD 4 ( + ) CD 80 ( + ) , CD 4 ( + ) CD 86 ( + ) , CD 8 ( + ) CD 80 ( + ) , CD 8 ( + ) CD 86 ( + ) , CD 14 ( + ) CD 80 ( + ) , and CD 14 ( + ) CD 86 ( + ) cells , and similar levels of cells expressing CD 40 and CD40L and of B cells expressing CD 80 and CD 86 compared to the controls . ^^^ The high percentages of CD 80 and CD 86 positive T cells and monocytes may reflect persistent activation of T and B cells , whereas the low CD 28 expression may be the result of chronic exposure to CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we show that administration of low dose melphalan ( l PAM , l phenylalanine mustard ) to mice bearing a large MOPC 315 plasmacytoma led to a rapid up regulation of B 7 1 ( CD 80 ) , but not B 7 2 ( CD 86 ) , expression on the surface of MOPC 315 tumor cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , we show that the switch to either Th 1 or Th 2 responses is not effected by differential regulation through CD 80 or CD 86 and that a Th 2 response is achieved in the presence of IL 12 . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Up regulation of monocyte and B cell co stimulatory ligands CD4O , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were also inhibited in a dose dependent manner by pdFVIII , but LFA 3 ( CD 58 ) was unchanged . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC from both pGM CSF and FL treated mice expressed high levels of surface class 2 , low levels of the costimulatory molecules CD 40 , CD 80 , and CD 86 and were equally efficient at stimulating allogeneic and Ag specific T cell proliferation in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 was shown to be functionally intact by binding to its natural ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) both in vitro and in situ . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The influence of roxithromycin ( RXM ) on the expression of co stimulatory molecules , CD 40 , CD 80 and CD 86 , was examined in vivo . ^^^ RXM treatment of the immunized mice caused suppression of CD 40 expression , but this treatment did not affect CD 80 and CD 86 expression . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypically , there was no difference between migrated LCs from RAG 1 / or SCID mice vs normal C57BL / 6 or BALB / c mice : both populations of FITC+ cells had a dendritic morphology and a mature phenotype as they expressed high levels of MHC class 2 molecules and costimulatory molecules CD 80 , CD 86 , and CD 54 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD28 / CTLA 4 interactions with their specific B 7 ligands ( CD 80 and CD 86 ) have decisive roles in antigenic and allogenic responses . ^^^ CTLA 4 was observed in the infiltrating lymphocytes in most of the biopsies , but CD 80 or CD 86 were not . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both CD 80 and CD 86 , expressed on transfected P 815 cells , were able to provide efficient costimulation for the production of IL 4 , IL 5 and IL 13 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This down regulation of the immune response was also observed when eukaryotic expression cassettes for CD 80 and for CD 86 were co administered with the pgD plasmid via the i . d . route . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that CD4+CD25+ T cells down regulated the expression of the co stimulatory molecules CD 80 and CD 86 on dendritic cells . ^^^ The steady state level of CD 80 mRNA was also decreased , while the steady state level of CD 86 mRNA was not , suggesting that distinct mechanisms regulate the expression of these molecules . ^^^ The down regulation occurred even in the presence of stimuli that would normally increase the expression of CD 80 and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This study examined the effects of blockade of the interaction between cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on the anticryptococcal DTH responses and protection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After coculture with anergic T cells expression of MHC class 2 , CD 80 and CD 86 by DC were down regulated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC infected with viable parasites up regulated the expression of CD 40 , CD 80 , CD 86 , and HLA DR and down regulated expression of CD 115 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Incubation of immature murine dendritic cells with heparan sulfate induced phenotypic maturation evidenced by up regulation of 1 A , CD 40 , CD 54 ( ICAM 1 ) , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To examine whether the Schwann cells in patients with autoimmune neuropathies have the potential to behave as professional antigen presenting cells , we investigated the expression of the co stimulatory molecules BB 1 , B 7 1 ( CD 80 ) B 7 2 ( CD 86 ) and their counter receptors CD 28 or CTLA 4 ( CD 152 ) at the protein and mRNA levels in sural nerve biopsies of patients with chronic inflammatory demyelinating polyneuropathy ( CIDP ) , CIDP associated with human immunodeficiency virus infection ( HIV CIDP ) , IgM paraproteinaemic neuropathy and normal or non immune axonal neuropathy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Both in vitro propagated normal liver derived DC , and freshly isolated bulk FL liver DC showed an immature phenotype ( MHC class 2 ( lo ) , CD 40 , CD 80 , and CD 86 ) and were weak stimulators of naive allogeneic T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Inhibiting p38SAPK prevented activation of the transcription factors ATF 2 and CREB and significantly reduced the LPS induced up regulation of CD 80 , CD 83 , and CD 86 , but did not have any significant effect on the LPS induced changes in macropinocytosis or HLA DR , CD 40 , and CD1a expression . ^^^ Inhibiting the NF kappaB pathway significantly reduced the LPS induced up regulation of HLA DR as well as CD 80 , CD 83 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On the other hand , TrkA stimulation does not change the expression of MHC , CD 80 , CD 86 , CD 40 , and CD 54 molecules , nor the antigen presenting function of monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cells displayed a characteristic iDC phenotype ( CD1a ( + ) , CD 14 ( ) , CD 80 ( + ) , CD 86 ( + ) , HLA DR ( + ) , CD 83 ( ) ) , and had potent allogeneic and antigen dependent autologous T cell stimulatory capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They provide costimulatory signals ( in particular , CD 40 and the CD 28 ligands CD 80 and CD 86 ) necessary for naive T cell activation . ^^^ Functional expression of CD 80 and CD 86 is blocked by the fusion protein cytotoxic T lymphocyte antigen 4 immunoglobulin ( CTLA4Ig ) , that promotes tolerance induction in animals . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Anti human CD 80 and CD 86 antibodies alone had minor effects in the XMLR , but in combination with hu5C8 were as effective as human CTLA 4 Ig plus hu5C8 . ^^^ Anti hCD 80 and hCD 86 antibodies that did not cross react with porcine CD 80 or CD 86 were as effective in blocking the MLR as those that did cross react , indicating that the predominant costimulation in vitro was derived from the responding cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We also investigated by FACS analysis the cell surface expression of OX 2 , CD 80 , and CD 86 on DC incubated with ODN 1 blocking MD 1 expression . ^^^ Blocking MD 1 gene expression inhibits surface expression of CD 80 and CD 86 , but not of OX 2 . ^^^ By FACS , incubation of DC with anti MD 1 Ab to promote capping eliminated cell surface expression of MD 1 and CD 14 without altering DEC 205 , DC 26 , CD 80 , CD 86 , or OX 2 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In cytofluorometric analysis the morphological changes of cells were accompanied by upregulation of the expression of the MHC class 2 , CD11c , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Oncoselective transduction of CD 80 and CD 86 in tumor cell lines using an autonomous recombinant parvovirus . ^^^ Based on their oncotropic properties , we used autonomous recombinant parvoviruses to transduce the genes coding for the constimulatory molecules CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) specifically into tumor cells without transducing normal cells . ^^^ MATERIALS AND METHODS : After infection of tumor cells by these viruses , surface expression of CD 80 and CD 86 molecules was assessed by FACS and enhancement of immunostimulatory properties was assessed in alloreactions with G 10 purified T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of CD 68 , CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , HLA DR and CD 36 may also suggest that ELCs might be related to macrophages , sinus lining or microvascular endothelial cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Maturation of DC generated by this method can be induced by treatment with double stranded RNA , LPS or TNF alpha , resulting in enhanced surface expression of CD 80 , CD 86 , CD 40 , CD 83 and MHC molecules on the DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of costimulatory molecules ( CD 40 , CD 86 , CD 80 ) at the DC cell surface correlates with their capacity to induce or suppress immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show that the CD14+CD16++ cells express dendritic cell markers : CD 40 , CD 80 , CD 86 , HLA DR , CD11b , CD11c , CD 18 , CD1a , and CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Plasma cells from patients with myeloma express a tumor specific idiotype but lack CD 80 ( B 7 1 ) and have a variable expression of CD 86 ( B 7 2 ) . ^^^ The addition of huCD40LT to short term cultures up regulated both CD 80 and CD 86 expression on B cells ( CD 19 ( + ) ) and CD 80 on plasma cells ( CD 38 ( ++ ) ) , but did not up regulate CD 86 expression on plasma cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Accordingly , flow cytometric analyses revealed a dose dependent reduction of the expression of CD 40 , CD 80 , CD 86 , 1 A , and ICAM 1 on DC with a concurrent reduction of IL 12 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
EP and DSCG suppressed the expression of costimulatory molecules , CD 40 and CD 80 , but not CD 86 , on splenic B cells which were enhanced by antigenic stimulation in vitro . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cultured cells were analyzed by flow cytometry with respect to DC associated surface molecules ( CD1a , CD 83 , CD 40 , CD 80 , CD 86 , HLA DR ) when they showed significant DC morphology in culture ( 14 cases ) . ^^^ After cultivation , neo expression or upregulation of CD1a antigen was found in 8 samples , CD 83 in 2 , CD 40 in 14 , CD 80 in 7 , and CD 86 in 9 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vitro anti tumour activity of anti CD 80 and anti CD 86 immunotoxins containing type 1 ribosome inactivating proteins . ^^^ Immunotoxins specific for the CD 80 and CD 86 antigens were prepared by linking three type 1 ribosome inactivating proteins ( RIPs ) , namely bouganin , gelonin and saporin S 6 , to the monoclonal antibodies M 24 ( anti CD 80 ) and 1G10 ( anti CD 86 ) . ^^^ The anti CD 80 immunotoxins appeared 1 2 log more toxic for target cells than the anti CD 86 ones . ^^^ The expression of the CD 80 and CD 86 molecules is prevalently restricted to antigen presenting cells and is also strong on Hodgkin and Reed Sternberg cells in Hodgkin ' s disease . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although most of the HLA DM+ cells expressed CD1a , CD 80 or CD 86 antigen , only a small proportion of CD1a+ , CD80+ or CD86+ cells expressed HLA DM . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Concurrent reductions in B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , CD 40 , MHC class 2 , CD 83 , DC lysosomal associated membrane glycoprotein ( DC LAMP ) , and CD11c expression were detected on lesional DCs , which also decreased in number within lesional biopsies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Proliferation was substantially enhanced by anti CD 80 or anti CD 152 , but was inhibited by anti CD 86 . ^^^ Furthermore , anti CD 80 partially , and anti CD 152 totally protected cloned DO . 11 . 10 T cells from the induction of unresponsiveness following culture with peptide and Chinese hamster ovary H 2 Ad+ CD 80 CD 86 cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The potential role of MRL / lpr B cells as APC , the expression of MHC class 2 molecules at their surface and their activation state ( expression of CD 69 , CD 80 / B 7 1 and CD 86 / B 7 2 molecules ) were studied . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CTLA 4 receptor ( CD 152 ) on activated T lymphocytes binds B 7 molecules ( CD 80 and CD 86 ) on APC and delivers a signal that inhibits T cell proliferation . ^^^ However , pCTLA 4 Ig bound poorly to human CD 80 and CD 86 expressed on transfectants and EBV transformed human B cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of the CD 80 and CD 86 molecules enhances cytotoxicity by human natural killer cells . ^^^ Both CD 80 and CD 86 function as costimulatory molecules in T cell cytotoxicity . ^^^ Several previous reports , most of them in the murine system , have indirectly or directly indicated the possible role of B 7 molecules ( CD 80 and CD 86 ) triggering NK cell mediated cytotoxicity in vitro . ^^^ Therefore , anti CD 80 and anti CD 86 mAbs were used in blocking experiments and both were shown to inhibit lysis by human NK cells . ^^^ The degree of inhibition observed was variable . 64 % of these NK clones were strongly inhibited by both anti CD 80 and anti CD 86 ( Type 1 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the presence of GM CSF and IL 4 , CB adherent cells became nonadherent , acquired DC morphology , and showed increased expression of CD1a , CD 80 , CD 86 , and HLA DR ; they lost membrane CD 14 and some cells with the expression of CD 83 and CMRF 44 were generated . ^^^ In the culture with TNF alpha , cells expressing CD1a , CD 80 , CD 86 , HLA DR , and HLA DQ were markedly increased . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The DC population obtained using ACBP showed higher levels of HLA class 2 molecules , co stimulatory molecules including CD 40 , CD 80 or CD 86 , and the dendritic cell marker CD 83 , compared with those generated in adult blood serum ( ABS ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this report , we show that , as a result of rAd infection , mouse bone marrow derived immature DC upregulate expression of major histocompatibility complex class 1 and 2 antigens , costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) , and the adhesion molecule CD 54 ( ICAM 1 ) . rAd transduced DC exhibited increased allostimulatory capacity and levels of interleukin 6 ( IL 6 ) , IL 12p40 , IL 15 , gamma interferon , and tumor necrosis factor alpha mRNAs , without effects on other immunoregulatory cytokine transcripts such as IL 10 or IL 12p35 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , the expression of the surface molecules CD 40 , CD 54 , CD 58 , CD 80 , CD 83 , CD 86 and the MHC class 2 molecules was upregulated after stimulation with PG . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Previous observations were extended to assess expression levels and regulatory mechanisms of costimulatory molecules CD 54 , CD 80 and CD 86 on monocytes derived from patients with early breast cancer ( EBC ) . ^^^ We report that monocytes derived from patients with EBC exhibited significantly decreased expression levels of CD 54 ( p = 0 . 0002 ) , CD 80 ( p = 0 . 009 ) and CD 86 ( p = 0 . 002 ) compared with monocytes derived from healthy females . ^^^ We conclude that monocytes derived from patients with EBC showed a simultaneous defect of expression of CD 54 and its regulation via TNF alpha , CD 80 and CD 86 as well as T cell proliferation following exposure to TT pulsed APC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Transduction with Ad IL 10 at a multiplicity of infection ( MOI ) of 50 100 resulted in a modest reduction in the incidence of DC expressing surface MHC class 2 , CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mite antigens stimulated CD 80 and the major histocompatibility complex ( MHC ) class 2 molecule expression , but suppressed CD 86 expression on B cells and spleen adherent cells that were detected by a flow cytofluorometer . ^^^ Antibodies to the MHC class 2 molecules , CD 80 and CD 86 blocked the alloantigen presenting activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Genes under the control of virus late promoters were not expressed by 10 in DC , indicating an abortive infection . 10 infection selectively reduced the surface expression of the costimulatory molecule CD 80 and the DC maturation marker CD 83 on mature DC while other surface molecules including CD 86 and MHC remained unchanged . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Astrocytes and central nervous system endothelial cells do not express B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) immunoreactivity during experimental autoimmune encephalomyelitis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using flow cytometry , we showed that CD 80 and CD 86 are expressed at increased levels on human peripheral B cells in both groups after stimulation with anti CD 40 and interleukin 4 ( IL 4 ) , but to a significantly higher extent in the AD group . ^^^ Furthermore , baseline expression of CD 80 and CD 86 on peripheral B cells was low in normal donors and increased in AD donors . ^^^ These findings suggest that interaction of CD 80 and CD 86 with their receptors CD 28 and CTLA 4 selectively promotes cell activation , including proliferation and IgE production in CD40+IL 4 stimulated peripheral blood mononuclear cells from atopic donors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On the antigen presenting cell the key players are found in the extended family of B 7 genes comprising cd 80 , cd 86 , B7h / B7RP 1 and B 7 H1 . cd 80 and cd 86 encode proteins that bind to CD 28 and CTLA 4 on T cells . ^^^ While cd 80 and cd 86 pathways act mainly on naive T cells , B7h / B7RP 1 and B 7 H1 seem to exert their effects on antigen experienced lymphocytes . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Molecular cloning of the cDNAs encoding the feline B lymphocyte activation antigen B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) homologues which interact with human CTLA 4 Ig . ^^^ We cloned the cDNAs encoding the feline homologues of B lymphocyte activation antigens B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^ We expressed recombinant feline CD 80 and CD 86 molecules by the baculovirus expression system , and demonstrated their binding ability to human CTLA 4 murine immunoglobulin fusion protein . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The resulting cells exhibited the phenotype of mature DC , as assessed by the strong expression of HLA DR , CD 80 , CD 83 and CD 86 antigens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Like conventional monocyte derived DC , designated mDC 1 , mDC 2 expressed high levels of CD11c , CD 40 , CD 80 , CD 86 , and MHC class 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Endogenous PG production by MDC does not regulate CD 40 , CD 80 , CD 86 , or HLA DR expression ; however , it does promote MDC maturation , as NS 398 significantly reduces CD 83 expression in 1 MDC matured with sCD40L / IFN gamma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , pulsing of DC with serovar Typhimurium followed by incubation for 24 or 48 h altered surface expression of MHC 1 , MHC 2 , CD 40 , CD 54 , CD 80 , and CD 86 , generating a DC population with a uniform , high expression level of these molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 40 , CD 80 , and CD 86 expression by PG containing cells was higher in RA than in OA tissues . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We evaluated the levels of expression of costimulatory molecules ( CD 80 and CD 86 ) and the production of cytokines [ interleukin ( IL ) 12 , interferon ( IFN ) gamma , IL 4 , IL 10 ] in the cord blood of mononuclear cells in high risk newborns ( n = 17 ) , and compared them with those in low risk newborns ( n = 25 ) . ^^^ Fluorescence activated cell sorter ( FACScan ) analysis was performed to determine the expressions of CD 80 and CD 86 on activated B cells and monocytes of both groups . ^^^ The expressions of CD 80 and CD 86 on B cells showed no significant difference between the high and low risk group . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
No T or B cell invasion or involvement of co stimulatory B 7 molecules ( CD 80 and CD 86 ) was observed . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
ICOS binds B7h , a molecule expressed by APC with homology to CD 80 and CD 86 . ^^^ Strikingly , ICOS up regulation is significantly reduced in the absence of CD 80 and CD 86 and can be restored by CD 28 stimulation , suggesting that CD 28 CD80 / CD86 interactions may optimize ICOS expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Female Balb / c mice were given an intraperitoneal injection of 25 microg LPS and sacrified 24 h or 72 h later to determine total cell yield , lymphocyte subpopulations ( B cells , total T cells , CD4+ and CD8+ cells ) , the costimulatory molecules CD 28 , B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) and the percentage of apoptotic cells in PP and in the spleen as well as small intestinal IgA concentration . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The FL in vitro derived DCs , when treated with interferon alpha or lipopolysaccharide during the final 24 hours of culture , expressed an activated phenotype that included up regulation of MHC class 2 , CD1d , CD8alpha , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We now extend our observations in 71 DLCL patients by analyzing malignant B cell expression of the following molecules important in T cell activation : ( a ) recognition molecules [ MHC 1 ( MAS and MCA ) and MHC 2 ( HLA DR , DP , DQ ) ] ; ( b ) CAMs [ leukocyte function antigen 1 ( CD11a and CD 18 ) and intracellular adhesion molecule 1 ( CD 54 ) ] ; and ( c ) CSMs [ B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) ] . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A significant increase in monocyte expression of CD 80 , CD 86 , CD 40 , and human leukocyte antigen DR ( HLA DR ) was also induced by live influenza virus . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The 5 2 , Ra 2 , and 6 3 clones expressed CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and MHC class 2 at low , medium , and high levels , respectively . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , thymic peptides induced the up regulation of costimulatory molecules including CD 86 , CD 80 , HLA DR and HLA ABC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show that exposure of MODC to IS PL , but not IS ODN , induced a dose dependent strong up regulation of HLA class 2 and co stimulatory molecules ( CD 80 , CD 86 ) , similar to that observed after treatment with TNF alpha . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Two populations of DC were defined : immature DC were uniformly CD1a ; mature DC expressed CD1a , CD 80 , CD 86 , HLA DR , CD 54 and CD 58 but lacked surface CD 14 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Surface expression of CD 40 , CD 80 , CD 83 , and CD 86 was increased by BCG CWS on human immature DC , and the effect was similar to those of interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , heat killed BCG , and viable BCG . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
HIV 1 infected LCs downregulated surface CD 4 and CD 83 , whereas MHC class 2 , CD 80 , and CD 86 were unchanged . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both types of neonatal EPC acquired class 2 in culture , and up regulated CD 80 and CD 86 in an adult pattern , but failed to up regulate class 2 antigen to the high level seen among cultured adult cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of CD 80 , CD 86 , human leukocyte antigen ( HLA ) DR , HLA ABC , and intracellular adhesion molecule 1 ( ICAM 1 ) was tested in human AML cell lines after the addition of HDACI , sodium butyrate ( SB ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In comparison to splenic DCs , thyroid DCs had a low CD 80 and CD 86 expression in both strains ( i . e . , an ' immature ' phenotype ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Glucocorticoids hamper the ex vivo maturation of lung dendritic cells from their low autofluorescent precursors in the human bronchoalveolar lavage : decreases in allostimulatory capacity and expression of CD 80 and CD 86 . ^^^ Here , we also show that CD 80 and CD 86 are normally expressed at low levels on CD1a+ LAF cell derived DCs compared to other DC populations . ^^^ This low level expression of costimulatory molecules is discussed here in relation to the previously reported low level expression of CD 80 ( and CD 86 ) on lung DCs in experimental animals . ^^^ DEX exposure of CD1a+ LAF cells prevented the upregulation of even this low level expression of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometry revealed an up regulation in the expression of CD 80 , CD 86 and CD 83 following activation with a soluble trimeric form of CD40L ( CD40Ls ) or LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
For this purpose , we have generated a whole series of isogenic virus stocks ( NL 4 3 backbone ) bearing or not bearing on their surface foreign CD 28 , CD 54 ( ICAM 1 ) , CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) proteins . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dex inhibited the expression of both CD 80 and CD 86 on THP 1 cells , a human acute monocytic leukemia cell line , as determined by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
There were no differences between adherent and floating DC regarding expression of CD11c , OX 62 , major histocompatibility complex class 2 , CD 80 , or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Laboratory findings included lymphocytopenia , highly elevated CD45RA / CD45R0 ratio , as well as reduced expression of the co stimulatory molecules CD 154 , CD 86 , CD 80 and CD 28 on CD4+ cells in combination with disturbed lymphocyte transformation in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In parallel , the expression by monocytes of co stimulatory molecules ( CD 40 , CD 80 , CD 86 ) , major histocompatibility complex molecules ( HLA ABC , HLA DR ) and Fcgamma receptors ( CD 16 , CD 64 ) was examined by flow cytometry . ^^^ MS patients ' blood monocytes also displayed elevated mean fluorescence intensity for the co stimulatory molecule CD 86 , and MS patients with longer disease duration ( > 10 years ) and higher disease severity ( EDSS > 3 ) had higher percentages of CD 80 expressing monocytes compared to patients with short duration or lower severity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Murine bone marrow cultured with GM CSF produced dendritic cells ( DCs ) expressing MHC class 2 ( MHC 2 ) but little CD 40 , CD 80 , or CD 86 . ^^^ Oligodeoxynucleotides ( ODN ) containing CpG motifs enhanced DC maturation , increased MHC 2 expression , and induced high levels of CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulation light : activation of CD4+ T cells with CD 80 or CD 86 rather than anti CD 28 leads to a Th 2 cytokine profile . ^^^ Another set of beads was prepared by immobilizing anti CD 3 and murine CD 80 Ig fusion protein or murine CD 86 Ig fusion protein on the beads . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
FDCP mix cells were cultured in various amounts of GM colony stimulating factor ( CSF ) and interleukin ( IL ) 4 for up to 16 d and analysed for morphology , expression of CD 34 , c kit , Gr 1 , Mac 1 , CD 40 , MHC 1 , MHC 2 and co stimulatory molecules ( CD 80 , CD 86 ) using flow cytometry , and for their capacity to present foreign antigen to autologous T cells . ^^^ The dendritic cells generated from FDCP mix cells were large , non adherent cells with veiled processes and expressed MHC 2 , CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Polymophonuclear cells ( PMN ) of healthy donors do not express major histocompatibility complex ( MHC ) class 2 antigens or the T cell costimulatory molecules CD 80 or CD 86 . ^^^ We now report that , by culturing PMN of healthy donors with autologous serum , interferon gamma ( IFN gamma ) and granulocyte macrophage colony stimulating factor ( GM CSF ) , de novo synthesis of MHC class 2 , CD 80 and CD 86 could be induced . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition to CD 80 and CD 86 , the host derived 4 1BBL is also involved in the secondary anti tumour responses . ^^^ This study indicates the complicated contribution of 4 1BBL , CD 80 and CD 86 on tumour and host cells in anti tumour immune responses and a possible therapeutic application of 4 1BBL for human tumour vaccination and gene therapy . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 154 is an activation induced CD4+ T cell surface molecule that interacts with CD 40 on antigen presenting cells ( APC ) and upregulates the key costimulatory molecules , CD 80 and CD 86 . ^^^ This review will focus on the immunobiology of CD 154 CD40 interactions and the costimulatory functions of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Extended studies demonstrated that : 1 ) ASM cells express mRNAs and proteins for the cell adhesion molecules ( CAMs ) / costimulatory molecules , CD 40 , CD40L , CD 80 , CD 86 , ICAM 1 ( CD 54 ) , and LFA 1 ( CD11a / CD18 ) ; 2 ) apart from LFA 1 , ASM cell surface expression of the latter molecules is up regulated in the presence of activated T cells ; and 3 ) pretreatment of ASM cells and tissues with mAbs directed either against CD11a or the combination of CD 40 and CD 86 completely abrogated both the activated T cell induced changes in expression of the above CAMs / costimulatory molecules in ASM cells and altered ASM tissue responsiveness . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression and function of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in an in vitro model of the human blood brain barrier . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immature DCs ( CD 80 and CD 86 deficient ) were derived from bone marrow cells propagated in GM CSF and TGF beta 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Other immunologically important molecules detected included the co stimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The importance of CD 40 , CD 80 , and CD 86 costimulatory molecules in anti Leishmania immune responses has been established in murine models . ^^^ Blockade of CD 80 , CD 86 , or both molecules ( in the order of greatest effect ) in cultures containing macrophages , PBL , and L . major significantly inhibited the production of gamma interferon , interleukin 5 ( IL 5 ) , and IL 12 . ^^^ Thus , these data suggest that CD 40 , CD 80 , and CD 86 expression and regulation may significantly impact anti Leishmania immune responses in humans . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Most cells expressed CD 80 but only a small percentage of cells were positive for CD 40 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Chronic stimulation ( 24 h ) of DCs with low , noncytotoxic ATP doses increased membrane expression of CD 54 , CD 80 , CD 86 , and CD 83 , slightly reduced the endocytic activity of DCs , and augmented their capacity to promote proliferation of allogeneic naive T lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulation with lipopeptides increased expression of CD 83 , MHC class 2 , CD 80 , CD 86 , CD 54 , and CD 58 , and decreased CD 32 expression and endocytic activity ; these lipopeptide matured DC also displayed enhanced T cell stimulatory capacity in MLR , as measured by T cell proliferation and IFN gamma secretion . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The accessory cell function of AML blasts showed no correlation with the release of any single immunomodulatory soluble mediator ( IL1beta , IL 6 , TNF alpha , soluble IL 2 receptors ) or the expression of any particular adhesion / costimulatory membrane molecule ( CD 54 , CD 58 , CD 80 , and CD 86 ) by the blasts . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mature DC from PBMC or CD 34 ( + ) cells had similar enhanced expression of MHC class 2 HLA DR , CD 80 , CD 83 , and CD 86 and were potent stimulators of mixed lymphocyte reactions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results demonstrate that quantitation of the capacity of CD 8 ( + ) CD 28 ( ) T cells from patient circulation to suppress the activation of costimulatory molecules ( CD 80 , CD 86 ) on donor APC offers a reliable tool for monitoring specific immunosuppression against the graft in solid organ transplantation . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , conditioned medium of discrete melanoma lines induced upregulation of CD 80 , CD 86 , MHC class 1 , and MHC class 2 molecules on immature DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We analyzed the phenotype of CB monocytes in the presence or absence of an inflammatory signal ( rIFN gamma ) and compared them to adult blood ( AB ) ; the expression of HLA DR and 17 different markers ( CD11a , CD11b , CD11c , CD 18 , CD 29 , CD 40 , CD 44 , CD49a , CD49d , CD49e , CD49f , CD 54 , CD 58 , CD62L , CD 80 , CD 86 and CD 102 ) was measured by flow cytometry . ^^^ Statistical analysis showed that , compared to AB , CB monocytes did not express CD11b , CD11c , CD49d and after stimulation with rIFNgamma , they lost the expression of CD 58 and CD 102 , whereas CD 80 and CD 86 expression was induced . ^^^ However , CB monocytes expressed CD 80 and CD 86 co stimulatory molecules , but this expression did not prove a normal co stimulatory function . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Thus , mast cells can phagocytize diverse particles , take up antigens , and express a number of receptors , particularly MHC class 1 and 2 antigens , ICAM 1 and 3 , CD 43 , CD 80 , CD 86 and CD40L which allow them to interact with T and B lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the M cell areas , CD 80 / CD 86 expression was much more frequent on memory ( sIgD ( ) CD 20 ( + ) ) B cells than on naive ( sIgD ( + ) CD 20 ( + ) ) B cells . ^^^ M cell areas identified by such co expression of CD 20 and CD 80 / CD 86 were always spatially related to germinal centers ( GC ) . ^^^ Contrary to the GC B cell phenotype ( sIgD ( ) CD 20 ( + ) CD 80 / 86 ( hi ) CD 10 ( + ) Bcl 2 ( ) ) , however , M cell associated B cells with a high level of CD 80 / CD 86 were CD 20 ( lo ) CD 10 ( ) Bcl 2 ( + ) , and adjacent memory T cells ( CD 3 ( + ) CD45R0 ( + ) ) often expressed CD40L ( CD 154 ) . ^^^ Autologous peripheral blood B T cell cocultures with purified protein derivative as antigen showed that the sIgD ( ) CD 80 / CD 86 ( hi ) CD 20 ( lo ) phenotype could indeed be generated during cognate B T interactions , concurrent with CD40L up regulation on memory T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
There are three identified members of this family : CD 80 , its splice variant IgV CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Paradoxically , while decreasing CD 40 and MHC class 1 expression , betaMv significantly increased the expression of MHC class 2 , CD 80 , and CD 86 on cultured LCs but impaired their allostimulatory activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This was reflected by an inhibition of CD1a , CD 40 , CD 80 , CD 86 , and HLA DR expression as well as by a reduced capacity of dimethylfumarate treated monocyte derived dendritic cells to stimulate lymphocytes in the allogeneic mixed lymphocyte reaction . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The pattern of costimulatory molecule expression ( CD 80 , CD 86 ) was primarily examined using immunohistochemistry on tissue specimens of bone / implant interface membranes taken from sites of bone erosion . ^^^ In vitro analysis also indicated phagocytosis of metal particles by differentiated U 937 caused significant induction of both CD 80 and CD 86 ( p < 0 . 01 ) , although the expression of CD 86 dominated following prolonged exposure . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
New polymorphisms of human CD 80 and CD 86 : lack of association with rheumatoid arthritis and systemic lupus erythematosus . ^^^ The crucial role of costimulatory molecules , CD 28 , CTLA 4 , CD 80 and CD 86 , for T cell activation and inhibition has been established . ^^^ In this study , we screened for polymorphisms of human CD 28 , CD 80 and CD 86 genes , and detected that polymorphisms were tested for the association with rheumatoid arthritis ( RA ) and systemic lupus erythematosus ( SLE ) . ^^^ Variations were identified in the coding regions of CD 80 ( 452G / A , 614C / G and 864A / G ) and CD 86 ( 1057A / G ) , while no variation was observed in the coding region of CD 28 . ^^^ The variations at CD 80 position 452 and CD 86 position 1057 were present in a substantial proportion of the Japanese population , and were considered to be single nucleotide polymorphisms within the coding sequence ( cSNPs ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CpG oligodeoxynucleotide ( CpG ODN ) , but not control ( non CpG ODN ) , increased the expression of costimulatory molecules ( CD 40 , CD 80 , CD 86 , CD 54 ) on malignant B cells without altering the phenotype of B cells obtained from reactive follicular hyperplasia . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They expressed insufficient amounts of MHC class 2 , CD 80 and CD 86 molecules on the surface of the cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The myeloid dendritic cells in CSF expressed higher levels of HLA DR , CD 86 , CD 80 and CD 40 than those in blood , whereas expression of these molecules by plasmacytoid dendritic cells was equal in blood and CSF . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Contemporary , no clear evidences of a role for other surface molecules modulated during DC maturation ( CD 80 , CD 83 , CD 86 and CD 40 ) , were obtained . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , AGC 10 did reduce the levels of expression of CD 86 and CD 80 , molecules known to play critical roles in B cell interactions with T lymphocytes . ^^^ Fairly large subpopulations of , but not all , B lymphocytes had their expression of CD 122 ( + ) , CD 132 ( + ) , CD 86 ( + ) and CD 80 ( + ) reduced to undetectable levels in the presence of AGC 10 . ^^^ This was denoted by comparable mean fluorescence intensity values representing the expression of CD 122 , CD 132 , CD 86 or CD 80 molecules on the surface of SACI stimulated CD 19 ( + ) cells cultured without or with AGC 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Such leukemia derived DCs expressed a sufficient level of costimulatory molecules ( CD 80 and CD 86 ) , and were shown to be monoclonal based on an the 10 inactivation analysis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we used the same model to examine the requirement for CD 28 B7 mediated T cell costimulation in the progression of established chronic rejection and examined the individual roles of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulatory molecules . ^^^ Late blockade of CD 28 B7 T cell costimulation by the fusion protein CTLA4Ig , which binds both CD 80 and CD 86 , attenuated the development of transplant arteriosclerosis , mononuclear cell infiltration , and parenchymal fibrosis in this model . ^^^ This study further defines functional differences between CD 80 and CD 86 costimulatory molecules in vivo . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
SB 203580 , an inhibitor of the p 38 MAPK , but not the extracellular signal regulated kinase l / 2 pathway blocker PD 98059 , inhibited the up regulation of CD1a , CD 40 , CD 80 , CD 86 , HLA DR , and the DC maturation marker CD 83 induced by LPS and TNF alpha . ^^^ DC maturation induced by the contact sensitizers 2 , 4 dinitrofluorobenzene and NiSO ( 4 ) , as seen by the up regulation of CD 80 , CD 86 , and CD 83 , was also coupled to the phosphorylation of p 38 MAPK , and was inhibited by SB 203580 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Following enrichment , DC developed an activated phenotype with up regulation of CD 80 , CD 86 , and CD 83 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This study assessed the value of morphological grading and the expression of the co stimulatory markers CD 40 , CD 80 and CD 86 and their ligands to predict clinical outcome in 23 consecutive low grade MALT lymphoma patients treated with H . pylori eradication . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 12 gene modified DCs ( DC / IL 12 ) displayed a more mature phenotype than nontransduced DCs , as judged by decreased expression of CD1a and increased expression of CD 83 , B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , and MHC class 1 and 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IFN beta , together with GM CSF and IL 4 , also induced maturation of IL 3Ralpha expressing cells , as reflected by upregulation of HLA DR and of the costimulatory molecules CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this review , we summarize the current understanding of these complex costimulatory pathways including the individual roles of the CD 28 , CTLA 4 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Evaluation of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) costimulatory molecules and dendritic cells on the immune response in leprosy . ^^^ In leprosy , the role of CD 80 and CD 86 as costimulatory signal in M . leprae specific cellular immunity has not yet been defined . ^^^ The cells were stimulated by M . leprae in the presence of anti CD 80 antibody ( Ab ) and / or anti CD 86 antibody ( Ab ) ( Innogenetics ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using a range of APC markers , including CD 68 , HAM 56 , HLA DR , CD1a , p 55 , RFD 1 , and costimulatory molecules CD 40 , CD 80 , and CD 86 we document the close phenotypic resemblance of rhesus and human APC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Addition of an anti IL 10 neutralizing Ab to immature DCs as well as to soluble CD 40 ligand or LPS maturing DCs led to enhanced expression of surface CD 83 , CD 80 , CD 86 , and MHC molecules and markedly augmented release of TNF alpha and IL 12 , but diminished IL 10 mRNA expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recipient mice were given donor 1x10 ( 7 ) splenocytes into the trachea with or without antibody specific for mouse CD 80 ( 1G10 ) and / or CD 86 ( GL 1 ) ( 100 microg each ) 7 days before transplantation . ^^^ CONCLUSION : Intratracheal delivery of alloantigen induced significantly prolonged survival of fully mismatched cardiac allografts and the effect was abrogated by the blockade CD 80 and / or CD 86 pathway . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : The expression of HLA DR , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) molecules was determined on CD14+ monocytes of chronic hemodialysis patients prior to a dialysis session . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After such enhanced clustering the DCs had increased their T cell stimulating capabilities in syngeneic mixed lymphocyte reaction , and had a higher expression of CD 80 and CD 86 ( signs of maturation ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting analysis showed that instant dendritomas express the key molecules for antigen presentation ( HLA A , B , C ; HLA DR ; CD 80 ; and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotype and allostimulatory function of dendritic cells treated with antisense oligodeoxyribonucleotides targeting CD 80 or CD 86 mRNA . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Administration of antisense oligodeoxyribonucleotides against mRNA of CD 80 or CD 86 prolongs survival of cardiac allografts by inhibition of CTL activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On the 7th day of culture , the cells became mature DC with a CD1a ( + ) , CD11c ( + ) , CD 80 ( + ) , CD 83 ( + ) , CD 86 ( + ) , human leukocyte antigen ( HLA ) DR ( + ) , CD 14 phenotype . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IRF 1hER stably expressing murine Hepa 1 6 HCC cells ( HepaIRF 1hER ) were characterized by lowMHC 1 , highCD 54 , and lack of MHC 2 , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CD 28 ligands CD 80 and CD 86 are expressed on APC , and both provide costimulatory function . ^^^ We have previously shown that blockade of CD 80 costimulation by Y100F Ig , a CTL associated Ag 4 ( CTLA 4 ) Ig mutant that does not bind CD 86 , inhibits the development of lung inflammatory immune responses , but does not affect blood eosinophilia or Ab production . ^^^ Each of those responses was inhibited by treatment with CTLA 4 Ig , which binds both CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These day 0 ( d 0 ) CD11c ( + ) HLA DR ( + ) lin ( ) cells comprised approximately 0 . 6 % of the mononuclear cells obtained from the lamina propria , were endocytically active , and had the phenotype of immature DC ; they were CD 40 ( + ) and expressed low levels of CD 83 and CD 86 , but little or no CD 80 or CD 25 . ^^^ The lamina propria also contained a population of cells capable of migrating out of biopsies during an overnight culture and differentiating into mature DC with lower levels of endocytic activity and high cell surface expression of CD 40 , CD 80 , CD 86 , CD 83 , and CD 25 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NOD DC , in contrast to CBA DC , have very low levels of intracellular 1 A molecules and cell surface expression of MHC class 2 , CD 80 , CD 86 and CD 40 but normal beta 2 microglobulin expression . ^^^ Incubation with the strong inflammatory stimulus of LPS and IFN gamma does not increase class 2 MHC , CD 80 or CD 86 , but upregulates the level of CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OBJECTIVES : To investigate the expression and regulation of CD 80 , CD 86 , and CD 28 costimulatory molecules in sialoadenitis and interstitial nephritis in patients with Sjgren ' s syndrome ( SS ) . ^^^ METHODS : Expression of CD 80 , CD 86 , and CD 28 molecules was studied by immunohistochemical staining of lip biopsy specimens obtained from patients who had sialoadenitis associated with SS , and renal biopsy specimens obtained from patients who had interstitial nephritis associated with SS . ^^^ To elucidate the mechanism of de novo expression of CD 80 and CD 86 antigens , their induction by cytokines in human salivary duct cell line ( HSG ) and renal cortical epithelial cells ( HRCE ) by cell enzyme linked immunosorbent assay ( ELISA ) was quantitatively investigated . ^^^ RESULTS : In patients with severe sialoadenitis , CD 80 and CD 86 were strongly expressed on ductal epithelial cells . ^^^ In patients who had interstitial nephritis associated with SS , some tubular epithelial cells expressed CD 86 but not the CD 80 antigen . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Isolated peripheral blood lymphocytes were stained for the lymphocyte subset markers CD 4 , CD 8 , CD 19 , their respective activation markers CD 25 , HLA DR , CD 38 , and the costimulatory molecules CD40L , CD 28 , CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
