| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.94247122 |
| RFX 5 was found to interact with CIITA , and this interaction was dependent on a proline rich domain within RFX 5 . 0.94247122^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.69762471 |
| We demonstrate direct interaction of CIITA with the MHC class 2 promoter binding protein RFX 5 and could also detect novel interactions with RFXANK , NF YB , and YC . 0.69762471^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| A genetic complementation cloning approach has led to the isolation of CIITA and RFX 5 , two essential MHC 2 gene transactivators . ^^^ CIITA and RFX 5 are mutated in these patients , and the wild type genes are capable of correcting their defect in MHC 2 expression . ^^^ Finally , the facts that CIITA and RFX 5 are both essential and highly specific for MHC 2 genes make possible novel strategies designed to achieve immunomodulation via transcriptional intervention . . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Specific complex formation between the type 2 bare lymphocyte syndrome associated transactivators CIITA and RFX 5 . ^^^ Transcriptional activation by the RFX 5 fusion protein could be restored in RJ2 . 2 . 5 by cotransfection with a CIITA expression plasmid . ^^^ Finally , a direct interaction between RFX 5 and CIITA was detected with the yeast two hybrid and far Western blot assays . ^^^ Thus , RFX 5 can activate transcription only in cooperation with CIITA . ^^^ RFX 5 and CIITA associate to form a complex capable of activating transcription from class 2 major histocompatibility complex promoters . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| METHODS : To elucidate the genetic defect in patients with MHC class 2 deficiency that was not classified genetically , we performed direct complementation assays with the three genes known to regulate the expression of MHC class 2 genes , CIITA , RFX 5 , and RFXAP , and the relevant mutations were identified in each patient . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| While the pivotal role of CIITA for MHC 2 gene transcription is supported by the essential absence of MHC 2 molecules in CIITA deficient mice , we demonstrate here that RFX 5 / mice retain expression of MHC 2 in thymic medulla , mature dendritic cells , and activated B cells . ^^^ This differential requirement for CIITA and RFX 5 in subsets of antigen presenting cells may be specific for the mouse ; it may , however , also exist in humans without having been noticed so far . . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| The factors defective in groups A ( CIITA ) , C ( RFX 5 ) and D ( RFXAP ) have been identified . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| The genetic defect leading to the bare lymphocyte syndrome was elucidated , too the mutations in genes coding transcription factors RFX 5 and CIITA are responsible for . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Despite the lack of functional RFX 5 and resulting MHC class 2 deficient phenotype , transfection of exogenous class 2 transactivator ( CIITA ) in these fibroblasts can overcome this defect , resulting in the expression of HLA DR , but not of DP , DQ , and invariant chain . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Unlike RFX 5 and RFXAP deficient cells , transfection of exogenous class 2 transactivator ( CIITA ) into these RFX B deficient fibroblasts resulted in the induction of HLA DR and HLA DP and , to a lesser extent , HLA DQ . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| First , 32 hematopoietic cell lines were analysed for the expression of HLA DR , CIITA , RFX 5 or HLA ABC . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Expression of major histocompatibility complex ( MHC ) class 2 genes is controlled at the transcriptional level by at least four trans acting genes , CIITA , RFXANK , RFX 5 , and RFXAP . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| In vivo analysis using both N terminal and C terminal deletion constructs identified critical domains of CIITA that are required for interaction with NF YB , NF YC , RFX 5 , RFXANK / RFXB , and CREB . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Mutations in cell surface receptors , signal transduction molecules and transcription factors have been described , including the common gamma chain of the IL 2 ( and IL 4 , IL 7 , IL 9 and IL 15 ) receptors , the kinase JAK 3 , the epsilon and gamma chains of CD 3 , the protein tyrosine kinase ZAP 70 , as well as CIITA and RFX 5 involved in MHC class 2 gene expression . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| BLS is the prototype of a `` disease of gene regulation . ' ' The affected genes encode RFXANK , RFX 5 , RFXAP , and CIITA , four regulatory factors that are highly specific and essential for MHCII genes . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| These genes encode CIITA , RFXANK , RFX 5 and RFXAP . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Four trans acting proteins control the coordinate expression of MHC class 2 molecules : class 2 trans activator ( CIITA ) , regulatory factor binding to the 10 box ( RFX ) associated protein ; RFX protein containing ankyrin repeats , and RFX 5 . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| PCR analysis of the HIV 1 infected cells that lost HLA DR expression revealed mRNA for W / Z / S ( RFX 5 ) , 10 1 ( RFX 5 ) , 10 2 ( hX 2BP ) , and one Y box DNA binding protein ( NF YB ) , and CIITA , a non DNA binding protein necessary for class 2 transcription . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| In addition to lack of CIITA expression , trophoblast cells also displayed a repressed expression of RFX 5 . