Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.52103658
The CD 28 antigen has recently been shown to mediate such co stimulatory signals by interacting with the B7 / BB 1 molecule expressed on activated B cells and monocytes . 0.52103658^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.60488413
Several of the BB 1 positive fibers bound strongly in a cell to cell contact with their CD 28 or CTLA 4 ligands on the autoinvasive CD8+ T cells , as confirmed by confocal microscopy . 0.60488413^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.53274253
Recently , it has been demonstrated that CD 28 interacts with two different ligands , designated CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) . 0.53274253^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.52257727
Thus , a reduced interaction between CD 80 and CD 28 may be relevant for the induction of CD4+ CTLs . 0.52257727^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.53812801
Interactions of CD 80 and CD 86 with CD 28 and CTLA 4 . 0.53812801^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.59982637
Blockade of the interactions between CD 28 and its ligands , CD 80 and CD 86 , prolongs the survival of allografts in some transplantation models . 0.59982637^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.52989475
CD 80 and CD 86 interact with CD 28 and deliver costimulatory signals required for T cell activation . 0.52989475^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.52024281
Blockade of the interaction of CD 28 with its ligands CD 80 and CD 86 using CTLA 4 Ig has been proposed as a therapy for a number of immune based disorders . 0.52024281^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.69368651
These findings suggest that interaction of CD 80 and CD 86 with their receptors CD 28 and CTLA 4 selectively promotes cell activation , including proliferation and IgE production in CD40+IL 4 stimulated peripheral blood mononuclear cells from atopic donors . 0.69368651^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.61663965
CD 28 binds to its ligand ( CD 80 or CD 86 ) and transduces the most important costimulatory signal . 0.61663965^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Because keratinocytes express BB 1 , a CD 28 ligand distinct from B 7 1 or B 7 2 ( which are found on professional APCs ) , we examined the possibility that the defect in IFN gamma production might be a result of nonproductive CD 28 engagement . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The CD 28 antigen on T lymphocytes provides an important co stimulatory signal to T lymphocytes and we therefore searched for the presence of its ligand , the B7 / BB 1 antigen , on blood and tonsil dendritic cells ( DC ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Antigen specific T cell activation depends on T cell receptor ligand interaction and costimulatory signals generated when accessory molecules bind to their ligands , such as CD 28 to the B 7 ( also called BB 1 ) molecule . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
A potential role for CD 28 signal transduction in thymic maturation is suggested by the demonstration that the BB 1 molecule , a natural ligand for CD 28 , is expressed on thymic stromal cells . ^^^ If the CD 28 surface receptor is simultaneously stimulated by a BB 1 expressing stromal cell , this set of interactions could lead to proliferation and positive selection . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
T cell antigen CD 28 mediates adhesion with B cells by interacting with activation antigen B7 / BB 1 . ^^^ Several mAbs were identified that inhibited CD 28 mediated adhesion , including mAb BB 1 against the B cell activation antigen B7 / BB 1 and some mAbs against major histocompatibility complex class 1 antigens . ^^^ B7 / BB 1 expression correlated closely with CD 28 mediated adhesion , but class 1 expression did not . ^^^ Transfected COS cells expressing the B7 / BB 1 antigen adhered to CD28+ CHO cells ; this adhesion was blocked by mAbs to CD 28 and B7 / BB 1 . ^^^ The specific recognition by CD 28 of the B cell activation antigen B7 / BB 1 represents a heterophilic interaction between members of the immunoglobulin superfamily that may serve to regulate T cell cytokine levels at sites of B cell activation . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The B lymphocyte / accessory cell activation antigen B 7 ( BB 1 ) has been shown in vitro to stimulate T lymphocyte proliferation and cytokine production via CD 28 present on the latter cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
These residues are adjacent to the epitope of the BB 1 antibody , which inhibits CD 28 CD80 interactions . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Here , we investigated whether in the presence of impaired responses of T cells from HIV infected individuals to signal one , costimulation through CD 28 and CD 27 after interaction with their natural ligands CD 80 and CD 70 is intact . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Proliferation was not inhibited by mAb against the CD 28 ligands BB 1 or B 7 , even though these keratinocytes express BB 1 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of CD 28 ligand B 7 1 ( as determined by BB 1 antibody ) remained unchanged in all gamma IFN transduced cell lines tested . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Monoclonal antibody BB 1 was previously shown to recognize the CD 80 protein as well as a putative B7 . 3 molecule , both ligands of the important T cell receptors CD 28 and CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The expression of the costimulatory molecule B 7 1 ( BB 1 ; CD 80 ) and its ligand CD 28 was investigated on peripheral blood ( PB ) and cerebrospinal fluid ( CSF ) T and B lymphocytes and monocytes in 11 patients with relapsing remitting multiple sclerosis ( MS ) 21 age matched healthy controls and 10 patients with central nervous system ( CNS ) infectious disease ( CID ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
METHODS : The in situ expression of B 7 1 , B 7 2 , BB 1 , and CD 28 was studied by immunohistochemistry , and B 7 1 and B 7 2 RNA expression was examined by reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of the co stimulatory molecule BB 1 , the ligands CTLA 4 and CD 28 and their mRNAs in chronic inflammatory demyelinating polyneuropathy . ^^^ To examine whether the Schwann cells in patients with autoimmune neuropathies have the potential to behave as professional antigen presenting cells , we investigated the expression of the co stimulatory molecules BB 1 , B 7 1 ( CD 80 ) B 7 2 ( CD 86 ) and their counter receptors CD 28 or CTLA 4 ( CD 152 ) at the protein and mRNA levels in sural nerve biopsies of patients with chronic inflammatory demyelinating polyneuropathy ( CIDP ) , CIDP associated with human immunodeficiency virus infection ( HIV CIDP ) , IgM paraproteinaemic neuropathy and normal or non immune axonal neuropathy . ^^^ The endoneurial T cells in the proximity of BB 1 positive Schwann cells expressed the CD 28 or CTLA 4 counter receptors . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We investigated expression of costimulatory molecules BB 1 , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and their counter receptors CD 28 and CTLA 4 ( CD 152 ) in muscle biopsy specimens of patients with scleroderma polymyositis overlap syndrome ( SSc PM ) , primary polymyositis ( PM ) , and other related diseases to examine whether the muscle fibers in patients with SSc PM behave as antigen presenting cells ( APCs ) . ^^^ The CD4+ T cells expressed the counter receptors CD 28 and CTLA 4 , and bound with the BB 1 positive muscle fibers in cell to cell contact . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The antigen specific T cell receptor can deliver the first signal , while ligation of the T cell surface molecule CD 28 by antibodies or its cognate ligands B 7 1 ( CD 80 ) or B 7 2 has been demonstrated to be sufficient for the delivery of the second signal . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Analysis of several R CR pairs ( CD 2 CD58 , CD45RO CD 22 , CD 5 CD72 , CD11a CD 54 , gp 39 CD40 , and CD 28 CD80 ) demonstrated that comparable levels of expression of the Rs ( CD 2 , CD45RO , CD 5 , and CD 28 ) and of some of the CRs ( CD 58 , CD 22 , and CD 72 ) were in all cell lines ; however , CD 54 , CD 40 , and CD 80 were expressed constitutively on the HTLV 1 and HTLV 2 infected cell lines . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
As CD 28 deficient mice have reduced basal levels of IgG 1 and IgG2a and diminished Ig class switching , we investigated whether the CD28 / B7 . 1 ( CD 80 ) ligand pairing might also be involved in human IgE regulation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Both the CD 7 and CD 3 induced elevation of phosphatidylinositol 3 , 4 , 5 trisphosphate approximately 5 10 fold less than that elicited by ligation of the costimulatory molecule CD 28 by its counter receptor CD 80 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Such cellular interactions are mediated by surface molecules including MHC class 2 Ags ( DR ) and CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Human B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) bind with similar avidities but distinct kinetics to CD 28 and CTLA 4 receptors . ^^^ B 7 0 or B 7 2 ( CD 86 ) is a T cell costimulatory molecule that binds the same receptors ( CD 28 and CTLA 4 ) as B 7 1 ( CD 80 ) , but shares with it only approximately 25 % sequence identity and is expressed earlier during an immune response . ^^^ However , CD 80 and CD 86 did not bind equivalently to CTLA 4 : CD 80 bound Y100A , a form of CTLA4lg with a mutation in the CDR 3 like region , > 200 fold better than did CD 86 ; inhibition of CD 80 mediated cellular responses required approximately 100 fold lower CTLA4lg concentrations ; and CD 80 CTLA4lg complexes dissociated 5 to 8 fold more slowly , Thus , CD 80 and CD 86 utilize different binding determinants and have different kinetics of binding to CD 28 and CTLA 4 . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
A costimulatory signal from B 7 1 ( CD 80 ) to its counter receptor CD 28 is required for T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The CD 86 gene is , therefore , located in the proximity of the CD 80 ( B7 / B7 . 1 ) gene , the first identified ligand for CD 28 and CTLA 4 , previously mapped to chromosome 3q13 . 3 q 21 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 are homologous T cell receptors of the immunoglobulin ( Ig ) superfamily , which bind B 7 molecules ( CD 80 and CD 86 ) on antigen presenting cells and transmit important costimulatory signals during T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The second , or costimulatory signal , can be provided by ligation of the B lymphocyte activation antigens B 7 1 ( CD 80 ) and B7 . 2 ( CD 86 ) to TCR antigen CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Engagement of the TCR / CD3 complex together with ligation of CD 28 by its counterstructures B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on APC are required for mitogenic T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) provides co stimulation for T cell activation by interacting with CD 28 or CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Both extracellular immunoglobin like domains of CD 80 contain residues critical for binding T cell surface receptors CTLA 4 and CD 28 . ^^^ The B 7 related molecules CD 80 and CD 86 are expressed on antigen presenting cells , bind the homologous T cell receptors CD 28 and CTLA 4 , and trigger costimulatory signals important for optimal T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Alloreactivity of CD 28 / and CD28+ / Tg gamma delta+ thymocytes could not be blocked with mAbs against CD 80 and CD 86 ligands . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Unexpectedly , tumor cells of 5 cases expressed at least one member of the B 7 family ( CD 80 , CD 86 , and B 7 3 ) and many lymphoid cells expressing the corresponding counter receptor , CD 28 , were detected in all cases . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) induce long term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro . ^^^ In this report , the co stimulatory signals provided by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) were compared to CD 28 ligation by mAb . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Both CD 28 ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) activate phosphatidylinositol 3 kinase , and wortmannin reveals heterogeneity in the regulation of T cell IL 2 secretion . ^^^ In this report , the co stimulatory signals provided by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) were compared to CD 28 ligation by mAb . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