VLP were found to bind very well to human and mouse immune cells that expressed markers of antigen presenting cells ( APC ) such as MHC class 2 , CD 80 and CD 86 , including dendritic cells , macrophages and B cells . mAb blocking studies identified Fc gamma RIII ( CD 16 ) as one of the molecules to which the VLP can bind both on immune cells and foreskin epithelium . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The mechanism for this is that NF kappaB is essential for three aspects of antigen presenting function : blocking NF kappaB coordinately down regulates HLA class 2 , co stimulatory molecules like CD 80 , CD 86 and CD 40 , and immuno stimulatory cytokines like IL 12 and tumor necrosis factor alpha . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The surface phenotype of purified tumor APCs MB was CD11b ( + ) , CD11c ( + ) , CD 40 ( + ) , CD 80 ( + ) , CD 86 ( + ) , and MHC class 2 ( + ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The responses to lactoglobulin and PHA were inhibited by the CTLA4 / Fc chimera and by MAbs against CD 2 , CD 58 , CD 80 , and CD 86 , indicating stimulation of CD28+ LPLs with antigen presenting cells through activation of the CD 2 pathway . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Following 24 h of exposure to the fungus , DC displayed an increase in the mean fluorescence intensity of HLA DR , CD 80 , and CD 86 , and an increase in the percentage of CD 54 ( + ) cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) play important roles in the differentiation of Th 1 and Th 2 phenotypes . ^^^ CD 40 CD40L interaction supports the expression of CD 80 and CD 86 on antigen presenting cells . ^^^ We investigated the roles of CD 80 , CD 86 , and CD 40 in the differentiation of Th 1 and Th 2 subsets after stimulation with the antigen in subjects with cedar pollinosis . ^^^ After in vitro stimulation , CD 80 , CD 86 , and CD 40 expression on CD19+ cells were analyzed by flow cytometry . ^^^ These results indicate that the Th 2 response predominated in pollinosis subjects and that CD 86 rather than CD 80 may be the costimulatory molecule involved in the allergen induced activation of PBMC . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our data showed that Dex , in a dose dependent manner , down regulated surface expression of CD 86 , CD 40 , CD 54 and MHC class 2 molecules by DC , but the expression of MHC class 1 , CD 80 , CD 95 and CD95L were not affected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
An immunoperoxidase technique was used to examine CD 28 , CD 152 , CD 80 and CD 86 positive cells in gingival biopsies from 21 healthy / gingivitis and 26 periodontitis subjects . ^^^ Analysis of these cells in relation to increasing numbers of B cells in proportion to T cells and also to macrophages , suggested that CD 80 was expressed predominantly by macrophages while CD 86 was expressed by both macrophages and B cells . ^^^ Few endothelial cells expressed CD 80 or CD 86 . ^^^ Keratinocytes displayed cytoplasmic staining of CD 80 rather than CD 86 although the numbers of positive specimens in the healthy / gingivitis and periodontitis groups reduced with increasing inflammation . ^^^ In conclusion , percentages of CD 28 , CD 152 , CD 80 and CD 86 did not reflect differences in clinical status . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypically , the KG 1 derived DCs lack lineage specific markers , and express MHC class 2 , costimulatory molecules CD 80 and CD 86 , and CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The donor DCs were CD 80 ( + ) CD 86 ( + ) and , ultrastructurally , were in intimate contact with lymphoblasts within the rosettes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential role of CD 80 and CD 86 on alveolar macrophages in the presentation of allergen to T lymphocytes in asthma . ^^^ In the asthmatic lung the altered expression of costimulatory molecules ( CD 80 , CD 86 ) by alveolar macrophages contributes to T lymphocyte activation and expansion . ^^^ We hypothesized that CD 80 and CD 86 on alveolar macrophages could differentially support allergic inflammation in adult asthma . ^^^ The expression of CD 80 and CD 86 costimulatory molecules by alveolar macrophages was studied and , in parallel , the efficiency of antigen presentation was measured in terms of IL 4 and IL 5 production by allergen stimulated autologous T cells . ^^^ We found that in asthmatic subjects ( 1 ) the percent of CD80+ , but not CD86+ alveolar macrophages was increased at baseline and did not change following allergen challenge ; ( 2 ) CD 86 , but not CD 80 , membrane expression was up regulated following allergen challenge ; ( 3 ) both CD 80 and CD 86 were required to support Th 2 cytokine production by allergen specific T cells , with a prevalent role of CD 86 after allergen challenge . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , the surface expression of the costimulatory molecules CD 40 , CD 54 , CD 80 , and CD 86 was increased , indicating that CEACAM 1 induced signaling regulates early maturation and activation of dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
HOT increased the number of both CD4+ T cells and antigen presenting cells expressing MHC class 2 as well as costimulatory molecules ( CD 40 , CD 80 and / or CD 86 ) in the Peyer ' s patch ( PP ) of infant mice , which had fewer cells than adult mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Antibodies to the other co stimulatory molecules CD 40 ligand , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) , although inhibitory , were less effective . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
KM H 2 cells were demonstrated to carry the newly proposed DC associated molecules fascin , CD 83 , and DEC 205 , as well as costimulatory molecules such as CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Analysis of CD 80 and CD 86 expression showed that LC from day 3 skin expressed CD 80 , but not CD 86 and application of antigen through this skin was inefficient in upregulating CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We designed single chain Fv antibodies , monospecific and bispecific diabodies , and a bispecific tetravalent antibody ( BiTAb ) molecule directed against the CD 80 and / or CD 86 costimulatory molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
None of them expressed the immunophenotypic markers of the lymphocyte ( CD 20 , CD45RO , CD 4 , CD 8 , TCRgammadelta ) , mature monocyte ( CD 33 ) , or dendritic cell ( CD1a , CD 80 , CD 86 , S 100 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , at physiological concentrations , it profoundly inhibited CD 40 , CD 80 , CD 86 , and MHC class 2 expression on murine , GM CSF + IL 4 stimulated , bone marrow derived myeloid dendritic cells ( DC ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Infection of immature DC also led to selective and asynchronous downregulation of CD1a , CD 40 , CD 54 ( ICAM 1 ) ( 12 h postinfection ) , CD 80 ( 24 h postinfection ) , and CD 86 ( 48 h postinfection ) but not of CD11c or major histocompatibility complex class 1 and 2 molecules when compared to DC exposed to UV inactivated virus . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential incorporation of CD 45 , CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and major histocompatibility complex class 1 and 2 molecules into human immunodeficiency virus type 1 virions and microvesicles : implications for viral pathogenesis and immune regulation . ^^^ Because T cell receptor engagement of major histocompatibility complex ( MHC ) molecules in the absence of costimulation mediated via CD 28 binding to CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) can lead to anergy or apoptosis , we determined whether HIV type 1 ( HIV 1 ) virions incorporated MHC class 1 ( MHC 1 ) , MHC 2 , CD 80 , or CD 86 . ^^^ HIV infection increased MHC 2 expression on T and B cell lines , macrophages , and peripheral blood mononclear cells ( PBMC ) but did not significantly alter the expression of CD 80 or CD 86 . ^^^ HIV virions derived from all MHC 2 positive cell types incorporated high levels of MHC 2 , and both virions and microvesicles preferentially incorporated CD 86 compared to CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The surface expression of CD 14 , CD 54 , CD 64 , CD 80 , CD 86 and HLA DR was determined by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Exposure of immature DCs to CD40L transgenic cells , soluble recombinant human CD40L molecules or lipopolysaccharide induced expression of the co stimulatory molecules , CD 80 and CD 86 , and supported an allogeneic mixed leukocyte reaction . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , these cells express the accessory molecules CD 80 and CD 54 , while they lack constitutive expression of CD 86 and CD 40 , providing them the means to function as antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The use of green fluorescent protein labelled parasites allowed us to infect IL 10+ / + and IL 10 / mice and use a fluorescence activated cell sorter to distinguish infected and uninfected populations of macrophages and compare their expression of CD 80 , CD 86 and major histocompatibility complex ( MHC ) class 2 . ^^^ Taken together , these results indicate that IL 10 is not required for the virulence of the RH strain of T . gondii , nor is it involved in the regulation of the CD 80 , CD 86 and MHC class 2 molecules during RH infection . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
One approach is to genetically manipulate tumor cells to either secrete lymphokines ( GM CSF , IL 12 , IL 15 ) or express membrane bound molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IFN beta in vitro also upregulated CD 80 and , in particular , CD 86 expression on DCs . ^^^ In vitro , anti CD 80 antibody remarkably increased , while anti CD 86 antibody inhibited DC induced IL 4 production in MLR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The DC expression of human leucocyte antigen ( HLA ) molecules , CD 80 , CD 83 , and CD 86 was markedly enhanced by exposure to huCD40LT even compared to TNF alpha exposure . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This diminished T cell activation activity is due , in part , to dramatically reduced expression of the costimulation ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^ The restored T cell stimulation activity was directly correlated with induced and increased levels of CD 80 ( 16 fold ) and CD 86 ( 10 fold ) expression by the lymphokine cultured DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NK 92 also expresses other molecules implicated immune effector cell regulation ( CD 80 , CD 86 , CD40L , TRANCE ) whose relevance in NK killing is unclear . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Addition of anti CD 80 and anti CD 86 monoclonal antibody to cell cultures caused significant suppression of cell activation by antigenic stimulation . ^^^ Addition of RXM at a concentration of 5 . 0 micrograms / ml into cell cultures remarkably suppressed co stimulatory molecule , CD 40 , CD 80 and CD 86 , expressions , which enhanced by antigenic stimulation in vitro . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Surface expression of CD1d but not CD 40 , CD 80 , or CD 86 costimulator molecules was detected in hepatocytes . ^^^ Liver dendritic cells ( DC ) isolated from nontreated mice showed low surface expression of MHC , CD1d , and CD 40 , CD 80 , or CD 86 costimulator molecules that were strikingly up regulated after alphaGalCer injection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The small fraction of cells surviving in such cultures displayed a mature DC phenotype with expression of CD 83 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In normal intestinal mucosa surface expression of typical macrophage markers such as CD 14 , CD 16 , CD11b or T cell co stimulatory molecules such as CD 80 or CD 86 is low indicating anergy and low pro inflammatory activity of these cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We analyzed intracellular pathways modulating surface densities of CD 80 and CD 86 in B cells activated through ligation of the Ag receptor , and the adhesion molecule CD 54 . ^^^ Whereas B cell Ag receptor ( BCR ) cross linking alone stimulated increased expression of CD 86 , up regulation of CD 80 required dual stimulation with anti IgM and anti CD 54 . ^^^ The principal downstream component contributed by BCR signaling , toward both CD 80 and CD 86 induction , was the elevated concentration of free cytoplasmic Ca ( 2+ ) , recruited by way of capacitative influx . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The prominent ( 2 / 3 ) one is CD4+ CD45RA+ CD 33 CD11c , mostly immature as it lacks CD 80 , CD 83 and CD 86 , and is HLA DRint . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interference with CD 28 , CD 80 , CD 86 or CD 152 in collagen induced arthritis . ^^^ We have investigated interference with co stimulation by administering mAbs towards CD 28 , CD 80 , CD 86 , and CD 152 in mice immunized for the development of collagen induced arthritis ( CIA ) . ^^^ Anti CD 80 and anti CD 86 treatment inhibited disease score and incidence , whereas anti CD 28 treatment led only to a delayed disease onset . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Peritransplant macrophages and DCs from tolerant mice express down regulated CD 40 , CD 80 , and CD 86 costimulatory molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Thus , in contrast to DC pulsed with the Th 1 response inducing Ag Propionebacterium acnes , SEA exposed DC exhibit a phenotype that is most similar to that of immature DC , failing to up regulate expression of CD 40 , CD 54 , CD 80 , CD 86 , or OX40L ; producing no detectable IL 4 , IL 10 , or IL 12 ; and displaying only a minor increase in MHC class 2 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present report , we present findings on the gene sequences encoding the nonhuman primate homologues of human CD 80 , CD 86 , their ligands CD 28 and CD 152 , CD 154 , CD 95 , and CD 95 L from rhesus macaques and for phylogenetic analysis from pig tailed macaques , African sooty mangabey monkeys , baboons , and vervets as well as select molecules from the New World aotus and marmoset monkeys . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules such as CD 80 and CD 86 expressed by DCs , the polarizing cytokine environment during DC T cell interaction , and also the nature of the antigen are critical in the orientation of the immune response . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Novel transcripts encoding secreted forms of feline CD 80 and CD 86 costimulatory molecules . ^^^ Engagement of costimulatory molecules such as CD 28 or CD 152 ( CTLA 4 ) on T cells by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) dictates the nature of T cell mediated immune responses . ^^^ We previously reported the discovery of naturally occurring forms of canine CD 80 and CD 86 mRNAs which encode secreted CD 80 and CD 86 molecules . ^^^ We report here that mRNAs for secreted forms of CD 80 and CD 86 are also expressed in cats . ^^^ The detection of naturally occurring mRNAs encoding secreted CD 80 and CD 86 adds further complexity to the regulation of the B 7 CD28 / CD152 costimulatory pathway . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , expression of IL 18 and the B 7 proteins CD 80 and CD 86 are unchanged or increased compared to controls after induction of LPS tolerance . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This paralleled the induction by OMPC of CD 86 , CD 80 and CD 40 costimulatory molecules on human neonatal and murine B cells and of Th 1 cytokines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Induction therapy with monoclonal antibodies specific for CD 80 and CD 86 delays the onset of acute renal allograft rejection in non human primates . ^^^ CD 80 and CD 86 ( also known as B 7 1 and B 7 2 , respectively ) are both ligands for the T cell costimulatory receptors CD 28 and CD 152 . ^^^ Both CD 80 and CD 86 mediate T cell costimulation , and as such , have been studied for their role in promoting allograft rejection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Inhibition studies using the B 7 binding fusion protein CTLA4Ig and antibodies against CD 80 and CD 86 demonstrate that CTLA4Ig and anti CD 86 inhibited influenza specific T cell proliferation , interleukin ( IL ) 2 and interferon ( IFN ) gamma production , and generation of influenza specific CD8+ CTL . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Thus cultivated cells escaped from apoptosis , and protein synthesis was induced , notably of the major histocompatibility complex ( MHC ) class 2 antigens , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Their phenotypes ( CD 14 , CD1a , CD 83 , HLA DR , CD 80 , CD 86 and CD 40 expression ) were examined by fluorescence activated cell sorting , and their T cell stimulatory potential was investigated in allogeneic mixed lymphocyte reaction ( allo MLR ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Monocytes cultured for 7 days in 10 VIVO 15 serum free media in the presence of GM CSF + IL 4 showed down regulation of CD 14 with increased expression of HLA DR , mannose receptor , CD 80 , and CD 86 , along with highly up regulated CD1a ( + ) expression . ^^^ The addition of autologous serum to serum free media in monocyte cultures resulted in a dose dependent decrease in the CD1a ( + ) expression generating a distinct subset of CD1a ( + / ) cells expressing HLA DR , mannose receptor , CD 80 , and CD 86 . ^^^ CD1a ( + ) and CD 14 ( + ) cells expressing HLA DR , mannose receptor , CD 80 , and CD 86 were generated in 7 days from CD 34 ( + ) cells in serum free media . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Exposure of DCs during maturation to tacrolimus or CsA resulted in no significant change in the expression of DC phenotypic markers , including CD 80 , CD 86 , and HLA Class 1 and 2 antigens determined by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BACKGROUND : B 7 molecules ( CD 80 , CD 86 ) and their counter receptors , CD 28 and CD 152 ( CTLA 4 ) , play an important role in T cell mediated immune responses . ^^^ However , following the nasal provocation with house dust , not only CD 86 , but also CD 80 , CD 28 , and CD 152 were significantly expressed in allergic patients . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that Murabutide triggers immunophenotypic changes as upon treatment , iDCs up regulate the surface expression of the major histocompatibility complex type 2 molecule human leucocyte antigen DR , the co stimulatory molecules CD 80 , CD 86 and CD 40 and the differentiation marker CD 83 , and down regulate the expression of the mannose receptor . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Eosinophils from the allergic lung expressed class 2 MHC peptides , T cell costimulatory molecules ( CD 80 and CD 86 ) , and rapidly internalized and processed Ag that was sampled from within the airway lumen . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , upon contact with C . pneumoniae dendritic cells were potently stimulated because NF kappaB was activated and translocated to the nucleus , cytokines like IL 12p40 and TNF alpha were secreted , and expression of MHC class 2 molecules , CD 40 , CD 80 , and CD 86 was up regulated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The co stimulatory molecules CD 80 and CD 86 were examined by direct immunofluorescence for cell surface expression and RT PCR amplification for mRNA . ^^^ CD 80 protein was not detected at all , while an insignificant percentage of cells were positive ( mean 2 % ) for CD 86 in all cell lines tested . gamma IFN had no apparent effect on CD 80 or CD 86 protein expression . ^^^ Constitutive CD 80 or CD 86 mRNA levels were observed in 45 and 61 % of the NSCLC lines , respectively . ^^^ Overall , gamma IFN stimulation led to the up regulation of MHC Class 1 molecules and class 2 molecules as well as CD 80 and CD 86 mRNA transcripts . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The MAC DC stimulatory capacity was enhanced by LPS , although the expression of HLA class 2 , CD 83 , CD 80 and CD 86 was unmodified . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The association of molecules like CD 80 , CD 86 ( co stimulatory molecules ) in monocytes and B cells and CD 30 , CD62L , ALL , CD11a , CD 28 , CD 124 and CD 152 in CD4+ , they have shown to be of particular interest in allergic sufferings . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , we show that carcinoma cells upregulate expression of CD 54 and MHC molecules following in vitro exposure to srhCD40L but do not upregulate CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
ICOS binds to a newly identified ligand on antigen presenting cells different from the CD 152 ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Coadministration of either cyclosporine or steroids with humanized monoclonal antibodies against CD 80 and CD 86 successfully prolong allograft survival after life supporting renal transplantation in cynomolgus monkeys . ^^^ BACKGROUND : Recent studies have shown some efficacy using monotherapy with monoclonal antibodies ( mAb ) against CD 80 and CD 86 receptors after life supporting renal transplantation in non human primates . ^^^ All monkeys in groups 1 , 2 , and 4 were treated with the combination of a CD 80 ( h1F1 ) and CD 86 ( h3D1 ) mAb given at 20 mg / kg each preoperatively , then 5 mg / kg at weekly intervals starting postoperative ( po ) day 0 until poday 56 ( 9 doses ) . ^^^ In addition , combining CsA with mAbs directed against the CD 80 and CD 86 receptors significantly prolongs graft survival when compared to CsA monotherapy . ^^^ Therefore clinical trials of humanized mAbs to CD 80 and CD 86 used in combination with conventional immunosuppression can be considered . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To elucidate a role of costimulatory molecule and cell adhesion molecule in hepatic ischemia / reperfusion injury , we examined an alteration in B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and intercellular adhesion molecule 1 ( ICAM 1 ; CD 54 ) expression in the rat liver after warm ischemia / reperfusion injury . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that the synthetic PPARgamma agonist rosiglitazone ( as well as pioglitazone and troglitazone ) significantly increases on LPS and CD40L activated MDDC , the surface expression of CD 36 ( by 184 % and 104 % , respectively ) and CD 86 ( by 54 % and 48 % ) , whereas it reduces the synthesis of CD 80 ( by 42 % and 42 % ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Incubation of dendritic cells with P . c . chabaudi schizonts also resulted in up regulation of MHC class 2 , CD 40 and CD 86 but not CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In PDC , CpG ODN supported survival , activation ( CD 80 , CD 86 , CD 40 , MHC class 2 ) , chemokine production ( IL 8 , IP 10 ) and maturation ( CD 83 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Sentinel nodes ( SNs ) and non SNs were evaluated by routine pathologic analysis , and a portion of each node was processed for expression of three dendritic markers of activation ( CD 80 , CD 86 , CD 40 ) and their corresponding T cell receptors ( CTLA 4 and CD 28 ) . ^^^ Reverse transcriptase polymerase chain reaction ( RT PCR ) analyses of paired SNs and non SNs demonstrated a marked reduction in semiquantitative expression of CD 80 ( 77 % ) , CD 86 ( 77 % ) , and CD 40 ( 85 % ) , as well as CTLA 4 ( 88 % ) and CD 28 ( 85 % ) in SNs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Whereas most human DC populations uniformly express some costimulatory molecules such as B 7 2 ( CD 86 ) , previous studies have shown a wide variation in the expression of B 7 1 ( CD 80 ) among different human DC preparations . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The distribution patterns of DCs and their major costimulatory molecules , CD 80 , CD 86 and CD 40 in the spleen were identified , and the evidence for maturation of DCs in vivo in response to SEB was obtained . ^^^ This movement paralleled a maturation process , as assessed by upregulation of CD 40 , CD 80 and CD 86 expression in the interdigitating dendritic cells ( IDCs ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of the co stimulatory molecules CD 80 and CD 86 was increased and the majority of these cells retained their expression of CD 34 ( 73+ / 4 % ) and HLA DR ( 79+ / 5 % ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After human monocytes were cultured for 24 hours in serum free medium and granulocyte macrophage colony stimulating factor to produce immature DCs , additional overnight culture with either calcium ionophore ( CI ) or interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and soluble CD40L resulted in phenotypically mature DCs that produced interleukin 8 ( IL 8 ) and displayed marked expression of CD 80 , CD 86 , CD 40 , CD 54 , CD 83 , DC LAMP , and RelB . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
AF did not influence the cell surface expression of the class 2 MHC molecule and the costimulatory molecules CD 80 and CD 86 . 3 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Second , specific activation of dendritic cells was indicated by their marked up regulation in expression of costimulatory molecules B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , and ICAM 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis indicated enhanced expression of MHC class 1 and 2 molecules as well as CD 80 , CD 86 , CD 40 , and CD 54 , and reverse transcription PCR analysis revealed increased levels of interleukin 12 p 40 mRNA . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of cell surface molecules CD 40 , CD 69 , CD 80 , CD 86 , CD 95 and HLA DR was measured by flow cytometry . ^^^ MNC exposed to IFN beta in vitro enhanced expression of the co stimulatory CD 80 , CD 86 , the early activation antigen CD 69 and the cell death receptor CD 95 . ^^^ In conclusion , anti inflammatory effects of IFN beta on blood MNC include augmentation of IL 10 production and suppression of IL 12 p 40 production , which are accompanied by enhancement of CD 69 , CD 80 , CD 86 and CD 95 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The phenotype of dendritic cells was determined with antibodies to CD45RC , CD62L , and the maturation markers CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ CD62L , CD 80 , and CD 86 were lower on CD45RC ( ) OX 62 ( + ) than CD45RC ( + ) OX 62 ( + ) cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The presence of M . furfur induced maturation ( CD 83 expression ) of MDDCs , and up regulation of the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both molecules bind to the same ligands , B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) but have antagonistic functions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 is a T cell surface molecule that binds to the costimulatory molecules CD 80 and CD 86 on antigen presenting cells and downregulates T cell function . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In portal infiltrates , cells with electron microscopical and cell marker features of dendritic cells and expressing the activation markers CD 54 , CD 80 , CD 83 and CD 86 were organized in a discontinuous network , that embedded CD8+ lymphocytes in close contact with dendritic cells and came in contact with hepatocytes , sometimes infiltrating beyond the limiting plate . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Further analysis of cell surface antigens such as CD 80 , CD 86 , HLA DR , CD11a , CD 14 , CD 23 , CD 25 and CD 54 revealed that in the course of cutaneous sensitization the phenotype of the dendritic cells was not altered in the afferent lymph . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) do not have distinct roles in setting the Th1 / Th2 balance in autoimmunity in rats . ^^^ Some data suggest that the interaction between CD 28 and CD 80 ( B7 . 1 ) stimulates Th 1 responses and that CD 28 and CD 86 ( B7 . 2 ) stimulates Th 2 responses , however this is controversial . ^^^ Monoclonal antibodies ( MoAbs ) to CD 80 and CD 86 , given singly , had little effect in either model , however when given together they almost completely suppressed the HgCl 2 induced autoimmunity : the peak immunoglobulin ( Ig ) E concentration was 3 . 25 microg / ml in treated animals versus 2770 microg / ml in controls ( P < 0 . 0001 ) ; caecal vasculitis was suppressed with a median vasculitis score of 0 in treated animals versus 6 in controls ( P < 0 . 0001 ) ; and new germinal centre formation was significantly suppressed . ^^^ We have demonstrated profound suppression of both Th 1 and Th 2 driven autoimmunity in rats by a combination of anti CD 80 and CD 86 , but have been unable to demonstrate any clear differential effects . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They expressed CD 4 , CD11a , CD11b , CD11c , CD 18 , CD 32 , CD 33 , CD45R0 , CD 48 , CD 50 , CD 54 , CD 58 , CD 64 , CD 86 , CD 102 , CD 116 , CD 123 , and HLA DR ; showed no expression of CD1a and CD 83 ; and weak expression of CD 16 , CD45RA , and CD 80 . ^^^ The levels of CD 80 and CD 86 expression were comparable ; however , in contrast to G AP monocytes , CB monocytes lacked CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of the costimulatory molecules CD 80 and CD 86 on DCs from these patients ( mean , 29 % + / 17 % of MNCs and 85 % + / 10 % of MNCs , respectively ) was also normal ( mean , 29 % + / 17 % and 86 % + / 16 % of MNCs , respectively ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that MDP Lys markedly up regulated the expression of CD 80 , CD 83 , CD 86 , and CD 40 , but not human leukocyte antigen DR , and stimulated the production of tumor necrosis factor alpha , IL 6 , IL 8 , IL 10 , and IL 12 ( p 40 ) by human DCs in a dose dependent manner . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Langerhans ' cells in vernal keratoconjunctivitis express the costimulatory molecule B 7 2 ( CD 86 ) , but not B 7 1 ( CD 80 ) . ^^^ The objective of this study was to examine the expression of costimulatory molecules CD 28 , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in conjunctival biopsies from patients with active VKC and normal controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CLL DC had a similar expression of accessory molecules ( CD 54 , CD 80 and CD 86 ) as control DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The following molecules were studied : the co stimulatory molecules CD 80 , CD 86 and CD 40 ; the adhesion molecules CD11a c , CD 31 , CD 43 , CD 50 , CD 54 , CD 102 , CD 58 and CD62L ; the chemokine receptor CXCR 4 ; and the death receptors TNFR 1 and TNFR 2 and FAS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential role of CD 80 and CD 86 molecules in the induction and the effector phases of allergic rhinitis in mice . ^^^ Selective involvement of CD 80 and / or CD 86 in the differentiation of T helper ( Th ) 1 and Th 2 was seen in several diseases . ^^^ In this study , we sought to determine the differential roles of CD 80 and CD 86 in the induction and effector phase of allergic rhinitis using Schistosoma mansoni egg antigen ( SEA ) as a specific Ag . ^^^ Blockade of CD 80 at the induction phase significantly inhibited these manifestations , whereas no effect was observed by CD 86 blockade . ^^^ In contrast , the simultaneous blockade of both CD 80 and CD 86 during the effector phase partially inhibited IgE and IgG ( 1 ) production and nasal eosinophilia , although either blockade of CD 80 or CD 86 during the phase failed to inhibit these responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression pattern of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , adhesion molecules ( CD 54 ) , and intracellular cytokines ( interleukin 12p70 and tumor necrosis factor [ TNF ] alpha ) in DC after interaction with endothelial cells was determined by immunofluorescence . ^^^ Adhesion of DC to PAEC also resulted in the activation of DC , which was manifested by up regulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , adhesion molecules ( CD 54 ) , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The delayed addition of IL 4 on day 6 of culture gave rise to an additional increase in the yield of CD1a+CD14 DCs that were characterized by the expression of HLA ABC , HLA DR , CD 80 , CD 86 , and CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Distinct role of CD 80 and CD 86 in the regulation of the activation of B cell and B cell lymphoma . ^^^ To date , not much has been known regarding the role of CD 80 and CD 86 molecules in signaling of B cells . ^^^ The CD28 / CTLA4 ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , are expressed on the surface of freshly isolated splenic B cells , and their expression is up regulated by lipopolysaccharides . ^^^ In contrast , GL 1 augmented the level of anti apoptotic molecules Bcl w and Bcl 10 ( L ) and decreased the levels of pro apoptotic molecule caspase 8 , thereby providing a novel insight into the mechanism whereby triggering through CD 80 and CD 86 could deliver regulatory signals . ^^^ Thus , this study is the first demonstration of a distinct signaling event induced by CD 80 and CD 86 molecules in B cell lymphoma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interestingly , these cells have elevated levels of MHC class 2 and CD 95 , but not CD 40 , CD 80 , or CD 86 , suggesting that they are poised to undergo deletion rather than activation upon T cell encounter . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cell fraction of DC / BNL fusions showed phenotypes of DCs , MHC class 2 , CD 80 , CD 86 , and intercellular adhesion molecule ( ICAM ) 1 , which were not expressed on BNL cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In situ expression of the costimulatory molecules CD 80 and CD 86 on langerhans cells and inflammatory dendritic epidermal cells ( IDEC ) in atopic dermatitis . ^^^ Flow cytometry , skin mixed lymphocyte reactions ( SMLR ) and immunohistological analysis were performed , and showed that IDEC and not LC are the relevant cells expressing the costimulatory molecules CD 80 and CD 86 in situ . ^^^ This expression varied with the underlying diagnosis , with AD showing the highest expression of both CD 80 and CD 86 in situ . ^^^ Furthermore , the expression of CD 80 , CD 86 and CD 36 were significantly correlated . ^^^ With short term culture , both CD 80 and CD 86 were further upregulated on LC and IDEC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BACKGROUND : Constitutive expression of major histocompatibility complex ( MHC ) class 2 antigens and of the co stimulatory receptors CD 80 and CD 86 is restricted to professional antigen presenting cells . ^^^ METHODS : To continue and extend the previous study results , PMN and monocytes of 60 patients with Wegener ' s granulomatosis , 24 patients with microscopic polyangiitis ( MPA ) , 20 patients with acute bacterial infection , and 53 healthy donors were analyzed for the expression of MHC class 2 antigens as well as of CD 80 and CD 86 . ^^^ RESULTS : PMN of patients with acute , active Wegener ' s granulomatosis expressed MHC class 2 antigens , CD 80 and CD 86 ; on monocytes up regulation of MHC class 2 was seen . ^^^ In contrast , PMN of patients with inactive disease , or with relapse , patients with microscopic polyangiitis or with bacterial infections expressed neither MHC class 2 , nor CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
By genetic targeting of gp 96 onto the cell surface , we demonstrate that direct access of gp 96 to DCs induces their maturation , resulting in secretion of proinflammatory cytokines IL 1beta , IL 12 , and chemokine monocyte chemoattractant protein 1 and up regulation of the expression of MHC class 1 , MHC class 2 , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Optimal T cell activation by antigen presenting cells requires co stimulatory signaling , such as the interaction of CD 80 , CD 86 , or both with CD 28 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upon terminal differentiation with LPS or CD 40 ligand , the acquisition of a mature surface phenotype was variably achieved among WAS patients , with increased expression of CD 80 , CD 86 and DC LAMP . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After 11 days of co culture , CD 34 ( + ) derived cells display a non adherent undifferentiated morphology , a high level of monocytic CD 14 marker , a down regulated expression of LC markers ( CD1a , E cadherin ) and DC markers ( CD 40 , CD 80 , CD 54 , CD 58 , CD 83 , CD 86 , HLA DR , HLA class 1 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immunophenotypic analysis confirmed that all stimuli induced stable up regulation of CD 25 , CD 40 , CD 80 , CD 83 , CD 86 , HLA DR , DC LAMP ( CD 208 ) , and DEC 205 ( CD 205 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , signal 2 is elicited by interaction between CD 28 and its ligands ( CD 80 and CD 86 ) and is antigen independent . ^^^ Interestingly , cell surface expression of CD 80 and CD 86 on antigen presenting cell ( APC ) is regulated by stimulus via CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Comparing with CD1a DCs developed without heparin , CD1a+ DCs express higher CD 40 and CD 80 and lower CD 86 . ^^^ CD 80 , CD 86 , HLA DR , and CD 40 are proportionally up regulated when both types of DCs are stimulated with LPS or LPS plus IFN gamma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Maturation parameters such as production of IL 12 and increases in cell surface expression of HLA DR , CD 80 , CD 86 , CD 40 , and CD 83 were observed following DC treatment with MPL . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Infection induced DC morphology and altered the expression of surface markers , including loss of CD 14 , de novo induction of CD 83 and CD 25 , and strongly augmented expression of CD 86 , CD 80 , CD 40 , and HLA DR and HLA class 1 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Reduced accessory cell function was not due to changes in surface expression of monocyte costimulatory molecules ( HLA class 1 , HLA DR , CD 80 , CD 86 , CD 40 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
FACS analysis of the DC populations revealed that AAV / E6 vector pulsed DCs had higher levels of CD 80 and lower levels of CD 86 than protein pulsed DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IFN gamma and IL 4 levels in the supernatant of BLAF lymphocytes cultured with PHA were determined using sandwich ELISA and the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) expressed by AM stimulated with LPS were determined using avdin biotin complex technique . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NLCs also express CD 14 , as well as CD11b , CD 33 , CD 40 , CD45RO , CD 68 , CD 80 , CD 86 , HLA DQ , and HLA DR , but not CD1a , CD 2 , CD 3 , CD11c , CD 19 , CD45RA , CD 83 , CD 106 , or CD 154 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blocking the CD 80 and CD 86 costimulation molecules : lessons to be learned from animal models . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The effects of IPD 1151T on the expression of co stimulatory molecules , CD 40 , CD 80 and CD 86 , were investigated in vivo using mice with allergic disorders . ^^^ IPD 1151T caused suppression of CD 40 and CD 86 expression ; however , the treatments did not affect CD 80 expression . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both cell lines failed to express CD 80 , CD 86 , CD 40 , or CD 48 in vitro and in a mixed lymphocyte reaction were unable to induce T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In particular , the expression levels of HLA DR , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on DC surface from patients were also lower than that from healthy individuals ( 0 . 46 vs 0 . 92 for HLA DR , 0 . 44 vs 0 . 88 for CD 80 and 0 . 44 vs 0 . 84 for CD 86 , P < 0 . 05 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : DC cells isolated and generated were CD11c+ cells with irregular shape , and highly expressed CD 80 , CD 86 and CD 54 molecules . ^^^ H 22 cells were CD11c cells with spherical shape and bigger volume , and did not express CD 80 , CD 86 and CD 54 molecules . ^^^ H ( 22 ) DC was CD11c+ cells with bigger volume , being spherical , flat or irregular in shape , and highly expressed CD 80 , CD 86 and CD 54 molecules , too . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : CD 86 was expressed in 54 % AML , whereas CD 80 and CD1a were only occasionally positive . ^^^ Four samples enriched in CD 34 ( + ) CD 86 ( + ) AML cells differentiated into AML DC CD 86 ( + ) , CD 80 ( + ) , CD 40 ( + ) , CD11c ( + ) , HLA DR ( ++ ) , CD 14 ( + / ) that also were CD1a ( + ) or CD 83 ( + ) , after 6 days of in vitro culture with GM CSF + / TNF alpha . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A portion of each node was processed for expression of the dendritic markers of activation CD 80 , CD 86 , and CD 40 , and their corresponding T cell receptors CTLA 4 and CD 28 . ^^^ Reverse transcription polymerase chain reaction analyses of corresponding sections of the sentinel and nonsentinel nodes demonstrated a marked reduction in semiquantitative expression of CD 80 ( 77 % ) , CD 86 ( 77 % ) , and CD 40 ( 85 % ) , as well as CTLA 4 ( 88 % ) and CD 28 ( 85 % ) in sentinel as compared to nonsentinel nodes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , CD 86 expression was significantly lower on fusion positive samples ( P = 0 . 010 ) while there was no difference in expression of CD 80 or major histocompatibility complex class 1 between ETV 6 AML1 positive and negative samples . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We demonstrate that extracellular matrix ( ECM ) , hyaluronic acid ( HA ) and chondroitin sulphate A ( CSA ) , in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , can rapidly promote the differentiation of monocyte derived immature DCs , as characterized by the remarkable upregulation of human leucocyte antigen ( HLA DR ) , CD 40 , CD 54 , CD 80 and CD 86 expression to levels higher than those in the DCs generated by culturing with GM CSF and interleukin ( IL ) 4 for 7 days and aggregation of the cells within 48 h . ^^^ The upregulation of expression of HLA DR , CD 40 , CD 54 , CD 80 and CD 86 was dose dependent . ^^^ Inhibition of NF kappaB by pyrolidine dithiocarbamate and sodium salicylate , and serine threonine and tyrosine kinase by starosporine as well as phosphatidylinositide 3 kinase ( PI 3 K ) by wortmannin could prevent the effects of HA and CSA on the expression of HLA DR , CD 40 , CD 80 and CD 86 in various degrees . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 interacts with the ligands CD 80 and CD 86 on antigen presenting cells ( APC ) , and also directs the assembly of inhibitory signalling complexes that lead to quiescence or anergy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The B and T cell populations residing within the lymph follicles and / or within B cell type MALT lymphoma were characteriZed by an immunohistochemical technique , utilizing B and T cell markers including : CD 3 , CD 4 , CD 8 ( T cells ) ; CD 20 , CD 40 , GD 74 , BLA 36 , CD 80 , CD 86 ( B cells ) . ^^^ The B cell markers , CD 80 and CD 86 , were found within the centrocytic zone of the lymph follicle . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of MHC class 1 and class 2 , Intercellular cell adhesion molecule ( ICAM 1 ) , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) molecules was quantitatively analyzed by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our data show that transfection of DCs with recombinant adenovirus AdV CD40L resulted in activation of DCs with up regulated expression of proinflammatory cytokines ( IL 1beta and IL 12 ) , chemokines ( RANTES , IP 10 , and MIP 1alpha ) , and immunologically important cell surface molecules ( CD 54 , CD 80 , and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , the co stimulatory molecules CD 80 and CD 86 and the dendritic cell markers CD1a and CD 83 were scarce in SpA synovium . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
By the treatment of two step cultured cells with calcium ionophore agent ( A 23187 ) , the expression of co stimulatory molecules such as CD 86 and CD 80 ( especially CD 86 ) was up regulated . ^^^ Consistent with the up regulation of surface molecules CD 86 , CD 80 and CD 83 , the two step cultured cells treated with A 23187 also showed a stronger allo stimulatory capacity compared with the cells without A 23187 treatment . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
