| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.64710235 |
| Mesenchymal cells also secrete CCL 19 , a ligand for CCR 7 , yet it is not clear whether CCR 7 and CCL 19 are important for secondary lymphoid organ development . 0.64710235^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.77369997 |
| Taken together , ELC is a highly specific ligand for EBI 1 , which is known to be expressed in activated B and T lymphocytes and strongly up regulated in B cells infected with Epstein Barr virus and T cells infected with herpesvirus 6 or 7 . 0.77369997^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we have identified CCR 4 as the specific receptor for TARC , GPR CY4 / DRY6 / CKR L3 / STRL22 as that for LARC ( CCR 6 ) , and EBI1 / BLR2 as that for ELC ( CCR 7 ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Here , we show that SLC is a high affinity functional ligand for chemokine receptor 7 ( CCR 7 ) that is expressed on T and B lymphocytes and a known receptor for ELC . ^^^ When assessed using CCR 7 transfected L1 . 2 cells , SLC and ELC were essentially equivalent in terms of cross desensitization in calcium mobilization via CCR 7 , cross competition in binding to CCR 7 , and induction of chemotaxis via CCR 7 . ^^^ SLC and ELC were also shown to fully share receptors expressed on cultured normal T cells known to express CCR 7 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Similar responses were obtained with EBI 1 ligand chemokine ( ELC ) , and sequential stimulation with both chemokines led to cross desensitization , suggesting that SLC acts via the ELC receptor , CCR 7 . ^^^ This was confirmed using murine pre B cells stably transfected with CCR 7 which bound SLC with high affinity and showed chemotaxis and Ca2+ mobilization in response to both SLC and ELC . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Like its human counterpart , murine ELC stimulated cells transfected with EBI 1 / CC chemokine receptor 7 ( CCR 7 ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We show that 6CK / SLC is an agonist for the lymphocyte chemoattractant receptor , CCR 7 ( EBI 1 , BLR 2 ) , previously described as a receptor for the related beta chemokine MIP 3beta ( ELC or Exodus 3 ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Concomitantly , the expression of CCR 7 and the migration to its ligand EBI 1 ligand chemokine ( ELC ) / macrophage inflammatory protein ( MIP ) 3beta , a chemokine expressed in lymphoid organs , were strongly up regulated , though with slower kinetics ( 24 48 h ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| While CCR 1 and CCR 5 were down regulated by endogenous or exogenous chemokines , CCR 7 levels gradually increased in maturing DC and showed a striking resistance to ligand induced down regulation , explaining how DC can sustain the response to SLC and ELC throughout the maturation process . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The ability of MIP 3 beta to augment IL 10 production correlated with CCR 7 mRNA expression and stimulation of intracellular calcium mobilization in both monocytes and T cells . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| This arrest is selectively inhibited by functional deletion ( desensitization ) of CC chemokine receptor 7 ( CCR 7 ) , the receptor for SLC and for macrophage inflammatory protein ( MIP ) 3beta ( EBV induced molecule 1 ligand chemokine [ ELC ] ) , and does not occur in mutant DDD / 1 mice that are deficient in these CCR 7 ligands . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| This observation is intriguing given that CCR 7 and its ligand EBI 1 Ligand Chemokine ( ELC ) , may play a role in the migration and homing of normal lymphocytes . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Stimulation of mature DCs with TGF beta 1 also enhanced TNF alpha induced down regulation of the expressions of CCR 1 , CCR 3 , CCR 5 , CCR 6 , and CXCR 4 , and chemotaxis to their respective ligands , while this stimulation suppressed TNF alpha induced expression of CCR 7 and chemotactic migratory ability to MIP 3 beta . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| These cells adhered to stalkokines representing ELC , a chemokine previously thought to bind only CCR 7 . ^^^ Adhesion was abolished in the presence of soluble ELC , SLC ( CCR 7 ligands ) , and TECK ( a CCR 9 ligand ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| EBI 1 ligand chemokine ( ELC ) attracts a broad spectrum of lymphocytes : activated T cells strongly up regulate CCR 7 and efficiently migrate toward ELC . ^^^ EBI 1 ligand chemokine ( ELC ) attracts a broad spectrum of lymphocytes : activated T cells strongly up regulate CCR 7 and efficiently migrate toward ELC . ^^^ Like ELC mRNA , CCR 7 mRNA was expressed in various lymphoid tissues . ^^^ By in situ hybridization , ELC and CCR 7 mRNA were detected in the parafollicular and inner cortical regions of a lymph node , and in the parafollicular regions of an appendix . ^^^ Collectively , ELC and CCR 7 may be involved in the trafficking of a broad spectrum of lymphocytes , especially activated T cells , into and within various lymphoid tissues . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Expression of CCR 7 , the receptor for MIP 3 beta , was detected at a mRNA level in the attracted CD34+ cells as well as input CD34+HPC . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Following stimulation with LPS or TNF alpha maturing DC expressed high levels of CCR 7 mRNA and acquired responsiveness to the CCR 7 ligand EBI 1 ligand chemokine ( ELC ) , a chemokine produced in lymphoid organs . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Cognate interaction of chemokine receptor CCR 7 on lymphocytes with its ligands CCL 19 and CCL 21 expressed on high endothelial venules ( HEVs ) is essential for effective migration of T and B cells across HEVs into secondary lymphoid organs . ^^^ Plt mice , which lack expression of CCL 19 and CCL 21 ser , both ligands for CCR 7 on HEVs , as well as CCR 7 deficient mice , have a defective cell migration and reduced homing of lymphocytes . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Another CC chemokine CCL 21 , which shares CCR 7 with CCL 19 but has a unique C terminal extension containing positively charged amino acids , failed to show involvement in thymic emigration . ^^^ Neonatal appearance of circulating T cells was defective in CCL 19 neutralized mice as well as in CCR 7 deficient mice but not in CCL 21 neutralized mice . ^^^ These results indicate a CCL 19 and CCR 7 dependent pathway of thymic emigration , which represents a major pathway of neonatal T cell export . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| In contrast to other chemokines , such as monocyte chemotactic protein 1 ( CC chemokine receptor 2 [ CCR 2 ] ) , macrophage inflammatory protein 1beta ( CCR 5 ) , liver and activation regulated chemokine ( LARC [ CCR 6 ] ) , Epstein Barr virus induced molecule 1 ligand chemokine ( ELC [ CCR 7 ] ) , and IP 10 ( CXCR 3 ) , SDF 1 stimulates the prolonged activation of protein kinase B and extracellular signal regulated kinase ( ERK ) 2 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The CCR 7 ligand elc ( CCL 19 ) is transcytosed in high endothelial venules and mediates T cell recruitment . ^^^ We conclude that perivascular ELC is transcytosed to the luminal surfaces of HEVs and participates in CCR 7 mediated triggering of lymphocyte arrest . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The respective roles of the CCR 7 ligands CCL 19 and CCL 21 in mediating migration are not fully defined , but chemotaxis to CCL 19 mediates Langerhans cell exit from the epidermis . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Chemotaxis assays showed that SDF 1 , which binds CXCR 4 ; MDC , which binds CCR 4 ; and ELC , which binds CCR 7 , mediated significant chemotaxis of thymocytes . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The CCR 7 ligands , secondary lymphoid tissue chemokine ( SLC ) and Epstein Barr virus induced molecule 1 ligand chemokine ( ELC ) , were recently recognized as key molecules in establishing functional microenvironments for the initiation of immune responses in secondary lymphoid tissue . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The T cell chemokine receptor CCR 7 is internalized on stimulation with ELC , but not with SLC . ^^^ ELC and SLC are potent agonists for CCR 7 , a receptor of up most importance for the regulation of the homing and traffic of lymphocytes into and within secondary lymphoid tissues . ^^^ ELC induced a rapid time and concentration dependent internalization of CCR 7 and markedly decreased the ability of CCR 7 bearing cells to respond to a second stimulation . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Using LC type cells generated in vitro in the presence of transforming growth factor ( TGF ) beta 1 , we further found that tumor necrosis factor ( TNF ) alpha , as a prototype proinflammatory factor , and a variety of inflammatory stimuli and bacterial products , increase Langerin expression and Langerin dependent Birbeck granules formation in cell which nevertheless lack costimulatory molecules , DC LAMP / CD208 and potent T cell stimulatory activity but express CCR 7 and respond to the lymph node homing chemokines CCL 19 and CCL 21 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the CD 4 ( + ) CD 25 ( + ) CD62L ( + ) splenocytes express CCR 7 at high levels and migrate toward secondary lymphoid tissue chemokine and ELC ( macrophage inflammatory protein 3beta ) , lymphoid chemokines , whereas CD 4 ( + ) CD 25 ( + ) CD62L ( ) splenocytes preferentially express CCR 2 , CCR 4 , and CXCR 3 and migrate toward the corresponding inflammatory chemokines . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Here we show that antigen engaged B cells have increased expression of CCR 7 , the receptor for the T zone chemokines CCL 19 and CCL 21 , and that they exhibit increased responsiveness to both chemoattractants . ^^^ In mice lacking lymphoid CCL 19 and CCL 21 chemokines , or with B cells that lack CCR 7 , antigen engagement fails to cause movement to the T zone . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the expression of CCR 7 , the receptor for both CCL 21 and CCL 19 , correlated with clinical lymphadenopathy , and blocking of CCR 7 inhibited CLL cell TEM . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Our results document that activation of human naive T cells and differentiation in Th 1 or Th 2 cells result in progressive down modulation of CCR 7 expression and CCL 19 responsiveness . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 7 and its ligands , CCL 19 and CCL 21 , are involved in lymphocyte recirculation through secondary lymphoid organs and additionally navigate lymphocytes into distinct tissue compartments . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Salmonella exposed DCs as well as mature DCs , but not immature DCs , were recruited towards the CC chemokines CCL 19 and CCL 21 , two ligands of CCR 7 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Moreover , activation of PPAR gamma in DC inhibited the expression of EBI 1 ligand chemokine and CCR 7 , both playing a pivotal role for DC migration to the lymph nodes . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 7 expression , responsiveness to CCL 19 , and L selectin / alpha ( 4 ) beta ( 7 ) phenotype are coordinately regulated . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| In the present study , using a murine spleen derived DC line , we analyzed effects of CCR 7 ligands , CCL 19 and CCL 21 , on dendritic morphology . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The lymphocyte expressed chemokine receptor CCR 7 is playing an important role during this process , as its HEV presented ligands CCL 19 and CCL 21 can trigger rapid integrin activation under flow in addition to inducing a chemotactic response , which may participate in transmigration and / or interstitial migration . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 7 binds CCL 19 and CCL 21 , causing the highly activated DCs to be trapped in the peripheral tissue site . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| To analyse the nature of T cell mature DC interactions further in chronically inflamed skin and LNs , the present study focuses on another chemokine system , namely CCL 19 ( EBI 1 ligand chemokine ; ELC ) , CCL 21 ( secondary lymphoid tissue chemokine ; SLC ) and their shared receptor , CCR 7 . ^^^ RT PCR analysis revealed expression of CCL 19 , CCL 21 , and CCR 7 at high levels in LNs and at low levels in inflamed skin . ^^^ Using immunohistochemistry , the majority of DC Lamp ( + ) mature DCs in the T cell area of LNs expressed CCL 19 and were surrounded by CCR 7 ( + ) nave type lymphocytes , while CCL 21 was expressed in reticular stromal cells and vascular endothelial cells . ^^^ In contrast , the majority of DC Lamp ( + ) mature DCs in inflamed skin were totally negative for CCL 19 and were surrounded by CCR 7 ( ) memory type T cells . ^^^ In normal skin , on the other hand , no cells immunoreactive for CCL 19 , CCL 21 , or CCR 7 were found . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Transcripts for CCL 19 and CCL 21 and their common receptor CCR 7 were induced in the CNS of mice undergoing relapsing remitting and chronic relapsing EAE . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| In the present study , we examined the effects of CCL 19 and CCL 21 , 2 CCR 7 ligands , on endocytosis of fluorescein isothiocyanate ( FITC ) dextran by murine DCs . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| ATG were shown to bind to CXCR 4 and CCR 7 on lymphocytes , CXCR 4 , and CCR 5 on monocytes ; to down modulate cell surface expression of CCR 7 ; and to decrease monocyte chemotactic response to CCL 5 ( RANTES ) and lymphocyte chemotactic response to CCL 19 ( MIP 3beta ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Within 24h of local antigen priming , the expression of three chemokine receptor genes ( CCR 3 , CCR 5 , and CCR 7 ) and three chemokine ligand genes ( CCL 12 , CCL 19 , and CCL 25 ) were significantly upregulated . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Interestingly , a second CCR 7 ligand , CCL 19 , triggered T cell sticking in cremaster muscle venules , but failed to induce extravasation in air pouches . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We studied the role of Rho kinase and extracellular signal regulated kinase ( ERK ) 2 in the polarization and migration of T lymphocytes in response to the CCR 7 ligands EBI 1 ligand chemokine ( ELC ; CCL 19 ) and secondary lymphoid tissue chemokine ( SLC ; CCL 21 ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Homeostatic chemokines , such as CXCL 13 , CCL 21 , and CCL 19 , as well as their corresponding receptors , CXCR 5 and CCR 7 , have now been shown to closely cooperate in the development of lymphoid organs and the maintenance of lymphoid tissue microarchitecture . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND AND AIMS : The chemokines CCL 19 and CCL 21 bind CCR 7 , which is involved in the organization of secondary lymphoid tissue and is expressed during chronic tissue inflammation . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Activated DCs home to lymphoid organs , where the CCR 7 ligands CCL 19 and CCL 21 are expressed . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCL 19 and CCR 7 expressing cells were absent in both tissues . ^^^ Detection of mature DCs in DM and PM muscle tissue samples despite the lack of CCL 19 and CCR 7 is evidence for a local maturation of DCs in inflammatory muscle tissue without lymphoid organ organization . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 7 and its ligands CCL 19 and CCL 21 have been identified as the gatekeepers for both nave T lymphocytes and dendritic cells ( DC ) to these defined anatomical compartments . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 7 , the receptor for the medullary chemokines CCL 19 and CCL 21 , is induced on thymocytes during their positive selection . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| In this study we investigated the early signaling events of CCR 7 initiated by its two endogenous ligands , CCL 19 and CCL 21 . ^^^ However , only activation by CCL 19 , not CCL 21 , promotes robust desensitization of endogenous CCR 7 in the human T cell lymphoma cell line H 9 . ^^^ The sites of CCL 19 induced phosphorylation were mapped by mutating to alanines the serines and threonines found within kinase phosphorylation consensus sequences in the carboxyl terminus of CCR 7 . ^^^ Taken together , these findings demonstrate that CCL 19 and CCL 21 place CCR 7 in functionally distinct conformations that are independent of their G protein coupling potency : one that allows the efficient desensitization of the receptor and activation of ERK1 / 2 , and another that is impaired in these functions . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Furthermore , MS loaded mature MoDC expressed normal levels of the chemokine receptor CCR 7 and migrated as efficiently towards CCL 19 or CCL 21 as unloaded MoDC . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| To investigate CCL 19 mediated modulations of cellular immune responses in vivo , we generated a chimeric CCL 19 immunoglobulin G2b ( IgG2b ) Fc fusion protein , which binds to the chemokine receptor CCR 7 comparable to native CCL 19 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Recent studies have implicated the ligands CCL 19 and CCL 21 and their receptor , CCR 7 , in the specific migration of nave lymphocytes and mature dendritic cells to secondary lymphoid organs during immune homeostasis . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We found both in skin lesions and in invaded lymph nodes an expression by tumor cells of CXCR 3 , CXCR 4 , and CCR 7 , and the concomitant expression by cells in the microenvironment of their respective ligands CXCL 9 , CXCL 12 , and CCL 19 . ^^^ IL 3 or virus induced activation of LPDC leads to downregulation of CXCR 3 and CXCR 4 , and upregulation of CCR 7 , associated with the loss of response to CXCL 12 , and the acquisition of sensitivity to CCL 19 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Both haptens were able to induce CCR 7 expression and DC migration in response to Cys Cys chemokine ligand , CCL 19 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Following lipopolysaccharide ( LPS ) stimulation , the dendritic cells down regulated CCR 1 , 2 , and 5 expression , up regulated or sustained signals for CCR 7 , and migrated to the constitutively expressed ligands CCL 19 ( MIP 3beta ) and CCL 21 ( secondary lymphoid tissue chemokine ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Despite these changes in the IFN DC migratory behavior , the expression of CCR 5 and CCR 7 and the calcium fluxes triggered by CCL 4 and CCL 19 were not affected . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| BCR / ABL mediated downregulation of genes implicated in cell adhesion and motility leads to impaired migration toward CCR 7 ligands CCL 19 and CCL 21 in primary BCR / ABL positive cells . ^^^ Compared with GFP UCB or normal ( NL ) BM CD34+ cells , p 210 UCB and CML CD34+ cells migrated poorly towards the CCR 7 ligands , CCL 19 and CCL 21 , suggesting a possible role for CCR 7 in the abnormal migratory behavior of CML CD34+ cells . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Using the Millipore MultiScreen MIC and FLIPR assays , alanine substitutions at K 130 and Q 227 caused threefold shifts in potency for the CCR 7 ligand , CCL 19 , whereas that at K 137 had no effect . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| A minority of BM MSCs ( 2 % to 25 % ) expressed a restricted set of chemokine receptors ( CXC receptor 4 [ CXCR 4 ] , CX3C receptor 1 [ CX3CR1 ] , CXCR 6 , CC chemokine receptor 1 [ CCR 1 ] , CCR 7 ) and , accordingly , showed appreciable chemotactic migration in response to the chemokines CXC ligand 12 ( CXCL 12 ) , CX3CL1 , CXCL 16 , CC chemokine ligand 3 ( CCL 3 ) , and CCL 19 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Moreover , mature PG / DC fail to up regulate the chemokine receptor CCR 7 and show an impaired migration towards its ligand CCL 19 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Importantly , only activated DCs that migrated from the periphery to draining lymph nodes , but not resting steady state DCs residing within lymph nodes , expressed high levels of CCR 7 in vivo and responded to CCL 19 with the production of proinflammatory cytokines . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| This contact is the result of highly coordinated migration processes , in which the chemokine receptor CCR 7 and its ligands , CCL 19 and CCL 21 , play a central role . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Furthermore , migration assays revealed that , upon infection of mature DCs , CCR 7 and CXCR 4 mediated migration towards the corresponding CCL 19 and CXCL 12 chemokine gradients was strongly reduced . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| In addition , treatment of CCR 7 ( + ) metastatic SCCHN cells with CCL 19 ( MIP 3beta ) showed rapid activation of the prosurvival , PI3K / Akt pathway . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 7 ligands , CCL 19 and CCL 21 , induce not only chemotaxis but also endocytosis in mature DCs . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 7 expression induced by HIV 1 was sufficient to initiate migration of DCs in the presence of secondary lymphoid tissue chemokine ( CCL 21 ) and MIP 3beta ( CCL 19 ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We also show that the migration of type 1 diabetes monocytes towards the lymphoid tissue related CCL 19 was increased and correlated with an increased CCR 7 surface expression on the monocytes . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Freshly isolated CCR 7 ( + ) early pre B ALL cells migrated to CCL 19 , whereas CCR 7 ( + ) pro B and pre B ALL cells were attracted by CCL 19 only following culture with soluble recombinant CD 40 ligand . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of CCL 19 , CCL 20 , and CCL 21 as well as their receptors CCR 6 ( for CCL 20 ) and CCR 7 ( for CCL 19 and CCL 21 ) was characterised by immunohistochemistry and , in addition , their cellular localisation was determined by double immunofluorescence investigations . ^^^ Increased expression of the CCR 7 ligands CCL 19 by DC themselves as well as CCL 21 by reticular cells and lymphatic vessels was observed in Crohn ' s disease , thereby causing the matured DC to be trapped at the site of inflammation . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CD 3 , CD 83 , HECA 452 , VEGFR 3 , SLC , ELC , and CCR 7 expression was studied immunohistochemically . ^^^ SLC , ELC , and CCR 7 mRNA was significantly higher in CD MLNs compared with UC . ^^^ CONCLUSION : The dominant Th 1 immune response is facilitated by interaction of SLC positive HEVs / lymphatic vessels , ELC positive mature DCs , and CCR 7 positive T cells in hyperplastic T cell areas . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Transendothelial migration of FTY 720 P treated immature DCs to the CCR 7 ligand CCL 19 was reduced . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We found that CXCL 13 and CCL 19 together by means of activation of CXCR 5 and CCR 7 up regulated PEG 10 expression and function , subsequent stabilized caspase 3 and caspase 8 in B ALL and B CLL CD19+CD34+ B cells , and rescued the cells from TNF alpha mediated apoptosis . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Although LPS up regulates CCR 7 on DCs , a second signal is required to enable them to migrate toward the chemokine CCL 19 ( MIP 3beta ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We engrafted fully allogeneic islets into streptozotocin treated normal mice or mice with the autosomal recessive paucity of lymph node T cell ( plt ) mutation ; the latter lack secondary lymphoid expression of the CCR 7 ligands , secondary lymphoid organ chemokine ( CCL 21 ) and EBV induced molecule 1 ligand chemokine ( CCL 19 ) . plt mice showed permanent survival of islets engrafted under the kidney capsule , whereas controls rejected islet allografts in 12 days ( p < 0 . 001 ) , and consistent with this , plt mice had normal allogeneic T cell responses , but deficient migration of donor dendritic cell to draining lymph nodes . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| This study shows that ZAP 70 ( + ) CLL B cells respond in vitro more readily than ZAP 70 ( ) CLL and normal B cells to chemokine migratory signals through enhanced surface CCR 7 expression ( P = . 009 ; P < . 001 ) and increased responsiveness to its ligands CCL 19 and CCL 21 , demonstrated by F actin polymerization ( P < . 05 ) and cellular migration ( P < . 01 ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Transgenic thymocytes demonstrated unimpaired lymphoid organ entry in adoptive transfer studies but demonstrated impaired thymic exodus in response to CCL 19 , apparently independent of CCR 7 , S1P1 , and NF kappaB . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| METHODS : The expression of two chemokine receptors , CCR 7 and CXCR 4 , and their respective ligands , CCL 19 , CCL 21 , and CXCL 12 , were examined in surgical lung biopsies ( SLBs ) from patients with IIP . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND / PURPOSE : Chemokine receptor ( CCR 7 [ cysteine chemokine receptor 7 ] ) and ligand ( CCL 19 ) interactions trigger dendritic cell ( DC ) recruitment from sites of antigen uptake to secondary lymphoid organs for T cell priming and tumor lysis . ^^^ CCR 7 and CCL 19 protein and RNA expressions by control and NB exposed DC were measured by flow cytometry , Western blot analysis , and polymerase chain reaction . ^^^ RESULTS : No significant differences in CCR 7 or CCL 19 protein expression between tumor and control were seen at 7 days . ^^^ CONCLUSIONS : Although in vitro coculture with NB does not induce significant changes in either CCR 7 or CCL 19 expression , profound functional impairments in CCR7 / CCL19 mediated migration occurs . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| By comparing the impact of chemokine receptor engagement on mature DC we found that the CCR 7 ligand CCL 19 holds a stronger chemotactic potency than the CXCR 4 ligand CXCL 12 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Dendritic cells express CCR 7 and migrate in response to CCL 19 ( MIP 3beta ) after exposure to Helicobacter pylori . ^^^ Migration of DC to lymph nodes is regulated by chemokine receptor CCR 7 , expressed on mature DC , and the CCR 7 ligands CCL 19 and CCL 21 . ^^^ Despite low surface expression of CCR 7 protein following stimulation with H . pylori compared to E . coli , the DC migrated equally well towards CCL 19 after stimulation with both bacteria . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Remarkably , when CCR 7 function was abrogated in vivo by treatment of WT recipients with antibodies to CCR 7 ligands CCL 19 and CCL 21 , lesion size and foam cell content were substantially preserved . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Homing of lymphocytes from blood into secondary lymphoid tissues beyond high endothelial venules is highly dependent on the interaction between the chemokines CCL 19 , CCL 21 , CXCL 12 , and CXCL 13 , and their receptors CCR 7 , CXCR 4 and CXCR 5 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Moreover , these mAb blocked the in vitro migration of CLL cells in response to CC chemokine ligand 19 ( CCL 19 ) , one of the physiological ligands of CCR 7 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Finally , labeled CCL 19 was used for the staining of primary cells , illustrating that this reagent can be used for studying CCR 7 expression on different cell types . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| We provide evidence that the migration pattern of transferred BDC and BDC Idd 9 T cells at least partly depends on their differential chemotaxis toward the CCR 7 ligand CCL 19 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| In vitro , CD 47 ( / ) mature DCs showed normal CCR 7 expression but impaired migration to CCL 19 , whereas immature DC response to CCL 5 was only slightly impaired . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Dendritic cells ( DCs ) acquire , during their maturation , the expression of the chemokine receptor CCR 7 and the ability to migrate to lymph nodes in response to CC chemokine ligand 19 ( CCL 19 ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The expression of CCR 7 by these cells may be induced by the hepatic microenvironment to facilitate recirculation . 3 ) The CCR 7 ligands CCL 19 and CCL 21 are present on lymphatic , vascular , and sinusoidal endothelium in normal liver and in patients with HCV infection . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The chemokines CCL 19 , CCL 21 and CCL 25 , by signalling through the receptors CCR 7 or CCR 9 , play critical roles in leukocyte homing . ^^^ Here , using transfected HEK 293 cells , we have shown that both CCX CKR and CCR 7 mediate rapid CCL 19 internalisation upon initial chemokine exposure . ^^^ More importantly , CCR 7 rapidly became refractory for CCL 19 uptake , but the sequestration activity of CCX CKR was enhanced . ^^^ These properties endowed CCX CKR with an impressive ability to mediate progressive sequestration and degradation of large quantities of CCL 19 , and conversely , prevented CCR 7 expressing cells from extensively altering their chemokine environment . ^^^ However , over expression of caveolin 1 , which stabilises caveolae , blocked CCL 19 uptake by CCX CKR while having no impact on other chemokine receptors , including CCR 7 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| In MLNs , DCs bearing the unique LP DC phenotype ( CD11chighCD8alphaintCD11blowalphaLlowbeta7high and CD11chighCD8alpha CD11bhighalphaLlowbeta7high ) were abundant in wild type mice , but were markedly fewer in CCL 19 , CCL 21 Ser deficient plt / plt mice and were almost absent in CCR 7 deficient mice , indicating the critical importance of CCR 7 in LP DC trafficking to MLNs . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The chemokine receptor CCR 7 and its ligands CCL 19 and CCL 21 play a crucial role for the homing of lymphocytes and dendritic cells to secondary lymphoid tissues . ^^^ In this study , we demonstrate that CCR 7 ( GFP ) is endocytosed into early endosomes containing transferrin receptor upon CCL 19 binding , but less upon CCL 21 triggering . ^^^ In contrast , internalized CCL 19 was sorted to lysosomes for degradation , showing opposite fate for endocytosed CCR 7 and its ligand . . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CC chemokine receptor 7 ( CCR 7 ) , which regulates the trafficking of leucocytes to the secondary lymphoid organs , has two endogenous chemokine ligands : CCL 19 and CCL 21 . ^^^ Here , we show that substituting N terminal six amino acids of CCL 21 ( SDGGAQ ) for the corresponding N terminal domain of CCL 19 ( GTNDAE ) results in a chimeric chemokine that exhibits high affinity binding and G protein activation of CCR 7 . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CCR 2 , CCR 5 , CCR 7 , CCL 19 , and interleukin 12a were upregulated in a time and tissue dependent manner . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : Dexamethazone ( DEX ) and IL 10 inhibited human DC migration to CCL 19 in vitro and mouse DC migration to lymph nodes ( LN ) in vivo , by impairing CCR 7 expression . ^^^ Rapamycin ( RAPA ) increased DC migration to CCL 19 in vitro and to LN in vivo by enhancing CCR 7 expression . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| At the same time , the CXCR 5 ( hi ) cells showed reduced responsiveness to the T zone chemokines , Epstein Barr virus induced molecule 1 ( EBI 1 ) ligand chemokine ( ELC ) and secondary lymphoid tissue chemokine ( SLC ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Gene duplications at the chemokine locus on mouse chromosome 4 : multiple strain specific haplotypes and the deletion of secondary lymphoid organ chemokine and EBI 1 ligand chemokine genes in the plt mutation . ^^^ We now find that the plt defect is due to the loss of both SLC and EBI 1 ligand chemokine ( ELC ) expression in secondary lymphoid organs . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| CD83+ mature DC became responsive to EBI 1 ligand chemokine ( ELC ) , a chemokine specifically expressed in secondary lymphoid organs . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , we found that for all maturation stimuli tested , the addition of prostaglandin E 2 ( PGE 2 ) was required for effective migration of MoDCs toward the lymph node derived chemokines CCL 19 ( EBI 1 ligand chemokine / macrophage inflammatory protein 3beta ) and CCL 21 ( secondary lymphoid tissue chemokine [ SLC ] / 6Ckine ) . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| The expression levels of B lymphocyte chemoattractant , EBI 1 ligand chemokine , and stromal cell derived factor 1alpha mRNAs were up regulated in cocultured human B cells and in infected human gastric mucosa . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| MIP 3 beta protein was associated predominantly with alveolar macrophages and correlated with BALF lymphocytes and T cell subsets . mRNA expression for the MIP 3 beta receptor , CC chemokine receptor 7 , was increased in sarcoidosis and correlated with MIP 3 beta protein levels . ^^^ |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P32248 and Q99731 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.64710235 |
| Mesenchymal cells also secrete CCL 19 , a ligand for CCR 7 , yet it is not clear whether CCR 7 and CCL 19 are important for secondary lymphoid organ development . 0.64710235^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.77369997 |