A major co stimulatory pathway involves crosslinking the CD 28 molecule on T cells by its ligands CD 80 or CD 86 expressed on antigen presenting cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Although only 40 60 % of freshly isolated gamma delta T cells expressed CD 28 , mAbs directed against CD 28 or its ligand , CD 80 , were markedly inhibitory . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction of CD 28 with its counter receptor , B 7 1 ( CD 80 ) , on antigen presenting cells induces a co signal in T cells required to promote antigen dependent interleukin 2 ( IL 2 ) production and to prevent clonal anergy . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 ( B7 / BB 1 ) is a costimulatory molecule that serves as the ligand for two molecules expressed on T lymphocytes , CD 28 and CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 are structurally related T cell receptors for members of the B 7 ( CD 80 ) gene family , which transmit important costimulatory signals for T cell activation in vitro and in vivo . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The binding of KLH IC by normal B lymphocytes induced expression of the B7 / BB1 Ag ( CD 80 ) , the required co stimulatory ligand for T cell CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Recognition of antigen / MHC complexes by the T cell receptor delivers the first signal , while a second signal , delivered by the cell surface receptors CD 80 and / or CD 86 binding to the T cell surface molecule CD 28 , has been shown to be effective for the initiation of effective T cell responses . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The functional maturation of dendritic cells ( DC ) and other antigen presenting cells is believed to reflect the upregulation of cell surface major histocompatibility complex ( MHC ) class 2 and other T cell co stimulatory molecules , especially the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Conjugation of the T cell receptor ( TCR ) with antigen / MHC proteins must be accompanied by conjugation of T cell counterreceptors ( CD 28 or CTLA 4 ) with costimulatory molecules CD 80 or CD 86 ( B 7 1 or B 7 2 ) on antigen presenting cells ( APC ) to avert T cell anergy , and to provide essential signals for T cell activation and cytokine production . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Main costimulatory signals are provided to T cells by B 7 antigens ( CD 80 and CD 86 , expressed on antigen presenting cells ( APC ) ) upon interaction with its receptor , the CD 28 molecule expressed on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The present study compares the mitogen activated protein ( MAP ) kinase responses in T cells activated with the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 / B70 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In addition , among the antibodies for CD 80 , CD 86 , CD 28 or CD 40 , the suppression of the Th 1 clone stimulation by LC was found to occur only with anti CD 80 and anti CD 28 antibodies . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We analyzed the expression and function of the two ligands for CD 28 , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , on human Langerhans cells ( LC ) , the antigen presenting cells from epidermis . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The latter may be provided by B7 . 1 ( CD 80 ) or B7 . 2 ( CD 86 ) on APC interacting with CD 28 on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD1a+ cells differed from CD14+ cells by ( 1 ) dendritic morphology , ( 2 ) higher expression of CD1a , CD1c , CD 4 , CD 40 , adhesion molecules ( CD11c , CD 54 , CD 58 ) , major histocompatibility complex ( MHC ) class 2 molecules and CD 28 ligands ( CD 80 and CD 86 ) , ( 3 ) lack of Fc receptor FcgammaRI ( CD 64 ) and complement receptor CR 1 ( CD 35 ) expression , and ( 4 ) stronger induction of allogeneic T cell proliferation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Costimulation by the CD 28 ligand CD 80 ( B7 / B7 1 ) and CD 86 ( B70 / B7 2 ) plays an important role during T cell proliferation by augmenting synthesis of interleukin 2 ( IL 2 ) and other cytokines . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction between CD 28 on T cells with CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on APCs is considered to be of critical importance for primary T cell activation both in vivo and in vitro . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Analysis of the site of interaction of CD 28 with its counter receptors CD 80 and CD 86 and correlation with function . ^^^ Both receptors associate with the counter receptors CD 80 and CD 86 , but the avidity of interaction for CD 28 is about 20 fold lower than for CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Freshly isolated CD8+ cells could be activated ( proliferation , IL 2 production and cytotoxic activity ) by anti CD 3 presenting Fc gamma R+ mouse cells transfected with the human CD 28 ligand , CD 80 , as the only accessory signal . ^^^ On the other hand , activation of CD8+ cells by allogeneic MHC class 1 on EBV transformed B cells , which express two different CD 28 ligands , CD 80 and CD 86 , also proceeded very efficiently ( proliferation , cytotoxic activity and CD 25 expression ) , but was either not , or only partially , blocked by anti CD 80 and anti CD 86 MoAb or CTLA 4Ig . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Most important , the addition of mouse cells transfected with human B 7 1 ( CD 80 ) , a natural ligand for CD 28 , provided a potent accessory signal for GM CSF production by activated T cells , which could not be blocked by cyclosporin A . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Infection with HTLV I / II leads to constitutive expression of CD 80 and CD 86 , concomitant to down modulation of CD 28 on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CTLA 4 , a CD 28 homologue expressed on activated T cells , binds with high affinity to the CD 28 ligands , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Differential recognition by CD 28 of its cognate counter receptors CD 80 ( B7 . 1 ) and B 70 ( B7 . 2 ) : analysis by site directed mutagenesis . ^^^ In the current study , we investigated the regions of CD 28 which are involved in its interactions with CD 80 and B 70 , using site directed mutagenesis , CD 28 mAb epitope mapping , receptor based adhesion assays and direct binding of Ig fusion proteins to cell surface receptors . ^^^ Truncation or substitution of a stretch of a proline rich `` hallmark ' ' sequence , `` MYPPPY ' ' , abrogates binding to CD 80 or B 70 , while retaining CD 28 mAb epitopes and cell surface expression . ^^^ These results show that , although the same overall region on CD 28 may be involved in the interactions with CD 80 and B 70 , subtle but important differences distinguish recognition by the two molecules . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
When the reactivity of mNI 11 and mAbs binding human differentiation antigens such as CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 18 , CD 23 , CD 28 , CD 29 , CD 31 , CD 43 , CD 44 , CD45RA , CD49d , CD 50 , CD 54 , CD 58 , CD 80 , CD 102 , CD 106 , HLA class 1 , or HLA class 2 antigen was compared , no mNI 11 reactivity resembling that of these mAbs was found . mNI 11 markedly induced homotypic cell aggregation of U 937 cells when they were stimulated with LPS . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We studied the presence of the molecules CD 28 and CD 80 involved in the processes of interaction and activation of T and B lymphocytes . ^^^ The CD 28 molecule was found in all the T lymphocytes , while the CD 80 molecule was weakly expressed on the B lymphocyte membrane . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Subcellular localization of CD 80 receptors is dependent on an intact cytoplasmic tail and is required for CD 28 dependent T cell costimulation . ^^^ We wanted to determine how monomeric CD 80 could cross link CD 28 since CD 80 is expressed as a monomer on the surface of APC . ^^^ To test whether the interaction of CD 80 with the cytochalasin B sensitive cytoskeleton was necessary for T cell costimulation through CD 28 , we constructed a tailless form of CD 80 and generated stable transfectants of Chinese hamster ovary epithelial cells and Reh B cells expressing either the tailless or wild type CD 80 molecules . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Thus , CD 18 mAb inhibited the apoptotic response to IL 2 deprivation , whereas mAb against other adhesion molecules ( CD 28 , CD 29 , CD49d , CD 80 , CD 86 ) did not . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
When the reactive patterns between mNI 58A and the mAbs to various human differentiation antigens ( CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 18 , CD 23 , CD 28 , CD 29 , CD 31 , CD 43 , CD 44 , CD45RA , CD 50 , CD 54 , CD 58 , CD 80 , CD 102 , CD 106 , HLA class 1 and class 2 antigen ) were compared , that of mNI 58A was found to be similar to those of the leukocyte function associated antigen 1 ( LFA 1 ) mAbs . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
T lymphocyte receptors CD 28 and CTLA 4 bind costimulatory molecules CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on antigen presenting cells and regulate T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Nef down regulated IL 2 , but not IL 4 produced upon induction by combinations of mitogens that mimicked TcR signals together with CD 28 mAb or CD 28 ' s natural ligand ( CD 80 and CD 86 ) . ^^^ However , the co signals provided by CD 28 to up regulate IL 2 induction were unaffected by Nef , since IL 2 produced by nef transfected cells was proportionally enhanced to the same extent as that of control cells , either upon stimulation by the CD 28 mAb or CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The B 7 receptors , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , on the Ag presenting cell ( APC ) , interact with T cell CD 28 or CTLA 4 to deliver a costimulatory signal , which is particularly important for Th 1 activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) provide costimulation through CD 28 , their ligand on the T cell . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
On the other hand , the fact that detectable expression of CD 80 , ligand for CD 28 , was not found on IFN gamma treated HGF may at least in part explain the ineffective function of HGF as APC . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Two distinct signals are required to activate T cells : an Ag specific signal and a costimulatory signal mediated primarily by B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) through interactions with CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 and CD 86 ( B 7 1 and B 7 2 ) are the ligands on antigen presenting cells ( APCs ) which bind CD 28 and deliver the costimulatory signals necessary for T cell activation . ^^^ We created a mutant version of CTLA 4 Ig that could selectively bind CD 80 and block CD 28 CD80 interaction but leave CD 28 CD86 binding intact . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The costimulatory signal through CD 80 or CD 86 to its counterreceptor CD 28 or CTLA 4 on T cells has been shown to play an important role in the induction of T cell mediated immunity against tumors . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