First , thanks to the innate immune system ' s regulation of the main costimulatory molecules CD 80 and CD 86 , the immune system rarely mistakes a pathogen for a self antigen . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A primary costimulatory signal is delivered through the CD 28 receptor after engagement of its ligands , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This was sequentially followed by the up regulation of CD 86 , CD 80 , CD 40 , and human leukocyte antigen DR expression on CD 14 ( + ) monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendritic cells were abundant in the lamina propria of pocket epithelium ; they were MHC 2 positive , admixed with CD 4 positive and CD 8 positive T lymphocytes , and , they expressed CD 54 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The capacity of this T cell subset to be directly stimulated by nickel was not due to unique antigen presenting properties , as both antigen presenting cell dependent and antigen presenting cell independent clones displayed comparable levels of HLA DR , CD 80 , and CD 86 , and were equally capable of presenting nickel to antigen presenting cell independent clones . ^^^ T T presentation induced T cell receptor downregulation , CD 25 , CD 80 , CD 86 , and HLA DR upregulation , and interferon gamma release , although to a lesser extent compared to those induced by dendritic cell T presentation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : Normal and inflamed corneas were excised from BALB / c mice , and immunofluorescence staining for CD11c , CD11b , CD 45 , CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , CD 3 , and MHC class 2 ( Ia ) was performed by confocal microscopy on wholemount corneal epithelium . ^^^ Additionally , these cells were CD 80 and CD 86 negative , reflecting an immature phenotype . ^^^ In the inflamed cornea , the expression of MHC class 2 and costimulatory molecules CD 80 and CD 86 was significantly enhanced , and present in all parts of the cornea , in contrast to the normal cornea . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These CD1a+ cells lacked the expression of CD 80 , CD 86 , or CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we compared the effect of B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on the induction of T cell immunity to C 1498 , a murine myelogenous leukemia . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Inhibition of murine corneal allograft rejection by treatment with antibodies to CD 80 and CD 86 . ^^^ The purpose of this study was to investigate the role of CD 80 and CD 86 costimulatory molecules in corneal allograft rejection . ^^^ Anti CD 80 and anti CD 86 monoclonal antibodies ( mAbs ) were administered after orthotopic corneal allograft transplantation . ^^^ Population and localization of CD 80 ( + ) and CD 86 ( + ) cells in the cornea , cervical lymph nodes , and spleen were examined by flow cytometry and immunohistochemistry . ^^^ The combined use of anti CD 80 and anti CD 86 mAbs was effective in prolonging corneal allograft survival . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules on antigen presenting cells play important roles in providing co stimulatory signals required for activation and expansion of autoreactive T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Following treatment with LPS in vivo , all DC subsets upregulated CD 40 , CD 80 , CD 86 , and MHC class 2 expression , but surprisingly Ag capture was not downregulated and some DC subsets retained expression of intracellular MHC class 2 vesicles . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypically , the expression of CD 40 , CD 80 , CD 86 and major histocompatibility complex class 2 antigen on the LCs was upregulated on the fifth and seventh day . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mature DCs expressed significantly heightened levels of their antigen presenting machinery ( e . g . , CD 54 , CD 80 , CD 86 ) and numerous cytokines and chemokines / chemokine receptors ( i . e . , Flt 3L , G CSF , IL 1alpha and 1beta , IL 6 , IL 12 , CCL 2 , 3 , 4 , 5 , 17 , and 22 , MIP 2 , and CCR 7 ) and were significantly better at inducing effector T cell responses in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cytokine production from blood cells was determined , and cell surface B 7 ( CD 80 , CD 86 ) expression was measured . ^^^ Incubation of blood cells with IL 6 resulted in increased expression of surface CD 80 and CD 86 , while IL 10 decreased CD 80 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The small number of mature B cells that do develop in Lyn deficient mice express normal levels of the transgenic BCR and lack expression of CD 80 and CD 86 , suggesting they are not activated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although the precise function of CD 83 is not known , its selective expression and upregulation together with the costimulators CD 80 and CD 86 suggests an important role of CD 83 in the induction of immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This viability was accompanied by progressive up regulation of the surface costimulatory ( CD 40 , CD 80 , CD 86 ) and activation ( CMRF 44 , CMRF 56 , CD 83 ) molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They up regulated the expression of HLA DR , CD 80 , CD 86 , partially CCR 7 but not CD 83 , partially reduced antigen uptake function , increased the levels of IL 12p35 mRNA , and prolonged surface expression of peptide MHC class 1 complexes for presentation to cytotoxic T lymphocytes , but did not induce migration towards CCL 21 chemokine . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The observed expression patterns of CD 80 and CD 86 on different APC ( B cells in type 2 and dendritic cells in type 1 ) may be responsible for this discrepancy . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vivo CD 86 blockade inhibits CD4+ T cell activation , whereas CD 80 blockade potentiates CD8+ T cell activation and CTL effector function . ^^^ To address whether a functional dichotomy exists between CD 80 and CD 86 in naive T cell activation in vivo , we administered anti CD 80 or CD 86 blocking mAb alone or in combination to mice with parent into F ( 1 ) graft vs host disease ( GVHD ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cell surface expression of the costimulatory molecules CD 80 and CD 86 was not reduced by the preincubation procedure , and only a moderate reduction of IL 6 production was observed . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We hypothesize that expression of CD 80 and / or CD 86 on porcine cells may also play a role in NK cell activation as human NK cells express a variant of CD 28 . ^^^ Our initial analysis showed that porcine endothelial cells and fibroblasts express CD 86 , but not CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Similar expression of CD1a , CD 80 , CD 86 and CD 83 were observed in both ISO DC and AD DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : Kinetics of CD 80 and CD 86 expression in the cornea and draining lymph nodes were examined by RT PCR and immunohistochemistry in untreated allograft recipients in a high responder rat model . ^^^ RESULTS : Expression of CD 80 mRNA was increased in both donor and recipient cornea 16 hours after transplantation , whereas CD 86 was detected constitutively , with no significant early increase . ^^^ Immunohistochemistry on day 5 after transplantation demonstrated major histocompatibility complex ( MHC ) class 2 expression , no CD 80 , and only a trace of CD 86 in corneal allografts . ^^^ In lymph nodes strong MHC class 2 , weak CD 80 , and moderate CD 86 expression was noted . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Accumulation of DC SIGN+CD40+ dendritic cells with reduced CD 80 and CD 86 expression in lymphoid tissue during acute HIV 1 infection . ^^^ The co stimulatory molecules CD 80 and CD 86 were , however , only partially upregulated and the complete parafollicular network found in acute EBV infection was not generated , despite increased expression of interleukins 1alpha , 1beta , 12 ; interleukin 1alpha receptor antagonist ; interferon alpha ; and CD 40 expression . ^^^ LTNP / SLP and treated aviremic subjects had increased frequency of interdigitating DC , albeit lower than in aHI , and low expression of CD 80 and CD 86 . ^^^ The infiltration of DC in LT in acute EBV infection was accompanied by upregulation of CD 80 and CD 86 expression , which did not occur in aHI . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present study , we investigated the ability of two selected APC populations , the dendritic cells ( DCs ) highly expressing CD 80 and CD 86 molecules ( CD80highCD86high ) and the monocytes expressing the same molecules at a rather low level ( CD80lowCD86low ) , to stimulate the proliferation of purified bovine WC1+gammadelta T cells to SAgs . ^^^ Finally , the addition of monoclonal antibody ( MoAb ) anti CD 86 markedly inhibited SAgs and ConA mediated proliferation , whereas MoAb anti CD 80 had no effect . ^^^ The combination of both anti CD 80 and anti CD 86 , however , suppressed this response . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These molecules include CD1a , HLA DR , CD 40 , CD 83 , CXCR 4 , and to a lower extent CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These studies also revealed that DC that matured in response to T cell mitogens display , comparatively to LPS , a distinctive phenotype characterized by high expression of the MHC class 2 , CD 40 , and CD 205 markers , but only moderate ( CD 86 ) to minimal ( CD 80 ) expression of CD28 / CTLA4 ligands . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Surface expression of CD 25 , CD 28 , CD 40 , CD 54 , CD 80 , CD 86 and CTLA 4 was evaluated on CD 3 , CD 4 , CD 8 , CD 14 and CD 19 PBMC subpopulations by three colour flow cytometry . ^^^ Levels of CD 80 and CD 86 in the control cultures were lower in the asthmatic group . ^^^ RV infection induced a significant increase of CD 80 on monocytes and of CD 86 on B cells , which occurred in both groups but were less marked in atopic asthmatic subjects . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) are functionally equivalent in the initiation and maintenance of CD4+ T cell proliferation after activation with suboptimal doses of PHA . ^^^ Many in vitro and in vivo studies demonstrated that both CD 80 and CD 86 ligands have an identical role in the activation of T cells . ^^^ Recently , functions of B 7 costimulatory molecules in vivo have been investigated in B 7 1 and / or B 7 2 knockout mice , and the authors concluded that CD 86 could be more important for initiating T cell responses , while CD 80 could be more significant for maintaining these immune responses . ^^^ In this study , we directly compared the role of CD 80 and CD 86 in initiating and maintaining proliferation of resting CD 4 ( + ) T cells in an in vitro mode system that allowed to provide the first signal to effector cells through the use of suboptimal doses of PHA and the second costimulatory signal through cells expressing CD 80 or CD 86 , but not any other costimulatory molecules . ^^^ Using this experimental system we demonstrate that the CD 80 and CD 86 molecules can substitute for each other in the initial activation of resting CD 4 ( + ) T cells and in the maintenance of their proliferative response . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This inhibition was mainly dependent upon the CD 86 pathway and to a lesser extent upon the CD 80 pathway . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Transient blocking of both B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) in addition to CD 40 CD40L interaction fully abrogates the immune response following systemic injection of adenovirus vector . ^^^ Since most previous studies have used a CTLA 4 Ig fusion molecule binding to both CD 80 and CD 86 , the respective roles of these B 7 molecules remained undefined . ^^^ We have studied the effect of blocking monoclonal Abs ( mAbs ) directed against the costimulatory molecules CD40L , CD 80 and CD 86 , alone or in different combinations , on the humoral and cellular immune responses against Ad . ^^^ Combinations of anti CD 80 + anti CD 86 or anti CD40L + anti CD 86 mAbs resulted in strong inhibition of the immune response against Ad . ^^^ However , only the blockade of both CD 80 and CD 86 in addition to CD40L fully inhibited the humoral and cellular responses against the Ad vector , such that readministration after 1 month was as efficient as in naive animals . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fluorescence activated cell sorter analysis performed at an early stage of development ( day 6 ) revealed an immature phenotype with intermediate levels of major histocompatibility complex ( MHC ) class 2 expression and low levels of the costimulator molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We find that during the acute phases of Plasmodium yoelii erythrocyte stage infection , APC upregulate the expression of class 2 MHC and CD 80 , maintain expression of CD 86 , process and present antigen , and support gamma interferon production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 expression is induced on activated B cells following stimulation by CD 86 . ^^^ We examined the effects of CD 86 ligation on surface densities of CD 80 on activated B cells . ^^^ Incubation of such CD 86 expressing B cells with anti CD 86 monoclonal antibody ( MoAb ) resulted in a marked upregulation in cell surface levels of CD 80 . ^^^ Such an enhancement in surface levels was also accompanied by a marked increase in CD 80 mRNA , suggesting that the effects of CD 86 triggering are exerted at the level of CD 80 message . ^^^ Furthermore , coculture of CD 86 upregulated B cells with CD 28 expressing fibroblasts also led to the enhancement of CD 80 levels , which was not obtained upon incubation with cells expressing cytotoxic T lymphocyte associated molecule ( CTLA ) 4 Ig . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The immune response is triggered by presentation of antigen peptides on the major histocompatibility assembly of the antigen presenting cells ( APC ) with the assistance of costimulatory molecules such as B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After infection with AdvCD40L , A 20 tumor cells up regulate several T cell costimulatory molecules ( CD 80 , CD 86 , ICAM 1 , and LFA 3 ) and Fas expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both subsets expressed TLR 7 and could respond to TLR 7 ligands , which enhanced the survival of the subsets and upregulated the surface expression of costimulatory molecules such as CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The first one is an antigen dependent one via the T cell receptor , the second one is a costimulatory signal which can be delivered by the CD 28 molecule after binding to CD 80 ( B7 . 1 ) or CD 86 ( B7 . 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells displayed a typical DC phenotype , expressing MHC class 2 , CD 54 , CD 80 , CD 86 , and CD11b / c . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The effect of inactivation of co stimulated particles B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) on T lymphocyte activity ] . ^^^ We have studied the effect of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) costimulatory molecules inactivation on IL 4 and IFN gamma production by T lymphocytes . ^^^ We have added anti CD 80 or anti CD 86 monoclonal antibodies to the monocyte / lymphocyte ( 1 : 5 ) cultures . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We also analyzed the expression of CD 80 , CD 86 , HLA DR , CD 40 , and CD 57 on the macrophage surface after stimulation with LPS in the presence or absence of T . cruzi GIPL . ^^^ Similarly , GIPL led to down modulation of CD 83 , CD 80 , CD 86 , and HLA DR surface expression and TNF alpha and IL 10 production when DC were stimulated by CD40L . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 6 inhibited the colony growth of CD 34 ( + ) DC progenitors and switched the commitment of CD 34 ( + ) cells from DCs to CD 14 ( + ) CD1a ( ) CD 86 ( ) CD 80 ( ) CD 40 ( + / ) HLA DR + / monocytic cells exerting potent phagocytic activity but no antigen presentation capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The major second signal is generated by interaction of the CD 28 molecule expressed on most T lymphocytes with its natural ligands CD 80 and CD 86 located on APCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Conversely , GM CSF treatment transformed the NKT cells into dendritic cells , inducing rapid expression of HLA DR and the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The effect of TGF beta 1 on Ag presentation was shown to be independent of the surface expression of CD 80 , CD 86 , or CD 40 costimulatory molecules by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We showed that bone marrow derived DC pulsed with Id CD40L upregulated the expression of CD 40 , CD 80 , CD 86 , and major histocompatibility complex ( MHC ) class 2 molecules with the increased production of interleukin 12 ( IL 12 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we analyzed the expression of co stimulatory molecules ( CD 80 and CD 86 ) and CD 83 , which is a marker of mature dendritic cells , on gingival cells that were isolated from severe periodontitis tissues , with the use of flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of MHC class 2 , CD 54 , CD 80 and CD 86 by prostatic dendritic cells was not up regulated after castration and freshly isolated rhFL induced prostate cells were unable to prime allogeneicT cells unless they were activated by culture either on plastic or with recombinant soluble CD 40 ligand . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) genes and genetic susceptibility to coeliac disease . ^^^ The role of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in T cell activation makes them good candidates for coeliac disease susceptibility genes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of MHC class 2 molecules , CD 40 , and CD 54 was reduced by 40 to 50 % on cells in the lytic cycle , while no decrease was observed in cell surface expression of CD 19 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both native PT ( nPT ) and genetically detoxified PT ( dPT ) efficiently promoted expression on DCs of CD 80 , CD 86 , human leukocyte antigen DR , and CD 83 markers , alloreactive antigen presentation , and cytokine production , primarily interferon ( IFN ) gamma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , stromal fibroblasts as well as monocytes provide a costimulatory signal to anti CD 3 treated T cells via CD 80 and CD 86 to maintain the enhanced activin A production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of the co stimulatory molecules CD 80 , CD 86 and CD 40 appeared similar for both DC populations and not to relate to differences in function . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 , expressed on the antigen presenting cells ( APCs ) provide costimulatory signals for T lymphocytes . ^^^ In contrast to CD 86 , expression of CD 80 on the CD14+ cells induced by GM CSF was reduced in SLE . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differentiation of monocytes under different stimulating conditions is also accompanied by the up regulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , adhesion molecules ( CD 54 ) , human leukocyte antigen ( HLA ) DR , synthesis of cytokines tumor necrosis factor alpha , IL 12p70 , IL 10 , and differential expression of message for Toll like receptors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC cultured by both methods expressed high levels of CD 86 , CD 80 , CD 40 , CD 83 , CD 44 , CD11c and CD 58 , and was comparable in their ability to induce allogeneic and peptide specific autologous proliferative responses as well as gp 100 peptide specific cytotoxic T cell responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The ratio of CD 80 : CD 86 expression was significantly lower on CD 4 ( + ) and F4 / 80 ( + ) spleen cells in CD 28 peptide treated mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
More recently , the additional expression of the costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on antigen presenting cells were found to play an important role in the activation of T lymphocytes in transplant rejection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upon ligation by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) , CD 28 induces T cell proliferation , cytokine production , and effector functions , whereas CTLA 4 signaling inhibits expansion of activated T cells and induces tolerance . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : PTL inhibited the upregulation of CD 80 , CD 83 , CD 86 , CD 40 , and MHC class 2 ; the allostimulatory function ; the production of TNF alpha and IL 12 ; and the downregulation of FITC labeled dextran uptake in human monocyte derived DCs stimulated with LPS but not with TNF alpha . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
FACS analysis of CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , and HLA DR expression on MDDC could not demonstrate significant differences in the expression of these molecules between type 1 and type 2 diabetes patients and healthy controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Surface marker expression ( HLA DR , CD 54 , CD 80 , and CD 86 ) on these DC was measured by flow cytometry after 48 h treatment with the known chemical allergens dinitrofluorobenzene ( DNFB ) and methylchloroisothiazolinone / methylisothiazolinone ( MCI / MI ) , the irritant sodium dodecyl sulfate , lipopolysaccharide ( LPS ) , and TNFalpha . ^^^ For KG 1 DC , only MCI / MI upregulated CD 86 expression , whereas TNFalpha upregulated CD 54 and slightly upregulated CD 80 and CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 2 IgG2b but not IL 15 IgG2b fusion protein reduced migration of antigen presenting cells from the skin to local lymph nodes , inhibited the expression of CD 80 and CD 86 , and induced a significant higher number of CD4+CD25+ T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In comparison with fresh DCs , the thawed DCs had similar morphology , purity , and expression of class 1 ( H 2D ( b ) and H 2K ( b ) ) and class 2 major histocompatibility complex ( MHC ) proteins , as well as CD11b , CD11c , CD 40 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , CD 80 was detected on 2 6 % of freshly isolated pro and pre B cells , and IL 7 stimulation led to induction of CD 86 on 15 20 % of pro and pre B cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Maturation of dendritic cells was induced by stimulation with native HSP 70 , was not seen with the N terminal HSP 70 ( 1 358 ) , but was enhanced with HSP 70 ( 359 610 ) , as demonstrated by up regulation of CD 83 , CCR 7 , CD 86 , CD 80 , and HLA class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment with CD 80 and CD 86 antibodies prior to administration of HgCl 2 completely suppresses the autoimmune process . ^^^ CD 80 and CD 86 antibodies , or an isotype control , were given daily for 3 days and then on alternate days until day 12 commencing either on the day of the first HgCl 2 injection ( day 0 ) or on days 4 or 8 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cross linking of MHC class 2 molecules on human monocyte derived DC results in the up regulation of the surface expression of CD 83 , CD 80 , CD 86 , CD 54 , CD1a and CD 40 molecules , the typical DC maturation associated markers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The transition from monocyte to DC is characterized by the up regulation of CD 83 , CD 86 , CD 80 , CD 40 and the down regulation of CD 14 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
With newly generated ICOS ligand ( ICOS L ) specific monoclonal antibodies we determined that human Langerhans cells in situ express similar levels of ICOS L , CD 80 , and CD 86 , compared to immature dendritic cells ( DC ) derived from monocytes in vitro . ^^^ Maturation of DC strongly up regulated CD 80 and CD 86 but did not significantly change ICOS L levels . ^^^ Our data thus indicate a selective regulation of IL 10 secretion by ICOS on re activation of effector T cells with professional antigen presenting cells ( bearing CD 80 and CD 86 ) in lymphoid tissue . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : In summary , no difference was found between both immature as well as mature DCs generated from patients and controls regarding the expression of CD1a , CD 80 , CD 86 , MHC class 1 , and MCH class 2 antigens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC ( RMAT ) displayed an up regulated expression of immune molecules ( Ia ( d ) , CD 40 , CD 54 , CD 80 and CD 86 ) and certain cytokines / chemokines , and enhanced ability of allogeneic T cell stimulation when compared to DC ( IMAT ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The absolute number and the expression of MHC 1 and 2 , CD 80 , and CD 86 both on immature and mature APCs were not significantly different in young and old mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Glomerular endothelium exhibits enhanced expression of costimulatory adhesion molecules , CD 80 and CD 86 , by warm ischemia / reperfusion injury in rats . ^^^ We have demonstrated that the hepatic sinusoidal endothelial cells have potential ability as APCs by expressing the costimulatory adhesion molecule proteins , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , of which expression was enhanced by warm ischemia / reperfusion of the rat liver . ^^^ In this study , we assessed the localization of CD 80 , CD 86 , and intercellular adhesion molecule 1 in the rat kidneys and the influence of warm ischemia / reperfusion with or without a hypercreatinemic condition on the expression of these adhesion molecules in the renal tissues . ^^^ We found that CD 80 , CD 86 , and intercellular adhesion molecule 1 proteins localized on the glomerular and peritubular endothelium and were up regulated after ischemia / reperfusion . ^^^ The relative mRNA levels analyzed by real time reverse transcription polymerase chain reaction showed that CD 80 and CD 86 expressions were constitutively observed and significantly increased for 14 days after the warm ischemia reperfusion with a peak level at Day 3 ( 6 . 7 and 20 . 8 fold increase for CD 80 and CD 86 , respectively ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To this purpose , we assessed 1 ) the expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules on peripheral , splenic , and inflammatory murine Gr 1+ PMN ; 2 ) its modulation upon interaction with C . albicans in vitro , in vivo , and in human PMN ; 3 ) the effect of Candida exposure on the ability of murine PMN to affect CD4+ Th 1 cell proliferation and cytokine production ; and 4 ) the mechanism responsible for this effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mature DC expressed significant amounts of mature DC markers ( CD 83 ) and the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Differential effects of iron deficiency on the expression of CD 80 and CD 86 co stimulatory receptors in mitogen treated and untreated murine spleen cells . ^^^ The interaction of CD 28 and its ligands ( CD 80 , CD 86 ) on antigen presenting cells and that of TCR / CD3 MHC are required for T lymphocyte activation . ^^^ To determine whether impaired lymphocyte proliferation associated with iron deficiency is due to reduced expression of these ligands , spleen cells obtained from eight to nine C57BL / 6 mice / group of iron deficient ( ID ) , iron replete ( R ) , control ( C ) , pair fed ( PF ) , and high iron ( HI ) mice were labeled with anti CD 80 fluorescein isothiocyante ( FITC ) and anti CD 86 FITC . ^^^ Lymphocyte proliferative responses to mitogens positively and significantly correlated with CD 80 and CD 86 FI ( r = 0 . 41 0 . 59 ) but negatively correlated with the percentages of CD 80 + cells ( r = 0 . 48 ) ( P < 0 . 05 ) . ^^^ Data suggest that impaired lymphocyte proliferation associated with iron deficiency is not due to reduced CD 80 and CD 86 expression . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we have re examined the specificity of CTLA 4 inhibition of MAPKs by using natural ligand with ex vivo purified CD 4 ( + ) T cells deficient in CD 80 and CD 86 ( double knockout ) , or CTLA 4 , CD 80 , and CD 86 ( triple knockout ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypic analyses revealed that Uro Vaxom activated moDCs displayed a phenotype of mature DCs with high levels of MHC molecules and increased levels of co stimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 are opposing regulators of T cell activation , triggered by the two ligands CD 80 and CD 86 . ^^^ Using CD 80 and CD 86 molecules expressed on transfected cells , we have identified a major difference between these ligands in that CD 80 transfectants have the ability to inhibit activation of resting human peripheral blood T cells via interaction with CTLA 4 , whereas CD 86 transfectants do not . ^^^ Overall , these data provide evidence that CD 80 and CD 86 differ in their interactions with CTLA 4 and that CD 80 appears to be the preferential inhibitory ligand for CTLA 4 working via a population of CD 4 ( + ) CD 25 ( + ) CTLA 4 ( + ) regulatory T cells . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Under these culture conditions we noticed upregulation or de novo expression of costimulatory molecules CD 40 , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) in 22 / 22 , 15 / 23 and 21 / 23 cases , respectively . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although new molecules continue to be discovered , the functions of B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , CD 28 , cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , inducible costimulator ( ICOS ) , programmed death 1 ( PD 1 ) , OX 40 ( CD 134 ) and CD 40 ligand ( CD40L , CD 154 ) are now sufficiently understood that immunologists are targeting them to manipulate T cells to slow the progression of autoimmune diseases or treat tumours through the increase in T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Adenoviral transduction of monocyte derived DCs ( MO DCs ) resulted in upregulation of CD 80 , CD 86 , and CD 83 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Expression of HLA DR antigens , CD 80 , intercellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule ( VCAM ) , and E selectin was immunohistochemically detected on duct cells from all patients ; however , the expression of CD 86 was limited to only some patients . ^^^ Concomitant expression of CD 80 on duct cells and Th 1 cytokine mRNA , and CD 86 on duct cells and Th 2 cytokine mRNA , was observed . ^^^ Interferon gamma ( IFN gamma ) induced the cultured salivary gland epithelial cells to express HLA class 1 antigens , HLA DR antigens , CD 80 , and ICAM 1 , while tumor necrosis factor alpha ( TNF alpha ) induced the expression of HLA class 1 antigens , CD 80 , CD 86 , and VCAM . ^^^ CD 80 and CD 86 expression of these cells was also suggested to be involved in the activation of Th 1 and Th 2 , respectively . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
HHV 6 infection of monocyte derived immature DCs appeared to induce the up regulation of CD 80 , CD 83 , CD 86 , and HLA class 1 and class 2 molecules , suggesting that HHV 6 infection induces the maturation of DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Antigen receptor triggered upregulation of CD 86 and CD 80 in human B cells : augmenting role of the CD21 / CD19 co stimulatory complex and IL 4 . ^^^ In the absence of IL 4 , BCR : CD 21 co ligation augmented BCR triggered CD 86 only under conditions of very low BCR ligand dose or affinity , and CD 80 was minimally induced by BCR and / or CD 21 crosslinking . ^^^ In the presence of IL 4 , BCR : CD 21 co ligation augmented CD 86 and CD 80 expression under conditions of greater BCR engagement . ^^^ Co ligation promoted CD 86 and CD 80 expression was associated with heightened B cell activation of resting allogeneic T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , up regulation of MHC class 2 , CD 80 and CD 86 was not altered . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here we obtained the first evidence that DC , in the absence of any foreign antigens , induce the expression of B 7 family costimulatory molecules , such as CD 80 , CD 86 , B 7 H1 , PD L 2 , B 7 H3 , and B7RP 1 , on autologous T lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OBJECTIVE : To study the modulation of the expressions of human leucocyte antigen ( HLA ) and co stimulatory molecules B 7 ( CD 80 , CD 86 ) in multidrug resistant ( MDR ) breast cancer cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression in human tubular epithelial cells in vivo and in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Allostimulatory capacity in mixed leukocyte reaction and DC marker expression ( CDla , CD 80 , CD 86 ) was not different between control and FcgammaRI cross linked DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we examined the contribution of the major costimulatory molecules , CD 40 ligand ( CD40L ) , CD 80 , and CD 86 , in the priming of CD 8 ( + ) T cells . ^^^ However , costimulation via either CD 80 or CD 86 was required , as therapeutic T cells could be generated from mice injected with either anti CD 80 or anti CD 86 Ab alone , but administration of both Abs completely inhibited the priming of therapeutic T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , other molecular components including invariant chain ( 2 ) , HLA DM , and costimulatory molecules CD 80 , CD 86 and CD 40 , are required for efficient T cell activation . ^^^ Surface expression of CD 80 , CD 86 or CD 40 was not detected although mRNA for these costimulatory molecules was present in all samples . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , when stressed apoptotic 12B1 D 1 cells were coincubated with immature dendritic cells for 24 hours , this resulted in greater up regulation of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) on the surface of dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In correlation with their selective effects on humoral and cellular immune responses , these chemokines also differentially attract CD 4 ( + ) versus CD 8 ( + ) T cells and modulate CD 40 , CD 80 , and CD 86 expressed by B 220 ( + ) cells as well as CD 28 , 4 1BB , and gp 39 expression by CD 4 ( + ) and CD 8 ( + ) T cells in a dose dependent fashion . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Transfection of the macrophagelike cell line THP 1 with the GILZ gene inhibited the expression of CD 80 and CD 86 and the production of the proinflammatory chemokines regulated on activation normal T cell expressed and secreted ( CCL 5 ) and macrophage inflammatory protein 1alpha ( CCL 3 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We also examined the influence of RXM on co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expressions on cultured splenic B lymphocytes induced by in vitro antigenic stimulation using flow cytometry . ^^^ Addition of RXM at a concentration of 5 . 0 microg / ml into cell cultures also suppressed co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expressions on splenic B lymphocytes , which was enhanced by antigenic stimulation in vitro . ^^^ Oral RXM administration for 4 weeks clearly suppressed the enhancement of CD 40 and CD 86 ( but not CD 80 ) expressions on splenic B lymphocytes induced by antigenic stimulation in vivo . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The resulting DC population exhibited typical DC morphology and expressed higher levels of MHC class 2 and the co stimulatory molecules CD 80 and CD 86 compared to the untreated peritoneal cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
No significant changes could be found in the cell surface expression of CD 80 , CD 86 , MHC 1 , MHC 2 , CD 18 , CD11b , CD 40 , CD 25 , and CD 54 in antigen presenting cells ( APC ) from control or DSG treated animals . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of surface molecules , including major histocompatibility complex ( MHC ) classes 1 and 2 , as well as CD 80 and CD 86 , increased on DCs that were stimulated with lipopolysaccaride ( DCs / LPS ) , in comparison with unstimulated bone marrow derived DCs ( BM DCs ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The fact that CD 83 is strongly upregulated together with co stimulatory molecules such as CD 80 and CD 86 during DC maturation suggests it plays an important role in the induction of immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In about 7 days , irregularly shaped floating cells with protrusions appeared and these expressed major histocompatibility complex class 2 , CD11c , CD 80 , and CD 86 , with the capacity to stimulate primary mixed lymphocyte reaction ( MLR ) and to process and present protein antigen to T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 molecule expression showed no difference in MDA MB 435 or HBL 100 cell ( P > 0 . 05 ) , and those of the other four breast cancer cells were lower than that of HBL 100 ( P < 0 . 05 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We investigated expression of costimulatory molecules BB 1 , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and their counter receptors CD 28 and CTLA 4 ( CD 152 ) in muscle biopsy specimens of patients with scleroderma polymyositis overlap syndrome ( SSc PM ) , primary polymyositis ( PM ) , and other related diseases to examine whether the muscle fibers in patients with SSc PM behave as antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Atorvastatin suppressed IFN gamma inducible expression of CD 40 , CD 80 and CD 86 co stimulatory molecules . l Mevalonate , the product of HMG CoA reductase , reversed atorvastatin ' s effects on antigen presenting cells ( APC ) and T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After phagocytosis , cells were stained for MHC class 2 molecules , CD 14 , CD 80 , and CD 86 to identify the phagocytic population . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These mature DCs could be MHC class 2 negative , but had to bear CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Role of CD 80 , CD 86 , and CTLA 4 on mouse CD 4 ( + ) T lymphocytes in enhancing cell cycle progression and survival after activation with PMA and ionomycin . ^^^ Cell surface interactions between the T cell costimulatory receptors , CD 28 and cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , with their cognate ligands , CD 80 and CD 86 , on antigen presenting cells play an important role in T cell activation . ^^^ Although CD 80 and CD 86 are induced on T cells after activation , not much is known about their role in modulating T cell function . ^^^ We show that CD 80 , CD 86 , and CTLA 4 are induced on purified CD 4 ( + ) T cells after in vitro activation with phorbol 12 myristate 13 acetate ( PMA ) and ionomycin , and they play an essential role for proliferation and survival . ^^^ This study reveals a functional role for CD 80 , CD 86 , and CTLA 4 on CD 4 ( + ) T lymphocytes and sheds light on the mechanisms by which these molecules enhance activation and survival with PMA and ionomycin . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , the required CD154 / CD40 interaction is shown to be independent of CD 80 and CD 86 expression : CD80 / CD86 ( / ) B 6 mice develop MAIDS after LP BM 5 infection . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells were CD 10 , CD 44 , CD 54 , CD 80 , CD 86 and HLA DR positive , as previously described . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In general , DC phenotype was CD 14 ( low ) , CD 86 ( high ) , CD 40 ( high ) , CD 80 ( low ) , and CD 83 ( low ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Chronic stimulation with adenosine enhanced the macropinocytotic activity and the membrane expression of CD 80 , CD 86 , major histocompatibility complex ( MHC ) class 1 , and HLA DR molecules on iDCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : We found that : 1 ) mDC generated in the presence of GST showed lower expression of CD1a , CD 83 , CD 80 , CD 86 , HLA ABC and HLA DR compared to control mDC on FACS analysis . 2 ) GST treated mDC showed reduced capacity of stimulating allogenic T cells in mixed leukocyte reaction . 3 ) IL 12p70 production after stimulation with SAC or LPS plus IFN gamma was markedly reduced in GST treated mDC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cells phenotypes were identified using three color flow cytometry analysis for the following receptors HLA DR , CD 80 , CD 83 , CD 86 , CD11c , CD 18 , CD 54 , CD 58 , CD 3 , CD 4 , CD 8 , CD 19 , CD 20 , CD 14 , CD 16 , CD 56 . ^^^ HLA DR molecules , co stimulatory receptors CD 80 , CD 86 , CD 83 and adhesion molecules CD 18 , CD11c , CD 54 , CD 58 , were analyzed by two color immunofluorescence microscopy on ST serial sections . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They expressed very low levels of costimulatory molecules ( intercellular adhesion molecule [ ICAM ] 1 , CD 40 , CD 80 , CD 86 ) and of the T cell area homing chemokine receptor CCR 7 , whereas they showed higher levels of CCR 2 and CCR 6 , as revealed by reverse transcription PCR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The contribution of IL 16 to the activity was evaluated through use of a neutralizing monoclonal antibody ; the contribution of CD28 / B7 costimulation to allergen induced release of TLCA was determined through use of CTLA 4 Ig fusion protein and neutralizing monoclonal antibodies to CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) . ^^^ Antibodies to the CD 28 counter ligands CD 80 and CD 86 inhibited allergen induced release of TLCA in mild asthmatic explants by 94 % ( P < . 05 ) and 62 % , but TLCA release from moderate asthmatic explants was unaffected by CTLA 4 Ig . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We investigated if B 7 H1 would be up regulated in HIV infection , a disease characterized by increased IL 10 production , by measuring B 7 H1 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) expression and mRNA in 36 HIV infected patients and in 22 healthy controls ( HCs ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Combination induction therapy with monoclonal antibodies specific for CD 80 , CD 86 , and CD 154 in nonhuman primate renal transplantation . ^^^ BACKGROUND : Antibodies and fusion proteins specific for CD 80 , CD 86 , and CD 154 have shown promise as agents capable of inducing donor specific tolerance in rodents . ^^^ In the nonhuman primate , monoclonal antibodies specific for CD 80 and CD 86 have prolonged the time to rejection of renal allografts but have not resulted in tolerance . ^^^ METHODS : A combination of monoclonal antibodies specific for CD 80 , CD 86 , and CD 154 were used in a mismatched nonhuman primate renal allograft model . ^^^ RESULTS : Treatment of four rhesus macaques with this combination yielded a mean rejection free survival of 565 days ( 311 911 days ) , significantly greater than untreated controls ( mean survival=7 . 0 days , P=0 . 001 ) and animals treated with only a combination of anti CD 80 and CD 86 ( mean survival=191 days , P=0 . 01 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upon cultivation with interferon gamma ( IFN gamma ) and granulocyte / macrophage colony stimulating factor ( GM CSF ) polymorphonuclear neutrophils ( PMN ) acquire characteristics of dendritic cells , including expression of major histocompatibility complex ( MHC ) class 2 antigens , of the co stimulatory antigens CD 80 , CD 86 and of CD 83 , the latter considered to be specific for dendritic cells . ^^^ During acute infection up to 80 % of PMN acquired CD 83 , but remained negative for MHC class 2 , CD 80 or CD 86 . ^^^ PMN of patients with primary vasculitis expressed MHC class 2 antigens , CD 80 and CD 86 , but not CD 83 , indicating that up regulation of MHC class 2 and of CD 83 are not necessarily linked to each other . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , after exposure to BCG infected necrotic macrophages , DCs underwent phenotypic changes , including the up regulation of maturation specific markers ( major histocompatibility complex class 2 , CD 40 , CD 80 , and CD 86 ) and the capacity to stimulate antigen specific CD4+ T cells with higher efficiency than when they were directly infected with a similar number of bacteria . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Forty to fifty percent of both samples , frozen / thawed as well as fresh BMDC , exhibited characteristic DC morphology , and the DC obtained from the frozen / thawed samples expressed a similar level of MHC class 1 , MHC class 2 , CD 80 , CD 86 , CD11c , CD11b , CD 54 and CD 205 molecule as fresh DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulation of CD11c ( + ) B 220 ( + ) pDCs with oligodeoxynucleotides containing certain CpG motifs plus CD 40 ligand plus GM CSF led to increased MHC class 2 , CD 80 , CD 86 , and CD8alpha expression levels , to a switch in chemokine receptor expression that affected their migration , to IFN alpha and IL 12 secretion , and to the acquisition of priming capacities for both CD 4 ( + ) and CD 8 ( + ) OVA specific TCR transgenic naive T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These included T cell costimulatory molecules CD 80 , CD 86 , CD 40 , and CD 54 and high levels of surface MHC class 1 and 2 on CD11c ( + ) cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC maturation was monitored by flow cytometric analyses for the expression of surface markers ( HLA DR , CD1a , CD 40 , CD 80 , CD 86 , CD 83 , CD 14 , CD 32 , mannose receptor , and CD11c ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
High expression of CD 28 , CD 80 , CD 86 , CD 40 , CTLA 4 , CD44v variants , and FasL occurred in alopecia areata resistant mouse spleens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 80 , CD 83 , CD 86 , and major histocompatibility complex class 1 and class 2 antigens were reduced on lipopolysaccharide ( LPS ) matured leukaemic DC but were enhanced by a mixture of interleukin 1beta ( IL 1beta ) , IL 6 , tumour necrosis factor alpha ( TNF alpha ) and prostaglandin E 2 ( PGE 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 4 T cell activation is positively ( CD 28 ) and negatively ( CTLA 4 ) regulated by the costimulatory ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CD 86 ( + ) major histocompatibility complex class 2 ( + ) ( MHC 2 ) ( + ) cells and CD 28 ( + ) CD 4 ( + ) T cells were decreased by SST treatment , while CD 80 ( + ) MHC 2 ( + ) cells , CD 40 ( + ) MHC 2 ( + ) cells and CD 154 ( + ) CD 4 ( + ) T cells showed no change . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD11c+ cells from ProGP 4 treated mice co expressed DEC 205 and also expressed CD 80 , CD 86 and CD 40 , albeit at lower levels as compared to Class II+ / CD11c+ cells from untreated animals . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To determine whether this effect might involve modulation of the expression of accessory molecules known to be important in T cell activation , we analyzed by flow cytometry the influence of vIL 10 on the basal as well as on IFN gamma induced up regulation of HLA molecules , ICAM 1 , and two members of the B 7 family B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) at the surface of human monocytes . ^^^ Viral IL 10 in a concentration dependent manner inhibited both basal and IFN gamma induced HLA class 2 , ICAM 1 ( basal levels of ICAM 2 and ICAM 3 is unaffected ) , CD 80 , and CD 86 up regulation when added simultaneously with IFN gamma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendritic cells ( DCs ) not only induce but also modulate T cell activation . 1 , 25 dihydroxyvitamin D ( 3 ) [ 1 , 25 ( OH ) ( 2 ) D ( 3 ) ] induces DCs with a tolerogenic phenotype , characterized by decreased expression of CD 40 , CD 80 , and CD 86 costimulatory molecules , low IL 12 and enhanced IL 10 secretion . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