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Four transacting genes , CIITA , RFXANK , RFX 5 , and RFXAP , control coordinate MHC 2 expression . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| RFX 5 complex activates MHC 2 transcription by interacting with an interferon gamma ( IFN gamma ) inducible protein , major histocompatibility class 2 trans activator ( CIITA ) . ^^^ IFN gamma enhances RFX 5 binding activity , and CIITA is present in the RFX 5 complex of IFN gamma treated human fibroblasts . ^^^ CIITA repressed collagen gene transcription more effectively in human fibroblasts than in rat fibroblasts , suggesting that the RFX 5 complex may , in part , recruit CIITA protein to the collagen transcription start site . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Major histocompatibility complex class 2 ( MHCII ) deficiency is a primary immunodeficiency resulting from defects in one of four different MHCII specific transcription factors CIITA , RFX 5 , RFXAP , and RFXANK . ^^^ This test is based on direct correction of the genetic defect by transduction of cells from patients with lentiviral vectors encoding CIITA , RFXANK , RFX 5 , or RFXAP . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| MHC class 2 deficiency is a combined immunodeficiency caused by defects in the four regulatory factors , CIITA , RFXANK , RFX 5 and RFXAP , that control MHC 2 expression at the transcriptional level . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Furthermore , using DNA affinity precipitation , we demonstrated that although CIITA binds at least five activators , RFX 5 , RFXAP , RFXANK / B , NFYB and NFYC , its assembly on the promoter requires the addition of nuclear extracts . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| CIITA does not bind DNA , but it interacts with the transcription factors RFX 5 , NF Y , and CREB and associated chromatin modifying enzymes to form an enhanceosome . ^^^ Inhibition of HDAC activity dramatically increases the association of NF YB and RFX 5 with CIITA , the assembly of CIITA , NF YB , and RFX 5 enhanceosome , and the extent of H 3 acetylation at the MHC 2 promoter . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Hyper phosphorylated CIITA interacts with co activator p 300 , RFX 5 and CIITA itself , which in turn results in induction of MHC class 2 promoter activity . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| However , others and we have recently described cases of mild or asymptomatic immunodeficiencies with defects in either CIITA ( class 2 transactivator ) or RFX 5 , both proteins required for the transcription of HLA D genes . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation assays , performed on HLA G positive and negative cell lines , demonstrated the in situ binding of RFX 5 and CIITA to HLA E and HLA F , but not to HLA G , promoters . ^^^ In B cells from bare lymphocyte syndrome patients lacking RFX 5 or CIITA , we observed lower steady state levels of HLA E and HLA F transcripts but did not find any significant decrease in the cell surface expression of HLA E / classical HLA class 1 . ^^^ RFX 5 and CIITA are thus not involved in HLA G expression and their importance for the surface expression of HLA E / classical HLA class 1 molecules may vary depending on the cell type . . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| These genes encode RFXANK , RFX 5 , RFXAP and CIITA . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Activation of MHC 2 by IFN gamma requires regulatory factor for 10 box 5 ( RFX 5 ) complex as well as class 2 transactivator ( CIITA ) . ^^^ RFX 5 recruits CIITA to the collagen site as evidenced by DNA affinity chromatography . ^^^ The presence of RFX 5 complex proteins enhances the collagen repression by CIITA reaching levels occurring during IFN gamma treatment . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Deficiencies in four proteins that regulate MHC 2 genes in humans ( RFX B , RFX 5 , RFXAP , and CIITA ) cause an inherited immunodeficiency disorder known as the bare lymphocyte syndrome ( BLS ) . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| In normal cells , RFX 5 complex along with major histocompatibility class 2 transactivator ( CIITA ) is induced by interferon gamma to occupy this site and repress collagen transcription ( Xu , Y . , Wang , L . , Buttice , G . , Sengupta , P . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| We demonstrate in a murine model of MHCII deficiency ( RFX 5 or CIITA deficient mice ) that residual MHCII expression by professional antigen presenting cells ( APCs ) is sufficient to support activation of adoptively transferred Th cells . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Previously , we demonstrated that major histocompatibility class 2 ( MHC 2 ) transactivator ( CIITA ) in a complex with regulatory factor for 10 box 5 ( RFX 5 ) is a crucial protein mediating interferon ( IFN ) gamma induced repression of collagen type 1 gene transcription in fibroblasts . ^^^ Levels of CIITA and RFX 5 increase in the nucleus of cells treated with IFN gamma . ^^^ This first demonstration that RFX 5 and CIITA isoforms are expressed in SMCs after IFN gamma stimulation suggest that CIITA could be a key factor in plaque stability in atherosclerosis . . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| Major histocompatibility complex class 2 deficiency , a rare autosomal recessive primary immunodeficiency , is caused by the defective expression of human leucocyte antigen ( HLA ) class 2 molecules due to mutated trans acting elements of any one of four regulatory genes ( CIITA , RFXANK , RFX 5 , RFXAP ) . ^^^ |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48382 and P33076 |
Pubmed |
SVM Score :0.0 |
| NA |
|