From those findings we conclude that human monocytes use CD 80 as a costimulatory ligand for CD 28 and utilize other costimulatory mechanisms besides those mediated via molecules of the B 7 family . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The results suggest that T lymphocytes can not be activated locally in the testis of BALB / c and young NOD mice because of the absence of the necessary CD 28 ligands , CD 80 and CD 86 , from the APCs and because of the suppression of T lymphocytes by the testicular products . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of CD 80 ( B7 / BB 1 ) and CD 28 in human white blood cells treated with urocanic acid . ^^^ The present investigation was instituted to disclose any effect of UCA isomers on the cellular expression of the costimulatory antigens CD 80 ( B7 / BB 1 ) and CD 28 . ^^^ CD 80 expression was efficiently induced in monocytic ( CD14+ ) cells by human interferon gamma , while CD 28 levels on lymphocytes remained unchanged , as detected by flow cytometry . ^^^ The results obtained suggest that the mode of action for epidermal UCA induced tolerogenesis may not involve modulation of CD 80 ( B7 / BB 1 ) or CD 28 expression . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 co stimulatory regimes differ in their dependence on phosphatidylinositol 3 kinase : common co signals induced by CD 80 and CD 86 . ^^^ CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) ligation of CD 28 provide co stimulatory signals required for optimal lymphokine production in response to TCR zeta CD 3 ligation . ^^^ In this study , we have investigated whether CD 28 associated Pl 3 kinase is required for CD 80 and CD 86 co stimulation , as well as in co signaling that involves different primary signals ( i . e . ^^^ In the presence of anti CD 3 , ligation of CD 28 by both CD 80 and CD 86 was found to induce Pl 3 kinase recruitment and IL 2 production . ^^^ These data indicate that TCR zeta CD 3 dependent CD 80 and CD 86 co signaling requires Pl 3 kinase binding to the CD28pYMNM motif , while phorbol ester and lonomycin can bypass this requirement in CD 28 co stimulation . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Interaction of the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) molecules on antigen presenting cells with the receptors CD 28 and CTLA 4 on T cells generates signals important in the regulation of immune responses . ^^^ We also constructed artificial adhesion receptors on the cell surface using two different CD 28 specific sFvIgs fused to the CD 80 cytoplasmic and transmembrane domains . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , costimulatory proteins on antigen presenting cells , bind to CD 28 on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CTLA 4 is a costimulation receptor that binds to the same ligands , CD 80 and CD 86 , as CD 28 with high affinity and is transiently expressed on the cell surface of activated T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of CD 28 , CTLA 4 , CD 80 , and CD 86 molecules in patients with autoimmune rheumatic diseases : implications for immunotherapy . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The contribution of specific B T cell contact mediated events to the diminished cord B cell response in this system , using mAbs to CD 40 , CD 28 , CD 80 , and CD 72 , were investigated , as well as regulation of B cell Ig production by cytokines . alphaCD 72 ligation increased cord B cell activation and IgM production , but did not affect adult B cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction of CD 28 with B 7 molecules ( CD 80 or CD 86 ) is an essential second signal for both the activation of CD4+ T cells through the T cell receptor and the prevention of anergy . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) binds both CD 28 and CTLA 4 with a low affinity and very fast kinetics . ^^^ The structurally related T cell surface molecules CD 28 and CTLA 4 interact with cell surface ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on antigen presenting cells ( APC ) and modulate T cell antigen recognition . ^^^ Preliminary reports have suggested that CD 80 binds CTLA 4 and CD 28 with affinities ( Kd values approximately 12 and approximately 200 nM , respectively ) that are high when compared with other molecular interactions that contribute to T cell APC recognition . ^^^ In the present study , we use surface plasmon resonance to measure the affinity and kinetics of CD 80 binding to CD 28 and CTLA 4 . ^^^ At 37 degrees C , soluble recombinant CD 80 bound to CTLA 4 and CD 28 with Kd values of 0 . 42 and 4 microM , respectively . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In addition to an antigen specific signal , T cell activation requires an antigen independent costimulatory signal provided by interaction of CD 28 with B 7 ( CD 80 and CD 86 ) on the APC . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
When the reactivity of mNKES and mouse mAbs recognizing the human adhesion associated antigen ( CD 10 , CD11a , CD11b , CD11c , CD 14 , CD 16 , CD 18 , CD 23 , CD 28 , CD 29 , CD 31 , CD 43 , CD 44 , CD45RA , CD 50 , CD 54 , CD 58 , CD 80 , CD 102 , CD 106 , and HLA class 1 , and HLA class 2 antigen ) with various cell lines was compared , mNKES reactivity was found to be unique , not resembling that of any of these mouse mAbs . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Lack of CD 28 costimulation through CD 80 and CD 86 molecules on APC might play a causative role in anergy induction , as previously shown with T cell clones . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Considerable experimental data now suggest that this costimulatory signal is generated predominantly by CD 28 when engaged by its ligands CD 80 ( B 7 1 ) and / or CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction of CD 28 with one of the B 7 molecules ( CD 80 and CD 86 ) on professional antigen presenting cells ( APC ) is generally considered as the most important co stimulatory signal for T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 ( B7 . 1 ) , the ligand of CD 28 , is an important co stimulatory molecule on antigen presenting cells that delivers an essential second signal for T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on APC provide a major costimulatory signal through interactions with CD 28 on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Ligation of CD 28 on T cells with its natural ligands B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) provides a major costimulatory signal for T cells and is of potential importance for tumor rejection . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction between CD 28 and its ligands , CD 80 and CD 86 , is crucial for an optimal activation of antigen specific T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The uses of soluble competitors for CD 28 ( CTLA 4 Ig ) and agonists for the inhibitory receptor CTLA 4 ( CD 80 Ig ) offer therapeutic possibilities to tailor autoimmune responses in nonpathogenic directions . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The CD 28 ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , are expressed on human tonsillar B cells , and their expression is up regulated by IL 4 , IL 13 , and / or an anti CD 40 monoclonal antibody ( mAb ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 on T cells and CD 80 and CD 86 on APC are key molecules in the maintenance and breakdown of anergy . ^^^ This suggests that the actual regulatory mechanisms of this pathway in autoimmunity is much more complex , because of the existence of two receptors ( CD 28 and CTLA 4 ) and two ligands ( CD 80 and CD 86 ) and the opposite function of CD 28 and CTLA 4 in T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
These libraries were used to screen a blocking monoclonal antibody raised against B 7 1 ( CD 80 ) , a human cell surface antigen that binds two T cell receptors , CD 28 and CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The expression of HLA DR , CD 40 , CD40L , CD 80 , and CD 28 was investigated on thyroid samples from 30 patients ( Graves ' disease , n = 16 ; benign toxic adenoma , n = 8 ; multinodular goiter , n = 6 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Hence , the binding of CD 28 on T cells to both CD 80 and CD 86 ligands represents a crucial initial costimulatory step leading to accelerated graft rejection . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Best understood of these costimulatory interactions are those between CD 28 and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The second , costimulatory signal can be provided by cell surface molecules on antigen presenting cells such as B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , which interact with CD 28 on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In these in vivo studies , CD 28 costimulation did not show a significant benefit , possibly because of the high level expression of CD 80 and CD 86 on the surface of the lymphoma cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 25 ( IL 2 receptor alpha chain ) and CD 28 , characteristic of T cells , were detected on the surface of these cells ; this latter finding rises the possibility that enterocytes could be activated by IL 2 and / or via CD 28 through binding to its ligands CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Blockade of the ligands for CD 28 and CTLA 4 , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , revealed distinct and nonoverlapping function . ^^^ These findings suggest complex and distinct roles for CD 28 , CTLA 4 , CD 80 , and CD 86 in T cell costimulation following nucleic acid vaccination . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We found that the accessory cell activity of monocytes during LPS induced T cell proliferation is characterized by the following features : LPS primed monocytes are competent stimulators of T cell proliferation ; interaction of LPS with monocytes during the priming step is dependent on CD 14 and is sensitive to ammonia ; monocyte / T cell interactions are not MHC restricted but are strongly dependent on interactions of CD 28 and / or CTLA 4 on T cells and their ligands CD 80 and / or CD 86 on monocytes . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
METHODS : We investigated the differential roles for direct and indirect antigen presentation by preventing the CD 28 costimulatory pathway with monoclonal antibodies to rat or mouse CD 80 and CD 86 in a rat to mouse cardiac transplantation model . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CTLA 4 shares important sequence homology with CD 28 and binds to the same ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Immunoliposomes containing monoclonal antibodies ( MAbs ) to the costimulatory molecules CD 28 and CTLA 4 and their counterreceptors B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) were evaluated for the ability to increase the immune response to recombinant envelope protein rgp 120 of the MN strain of human immunodeficiency virus type 1 ( HIV 1 ) during vaccination . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
PURPOSE : To examine normal and inflamed conjunctiva from patients with ocular cicatricial pemphigoid ( OCP ) for the presence of costimulatory molecule CD 28 and its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
To elucidate the relationship between Reed Sternberg ( R S ) cells and background T cells , the expression of CD 80 and CD 86 of R S cells in Hodgkin ' s disease ( HD ) , and the ligand CD 28 expression and the MIB 1 index of background T cells were immunohistochemically investigated . ^^^ These results suggest that the interaction between CD 80 and CD 86 on R S cells , and CD 28 on background T cells may induce T cell proliferation and be associated with tumor mass of HD . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
A stimulating anti CD 28 mAb , or CD 80 or CD 86 on stably transfected P 815 cells , provided the co stimulatory signal . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
One such co stimulatory signal is delivered when CD 28 on T cells binds to CD 80 or CD 86 on antigen presenting cells ( APC ) . ^^^ Furthermore , the activity of transcription factors regulating the IL 2 promoter enhancer region including activation protein 1 , CD 28 response element and nuclear factor kappaB were 4 8 times higher after CD 80 compared to CD 86 ligation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