To observe the proliferation of T lymphocytes stimulated by CML and AML cells which were induced by rhGM CSF and rhIL 4 , and the secretion of IFN gamma from proliferated T lymphocytes , the expression of CD 80 , CD 86 and HLA DR on CML and AML cells induced by GM CSF and IL 4 was assayed by flow cytometry in vitro . ^^^ The results showed that GM CSF and IL 4 significantly upregulated the expression of CD 80 , CD 86 and HLA DR on CML cells and CD 80 and CD 86 on AML cells , which could stimulate the T lymphocyte proliferation and high secretion of IFN gamma ( in CML group ) of autologous T lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The phenotype of DC was detected by FCM with CD1a , CD 40 , CD 80 , CD 86 , HLA A , B , C and HLA DR monoclonal antibodies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Therefore , the authors analyzed the expression kinetics of MHC class 2 ( 1 Ak ) / HEL peptide `` complex , ' ' total MHC class 2 , CD 80 , and CD 86 on in vitro or in vivo CD 40 stimulated DCs over a period of 5 days . ^^^ When purified DCs were co cultured in vitro with antigen specific T cell hybridomas , the DCs had lower expression of total MHC class 2 and `` complex , ' ' but did not reduce their CD 80 and CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD8alpha ( + ) DC from schistosome infected mice exhibited a 2 to 3 fold increase in the expression of MHC class 2 , CD 80 , and CD 40 ( but not CD 86 ) compared with DC from uninfected control animals , while CD8alpha ( ) DC exhibited a 2 to 3 fold increase in the expression of MHC class 2 and CD 80 and no alteration , compared with DC from uninfected mice , in the expression of CD 86 or CD 40 . ^^^ In contrast , infection with T . gondii resulted in a more pronounced increase in the expression of activation associated molecules ( MHC class 2 , CD 80 , CD 86 , and CD 40 ) on both CD8alpha ( ) and CD8alpha ( + ) splenic DC and promoted elevated IL 12 production by DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC infected with S . typhimurium up regulated cell surface expression of major histocompatibility class 1 ( MHC 1 ) , MHC 2 , CD 40 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Preadipocytes rapidly and massively acquired high phagocytic activity and index . 60 70 % of preadipocytes also expressed five macrophage specific antigens : F4 / 80 , Mac 1 , CD 80 , CD 86 , and CD 45 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Large cells , differentiated after seven days of culture , expressed high levels of MHC 2 molecules and Mannose Receptor , variable levels of CD 80 and CD 86 , and low levels of CD1a . ^^^ CD40L matured DC from JMML was associated with relevant increase of CD 80 , CD 86 and CD 83 , increased APC activity , responded in chemotaxis assays to MIP 3beta and secreted increased amounts of macrophage derived chemokine ( MDC ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , with Der p 1 pulsed P DC from both groups , no increase in human leukocyte antigen DR , CD 80 , and CD 86 and no IL 10 secretion were detected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cultured DCs were shown to express class 1 , class 2 , CD 80 and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The most mature APC were generated by calcium ionophore A 23187 plus IL 4 , as evidenced by the higher expression of CD 40 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC differentiated in the presence of rapamycin ( rapa DC ) had increased expression of CD1a , CD1b , and CD1c and decreased expression of MHC 1 , MHC 2 , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upon stimulation by infectious agent products , dendritic cells ( DC ) become activated , express high levels of class 1 and class 2 antigens , CD 80 , CD 86 and CD 83 and migrate to secondary lymphoid organs where they can prime naive CD 4 helper and CD 8 cytotoxic T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Uptake of antigen by DC and the degree of expression of the cell surface markers MHC class 2 , CD 80 , CD 86 and the DC maturation marker CD 83 , was investigated by flow cytometry following incubation with liposomes or solution containing FITC conjugated antigen . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upon exposure to either extracellular or intracellular bacteria , DC underwent maturation as assessed by the increased levels of expression of CD 80 , CD 86 , and HLA molecules and the de novo expression of CD 83 and CCR 7 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Like the positive control TNF alpha , the mistletoe extract significantly activated CD 80 and CD 86 as well as HLA class 1 and 2 molecules on these cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that , unlike H2O2 , reactive oxygen species generated by the reaction of oxidase on xanthine induced early phenotypic maturation of dendritic cells by upregulating specific markers CD 80 , CD 83 , and CD 86 and downregulating mannose receptor mediated endocytosis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : MHC class 2 ( + ) CD 80 ( + ) CD 86 ( + ) cells were found in the periphery of the anterior normal stroma . ^^^ In a surprising finding , significant numbers of MHC class 2 ( ) CD 80 ( ) CD 86 ( ) cells were found in the center of the anterior stroma . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 40 ligation had no effect on cell viability or surface expression of CD 54 , CD 80 , CD 86 or CD 95 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both Ad Neo and Ad IL 4 transduced DC displayed upregulated surface MHC class 2 and costimulatory molecules ( CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC infected with any of the Ad vectors expressed higher levels of CD 40 , CD 80 , and CD 86 molecules than uninfected DC and Ad . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After being stimulated with PTX , DC exhibited up regulation of MHC class 2 , CD 80 , CD 86 , CD 40 , and DEC 205 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 C domains play an important role in receptor binding and co stimulatory properties . ^^^ CD 80 and CD 86 expressed on the surface of antigen presenting cells interact with cytotoxic T lymphocyte antigen 4 [ CTLA 4 ( CD 152 ) ] expressed on activated T cells and mediate critical T cell inhibitory signals . ^^^ CD 80 and CD 86 are type 1 glycoproteins , and are made up of two extracellular ( EC ) Ig like domains a transmembrane region and a cytoplasmic tail . ^^^ Co crystallographic structures of both CD 80 and CD 86 bound to CTLA 4 indicate that there is no direct interaction of the C domain of either CD 80 or CD 86 with the CTLA 4 . ^^^ To further understand the importance of C domains in the functioning of CD 80 and CD 86 , we constructed chimeric human CD 80 and CD 86 molecules by swapping their respective C domains , and tested their ability to stimulate T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Many solid tumours have been shown to lack expression of either of the immune co stimulatory molecules CD 80 ( B7 . 1 ) or CD 86 ( B7 . 2 ) , which is thought to be one of the ways in which tumours may escape immune recognition . ^^^ We have examined the surface expression of CD 80 , CD 86 , human leucocyte antigen ( HLA ) class 1 and 2 , CD11a , CD 54 , and CD 58 on the blast cells from patients with acute myeloid leukaemia ( AML ) at presentation . ^^^ There was no significant difference in duration of first remission with expression of CD 80 , CD 86 , CD11a , CD 54 or HLA class 2 . ^^^ However , when expression of CD 80 and CD 86 were considered together , a significantly longer duration of remission was found . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : The T DCs expressed specific surface markers ( CD 83 , CD1a , CD 54 , CD11a , CD 80 , CD 86 , HLA DR , CD83HLA DR ) of mature DCs , and stimulated strong T cell proliferative responses in both autologous and autogeneic MLR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
MSC express major histocompatibility complex ( MHC ) class 1 and lymphocyte function associated antigen ( LFA ) 3 antigens constitutively and MHC class 2 and intercellular adhesion molecule ( ICAM ) 1 antigens upon gamma interferon treatment but do not express CD 80 , CD 86 , or CD 40 costimulatory molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Such modifications include a reversal of CD4 / CD8 ratio , activated phenotype ( increased expression of CD45RO , CD 95 , and HLA DR ) , and acquisition or increased expression of co stimulatory molecules ( CD 80 , CD 86 , and CD 40 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , cellular activation markers ( CD 69 , CD 25 , HLA DR ) on T lymphocytes and costimulatory molecules ( CD 80 , CD 86 ) on macrophages were studied . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment of microglia with supernatants from allo antigen and myelin basic protein specific Th 1 cell lines augmented expression of cell surface molecules MHC class 2 , CD 80 , CD 86 , CD 40 , and CD 54 , enhanced the functional antigen presenting cell capacity of microglia in a mixed lymphocyte reaction , and increased cytokine / chemokine secretion ( TNFalpha , IL 6 , and CXCL10 / IP 10 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
GM CSF also stimulated differentiation and maturation of TDC as judged by higher expression of MHC class 1 and 2 molecules , CD 54 , CD 80 and CD 86 . ^^^ The influence of unstimulated TDC on proliferation of thymocytes was inhibited by anti CD 86 but not anti CD 80 mAb , whereas in cultures with GM CSF treated TDC both mAbs exerted an additive blocking effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
GM CSF recruited DCs were CD11c ( + ) DEC 205 ( ) and had high expression of major histocompatibility complex ( MHC ) class 2 , CD 54 , and CD 80 but low CD 40 and CD 86 staining . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In support of this hypothesis , recent studies have shown that neutrophils , when activated by the correct combination of cytokines , can be induced to express cell surface major histocompatibility complex ( MHC ) Class 2 ( DR ) antigen , CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) : molecules required for antigen presentation and subsequent T cell activation . ^^^ Cytoplasmic MHC Class 2 antigen was found with two out of 20 normal donors tested whereas cytoplasmic CD 80 and CD 86 were found to a variable extent within all normal donors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , hCD 152 hCD59 or pCD 152 hCD59 expression resulted in a significant reduction in T cell activation as the result of CD 152 engagement of porcine CD 86 or murine CD 80 in when Jurkat cells were cocultured with the hCD 152 hCD59 or pCD 152 hCD59 expressing cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We investigated the pattern of expression of the B 7 family of immunomodulatory molecules B 7 H1 ( PD L 1 ) , B 7 2 ( CD 86 ) , and B 7 1 ( CD 80 ) at the term maternal fetal interface . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After incubation with either NL 4 3 or JR CSF , IPCs produced a large amount of IFN alpha and at the same time underwent morphological differentiation into DCs with upregulation of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We examined the expression in cryostat sections of scalp skin obtained from a total of 28 patients with AA and from five normal control subjects of ( 1 ) molecules related to the induction of cell death including Fas , Fas ligand ( FasL ) , perforin , granzyme B ( GB ) , and TIA 1 , ( 2 ) molecules related to antigen presentation including CD1a , CD 40 , CD 54 , CD 80 , and CD 86 , and ( 3 ) molecules induced by interferon gamma ( IFN gamma ) including CD 40 , CD 54 , Fas , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 is an important costimulatory molecule for T cells , and it has been shown that cell surface expression of its ligands , CD 80 and CD 86 , can enhance cellular immune responses against tumor cells , however , these tumor cells do not normally express the ligands . ^^^ Many new vaccines will be based upon soluble recombinant antigens , and in vaccination with these antigens CD 80 and CD 86 would normally be expressed on activated antigen presenting cells and additional stimulation through CD 28 would not be predicted to enhance responses further . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In a parallel paper , we had also shown that oral mucosa biopsies fixed in PLP showed good morphology and immunolabeling of CD 54 , CD 80 , CD 83 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expanded cells were low in the activation markers CD 25 , CD 69 , CD 71 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We examined their production of IL 1 beta , IL 10 , IL 12p40 , and IL 12p70 , and their expression of CD 23 , CD 40 , CD 54 , CD 80 , CD 83 , CD 86 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Whereas TSN DCs showed a significant reduction in cell surface expression of CD11c , DEC 205 , MHC class 1 antigen , MHC class 2 antigen , CD 80 , and CD 86 as well as a reduction in the transporter associated proteins , transporter associated with antigen processing 1 and 2 , the changes in phenotype and function were not evident when DCs were cultured in supernatant from COX 2 inhibited tumors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of the co stimulatory molecules , CD 54 , CD 80 and CD 86 , remained unaltered on monocytes treated by ECP . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 binds to its ligand ( CD 80 or CD 86 ) and transduces the most important costimulatory signal . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OM 197 upregulated the expression of HLA DR , CD 80 , CD 86 , CD 83 , CD 40 and CD 54 at the surface of myeloid DC naturally present in blood as well as of DC generated in vitro from monocytes using IL 4 and GM CSF . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upregulation of B 7 molecules ( CD 80 and CD 86 ) and exacerbated eosinophilic pulmonary inflammatory response in mice lacking the IFN beta gene . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Coexpression of 4 1BBL in these E 7 expressing DCs increased effector and memory CTL responses when they were used for immunization . 4 1BBL expression up regulated CD 80 and CD 86 second signaling molecules in DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We demonstrate that MC exosomes induce immature dendritic cells ( DCs ) to up regulate MHC class 2 , CD 80 , CD 86 , and CD 40 molecules and to acquire potent Ag presenting capacity to T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulation with CD 80 and CD 86 expressed on activated dendritic cells was essential for Th cell escape from suppression at a high antigen dose . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This effect on DC was accompanied by neither an up regulation of costimulatory molecules such as B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , and MHC class 2 molecules nor by an induction of cytokine production , including IL 1 , TNF alpha , IL 10 , and IL 12 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , CD11c , CD 40 , CD 80 and CD 86 molecules on DCs were not downregulated after treatment with vincristine either in vitro or in vivo . ^^^ In particular , an upregulated expression of CD 40 , CD 80 and CD 86 molecules on DCs was found in the combination treatment group . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The co stimulatory molecules CD 80 and CD 86 give the secondary signal to T lymphocytes that is necessary for the lysis of leukaemia cells , and CD 40 enhances the efficacy of antigen presentation . ^^^ Here , the expression of HLA ABC , HLA A 2 , HLA DR , CD 40 , CD 80 and CD 86 was flow cytometrically examined in blood samples from 24 healthy volunteers ( HV ) , 24 patients with newly diagnosed acute myeloid leukaemia ( AML ) and five patients with relapsed AML . ^^^ The expression of HLA ABC , CD 40 , CD 80 and CD 86 was significantly reduced on LB in comparison with monocytes of HV . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : hMSCs could be subcultured under this culture condition for 20 passages , expressing CD 13 , CD 29 and CD 59 but not CD 11 , CD 14 , CD 31 , CD 34 , CD 45 , CD 80 , CD 86 , CD 117 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The immunopotentiating capacity of CT and CTB linked antigen was associated with both upregulated secretion of interleukin 1beta by the pulsed DC and increased expression of CD 80 and CD 86 on the DC surface . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expected up regulation of expression of the co stimulatory molecules CD 80 and CD 86 was ablated and that of CD 40 was reduced , as were surface CD 83 expression and intracellular CD 208 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These transduced cells retain the ability to upregulate CD 80 and CD 86 similar to control B cell cultures . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both types of DCs expressed similar levels of CD11c , HLA DR , CD 40 , CD 83 , CD 80 and CD 86 before and after lipopolysaccharide stimulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
VZV infection of mature DCs resulted in a selective downregulation of cell surface expression of the functionally important immune molecules major histocompatibility complex ( MHC ) class 1 , CD 80 , CD 83 , and CD 86 but did not alter MHC class 2 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vitro culture of immature DC generated from adherent peripheral blood mononuclear cells ( PBMC ) using granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 4 ( IL 4 ) with OK 432 at various doses ( 0 . 01 to 0 . 1 KE / ml ) for 2 days resulted in increased cell surface expression of CD 80 , CD 83 , CD 86 and ICAM 1 in a dose dependent manner . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The accessory cell function of ALL blasts showed no correlation with the release of immunomodulatory mediators ( IL 2 , IL 10 , IL 15 ) or the expression of any single adhesion / costimulatory membrane molecule ( CD 54 , CD 58 , CD 80 , CD 86 ) by the blasts . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD11c ( + ) ( F4 / 80 ( ) CD 68 ( ) ) dendritic cells ( DC ) in the colonic lamina propria ( cLP ) of normal and immunodeficient ( RAG 1 ( / ) ) C57BL / 6 ( B 6 ) mice show high surface expression of MHC class I / II molecules and CD1d , and low surface expression of CD 40 , CD 80 , CD 86 costimulator molecules . ^^^ Concomitant with colitis development , DC in the inflamed cLP increase in number and up regulate surface expression of CD1d , MHC class 2 molecules and CD 40 , CD 80 , CD 86 costimulator molecules . cLP DC from non transplanted ( healthy ) and transplanted ( diseased ) mice produce similar amounts of IL 12 p 70 and IL 10 in response to CD 40 signaling , but the inducible IL 12 p 40 release is 5 15 fold higher in mice with colitis than in non transplanted mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have examined the role of CD 40 as an independent costimulatory molecule by generating a panel of transfected human fibroblasts expressing DR 1 with either CD 80 , CD 86 or CD 40 . ^^^ On day 3 the dominant costimulation was provided by CD 40 , while by day 5 this was overshadowed by CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We hypothesized that in relapsing remitting MS ( RRMS ) ( with or without of IFNbeta treatment ) and in secondary progressive patients ( SPMS ) IL 12 and costimulatory molecules ( CD 80 [ B 7 1 ] , CD 86 [ B 7 2 ] , CD 28 , CD 40 , CD40L ) would be differentially produced or expressed by MO or T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We showed that BCG could promote cord blood monocyte derived DC maturation by up regulation of CD 80 , CD 83 , CD 86 , CD 40 , and MHC class 2 molecules and down regulation of mannose receptor . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Preferential expression of B7 . 2 ( CD 86 ) , but not B7 . 1 ( CD 80 ) , on B cells induced by CD40 / CD40L interaction is essential for anti DNA autoantibody production in patients with systemic lupus erythematosus . ^^^ RESULTS : Excessive expression of CD 86 and CD 80 molecules was evident on freshly isolated B cells in patients with SLE . ^^^ Anti DNA antibody production by SLE B cells in the presence of activated T cells was markedly inhibited by anti CD 86 , but not anti CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 , CD 86 , CD 28 and Cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) are well known co stimulatory molecules that form the major co stimulatory pathway essential for full activation of T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This process is characterized by increased expression of CD 80 , CD 86 and MHC class 2 and reduced antigen uptake . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Co stimulatory molecule ( CD 40 , CD 80 and CD 86 ) expression of these cells was deficient in comparison to adult DC , and functional tests revealed these cells to possess a critical absence of CD 40 signalling . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RMEC also express the T cell costimulatory factor CD 58 but not CD 80 , CD 86 , or CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here we show that CD 2 engagement significantly increases class 2 , costimulatory ( CD 40 , CD 80 , CD 86 ) , adhesion ( CD 54 , CD 58 ) , and CCR 7 molecule expression on primary dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : Antibodies to CD 4 , CD 8 , CD 34 , CD 40 , CD 54 , CD 80 , CD 86 , CD 154 , and HLA DR were used for immunohistochemical analysis of lacrimal gland biopsy specimens obtained from nine patients with cGVHD and five with Sjgren ' s syndrome ( SS ) . ^^^ In addition , CD 4 ( + ) and CD 8 ( + ) T cells were colocalized with mononuclear infiltrates and stromal fibroblasts expressing the full component of surface molecules necessary for antigen presentation , including HLA DR , CD 54 , CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fluorescent antibodies specific for CD11b , CD11c , CD 80 , CD 86 , or major histocompatibility complex ( MHC ) class 2 protein each labeled selective populations of cells in vivo . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BACKGROUND : Macrophage ( MPhi ) receptors of the B 7 family ( CD 80 , CD 86 ) play a crucial role in T cell activation : the lack of costimulation leads to anergy or apoptosis of reactive T cells . ^^^ RESULTS : As compared to PBMPhi , CBMPhi showed a significantly decreased upregulation of CD 80 and / or CD 86 after stimulation with IFN gamma , cAMP , CD40L , and alphaCD 3 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 , CD 86 , and CD 40 expression and IL 12 production were more efficient in AdRGD infected mBM DCs than in conventional Ad infected cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 80 , CD 86 and the macrophage marker CD 68 were determined by immunohistochemistry in biopsies from 40 clinical liver transplants obtained at different time points during the transplantation procedure , and in normal liver tissue obtained from 10 human livers . ^^^ Expression of CD 80 and CD 86 on Kupffer cells was graded by comparison with CD 68 staining . ^^^ In a subgroup CD 80 and CD 86 mRNA was quantified by real time detection polymerase chain reaction . ^^^ CD 80 and CD 86 mRNA and protein expression in liver transplants did not change during the warm ischaemic and reperfusion phases of the transplantation procedure . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , stimulation of human monocytes by MPL led to an up regulation of the costimulatory molecules CD 80 and CD 86 , an effect that could be reduced by pretreatment of cells with a monoclonal antibody to TLR 2 or TLR 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Similarly , B cells showed no change in the expression of class 2 major histocompatibility antigens , LFA 1 , ICAM 1 , CD 19 , CD 40 , CD 80 , CD 86 , CD 95 ( Fas ) , or CD 178 ( FasL ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendritic cells ( DCs ) not only induce but also modulate T cell activation . 1 , 25 Dihydroxyvitamin D ( 3 ) [ 1 , 25 ( OH ) ( 2 ) D ( 3 ) ] induces DCs with a tolerogenic phenotype , characterized by decreased expression of CD 40 , CD 80 , and CD 86 co stimulatory molecules , low IL 12 , and enhanced IL 10 secretion . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulation with flagellin induced increased surface expression of CD 83 , CD 80 , CD 86 , MHC class 2 , and the lymph node homing chemokine receptor CCR 7 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
High levels of Fas ligand and MHC class 2 in the absence of CD 80 or CD 86 expression and a decreased CD4+ T cell Infiltration , enables murine skin tumours to progress . ^^^ Detailed flow cytometric analysis was used to study tumour cell expression of FasL , Fas , CD 80 , CD 86 and MHC class 2 . ^^^ The percentage of progressor tumours expressing MHC 2 was significantly greater than regressor tumours , while neither tumour expressed CD 80 or CD 86 costimulatory molecules . ^^^ The results suggest that progression of skin tumours occurs if tumour cells express high levels of MHC 2 but not costimulatory molecules such as CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A Mo AM coculture was exposed to 50 micro g / ml of particles and expression of HLA DR , CD 40 , CD 80 , and CD 86 on each cell type was assessed by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , DCs cultured from CML patients who did not achieve a cytogenetic response to IFN alpha expressed significantly lower levels of class 1 HLA , CD 40 , CD 54 , CD 80 and CD 86 than normal DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Unlike dendritic cells , the CD 4 ( + ) CD 3 ( ) cells have little CD 80 or CD 86 expression but do express high levels of the TNF ligands , OX 40 ligand and CD 30 ligand . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules CD 80 and CD 86 in human crescentic glomerulonephritis . ^^^ BACKGROUND : CD 80 and CD 86 , cell surface molecules found only on antigen presenting cells ( APCs ) , are required for activation of CD 4 postitve ( CD4+ ) T cells by interaction with CD28 / cytotoxic T lymphocyte associated antigen 4 on T cells . ^^^ Expression of CD 80 , CD 86 , CD 4 , CD 14 , CD 68 , HLA DR , and intercellular adhesion molecule 1 was investigated in renal tissues using monoclonal antibodies and compared with clinical data at the time of renal biopsy . ^^^ Tubular epithelial cells showed no CD 86 expression , but they expressed CD 80 , and some of them expressed HLA DR . ^^^ CONCLUSION : This study suggests that the costimulatory molecules CD 80 and CD 86 have different expressions in human crescentic GN , and CD 86 is concerned with crescent formation and CD4+ T cell accumulations . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
MALP 2 and LPS stimulation induced the expression of CD 83 and increased the expressions of CD 80 , CD 86 , HLA ABC and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Tonsil B cells were found to express high amounts of TLR 9 mRNA and protein , and exposure of B cells to CpG ODN but not to an inactive control ODN induced a concentration and time dependent up regulation of the activation markers CD 23 , CD 25 , CD 40 , CD 54 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CX 659S inhibited the expressions of CD 80 and CD 86 , but not that of CD 54 , on Langerhans cells in epidermal cell suspensions . ^^^ Exogenous granulocyte macrophage colony stimulating factor restored the CX 659S induced inhibition of CD 80 and CD 86 expressions of Langerhans cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 4 , CD8alpha , CD 40 , CD 86 , CD 28 , CD 31 and vascular endothelial growth factor was not different between the two tumor groups but expression of CD 40 ligand , CD 80 , NK1 . 1 and CXCR 3 was relatively lower in A11 / Mig tumors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fas ligand ( FasL ) , that has the inherent capacities to tether the T cell inhibitor FasL ( CD 95 ligand ) to the surfaces of B 7 ( CD 80 and CD 86 ) positive APC ( via CTLA 4 : B 7 interaction ) , and in so doing , to simultaneously interfere with B 7 to CD 28 T cell activation signals . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dual expression of CD 80 and CD 86 produces a tumor vaccine superior to single expression of either molecule . ^^^ An aggressive subclone of N2a and the less aggressive parental line were transfected with CD 80 , CD 86 , or both molecules and stable lines were established . ^^^ The less aggressive N2a expressing either CD 80 or CD 86 induced anti tumor immunity . ^^^ In contrast , dual expression of CD 80 and CD 86 was required to initiate a protective anti tumor immune response against the aggressive subclone . ^^^ Control of tumor growth was dependent on CD8+ lymphocytes that infiltrated dual expressing ( CD 80 and CD 86 ) lesions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we investigated the role of this costimulatory pathway in resistance to Trypanosoma cruzi infection by using CD 28 deficient mice and blocking antibodies against CD 80 and CD 86 . ^^^ The blockade of both CD 80 and CD 86 by administering specific monoclonal antibodies also exacerbated T . cruzi infection in wild type mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , we have investigated the expression of costimulatory molecules ( CD 40 , CD 80 , CD 86 , PD 1L , B7H2 , OX40L and 4 1BBL ) in human neuroblastoma ( NB ) cells , since virtually no information is available on this issue . ^^^ Primary NB cells showed CD 40 , CD 80 , CD 86 , OX40L , 4 1BBL , but not PD 1L and B7H2 , mRNA expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Attempts at blocking the CD 28 B7s ( CD 80 CD86 ) pathway are currently underway with the receptor fusion protein , LEA29Y a second generation CTL4Aig , and humanized mAbs to CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The data showed that by the addition of STI 571 the dendritic cells derived from CML clone showed an increased expression of CD1a , CD 83 , CD 80 and CD 86 by flow cytometry analysis and showed more intense abilities of allogeneic antigen presentation by mixed leukocyte culture , compared with the control cells without STI 571 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The modulation of immune cell costimulatory molecules , CD 80 , CD 86 , CD 40 , and HLA DR , and the release of the CD 4 ( + ) T cell chemoattractant IL 16 were also investigated . ^^^ The expression of CD 40 , CD 80 , CD 86 , and HLA DR on monocytes , but not on eosinophils , increased in an RSV dose dependent manner . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Productive infection was associated with downmodulation of cell surface CD 83 , CD1a , CD 80 , CD 86 , ICAM 1 , major histocompatibility complex ( MHC ) class 1 , and MHC class 2 on these cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Berberine did not influence the macrophage cell surface expression of the class 2 major histocompatibility complex molecule , the co stimulatory molecules CD 80 and CD 86 , and intracellular adhesion molecule 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
What ' s the difference between CD 80 and CD 86 . ^^^ Intriguingly , they share two ligands , CD 80 and CD 86 , but at present there is no clear model for understanding whether a ligand will promote or inhibit responses . ^^^ Current perceptions are based around the concept that CD 86 is the initial co stimulatory ligand based on its more abundant and earlier expression pattern ; CD 80 has a role following antigen presenting cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This was not due to down regulation of a number of recognized co stimulatory molecules including CD 80 , CD 86 and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) and CD40L molecules by CD 40 positive ( CD40+ ) and CD 4 positive ( CD4+ ) cells in the lymph nodes of sensitized mice were analysed by flow cytometry ( FACS ) . ^^^ FACS analysis of CD40+ cells in the lymph nodes from sensitized mice showed that simultaneous sensitization with MA and LTA did not enhance CD 80 or CD 86 expression by antigen presenting cells such as B lymphocytes and dendritic cells more than sensitization with MA alone . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Following stimulation via BCR and / or CD 40 surrogate signals for B cells engaged in T dependent signaling differences between the two subsets were seen in the kinetics and / or magnitude of responses such as entry into DNA synthesis , induction of the costimulatory molecules CD 80 and CD 86 ; up regulation of CD 23 , and changes in BCL 6 mRNA expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Reduced CD 80 and CD 86 expression by 1 A ( + ) epidermal cells was also observed . vIL 10 transgenic mice demonstrated a smaller delayed type hypersensitivity response to allogeneic cells upon challenge but had normal contact hypersensitivity to an epicutaneously applied hapten . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
VEGFR 3 ( + ) DC are CD11c ( + ) CD 45 ( + ) CD11b ( + ) , and are mostly major histocompatibility ( MHC ) class 2 ( ) CD 80 ( ) CD 86 ( ) , indicating immature DC of a monocytic lineage . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cross species costimulation : relative contributions of CD 80 , CD 86 , and CD 40 . ^^^ Whereas only porcine CD 86 is constitutively expressed on resting endothelial cells , both CD 40 and CD 80 are rapidly expressed after activation . ^^^ METHODS : We have isolated full length cDNA clones for human and porcine CD 80 , CD 86 , and CD 40 . ^^^ Proliferative responses were of comparable magnitude to those obtained when costimulation was provided by human CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment with humanized monoclonal antibodies against CD 80 and CD 86 combined with sirolimus prolongs renal allograft survival in cynomolgus monkeys . ^^^ We investigated the effect of combining humanized anti CD 80 and anti CD 86 monoclonal antibodies ( mAb ) with sirolimus in cynomolgus monkey renal transplant recipients . ^^^ CONCLUSIONS : Sirolimus can be successfully combined with humanized mAb against CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis revealed that the 14 day old dendritic cells were CD 80 ( + ) , CD 86 ( + ) , CD 83 ( + ) , CD 54 ( + ) , CD1a ( + ) , CD11b ( + ) , CD11c ( + ) , HLA DR ( + ) , CD 34 ( ) , CD 3 ( ) , CD 19 ( ) , CD 14 ( ) , and CD 16 ( ) . ^^^ Reverse transcription polymerase chain reaction was employed to detect expression of mRNA for CD 80 and CD 86 . ^^^ Differentiating monocytes initially expressed CD 86 while CD 80 appeared on day 2 . ^^^ Differentiating stem cells expressed CD 80 and CD 86 on day 2 of culture . ^^^ The surface expression of CD 80 and CD 86 was studied over the course of differentiation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
By examining surface expression of numerous candidate molecules ( tumor necrosis factor receptor [ TNFR ] 1 , TNFR 2 , CD 36 , CD 40 , CD 47 , CD 80 , CD 86 , PD L 1 , CD 95 ligand , and type 1 interferon receptor ) , we report that IPE cells uniquely express on their surface the costimulatory molecule CD 86 . ^^^ When IPE were blocked with anti CD 86 or were derived from CD80 / CD86 ( but not CD 80 ) knockout ( KO ) mice , the cells displayed reduced capacity to suppress T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Following an additional 3 days of culture in the presence of GM CSF , IL 4 , IL 1beta , IL 6 , TNFalpha , and PGE ( 2 ) , cells ( 82 . 0 + / 5 . 8 % CD83+ ) displayed a mature DC morphology and phenotype , including expression of CD11b , CD11c , CD 18 , CD 25 , CD 40 , CD 54 , CD 58 , CD 80 , CD 86 , HLA class 1 , and HLA DR as well as expression of CCR 7 but not CCR 5 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The results showed that gamma irradiation reduced expression of CD 86 , CD 80 and HLA DR molecules on dendritic cells , especially CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The enhanced allo stimulatory capacity of splenic DCs from mice injected with 32DTNF alpha cells correlated with increase in the cell surface expression of the costimulatory molecules CD 40 , CD 80 , CD 86 , and major histocompatibility complex ( MHC ) class 2 molecules ( 1 Ak ) , and production of interleukin 12 ( IL 12 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DCs must express HLA class I / II molecules and the costimulatory molecules CD 40 , CD 80 and CD 86 to effectively activate T cells for the subsequent lysis of leukemic blasts . ^^^ Compared with AML blasts , the expression of CD 40 , CD 80 , CD 86 and HLA DR was upregulated during DC culture to a median of 80 98 % on AML DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Specifically , we measured CD 40 , CD 80 and CD 86 expression , lymphoproliferation and interleukin ( IL ) 4 , IL 10 , interferon ( IFN ) gamma and IL 12Rbeta2 expression in the presence and absence of variant cells and their GXMs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using GM CSF , IL 4 , tumor necrotizing factor ( TNF ) alpha , and CD40L , we could induce mature dendritic cells ( DC ) ( positive for CD 45 , CD 80 , CD 83 , and CD 86 ) from the ventricular fluid in all MC patients . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The functions of the rAAV HbsAg infected DCs were assessed by mixed leukocyte reaction ( MLR ) , and the changes of the surface markers ( including CD 80 , CD 83 , CD 86 and HLA DR ) in response to the infection were detected by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , we show that DC are exquisitely sensitive to their local antigenic microenvironment , and that responses generated by one antigen can be altered by another . 10 IL 2 infection of immature DC led to DC activation ( upregulation of CD 80 , CD 86 and class 2 surface molecules ) when the virus was propagated through xenogeneic , but not syngeneic , mammalian cells ; these DC secreted IL 10 and tumor necrosis factor alpha ( TNF alpha ) , but not IL 12 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Effects of immunoregulatory cytokines ( interferon gamma [ IFN gamma ] and interleukin 10 [ IL 10 ] ) and HSV antigens on B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression on monocytes during HSV 2 infection were investigated using flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD11c+ DCs from the mediastinal lymph nodes of infected mice selectively stimulated both OVA and influenza specific T cells and underwent maturation , with higher levels of MHC class 2 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , higher expression of interferon gamma ( IFN gamma ) in T cells and higher expression levels of , in order of significance , CD 80 ( 2 . 6 to 3 . 8 fold increase ) , CD 86 , and CD 40 on DCs were also observed . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Changes in the number of CD 80 ( + ) , CD 86 ( + ) , and CD 28 ( + ) peripheral blood lymphocytes have prognostic value in melanoma patients . ^^^ Melanoma patients with thinner tumors and those surviving revealed an increase of CD 19 ( + ) CD 80 ( + ) and CD 19 ( + ) CD 80 ( + ) CD 86 ( + ) cells , as well as a higher concentration of CD 3 ( + ) CD 4 ( + ) CD 28 ( + ) cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We monitored the secretion of tumor necrosis factor alpha ( TNF alpha ) and the surface expression of human leukocyte antigen DR and the costimulatory molecules CD 80 and CD 86 on monocytes / macrophages . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , VIP / PACAP reduce the expression of costimulatory molecules ( particularly CD 80 and CD 86 ) on the antigen presenting cells , and therefore reduce stimulation of antigen specific CD4+ T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
PL also enhanced the expression of co stimulatory molecules , CD 80 and CD 86 , in murine B cells in a time dependent manner . ^^^ However , PL has no influence on the [ Ca ( 2+ ) ] concentration and the ROI formation in murine B cells , whereas the genistein , protein tyrosine kinase ( PTK ) inhibitor or staurosporine , protein kinase C ( PKC ) , blocked the proliferation and the induction of co stimulatory molecules , CD 80 and CD 86 , in B cells stimulated with PL . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Treatment with DEX did not affect the expression of surface markers , including H 2Kk , 1 Kk , CD 80 and CD 86 , on DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Strikingly , there was also almost complete ( 92 % ) and selective inhibition of the up regulation of the monocyte costimulatory molecule CD 80 , while 1 CAM 1 , LFA 3 , HLA DR , and CD 86 expression were unaffected . ^^^ They had reduced constitutive expression of CD 40 ( 33 % ) and CD 80 ( 61 % ) , but not CD 86 , and marked inhibition of release of IL 6 ( 72 % ) , IL 12 ( 70 % ) , and TNF alpha ( 46 % ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendritic cell uptake of human apoptotic and necrotic neutrophils inhibits CD 40 , CD 80 , and CD 86 expression and reduces allogeneic T cell responses : relevance to systemic vasculitis . ^^^ RESULTS : Immature DCs engulfed apoptotic and necrotic neutrophils , resulting in up regulation of CD 83 and class 2 major histocompatibility complex molecules , but down regulation of CD 40 , CD 80 , and CD 86 , and a decreased ability to stimulate T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In inflammation , in addition to a significant increase in the number of DCs , a majority up regulated their expression of MHC class 2 , CD 80 , and CD 86 , indicating their state of maturation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Lovastatin inhibited MHC class 2 and CD 40 expression on DC in a dose dependent manner , but had lesser effects on CD 16 , CD 80 , CD 86 , and CD11b expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We analyzed here IgD ( + ) CD 27 ( + ) B cells ( circulating B cells 2 , cB 2 ) and IgD ( ) CD 27 ( + ) memory B cells ( cB 3 ) in comparison with IgD ( + ) CD 27 ( ) naive B cells ( cB 1 ) . cB 2 were found to be morphologically similar to cB 3 with abundant cytoplasm , whereas cB 3 expressed CD 80 , CD 86 , and CD 95 on their surface more predominantly than cB 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
There were no significant differences in the expression of CD11a , CD 40 , CD 80 and CD 86 or MHC classes 1 and 2 molecules between the diagnostic and relapsed samples . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CT also enhanced the expression of CD 80 , CD 86 , and OX 40 ( CD 134 ) on DC and induced the secretion of the chemokine , macrophage inflammatory protein 2 ( MIP 2 ) , but inhibited LPS driven induction of CD 40 and ICAM 1 expression and production of the inflammatory cytokines / chemokines IL 12 , TNF alpha , MIP 1alpha , MIP 1beta , and monocyte chemoattractant protein 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Maturation markers ( i . e . , CD 80 , CD 86 , major histocompatibility complex class 2 , and CD 40 ) showed enhanced expression on DC incubated with targeted PorA liposomes relative to those incubated with nontargeted PorA liposomes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : The effects on expression of HLA DR and costimulatory molecules ( CD 80 , CD 86 , ICAM 1 , and CD 40 ) on normal conjunctival epithelial cells and ChWK treated with various cytokines ( IFN gamma , TNF alpha , IL 4 , and IL 13 ) were determined using flow cytometry and confocal microscopy . ^^^ For ChWK cells , CD 80 and CD 86 were constitutively expressed at low levels . ^^^ CD 80 was significantly upregulated after IFN gamma treatment ( P = 0 . 043 ) , whereas IL 4 induced a significant upregulation of CD 86 ( P = 0 . 039 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Myelin phagocytosing microglia express MHC 2 and the costimulatory molecule CD 86 , but lack CD 80 , which is found only on activated antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Retrovirus transfected bone marrow cells cultured with GMCSF and IL 4 for 7 days demonstrated 70 80 % of DCs with high CD11c , CD 80 , CD 86 , and MHC class 1 ( 1 Kd ) expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 , but not CD 86 were up regulated on the spleen derived dendritic cells from OVA sensitized and challenged BALB / c mice . ^^^ There is increasing evidence that CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , as the most important costimulatory molecules , are involved in the allergic immune responses . ^^^ In the present study , we investigated the CD 80 and CD 86 expression of spleen derived dendritic cells ( DCs ) in a murine model of allergic asthma . ^^^ In this model , we found that CD 80 were significantly upregulated on the spleen derived DCs from OVA sensitized and challenged mice compared with that from PBS treated or non treated mice , while CD 86 is not different among three groups . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , decidual DCs expressed costimulatory molecules such as CD 80 and CD 86 and a mature marker such as CD 83 on their surface . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The results showed that CD 22 , CD 20 , CD62L , CD 40 , CD 24 , CD79b , CD79a , and FMC 7 were almost positive in the circulating B cell population , whereas CD11a , CD 80 , CD 103 , CD 10 , CD40L , CD 54 , CD95L , CD 86 , and CD 95 were almost negative in the peripheral blood B lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Most importantly , immunophenotypic analyses demonstrated that MPCs did not express major histocompatibility complex class 2 molecules or the T cell costimulatory molecules CD 80 and CD 86 , consistent with further investigation showing that MPCs failed to elicit a proliferative response from allogeneic lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : The long term culture of splenocytes resulted in the generation of a cell line , termed NOD DC 1 , which have a phenotype of myeloid DCs ( CD11c , CD11b , DEC 205 ) , express MHC class 2 and co stimulatory molecules ( CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The presence of GSNO during maturation of DCs caused a reduced expression of surface CD 86 , whereas CD 80 , CD 83 , and MHC molecule expression was not affected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Parent OM selectively up regulated Toll like receptor 4 ( TLR 4 ) mRNA expression and induced mo DC maturation , as reflected by increased production of chemokines , proinflammatory cytokines , and CD 83 , CD 80 , CD 86 , CD 40 , and major histocompatibility complex ( MHC ) class 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD 80 or CD 86 costimulatory molecules on tumor cells can produce rejection of immunogenic but not poorly immunogenic tumors . ^^^ Anti CD 3 receptors effectively induced T cell cytotoxicity , whereas coexpression with CD 80 or CD 86 was required for generating T cell proliferation and IL 2 secretion . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We first observed that neonatal pDCs displayed decreased up regulation of CD 80 , CD 83 , CD 86 , and CD 40 , whereas HLA DR and CD 54 up regulation did not differ significantly between adults and neonates . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On the other hand , however , and in contrast to the effects of IL 10 on human DC , we found that the addition of IL 10 from the initiation of the culture onwards induced an up regulation of the expression of the costimulatory molecules CD 40 , CD 80 and CD 86 on murine myeloid DC , as compared to DC generated with GM CSF only . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
TNF alpha markedly increased the expression on DC of major histocompatibility complex ( MHC ) and the costimulatory molecules , CD 40 , CD 80 and CD 86 . ^^^ Both NAC and GSH completely abolished the TNF alpha induced enhancement of CD 40 expression , but had no considerable effect on the expression of CD 80 , CD 86 and MHC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Administration of dendritic cells transduced with antisense oligodeoxyribonucleotides targeting CD 80 or CD 86 prolongs allograft survival . ^^^ We examined the effects of transduction with high affinity antisense oligodeoxyribonucleotides ( ODNs ) designed to target the mRNA of CD 80 or CD 86 on the phenotype and function of dendritic cells ( DCs ) . ^^^ MATERIALS AND METHODS : DCs were propagated from C57BL / 10 ( B 10 ; H2b ) bone marrow cells in granulocyte macrophage colony stimulating factor and interleukin ( IL ) 4 , and transduced with anti CD 80 or anti CD 86 antisense ODNs ( base mismatched ODNs as controls ) . ^^^ Antisense ODNs targeting CD 80 or CD 86 mRNA specifically suppressed the expression of CD 80 or CD 86 in DCs and inhibited their capacity to elicit the proliferative responses , donor specific cytotoxic T lymphocyte activity in C3H ( H2k ) spleen T cells . ^^^ In contrast with the administration of control DCs into C3H recipients that exacerbated rejection of B 10 cardiac allografts , injection of DCs transduced with anti CD 80 or CD 86 antisense ODN significantly prolonged the survival of heart allografts . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These changes correlated with down regulation of co stimulatory and adhesion molecules such as CD 40 , CD 54 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
At 12 h postinfection , wt HSV 2 upregulated DC major histocompatibility complex ( MHC ) class 2 expression , and in contrast to UV inactivated HSV 2 , downregulated expression of MHC class 1 , but it had no effect on surface CD 40 , CD 80 , or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The kinetics of CD 80 and CD 86 expression on antigen presenting cells in cedar pollinosis subjects ] . ^^^ CD 80 and CD 86 , which are costimulatory molecules in T cell activation , play important roles in the differentiation of Th 1 or Th 2 phenotypes . ^^^ The results of blocking studies using neutralizing antibodies have suggested that CD 80 and CD 86 also play important roles in sensitization to cedar pollen antigen , but very few studies have examined the kinetics of CD 80 and CD 86 expression on antigen presenting cells ( APC ) . ^^^ We studied the kinetics of CD 80 and CD 86 expression on APC after allergen stimulation in cedar pollinosis subjects . ^^^ Zero to 8 days following in vitro stimulation , the expression of CD 80 and CD 86 in either CD14+ or CD19+ cells was analyzed by two color flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Analysis of intragraft gene and protein expression of the costimulatory molecules , CD 80 , CD 86 and CD 154 , in orthotopic liver transplant recipients . ^^^ Gene and protein expression of CD 80 , CD 86 and CD 154 were analyzed by RT PCR and immunohistochemistry . ^^^ In reperfusion biopsies CD 154 , but not CD 80 or CD 86 , protein expression correlated with the total ischaemic time . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , CCL 16 activated macrophages overexpress membrane CD 80 , CD 86 , and CD 40 costimulatory molecules and extensively phagocytose tumor cell debris . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although IFN DCs had a more mature immunophenotype than IL 4 DCs , showing higher expression of CD 80 , CD 86 , and CD 83 , they still preserved comparable endocytic and phagocytic capacities and responsiveness to maturation stimuli . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both fungal forms induced DC maturation , as indicated by up regulation of CD 83 , CD 80 , CD 86 , CD 40 , and major histocompatibility complex classes 1 and 2 surface antigens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Dendritic cells from healthy ( DC Hs ) or MC 38 tumour bearing mice ( DC TBs ) were examined for endocytotic capacity of FITC labelled dextran , antigen presenting capacity in allogeneic mixed leucocyte reaction ( allo MLR ) and expression of 1 Ab , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The macrophages express elevated levels of cell surface molecules CD 80 , CD 86 , CD 54 and MHC 2 , markers characteristic of antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Transduction had no effect on EBV LCL expression of major histocompatibility complex ( MHC ) antigens or of costimulatory molecules CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Augmentation of CTL responses correlated with up regulation of CD 80 and CD 86 expression in DCs transduced with costimulatory molecules , suggesting a mechanism for enhanced T cell activation / survival . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In both the influenza and HIV DNA immunization models , several groups have reported that coimmunization of mice with plasmids encoding immunogen and CD 86 , but not CD 80 , effectively boosts Ag specific T cell activation . ^^^ To examine this issue , we developed a series of chimeric CD 80 and CD 86 constructs as well as deletion mutants , and examined their immune activating potential in the DNA vaccine model . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The addition of active PSA to DC cultures significantly inhibited the generation and maturation of DC , as assessed by the levels of expression of CD 83 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this work , we examined if resistance of a protein to proteolytic processing affects the expression of costimulatory molecules , CD 80 and CD 86 , on macrophage exposed to the same antigen . ^^^ The murine peritoneal cavity macrophages expressed both CD 80 and CD 86 after 24 h of incubation with HlyA monomer but failed to express the costimulatory molecules when treated with the HlyA oligomer . ^^^ The expression of CD 80 molecule on macrophage after 48 h by the HlyA oligomer that failed to express the costimulatory molecules after 24 h indicates that proteolytic processing plays a decisive role in expression of CD 80 and CD 86 on cell surface . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After culture for 20 hours in vitro , SF neutrophils from RA patients expressed large amounts of surface class 2 MHC but very low levels of costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Effects of CD 80 and CD 86 on cytokine production in patients with wasp venom allergy who receive venom immunotherapy . ^^^ Several studies have provided evidence that activation of antigen specific T cells requires interactions between CD 28 on T cells and its ligands , CD 80 and CD 86 , on antigen presenting cells ( APCs ) . ^^^ However , the effects of CD 80 and CD 86 on cytokine production in patients with Hymenoptera venom allergy who receive venom immunotherapy remain unclear . ^^^ We examined the effects of CD 80 and CD 86 on Th 1 and Th 2 cytokine production before and after venom immunotherapy in patients with wasp venom allergy . ^^^ CD4+ T cells and monocytes were isolated as APCs from PBMCs and were cocultured with wasp venom in the presence of anti CD 80 or CD 86 blocking antibodies . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Supplementation of the formula with TGF beta 2 down regulated the proinflammatory cytokine mRNA as well as the number of activated lymphocytes , eosinophils , mast cells , CD 80 , and CD 86 positive dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Surviving rats were euthanized on day 150 and intragraft gene ( CD 80 , 86 , 152 , 154 , IFN gamma , IL 2 , IL 6 , IL 10 , IL 13 , TNF alpha , TGF beta , IL 7 , Fas ligand , Granzyme B , bax , and bcl ( 2 ) ) and protein ( CD 4 , CD 8 , ED 1 , CD 154 , CD 80 , CD 86 ) expression was measured . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mature DC showed a high expression of CD 83 , HLA DR , and the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Importantly , Ficoll treated monocytes retained their ability to differentiate to mature DCs demonstrated by morphology , phenotype ( MHC class 2 ( + ) , CD1a ( + ) , CD 80 ( + ) , CD 86 ( + ) , and CD 83 ( + ) ) , ability to stimulate mixed lymphocyte responses ( MLR ) , present antigen , and produce interleukin 12 ( IL 12 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The present study examined the expression of a range of co stimulatory molecules : CD 40 , CD 54 , CD 80 , CD 86 and HLA DR , on the cell surface of CD 14 ( + ) peripheral blood monocytes ( PBM ) from relapsing remitting ( RR ) and secondary progressive ( SP ) MS patients , prior to and during 1 year of Interferon ( IFN ) beta 1a ( Rebif ( R ) ) therapy . ^^^ IFN beta therapy led to a significant reduction in the expression of CD 54 and CD 80 in both groups of patients as well as to elevation of CD 40 and CD 86 expression in SP patients . ^^^ These results confirm IFN mediated modulation of the APC surface within the immunological synapse and implicate CD 80 and CD 86 as targets for interventional therapies in MS as well as other Th 1 mediated autoimmune diseases . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Activation of the innate immune system allows the activation of the adaptive immune response by production of proinflammatory cytokines and by providing stimulatory signals via major histocompatibility complex molecules and costimulatory molecules such as CD 40 , CD 80 , or CD 86 ; together , these lead to the full activation of both immune systems to fight against pathogenic microorganisms . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
One group of mice was vaccinated with an irradiated , 410 . 4 syngeneic mammary tumor cell line co expressing human MUC 1 and CD 80 or CD 86 co stimulatory molecules , and a second group of mice was vaccinated with plasmids encoding MUC 1 and CD 80 or CD 86 . ^^^ There were significant inhibition on rates of tumor growth and survival in mice vaccinated with irradiated 410 . 4 / MUC1 cells co expressing either CD 80 or CD 86 molecules , compared to non vaccinated animals . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We performed a phase I / II clinical trial in metastatic melanoma patients with an ultraviolet ( UV ) inactivated nonreplicating recombinant vaccinia virus enabling the expression , from a single construct , of endoplasmic reticulum targeted HLA A 0201 restricted Melan A / MART 1 ( 27 35 ) , gp 100 ( 280 288 ) , and tyrosinase ( 1 9 ) epitopes , together with CD 80 and CD 86 costimulatory proteins . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mice transgenic for the HER 2 / neu homologue , rat neu , were immunized with full length rat neu cDNA given alone or in combination with plasmids encoding costimulatory molecules CD 80 or CD 86 and the ligand for CD 137 ( CD137L ) . ^^^ Immunization with plasmids encoding the neu antigen along with plasmids encoding CD137L and either CD 80 or CD 86 resulted in the generation of neu specific antibodies that induced phopshorylation of the neu tyrosine kinase and inhibited the growth of cultured tumor cells overexpressing neu . ^^^ DNA vaccines encoding neu , when given in combination with both CD137L and either CD 80 or CD 86 , can induce cellular and humoral immunity and result in an antitumor effect . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This conclusion was based on the similar phenotype profile of DC , such as the expression of CD1a , CD 80 , CD 86 , and HLA DR , down regulation of CD 14 , and the absence of CD 83 , as well as on their similar allostimulatory activity for T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Function of CD 80 and CD 86 on monocyte and stem cell derived dendritic cells . ^^^ Reverse transcription polymerase chain reaction revealed that differentiating monocytes initially expressed CD 86 mRNA while CD 80 mRNA appeared on Day 2 . ^^^ Differentiating stem cells expressed both CD 80 and CD 86 mRNA on Day 2 of culture . ^^^ Monoclonal antibodies ( mabs ) to CD 80 and CD 86 were employed to assess their costimulatory roles . ^^^ A decrease in stimulation as depicted by decreased T cell activation was significant with mabs to both CD 80 and CD 86 on monocyte derived DCs while only mabs to CD 86 induced decreased T cell activation by stem cell derived DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA4Ig binds to CD 80 and CD 86 on antigen presenting cells , blocking the engagement of CD 28 on T cells and preventing T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Porins and lipopolysaccharide from Salmonella typhimurium regulate the expression of CD 80 and CD 86 molecules on B cells and macrophages but not CD 28 and CD 152 on T cells . ^^^ RESULTS : Our results show that porins of S . typhimurium increase the expression of CD 86 and the expression of CD 80 both on B lymphocytes and macrophages , while the expression of CD 28 and CD 152 on T lymphocytes was unaltered . ^^^ The expression of CD 80 and CD 86 is dose dependent and starts after 24 h post treatment , peaks at 48 h and goes back to the basal value after 72 h . ^^^ CONCLUSIONS : S . typhimurium porins are able to induce a high expression of costimulatory molecules ( CD 80 and CD 86 ) on lymphocytes and macrophages . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We observed that neonatal myeloid DC displayed decreased upregulation of CD 40 , CD 80 whereas CD 86 and HLA DR upregulation did not differ significantly between adults and neonates . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , a downexpression of activation markers ( CD 25 , CD 21 , CD 80 , CD 86 ) on B cells characterized the group MB 1 patients and was associated with an upexpression of activation markers ( HLA DR , CD 95 , CD 57 ) on T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast to CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) , ICOSL protein and mRNA was expressed in 7 of 12 glioma cell lines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of MHC Class 2 , CD 70 , CD 80 , CD 86 and pro inflammatory cytokines is differentially regulated in oral epithelial cells following bacterial challenge . ^^^ Expression of cell surface molecules ( major histocompatibility complex ( MHC ) Class 2 , CD 54 , CD 70 , CD 80 and CD 86 ) and secretion of inflammatory cytokines ( interleukin ( IL ) 1beta , IL 6 , and tumor necrosis factor ( TNF ) alpha ) in response to selected bacterial challenge were examined on an immortalized oral epithelial cell line , HOK 18A and a skin epithelial cell line , HaCaT . ^^^ Subsequently , cell surface expression of MHC Class 2 , CD 54 , CD 70 , CD 80 and CD 86 , along with pro inflammatory cytokine levels were determined using flow cytometry , ELISA and RT PCR . ^^^ Interestingly , CD 70 , CD 80 , and CD 86 were generally decreased upon bacteria and LPS challenge on HOK 18A . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OK 432 increased expression of activation and maturation related molecules such as HLA DR , CD 80 , CD 83 , and CD 86 in imMo DCs at levels similar to that of TNF alpha plus PGE 2 , and higher than that of LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 86 and CD 80 costimulatory antigens are highly overexpressed on Hodgkin / Reed Sternberg cells in patients with Hodgkin ' s disease ( HD ) and are candidate target antigens for immunotoxins in order to eliminate minimal residual disease . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The generated matured transfected DCs show high expression of the antigens CD 83 , CD 80 , CD 86 and human leucocyte antigen DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
TNF alpha markedly increased the surface expression of major histocompatibility complex ( MHC ) and costimulatory molecules , CD 86 and CD 80 , on DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
They lost the progenitor markers CD 33 , CD 13 and CD 34 while significantly increasing expression of the co stimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fc on the differentiation of DCs was the up regulation of CD 86 and down regulation of CD 80 , suggesting a modulatory potential to skew the T cell response toward the T helper cell type 2 ( Th 2 ) phenotype . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Protection depended on the presence of costimulatory molecules CD 80 , CD 86 , and CD 40 on the DCs , but surprisingly did not require DCs that could make IL 12 or IL 15 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , it profoundly increased CD 80 , CD 86 , MHC 1 , and MHC 2 expression in murine , GM CSF and IL 4 stimulated , BM derived myeloid DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Porin treatment of CD11b+ peritoneal cavity ( PerC ) MPhi of BALB / c mouse was found to up regulate CD 80 on cell surface and had no effect on CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : By flow cytometry , tape stripping augmented the expression of MHC class 2 , CD 54 , CD 80 , CD 86 and CD 40 on Langerhans cells , whereas UVB decreased the expression of some of these molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BOM , NMB and GRP dose dependently inhibited maturation of DC assessed as down regulation of CD 40 , CD 80 and CD 86 expression on DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment of IEC 6 cells with GM CSF enhanced expression of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The cell surface expression of CD 24 , CD 40 , CD 54 , CD 80 , CD 86 , OX40L and MHC class 2 molecules was barely altered following infection with unopsonized promastigotes or amastigotes from nude mice or with C 3 coated promastigotes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , DC derived from acute myeloid leukemia blasts did not show an elevated level of ACE , although they acquired DC markers CD 80 , CD 40 , and CD 86 upon cytokine or calcium ionophore treatment . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 18 strongly up regulated CD 54 on MoDC , whereas the effect on MHC class 2 , CD 83 , and CD 86 was only moderate and the expression of CD 40 and CD 80 was not affected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This is not due to alterations in Ag processing or to an increase in surface expression of CD 80 , CD 86 , ICAM 1 , or MHC 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A lesser expression of costimulatory molecules ( CD 80 and CD 86 ) was seen in DCs treated with exogenous VEGF than in DCs not treated with VEGF . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
EGCG , in a dose dependent manner , profoundly inhibited CD 80 , CD 86 , and MHC class 1 and 2 expression on bone marrow derived murine myeloid DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Herein , we report that Ebola VLPs ( eVLPs ) were immunogenic in vitro as eVLPs matured and activated mouse bone marrow derived dendritic cells , assessed by increases in cell surface markers CD 40 , CD 80 , CD 86 , and MHC class 1 and 2 and secretion of IL 6 , IL 10 , macrophage inflammatory protein ( MIP ) 1alpha , and tumor necrosis factor alpha by the dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Given the critical role of HLA molecules and the costimulatory signals delivered by CD 80 and CD 86 in optimal antigen presentation and T cell activation , inhibition of expression of HLA and CD80 / CD86 on DCs by IVIG offers a plausible explanation for the efficacy of IVIG in SLE and other immune mediated inflammatory conditions . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
HA treatment upregulated the expression of costimulatory molecules such as CD 40 , CD 80 , and CD 86 on DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It expresses MHC 1 and CD95 / Fas , while HLA DR , CD 40 , CD 80 , CD 86 , MUC 1 , MUC 2 , and ICAM 1 / CD54 are absent . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 expression on LPS stimulated monocytes and the effect of CD 80 and CD 86 blockade on IL 4 and IFN gamma production in nonatopic bronchial asthma . ^^^ CD 80 and CD 86 seem to play an important role in the allergen induced secretion of interleukin ( IL ) 5 and IL 13 . ^^^ Up to now , the expressions of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) on monocytes and the kinetics of the expression of these molecules on lipopolysaccharide stimulated monocytes in nonatopic asthma have not been defined . ^^^ Using monoclonal antibodies , we have compared the expressions of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) on the monocytes of healthy persons and nonatopic asthmatic patients . ^^^ We have also assessed the effect of CD 80 and CD 86 inactivation on IL 4 and interferon ( IFN ) gamma production in nonatopic asthmatics and healthy subjects . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The soluble Id CD40L fusion protein retained CD 40 binding activity and stimulated CD 80 and CD 86 upregulation and interleukin ( IL ) 12 production by macrophages . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Presence of spinal B7 . 2 ( CD 86 ) but not B7 . 1 ( CD 80 ) co stimulatory molecules following peripheral nerve injury : role of nondestructive immunity in neuropathic pain . ^^^ Recent studies suggest that co stimulatory molecules B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) play a differential role in the effect of beneficial versus deleterious CNS autoimmune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
First , using interleukin 4 ( IL 4 ) / granulocyte macrophage colony stimulating factor ( GM CSF ) induced , blood monocyte derived DC , hapten induced up regulation of maturation / activation markers , including CD 80 , CD 83 , CD 86 , chemokine receptors CXCR 4 and CCR 5 , as well as the drug resistance related molecules P glycoprotein ( Pgp ) and lung resistance protein ( LRP ) , were monitored by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Conversely , the lack of the dominant costimulatory molecules , CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) could be a possible explanation of poor immunogenicity of OVCA tumors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : Airway eosinophils were recovered from ovalbumin sensitized and challenged BALB / c mice , these eosinophils were then cocultured with sensitized CD 4 ( + ) cells in the absence or presence of anti CD 80 or / and CD 86 monoclonal antibodies . ^^^ RESULTS : Our data showed that airway eosinophils , recovered following inhalational ovalbumin challenge in sensitized mice functioned as CD 80 and CD 86 dependent antigen presenting cells to stimulate sensitized CD 4 ( + ) lymphocytes to produce IL 4 , IL 5 and IL 13 , but not interferon gamma ( IFN gamma ) in vitro assay . ^^^ When instilled intratracheally in ovalbumin sensitized recipient mice , these antigen loaded eosinophils migrated into draining paratracheal lymph nodes primed Th 2 cells in vivo for IL 4 , IL 5 and IL 13 , but not IFN gamma , production during the in vitro culture that was also CD 80 and CD 86 dependent . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The B 7 family of molecules on antigen presenting cells , which include B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , play important roles in providing costimulatory signals required for development of antigen specific immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
During migration , DC upregulated the expression of major histocompatibility complex 2 and costimulatory receptors ( such as CD 40 , CD 80 , and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The authors observed that DC increased their expression of CD 80 , CD 86 , CD 40 , and human leukocyte antigen ( HLA ) DR and neoexpressed CD 83 , DC LAMP / CD208 , and CCR 7 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here we compared untreated MS , therapy with IFN beta alone and combined with GA , and healthy controls ( HC ) , regarding expression on HLA DR+ blood mononuclear cells ( MNC ) of CD1a that is a cell surface molecule with capacity to present glycolipids to T cells , and of CD 80 and CD 86 which are costimulatory molecules that activate Th 1 and Th 2 responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The latter DC population could be subdivided into a major , immature ( CD 40 ( lo ) CD 80 ( lo ) CD 86 ( lo ) MHC class 2 ( lo ) ) CD11c ( int ) subset , and a minor , mature ( CD 40 ( hi ) CD 80 ( hi ) CD 86 ( hi ) MHC class 2 ( hi ) ) CD11c ( hi ) subset . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The role of B 7 ligands ( CD 80 and CD 86 ) in CD 152 mediated allograft tolerance : a crosscheck hypothesis . ^^^ BACKGROUND : The regulatory mechanism by which the B 7 ligands ( CD 80 and CD 86 ) direct the CD28 / CD152 costimulatory pathways is unclear . ^^^ This study investigated the role of CD 80 and CD 86 in a CD 152 mediated allograft tolerance model . ^^^ The influence of anti CD 80 and anti CD 86 treatments on the proliferation and interleukin ( IL ) 2 productions of the tolerated splenocytes ( SC ) was analyzed . ^^^ The role of CD 80 and CD 86 in the induction and maintenance of the graft acceptance in this transplant model were also tested . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Then , the expression of HLA DR , CD 40 , CD 44 , CD 63 , CD 80 , CD 86 , Fas receptor , E cadherin , ICAM 1 , MUC 1 , cytokeratins and vimentin were investigated by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : DCs transfected and matured had increased cell surface expression of CD 40 and costimulatory molecules CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Effects of preactivated autologous T lymphocytes on CD 80 , CD 86 and CD 95 expression by chronic lymphocytic leukemia B cells . ^^^ CLL B cell expression of CD 86 and CD 95 was markedly upregulated using this approach , whereas CD 80 expression was augmented only in a minority of patients ; these effects were partially preserved even when preactivated T cells were rechallenged with CLL B cells at the same low T / B cell ratio as that observed in vivo . ^^^ Finally , CD 80 upregulation on CLL B cells appeared to be mainly dependent on CD40L mediated stimulation , whereas CD 86 and CD 95 expression was efficiently augmented by soluble factors released by preactivated T lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells displayed morphological and phenotypic features of myeloid DC precursors with expression of HLA DR , HLA ABC , CD 86 , CCR 5 and CD 54 molecules but lacking CD 80 , CD 83 , CD1a and CD 40 antigens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Monoclonal antibodies were used to assess the expression of CD 80 , CD 86 , CD 68 , CD 14 , CD45RO , human leukocyte antigen DR ( HLA DR ) , and intercellular adhesion molecule 1 ( ICAM 1 ) in renal tissues . ^^^ CONCLUSION : This study suggested that CD 80 and CD 86 activate T cells in IgAN , CD80 / CD86 expressions correlated with renal function at the time of renal biopsy , and monocyte / macrophages and tubular epithelial cells act as APC . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
PGE ( 2 ) as well as 11 , 15 O dimethyl prostaglandin ( E ( 2 ) ONO AE 1 259 01 ) ( EP 2 receptor agonist ) and ONO AE 1 329 ( EP 4 receptor agonist ) concentration dependently enhanced the expression of CD 80 , CD 86 , CD 83 , and HLA DR on MoDCs during maturation , especially in the presence of TNF alpha , whereas 17S 2 , 5 ethano 6 oxo 17 , 20 dimethyl prostaglandin E ( 1 ) ( EP 1 receptor agonist ) and 16S 9 deoxy 9beta chloro 15 deoxy 16 hyfroxy 17 , 17 trimethylene 19 , 20 didehydro prostaglandin F ( 2 ) ( EP 3 receptor agonist ) showed no effect . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of CD1a , CD 80 , CD 86 in dendritic cell of tumor tissue and regional lymph node in esophageal carcinoma ] . ^^^ This study was designed to investigate the expression of CD1a ( specific marker ) and CD 80 , CD 86 ( costimulating molecules ) in dendritic cell of tumor tissue and regional lymph node of esophageal carcinoma and to investigate the mechanisms by which tumors escape immune recognition . ^^^ METHODS : The expression of CD1a , CD 80 , CD 86 in dendritic cells of tumor tissues and paratumor tissues from 58 patients with esophageal carcinoma ( clinical period 1 2 : 27 cases , 3 4 : 31 cases ; differentiation G ( 1 2 ) : 37 cases , G 3 : 21 cases ; squamous cancer : 49 cases , adenoid cancer : 9 cases ; lymph node metastases present : 37 cases , lymph node metastases absent : 21 cases ) , inflammatory tissues from 11 patients with esophagitis , 12 normal esophageal tissues ( as control ) , 31 regional lymph nodes with tumor and 34 regional lymph nodes without tumor were determined using flow cytometry . ^^^ CONCLUSION : ( 1 ) The number of dendritic cell of CD1a ( + ) decreases in tumor tissue and regional lymph node with tumor in esophageal carcinoma and the expression of CD 80 , CD 86 are down regulated . ( 2 ) The lower the tumor differentiation grade , the less the number of CD1a ( + ) dendritic cell in tumor tissue and regional lymph node and the lower the expression of CD 80 , CD 86 in tumor tissue . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Indirect recognition predominated the response , as antibodies against mouse class 2 1 A ( b ) , CD 80 , or CD 86 molecules significantly ( 45 to 60 % ) blocked the proliferative response . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Importantly , they become fully competent antigen presenting cells ( APC ) after a short maturation induced by IL 3 + CD40L or virus , exhibiting a characteristic APC phenotype ( high expression of CD 83 and of the costimulatory molecules CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulation of DCs with S . pyogenes resulted in strong expression of DC costimulatory molecules CD 80 , CD 83 , and CD 86 accompanied with a T helper cell type 1 ( Th 1 ) cytokine and chemokine response . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cross regulation of CD 86 by CD 80 differentially regulates T helper responses from Mycobacterium tuberculosis secretory antigen activated dendritic cell subsets . ^^^ We report that stimulation of Mycobacterium tuberculosis secretory antigen and tumor necrosis factor alpha matured BALB / c mouse bone marrow dendritic cells ( BMDCs ) with anti CD 80 monoclonal antibody up regulated CD 86 levels on the cell surface . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells expressed significant levels of CD 40 , CD 80 and CD 86 , and could stimulate allogeneic CD4+ T cells efficiently . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Finally , CD 80 and CD 86 , molecules involved in T B cooperation , showed an inverse expression between lymphocytes of B CLL patients and healthy subjects . ^^^ CD 80 was higher on normal than leukemic B cells , while CD 86 expression was higher on CLL B cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vitro study revealed that CD 80 , CD 86 and CD11b expression on macrophages was augmented after IL 1beta stimulation , whereas the up regulation could be blocked by sodium salicylate . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The coculturing of iDCs with apoptotic cells did not induce the maturation of iDCs even after the subsequent LPS treatment , as assessed as to the expression of MHC class 2 , CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Myeloid DC from hepatic LN showed similar expressions of HLA DR , CD 83 , and CD 86 , and higher expression of CD 80 compared to myeloid DC from inguinal LN . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory molecules ( CD 80 , CD 86 , CD 28 , CD 152 ) , accessory molecules ( TCR alphabeta , TCR gammadelta ) and T cell lineage molecules ( CD4+ , CD8+ ) in PBMC of leprosy patients using Mycobacterium leprae antigen ( MLCWA ) with murabutide and T cell peptide of Trat protein . ^^^ This observation prompted us to study the expression of the costimulatory molecules ( CD 80 , CD 86 , CD 28 , CD 152 ) , other accessory molecules ( TCR alphabeta / gammadelta ) and T cell lineage molecules ( CD4+ and CD8+ ) during constitutive and activated state of peripheral blood mononuclear cells ( PBMC ) derived from normal and leprosy individuals using different formulations of Mycobacterium leprae total cell wall antigen ( MLCWA ) , Trat and MDP BE using flow cytometric analysis . ^^^ An increased surface expression of CD 80 , CD 86 and CD 28 but decreased CD 152 expression was observed when PBMC of normal , BT / TT ( tuberculoid ) and BL / LL ( lepromatous ) patients were stimulated in vitro with MLCWA+MDP BE+Trat peptide using liposomal mode of antigen delivery , while opposite results were obtained with the antigen alone . ^^^ Antibody inhibition study using antihuman CD 80 or CD 86 completely abolished the T cell lymphoproliferation , thereby reconfirming the importance of these costimulatory molecules during T cell activation / differentiation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis showed that HOT stimulated DC to express the surface maturation markers CD 80 , CD 83 , and CD 86 dose dependently and that the up regulation level was identical to TNF alpha and LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have therefore investigated associations between MS and polymorphisms in the CD 152 ( CTLA 4 ) , CD 28 , CD 80 and CD 86 genes in Australian patients . ^^^ Screening of CD 28 , CD 80 and CD 86 genes identified novel polymorphisms in the putative promoter regions of CD 28 ( 372 G / A ) and CD 86 ( exon 2 359 deletionAAG ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CyaA also enhanced cell surface expression of CD 80 , CD 86 , and major histocompatibility class 2 on immature DC . ^^^ The stimulatory activity of our CyaA preparation for IL 10 production and CD 80 , CD 86 , and major histocompatibility complex class 2 expression was attenuated following the addition of polymyxin B or with the use of DC from Toll like receptor ( TLR ) 4 defective mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 86 and CD 80 differentially modulate the suppressive function of human regulatory T cells . ^^^ As both CD 28 and CTLA 4 molecules are implicated in the function of Treg , we investigated the ability of their two natural ligands , CD 80 and CD 86 , to influence the Treg suppressive capacity . ^^^ Intriguingly , the relative expression levels of CD 80 and CD 86 on dendritic cells are modulated during progression from an immature to a mature state , and this correlates with the ability of Treg to suppress responses . ^^^ Our data show that CD 80 and CD 86 have opposing functions through CD 28 and CTLA 4 on Treg , an observation that has significant implications for manipulation of immune responses and tolerance in vivo . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cultured bone marrow nucleated cells expressed dendritic cell markers including MHC class 2 antigen , CD 80 , CD 86 , and CD11c . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , inosine did neither influence membrane expression of CD 54 , CD 80 , CD 83 , CD 86 , HLA DR , and MHC class 1 molecules nor modulated secretion of interleukin ( IL ) 12 , IL 10 , and tumor necrosis factor alpha in immature and lipopolysaccharide matured DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory molecules , CD49D and CD 28 on T cells and CD 80 and CD 86 on monocytes , was unchanged . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After 7 d , the majority of cells showed DC like morphology , and expressed higher levels of CD 80 , CD 86 and HLA DR than fresh leukaemic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The results indicated that gamma irradiation reduced expression of CD 86 , CD 80 and HLA DR molecules on DC , especially CD 86 ( P=0 . 0072 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 , CD 86 ) and adhesive molecules ( eg . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Only TNF alpha stimulated EC induced the development of mature , allostimulatory DC with a high expression of CD 83 , HLA DR , CD1a , and costimulatory molecules like CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The immunosuppressive LCMV variant clone 13 ( Cl 13 ) infects DCs and interferes with their maturation and antigen presenting capacity as evidenced by a significant reduction in the surface expression of MHC class 1 , MHC class 2 , CD 40 , CD 80 , and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vitro stimulation of immature murine DC with MALP 2 resulted in the induction of maturation with up regulated expression of MHC class 2 , costimulatory ( CD 80 , CD 86 ) and adhesion ( CD 40 , CD 54 ) molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Resveratrol inhibited the expression of costimulatory molecules ( CD 80 and CD 86 ) , and major histocompatibility complex ( MHC ) classes 1 and 2 significantly , and had the same effect dose dependently on DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We observed a weak expression of costimulatory molecules ( CD 80 , CD 86 ) and an absence of inflammatory cytokines ( IFNgamma , TNFalpha , IP 10 ) and lymphocyte activation markers ( CD 25 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , these DCs expressed high levels of CD 40 , CD 80 , CD 86 , and MHC class 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
ICOS binds to a specific ligand on antigen presenting cells ( APC ) and cells of the peripheral tissue different from the CD28 / CD152 ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Most of the DCs express immunocytochemically detectable antigens like : S 100 , CD1a , CD 40 receptor , adhesion molecules ( ICAM 1 or CD 54 , LFA 1 and LFA 3 ) , integrins ( CD11a , CD11c and CD 18 ) , CD 45 , CD 54 , co stimulatory molecules ( B 7 1 or CD 80 , B 7 2 or CD 86 ) , F 418 , MHC class 1 and 2 and DEC 205 , multilectin receptor , immunostimulatory cytokine ( IL 12 ) and , of course , Fc and complement receptors . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Nave CD 4 ( + ) T cells require two signals for proper activation and induction of differentiation : signal 1 is provided by peptide antigens in the context of the major histocompatibility complex ( MHC ) class 2 , while signal 2 is delivered by costimulatory molecules such as CD 80 or CD 86 present on the antigen presenting cell ( APC ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using splenic T cells and IPE from donor mice with disrupted genes for CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , CTLA 4 , and / or CD 28 , we report that B 7 2 ( + ) IPE in the presence of anti CD 3 supported selectively the activation of CTLA 4 ( + ) CD 8 ( + ) T cells that express their own B 7 2 and secrete enhanced amounts of active TGFbeta . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immature dendritic cells with characteristic phenotypic markers CD 40 , CD 80 , and CD 86 were successfully isolated from the blood of patients with pancreatic cancer . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This may be due to the direct effect of decreased Tax expression , because the Tax siRNA did not alter the expression of MHC 1 , CD 80 , or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : The expression of CD 83 , CD 80 , and CD 86 on the surface of dendritic cells cultured from the peripheral blood of patients was detected compared with that from health donors using flow cytometry . ^^^ The expression of CD 80 , CD 86 , CD 83 , and S 100 proteins was assessed in esophageal carcinoma tissues using immunohistochemical method . ^^^ RESULTS : Compared with those from healthy donors , dendritic cells cultured from the peripheral blood of patients expressed lower CD 80 and CD 86 . ^^^ Compared with the control group , the positive expression ratio and frequencies of CD 80 , CD 86 , and S 100 in esophageal carcinoma tissues were significantly down regulated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After up regulation of the C3aR by IFN alpha+ IFN gamma , C3a significantly up regulated the surface expression of CD 54 , CD 83 and CD 86 , but not of CD 40 , CD 80 or human leucocyte antigen ( HLA ) DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Markers of DC activation CD 80 and CD 86 were absent from both groups of specimens . ^^^ Flow cytometry analysis of the effects of C . pneumoniae infection on immature monocyte derived DCs in vitro showed no changes in the expression of CD1a , MHC class 2 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of CD 80 , CD 83 , CD 86 , CD1a , and CD 40 on leukemic DCs was significantly lower than that on donor DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules ( CD 80 and CD 86 ) , not associated constitutively with rafts , were expressed at much lower level in A 20 cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The resulting adenovirus transduced DCs differentiated in the presence of monocyte conditioned medium and poly [ 1 ] : poly [ C ] , expressing the surface markers of mature DCs , including CD 83 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Analysis of DC from Flt 3L treated mice revealed an immature phenotype with low or absent expression levels of CD 80 , CD 86 and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present study , blasts from 21 patients with AML were cultured in vitro for 2 days , then cells were gamma irradiated and antigen presenting cell ( APC ) characteristics were assessed . gamma Irradiation induced expression of several characteristics of APCs in AML blasts , including expression of CD 80 , CD 86 , and BDCA 4 , and were stimulators of allogeneic mixed lymphocyte reactions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RTDCs isolated by enzyme digestion , Percoll density gradient centrifugation and overnight GM CSF culture followed by MACS separation retain an archetypical immature dendritic cell phenotype , characterised by MHCII ( low ) CD 40 ( neg ) CD 86 ( neg ) CD 80 ( neg ) CD11c ( low ) cell surface expression . ^^^ Splenic derived DC ( SDC ) isolated conformed to a day 1 in vitro phenotype ; MHCII ( low ) CD 40 ( neg ) CD 86 ( low ) CD 80 ( neg ) CD11c ( low ) but can further mature phenotypically in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , we collected Mo DCs from the collagen gels by collagenase treatment and analyzed the expression of antigen presentation related molecules such as HLA DR , CD 80 , CD 83 , and CD 86 on Mo DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The ingestion of MS did not change the cell surface expression of CD 80 , CD 83 , CD 86 and HLA DR of immature and mature DC , suggesting that MS uptake did not induce DC maturation but that maturation by cytokines or LPS was unaltered in the presence of MS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These activated platelets induced DC maturation , as revealed by significant up regulation of CD 83 , CD 80 , and CD 86 Ags . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study we demonstrate the induction of anergic regulatory T cells during an MLR performed in the presence of blocking mAb to the costimulatory molecules CD 40 , CD 80 , and CD 86 . ^^^ Thus , a subtype of regulatory T cells in human blood can be activated in the absence of costimulatory signals from CD 40 , CD 80 , and CD 86 , and they can be identified by expression of ICOS after activation . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the spleen , Ag bearing cells were CD 1 ( + ) , CD11b ( + ) , ED 1 ( + ) , ED 2 ( low ) , ED 3 ( + ) , CD 86 ( low ) , OX 6 ( + ) , CD11c ( ) , ED 5 ( ) and in the LNs were CD 4 ( + ) , CD 8 ( + ) , CD 80 ( + ) , and OX 41 ( + ) suggesting these were lymphoid organ resident macrophages . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 antigen expression was upregulated on B cells but not T cells during incubation with trimeric human CD 40 ligand ( huCD40LT ) + IL 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In these studies , we demonstrate that exogenous soluble LIGHT or soluble CD 40 ligand ( CD40L ) can promote monocyte derived dendritic cell maturation in vitro by the up regulation of CD 86 , CD 80 , CD 83 , and HLA DR antigen expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Adenovirus serotype 3 utilizes CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) as cellular attachment receptors . ^^^ Herein we present data indicating that adenovirus ( Ad ) serotype 3 utilizes CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) as cellular attachment receptors . ^^^ CD 80 and CD 86 are co stimulatory molecules that are present on mature dendritic cells and B lymphocytes and are involved in stimulating T lymphocyte activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show that isolated NSCs from adult brain express CD 80 and CD 86 ( B 7 2 ) and this expression is increased after exposure to IFN gamma or TNF alpha , the prototypical Th 1 cytokines expressed during EAE . ^^^ CD 80 and CD 86 expressed by NSCs are functional and can costimulate allogeneic cells in a mixed lymphocyte reaction . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
LPS inhibited APC maturation assessed by CD 80 and CD 86 expression , and inhibited chemokine ( CCL 3 and CCL 5 ) production . ^^^ LPS on CD 80 , CD 83 , and CD 86 , and on CCL 3 and CCL 5 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD1a , HLA DR and CD 54 were highly expressed on DC stimulated with both kinds of LPS ; however , CD 40 , CD 80 , CD 83 and CD 86 expression on Pg LPS stimulated DC was lower than on Ec LPS stimulated DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , these cells fail to express conventional costimulatory molecules ( CD 80 , CD 86 ) , leading to the possibility that antigen presented by normal IECs could result in anergy . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