This was significantly correlated with the expression of CD 28 ligand , i . e . , CD 86 ( but not CD 80 ) , and with an increase in the proliferative activity ( labeling index ) of myeloma cells in bone marrow . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Despite the importance of the costimulatory proteins B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , and their counterreceptors CD 28 and CTLA 4 ( CD 154 ) in the regulation of T cell proliferation in the adult immunological system , the initial appearance of these proteins during embryonic development has not been investigated . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 binds both the CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) ligands on antigen presenting cells . ^^^ We tested the hypothesis that in vivo blockade of the CD 28 pathway via the anti CD 80 and anti CD 86 monoclonal antibodies ( mAbs ) would prolong allograft survival . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The role of costimulation in activation for proliferation and cytokine mRNA production of peripheral blood T cells was studied by blocking CD 28 , CTLA 4 , and their ligands CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Here we used tumors expressing wild type and mutant CD 80 , as well as mice with targeted mutation of CD 28 , to address this issue . ^^^ We report that in syngeneic wild type mice , B7W ( W 88 > A ) , a CD 80 mutant that has lost binding to CD 28 but retained binding to CTLA 4 , can enhance the induction of antitumor cytotoxic T lymphocytes ( CTL ) ; B7Y ( Y 201 > A ) , which binds neither CD 28 nor CTLA 4 , fails to do so . ^^^ Moreover , expression of a high level of CD 80 on thymoma EL 4 cells conveys immunity in mice with a targeted mutation of CD 28 gene . ^^^ Taken together , our results demonstrate that B 7 CTLA4 interaction enhances production of antitumor CTL and resistance to tumor challenge and that optimal enhancement of antitumor immunity by CD 80 requires its engagement of both CD 28 and CTLA4 . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In this study , we have utilized the natural ligand for CD 28 and CTLA 4 ( CD 80 ) to determine under what circumstances positive and negative effects are operative . ^^^ This inhibition is reversed by blocking with both anti CD 80 or Fab fragments of anti CTLA 4 but also requires CD 28 engagement . ^^^ In the absence of intracellular calcium elevation , CTLA 4 was not expressed at the cell surface , and CD 80 acted positively as a costimulator of T cells , via CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Unlike ligation of CD 3 , stimulation with anti CD 28 mAb or CHO cells expressing the CD 28 ligands CD 80 or CD 86 did not lead to tyrosine phosphorylation of HS 1 in Jurkat T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Therefore , a key question concerns the molecular basis for the costimulation of T cells by those tumor cells not expressing the CD 28 ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 needs to be cross linked to initiate signals , yet both of its ligands , CD 80 and CD 86 , are expressed as monomers . ^^^ Previously , we determined the cytoplasmic tail of CD 80 is required for CD 28 mediated costimulation and subcellular relocalization of CD 80 in lymphocytes . ^^^ Thus , small clusters of CD 80 found on APC are insufficient to initiate CD 28 mediated signals , and the formation of CD 80 caps appears to be a critical factor regulating the initiation of T cell costimulation . ^^^ A 30 kDa phosphoprotein that associates with the cytoplasmic tail of CD 80 in activated cells may play a role in CD 80 redistribution and thus CD 28 mediated costimulation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The costimulatory signal provided by the interaction between CD 28 and its ligands , CD 80 and CD 86 , is critical for T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Costimulatory molecules in Wegener ' s granulomatosis ( WG ) : lack of expression of CD 28 and preferential up regulation of its ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) on T cells . ^^^ Since the expression of costimulatory molecules has a significant impact on the cytokine profile and proliferation response of T cells , the goal of this study was to characterize the expression of costimulatory molecules ( CD 28 , CTLA 4 ( CD 152 ) , B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) ) on T cells , monocytes and B cells in WG , and to correlate the findings with clinical parameters such as disease activity , extent and therapy . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Ligation of CD 28 molecules expressed on the surface of human leukaemic natural killer like YT cells triggers intracellular signals leading to cytolysis of target cells expressing CD 80 or CD 86 molecules . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
To study the prerequisites of functional interactions between malignant B cells and intermingled T cells in B cell non Hodgkin ' s lymphomas ( B NHL ) , we examined the expression of CD 40 , CD 80 and CD 86 and their ligands CD 40 ligand ( CD40L , CD 154 ) , CD 28 and CTLA 4 ( CD 152 ) using immunohistochemistry and confocal laser scanning microscopy . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Further , 9 cryosections from MF lesions and 9 from SS lesions were stained for LFA , ICAM 1 , CD 40 , CD 40 ligand , CD 28 , CD 80 , CTLA 4 , CD 86 , FAS , FAS ligand , CLA and CD15s . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
This can be provided by the accessory T cell molecule CD 28 upon binding to its respective ligands B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Furthermore , expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells and cytokine levels ( IL 1alpha , IL 1beta , IL 2 , IL 6 , IL 8 , IL 10 , IL 13 , TNF alpha and IFN gamma ) in the afferent lymph were analyzed by cytofluorometry and ELISA . ^^^ However , no changes in the expression of CD1a , CD 4 , CD 8 , CD 28 , CD 54 , CD 80 , CD 86 and HLA DR on migrating lymph cells were detectable . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
While it was found that systemic administration of mouse CTLA 4 Ig could also inhibit the progression of effector immune responses when administered shortly before or during clinical disease , these were significantly more active when delivered directly into the CNS , which probably involved an action on both CD 28 ligands , CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We show here that 1 ) F 2 g3p was recognized with anti CTLA 4 mAb but not with anti CD 28 mAb , and 2 ) F 2 g3p bound to CD 80 but not to CD 86 . ^^^ In contrast , F 2 g3p weakly inhibited the binding of CD 28 with CD 80 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Since it contains a high percentage of CD 28 and CD57+ cells , is increased in size and granularity , and is transiently expressed following in vitro stimulation of naive CD8+ T cells , we speculate that this subset mainly represents recently activated effector T cells that are able to interact with CD 80 and CD 86 ( B 7 1 and B 7 2 respectively ) negative tissue cells . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 and CD 86 are not equivalent in their ability to induce the tyrosine phosphorylation of CD 28 . ^^^ Ligation of either CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) , two principal ligands for CD 28 , is thought to skew the immune response toward Th 1 or Th 2 differentiation . ^^^ Purified human peripheral T cells or Jurkat T cells were stimulated with Chinese hamster ovary ( CHO ) cells expressing either human CD 80 ( CHO CD 80 ) or human CD 86 ( CHO CD 86 ) or with anti CD 28 monoclonal antibody ( mAb ) . ^^^ In the presence of phorbol 12 myristate 13 acetate , both CHO CD 80 and CHO CD 86 , like anti CD 28 mAb , were capable of stimulating cytokine production from both human peripheral T cells and Jurkat T cells . ^^^ Several intracellular signaling proteins , such as CBL and VAV , were phosphorylated on tyrosine in response to CD 80 , CD 86 , and anti CD 28 mAb . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Co stimulatory factors B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) and their ligands , including CD 28 , are important for the efficient presentation and persistence of an antigen specific immune reaction . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 expression was generally comparable between CD3+ cells that did and did not express CD 80 or CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In these cells , induction of somatic mutations required cross linking of the surface receptor for Ag and T cell contact through CD 40 : CD 40 ligand and CD 80 : CD 28 coengagement . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Interaction of CD 28 with CD 80 or CD 86 molecules on APC initiates a costimulatory or second signal within the T cell which augments and sustains T cell activation initiated through the TCR . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
To determine whether antilymphocyte Abs to T cell costimulatory molecules are generated in patients with autoimmune diseases and , if they exist , to clarify the mechanism of their production and pathological roles , we investigated the presence of autoantibodies to CTLA 4 ( CD 152 ) , CD 28 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) in serum samples obtained from patients with various autoimmune diseases and from normal subjects using recombinant fusion proteins . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Analyses included a flow cytometric immunophenotyping of peripheral blood mononuclear cells ( CD 3 , CD 4 , CD 8 , CD 19 , CD 25 , CD 28 , CD 56 , CD 57 , CD 80 and HLA . ^^^ A significantly lower proportion of CD 8 lymphocytes and a trend for decreased CD 19 , CD 28 and CD 80 was detected among colorectal cancer patients before liver directed chemotherapy compared to healthy controls . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Since these two receptors use the same ligands , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , the co ordinate timing of CD 28 and CTLA 4 expression has a major impact on the regulation of immune responses . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of CD 80 and CD 86 on AM , and of CD 28 and CTLA 4 on T cells , was evaluated . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Enhanced expression of CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 and their ligands CD 28 and CD 154 in fulminant hepatic failure . ^^^ To define a possible role for changes in the regulation of antigen presentation in fulminant hepatic failure ( FHF ) , we studied the expression of co stimulatory molecules CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 along with their ligands CD 28 and CD 154 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In the present study we examined the expression of CD 40 , CD 40 ligand ( CD40L ) , CD 80 , CD 86 , CD 28 and cytolytic T lymphocyte associated antigen 4 ( CTLA 4 ) on lymphocytes immunohistochemically . ^^^ Positive percentage expression of CD40L , CD 28 and CTLA 4 , and CD 40 , CD 80 and CD 86 was calculated for the number of CD3+ and CD19+ cells , respectively . ^^^ While most T cells and B cells expressed CD 28 , and CD 80 and CD 86 , respectively , in gingival tissues , the expression of CD40L and CTLA 4 was lower but highly variable between specimens . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Blocking the interaction of the CD 28 costimulatory receptor with its ligands , CD 80 and CD 86 , inhibits in vivo immune responses , such as allograft rejection , and in some instances induces tolerance . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Although normal alveolar macrophages ( AMs ) did not bear or bore low levels of costimulatory molecules , AMs from sarcoid patients with CD 4 T cell alveolitis upmodulated CD 80 , CD 86 , and CD 72 and expressed high levels of interleukin ( IL ) 15 ; lymphocytes accounting for T cell alveolitis expressed Th 1 type cytokines [ interferon ( IFN ) gamma and / or IL 2 ] and bore high levels of CD 5 and CD 28 but not of CD 152 molecules . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Here , in vitro propagated DC were transduced using an adenoviral ( Ad ) vector to express the gene encoding cytotoxic T lymphocyte antigen 4 immunoglobulin ( CTLA4lg ) , which blocks interaction of CD 80 and CD 86 on DC with CD 28 on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Flow cytometric analysis demonstrates that mPEG modification dramatically decreases antibody recognition of multiple molecules involved in essential cell : cell interactions , including both T cell molecules ( CD 2 , CD 3 , CD 4 , CD 8 , CD 28 , CD11a , CD62L ) and antigen presenting cell ( APC ) molecules ( CD 80 , CD 58 , CD62L ) likely preventing the initial adhesion and costimulatory events necessary for immune recognition and response . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 interactions with either CD 80 or CD 86 are sufficient to induce allergic airway inflammation in mice . ^^^ In the present study , the importance of CD 28 as well as the individual roles of CD 80 and CD 86 were examined in this system using wild type and CD 28 knockout ( KO ) mice . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction between the T cell surface molecules CD 28 and costimulatory molecules of antigen presenting cells ( CD 80 or CD 86 ) is the most important point of the T helper cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