When macrophages were primed with IFN gamma , resveratrol suppressed the expression of HLA ABC , HLA DR , CD 80 , CD 86 and inhibited production of TNF alpha , IL 12 , IL 6 and IL 1beta induced by LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Co stimulatory molecules B 7 1 ( CD 80 ) / B7 2 ( CD 86 ) and their counter receptors CD28 / CTLA 4 play pivotal roles in T cell activation and immune regulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this syndrome , CD154 / CD40 interactions are required for but do not mediate disease by upregulation of CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
But CD 80 and CD 86 were expressed at low levels or absent on IL 3 treated DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The GPA ( ) cells were comprised of cells with excentric cell shape and were positive for HLA class 1 , HLA class 2 , CD1a , CD 4 , CD11c , CD 14 , CD 40 , CD 80 , CD 83 , and CD 86 , but not for CD 3 , CD 8 , CD 19 , CD 20 , and CD 56 , indicating the codevelopment of dendritic cells ( DC ) along with erythroid differentiation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Because freshly prepared spleen B cells are highly sensitive to irradiation , we employed spleen B cells stimulated with lipopolysaccharide ( LPS B cells ) . 10 irradiation ( 8 Gy ) followed by incubation ( 9 12 hr ) highly and selectively up regulated CD 80 expression in LPS B cells , whereas the same treatment slightly increased expression of CD 54 and did not affect expression of CD 86 , major histocompatibility complex class 2 , CD11a or surface immunoglobulin M . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Co treatment of PA with LPS further increased dendritic cell surface marker expressions ( CD 80 , CD 86 , CD 40 , MHC class 1 , and class 2 antigens ) and reduced the phagocytic activity of LPS treated cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
HCs upregulate surface expression of major histocompatibility complex ( MHC ) class 1 molecules and CD1d but not MHC class 2 molecules Qa 1 , CD 80 , CD 86 , CD 54 , or CD 95 ; in addition , they expressed / secreted interleukin ( IL ) 10 and IL 4 but not IL 1 , IL 6 , IL 13 , interferon ( IFN ) gamma , tumor necrosis factor ( TNF ) , IL 4 , or IL 27 ( i . e . , they acquire the HC * phenotype ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The most widely comprehended costimulatory pathway involves the ligation of CD 28 expressed on T cells by CD 80 and CD 86 upregulated on APCs . ^^^ We found that regardless of the maturation stage , CD 80 and CD 86 were evident within the plaques , and colocalized with the presence of markers of dendritic cells and with expression of the extensively investigated autoantigen oxidized LDL . ^^^ FACS analysis studies showed that splenocytes from aged atherosclerotic apoE KO mice exhibited increased expression on B cells ( which represent APCs ) of CD 80 and CD 86 as compared to their young apoE or nave C57BL / 6 litters that have no plaques . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The B 7 ligands CD 80 and CD 86 on APCs deliver either costimulatory or inhibitory signals to the T cell when interacting with their counter receptors CD 28 and CD 152 ( CTLA 4 ) on the T cell surface . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The results showed that DCs cultured in the presence of MPA expressed low levels of CD 40 , CD 80 and CD 86 , and exhibited weak activity in stimulating the proliferation of allogeneic T cells and antigen presenting function with a concurrent reduction of IL 12 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Chronic ethanol ingestion by mice increases expression of CD 80 and CD 86 by activated macrophages . ^^^ We now report that in vitro activated CD11b ( + ) splenocytes obtained from C57BL / 6 and BALB / c mice that consumed ethanol chronically express increased levels of the T cell co stimulatory molecules CD 80 and CD 86 . ^^^ Evaluation of cultures of purified CD11b ( + ) cells , obtained from mice that consumed ethanol chronically , incubated overnight , showed increased up regulation of CD 80 and CD 86 expression on Gr . 1 ( ) mouse splenic macrophages . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Insertion of host derived costimulatory molecules CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) into human immunodeficiency virus type 1 affects the virus life cycle . ^^^ Two major cell types that harbor HIV 1 in vivo , i . e . , monocytes / macrophages and CD4+ T cells , express the costimulatory molecules CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) . ^^^ We investigated whether CD 80 and CD 86 are efficiently acquired by HIV 1 , and if so , whether these host encoded molecules can contribute to the virus life cycle . ^^^ Here we provide the first evidence that the insertion of CD 80 and CD 86 into HIV 1 increases virus infectivity by facilitating the attachment and entry process due to interactions with their two natural ligands , CD 28 and CTLA 4 . ^^^ Moreover , we demonstrate that NF kappaB is induced by CD 80 and CD 86 bearing virions when they are combined with the engagement of the T cell receptor / CD3 complex , an event that is inhibited upon surface expression of CTLA 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recently we reported that high potency ( CMRF 44 ( + ) ) dendritic cells ( DC ) in the peripheral blood of patients with MM failed to significantly up regulate the expression of the B 7 co stimulatory molecules , CD 80 and CD 86 , in response to an appropriate signal from soluble trimeric human CD 40 ligand . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Monocyte staining showed significant increases in human leukocyte antigen D related , CD 54 , CD 80 , and CD 86 surface markers in intervillous blood , compared with peripheral blood , which suggests a relative activation of monocytes in the placenta . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vivo experiments in the mouse DA 1 3b / C3H whole animal acute myeloid leukemia ( AML ) model showed that injection of Ara C induced expression of CD 80 and CD 86 , and decreased expression of B 7 H1 , indicating that chemotherapy can modify costimulatory molecule expression in vivo , in a way not necessarily observed in vitro . ^^^ Ara C also induced CD 80 or CD 86 expression in 14 of 21 primary cultured human AML samples . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Memory B cells in SSc showed increased expression of activation markers , including CD 80 , CD 86 , and CD 95 , relative to normal controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 10 modified DC resulted in lower clinical scores , less body weight loss , lower numbers of anti AChR IgG antibody secreting cells and lower affinity of anti AChR antibodies in rats receiving IL 10 modified DC , accompanied with lower expression of CD 80 and CD 86 and lower lymphocyte proliferation among lymph node mononuclear cells compared with control EAMG rats . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We also observed that at the molecular level Tomatine required both CD 80 and CD 86 costimulation to engender antigen specific cellular immunity . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The role of B 7 family members CD 80 and CD 86 in providing costimulatory signals to T cells is well established . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition to cytokine induction , TLR activation by fimbriae also results in upregulation of the CD 40 , CD 80 , and CD 86 costimulatory molecules in antigen presenting cells , suggesting that fimbriae are sensed as a potential `` danger ' ' to the host immune system . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Generation of tolerogenic dendritic cells by treatment with mitomycin C : inhibition of allogeneic T cell response is mediated by downregulation of ICAM 1 , CD 80 , and CD 86 . ^^^ MMC treatment selectively decreases adhesion ( intercellular adhesion molecule [ ICAM ] 1 ) and co stimulatory ( CD 80 , CD 86 ) molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometry showed that day 5 dendritic cells ( DC ) were less mature than day 7 DC as indicated by the expression of CD1a , CD11c , CD 14 , CD 80 , CD 83 , CD 86 and MHC 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recombinant ( r ) PorA induced mo DC maturation , as reflected by reduced receptor mediated endocytosis , increased production of the chemokines IL 8 , RANTES , MIP 1 alpha and MIP 1 beta and augmented expression of the surface markers CD 40 , CD 54 , CD 80 , CD 86 and major histocompatibility complex class 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results show that MSCs inhibit the up regulation of CD1a , CD 40 , CD 80 , CD 86 , and HLA DR during DC differentiation and prevent an increase of CD 40 , CD 86 , and CD 83 expression during DC maturation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD11c ( approximately 90 % ) , MHC 2 ( approximately 70 % ) , CD 86 ( approximately 20 % ) , CD 83 ( approximately 5 % ) , CD 80 ( approximately 40 % ) , CD 40 ( approximately 40 % ) , and CCR 7 ( approximately 5 % ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
By a direct flow cytometry approach , the blood levels of circulating lineage ( CD 3 , CD 19 , CD 14 ) negative , CD33++ , HLA DR+ cells were estimated before and following ex vivo cell differentiation , and phenotyped by MAbs with specificity against HLA DR , HLA ABC , CD1a , CD11c , CD 33 , CD 40 , CD49d , CD49e , CD 54 , CD 80 , CD 83 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The performance of DCs in mixed lymphocyte reaction correlated significantly ( p = 0 . 005 ) with the CD 83 expression but not with the CD 80 , CD 86 , HLA DR , CD1a , and CD1c expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , when HL 60 cells were treated with A 23187 for 96 hours , the expressions of CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , MHC class 2 molecule and CD 54 on HL 60 cells reached peak , and marked activation of allogeneic T cells occurred . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Importantly , activation of CTL by OVA CT pulsed DC was dependent on CT induced activation of adenylate cyclase and increased cAMP production by DC associated with increased expression of MHC class 1 and co stimulatory molecules ( CD 80 , CD 86 and CD 40 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Liver sinusoidal endothelial cells ( LSEC ) have been reported to express MHC class 2 , CD 80 , CD 86 , and CD11c and effectively stimulate naive T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Monocytes exposed to EBV became larger in size with increased granularity and expressed considerably higher levels of membrane HLA classes 1 and 2 , ICAM 1 , CD 80 , CD 86 , and CD 40 compared with uninfected cultures . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The gammadelta T cell effect upon the LNC response to MBP did not involve a change in expression of the costimulatory molecules CD 28 , CD40L , and CTLA 4 on TCRalphabeta ( + ) cells , and CD 40 , CD 80 , and CD 86 on CD 19 ( + ) and CD11b ( + ) cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
HSP60 priming of monocytes also results in significant down regulation of HLA DR , CD 86 and Toll like receptor 4 expression , but minimally up regulates CD 80 expression , similar to that previously reported with LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
LPS stimulation resulted in a dose dependent up regulation of CD 40 , CD 80 , and CD 86 on monocytes , and up regulation of CD 69 on NK cells and gamma delta T cells in both the periodontitis and non periodontitis groups . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The SR A contributes to phagocytosis of the organisms but not to their ability to induce CD 80 , CD 86 , and MARCO or to inhibit MHC 2 . ^^^ Conversely , studies with lipopolysaccharide ( LPS ) deficient organisms and / or TLR 4 mutant mice showed that LPS and TLR 4 are at least partially required to induce CD 80 , CD 86 , and MARCO , but LPS is not required to inhibit MHC 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expressions of CD 80 , CD 86 and HLA DR on DC were slightly increased . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Lower percentages of monocytes with CD 80 , CD 86 and HLA DR molecule expression in pediatric cancer . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Transfection of IFN gamma gene into DC promoted DC to express higher CD 40 , CD 54 , CD 80 , CD 86 , CCR 7 and 1 Ab , secrete more IL 1beta and IL 12p70 , and more potently activate both CD 4 and CD 8 T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Tacrolimus and steroid ointment induced a selective depletion of IDECs from the epidermis and reduced the expression of the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The effect of TNF alpha neutralisation on the phenotype ( CD 14 , CD 16 , CD 32 , CD 64 , CD 80 , CD 83 , CD 86 , and MHC ) of dendritic cells was investigated by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Dendrimer glucosamine inhibited Toll like receptor 4 mediated lipopolysaccharide induced synthesis of pro inflammatory chemokines ( MIP 1 alpha , MIP 1 beta , IL 8 ) and cytokines ( TNF alpha , IL 1 beta , IL 6 ) from human dendritic cells and macrophages but allowed upregulation of the costimulatory molecules CD 25 , CD 80 , CD 83 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 27 ( + ) IgD ( ) memory B cells , i . e . , cells with hyper mutated IgV genes , were significantly increased in the CSF vs . paired peripheral blood and displayed up regulation of the CD 80 and CD 86 costimulatory molecules and of CC chemokine receptor ( CCR ) 1 , CCR 2 , and CCR 4 in both patient groups . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting analysis of cell surface expression of maturation marker molecules such as CD 80 , CD 83 , CD 86 , and HLA DR revealed equal upregulation after stimulation with H . pylori or LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NF kappaB binding activities , costimulatory molecule ( CD 40 , CD 80 , CD 86 ) surface expression , IL 12 protein expression and allostimulatory capacity of DCs were measured with electrophoretic mobility shift assay ( EMSA ) , flow cytometry , Western blotting , and mixed lymphocyte reaction ( MLR ) , respectively . ^^^ RESULTS : GM CSF propagated DCs , GM CSF+NF kappaB decoy ODNs propagated DCs and GM CSF+ scrambled ODNs propagated DCs exhibited features of immature DCs , with similar low level of costimulatory molecule ( CD 40 , CD 80 , CD 86 ) surface expression , absence of NF kappaB activation , and few allocostimulatory activities . ^^^ GM CSF+IL 4 propagated DCs displayed features of mature DCs , with high levels of costimulatory molecule ( CD 40 , CD 80 , CD 86 ) surface expression , marked NF kappaB activation , and significant allocostimulatory activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OK PSA treatment effectively increased the surface expression of MHC class 2 , CD 80 , CD 83 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) were induced by listerial infection only when the bacteria invaded the cytoplasm . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , CTL responses were associated with a partial activation of neonatal CD11c ( + ) DC , reflected by the up regulation of CD 80 and CD 86 expression but no concurrent changes in MHC class 2 or CD 40 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
All mature DCs uniformly expressed comparable levels of HLA DR , CD 83 , CD 80 , and CD 86 , and were potent stimulators of allogeneic T cells after exposure either to recombinant human CD40L trimer or a combination of inflammatory cytokines with PGE ( 2 ) . moDCs , however , required 0 . 5 1 log greater numbers than LCs or DDC IDCs to stimulate comparable T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : DCs up regulated the expression of CD1a ( 55 . 0 % ) , CD11c ( 98 . 6 % ) , CD 86 ( 86 . 1 % ) , CD 80 ( 66 . 1 % ) , and HLA DR ( 88 . 9 % ) after cultured for 5 days . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , unlike EBOV and MARV , VLPs induced maturation of DCs including upregulation of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) , major histocompatibility complex ( MHC ) class 1 and 2 surface antigens , and the late DC maturation marker CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Following 6 days in culture , four of five cell lines demonstrated dendrite like morphology , upregulation of CCR 7 , CD 54 , CD 80 and CD 86 , uptake of extracellular proteins and activation of T cells , and similar results were obtained with blasts from three t ( 9 ; 22 ) ALL patients . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CpG ODN induced enhancement of CHS was due to increased antigen presenting cell functions of DC since : ( 1 ) CpG ODN injected skin revealed upregulated expression of major histocompatibility complex class 2 , CD 80 , and CD 86 molecules and ( 2 ) CpG ODN treatment of DNFB derivatized DC enhanced the intensity of CHS responses after in vivo transfer . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , a distinct population of CD11c ( + ) MHCII ( high ) DC showing low expression of the costimulatory molecules CD 40 , CD 80 , and CD 86 in wild type animals was virtually absent in skin draining lymph nodes of CCR 7 deficient mice under steady state conditions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that mice nasally administered with Stx 1 B or mStx 1 showed an up regulation in the expression of CD 80 , CD 86 and especially CD 40 on NALT DCs . ^^^ Taken together , these results suggest that non toxic Stx derivatives could be effective mucosal adjuvants for the induction of Th 2 type , CD4+ T cell mediated , antigen specific mucosal IgA and systemic IgG Ab responses , and that they likely owe their adjuvant activity to the up regulation of co stimulatory molecules including CD 80 , CD 86 and CD 40 on NALT DCs . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Comparing the surface induction of CD 83 with the upregulation of CD 40 , CD 80 and CD 86 during LPS ( lipopolysaccharide ) induced DC maturation showed that CD 83 induction occurred more rapidly . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
PS liposomes inhibited the up regulation of HLA ABC , HLA DR , CD 80 , CD 86 , CD 40 , and CD 83 , as well as the production of IL 12p70 by human DCs in response to LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Alcohol treated DCs showed reduced IL 12 , increased IL 10 production , and a decrease in expression of the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of the surface molecules CD 80 , CD 86 , HLA DR etc . on the DC was examined by fluorescent activated cell sorter ( FACS ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 10 induced CD 14 ( + ) CD 16 ( ++ ) cells that expressed dendritic cell markers : CD 80 , CD 86 , HLA DR , and CD 83 and initiated significantly decreased allogeneic mixed lymphocyte reactions ( MLRs ) . ^^^ Blockage of CD 86 , but not CD 80 , further down modulated MLRs induced by CD 14 ( + ) CD 16 ( ++ ) cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , in parallel with their differential expression of costimulatory molecules CD 80 and CD 86 and lack of IL 12 , nicDCs display profoundly reduced Th 1 promoting capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , the expression of maturation markers such as MHC 2 , CD 40 , CD 80 and CD 86 , and of differentiation markers such as CD 4 , CD 8 and CD11b , was also comparable in the two populations . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The adaptive immune response is dependent upon upregulation of costimulatory molecules ( UCM ) such as CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
TgHSP 70 induced the maturation of human monocyte derived dendritic cells as determined by increased levels of surface markers , namely , CD 40 , CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Culture of PBMC with alum induced increased expression of CD 86 ( P=0 . 004 ) and HLA ( P=0 . 01 ) on monocytes while the expression of CD 80 was decreased ( P=0 . 02 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Accordingly , a decreased percentage of mDC expressing CD 80 and CD 86 was observed after maturation , compared to controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
GCP 2 increased CD 80 and CD 86 expression on B cells during in vitro stimulation in a dose dependent manner . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The increase in costimulatory ( CD 80 and CD 86 ) and adhesion ( ICAM 1 , PGP 1 , LFA 1 , and Mac 1 ) in peripheral blood mononuclear cells was also attenuated by food restriction and to a lesser extent by fish oil alone . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mature DC are characterized by active surface expression of MHC and co stimulatory molecules ( MHC 1 , MHC 2 , CD 40 , CD 80 , CD 86 ) . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Compared to immature DC ( imDC ) cultured by GM CSF alone , the TGFbeta DC express lower CD 80 , CD 86 , 1 Ab and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : PBMCs of the healthy donor cocultured with rhGM CSF plus CI for 40 hours had the typical morphology of DCs , with decreased CD 14 expression , and increased CD 83 , CD 80 and CD 86 expressions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , unlike DC , MP were early and strongly activated in response to MV , as shown by the increased surface expression of CD 86 , CD 80 , CD 54 , CD 40 , and HLA abc and by the production of mRNA encoding alpha interferon ( IFN alpha ) , IFN beta , tumor necrosis factor alpha and interleukin 6 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Also , PL enhanced the expression of costimulatory molecules , CD 80 and CD 86 , and major histocompatibility complex ( MHC ) molecules 2 in PM . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Murine bone marrow derived DCs on culture with Bryo 1 alone , Bryo 1 + calcium ionophore ( CI ) , but not CI alone exhibited morphologic changes characteristic of mature DCs and expressed increased levels of CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : Twenty eight HCC samples obtained by surgical resection were analyzed for the expression of beta 2 microglobulin , heat shock protein ( HSP ) 70 , human leukocyte antigen ( HLA ) class 1 , CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) and IL 10 by immunostaining . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that lycopene down regulates the expression of costimulatory molecules ( CD 80 and CD 86 ) and major histocompatibility complex type 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These mPhi showed increased production of interferon gamma ( IFNgamma ) , exclusive production of interleukin 18 ( IL 18 ) and increased expression of major histocompatibility complex ( MHC ) class 2 , CD 40 , CD 80 and CD 86 , when compared with thioglycolate induced mPhi . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of the B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules on the surface of leukemia cells made unnecessary the additional CD 28 costimulation of T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypically `` immature ' ' dendritic cells ( DCs ) , defined by low cell surface CD 40 , CD 80 , and CD 86 can elicit host immune suppression in allotransplantation and autoimmunity . ^^^ CD 40 , CD 80 , and CD 86 cell surface molecules were specifically down regulated by treating NOD DCs ex vivo with a mixture of antisense oligonucleotides targeting the CD 40 , CD 80 , and CD 86 primary transcripts . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Finally , we show that donor corneal APC demonstrate high expression of CD 40 , CD 80 , and CD 86 costimulatory molecules when derived from HR but not LR grafts . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NB 4 cells up regulated markers found in DCs , including HLA DR , costimulatory molecules ( CD 80 and CD 86 ) , adhesion molecules ( CD 40 ) , and chemokine receptors ( CCR 6 ) when cultured for 8 days in the presence of 1 microM ATRA . ^^^ CD11b was coexpressed with CD 80 , CD 83 , and CD 86 in ATRA treated NB 4 cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , the costimulatory signal of CD 86 was selectively blocked , and that of CD 80 was kept unimpaired by administration of anti murine CD 86 monoclonal antibody at the early gestational stage in abortion prone CBA / JxDBA / 2 matings and normal pregnant CBA / JxBALB / c matings . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results show that LTA and MA regulate expression of CD 86 on macrophages while the expression of CD 80 remains unmodified . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Estrogen did not affect the expression of MHC class 2 , CD 80 and CD 86 by DC , but inhibited the ability of DC to stimulate T cell proliferation and production of both Th 1 and Th 2 cytokines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NES , but not heat inactivated NES , up regulates DC maturation markers associated with Th 2 promotion ( CD 86 and OX40L ) , with little change to CD 80 and MHC class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
PNIPAAm g PP membranes containing adsorbed anti mouse CD 80 monoclonal antibody preferentially captured mouse CD 80 transfected cells at 37 degrees C compared with membranes lacking antibody or containing anti mouse CD 86 monoclonal antibody . ^^^ With this method , mouse CD 80 or mouse CD 86 transfected cells were enriched from a 1 : 1 cell suspension to 72 % or 66 % , simply and with high yield . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
SP 600125 , a specific inhibitor of JNK , inhibited the LPS induced up regulation of CD 80 , CD 83 , CD 86 and CD 54 , but augmented the up regulation of HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This FcRH 1 down regulation coincides with dramatic enlargement of the pre germinal center cells , cell cycle entry , and other overt signs of activation that include CD 80 and CD 86 up regulation and immunoglobulin D ( IgD ) down regulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In comparison to immature Mo DC that were derived by culture with GM CSF and IL 13 only , CD 40 , CD 80 , CD 86 , HLA ABC and HLA DR molecules were up regulated upon OM 197 or LPS treatment similarly . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 expression got enhanced on the B 1a cells whereas CD 86 got solely expressed on B 1b cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BCG treated DCs enhanced significantly the expressions of CD 80 , CD 86 , and CD 40 , and the productions of IL 12 and IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The AML cells showed clear upregulation of CD 80 , CD 83 , CD 86 , CD 40 , and HLA DR expression under all culture conditions , without significant differences between these groups . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A number of receptor / ligand pairs have been shown to mediate costimulation , including CD28 / B7 molecules ( CD 80 and CD 86 ) , CD40 / CD40 ligand ( CD40L , CD 154 ) , and LFA 1 ( CD 18 ) / ICAM 1 ( CD 54 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , we demonstrated that our monoclonal antibodies , such as 9H10 and 4C12 , could trigger OX40L reverse signal that enhanced IgG production of B cells and promoted maturation of DCs as evidenced by the upexpression of CD 80 , CD 86 , CD 83 and CXCR 4 and monoclonal antibody 9H10 could also promote anti CD 40 monoclonal antibody stimulated DCs in order to induce T cells to secrete more interleukin 2 and interferon gamma , which suggested that OX40L signals could strengthen the effect of CD 40 signals on promoting Th 1 differentiation . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 28 induces immunostimulatory signals in dendritic cells via CD 80 and CD 86 . ^^^ Production of interleukin 6 required B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) and p 38 mitogen activated protein kinase and prevented interferon gamma driven expression of immunosuppressive tryptophan catabolism . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Before culture , cells did not express CD1a , CD 80 and CD 83 , while expressed CD 54 , CD 86 and HLA DR with low frequency . ^^^ After culture , cells upregulated CD1a , CD 80 , CD 83 , CD 54 , CD 86 and HLA DR significantly . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The ratio of CD8+ DC to CD 8 DC increased , and PG enhanced IL 12 and IFN gamma production , and expression of surface molecules including major histocompatibility complexes ( MHC ) classes 1 , MHC 2 , CD 80 , and CD 86 in MCA 102 challenged mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of CD 80 and CD 86 was enhanced by IFNgamma treatment but was not modified by IL 1beta ; the expression of CD 86 and HLA DR was reduced by TGFbeta 1 treatment . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The aim of the study was to estimate the expression of CTLA 4 ( cytolitic T lymphocyte associated antigen 4 , CD 152 ) , CD 28 , B7 . 1 ( CD 80 ) and CD7 . 2 ( CD 86 ) molecules on peripheral blood cells in patients with Graves ' disease ( GD ) ( n=28 , mean age 15 . 4 ) , in patients with nontoxic nodular goiter ( NTNG ) ( n=28 , mean age 15 . 6 years ) in comparison with sex and age matched healthy control subjects ( n=28 , mean age 15 . 9 years ) . ^^^ The analysis of CD3+ T lymphocytes co expressing CD 152 and CD 28 antigens on peripheral blood revealed increased percentages of CTLA 4 / CD28 positive cells in patients with Graves ' disease ( p < 0 . 004 , p < 0 . 04 ) compared to the controls and euthyroid Graves ' patients , while B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) molecules were detected only in some hyperthyroid patients on activated monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 86 expression was significantly enhanced ( P < 0 . 05 ) by stimulation with either CD40L AP ( 75 . 5+ / 14 . 5 % ) or sCD40L ( 80 . 5 % + / 5 . 3 % ) compared with immature DC ( 55 . 2+ / 14 . 8 % ) , as were CD 80 and CD 83 . ^^^ Similarly , in large scale studies using Isolex 300I to enrich for monocytes and platelets for DC generation / maturation on a clinical scale , stimulation with CD40L AP increased CD 86 and CD 80 expression as well as the ability to stimulate allogeneic lymphocytes compared with control cultures . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Lentiviral transduction of DCs resulted in down regulation of cell surface molecules including CD1a , co stimulatory molecules CD 80 , CD 86 , ICAM 1 , and DC SIGN . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Incubation of immature human PBMC derived DCs with SHIV VLPs for 48 h resulted in the significant up regulation of CD 40 , CD 80 , CD 83 , CD 54 , CD 86 , HLA A , B , C and HLA DR , DP , DQ molecules on activated DC CD11c+ subpopulations . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , the expression of HLA DR , CD 80 and CD 86 , was significantly reduced on rhIL 10 treated dendritic cell subsets . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The FastDCs and novel dendritomas were immunostained with anti CD 80 , anti CD 86 , anti CD 83 , anti HLA DR mAbs and analyzed by fluorescence activated cell sorting ( FACS ) . ^^^ RESULTS : Mature FastDCs with highly expressed surface markers CD 80 , CD 86 , CD 83 and HLA DR were generated within 48 h in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 , but not CD 154 , augment DNA vaccine induced protection in experimental bovine tuberculosis . ^^^ The co administration of costimulatory molecules CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) and CD 154 ( CD40L ) has been shown to enhance immune responses in several murine models . ^^^ In these studies , we demonstrate that the co administration of CD 80 and CD 86 , but not CD 154 , to an existing candidate subunit DNA vaccine ( ESAT 6 ) against bovine tuberculosis , enhances protection after aerosol challenge with virulent Mycobacterium bovis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast to LMP 1 , LMP1CD40 caused an upregulation of CD 69 , CD 80 , and CD 86 in B cells and a dramatic increase in serum immunoglobulin M . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interferon beta up regulates the expression of co stimulatory molecules CD 80 , CD 86 and CD 40 on monocytes : significance for treatment of multiple sclerosis . ^^^ Peripheral blood mononuclear cells ( PBMCs ) were stimulated with IFN beta in vitro and expression of CD 80 , CD 86 , CD 40 and HLA was examined by flow cytometry and reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Concomitantly with HTLV 1 expression , these ATL cells expressed co stimulatory molecules such as CD 80 , CD 86 and OX 40 , and showed elevated levels of antigenicity against allogeneic T cells and HTLV 1 Tax specific cytotoxic T lymphocytes ( CTL ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the in vitro experiments , OK PSA enhanced expression of MHC class 2 , CD 80 and CD 86 , as well as IL 12 production on dendritic cells ( DCs ) were derived from wild type mice , but not from TLR 4 deficient ( TLR 4 / ) mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expressions of CD 80 , CD 86 were lower on TGFbeta DC than on imDC [ ( 4 . 14 + / 0 . 95 ) % vs ( 13 . 90 + / 7 . 22 ) % ; ( 8 . 60 + / 0 . 75 ) % vs ( 20 . 63 + / 5 . 03 ) % , P < 0 . 05 , both ] . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Infection of DC with Y . enterocolitica wild type resulted in decreased up regulation of major histocompatibility complex class 2 , CD 54 ( intercellular adhesion molecule 1 ) , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The inactivation of the MAPK pathway in both macrophages and dendritic cells leads to inhibition of proinflammatory cytokine secretion , downregulation of costimulatory molecules such as CD 80 and CD 86 , and ineffective T cell priming . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We were able to demonstrate that AAV / BA46 / Neo loading of DCs resulted in : ( 1 ) BA 46 expression in DCs , ( 2 ) chromosomal integration of the AAV / BA46 / Neo vector within DCs , ( 3 ) strong , rapid BA 46 specific , MHC class 1 restricted CTLs in only 1 week , ( 4 ) T cell populations with significant interferon gamma ( IFN gamma ) expression but low IL 4 expression , ( 5 ) high CD 80 and CD 86 expression in DCs , and ( 6 ) high CD 8 : CD 4 and CD 8 : CD 56 T cell ratios . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 induction by IL 2 were positively regulated through the mitogen activated protein kinase pathway , while CD 86 expression was negatively regulated through Janus kinase pathways . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Fluorescence activated cell sorting revealed that XS 106 IDO DCs were not changeable for Ia , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These DC s express MHC 2 , the costimulatory molecules CD 40 , CD 80 , CD 86 , adhesion molecules such as DC SIGN and chemokine receptors such as CCR 7 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
MSC were negative for MHC 2 , CD 40 , CD40L , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , positive for MHC 1 , and kept this expression pattern during osteogenic differentiation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Disease improvement was paralleled by decreased expression of MHC class 2 on monocytes and B lymphocytes and reduced expression of CD 80 and CD 86 on B lymphocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In both cases , infiltration by APCs was accompanied by strong CD 80 and CD 86 expression and macrophage nitric oxide ( NO ) synthase up regulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Costimulatory molecules such as lymphocyte function associated antigen ( LFA ) 1 ( CD11a ) , LFA 3 ( CD 58 ) , intercellular adhesion molecule ( ICAM ) 1 ( CD 54 ) , neuronal cell adhesion molecule ( NCAM ) ( CD 56 ) , B 7 1 ( CD 80 ) , or B 7 2 ( CD 86 ) are important regulatory elements in healthy immunological cascades , but their role in acute myeloid leukemia ( AML ) has only been rarely investigated . ^^^ The surface expression of CD11a ( 27 of 29 cases positive with an average of 71 % positive blasts ; 27 ( + ) / 29 , 71 % ) , CD 54 ( 23 ( + ) / 33 , 37 % ) , CD 56 ( 24 ( + ) / 93 , 20 % ) , CD 58 ( 29 ( + ) / 29 , 95 % ) , CD 80 ( 13 ( + ) / 28 , 30 % ) , and CD 86 ( 19 ( + ) / 29 , 39 % ) was measured . ^^^ CD 80 and CD 86 expressions were similar in all FAB types . ^^^ Patients who had responded to AML CG therapy showed higher CD 80 proportions and lower CD 86 proportions compared to the `` nonresponder ' ' group . ^^^ Whereas cases with more than 15 % CD 80 ( + ) cells had a significantly lower probability for relapse free survival , only cases with more than 65 % CD 86 ( + ) were characterized by a significantly lower probability for relapse free survival . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
There are also features of activation observed in the splenic macrophages of mice consuming ethanol chronically , including increased up regulation of CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Activation also increased the proportion of OX62+ cells expressing CD 40 , CD 54 and CD 86 and their intensity of expression , however , unlike murine BMDCs , it had no effect on CD 80 and MHC class 2 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 40 , CD 80 and CD 86 ) at the T cell DC synapse . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
As an extracellular delivered protein , Vpr down modulated the expression of several immunologically important molecules including CD 40 , CD 80 , CD 83 and CD 86 costimulatory molecules on MDM ( monocyte derived macrophage ) and MDDC ( monocyte derived dendritic cells ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The aim of the study was to evaluate the function of monocytes in children with leukemias and lymphomas based on the expression of critical costimulatory , activatory and adhesion molecules ( CD 80 , CD 86 , HLA DR and CD 54 = ICAM 1 ) , estimated with tricolor flow cytometry . ^^^ In comparison to the control group we found a lower percentage of monocytes with costimulatory molecules ( CD 80 before and CD 86 after lipopolysaccharide stimulation ) at the time of diagnosis and of monocytes with HLA DR molecules after remission induction . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of HLA ABC , HLA DR , CD 40 , CD 80 , CD 83 and CD 86 was evaluated by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The role of costimulatory molecules CD 80 and CD 86 and IFNgamma in the pathogenesis of ulcerative colitis . ^^^ We quantified the expression of CD 80 , CD 86 , and IFNgamma in colonic mucosa of patients affected by ulcerative colitis and correlated it with clinical and biochemical parameters to identify the context of this up regulation . ^^^ CD 80 , CD 86 , and IFNgamma expression in the colonic mucosa was quantified using reverse transcription polymerase chain reaction . ^^^ CD 80 was detectable in seven patients , while CD 86 and IFNgamma expression was evident in all UC patients . ^^^ CD 80 and CD 86 were not detectable in control specimens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulated B cells ( sBc ) express high levels of MHC class 1 and 2 , as well as the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Currently , numerous preclinical studies have reported immunomodulatory cytokine gene therapy , such as interleukin 2 , interleukin 12 , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) and granulocyte macrophage colony stimulating factor . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Consistent with the receptor expression , AMs showed no sensitivity to CpG ODN , whereas other macrophage populations secreted tumor necrosis factor alpha , interleukin 12 p 40 , and interleukin 6 , and enhanced expression of CD 40 , CD 80 , and CD 86 , in response to CpG ODN . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , levels of CD 80 and CD 86 on human monocytic THP 1 cells were decreased in a dose dependent manner in the presence of 0 . 1 to 10 microM DZ 2002 , and decreases were also seen in IL 12 and tumor necrosis factor alpha production from both mouse thioglycollate stimulated peritoneal macrophages and THP 1 cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS : Airway EOSs were recovered from ovalbumin sensitized and challenged BALB / c mice , these EOSs were then cocultured with CD4+ cells isolated from sensitized mice in the absence or presence of anti CD 80 or / and CD 86 monoclonal antibodies . ^^^ RESULTS : Airway EOSs functioned as CD 80 and CD 86 dependent antigen presenting cells to stimulate lung CD4+ lymphocytes to produce interleukin 4 , interleukin 5 and interleukin 13 , but not interferon gamma in in vitro assay . ^^^ When instilled intratracheally in sensitized recipient mice , airway EOSs primed lung Th 2 cells in vivo for interleukin 4 , interleukin 5 and interleukin 13 , but not interferon gamma , production during the in vitro culture that was also CD 80 and CD 86 dependent . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast to both nonleukemic B cell precursors and mature B cells , the response of precursor B ALL cells was characterized by increased CD 40 expression but only small changes in CD 86 levels and no induction of CD 80 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The percentages of blood DC expressing the cell surface marker CD1a , co stimulatory molecules CD 80 and CD 86 , adhesion molecule CD 54 , and chemokine receptors CCR 1 , CCR 2 , CCR 5 , and CXCR 4 were not affected in GBS or CIDP . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Exposure to proteoliposomes from serogroup B Neisseria meningitidis ( PL ) induced up regulation of MHC 2 , MHC 1 , CD 40 , CD 80 and CD 86 expression on the surface of murine bone marrow derived dendritic cells ( DC ) . ^^^ CD 40 , CD 80 and CD 86 were up regulated on bone marrow derived macrophages ( MPhi ) upon stimulation with PL . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using one CpG ODN , DSP 30 , we observed that it could upregulate not only Toll like receptor 9 ( TLR 9 ) mRNA expression in activated B cells , but also the early expression of CD 69 followed by the sequential expression of CD 80 , CD 86 and the nuclear factor ( NF ) kappaB pathway . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