This overexpression of CD 80 resulted in the loss of detectable cell surface CD 28 expression on thymocytes and a significant reduction in both the surface T cell receptor expression and the ratio of CD 4 ( + ) to CD 8 ( + ) single positive thymocytes in Tg animals compared to nontransgenic ( non Tg ) controls . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Collectively , these results demonstrate the importance of costimulation for naive CD8+ T cell activation , suggest that CD 80 and CD 86 can mediate opposing effects , possibly due to differential interaction with CD 152 and CD 28 , and indicate differences in the sensitivity of immature vs mature CD8+ T cells to the induction of nonresponsiveness following costimulation deficient Ag presentation . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Here , we report a model of CD 28 costimulation using PMA plus the natural ligand CD 80 that resulted in very limited stimulation of IL 2 , as evidenced by both cytokine production and IL 2 promoter stimulation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
METHODS : Forty six patients with MS and 29 healthy individuals were analyzed by direct 2 color immunofluorescence flow cytometry for expression of CD 80 , CD 86 , CD 28 and CTLA 4 on T and B cells and monocytes . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Simultaneous triggering of the costimulatory receptor CD 28 on T cells through its natural ligand CD 80 partly corrected the CD40L defect in patients with intermediate CD 4 T counts ( 200 500 ) , but not in AIDS patients . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of costimulatory molecules CD 80 and CD 86 and their receptors CD 28 , CTLA 4 on malignant ascites CD3+ tumour infiltrating lymphocytes ( TIL ) from patients with ovarian and other types of peritoneal carcinomatosis . ^^^ The CD 28 and CTLA 4 molecules on T cells serve as receptors for the CD 80 and CD 86 costimulatory antigens . ^^^ We have examined the frequency of expression of CD 80 ( B7 . 1 ) , CD 86 ( B7 . 2 ) , CD 28 and CTLA 4 surface antigens on TIL isolated from malignant ascites or peritoneal washings of 26 patients with ovarian carcinoma and five patients with non ovarian peritoneal carcinomatosis . ^^^ We have shown that in addition to CD 28 and CTLA 4 , CD3+ intraperitoneal TIL express the costimulatory molecules CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The present review focuses on the role of two receptor ligand pairs : CD 28 and cytotoxic T lymphocyte associated antigen 4 , which interact with the B 7 ligands ( CD 80 , CD 86 ) ; and CD 40 , which interacts with the CD40L ( CD 154 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Triggering of human NK cells by CD 80 and CD 86 appeared to be independent of CD 28 and CTLA 4 , at least as determined by the reagents used in the present study , because the expression of these molecules could not be detected on the NK cell lines by either flow cytometry or in redirected lysis assays . ^^^ Thus , human NK cells may use receptors other than CD 28 and CTLA 4 in their interactions with CD 80 and CD 86 molecules . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Competitive experiments using soluble blocking peptides showed that addition of CD 28 or CD 80 peptide recovered LPS induced down regulation of CD27high expression on CD30+ T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In this study , expression of the co stimulatory ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , as well as the receptors CD 28 and CTLA 4 , were quantitated in central nervous system ( CNS ) tissues from mice at various stages of EAE . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
One is provided by T cell receptor ( TCR ) / CD3 in the context of the mayor histocompatibility complex ( MHC ) , and another signal is mediated by antigen independent molecules , that is T cell membrane bound CD 28 and its specific ligand B 7 1 ( CD 80 ) present in APC . ^^^ Our main goal was to determine whether deficiency in interferon gamma ( IFN gamma ) production shown by peripheral blood mononuclear cells ( PBMC ) from lepromatous leprosy ( LL ) patients , could be overcome by reconstituting in vitro the appropriate signals ( by means of addition of anti CD 28 and anti CD 80 monoclonal antibodies ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
It is concluded that interaction of CTLA 4 with its functional ligands , CD 80 or CD 86 , can down regulate human T cell responses , probably by intracellular signalling events and independent of CD 28 occupancy . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of the costimulator molecules , CD 80 , CD 86 , CD 28 , and CD 152 on lymphocytes from neonates and young children . ^^^ The expression of CD 80 , CD 86 , CD 28 , and CD 152 were examined on peripheral blood lymphocytes from adults , neonates ( cord blood lymphocytes ) and young children ( 2 20 months of age ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 engagement by specific monoclonal antibody ( mAb ) or binding of the natural ligands , CD 80 and CD 86 , induces tyrosine phosphorylation of CD 28 , which in turn recruits and activates the signal transducer and activator of transcription 6 ( Stat 6 ) . ^^^ The Stat 6 association with CD 28 is specifically induced by CD 80 or CD 86 ligand binding and is not dependent upon the secretion of IL 4 or IL 13 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
One signal is delivered through the TCR by engagement with peptide / MHC complexes , and the second is delivered by interaction between costimulatory molecules such as CD 28 and its ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We quantitatively measured the number of T cells expressing cell surface activation associated molecules ( CD 69 , CD 25 , CD 122 , HLA DR ) and co stimulatory molecules ( CD 28 , CTLA 4 , CD 80 , CD 86 ) , including a Type 2 T cell marker ( CD 30 ) and CD11b , by flow cytometry of skin and peripheral blood . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The co stimulatory signal provided by the interaction between CD 28 and its ligands , CD 80 and CD 86 , is critical for T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
It was found that NOD macrophages , dendritic cells , and T cells , but not B cells , expressed lower basal levels of CD 86 , but not CD 80 , CD 28 , or CD 40 , compared with C57BL / 6 and BALB / c . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We determined B lymphocytes ( CD 40 ( + ) ) , T lymphocytes ( CD 3 ( + ) ) , T helper ( CD 4 ( + ) ) , and T suppressor ( CD 8 ( + ) ) cells and the expression of costimulatory signals B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) on B cells and their counter receptors CD 28 and CTLA 4 on T cells by fluorescence activated cell sorting analysis . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
T cell costimulation is provided by ligation of CD 28 with either B7 . 1 ( CD 80 ) or B7 . 2 ( CD 86 ) on antigen presenting cells , and can be inhibited by a soluble form of CTLA 4 ( CTLA 4 Ig ) that binds to both B7 . 1 and B7 . 2 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction of the costimulatory molecule B7 . 1 ( CD 80 ) with its receptor CD 28 provides a strong positive signal to T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In mammals , the classical B 7 molecules expressed on antigen presenting cells , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) , bind the structurally related glycoproteins CD 28 and CTLA 4 ( CD 152 ) , generating costimulatory signals that regulate the activation state of T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Here we show that stimulation through the CD 28 receptor in the absence of TCR engagement with either an anti CD 28 cross linking antibody or the CD 80 ligand transiently increases expression of the insulin like growth factor 1 receptor ( IGF IR ) on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
No differences in the surface expression of the costimulatory molecules CD 28 , CD 152 or CD 154 was seen between immune and non immune donors after either 24 or 120 h of TSL incubation , nor were differences detected in the expression of the B 7 ligands CD 80 or CD 86 on CD14+ monocytes . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
To characterize the involvement of costimulatory pathways in the pathogenesis of myasthenia gravis ( MG ) , a multiparameter flow cytometry assay was adopted to enumerate blood mononuclear cells ( MNC ) expressing CD 28 , CD 80 , CD 86 , CD 40 , and CD40L molecules in patients with MG and healthy subjects . ^^^ Patients with MG had lower percentages of CD 8 ( + ) CD 28 ( + ) cells , augmented percentages of CD 4 ( + ) CD 80 ( + ) , CD 4 ( + ) CD 86 ( + ) , CD 8 ( + ) CD 80 ( + ) , CD 8 ( + ) CD 86 ( + ) , CD 14 ( + ) CD 80 ( + ) , and CD 14 ( + ) CD 86 ( + ) cells , and similar levels of cells expressing CD 40 and CD40L and of B cells expressing CD 80 and CD 86 compared to the controls . ^^^ The high percentages of CD 80 and CD 86 positive T cells and monocytes may reflect persistent activation of T and B cells , whereas the low CD 28 expression may be the result of chronic exposure to CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
As we have previously shown , CL 01 cells are IgM+ IgD+ and effectively mutate the expressed Ig VHDJH and 5 lambda J lambda genes and switch to IgG , IgA , and IgE upon B cell receptor engagement and contact with CD4+ T cells through CD 40 : CD 154 and CD 80 : CD 28 coengagement . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We observed that neither sphingomyelinase nor C 2 ceramide could costimulate human T cell proliferation in combination with anti CD 3 antibody , whereas CD 80 , a natural CD 28 ligand , was strongly costimulatory . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
They provide costimulatory signals ( in particular , CD 40 and the CD 28 ligands CD 80 and CD 86 ) necessary for naive T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The intermolecular contact regions between monomers of the homodimeric DNA binding protein ParR and the interaction between the glycoproteins CD 28 and CD 80 were investigated using a strategy that combined chemical cross linking with differential MALDI MS analyses . ^^^ Glycoprotein fusion constructs CD 28 IgG and CD 80 Fab were cross linked in vitro by DTSSP , characterized by nonreducing SDS PAGE , digested in situ with trypsin and analyzed by MALDI MS peptide mapping ( + / thiol reagent ) . ^^^ The data revealed the presence of an intermolecular cross link between the receptor regions of the glycoprotein constructs , as well as a number of unexpected but nonetheless specific interactions between the fusion domains of CD 28 IgG and the receptor domain of CD 80 Fab . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The possibility that anti CD 80 liberated CD 28 molecules that were sequestered by the T cell expressed CD 80 , enabling them to coaggregate with TCR : CD 3 complexes was excluded by finding that anti CD 80 and anti CD 152 individually caused maximal enhancement , rather than having additive effects . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