While no diurnal rhythmicity in the expression of TLRs 1 , 2 , 4 or 9 was observed , the upregulation of costimulatory ( CD 80 and CD 86 ) and antigen presenting ( MHC class 2 ) molecules on CD 14 ( + ) monocytes following activation with specific TLR ligands was greater ( P < 0 . 05 ) in samples obtained in the evening compared with the morning . ^^^ Furthermore , the upregulation of CD 80 , CD 86 , MHC class 2 and interleukin 6 by CD 14 ( + ) monocytes following activation with specific TLR ligands was decreased ( P < 0 . 05 ) in samples obtained following exercise compared with at rest . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although CIITA ( / ) DC lack MHC class 2 , they maintained normal expression of costimulatory molecules CD 80 , CD 86 , and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Sections were stained with antibodies against : EG 2 ( eosinophils ) , CD 4 ( T cells ) , CD 68 ( macrophages ) , CD1a ( Langerhans cells ) , CD 28 ( on T cells ) and costimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Triptolide suppresses CD 80 and CD 86 expressions and IL 12 production in THP 1 cells . ^^^ RESULTS : Triptolide suppressed CD 80 and CD 86 expressions on IFN gamma ( 500 kU / L ) and LPS ( 1 mg / L ) activated THP 1 cells at nontoxic dosages of 2 . 5 0 . 625 microg / L . ^^^ CONCLUSION : Triptolide impairs the antigen presenting function by inhibiting CD 80 and CD 86 expressions and decreased IL 12p40 and IL 12p70 ( bioactive form ) productions from the activated THP 1 cells . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This study investigated how CD 80 and CD 86 regulated CD 152 expression in a low responding cardiac transplant model with CD 152 mediated long term graft acceptance . ^^^ Proliferation assays , quantitative ( Q ) real time polymerase chain reaction ( PCR ) , flow cytometric analyses , and fluorescence microscopy were conducted to examine the roles of CD 80 and CD 86 in CD 152 expression . ^^^ A 100 splenic T cells were hyporesponsive to donor specific stimulation , and anti CD 80 , anti CD 86 , or anti CD 152 treatment significantly enhanced the proliferation response of the B10 . ^^^ Proliferation assays and Q PCR revealed that CD 152 inhibited T cell proliferation and , at the same time , decreased CD 152 expression by secluding CD 80 and CD 86 from CD 28 engagement . ^^^ Besides , CD 152 engagement by CD 80 decreased CD 152 mRNA transcription , and CD 152 engagement by CD 86 inhibited surface expression of CD 152 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Addition of JM 34 during DC maturation inhibited the expression of some maturation markers : specifically , MHC molecule up regulation was totally inhibited and CD 83 expression was significantly reduced , while up regulation of CD 86 , CD 80 or CD 40 was less affected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In particular , on the targeted APC , modulation of the expression of costimulatory molecules , CD 80 , CD 86 , CD 83 , and B7RP 1 , plays an important role in the effect of the ADP ribosylating CTA 1 based adjuvants on the development of tolerance or active IgA immunity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC 1 from CHB patients showed lower expression of costimulatory molecules CD 80 , CD 86 and impaired allostimulatory mixed lymphocyte reaction ( AMLR ) compared with those in normal controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Meanwhile , decreased expression of presentation molecules ( HLA DR and CD1a ) and costimulatory molecules ( CD 80 and CD 86 ) and down regulated IL 12 secretion were also observed . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After 2 more days , Mo DCs were harvested and their CD 80 , CD 86 , and CD 83 expression was assessed by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Markers of DC maturation ( CD 80 , CD 86 , HLA DR ) were upregulated on monocytes and macrophages ( P < . 05 ) , and discrete populations of mature DC were observed ( P < . 05 ) after CCRE inhalation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A ) There was differential expression of CD 69 , CD 80 and CD 86 exposure to sCD40L and LCD40L was ensued by the strongest % MFI changes over control . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometry analysis revealed that the addition of saliva to bone marrow cells inhibits the differentiation of DC and decreased the population of differentiated immature DC , increasing the levels of major histocompatibility complex ( MHC ) class 2 while not altering the expression of costimulatory ( CD 40 , CD 80 and CD 86 ) and adhesion ( CD 54 ) molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The interaction of CD 28 with one of the B 7 molecules ( CD 80 and CD 86 ) on professional antigen presenting cells ( APC ) is generally considered to be the most important co stimulatory signal for T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The best characterized pathway includes CD 28 , its homologue CTLA 4 and their ligands CD 80 and CD 86 . ^^^ Two amino acid substitutions ( L104E and A29Y ) gave rise to slower dissociation rates for both CD 86 and CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Microglia like cells expressed MHC class 1 , class 2 , CD 40 , CD 80 , CD 86 , and IFN gammaR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Lactobacillus exposed MDCs up regulated HLA DR , CD 83 , CD 40 , CD 80 , and CD 86 and secreted high levels of IL 12 and IL 18 , but not IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , binding of the B 7 family members CD 80 or CD 86 on professional antigen presenting cells to CD 28 on T cells is considered to provide an important costimulatory signal . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The most important costimulatory protein is the monovalent homodimer CD 28 , which interacts with CD 80 and CD 86 expressed on antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We evaluated the effect of dexamethasone on the expression and regulation of MPhi B 7 family receptors ( B 7 1 , CD 80 ; B 7 2 , CD 86 ) and on their ability to co stimulate T cells . ^^^ CBMPhi were less able to up regulate CD 80 and CD 86 than PBMPhi ( p < 0 . 05 ) . ^^^ Dexamethasone inhibited the up regulation of CD 80 , CD 86 , and HLA DR on PBMPhi and even more so on CBMPhi ( p < 0 . 05 versus PBMPhi for CD 80 and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 7 treatment of naive CD 4 ( + ) T cells induced their ability to prime monocytes , as was indicated by induction of CD 80 and CD 86 expression on monocytes cocultured with IL 7 treated naive CD 4 ( + ) T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In support of this hypothesis it has been shown that these cells appear to contain cytoplasmic stores of molecules required for this function , i . e . major histocompatibility complex class 2 ( DR ) antigen , CD 80 and CD 86 . ^^^ Cross linking of the Mac 1 molecule ( CD 18 + CD11b ) was shown to result in rapid cell surface expression of CD 80 , CD 86 and DR antigen on the surface of normal human peripheral blood neutrophils . ^^^ The level of expression of CD 80 , CD 86 and DR antigen on these large cells was comparable to , and in some cases greater than , the levels found expressed on the surface of monocytes obtained from the same donors . ^^^ Following rapid translocation onto the cell surface , CD 80 and CD 86 appeared to be colocalized within large clusters reminiscent of the supramolecular antigen clusters previously found on conventional antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results show that IFN gamma , but not IL 1beta , augmented the surface expression of CD 80 , CD 83 and CD 86 molecules without inducing IL 12 production from DCs . ^^^ When combined , IFN gamma + IL 1beta treatment profoundly up regulated the expression of CD 80 , CD 83 , CD 86 and major histocompatibility complex ( MHC ) class 2 antigens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells expressed high levels of HLA DR , CD 13 , CD 33 , and CD 86 , and low levels of CD 40 , CD 80 , CD 83 , CD 123 , CD 209 , TLR 2 and TLR 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC exposed to live EBs acquired a mature DC morphology ; expressed high levels of major histocompatibility complex ( MHC ) class 2 , CD 80 , CD 86 , CD 40 , and ICAM 1 ; produced elevated amounts of interleukin 12 and tumor necrosis factor alpha ; and were efficiently recognized by Chlamydia specific CD4+ T cells . ^^^ In contrast , UV EB pulsed DC expressed low levels of CD 40 and CD 86 but displayed high levels of MHC class 2 , ICAM 1 , and CD 80 ; secreted low levels of proinflammatory cytokines ; and exhibited reduced recognition by Chlamydia specific CD4+ T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our objectives in this study were , therefore , to determine if the expression of co stimulatory molecules , such as CD 40 , CD 80 , or CD 86 , on peritoneal / splenic / liver macrophages were altered by sepsis ( cecal ligation [ CL ] and puncture [ CLP ] or necrotic tissue injury ( CL ) alone ; and to establish the contribution of such changes to the response to septic challenge using mice that are deficient in these receptors . ^^^ The macrophages were then analyzed by flow cytometry for their ex vivo expression of CD 40 , CD 80 , CD 86 , and / or MHC 2 . ^^^ RESULTS : The expression of CD 86 and MHC 2 , but not CD 40 or CD 80 , were significantly decreased on peritoneal macrophages after the onset of sepsis or CL alone . ^^^ Alternatively , splenic macrophages from septic or CL mice did not show changes in the expression of CD 80 , CD 86 , or CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , we demonstrate that the expression of human leukocyte antigen ( HLA ) class 1 and 2 molecules , but not the co stimulatory proteins CD 40 , CD 80 and CD 86 , substantially increase during expansion of neurospheres . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Specifically , the effects of 17beta estradiol on basal and LPS induced surface staining of Class 1 and 2 MHC , as well as CD 40 , CD 80 , CD 86 , CD 152 , CD 28 , CD 8 , CD11b , Fas , FasL , and also ERalpha and ERbeta , were examined in N 9 microglial cells . ^^^ However , CD 8 , CD 86 , CD11b , and CD 28 were unaffected by estrogen , and CD 80 cell surface staining significantly increased following estrogen exposure . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A representative cell line , HB 2 , that expressed DC markers including CD 83 , CD 80 and CD 86 could be induced to produce IL 12 through CD 40 stimulation . ^^^ There was no change in expression of major histocompatibility complex class 1 and class 2 antigens , CD 83 , CD 40 , CD 4 , CD11c , CD 80 , CD 86 , CD 54 , and CD 58 , or IL 12 production in the HIV infected HB 2 cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , when analyzing DC , we found that up regulation of CD 80 , CD 86 , CD 40 , RANK and PDL 1 occurred only when peptide was co administered with LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Other monocyte surface molecules ( CD 40 , CD 80 , CD 86 , MHC class 2 ) were also determined to test the specificity of the effect on FcgammaR expression levels . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The authors report in this article that purified Tax induces DC activation involving an increase in the production of CD 80 and CD 86 mRNA in the absence of corresponding protein synthesis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Exposure of peripheral blood mononuclear cells to CpG ODN 2216 led to decreased expression of the monocyte marker CD 14 and increased expression of the dendritic cell marker CD 83 , as well as increased expression of HLA DR , CD 40 , CD 80 , and CD 86 among the monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Critical contribution of CD 80 and CD 86 to induction of anterior chamber associated immune deviation . ^^^ Expression of CD 80 , CD 86 , OX 40 ligand ( OX40L ) and CD 70 was analyzed by flow cytometry . ^^^ Contribution of CD 80 and CD 86 to induction of ACAID was determined by the administration of blocking antibodies at intraocular antigen inoculation in vivo . ^^^ TGF beta treated PEC expressed CD 80 and CD 86 but not OX40L or CD 70 . ^^^ Antigen stimulated T cells proliferated and produced IL 10 , but not IFN gamma , in response to co stimulation by TGF beta treated PEC , which was abrogated by blocking antibodies against CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The intrinsic cellular activities of the lipopeptide analogues incorporated into liposomes were monitored , in vitro , by measuring the up regulation of the cell surface markers CD 80 , CD 83 , CD 86 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
No significant changes in percentages of CD 86 and CD 80 APCs or CD4+ , CD25+ T cells were documented . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , monocytes that were precultured with CD4+CD25+ Tregs displayed limited upregulation of human leukocyte antigen class 2 , CD 40 and CD 80 , and downregulation of CD 86 compared with control treated monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
BACKGROUND : The surface expression of T cell costimulatory molecules CTLA 4 and CD 28 and their counter ligands , B 7 molecules ( CD 80 , CD 86 ) , is differentially induced for T cell activation and expansion in allergic asthma . ^^^ METHODS : Plasma concentrations of soluble CTLA 4 ( sCTLA 4 ) , CD 28 , CD 80 and CD 86 in 51 children with chronic allergic asthma with or without inhaled corticosteroid treatment , and 22 sex and age matched control subjects were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This review will discuss the mechanisms of suppression , which include the local secretion of cytokines such as TGF beta and direct cell contact through binding of cell surface molecules such as CTLA 4 on suppressor T cells to CD 80 and CD 86 molecules on effector T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The following parameters were studied : surface molecules expression ( CD 18 , CD11b , CD 44 , CD 162 , CD15s , CD 80 , CD 86 , CD 16 , CD49d , CD 29 , CD 25 , HLA DR , Toll like receptor 4 [ TLR 4 ] , CXCR 1 , CCR 2 , CCR 5 , CX3CR1 ) , oxidative burst response , monocyte platelet conjugates ( using antibodies against CD 45 , CD 14 , CD41a ) , and platelet activation ( CD62P , PAC 1 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Also , the four culture conditions generated CD11c+ DC with comparable levels of major histocompatibility complex ( MHC ) class 2 , CD 40 , CD 80 and CD 86 expression , with the exception of cells cultured under serum free conditions in the absence of IL 4 , which displayed suboptimal levels of these markers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression and functional roles of costimulatory receptors , CD 28 and cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , and their ligands , CD 80 and CD 86 , on primary mouse CD 4 ( + ) T cells after activation with different doses of Con A were studied . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression levels of CD1a , CD 80 , CD 83 , and CD 86 as expressed by mean fluorescence intensity ( MFI ) on DCs differentiated from immature DCs after culture with 1 muM of piceatannol for 2 days were enhanced and decreased endocytic activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using flow cytometry , the surface expression of receptors implicated in phagocytosis ( CD 44 , CD 36 , CD 51 , CD 61 , CD 14 ) , antigen presenting capacity ( human leukocyte antigen ( HLA ) DR ) , costimulatory molecules ( CD 80 , CD 86 , CD 40 ) and complement receptor type 3 were assessed in AM from 18 patients with COPD , 14 smokers with normal lung function and nine nonsmokers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , we show that , in addition to NK cells , CD34 / fibroblast co cultures also yield myeloid CD1a ( + ) CD 38 ( + ) CD 68 ( + ) CD 86 ( + ) HLA DR ( + ) CD 14 ( ) CD 80 ( ) dendritic cells ( DCs ) after 3 4 weeks in culture . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 3 DCs had similar morphology as plasma cells , but a different immune phenotype , namely , CD 80 ( ) , CD 86 ( ) , CD 14 ( ) , and MHC class 2 ( + ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , this was not due to an immaturity , since MDC from hepatic LN had significantly higher expressions of HLA DR , CD 80 , and CD 86 compared to MDC from inguinal LN . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 , but not CD 86 , express on cultured murine keratinocyte stem cells . ^^^ Immunostaining showed that over a 7 day culture period , KSCs expressed increasing amounts of CD 80 , while CD 86 could not be detected . ^^^ This study demonstrates that the costimulatory molecule CD 80 , but not CD 86 , may be expressed on cultured KSCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show here that the uptake of chitosan at inductive mucosal sites involves CD11b / c+ APC and that chitosan feeding increases the percentage of OX62+ dendritic cells , which up regulate the major histocompatibility complex class 2 antigens without changing the expression of costimulatory CD 80 or CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Blockade of CD 80 and CD 86 at the time of implantation inhibits maternal rejection to the allogeneic fetus in abortion prone matings . ^^^ CD28 / CTLA 4 interactions with their specific B 7 ligands ( CD 80 and CD 86 ) play a decisive role in antigenic and allogenic responses . ^^^ In the present study , abortion prone CBA / J females mated with DBA / 2 males were administered with anti CD 80 and anti CD 86 monoclonal antibodies ( mAbs ) on day 4 of gestation ( time of murine implantation ) . ^^^ We demonstrated that the combined use of anti CD 80 and anti CD 86 mAbs induced maternal tolerance to the fetus in the abortion prone CBA / J mice , and displayed expansion of the maternal CD 4 ( + ) CD25+ regulatory T cell population and up regulated expression of CTLA 4 , suggesting an active mechanism of regulatory T cells in suppressing maternal rejection to the fetus . ^^^ Together , these findings indicated that blocking CD 80 and CD 86 enhanced maternal tolerance to her fetus in mice by increasing regulatory T cell function and skewing toward a Th 2 response . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recently , we and others have demonstrated , in vitro and in vivo , that coexpression of CD 80 and CD 86 costimulatory molecules enhances the immunogenic capacity of a recombinant vaccinia virus ( rVV ) encoding different tumor associated antigens . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CTLA 4 is an essential protein in the regulation of T cell responses that interacts with two ligands found on the surface of APCs ( CD 80 and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
IL 10 deficiency caused early maturation and activation of pulsed DC ( i . e . , high CD11c , CD 40 , CD 80 , CD 83 , CD 86 , IL 1 , IL 12 , and the T cell attracting chemokine CCL27 / CTACK ) and consequently an enhanced ability to process and present Ags for a rapid and robust T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Analysis of CD11c ( hi ) dendritic cells ( DC ) reveals that infection with live L . monocytogenes induces higher levels of CD 40 , CD 80 and CD 86 expression than immunization with HKLM . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The secretion of CyC ceased , consistent with the peak of the surface expression of phenotypic markers ( CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , and major histocompatibility complex class 2 ) , characteristic for the mature DC stage , whereas the secretion of cathepsins did not correlate with the maturation stage . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
MAIN OUTCOME MEASURE ( S ) : Expression of HLA ABC , HLA DR , CD 54 , CD 40 , CD 58 , CD 80 , and CD 86 by peripheral blood ( PB ) monocytes and PF macrophages was quantitated as mean fluorescence intensities by flow cytometry . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cytokines as well as cellular surface molecule such as IL 12 , TNF alpha , CD 1 alpha , CD 83 , MHCII , CD 80 and CD 86 were detected . ^^^ CD 1 alpha , CD 83 , MHCII , CD 80 and CD 86 was high expressed in survivin DC cells , however , it was low expressed in blank DC cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although previous studies have shown that altered B 7 costimulation plays a critical role in UV irradiation induced regulation of immunity , the individual roles of the B 7 receptors ( CD 28 and CTLA 4 ) or the B 7 family members ( CD 80 and CD 86 ) have not been explored . ^^^ Next , we used mice deficient for CD 80 , CD 86 , or both in photocarcinogenesis studies to assess the relative contributions of these CTLA 4 ligands . ^^^ Double deficient mice showed significantly reduced UV induced skin tumor development , whereas CD 86 ( / ) mice produced skin cancer earlier compared with CD 80 ( / ) and control mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The results presented in this study demonstrate that the saturated fatty acid , lauric acid , up regulates the expression of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) , MHC class 2 , and cytokines ( IL 12p70 and IL 6 ) in bone marrow derived DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After spore infection , immature DCs displayed a mature phenotype ( CD 83 ( bright ) , HLA DR ( bright ) , CD 80 ( bright ) , CD 86 ( bright ) , CD 40 ( bright ) ) and enhanced costimulatory activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A differential pattern in co stimulatory molecule expression is seen ; CD 80 is unchanged but CD 86 is markedly elevated and such a change is not seen in the alternatively activated macrophage but has been previously reported in monocytes resident in the non inflamed gut . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Infectious HSV 2 induced apoptotic death , decreased expression of HLA DR , CD 40 , CD 80 , CD 83 , and CD 86 , and increased release of interleukin 6 ( IL 6 ) , tumor necrosis factor alpha ( TNF alpha ) , macrophage inflammatory protein 1alpha ( MIP 1alpha ) ( CCL 3 ) , and RANTES ( regulated on activation normal T cells expressed and secreted ) ( CCL 5 ) but not IL 12 or interferon alpha ( IFN alpha ) by macaque DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : The expression rates of CD 80 , CD 86 , CD 40 , and HLA DR in the mDCs pulsed with HBcAg were 55 % + / 26 % , 80 % + / 13 % , 70 % + / 13 % and 73 % + / 24 % respectively , significantly higher than those of the control group ( 29 % + / 25 % , 35 % + / 18 % , 44 % + / 26 % and 45 % + / 23 % respectively , all P < 0 . 05 ) . ^^^ CONCLUSION : Pulsation of mDCs in vitro by HBcAg or HBsAg enhance the expression of CD 80 , CD 86 , CD 40 , and HLA DR , and increase the ability of mDCs to stimulate the proliferation of autologous T lymphocytes . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The mRNA expression of CD 80 , CD 86 , IRAK 3 , and CCL 2 was successfully detected in all of the tissues and cell lines except for Hep G 2 cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After poly ( 1 : C ) maturation , both DC types display a marked upregulation of CD 80 , CD 83 and CD 86 and the same pattern of gene expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Two tumor cell supernatants , MDA 468 and 293T , were found to be able to affect the in vitro differentiation of DCs from monocytic precursors , leading to the generation of a distinct type of DC with markedly reduced expression of DC SIGN , downregulation of CD11c , HLA DR and CD1a , and upregulation of CD 123 , HLA ABC , CD 80 , CD 40 , CD 86 , CD 54 , CD 83 , CD 25 and CCR 7 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Phenotypic characterization of MSCs by flow cytometry showed expression of MHC Class 1 alloantigens , but minimal expression of Class 2 alloantigens and costimulatory molecules , including CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Aberrant production of soluble costimulatory molecules CTLA 4 , CD 28 , CD 80 and CD 86 in patients with systemic lupus erythematosus . ^^^ OBJECTIVE : The costimulatory interactions of the B 7 family molecules CD 80 and CD 86 on antigen presenting cells with their T cell counter receptors CD 28 and CTLA 4 modulate T lymphocyte mediated immune responses in a reciprocal manner . ^^^ We investigated the possible aberrant production of soluble ( s ) forms of the T cell costimulatory molecules CD 80 , CD 86 , CD 28 and CTLA 4 in plasma of patients with systemic lupus erythematosus ( SLE ) , an autoimmune disease arising from T lymphocyte dysregulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results showed that RPMI with TCH yielded the highest percentage of cells expressing both CD 80 and CD 86 molecules and , in contrast to other media , the higher percentage of CD86+ cells in comparison to CD80+ cells . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , we did not observe increased expression of maturation associated and functionally relevant surface antigens ( CD 14 , HLA DR , CD 40 , CD 83 , CD 80 and CD 86 ) , significant secretion of IL 12 and IFN alpha in culture supernatant , or enhanced antitumour activation of cytokine induced killer cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Activated B cells exhibited increased expression of activation markers and ligands that are critical for cross talk with T cells ( CD 19 , CD 25 , CD 80 , CD 86 , MHC 1 , MHC 2 , and CD 40 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
LC from CD40L KO mice expressed similar levels of surface molecules such as CD 40 , CD 80 , CD 86 , and MHC class 2 , compared with those from wild type mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
For example , LPS induced up regulation of CD 80 ( B7 . 1 ) and CD 86 ( B7 . 2 ) is abrogated in antigen presenting cells ( APC ) deficient in TRIF or TRAM , two adaptors that are responsible for TLR 4 mediated production of Type 1 IFN . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It was previously thought that keratinocytes did not express the CD 80 and CD 86 which provide the most important costimulatory signals for the antigen specific T cell activation . ^^^ In this study , neonatal murine keratinocytes were cultured for 7 days , the results of flow cytometry and confocal microscopy showed that CD 80 could be detected on keratinocytes , while CD 86 could not be detected all the time . ^^^ The expression level of the CD 80 mRNA amplified significantly from day 1 to day 7 , as expression of the control beta actin , but CD 86 was not detected . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
As ICAM 1 is expressed on virtually any cell , it becomes a crucial molecule for the activation of CD 8 ( + ) T cells in the absence of co stimulation provided by CD 80 and CD 86 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The DCs expressed CD11c , CD11b , and the costimulatory molecules CD 40 , CD 80 and CD 86 , characteristic of mature DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the normal adult CNS , microglial cells exist in a resting state characterized by a minimal or negative expression of MHC class 2 and the co stimulatory molecules CD 80 , CD 86 and CD 40 and exhibit a unique ramified morphology . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
As compared with soluble rPA , it was found that coculture of DC with rPA loaded microparticles stimulated higher levels of MHC 2 , CD 54 , CD 80 and CD 86 expression ( p < 0 . 05 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The aim of this study was to determine the expression of MHC class 2 and the co stimulatory molecules CD 40 , CD 80 and CD 86 in keratinocytes derived from healthy oral mucosa and oral carcinomas . ^^^ All keratinocyte lines expressed CD 40 constitutively ; by contrast , CD 80 and CD 86 were universally absent . ^^^ Loss of CD 80 and CD 86 may be one means whereby tumours escape immunological surveillance . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Following incubation with CpG oligonucleotide , Namalwa cells secreted increased amounts of TNF , IL 6 , and IL 10 and expressed the costimulator molecules CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment of DC with PS G resulted in the enhanced cell surface expression of CD 80 , CD 86 , CD 83 , CD 40 , CD 54 , and human leukocyte antigen ( HLA ) DR , as well as the enhanced production of interleukin ( IL ) 12p70 , p 40 , and IL 10 and also IL 12p35 , p 40 , and IL 10 mRNA expression , and the capacity for endocytosis was suppressed in DC . ^^^ Further , inhibition of NF kappaB by helenalin and p 38 MAPK by SB 98059 prevented the effects of PS G in the expression of CD 80 , CD 86 , CD 83 , CD 40 , CD 54 , and HLA DR and production of IL 12p70 , p 40 , and IL 10 in various degrees . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Leukemic B cells up regulated expression of CD 54 , CD 80 , CD 86 , and CD 95 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Down regulation of costimulatory ligands CD 80 and CD 86 on synovial fibroblasts was identified as one mechanism of immunosuppression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Splenic CD11c ( + ) dendritic cells ( DC ) from mice infected with malaria alone and coinfected mice showed similarly increased expression of CD 40 , CD 80 , and CD 86 , but DC from coinfected mice were unable to induce CD 4 ( + ) T cell proliferation and optimal IFN gamma production in response to the malaria antigen in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment of cells from CTLA 4 expressing tumor lines with recombinant forms of the CTLA 4 ligands CD 80 and CD 86 induced apoptosis associated with sequential activation of caspase 8 and caspase 3 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The CB DCs treated with Bryo 1+CI exhibited morphologic characteristics of mature DCs , expressed increased levels of CD1a , CD 80 , CD 83 , CD 86 , and MHC class 2 as well as enhanced ability to induce proliferation of alloreactive T cells isolated from cord blood and IFN gamma production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The activation of BMDC was characterized by studying expression of cell surface co stimulatory molecules ( CD11c , MHC class 2 , CD 40 , CD 80 , and CD 86 ) , expression of genes encoding Toll like receptors and release of IL 12 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Optimal activation of T cells requires that dendritic cells undergo a process of maturation resulting in the increased expression of costimulatory molecules , such as CD 40 , CD 86 , and CD 80 , and the production of cytokines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Intriguingly , ELD 1 cells stimulated interferon ( IFN ) gamma production by purified allogeneic CD8+ T cells in an IL 2 but not IL 12 dependent manner , but failed to stimulate CD4+ T cells due to their lack of HLA DR , CD 40 , CD 80 , and CD 86 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The adaptive immune response is dependent upon upregulation of costimulatory molecules ( UCM ) such as CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we demonstrated that WKYMVm enhanced the surface expression of CD 80 , but not that of CD 40 , CD 86 and MHC class 2 , on mouse bone marrow derived dendritic cells which is one of the essential costimulatory signals for the induction of immune responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Polymorphisms of B 7 ( CD 80 and CD 86 ) genes do not affect disease susceptibility to sarcoidosis . ^^^ BACKGROUND : B 7 proteins ( CD 80 , CD 86 ) are costimulatory molecules expressed on antigen presenting cells and are essential factors for T cell activation . ^^^ METHODS : Gene polymorphisms located in CD 80 promoter , CD 80 exon 3 and exon 8 of CD 86 were examined in 146 Japanese sarcoidosis patients and 157 healthy controls using single strand conformation polymorphism and direct sequencing . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 4 ( + ) cells were cocultured with M 1 cell ( human fibroblast ) transfectants expressing HLA DR 1 , CD 80 and CD 86 alone or in combination . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This secreted HMGB 1 is necessary for the up regulation of CD 80 , CD 83 , and CD 86 surface markers of human dendritic cells and for IL 12 production . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Checking for a possible influence on LPS induced dendritic cell maturation , we found that tetrahydro 4 aminobiopterin down regulated MHC class 2 expression and counteracted LPS induced down regulation of ICOS ligand , while expression of CD 40 , CD 86 , CD 80 , B 7 H1 , and B 7 DC remained unchanged . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Injection of R 848 and CpG oligodeoxynucleotides alone enhanced the innate immune responses in vivo as demonstrated by high serum levels of inflammatory cytokines , including IL 12p70 and IFN alpha , and increased expression of CD 80 , CD 86 , and CD 40 on CD11c ( + ) dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Exposure of a LC like cell line to ATPgammaS in the presence of LPS and GM CSF augmented the induction of 1 A , CD 80 , CD 86 , IL 1beta , and IL 12 p 40 while inhibiting the expression of IL 10 , suggesting that the immunostimulatory activities of purinergic agonists in the skin are mediated at least in part by P2Rs on APCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Notably , the NKT : NK and CD 80 : CD 86 ratios were correlated with the IL 4 : IFN gamma ratio , indicative of multiple converging modes of regulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Curcumin significantly suppressed CD 80 , CD 86 , and MHC class 2 expression , but not MHC class 1 expression , in the DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Glomerular expression of CD 80 and CD 86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis . ^^^ The participation of renal expression of CD 80 and CD 86 in the immunopathogenesis of crescentic Th 1 mediated anti glomerular basement membrane ( anti GBM ) glomerulonephritis ( GN ) has not been assessed . ^^^ Immunohistochemical staining demonstrated prominent upregulation of both molecules in glomeruli of mice with anti GBM GN , suggesting a potential role for the local expression of CD 80 and CD 86 in nephritogenic effector T cell responses . ^^^ For testing this hypothesis , control or inhibitory anti CD 80 and / or anti CD 86 mAb were administered to mice during the effector phase of the disease but after the establishment of a systemic immune response . ^^^ Anti CD 80 or anti CD 86 mAb treatment had no effect on the development of GN or infiltration of leukocytes into glomeruli ; however , administration of anti CD80 / 86 mAb attenuated glomerular accumulation of CD4+ T cells and macrophages , crescent formation , and proteinuria , correlating with reduced antigen specific skin delayed type hypersensitivity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our data show that both triptolide and Dex prevented the differentiation in immature MoDC by inhibiting CD1a , CD 40 , CD 80 , CD 86 and HLA DR expression but upregulating CD 14 expression , as well as by reducing the capacity of MoDC to stimulate lymphocyte proliferation in the allogeneic mixed lymphocyte reaction . ^^^ They blocked the maturation of MoDC as totally blocked induction of CD 83 expression and absent upregulation of CD 40 , CD 80 , CD 86 and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Vpr impaired the expression of CD 80 , CD 83 , and CD 86 at the transcriptional level without altering normal cellular transcription . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The co stimulatory interactions of the B 7 family molecules CD 80 and CD 86 on antigen presenting cells , together with their T cell counter receptors CD 28 and cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) , modulate T lymphocyte mediated immune responses in a reciprocal manner . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
There are currently seven known members of the family : B7 . 1 ( CD 80 ) , B7 . 2 ( CD 86 ) , inducible costimulator ligand ( ICOS L ) , programmed death 1 ligand ( PD L 1 ) , programmed death 2 ligand ( PD L 2 ) , B 7 H3 , and B 7 H4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , both gangliosides suppressed expression of CD 40 , CD 80 , CD 86 and major histocompatibility complex class 2 on DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
During differentiation into mature DCs , the cells exhibit de novo cell surface expression of CD 83 , CD 80 , CD 86 , CD 40 , CD 206 , CD 209 , CD120a , CD120b , and intracellular synthesis of IL 10 , increase their endocytotic capacity , and acquire characteristic stellate morphology . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We found that gp 96 peptide complexes derived from HCC cells induced the maturation of DCs by enhancing expression of human leukocyte antigen class 2 , CD 80 , CD 86 , CD 40 , and CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results showed that StxB 1 and mStx 1 , but not native Stx 1 ( nStx 1 ) , resulted in enhanced expression of CD 86 , CD 40 , and major histocompatibility complex ( MHC ) class 2 molecules and , to some extent , also enhanced the expression of CD 80 on bone marrow derived DCs . ^^^ When mice were given StxB 1 subcutaneously , the levels of CD 80 , CD 86 , and CD 40 , as well as MHC class 2 expression by splenic DCs , were enhanced . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we demonstrate that oxidized phospholipids ( ox PLs ) , which are generated during infections , apoptosis , and tissue damage , interfere with DC activation , preventing their maturation . ox PLs blocked TLR 3 and TLR 4 mediated induction of the costimulatory molecules CD 40 , CD 80 , CD 83 , and CD 86 , the cytokines IL 12 and TNF , as well as lymphocyte stimulatory capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This finding correlated with the downregulation of CD 80 , CD 86 , and CD 54 expression and the increased proportion of plasmacytoid MHC class 2 ( + ) His 24 ( + ) His 48 ( + ) DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We analyzed the co stimulatory signals , CD 80 and CD 86 on tonsillar B lymphocytes . ^^^ The expression rates of CD 80 and CD 86 in the AT group with TI > or =8 were significantly decreased compared to those with TI was less than 8 ( TI < 8 ) , as well as with those in control ( tonsillar hypertrophy ) group . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In cellular activation , the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are thought to play nearly identical roles in T cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Whereas a large proportion of Langerhans cells expressed CD 86 in the steady state , CD 40 , and CD 80 were found on a smaller percentage . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Strikingly , IFN beta treatment reduced the expression of CD 40 , CD 54 , CD 80 , and CD 86 on LC , whereas IFN beta treated splenic DC showed enhanced expression of these molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Simultaneously , GGTI 298 induces the expression of CD 80 and CD 86 costimulatory molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : House dust extract activated bone marrow derived dendritic cells were found to produce IL 6 and IL 12 in a concentration dependent manner and to increase their expression of CD 40 , CD 80 , CD 86 , and MHC class 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , we demonstrate that statins reduce cell surface expression of other immunoregulatory molecules , which include MHC 1 , CD 3 , CD 4 , CD 8 , CD 28 , CD 40 , CD 80 , CD 86 , and CD 54 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Bone marrow cells cultured in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 4 , and Flt 3 ligand ( FL ) produced immature DCs that expressed intermediate levels of major histocompatibility complex ( MHC ) class 2 , low levels of CD 80 and CD 86 molecules and displayed a high capacity of endocytosis . ^^^ Bone marrow derived DCs ( BMDCs ) matured in the presence of lipopolysaccharide ( LPS ) upregulated expression of MHC class 2 , CD 80 and CD 86 , while their phagocytic capacity was dramatically reduced . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Most lung DC expressed CD11c , CD11b , and low levels of MHC class 2 , CD 40 , CD 80 , and CD 86 , consistent with an immature myeloid phenotype . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Recent crystallographic reports have suggested that it may form lattice like arrays on the cell surface upon binding B7 . 1 / B7 . 2 ( CD 80 , CD 86 ) molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We observed that treatment of DC with HRV 14 ( R DC ) , a member of the major group HRV family , diminished their T cell stimulatory capacity and induced a promiscuous and deep anergic state in cocultured T cells despite high levels of MHC molecules as well as costimulatory molecules , e . g . , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , and independent of inhibitory soluble factors such as IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Freshly isolated LP DC expressed variable but generally low levels of CD 40 , CD 80 and CD 86 , which were up regulated by activation with LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 costimulatory molecules regulate crescentic glomerulonephritis by different mechanisms . ^^^ BACKGROUND : CD 80 and CD 86 costimulatory molecules have been shown to affect the induction of Th 1 mediated crescentic antiglomerular basement membrane ( GBM ) antibody initiated glomerulonephritis ( GN ) . ^^^ The aim of the current studies was to define the mechanisms by which CD 80 and CD 86 regulate the development of this disease . ^^^ METHODS : Anti GBM GN was induced in CD 80 / , CD 86 / , and CD80 / 86 / mice , as well as in C57BL / 6 controls . ^^^ To examine whether costimulation by OX 40 ligand compensates for the absence of CD 80 and CD 86 in inducing GN , OX 40 ligand was blocked in wild type and CD80 / 86 / mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
WSPG significantly enhanced the expression of co stimulatory molecules ( CD 80 and CD 86 ) and major histocompatibility complex ( MHC ) 2 , as did LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : The immunophenotypic analysis showed that in comparison with those in the control group and the LPS group the expressions of the costimulatory molecules , Ia ( d ) , CD 80 , and CD 86 , of the DCPs were significantly weaker in the MMF group and MMP + LPS group . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We observed that DCs undergo full maturation in response to Salmonella infection , as indicated by up regulation of cell surface marker proteins CD 80 , CD 83 , CD 86 , and human leukocyte antigen class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
On encounter with MUC 1 , similar to the encounter with LPS , immature DCs increase cell surface expression of CD 80 , CD 86 , CD 40 , and CD 83 molecules and the production of IL 6 and TNF alpha cytokines but fail to make IL 12 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In response to CD 40 ligand and tumor necrosis factor alpha , the cells acquire a mature phenotype of dendritic cells that is characterized by up regulation of human leukocyte antigen ( HLA ) ABC , HLA DR , CD 80 , CD 86 , CD 40 , and CD 54 and appearance of CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Then , we tested MUTZ 3 cells against a wider panel of sensitizers and irritants on a broader spectrum of cell surface markers ( HLA DR , CD 40 , CD 54 , CD 80 , CD 86 , B 7 H1 , B 7 H2 , B 7 DC ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , incubation of DC for 48 h with ESA did not modify the expression of costimulation or maturation markers ( CD 83 , CD 40 , CD 80 , CD 86 or HLA DR ) , but induced a decrease in CCR 6 expression associated with an increased expression of CCR 7 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Concurrent to low GTP levels , a dose dependent reduction in the expression of CD 80 , CD 86 , CD 40 , CD 54 and CD 83 was seen which was accompanied by a decreased capacity of DC to stimulate T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , we report that HIFU treatment can cause the release of endogenous danger signals ( ATP and hsp 60 ) and exposure of dendritic cells ( DCs ) and macrophages to the supernatants of HIFU treated tumor cells leads to an increased expression of co stimulatory molecules ( CD 80 and CD 86 ) with enhanced secretion of IL 12 by the DCs and elevated secretion of TNF alpha by the macrophages . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Pollen up regulated the expression of CD 54 , CD 80 , CD 83 , CD 86 , HLA DR , CCR 7 , and CD 40 on DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although SERM exposed DC exhibited increased ability to internalize Ags , they were hyporesponsive to bacterial LPS : relative to control DC , they less efficiently up regulated the expression of MHC class 2 , CD 86 , and to a lesser extent , CD 80 and CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Conditioning of DCs with CSE also suppressed LPS mediated induction of CD 40 , CD 80 , and CD 86 , and suppressed maturation associated CCR 7 expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Transfection of CD 154 was functional in that it led to upregulated expression of CD 80 , CD 86 , ICAM 1 and MHC class 2 ( HLA DR ) on the B CLL cells and induction of allogeneic immune responses in MLR assays . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Compared with control transduced DC ( Ctrl DC ) , Dex DC expressed lower CD 40 , CD 80 and CD 86 but equivalent human leucocyte antigen DR . ^^^ Nevertheless , upon stimulation of lipopolysaccharide ( LPS ) or CD 40 ligand , the expression of CD 40 , CD 80 , CD 83 and CD 86 was upregulated on Ctrl DC but not on Dex DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Oxidized beta 2 GPI caused DCs to mature so that CD 83 appeared and CD 80 , CD 86 , human leukocyte antigen D region related ( HLA DR ) , and CD 40 increased . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
METHODS AND RESULTS : AGE BSA induced DCs maturation accompanied with increased expressions of CD1a , CD 40 , CD 80 , CD 83 , CD 86 , and MHC class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Upregulation of CD 40 , CD 80 , CD 83 or CD 86 on alveolar macrophages after lung transplantation . ^^^ BACKGROUND : Alveolar macrophages ( AMs ) are known to be poor antigen presenting cells , and lack the accessory molecules such as CD 40 , CD 80 or CD 86 to activate T cells . ^^^ RESULTS : Both CD 86 and CD 80 , as well as CD 83 , a marker of dendritic cells , were enhanced for most AMs during Grade A 2 and A 3 rejection episodes . ^^^ CONCLUSIONS : AM phenotypes showed marked changes during early or late acute rejection episodes , acquiring CD 80 , CD 83 and CD 86 , while CD 40 expression was further enhanced . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression levels of costimulatory molecules ( CD 80 and CD 86 ) , but not of CD 54 and B 7 H1 , were down regulated by EPF . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Increased numbers of B cells expressing the costimulatory molecules CD 80 and CD 86 were also observed in the N . caninum infected mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Neuroblastoma cells transiently transfected to simultaneously express the co stimulatory molecules CD 54 , CD 80 , CD 86 , and CD137L generate antitumor immunity in mice . ^^^ AGN2a cells nucleofected to express the co stimulatory molecules CD 80 and CD 86 expressed higher levels of these molecules than cells that had been permanently transfected with these same plasmid vectors , and the nucleofected cells were as effective as the permanently transfected cells at inducing an antitumor response in vivo in a tumor prevention model . ^^^ AGN2a cells nucleofected with four separate plasmid vectors encoding CD 54 , CD 80 , CD 86 , and CD137L induced a T cell immune response in vitro and served as a potent tumor vaccine in the tumor prevention model . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Regardless of the maturation cocktail used , all DCs possessed the characteristic phenotype of mature , migratory DCs ( high expression of CD 40 , CD 80 , CD 83 , CD 86 , CCR 7 , MHC class 1 and MHC class 2 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , B CLL DCs generated from the 2 CLL subgroups up regulated MHC 2 , CD 80 , CD 86 , CD 83 , CD 40 , and CD 54 and down regulated CD 206 in response to stimulation with a cocktail of cytokines ( CyC ) and secreted increased levels of tumor necrosis factor alpha , interleukin ( IL ) 8 , IL 6 , IL 12 ( p 70 ) , and RANTES in a manner typical of mature normal DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