For that purpose , we analyzed the expression on PB B and T cell subsets of both the CD 28 and CD 80 costimulatory molecules , the ability of T lymphocytes to bind to exogenous recombinant IL 2 , and the serum levels of soluble CD 8 ( sCD 8 ) that might interfere with CD8+ T cell activation in a group of 10 ALC patients with active ethanol intake ( ALCET group ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Laboratory findings included lymphocytopenia , highly elevated CD45RA / CD45R0 ratio , as well as reduced expression of the co stimulatory molecules CD 154 , CD 86 , CD 80 and CD 28 on CD4+ cells in combination with disturbed lymphocyte transformation in vitro . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Costimulation light : activation of CD4+ T cells with CD 80 or CD 86 rather than anti CD 28 leads to a Th 2 cytokine profile . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Our results demonstrate that quantitation of the capacity of CD 8 ( + ) CD 28 ( ) T cells from patient circulation to suppress the activation of costimulatory molecules ( CD 80 , CD 86 ) on donor APC offers a reliable tool for monitoring specific immunosuppression against the graft in solid organ transplantation . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The crucial role of costimulatory molecules , CD 28 , CTLA 4 , CD 80 and CD 86 , for T cell activation and inhibition has been established . ^^^ In this study , we screened for polymorphisms of human CD 28 , CD 80 and CD 86 genes , and detected that polymorphisms were tested for the association with rheumatoid arthritis ( RA ) and systemic lupus erythematosus ( SLE ) . ^^^ Variations were identified in the coding regions of CD 80 ( 452G / A , 614C / G and 864A / G ) and CD 86 ( 1057A / G ) , while no variation was observed in the coding region of CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In this review , we summarize the current understanding of these complex costimulatory pathways including the individual roles of the CD 28 , CTLA 4 , B 7 1 ( CD 80 ) , and B 7 2 ( CD 86 ) molecules . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The functionally related antitumor drugs 5 , 6 dimethylxanthenone 4 acetic acid ( DMXAA ) and flavone acetic acid ( FAA ) , which cause tumor vasculature collapse and tumor necrosis , were used to attack the tumor vasculature , whereas the T cell costimulator B7 . 1 ( CD 80 ) , which costimulates T cell proliferation via the CD 28 pathway , was used to stimulate antitumor immunity . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
On the antigen presenting cell the key players are found in the extended family of B 7 genes comprising cd 80 , cd 86 , B7h / B7RP 1 and B 7 H1 . cd 80 and cd 86 encode proteins that bind to CD 28 and CTLA 4 on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Strikingly , ICOS up regulation is significantly reduced in the absence of CD 80 and CD 86 and can be restored by CD 28 stimulation , suggesting that CD 28 CD80 / CD86 interactions may optimize ICOS expression . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Female Balb / c mice were given an intraperitoneal injection of 25 microg LPS and sacrified 24 h or 72 h later to determine total cell yield , lymphocyte subpopulations ( B cells , total T cells , CD4+ and CD8+ cells ) , the costimulatory molecules CD 28 , B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) and the percentage of apoptotic cells in PP and in the spleen as well as small intestinal IgA concentration . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
For this purpose , we have generated a whole series of isogenic virus stocks ( NL 4 3 backbone ) bearing or not bearing on their surface foreign CD 28 , CD 54 ( ICAM 1 ) , CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) proteins . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Transfection of tumor cells with the gene encoding the costimulatory molecule B 7 1 ( CD 80 ) , the ligand for CD 28 and cytotoxic T lymphocye antigen 4 on T cells , has been shown to result in potent T cell mediated antitumor immunity . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Because T cell receptor engagement of major histocompatibility complex ( MHC ) molecules in the absence of costimulation mediated via CD 28 binding to CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) can lead to anergy or apoptosis , we determined whether HIV type 1 ( HIV 1 ) virions incorporated MHC class 1 ( MHC 1 ) , MHC 2 , CD 80 , or CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
OBJECTIVE : Interaction between the co stimulatory molecule B 7 1 ( CD 80 ) on antigen presenting cells and its counter receptor CD 28 on T lymphocytes plays a key role in the induction of cell mediated immune responses . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Interference with CD 28 , CD 80 , CD 86 or CD 152 in collagen induced arthritis . ^^^ We have investigated interference with co stimulation by administering mAbs towards CD 28 , CD 80 , CD 86 , and CD 152 in mice immunized for the development of collagen induced arthritis ( CIA ) . ^^^ Anti CD 80 and anti CD 86 treatment inhibited disease score and incidence , whereas anti CD 28 treatment led only to a delayed disease onset . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In the present report , we present findings on the gene sequences encoding the nonhuman primate homologues of human CD 80 , CD 86 , their ligands CD 28 and CD 152 , CD 154 , CD 95 , and CD 95 L from rhesus macaques and for phylogenetic analysis from pig tailed macaques , African sooty mangabey monkeys , baboons , and vervets as well as select molecules from the New World aotus and marmoset monkeys . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The CD 28 ligands CD 80 and CD 86 are expressed on APC , and both provide costimulatory function . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
OBJECTIVES : To investigate the expression and regulation of CD 80 , CD 86 , and CD 28 costimulatory molecules in sialoadenitis and interstitial nephritis in patients with Sjgren ' s syndrome ( SS ) . ^^^ METHODS : Expression of CD 80 , CD 86 , and CD 28 molecules was studied by immunohistochemical staining of lip biopsy specimens obtained from patients who had sialoadenitis associated with SS , and renal biopsy specimens obtained from patients who had interstitial nephritis associated with SS . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Isolated peripheral blood lymphocytes were stained for the lymphocyte subset markers CD 4 , CD 8 , CD 19 , their respective activation markers CD 25 , HLA DR , CD 38 , and the costimulatory molecules CD40L , CD 28 , CD 40 , CD 80 , and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
No differences were noted in the expression of CD 152 or CD 80 , a CD 28 and CD 152 shared ligand . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
An immunoperoxidase technique was used to examine CD 28 , CD 152 , CD 80 and CD 86 positive cells in gingival biopsies from 21 healthy / gingivitis and 26 periodontitis subjects . ^^^ In conclusion , percentages of CD 28 , CD 152 , CD 80 and CD 86 did not reflect differences in clinical status . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Some data suggest that the interaction between CD 28 and CD 80 ( B7 . 1 ) stimulates Th 1 responses and that CD 28 and CD 86 ( B7 . 2 ) stimulates Th 2 responses , however this is controversial . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The objective of this study was to examine the expression of costimulatory molecules CD 28 , B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) in conjunctival biopsies from patients with active VKC and normal controls . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Optimal T cell activation by antigen presenting cells requires co stimulatory signaling , such as the interaction of CD 80 , CD 86 , or both with CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
However , signal 2 is elicited by interaction between CD 28 and its ligands ( CD 80 and CD 86 ) and is antigen independent . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Engagement of costimulatory molecules such as CD 28 or CD 152 ( CTLA 4 ) on T cells by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) dictates the nature of T cell mediated immune responses . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 and CD 86 ( also known as B 7 1 and B 7 2 , respectively ) are both ligands for the T cell costimulatory receptors CD 28 and CD 152 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
BACKGROUND : B 7 molecules ( CD 80 , CD 86 ) and their counter receptors , CD 28 and CD 152 ( CTLA 4 ) , play an important role in T cell mediated immune responses . ^^^ However , following the nasal provocation with house dust , not only CD 86 , but also CD 80 , CD 28 , and CD 152 were significantly expressed in allergic patients . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The association of molecules like CD 80 , CD 86 ( co stimulatory molecules ) in monocytes and B cells and CD 30 , CD62L , ALL , CD11a , CD 28 , CD 124 and CD 152 in CD4+ , they have shown to be of particular interest in allergic sufferings . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Sentinel nodes ( SNs ) and non SNs were evaluated by routine pathologic analysis , and a portion of each node was processed for expression of three dendritic markers of activation ( CD 80 , CD 86 , CD 40 ) and their corresponding T cell receptors ( CTLA 4 and CD 28 ) . ^^^ Reverse transcriptase polymerase chain reaction ( RT PCR ) analyses of paired SNs and non SNs demonstrated a marked reduction in semiquantitative expression of CD 80 ( 77 % ) , CD 86 ( 77 % ) , and CD 40 ( 85 % ) , as well as CTLA 4 ( 88 % ) and CD 28 ( 85 % ) in SNs . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Both CTL and antigen presenting dendritic cells were activated as indicated by a decisive increase in their respective activation markers CD 2 , CD 25 , CD 28 as well as CD 48 and CD 80 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Major histocompatibility complex ( MHC ) class 2 expressing cells as well as CD 3 , CD 4 , CD 25 , CD 28 , CD 68 , CD 80 , and CD 83 positive cells were localized immunohistologically . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction of CD 28 and its ligands ( CD 80 , CD 86 ) on antigen presenting cells and that of TCR / CD3 MHC are required for T lymphocyte activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Although new molecules continue to be discovered , the functions of B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , CD 28 , cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , inducible costimulator ( ICOS ) , programmed death 1 ( PD 1 ) , OX 40 ( CD 134 ) and CD 40 ligand ( CD40L , CD 154 ) are now sufficiently understood that immunologists are targeting them to manipulate T cells to slow the progression of autoimmune diseases or treat tumours through the increase in T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 and CTLA 4 are opposing regulators of T cell activation , triggered by the two ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
A portion of each node was processed for expression of the dendritic markers of activation CD 80 , CD 86 , and CD 40 , and their corresponding T cell receptors CTLA 4 and CD 28 . ^^^ Reverse transcription polymerase chain reaction analyses of corresponding sections of the sentinel and nonsentinel nodes demonstrated a marked reduction in semiquantitative expression of CD 80 ( 77 % ) , CD 86 ( 77 % ) , and CD 40 ( 85 % ) , as well as CTLA 4 ( 88 % ) and CD 28 ( 85 % ) in sentinel as compared to nonsentinel nodes . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
A primary costimulatory signal is delivered through the CD 28 receptor after engagement of its ligands , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We hypothesize that expression of CD 80 and / or CD 86 on porcine cells may also play a role in NK cell activation as human NK cells express a variant of CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Surface expression of CD 25 , CD 28 , CD 40 , CD 54 , CD 80 , CD 86 and CTLA 4 was evaluated on CD 3 , CD 4 , CD 8 , CD 14 and CD 19 PBMC subpopulations by three colour flow cytometry . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The first one is an antigen dependent one via the T cell receptor , the second one is a costimulatory signal which can be delivered by the CD 28 molecule after binding to CD 80 ( B7 . 1 ) or CD 86 ( B7 . 2 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Humanized 9 . 3 blocked ligation of CD 28 Ig to cells expressing the CD 28 receptor CD 80 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The major second signal is generated by interaction of the CD 28 molecule expressed on most T lymphocytes with its natural ligands CD 80 and CD 86 located on APCs . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The ratio of CD 80 : CD 86 expression was significantly lower on CD 4 ( + ) and F4 / 80 ( + ) spleen cells in CD 28 peptide treated mice . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Upon ligation by CD 80 ( B 7 1 ) or CD 86 ( B 7 2 ) , CD 28 induces T cell proliferation , cytokine production , and effector functions , whereas CTLA 4 signaling inhibits expansion of activated T cells and induces tolerance . ^^^ In contrast to wild type CD 80 , the evolved co stimulatory molecules , termed CD 28 binding protein ( CD28BP ) and CTLA 4 binding protein ( CTLA 4BP ) , selectively bind to CD 28 or CTLA 4 , respectively . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 is an important costimulatory molecule for T cells , and it has been shown that cell surface expression of its ligands , CD 80 and CD 86 , can enhance cellular immune responses against tumor cells , however , these tumor cells do not normally express the ligands . ^^^ Many new vaccines will be based upon soluble recombinant antigens , and in vaccination with these antigens CD 80 and CD 86 would normally be expressed on activated antigen presenting cells and additional stimulation through CD 28 would not be predicted to enhance responses further . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In this study , we report the construction of a monovalent single chain Fv antibody fragment from a high affinity antihuman CD 28 antibody ( CD28 . 3 ) that blocked adhesion of T cells to cells expressing the CD 28 receptor CD 80 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We hypothesized that in relapsing remitting MS ( RRMS ) ( with or without of IFNbeta treatment ) and in secondary progressive patients ( SPMS ) IL 12 and costimulatory molecules ( CD 80 [ B 7 1 ] , CD 86 [ B 7 2 ] , CD 28 , CD 40 , CD40L ) would be differentially produced or expressed by MO or T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In correlation with their selective effects on humoral and cellular immune responses , these chemokines also differentially attract CD 4 ( + ) versus CD 8 ( + ) T cells and modulate CD 40 , CD 80 , and CD 86 expressed by B 220 ( + ) cells as well as CD 28 , 4 1BB , and gp 39 expression by CD 4 ( + ) and CD 8 ( + ) T cells in a dose dependent fashion . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Cell surface interactions between the T cell costimulatory receptors , CD 28 and cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , with their cognate ligands , CD 80 and CD 86 , on antigen presenting cells play an important role in T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction between CD 28 on T cells and CD 80 on APCs intensifies the linkage between TCR and MHC at the site of contact between T cells and APCs . ^^^ Using soluble fusion proteins ( soluble CTLA 4 , CD 28 , and CD 80 ) to block either CD 28 on the surface of T cells or CD 80 on the surface of APCs , it was demonstrated that CD 80 acquisition by T cells is mediated through its receptors , possibly CD 28 interaction . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Antibodies to the CD 28 counter ligands CD 80 and CD 86 inhibited allergen induced release of TLCA in mild asthmatic explants by 94 % ( P < . 05 ) and 62 % , but TLCA release from moderate asthmatic explants was unaffected by CTLA 4 Ig . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
High expression of CD 28 , CD 80 , CD 86 , CD 40 , CTLA 4 , CD44v variants , and FasL occurred in alopecia areata resistant mouse spleens . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 4 T cell activation is positively ( CD 28 ) and negatively ( CTLA 4 ) regulated by the costimulatory ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The CD 86 ( + ) major histocompatibility complex class 2 ( + ) ( MHC 2 ) ( + ) cells and CD 28 ( + ) CD 4 ( + ) T cells were decreased by SST treatment , while CD 80 ( + ) MHC 2 ( + ) cells , CD 40 ( + ) MHC 2 ( + ) cells and CD 154 ( + ) CD 4 ( + ) T cells showed no change . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 , CD 86 , CD 28 and Cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) are well known co stimulatory molecules that form the major co stimulatory pathway essential for full activation of T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Additional phenotyping of these clones showed them to express CD 25 , CD 28 , CD 80 and 86 but not CD 56 or 57 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of CD 4 , CD8alpha , CD 40 , CD 86 , CD 28 , CD 31 and vascular endothelial growth factor was not different between the two tumor groups but expression of CD 40 ligand , CD 80 , NK1 . 1 and CXCR 3 was relatively lower in A11 / Mig tumors . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Changes in the number of CD 80 ( + ) , CD 86 ( + ) , and CD 28 ( + ) peripheral blood lymphocytes have prognostic value in melanoma patients . ^^^ Melanoma patients with thinner tumors and those surviving revealed an increase of CD 19 ( + ) CD 80 ( + ) and CD 19 ( + ) CD 80 ( + ) CD 86 ( + ) cells , as well as a higher concentration of CD 3 ( + ) CD 4 ( + ) CD 28 ( + ) cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
A hexapeptide motif , MYPPPY , which has been supposed to be essential for the interaction with CD 80 , is present in both Woodchuck CD 28 and CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
For the majority of selected anti CD 28 affibodies , in co culture experiments involving Jurkat T cells and CHO cell lines transfected to express human CD 80 ( hCD 80 ) or LFA 3 ( hLFA 3 ) on the cell surface , respectively , pre incubation of Jurkat cells with affibodies resulted in inhibition of IL 2 production when they were co cultured with CHO ( hCD80+ ) cells , but not with CHO ( hLFA 3+ ) cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Several studies have provided evidence that activation of antigen specific T cells requires interactions between CD 28 on T cells and its ligands , CD 80 and CD 86 , on antigen presenting cells ( APCs ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Flow cytometric studies showed that T cell and antigen presenting cell adhesion molecules ( CD 2 , CD11a ) , signaling ( CD3epsilon , T cell receptor ) , and costimulatory molecules ( CD 28 , CD 80 ) were efficiently immunocamouflaged by mPEG derivatization . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CTLA4Ig binds to CD 80 and CD 86 on antigen presenting cells , blocking the engagement of CD 28 on T cells and preventing T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Porins and lipopolysaccharide from Salmonella typhimurium regulate the expression of CD 80 and CD 86 molecules on B cells and macrophages but not CD 28 and CD 152 on T cells . ^^^ RESULTS : Our results show that porins of S . typhimurium increase the expression of CD 86 and the expression of CD 80 both on B lymphocytes and macrophages , while the expression of CD 28 and CD 152 on T lymphocytes was unaltered . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of costimulatory molecules ( CD 80 , CD 86 , CD 28 , CD 152 ) , accessory molecules ( TCR alphabeta , TCR gammadelta ) and T cell lineage molecules ( CD4+ , CD8+ ) in PBMC of leprosy patients using Mycobacterium leprae antigen ( MLCWA ) with murabutide and T cell peptide of Trat protein . ^^^ This observation prompted us to study the expression of the costimulatory molecules ( CD 80 , CD 86 , CD 28 , CD 152 ) , other accessory molecules ( TCR alphabeta / gammadelta ) and T cell lineage molecules ( CD4+ and CD8+ ) during constitutive and activated state of peripheral blood mononuclear cells ( PBMC ) derived from normal and leprosy individuals using different formulations of Mycobacterium leprae total cell wall antigen ( MLCWA ) , Trat and MDP BE using flow cytometric analysis . ^^^ An increased surface expression of CD 80 , CD 86 and CD 28 but decreased CD 152 expression was observed when PBMC of normal , BT / TT ( tuberculoid ) and BL / LL ( lepromatous ) patients were stimulated in vitro with MLCWA+MDP BE+Trat peptide using liposomal mode of antigen delivery , while opposite results were obtained with the antigen alone . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We have therefore investigated associations between MS and polymorphisms in the CD 152 ( CTLA 4 ) , CD 28 , CD 80 and CD 86 genes in Australian patients . ^^^ Screening of CD 28 , CD 80 and CD 86 genes identified novel polymorphisms in the putative promoter regions of CD 28 ( 372 G / A ) and CD 86 ( exon 2 359 deletionAAG ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
As both CD 28 and CTLA 4 molecules are implicated in the function of Treg , we investigated the ability of their two natural ligands , CD 80 and CD 86 , to influence the Treg suppressive capacity . ^^^ Our data show that CD 80 and CD 86 have opposing functions through CD 28 and CTLA 4 on Treg , an observation that has significant implications for manipulation of immune responses and tolerance in vivo . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of costimulatory molecules , CD49D and CD 28 on T cells and CD 80 and CD 86 on monocytes , was unchanged . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
PBMCs expressing CD 8 , CD 28 , CD 80 , or CD 154 were significantly reduced in HCV infected patients compared with the healthy controls . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Using splenic T cells and IPE from donor mice with disrupted genes for CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , CTLA 4 , and / or CD 28 , we report that B 7 2 ( + ) IPE in the presence of anti CD 3 supported selectively the activation of CTLA 4 ( + ) CD 8 ( + ) T cells that express their own B 7 2 and secrete enhanced amounts of active TGFbeta . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