SIgA also triggers migration of DC to the T cell rich regions of PP , and regulates expression of CD 80 and CD 86 on DC in PP , mesenteric lymph nodes , and spleen . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Culture of immature DCs with exogenous Gal 9 markedly increased the surface expression of CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , and HLA DR in a dose dependent manner , although Gal 9 had no or little effect on differentiation of human monocytes into immature DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although both CD8alpha+ and CD8alpha subsets increase CD 86 expression , CD 40 was preferentially up regulated on CD8alpha+ DC , and CD 80 was preferentially increased on CD8alpha DC . ^^^ In addition , high levels of CD 80 and CD 86 were found on CD11c ( int ) CD11b+ cells that accumulated in infected organs . ^^^ Rather , infection induced up regulation of CD 80 , CD 86 , and CD 40 on CD11c expressing cells of mesenteric lymph nodes was dependent on TNFR type 1 ( TNFRI ) signaling . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results showed that compared with mature DCs ( mDCs ) generated at high dose of GM CSF plus additional stimulation by lipopolysaccharide , iDCs expressed significantly lower levels of MHC 2 , CD 80 and CD 86 , and their ability to uptake FITC Dextran was stronger but the ability of stimulating proliferation of allogeneic T cells were weaker . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Human TSLP activates thymic CD11c positive dendritic cells to express high levels of CD 80 and CD 86 . ^^^ This induction depends on peptide major histocompatibility complex class 2 interactions , and the presence of CD 80 and CD 86 , as well as interleukin 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The FcRH4+ memory cells are morphologically distinctive large lymphocytes that express the CD 69 , CD 80 , and CD 86 cell activation markers . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We analyzed 72 formalin fixed and paraffin embedded sequential heart allograft biopsies from 22 patients by quantitative real time reverse transcriptase polymerase chain reaction for the mRNA expression of the costimulatory molecules CD 28 , CD 80 , CD 86 , CD 40 , and CD 154 . mRNA expression levels were correlated to histological grade and time point of rejection . mRNA expression of costimulatory molecules predominantly expressed by antigen presenting cells ( CD 80 , CD 86 , CD 40 ) , correlated with histological grade of cell mediated acute rejection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , our results demonstrate that CD 80 , CD 86 , and PD L 1 molecules are also expressed on T cells that have been activated by simultaneous exposure to anti CD 3 and anti CD 28 mAbs , but PD L 2 and GL 50 molecules were not detectable during the first six days of culture that follow such stimulation . ^^^ Additionally , we provide evidence indicating that the CD 80 , CD 86 , and PD L 1 molecules are functional , since T cells expressing B 7 family molecules are able to stimulate the proliferation of highly purified allogeneic or autologous T cells . ^^^ Anti CD 80 , anti CD 86 , and soluble CD 28 Ig protein could significantly attenuate the proliferation of T cells , whereas anti PD L 1 mAb may lead to the expansion of activated T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The EC 109 DC cells could proliferate slowly in vitro and highly expressed CD 80 , CD 83 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The Nef protein of HIV 1 induces loss of cell surface costimulatory molecules CD 80 and CD 86 in APCs . ^^^ We now show that Nef also redistributes the costimulatory molecules CD 80 and CD 86 away from the cell surface in the human monocytic U 937 cell line as well as in mouse macrophages and dendritic cells . ^^^ Furthermore , HIV 1 infected U 937 cells and human blood derived macrophages show a similar loss of cell surface CD 80 and CD 86 . ^^^ Nef colocalizes with MHC class 1 ( MHCI ) , CD 80 , and CD 86 in intracellular compartments , and binds to both mouse and human CD 80 and CD 86 . ^^^ Some Nef mutants defective in MHCI down modulation , including one from a clinical isolate , remain capable of down modulating CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The production of TNF alpha and nitric oxide ( NO ) , the expression of surface molecules CD 80 and CD 86 , and the ability of monocytes to phagocyte bacteria were assessed , in order to evaluate the effect of alginate on cell functionality . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In particular , modulation of the expression of co stimulatory molecules on the targeted APC ; CD 80 , CD 86 , CD 83 and B7RP 1 , play important roles for the effect of the ADP ribosylating CTA 1 based adjuvants for the development of tolerance or active IgA immunity . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results showed that as the disease progresses from before onset to early disease and to late disease , there is a parallel increase in surface markers of B cell activation ( IA / IE , CD 44 , CD 69 , CD 80 and CD 86 ) , in B cell proliferation , and in cell surface and serum IgE . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Treatment of the animals with baicalein , an herbal extract with anti inflammatory and numerous other effects , downregulated the microglia markers CD 68 , CD 80 , and CD 86 , all of which were upregulated in untreated mutant animals . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 are important in the initiation of T cell immunity . ^^^ We have addressed this issue using HLA DR 1 transfectants co expressing CD 80 , CD 86 , or both molecules as stimulators for nave , memory , and activated human CD4+ T cells . ^^^ Both CD 80 and CD 86 efficiently costimulated alloresponses by unseparated peripheral blood CD4+ T cells ; however , CD 86 was substantially inferior in costimulating alloresponses by separated memory T cells , and completely incompetent in costimulating three human T cell clones . ^^^ These results indicate that CD 80 and CD 86 mediate distinct signals in previously activated T cells , and demonstrate that CTLA 4 ligation may dominate the outcome of CD 86 mediated costimulation of activated CD4+ T cells . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Omp 18 induced higher expression of the B 7 ( CD 80 and CD 86 ) costimulatory molecule after 18 hours indicating processing and presentation of the antigen on the surface by bone marrow derived DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
At this time point , the number of myeloid dendritic cells and expression of CD 80 and CD 86 in lungs were decreased compared with three challenges . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Whereas CTB only delivered the Ag to MZ DC , the ADP ribosyltransferase activity of CT was required for the maturation and migration of DC to the T cell zone , where these cells distinctly up regulated CD 86 , but not CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The aim of the study was to estimate the expression of cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 , CD 152 ) , CD 28 , B7 . 1 ( CD 80 ) , and B7 . 2 ( CD 86 ) molecules on peripheral blood cells in patients with Graves ' disease ( GD ) ( n = 55 , mean age 15 . 5 + / 5 . 1 years ) and nontoxic nodular goiter ( NTNG ) ( n = 55 , mean age 15 . 2 + / 4 . 5 years ) , in comparison with sex and age matched healthy control subjects ( n = 55 , mean age 15 . 2 + / 3 . 9 years ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The main co stimulatory pathway involves the interaction of CD 80 and CD 86 , expressed on the APCs , with their T cell counter receptor , CD 28 and CTLA 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The costimulatory molecules CD 80 , CD 86 and OX40L are up regulated in Aspergillus fumigatus sensitized mice . ^^^ Results showed up regulation of both CD 86 and CD 80 molecules on lung B cells from Af sensitized mice ( 79 % CD86+ and 24 % CD80+ ) and Af / rIL 4 treated mice ( 90 % CD86+ and 24 % CD80+ ) compared to normal controls ( 36 % and 17 % , respectively ) . ^^^ The up regulation of CD 80 , CD 86 and OX40L molecules on lung B cells and macrophages from Af allergen exposed mice suggests a major role for these molecules in the amplification and persistence of immunological and inflammatory responses in ABPA . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We showed that monocyte derived DC can be productively infected , which results in maturation of the DC judged by the up regulation of CD 80 , CD 83 , CD 86 , and HLA class 2 molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression levels of CD1a , CD 80 , CD 83 , CD 86 and HLA DR as expressed by mean fluorescence intensity ( MFI ) on DC differentiated from immature DC after culture with 1 . 0 microg / ml of SUL 1 for 1 day were enhanced and decreased endocytic activity . ^^^ Cell surface expression of CD 80 , CD 83 and CD 86 on SUL 1 treated DC was inhibited by anti DC SIGN mAb , while anti DC SIGN mAb had no influence on allogeneic T cell proliferation by SUL 1 treated DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Toxoplasma gondii antigen was shown to be taken up by these cells and to increase class 1 and class 2 major histocompatibility complex ( MHC ) , CD 40 , CD 80 and CD 86 surface expression . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OBJECTIVE : This study investigated the effects of lumiliximab on allergen induced immune responses from atopic subjects compared with blocking HLA DR and costimulatory molecules , CD 80 and CD 86 . ^^^ METHODS : Allergen stimulated PBMCs from atopic subjects were pretreated with lumiliximab or antibodies to CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
At 2 and 14 d of reperfusion , the following were assessed 1 ) properties of BMMo derived DCs ( i . e . , the amount of generated DCs , differentiation state markers [ CD11c , CD 80 , CD 86 , and Ia ] , and functional state [ MLR and amount of IL 12 produced ] ) , and 2 ) the presence of DCs in the kidney . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , both wild type meningococcal LOS and KDO ( 2 ) lipid A , significantly up regulated CD 80 , CD 83 and CD 86 and released significantly higher amounts of IL 12p70 , IL 6 , IL 10 , TNFalpha , MCP 1 , IP 10 and RANTES . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometry showed polyclonal DC populations after expansion that consisted of CD11c+CD11b+ and CD11c CD11b+ cells that co expressed CD 40 , CD 80 , CD 86 , and MHCII . eDCs were functional in mixed lymphocyte reactions and produced tumor necrosis factor alpha on phytohemagglutinin stimulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC differentiated from monocytes in the presence of apoA 1 showed a decreased expression of surface molecules such as CD1a , CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We examined cell surface expression of five B 7 costimulatory molecules ( B 7 H1 , B 7 DC , B7h , CD 80 and CD 86 ) in human oral squamous cell carcinoma ( SCC ) lines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulation of cells with gliadin , in contrast with other tested food proteins , led to enhanced expression of maturation markers ( CD 80 , CD 83 , CD 86 , and HLA DR molecules ) and increased secretion of chemokines and cytokines ( mainly of IL 6 , IL 8 , IL 10 , TNF alpha , growth related oncogene , MCP 1 , MCP 2 , macrophage derived chemokine , and RANTES ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Lewis rat hearts were transplanted heterotopically into wild type or IL 12 , CD 80 or CD 86 deficient C57BL / 6 mice . ^^^ Wild type recipients were treated with CD 80 or CD 86 neutralizing Ab with and without daily cyclosporin A ( CsA , 15 mg / kg ) . ^^^ Finally , blockade of CD 86 but not CD 80 rendered xenograft recipients sensitive to daily CsA therapy , leading to indefinite xenograft survival . ^^^ Furthermore , we demonstrate that CD 80 and CD 86 have opposing roles in regulation of xenotransplantation rejection , where CD 80 drives CMR and attenuates AVR while CD 86 drives AVR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These cells were found to express CD 29 , CD 44 , CD 90 , CD 95 , CD 105 , CD 166 , and MHC class , but not CD 14 , CD 34 , CD 40 , CD 45 , CD 80 , CD 86 , CD 117 , CD 152 , or MHC class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The immunophenotype of cells altered from CD34+ , CD 38 , CD 40 , CD 80 , CD 86 , MHC 1 , MHC 2 , F4 / 80 to CD 34 , CD38+ , CD40+ , CD80+ , MHC I+ , MHC II+ , F4 / 80 ( low ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Despite the fact that viral infection resulted in phenotypic maturation of moDCs , as shown by the upregulation of cell surface markers and antigen presenting molecules ( MHC 1 and 2 , CD 80 , CD 83 , CD 86 , CD 38 ) , RSV infected moDCs showed a severely impaired capacity to stimulate CD4+ T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , DC overexpressing CD40L secreted considerably more IL 12 and expressed higher levels of the co stimulatory molecules , CD 80 , CD 86 and CD 40 , than did DC overexpressing LacZ , 4 1BBL or RANKL . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
GILZ is necessary and sufficient for DEX , IL 10 , and TGFbeta modulation of CD 80 , CD 83 , CD 86 , immunoglobulin like transcript ( ILT ) 3 , and B 7 H1 expression by DCs , and alteration of DC functions . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , for the expression of CD 83 and costimulatory molecules such as CD 40 , CD 80 , and CD 86 , no synergistic effects were observed upon stimulation with Nod plus TLR agonists . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The VIP / PACAP generated DCs are CD11c ( low ) CD45RB ( high ) , do not up regulate CD 80 , CD 86 , and CD 40 following LPS stimulation , and secrete high amounts of IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These CD11c+ DCs did not express CD 123 , CD1a , DC marker ( CMRF 56 ) , CD 40 , CD 80 , or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show that the failure of T lymphocytes to respond to Salmonella infected DC is not simply due to Salmonella induced programmed DC death or interference with up regulation of costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , the delivery of sLAg via escheriosomes enhanced the expression of costimulatory signals ( CD 80 and CD 86 ) as determined in peritoneal macrophages obtained from BALB / c mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
This loss of APC function correlates with a reduced expression of costimulatory molecules , including programmed cell death ligand ( PD L ) 1 , PD L 2 , CD 40 , CD 80 , CD 86 , and inducible T cell costimulator , and of major histocompatibility complex class 2 on CD11c ( + ) APCs from the airways of ISS treated mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
T reg cell depleted mice had increased numbers of pulmonary myeloid DCs with elevated expression of major histocompatibility complex class 2 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
AS Bcl 2 ODNs induced splenomegaly in association with increased serum IL 12 , expression of CD 80 , CD 83 , CD 86 , and CD 27 , which was attenuated by methylation of the CpG motifs of AS Bcl 2 , suggesting the involvement of immunomodulatory effect of AS Bcl 2 through pDC and B cell although methylated CpG failed to negate the increased antitumor effect of AS Bcl 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Murine bone marrow derived DC efficiently phagocytosed tumor lysate and after the uptake , the phenotype of TP DC was surprisingly comparable to unpulsed DC ( UP DC ) , exhibiting lower levels of CD 80 ( < 51 % ) , CD 86 ( < 43 % ) , and MHC class 2 ( < 59 % ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
At 2 and 14 days of reperfusion , the following properties of PBMo derived DCs were assessed : the amount of generated DCs , surface markers [ CD11c , CD 80 , CD 86 , and MHC 2 ( IA ) ] , and functional status including magnitude of mixed lymphocyte reaction ( MLR ) , production of IL 12 p 70 by DCs , and production of IFN gamma and IL 4 by DC stimulated T cells . ^^^ After 14 days of reperfusion , there was no between group differences in the numbers of DCs derived from PBMo , MLR , expression of CD 80 , CD 86 , and MHC 2 ( IA ) , and production of IL 12 , IFN gamma , and IL 4 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In comparison to DC differentiated with FCS supplementation , DC generated under serum free conditions ( sfDC ) have a more homogeneous phenotype with higher expression of IL 12 and the differentiation and activation markers CD11c , CD 40 , CD 80 , CD 83 , CD 86 , DEC 205 , and MHC class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Studying the status of accessory molecules implicated in T cell stimulation by M , it could be shown that B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) remained unchanged , whereas CD 40 was down regulated and CD 54 ( =ICAM 1 ) slightly up regulated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : After six days of culture , the monocytes developed significant dendritic morphology and a portion of cells expressed CD1a , CD 80 and CD 86 , features of DCs . ^^^ TNFalpha treatment induced DCs maturation and up regulation of CD 80 , CD 86 and CD 83 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Direct examination of the serosal disposed layers showed a significant fraction of the DCs to express DEC 205 / CD205 , CD11c , Langerin / CD207 , Fcgamma receptor / CD16 / 32 , CD 14 , and low levels of activation markers , CD 25 , CD 80 , CD 86 , and CD 95 . ^^^ In vivo , these DCs responded to two microbial stimuli , systemic LPS and oral live bacteria , by up regulating CD 80 , CD 86 , DEC 205 , and Langerin within 12 h . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Splenic DC strongly up regulated CD 86 and CD 40 but not CD 80 as soon as 8 h after CLP . ^^^ In contrast , lymph node DC equally increased the expression of CD 86 , CD 40 , and CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , heptanol significantly inhibited the LPS plus IFN gamma induced up regulation of the other costimulatory ( i . e . , CD 80 and CD 86 ) and MHC class 2 molecules expressed by BMDCs , and it significantly reduced their allostimulatory capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , NYT dose dependently increased the surface expression of major histocompatibility complex ( MHC ) class 2 ( MHC 2 ) , CD 40 , CD 54 , CD 80 , and CD 86 by murine bone marrow derived DCs and triggered their robust production of IL 1beta , IL 6 , IL 12 , tumor necrosis factor alpha , and macrophage inflammatory protein 1alpha . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Herein , we provide evidence to support the hypothesis that dendritic cells derived from NOD mice and engineered ex vivo to exhibit suppressed expression of the CD 40 , CD 80 , and CD 86 costimulatory molecules motivate an increase in the prevalence of regulatory CD 4 ( + ) CD 25 ( + ) T cells via interleukin ( IL ) 7 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It was possible to generate mature DCs from monocytes with the aid of CD40L in both cases , but decreased expression of HLA DR , CD 80 , and CD 86 markers was observed upon posttransplantation analyses in the patient with recurrent HCV . ^^^ CONCLUSION : Loss of the proliferative response as well as INF gamma and TNF alpha production , together with a decreased HLA DR , CD 80 , and CD 86 ( markers of mature DCs ) , indicated an inadequate immune response to viral progression in the liver transplant recipient with relapsing HCV infection . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of the co stimulatory molecule CD 80 but not CD 86 increased on both DC subsets when stimulated with cyclosporine . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results showed that , on B cells , GroES and GroEL stimulated the expression of CD 86 but did not induce the increase of the CD 80 expression . ^^^ GroES and GroEL also stimulated the expression of CD 80 and CD 86 on macrophages . ^^^ The same HSPs did not modify the expression of CD 80 and CD 86 on cells having characteristics of activated macrophages , the A THP 1 cell line . ^^^ DnaK did not induce any increase in the expression of CD 80 and CD 86 on lymphocytes or macrophages . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Culture of immature DCs with exogenous Gal 9 markedly increased the surface expression of CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , and HLA DR in a concentration dependent manner , although Gal 9 had no effect on differentiation of human monocytes into immature DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Different from GL S , GL M enhanced maturation of DCs in terms of upregulation of CD 40 , CD 80 , and CD 86 , and also reduced fluorescein isothiocyanate dextran endocytosis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , significant amounts of costimulatory molecules CD 40 , CD 80 and CD 86 could be detected on nmDC that expressed more CD 80 compared with omDC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Interestingly , in contrast to mature DCs derived from TNF alpha induced immature DC cultures without PsL EGFmAb , immature DCs treated with PsL EGFmAb for 7 days were completely blocked their maturation , as evidenced by decreased expression of costimulatory molecules CD 80 , CD 86 , and CD 83 , inhibited production of IL 12 , and inability to activate nave T cells in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Type 1 myeloid ( CD11c ( + ) , CD11b ( + ) , B 220 ( ) , CD8alpha ( ) ) DC made up the largest ( 40 45 % ) population and all DC expressed low levels of CD 80 , CD 86 , and CD 40 , and had high endocytic activity consistent with an immature phenotype . ^^^ The majority ( > 85 % ) of DC in the colon and MLN of IL 2 ( / ) mice were type 1 myeloid , and expressed high levels of MHC class 2 , CD 80 , CD 86 , CD 40 , DEC 205 , and CCR 5 molecules and were of low endocytic activity consistent with mature DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The upregulation by FSL 1 of the expression of CD 80 , CD 86 and the MHC class 2 molecule IA ( b ) was dose and time dependent on the surfaces of C57BL / 6 derived bone marrow derived dendritic cells but not on the surface of TLR 2 ( / ) derived bone marrow derived dendritic cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both DEN 2 and LAV 2 increased the expression of the phenotypic markers CD 80 , CD 86 , CD 40 , CD1a , HLA ABC and CD 83 , demonstrating cellular activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
OBJECTIVES : To compare the expression of CD 40 , CD 80 , CD 86 , MHC class 2 and intercellular adhesion molecule 1 ( ICAM 1 ) , and the ability to induce allogeneic T lymphocyte proliferation in vitro , of a CD 40 OSCC cell line , its CD40+ transfected derivative and null transfectants . ^^^ However , the CD40+ transfectants were the most immunologically potent and were the only cells to show increased expression of CD 86 ( as well as further upregulation of CD 40 and a statistically insignificant rise in CD 80 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Increased expression of CD 40 , CD 80 , and CD 86 by B cells was apparent within 24 h of exposure to C 274 and persisted for up to 1 week . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Levels of the soluble forms of CD 80 , CD 86 , and CD 83 are elevated in the synovial fluid of rheumatoid arthritis patients . ^^^ The release of soluble forms of CD 80 ( sCD 80 ) , CD 86 ( sCD 86 ) , and CD 83 ( sCD 83 ) provide a potentially powerful immunoregulatory mechanism . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
KM+ induced a smaller inflammatory reaction in the air pouch model and was able to inhibit differentiation of dendritic cells ( BMDC ) , but to induce maturation by enhancing the expression of MHC 2 , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometry revealed CD11b ( + ) / Gr 1 ( + ) as a heterogeneous myeloid suppressor cell also expressing low levels of MHC class 1 and 2 , CD 80 , CD 86 , CD 31 , and others . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In this study , we show that BLS stimulates bone marrow dendritic cells from mice in vitro to up regulate the levels of costimulatory molecules ( CD 40 , CD 80 , and CD 86 ) and major histocompatibility class 2 Ag . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Under explant culture system , SHPS 1 Fc treatment suppressed the expression of CD 80 and CD 86 of LCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here , we show that dendritic cells ( DC ) derived from CD patients homozygous for this mutation respond normally to purified Toll like receptor ( TLR ) ligands but fail to up regulate the costimulatory molecules CD 80 and CD 86 in response to the NOD 2 ligand muramyl dipeptide ( MDP ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It profoundly increased CD 40 , CD 54 , CD 80 , CD 86 , and MHC class 2 expression in murine bone marrow ( BM ) derived myeloid DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results demonstrate that , in contrast to the rwt virus , rM51R M virus induced the maturation of myeloid DC ( mDC ) populations , as indicated by an increase in the surface expression of CD 40 , CD 80 , and CD 86 as well as the secretion of interleukin 12 ( IL 12 ) , IL 6 , and type 1 IFN . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
When activated via TLRs , antigen presenting cells ( APCs ) release elevated levels of pro inflammatory cytokines ( TNF alpha , IL 1 , IL 6 , IL 8 , and IL 12 ) , chemokines , and nitric oxide ( NO ) and show increased expression of co stimulatory molecules ( CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cytotoxic T lymphocyte antigen 4 ( CTLA 4 ; CD 152 ) is a member of the immunoglobulin gene superfamily with strong homology to the receptor CD 28 with which it shares the ligands CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
PR 3 treated DCs express high levels of CD 83 , a DC restricted marker of maturation , CD 80 and CD 86 , and HLA DR . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
EtOH reduced surface expression of costimulatory molecules ( CD 40 , CD 80 , CD 86 ) but not that of coinhibitory CD 274 ( B 7 H1 ) on resting or CpG stimulated DC subsets . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Artificially enforced expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on tumor cells renders them more immunogenic by triggering the CD 28 receptor on T cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Further culture of myeloid cells in serum free medium with GM CSF and IL 4 generated cells that had typical dendritic morphology ; expressed high levels of MHC class 1 and 2 molecules , CD1a , CD11c , CD 80 , CD 86 , DC SIGN , and CD 40 ; and were capable of Ag processing , triggering naive T cells in MLR , and presenting Ags to specific T cell clones through the MHC class 1 pathway . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Gastric epithelial cells ( GEC ) play an important role during the host response , and their expression of class 2 MHC and costimulatory molecules such as CD 80 and CD 86 suggests their role in local Ag presentation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Perfusate MDC expressed less DC LAMP ( P = 0 . 000 ) , CD 80 ( P = 0 . 000 ) , CD 86 ( P = 0 . 003 ) , and CCR 7 ( P = 0 . 014 ) than mature hepatic lymph node ( LN ) MDC , and similar CD 86 ( P = 0 . 140 ) and CCR 7 ( P = 0 . 262 ) as and more DC LAMP ( P = 0 . 007 ) and CD 80 ( P = 0 . 002 ) than immature blood MDC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Stimulation with H . pylori induced maturation of DC , i . e . up regulation of the chemokine receptors CCR 7 and CXCR 4 and the maturation markers HLA DR , CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The early activation marker CD 69 was induced at 4 h in NK cells > B cells > T cells > monocytes whereas at 24 h CD 80 and CD 86 levels were augmented on monocytes and IL 12 was induced ; HLA DR levels increased on both B cells and monocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Dendritic cells generated from monocytes from EBC patients showed a significantly lower expression of the phenotype associated antigens CD1a , CD 83 , CD 80 , CD 86 and CD 54 than the dendritic cells from healthy controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Lupus DCs showed an altered ex vivo costimulatory profile , with a significant increase in the expression of CD 40 , decreased expression of CD 80 and CD 54 , and normal expression of CD 86 . ^^^ DCs from young lupus prone NZM 2410 mice , before the development of the disease , expressed normal levels of CD 80 and CD 86 but already overexpressed CD 40 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the present study , MUTZ 3 derived DC ( MuDC ) showed high level expression of HLA class 2 molecules , CD 80 and CD 86 , and were able to function as potent antigen presenting cells as previously reported . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , impaired expression of co stimulatory molecules including CD 80 , CD 86 , and CD40L may contribute to low polyfunctional CD4+ T cell as well CD8+ T cell activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Co culture resulted in DC with reduced expression of MHC class 2 , CD 86 , and CD 80 , and poor T cell stimulatory capacity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The surface molecules CD 25 , CD 40 , CD 80 and CD 86 were up regulated upon incubation of the B CLL cells with IMT 504 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expressions of surface marker CD 40 , CD 80 , and CD 86 in the GM CSF treatment group were also higher than those of the control group . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
HSP70 ) induced maturation of bone marrow derived dendritic cells ( DCs ) of wild type ( WT ) C57BL / 6 mice as evidenced by an increase in surface expression of MHC class 1 and 2 molecules and costimulatory molecules such as CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immunostimulation was evaluated in vitro by flow cytometric measurements of the CD 80 and CD 86 activation markers found on CD19+ murine splenocytes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We show in this report that JEV infection of mouse brain astrocytes results in induction of the nonclassical MHC Class Ib genes , H 2T23 , H 2Q4 and H 2T10 in addition to MHC 1 , Type 1 ( alpha / beta ) IFNs , TAP 1 , TAP 2 , Tapasin , LMP 2 , LMP 7 and LMP 10 but not IFNgamma , CD 80 , CD 86 and MHC 2 genes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The VLP induced an increase in expression of CD 40 , CD 80 and CD 86 but required an adjuvant , CpG DNA oligo deoxy nucleotides ( ODN ) motifs , to enhance these responses . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
C . jejuni induced the maturation of dendritic cells over 24 h , as indicated by up regulation of cell surface marker proteins CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
F . tularensis LVS also induced in a dose dependent manner an up regulation in the expression of the costimulatory molecules CD 80 and CD 86 and of CD 40 and the major histocompatibility complex class 2 molecules on dendritic cells from wt and TLR 4 / but not TLR 2 / mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Cells matured normally ; up regulation of CD 40 , CD 80 , CD 83 , CD 86 and HLA DR was not affected by adenoviral transduction . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The ability of eosinophils to enhance cytokine generation was independent of their source ( blood vs airway ) , activation by GM CSF , or detectable expression of human leukocyte antigen ( HLA ) DR , CD 80 , or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression levels of CD1a , CD 80 , CD 83 , CD 86 and HLA DR on T cadinol primed dendritic cells or calamenene primed dendritic cells were enhanced with a concomitant decrease in endocytic activity . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Although expression levels of DC associated surface antigens , including CD 80 , CD 83 , and CD 86 , were significantly higher on HHV 6 infected monocyte derived DCs than on DCs derived from mock infected monocytes , antigen presenting capacity was significantly lower in the former group . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
These results demonstrate that genes associated with phagocytosis ( CD 36 , CD 206 , and CD 209 ) are decreased and genes associated with proinflammatory chemokines ( CCL 20 , CCL 5 , and CCL 19 ) , cytokines [ interleukin ( IL ) 27 , IL 23A , IL 12A , and IL 12B ] , and costimulatory molecules ( CD 40 , CD 54 , CD 80 , and CD 86 ) are increased . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We showed that both gangliosides impaired spontaneous LC maturation induced by a short in vitro culture , as assessed by significant down regulation of co stimulation ( CD 40 , CD 54 , CD 80 , CD 86 ) and maturation markers ( CD 83 , CCR 7 ) , which correlated to an impaired ability of the cells to mount allogeneic T cell proliferation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Using flow cytometry , the cells were analyzed for the expression of costimulatory molecules , such as CD 80 , CD 86 , and CD 152 ( CTLA 4 ) . ^^^ CONCLUSION : These results suggest that LIMC over expressing costimulatory molecules such as CD 80 and CD 86 appears to play a role in the pathogenesis of AIH . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
While purified CD123+ plasmacytoid dendritic cells from adults up regulated co stimulatory molecules CD 80 and CD 86 with IL 3 alone those from neonates required the addition of CpG DNA to reach adult levels . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The frequency of circulating MDC and PDC , and the expression of CD 83 , CD 123 , CD 80 , CD 86 , and CD 40 were analyzed by flow cytometry . ^^^ The expression of CD 83 , CD 80 , and CD 86 was lower in PPMS patients . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : Immature in vitro derived DC exhibited robust phagocytic activity , and mature DC demonstrated high expression of CD 80 , CD 83 , CD 86 and the chemokine receptor CCR 7 , important for DC migration to local lymph nodes . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Integration of CD 28 and CTLA 4 function results in differential responses of T cells to CD 80 and CD 86 . ^^^ CD 80 and CD 86 have the capacity to either stimulate or inhibit T cell responses through their receptors CD 28 and cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) . ^^^ Blockade of CD 80 and CD 86 in autoimmune disease settings has revealed distinct outcomes , yet the differential functions of CD 80 and CD 86 are still unclear . ^^^ Both CD 80 and CD 86 demonstrated the capacity to costimulate T cell proliferation . ^^^ Once cell division had been initiated , all T cells undergoing cell division expressed CTLA 4 , irrespective of whether CD 80 or CD 86 costimulation was used . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Flow cytometric analysis of draining lymph node DC costimulatory molecules and activation markers from infected mice indicated a lack of upregulation of major histocompatibility complex class 2 , CD 80 , CD 86 , and CD 40 during infection , consistent with a tolerizing condition . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 , CD 83 , CD 86 and HLA DR expression on naive and stimulated myeloid dendritic cells from peripheral blood were analyzed . ^^^ No differences in CD 80 , CD 83 , CD 86 and HLA DR expression were found on naive and stimulated myeloid dendritic cells from HCC patients and healthy controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Of note , we found that CD 83 , CD 80 , CD 86 , CD 40 , and interleukin 12 upregulation were significantly impaired on K ( 5 ) 1 . 3 and K ( 5 ) 1 . 5 blockade . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The immunostaining revealed positivity for CD 45 , CD 20 , CD 22 , CD79a , IgA 2 , CD 80 , CD 86 , CD 74 , and BCL 6 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , this increased uptake did not result in an increase in expression of CD 80 or CD 86 on the surface of the MoDCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Hyperthermia increases the expression of co stimulatory molecules such as CD 80 and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Immature monocyte derived DC ( iMDDC ) failed to undergo phenotypic ( CD 80 , CD 83 , CD 86 ) or functional ( T cell activation ) maturation in response to exposure to DEP ( 0 . 001 100 mug / ml ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
A fourth cell line , unable to form tube like structures in Matrigel , produced large granulocytic cells expressing CD11b and CD 86 but not CD11c and CD 80 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The expression of interferon gamma ( IFN gamma ) , Interleukin 12p40 ( IL 12p40 ) , costimulatory molecules CD 40 , CD 80 and CD 86 and MHC class 2 were up regulated in DLN of CIA induced C57BL / 6 mice . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We have previously demonstrated that CD4+ CD25+ natural regulatory T cells ( Treg cells ) induce down modulation of CD 80 and CD 86 ( B 7 ) molecules on dendritic cells ( DCs ) in vitro . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In the presence of apigenin , CD 80 , CD 86 , and major histocompatibility complex class 1 and 2 molecules , expressions on DC were significantly suppressed , and lipopolysaccharide ( LPS ) induced interleukin ( IL ) 12 expression was impaired . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The co stimulatory molecules MHC 2 , CD 40 , CD 80 , and CD 86 were expressed in DC aggregates . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : The DC from CHB patients had a lower expression of costimulatory molecules CD 80 , CD 86 and impaired allogeneic mixed lymphocyte reaction capacity compared to those from normal controls . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Additionally , lung DC from mice infected for 7 days showed increased expression of the maturation markers CD 80 , CD 86 , and major histocompatibility complex class 2 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 5 ( ) CD 4 ( ) CD 86 ( ) CD 80 ( ) CD134L ( + ) VDCs from infected , but not noninfected , rats spontaneously released large amounts of interleukin 12 ( IL 12 ) and tumor necrosis factor alpha , whereas all VDC subsets released comparable levels of IL 10 and IL 2 cytokines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The results showed that MPA not only inhibited the expression of major histocompatibility complex ( MHC ) class 2 and costimulatory molecules CD 80 and CD 86 but upregulated B 7 DC expression on mature DC induced by LPS . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The rAAV altered DC displayed higher levels of CD 80 , CD 83 , CD 86 , and CD 1a than control DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In addition , the costimulatory molecules CD 80 and CD 86 were up regulated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
We observed high levels of CD 80 expression by Gr 1+CD11b+ cells from spleen , ascites , and tumor tissue of mice bearing 1D8 ovarian carcinoma , whereas CD 40 and CD 86 were absent . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Both LPS and CT stimulated increases in CD 80 and CD 86 expression on OVA+CD8alpha ( low ) DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Kinetic studies showed a subset and tissue specific expansion of cDC and upregulation of CD 80 and CD 86 on splenic and mesenteric lymph node ( MLN ) cDC after intragastric infection . ^^^ Expansion of pDC was more prolonged than cDC , and pDC upregulated CD 86 and MHC 2 , but not CD 80 , in both the spleen and MLN . cDC were an important source of IL 12 but not TNF alpha during infection , while pDC made neither of these cytokines . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
DC were incubated with PL resulting in up regulation of MHC 2 , CD 40 , CD 80 , and CD 86 expression and production of TNFalpha and IL 12 ( p 70 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Mature PDC expressed significantly higher levels of inducible costimulator ligand ( ICOS L ) , but similar levels of CD 80 and CD 86 , when compared to mature monocyte derived DC ( moDC ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 4 ( + ) PHA blasts express MHC class 1 and 2 , and also CD 80 and CD 86 and are thus expected to serve as potent antigen presenting cells . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of costimulatory / adhesion molecules ( CD1a , CD11c , CD 40 , CD 54 , CD 80 , CD 83 , CD 86 , CD 123 , HLA class 1 and 2 ) was assessed by flow cytometry and mRNA for cytokines ( IFN gamma , IL 10 , IL 4 , TGF beta ) with real time PCR . ^^^ Results : 1 ) high expression was observed for HLA 1 and 2 class , moderate for CD 40 , CD 83 , CD 86 and low or no expression for CD 80 , CD 54 , CD1a , CD11c and CD 123 ; 2 ) we found expression of mRNA for IFN gamma , IL 10 , IL 4 and TGF beta in blasts cells ( but not in all specimens ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
When monocyte derived DCs were obtained from the patient after having recovered from the Mycobacterium avium complex infection , they expressed normal levels of CD1a and CD 80 and were negative both for CD 83 and for CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC SIGN , and that this increased after stimulation in vitro with interleukin 4 and CD 40 ligand , with enhanced expression of activation and co stimulatory molecules CD 23 , CD 58 , CD 80 , and CD 86 , and CD 22 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Our results showed that LMP 2 gene transfer did not alter the typical morphology of mature DC , and the representative phenotypes of mature DC ( CD 80 , CD 83 , and CD 86 ) were highly expressed in rAd LMP 2 DCs . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Furthermore , depending on the mouse strain used , increased expression of MHC class 2 and costimulatory molecules such as CD 40 , CD 80 , and CD 86 was associated with exposure to SFA 8 , but not Ber e 1 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
More importantly , phagocytosis of the targeting microparticles neither altered the functionality of immature DCs nor the phenotypic expression of DC specific cell surface molecules , CD 80 , CD 86 , CD 40 and CD 54 ( ICAM 1 ) , suggesting that uptake of the targeting microparticles by themselves did not induce DC maturation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In contrast , the serum levels of tumor necrosis factor alpha and interleukin 6 and spinal interleukin 6 levels were elevated , and the expression of major histocompatibility complex class 2 molecules , CD 80 , and CD 86 on splenic dendritic cells were enhanced early after infection . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Hybrid cell lines generated from three marrow derived tumour cells consistently expressed both MHC class 1 and class 2 molecules , a range of accessory and T cell co stimulatory ligand molecules , including CD 80 and CD 86 , and directly stimulated markedly enhanced T cell proliferative responses in vitro , as compared with the parent tumour cell lines . ^^^ By contrast , hybrid cells derived from three solid tumours only expressed MHC class 2 when the parent tumour cell line expressed MHC class 2 and consistently failed to express CD 80 or CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Signal two or co stimulation is mainly provided by the triggering of CD 28 on the T cell by CD 80 and CD 86 molecules on the DC . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD4+ T cell populations in the peripheral blood were analysed for the expression of co stimulatory markers CD45RO , CD 70 , CD 80 , CD 86 , CD 137 , CD137L , CD 134 , CD 152 , CD 154 and ICOS . ^^^ SLE patients showed an increased frequency of peripheral CD4+ T cells expressing high levels of CD 80 , CD 86 and CD 134 compared to healthy controls ( 7 . 1 + / 1 . 5 % versus 1 . 7 + / 0 . 9 % ; P < 0 . 005 ; 2 . 3 + / 0 . 4 % versus 1 . 0 + / 0 . 2 % ; P = 0 . 008 , 20 . 2 + / 2 . 0 % versus 10 . 6 + / 1 . 9 % ; P < 0 . 005 , respectively ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
It was found that B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) expression remained unchanged , whereas CD 40 was down regulated and CD 54 ( = ICAM 1 ) was slightly up regulated . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
After lipopolysaccharide ( LPS ) stimulation , F4 / 80+ SPMs expressed significantly higher levels of CD 86 , CD 40 , or MHC 2 than F4 / 80+ PEMs , but not CD11c , CD 80 , CD 54 , or CD 23 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The VIP / PACAP generated DCs are CD11clowCD45RBhigh , do not upregulate CD 80 , CD 86 , and CD 40 following lipopolysaccharide ( LPS ) stimulation , and secrete high amounts of IL 10 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
When triggered by specific antigen , these Tregs act back on immature DCs to block the upregulation of CD 80 and CD 86 costimulatory molecules . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
RESULTS : All the TLR agonists induced maturation of DC as evidenced by increased expression of CD 83 , CD 80 , and CD 86 . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
There was an upregulation of the costimulatory molecules major histocompatibility complex 2 , CD 40 , CD 80 , CD 86 , and CD 205 in the DC after intestinal manipulation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
However , in CD 100 / mice , dendritic cell CD 86 ( but not CD 80 ) up regulation was significantly attenuated . ^^^ Following i . p . immunization , CD 86 , but not CD 80 , promotes early Ag specific TCR transgenic DO11 . 10 CD4+ cell proliferation and IFN gamma production , suggesting that CD 100 expression enables full expression of CD 86 and consequent CD4+ cell activation . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
In vitro , NIK increases dendritic cell antigen presentation in allogeneic and antigen specific T cell proliferation assays by potently activating NF kappaB and consequently up regulating the expression of cytokines ( TNF alpha , IL 6 , IL 12 , IL 15 , and IL 18 ) , chemokines [ IL 8 , RANTES ( regulated on activation , normal T cell expressed and secreted ) , macrophage inflammatory protein 1alpha , monocyte chemoattractant protein 1 , and monocyte chemoattractant protein 3 ] , MHC antigen presenting molecules ( class 1 and 2 ) , and costimulatory molecules ( CD 80 and CD 86 ) . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
The generation of this population was paralleled by higher expression of the costimulatory molecules CD 80 , CD 86 , and B 7 DC , but not B 7 RP , PD 1 , and B 7 H1 on CD11c ( + ) CD 4 ( + ) dendritic cells whereas CD11c ( + ) CD8alpha ( + ) dendritic cells were not altered . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
CD 80 and CD 86 have been shown to play a critical role in the optimal activation of T cells . ^^^ Although these two molecules bind the same ligand , CD 28 , the question of whether CD 80 and CD 86 provide unique signals or serve redundant roles remains controversial . ^^^ Previous studies have suggested that CD 80 binding to CD 28 may be superior to CD 86 for the activation of naive CD8+ T cells . ^^^ Our study demonstrates a previously unappreciated role for CD 80 , its superiority over CD 86 in promoting CD 25 expression , increasing both the number of cells that express CD 25 and the level expressed on a per cell basis . ^^^ These findings provide new insights into the role of CD 80 vs CD 86 and have important implications for the design of vaccines and immunotherapeutics aimed at the generation of a robust CD8+ T cell response in vivo . . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Expression of major histocompatibility complex ( MHC ) class 1 molecules , MHC class 2 , CD 80 , CD 86 , CD 40 , CD 70 , B7H1 , B7DC , B7H2 , OX40L , and 4 1BBL on Y 79 cells was measured by reverse transcription polymerase chain reaction ( RT PCR ) and flow cytometric analysis . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
Moreover , ORFV IL 10 inhibited the upregulation of DC cell surface activation and maturation markers MHC 2 , CD 80 , CD 83 and CD 86 and inhibited the capacity of MoDCs to activate CD 4 ( + ) T cells in an oxidative mitogenesis assay . ^^^
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42081 and P33681	Pubmed	SVM Score :0.0
NA