When N glycosylation was prevented through point mutations in N glycosylation sites in CD 28 , or reduced by glycosidase inhibitors , the binding of CD 28 to CD 80 significantly increased . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Murine T cells do not endogenously upregulate CD 80 expression but rather acquire CD 80 from antigen presenting cells ( APC ) during CD 28 ligation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The most widely comprehended costimulatory pathway involves the ligation of CD 28 expressed on T cells by CD 80 and CD 86 upregulated on APCs . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha and CD 80 modulate CD 28 expression through a similar mechanism of T cell receptor independent inhibition of transcription . ^^^ Ligation of CD 28 by an antibody or by CD 80 also down regulated CD 28 transcription through the same mechanism , providing evidence that CD 28 can generate a T cell receptor independent signal with a unique biological outcome . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The B 7 ligands CD 80 and CD 86 on APCs deliver either costimulatory or inhibitory signals to the T cell when interacting with their counter receptors CD 28 and CD 152 ( CTLA 4 ) on the T cell surface . ^^^ Using multivalent presentation and conditions mimicking clustering , believed to be essential for signaling through these receptors , and by applying a combined differential mass spectrometry and structural mapping approach to these conditions , we were able to identify a putative contact area involving hydrophilic regions on both CD 28 and CD 80 as well as a putative CD 28 oligomerization interface induced by B 7 ligation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Here we provide the first evidence that the insertion of CD 80 and CD 86 into HIV 1 increases virus infectivity by facilitating the attachment and entry process due to interactions with their two natural ligands , CD 28 and CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The gammadelta T cell effect upon the LNC response to MBP did not involve a change in expression of the costimulatory molecules CD 28 , CD40L , and CTLA 4 on TCRalphabeta ( + ) cells , and CD 40 , CD 80 , and CD 86 on CD 19 ( + ) and CD11b ( + ) cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Expression of the B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) molecules on the surface of leukemia cells made unnecessary the additional CD 28 costimulation of T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The co stimulatory molecule , CD 80 ( B 7 1 ) , is a ligand of CD 28 and plays a key role in the induction of cell mediated immune responses . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 28 induces immunostimulatory signals in dendritic cells via CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The aim of the study was to estimate the expression of CTLA 4 ( cytolitic T lymphocyte associated antigen 4 , CD 152 ) , CD 28 , B7 . 1 ( CD 80 ) and CD7 . 2 ( CD 86 ) molecules on peripheral blood cells in patients with Graves ' disease ( GD ) ( n=28 , mean age 15 . 4 ) , in patients with nontoxic nodular goiter ( NTNG ) ( n=28 , mean age 15 . 6 years ) in comparison with sex and age matched healthy control subjects ( n=28 , mean age 15 . 9 years ) . ^^^ The analysis of CD3+ T lymphocytes co expressing CD 152 and CD 28 antigens on peripheral blood revealed increased percentages of CTLA 4 / CD28 positive cells in patients with Graves ' disease ( p < 0 . 004 , p < 0 . 04 ) compared to the controls and euthyroid Graves ' patients , while B7 . 1 ( CD 80 ) and B7 . 2 ( CD 86 ) molecules were detected only in some hyperthyroid patients on activated monocytes . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
One of the key costimulatory receptors is CD 80 , which binds the T cell ligands , CD 28 , and CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Sections were stained with antibodies against : EG 2 ( eosinophils ) , CD 4 ( T cells ) , CD 68 ( macrophages ) , CD1a ( Langerhans cells ) , CD 28 ( on T cells ) and costimulatory molecules ( CD 80 , CD 86 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Proliferation assays and Q PCR revealed that CD 152 inhibited T cell proliferation and , at the same time , decreased CD 152 expression by secluding CD 80 and CD 86 from CD 28 engagement . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interaction of CD 28 with one of the B 7 molecules ( CD 80 and CD 86 ) on professional antigen presenting cells ( APC ) is generally considered to be the most important co stimulatory signal for T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The best characterized pathway includes CD 28 , its homologue CTLA 4 and their ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In addition , binding of the B 7 family members CD 80 or CD 86 on professional antigen presenting cells to CD 28 on T cells is considered to provide an important costimulatory signal . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The most important costimulatory protein is the monovalent homodimer CD 28 , which interacts with CD 80 and CD 86 expressed on antigen presenting cells . ^^^ This work completes the initial structural characterization of the CD 28 CTLA 4 CD 80 CD86 signaling system . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Specifically , the effects of 17beta estradiol on basal and LPS induced surface staining of Class 1 and 2 MHC , as well as CD 40 , CD 80 , CD 86 , CD 152 , CD 28 , CD 8 , CD11b , Fas , FasL , and also ERalpha and ERbeta , were examined in N 9 microglial cells . ^^^ However , CD 8 , CD 86 , CD11b , and CD 28 were unaffected by estrogen , and CD 80 cell surface staining significantly increased following estrogen exposure . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Elevation of plasma soluble T cell costimulatory molecules CTLA 4 , CD 28 and CD 80 in children with allergic asthma . ^^^ BACKGROUND : The surface expression of T cell costimulatory molecules CTLA 4 and CD 28 and their counter ligands , B 7 molecules ( CD 80 , CD 86 ) , is differentially induced for T cell activation and expansion in allergic asthma . ^^^ METHODS : Plasma concentrations of soluble CTLA 4 ( sCTLA 4 ) , CD 28 , CD 80 and CD 86 in 51 children with chronic allergic asthma with or without inhaled corticosteroid treatment , and 22 sex and age matched control subjects were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The expression and functional roles of costimulatory receptors , CD 28 and cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , and their ligands , CD 80 and CD 86 , on primary mouse CD 4 ( + ) T cells after activation with different doses of Con A were studied . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Although previous studies have shown that altered B 7 costimulation plays a critical role in UV irradiation induced regulation of immunity , the individual roles of the B 7 receptors ( CD 28 and CTLA 4 ) or the B 7 family members ( CD 80 and CD 86 ) have not been explored . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Aberrant production of soluble costimulatory molecules CTLA 4 , CD 28 , CD 80 and CD 86 in patients with systemic lupus erythematosus . ^^^ OBJECTIVE : The costimulatory interactions of the B 7 family molecules CD 80 and CD 86 on antigen presenting cells with their T cell counter receptors CD 28 and CTLA 4 modulate T lymphocyte mediated immune responses in a reciprocal manner . ^^^ We investigated the possible aberrant production of soluble ( s ) forms of the T cell costimulatory molecules CD 80 , CD 86 , CD 28 and CTLA 4 in plasma of patients with systemic lupus erythematosus ( SLE ) , an autoimmune disease arising from T lymphocyte dysregulation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The co stimulatory interactions of the B 7 family molecules CD 80 and CD 86 on antigen presenting cells , together with their T cell counter receptors CD 28 and cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) , modulate T lymphocyte mediated immune responses in a reciprocal manner . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In cellular activation , the CD 28 ligands B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) are thought to play nearly identical roles in T cell activation . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
In addition , we demonstrate that statins reduce cell surface expression of other immunoregulatory molecules , which include MHC 1 , CD 3 , CD 4 , CD 8 , CD 28 , CD 40 , CD 80 , CD 86 , and CD 54 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We analyzed 72 formalin fixed and paraffin embedded sequential heart allograft biopsies from 22 patients by quantitative real time reverse transcriptase polymerase chain reaction for the mRNA expression of the costimulatory molecules CD 28 , CD 80 , CD 86 , CD 40 , and CD 154 . mRNA expression levels were correlated to histological grade and time point of rejection . mRNA expression of costimulatory molecules predominantly expressed by antigen presenting cells ( CD 80 , CD 86 , CD 40 ) , correlated with histological grade of cell mediated acute rejection . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Here , our results demonstrate that CD 80 , CD 86 , and PD L 1 molecules are also expressed on T cells that have been activated by simultaneous exposure to anti CD 3 and anti CD 28 mAbs , but PD L 2 and GL 50 molecules were not detectable during the first six days of culture that follow such stimulation . ^^^ Anti CD 80 , anti CD 86 , and soluble CD 28 Ig protein could significantly attenuate the proliferation of T cells , whereas anti PD L 1 mAb may lead to the expansion of activated T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The aim of the study was to estimate the expression of cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 , CD 152 ) , CD 28 , B7 . 1 ( CD 80 ) , and B7 . 2 ( CD 86 ) molecules on peripheral blood cells in patients with Graves ' disease ( GD ) ( n = 55 , mean age 15 . 5 + / 5 . 1 years ) and nontoxic nodular goiter ( NTNG ) ( n = 55 , mean age 15 . 2 + / 4 . 5 years ) , in comparison with sex and age matched healthy control subjects ( n = 55 , mean age 15 . 2 + / 3 . 9 years ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The main co stimulatory pathway involves the interaction of CD 80 and CD 86 , expressed on the APCs , with their T cell counter receptor , CD 28 and CTLA 4 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
CD 80 cytoplasmic domain controls localization of CD 28 , CTLA 4 , and protein kinase Ctheta in the immunological synapse . ^^^ The binding of costimulatory ligand CD 80 to CD 28 or CTLA 4 on T cells plays an important role in the regulation of the T cell response . ^^^ In addition , we showed that CD 80 , CD 28 , and protein kinase Ctheta colocalized in the presence or absence of the CD 80 cytoplasmic tail . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Cytotoxic T lymphocyte antigen 4 ( CTLA 4 ; CD 152 ) is a member of the immunoglobulin gene superfamily with strong homology to the receptor CD 28 with which it shares the ligands CD 80 and CD 86 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Artificially enforced expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) on tumor cells renders them more immunogenic by triggering the CD 28 receptor on T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The results of surface plasma resonance show that binding between immobilized CD 28 and Cynarin is stronger than the binding between CD 28 and CD 80 , a co stimulated receptor of antigen presenting cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
When stimulated with beryllium / APC , the adhesion molecule ICAM 1 was up regulated , as well as several costimulation molecules including CD 28 , OX 40 ( CD 134 ) , 4 1 BB ( CD 137 ) and B 7 1 ( CD 80 ) . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The addition of agonistic Ab against CD 28 did not significantly increase proliferation or apoptosis of CIK cells redirected to CD 80 and CD 86 tumor targets . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
We generated bivalent CD 28 constructs by fusing the extracellular domains of CTLA 4 or CD 80 with the intracellular domains of CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Integration of CD 28 and CTLA 4 function results in differential responses of T cells to CD 80 and CD 86 . ^^^ CD 80 and CD 86 have the capacity to either stimulate or inhibit T cell responses through their receptors CD 28 and cytotoxic T lymphocyte associated antigen 4 ( CTLA 4 ) . ^^^ However , CD 80 committed a greater number of T cells to divide with faster kinetics , consistent with it being a superior ligand for CD 28 . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Signal two or co stimulation is mainly provided by the triggering of CD 28 on the T cell by CD 80 and CD 86 molecules on the DC . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
The interactions between CD 80 and CD 86 on antigen presenting cells and CD 28 on T cells serve as an important costimulatory signal in the activation of T cells . ^^^ Thus , CD 80 and CD 86 , signaling through CD 28 and possibly another unidentified receptor , are required for optimal immune surveillance and antiviral immune responses to murine gammaherpesvirus . . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
Although these two molecules bind the same ligand , CD 28 , the question of whether CD 80 and CD 86 provide unique signals or serve redundant roles remains controversial . ^^^ Previous studies have suggested that CD 80 binding to CD 28 may be superior to CD 86 for the activation of naive CD8+ T cells . ^^^
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA
Interacting proteins: P33681 and P10747	Pubmed	SVM Score :0.0
NA