| Taken together , ELC is a highly specific ligand for EBI 1 , which is known to be expressed in activated B and T lymphocytes and strongly up regulated in B cells infected with Epstein Barr virus and T cells infected with herpesvirus 6 or 7 . 0.77369997^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The CCR 7 ligand elc ( CCL 19 ) is transcytosed in high endothelial venules and mediates T cell recruitment . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The respective roles of the CCR 7 ligands CCL 19 and CCL 21 in mediating migration are not fully defined , but chemotaxis to CCL 19 mediates Langerhans cell exit from the epidermis . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Cognate interaction of chemokine receptor CCR 7 on lymphocytes with its ligands CCL 19 and CCL 21 expressed on high endothelial venules ( HEVs ) is essential for effective migration of T and B cells across HEVs into secondary lymphoid organs . ^^^ Plt mice , which lack expression of CCL 19 and CCL 21 ser , both ligands for CCR 7 on HEVs , as well as CCR 7 deficient mice , have a defective cell migration and reduced homing of lymphocytes . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Another CC chemokine CCL 21 , which shares CCR 7 with CCL 19 but has a unique C terminal extension containing positively charged amino acids , failed to show involvement in thymic emigration . ^^^ Neonatal appearance of circulating T cells was defective in CCL 19 neutralized mice as well as in CCR 7 deficient mice but not in CCL 21 neutralized mice . ^^^ These results indicate a CCL 19 and CCR 7 dependent pathway of thymic emigration , which represents a major pathway of neonatal T cell export . . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Here we show that antigen engaged B cells have increased expression of CCR 7 , the receptor for the T zone chemokines CCL 19 and CCL 21 , and that they exhibit increased responsiveness to both chemoattractants . ^^^ In mice lacking lymphoid CCL 19 and CCL 21 chemokines , or with B cells that lack CCR 7 , antigen engagement fails to cause movement to the T zone . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the expression of CCR 7 , the receptor for both CCL 21 and CCL 19 , correlated with clinical lymphadenopathy , and blocking of CCR 7 inhibited CLL cell TEM . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Our results document that activation of human naive T cells and differentiation in Th 1 or Th 2 cells result in progressive down modulation of CCR 7 expression and CCL 19 responsiveness . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 7 and its ligands , CCL 19 and CCL 21 , are involved in lymphocyte recirculation through secondary lymphoid organs and additionally navigate lymphocytes into distinct tissue compartments . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Salmonella exposed DCs as well as mature DCs , but not immature DCs , were recruited towards the CC chemokines CCL 19 and CCL 21 , two ligands of CCR 7 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , we found that for all maturation stimuli tested , the addition of prostaglandin E 2 ( PGE 2 ) was required for effective migration of MoDCs toward the lymph node derived chemokines CCL 19 ( EBI 1 ligand chemokine / macrophage inflammatory protein 3beta ) and CCL 21 ( secondary lymphoid tissue chemokine [ SLC ] / 6Ckine ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 7 expression , responsiveness to CCL 19 , and L selectin / alpha ( 4 ) beta ( 7 ) phenotype are coordinately regulated . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Using LC type cells generated in vitro in the presence of transforming growth factor ( TGF ) beta 1 , we further found that tumor necrosis factor ( TNF ) alpha , as a prototype proinflammatory factor , and a variety of inflammatory stimuli and bacterial products , increase Langerin expression and Langerin dependent Birbeck granules formation in cell which nevertheless lack costimulatory molecules , DC LAMP / CD208 and potent T cell stimulatory activity but express CCR 7 and respond to the lymph node homing chemokines CCL 19 and CCL 21 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| In the present study , using a murine spleen derived DC line , we analyzed effects of CCR 7 ligands , CCL 19 and CCL 21 , on dendritic morphology . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The lymphocyte expressed chemokine receptor CCR 7 is playing an important role during this process , as its HEV presented ligands CCL 19 and CCL 21 can trigger rapid integrin activation under flow in addition to inducing a chemotactic response , which may participate in transmigration and / or interstitial migration . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 7 binds CCL 19 and CCL 21 , causing the highly activated DCs to be trapped in the peripheral tissue site . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| To analyse the nature of T cell mature DC interactions further in chronically inflamed skin and LNs , the present study focuses on another chemokine system , namely CCL 19 ( EBI 1 ligand chemokine ; ELC ) , CCL 21 ( secondary lymphoid tissue chemokine ; SLC ) and their shared receptor , CCR 7 . ^^^ RT PCR analysis revealed expression of CCL 19 , CCL 21 , and CCR 7 at high levels in LNs and at low levels in inflamed skin . ^^^ Using immunohistochemistry , the majority of DC Lamp ( + ) mature DCs in the T cell area of LNs expressed CCL 19 and were surrounded by CCR 7 ( + ) nave type lymphocytes , while CCL 21 was expressed in reticular stromal cells and vascular endothelial cells . ^^^ In contrast , the majority of DC Lamp ( + ) mature DCs in inflamed skin were totally negative for CCL 19 and were surrounded by CCR 7 ( ) memory type T cells . ^^^ In normal skin , on the other hand , no cells immunoreactive for CCL 19 , CCL 21 , or CCR 7 were found . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Transcripts for CCL 19 and CCL 21 and their common receptor CCR 7 were induced in the CNS of mice undergoing relapsing remitting and chronic relapsing EAE . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| In the present study , we examined the effects of CCL 19 and CCL 21 , 2 CCR 7 ligands , on endocytosis of fluorescein isothiocyanate ( FITC ) dextran by murine DCs . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| ATG were shown to bind to CXCR 4 and CCR 7 on lymphocytes , CXCR 4 , and CCR 5 on monocytes ; to down modulate cell surface expression of CCR 7 ; and to decrease monocyte chemotactic response to CCL 5 ( RANTES ) and lymphocyte chemotactic response to CCL 19 ( MIP 3beta ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Within 24h of local antigen priming , the expression of three chemokine receptor genes ( CCR 3 , CCR 5 , and CCR 7 ) and three chemokine ligand genes ( CCL 12 , CCL 19 , and CCL 25 ) were significantly upregulated . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Interestingly , a second CCR 7 ligand , CCL 19 , triggered T cell sticking in cremaster muscle venules , but failed to induce extravasation in air pouches . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We studied the role of Rho kinase and extracellular signal regulated kinase ( ERK ) 2 in the polarization and migration of T lymphocytes in response to the CCR 7 ligands EBI 1 ligand chemokine ( ELC ; CCL 19 ) and secondary lymphoid tissue chemokine ( SLC ; CCL 21 ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Homeostatic chemokines , such as CXCL 13 , CCL 21 , and CCL 19 , as well as their corresponding receptors , CXCR 5 and CCR 7 , have now been shown to closely cooperate in the development of lymphoid organs and the maintenance of lymphoid tissue microarchitecture . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND AND AIMS : The chemokines CCL 19 and CCL 21 bind CCR 7 , which is involved in the organization of secondary lymphoid tissue and is expressed during chronic tissue inflammation . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Activated DCs home to lymphoid organs , where the CCR 7 ligands CCL 19 and CCL 21 are expressed . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCL 19 and CCR 7 expressing cells were absent in both tissues . ^^^ Detection of mature DCs in DM and PM muscle tissue samples despite the lack of CCL 19 and CCR 7 is evidence for a local maturation of DCs in inflammatory muscle tissue without lymphoid organ organization . . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 7 and its ligands CCL 19 and CCL 21 have been identified as the gatekeepers for both nave T lymphocytes and dendritic cells ( DC ) to these defined anatomical compartments . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 7 , the receptor for the medullary chemokines CCL 19 and CCL 21 , is induced on thymocytes during their positive selection . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| In this study we investigated the early signaling events of CCR 7 initiated by its two endogenous ligands , CCL 19 and CCL 21 . ^^^ However , only activation by CCL 19 , not CCL 21 , promotes robust desensitization of endogenous CCR 7 in the human T cell lymphoma cell line H 9 . ^^^ The sites of CCL 19 induced phosphorylation were mapped by mutating to alanines the serines and threonines found within kinase phosphorylation consensus sequences in the carboxyl terminus of CCR 7 . ^^^ Taken together , these findings demonstrate that CCL 19 and CCL 21 place CCR 7 in functionally distinct conformations that are independent of their G protein coupling potency : one that allows the efficient desensitization of the receptor and activation of ERK1 / 2 , and another that is impaired in these functions . . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Furthermore , MS loaded mature MoDC expressed normal levels of the chemokine receptor CCR 7 and migrated as efficiently towards CCL 19 or CCL 21 as unloaded MoDC . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| To investigate CCL 19 mediated modulations of cellular immune responses in vivo , we generated a chimeric CCL 19 immunoglobulin G2b ( IgG2b ) Fc fusion protein , which binds to the chemokine receptor CCR 7 comparable to native CCL 19 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Recent studies have implicated the ligands CCL 19 and CCL 21 and their receptor , CCR 7 , in the specific migration of nave lymphocytes and mature dendritic cells to secondary lymphoid organs during immune homeostasis . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We found both in skin lesions and in invaded lymph nodes an expression by tumor cells of CXCR 3 , CXCR 4 , and CCR 7 , and the concomitant expression by cells in the microenvironment of their respective ligands CXCL 9 , CXCL 12 , and CCL 19 . ^^^ IL 3 or virus induced activation of LPDC leads to downregulation of CXCR 3 and CXCR 4 , and upregulation of CCR 7 , associated with the loss of response to CXCL 12 , and the acquisition of sensitivity to CCL 19 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Both haptens were able to induce CCR 7 expression and DC migration in response to Cys Cys chemokine ligand , CCL 19 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Following lipopolysaccharide ( LPS ) stimulation , the dendritic cells down regulated CCR 1 , 2 , and 5 expression , up regulated or sustained signals for CCR 7 , and migrated to the constitutively expressed ligands CCL 19 ( MIP 3beta ) and CCL 21 ( secondary lymphoid tissue chemokine ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 7 expression induced by HIV 1 was sufficient to initiate migration of DCs in the presence of secondary lymphoid tissue chemokine ( CCL 21 ) and MIP 3beta ( CCL 19 ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Despite these changes in the IFN DC migratory behavior , the expression of CCR 5 and CCR 7 and the calcium fluxes triggered by CCL 4 and CCL 19 were not affected . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| BCR / ABL mediated downregulation of genes implicated in cell adhesion and motility leads to impaired migration toward CCR 7 ligands CCL 19 and CCL 21 in primary BCR / ABL positive cells . ^^^ Compared with GFP UCB or normal ( NL ) BM CD34+ cells , p 210 UCB and CML CD34+ cells migrated poorly towards the CCR 7 ligands , CCL 19 and CCL 21 , suggesting a possible role for CCR 7 in the abnormal migratory behavior of CML CD34+ cells . . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Using the Millipore MultiScreen MIC and FLIPR assays , alanine substitutions at K 130 and Q 227 caused threefold shifts in potency for the CCR 7 ligand , CCL 19 , whereas that at K 137 had no effect . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| A minority of BM MSCs ( 2 % to 25 % ) expressed a restricted set of chemokine receptors ( CXC receptor 4 [ CXCR 4 ] , CX3C receptor 1 [ CX3CR1 ] , CXCR 6 , CC chemokine receptor 1 [ CCR 1 ] , CCR 7 ) and , accordingly , showed appreciable chemotactic migration in response to the chemokines CXC ligand 12 ( CXCL 12 ) , CX3CL1 , CXCL 16 , CC chemokine ligand 3 ( CCL 3 ) , and CCL 19 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Moreover , mature PG / DC fail to up regulate the chemokine receptor CCR 7 and show an impaired migration towards its ligand CCL 19 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Importantly , only activated DCs that migrated from the periphery to draining lymph nodes , but not resting steady state DCs residing within lymph nodes , expressed high levels of CCR 7 in vivo and responded to CCL 19 with the production of proinflammatory cytokines . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| This contact is the result of highly coordinated migration processes , in which the chemokine receptor CCR 7 and its ligands , CCL 19 and CCL 21 , play a central role . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Furthermore , migration assays revealed that , upon infection of mature DCs , CCR 7 and CXCR 4 mediated migration towards the corresponding CCL 19 and CXCL 12 chemokine gradients was strongly reduced . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| In addition , treatment of CCR 7 ( + ) metastatic SCCHN cells with CCL 19 ( MIP 3beta ) showed rapid activation of the prosurvival , PI3K / Akt pathway . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 7 ligands , CCL 19 and CCL 21 , induce not only chemotaxis but also endocytosis in mature DCs . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We also show that the migration of type 1 diabetes monocytes towards the lymphoid tissue related CCL 19 was increased and correlated with an increased CCR 7 surface expression on the monocytes . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Freshly isolated CCR 7 ( + ) early pre B ALL cells migrated to CCL 19 , whereas CCR 7 ( + ) pro B and pre B ALL cells were attracted by CCL 19 only following culture with soluble recombinant CD 40 ligand . . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of CCL 19 , CCL 20 , and CCL 21 as well as their receptors CCR 6 ( for CCL 20 ) and CCR 7 ( for CCL 19 and CCL 21 ) was characterised by immunohistochemistry and , in addition , their cellular localisation was determined by double immunofluorescence investigations . ^^^ Increased expression of the CCR 7 ligands CCL 19 by DC themselves as well as CCL 21 by reticular cells and lymphatic vessels was observed in Crohn ' s disease , thereby causing the matured DC to be trapped at the site of inflammation . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Transendothelial migration of FTY 720 P treated immature DCs to the CCR 7 ligand CCL 19 was reduced . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We found that CXCL 13 and CCL 19 together by means of activation of CXCR 5 and CCR 7 up regulated PEG 10 expression and function , subsequent stabilized caspase 3 and caspase 8 in B ALL and B CLL CD19+CD34+ B cells , and rescued the cells from TNF alpha mediated apoptosis . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Although LPS up regulates CCR 7 on DCs , a second signal is required to enable them to migrate toward the chemokine CCL 19 ( MIP 3beta ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We engrafted fully allogeneic islets into streptozotocin treated normal mice or mice with the autosomal recessive paucity of lymph node T cell ( plt ) mutation ; the latter lack secondary lymphoid expression of the CCR 7 ligands , secondary lymphoid organ chemokine ( CCL 21 ) and EBV induced molecule 1 ligand chemokine ( CCL 19 ) . plt mice showed permanent survival of islets engrafted under the kidney capsule , whereas controls rejected islet allografts in 12 days ( p < 0 . 001 ) , and consistent with this , plt mice had normal allogeneic T cell responses , but deficient migration of donor dendritic cell to draining lymph nodes . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| This study shows that ZAP 70 ( + ) CLL B cells respond in vitro more readily than ZAP 70 ( ) CLL and normal B cells to chemokine migratory signals through enhanced surface CCR 7 expression ( P = . 009 ; P < . 001 ) and increased responsiveness to its ligands CCL 19 and CCL 21 , demonstrated by F actin polymerization ( P < . 05 ) and cellular migration ( P < . 01 ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Transgenic thymocytes demonstrated unimpaired lymphoid organ entry in adoptive transfer studies but demonstrated impaired thymic exodus in response to CCL 19 , apparently independent of CCR 7 , S1P1 , and NF kappaB . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| METHODS : The expression of two chemokine receptors , CCR 7 and CXCR 4 , and their respective ligands , CCL 19 , CCL 21 , and CXCL 12 , were examined in surgical lung biopsies ( SLBs ) from patients with IIP . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND / PURPOSE : Chemokine receptor ( CCR 7 [ cysteine chemokine receptor 7 ] ) and ligand ( CCL 19 ) interactions trigger dendritic cell ( DC ) recruitment from sites of antigen uptake to secondary lymphoid organs for T cell priming and tumor lysis . ^^^ CCR 7 and CCL 19 protein and RNA expressions by control and NB exposed DC were measured by flow cytometry , Western blot analysis , and polymerase chain reaction . ^^^ RESULTS : No significant differences in CCR 7 or CCL 19 protein expression between tumor and control were seen at 7 days . ^^^ CONCLUSIONS : Although in vitro coculture with NB does not induce significant changes in either CCR 7 or CCL 19 expression , profound functional impairments in CCR7 / CCL19 mediated migration occurs . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| By comparing the impact of chemokine receptor engagement on mature DC we found that the CCR 7 ligand CCL 19 holds a stronger chemotactic potency than the CXCR 4 ligand CXCL 12 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Dendritic cells express CCR 7 and migrate in response to CCL 19 ( MIP 3beta ) after exposure to Helicobacter pylori . ^^^ Migration of DC to lymph nodes is regulated by chemokine receptor CCR 7 , expressed on mature DC , and the CCR 7 ligands CCL 19 and CCL 21 . ^^^ Despite low surface expression of CCR 7 protein following stimulation with H . pylori compared to E . coli , the DC migrated equally well towards CCL 19 after stimulation with both bacteria . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Remarkably , when CCR 7 function was abrogated in vivo by treatment of WT recipients with antibodies to CCR 7 ligands CCL 19 and CCL 21 , lesion size and foam cell content were substantially preserved . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Homing of lymphocytes from blood into secondary lymphoid tissues beyond high endothelial venules is highly dependent on the interaction between the chemokines CCL 19 , CCL 21 , CXCL 12 , and CXCL 13 , and their receptors CCR 7 , CXCR 4 and CXCR 5 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Moreover , these mAb blocked the in vitro migration of CLL cells in response to CC chemokine ligand 19 ( CCL 19 ) , one of the physiological ligands of CCR 7 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Finally , labeled CCL 19 was used for the staining of primary cells , illustrating that this reagent can be used for studying CCR 7 expression on different cell types . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We provide evidence that the migration pattern of transferred BDC and BDC Idd 9 T cells at least partly depends on their differential chemotaxis toward the CCR 7 ligand CCL 19 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| In vitro , CD 47 ( / ) mature DCs showed normal CCR 7 expression but impaired migration to CCL 19 , whereas immature DC response to CCL 5 was only slightly impaired . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Dendritic cells ( DCs ) acquire , during their maturation , the expression of the chemokine receptor CCR 7 and the ability to migrate to lymph nodes in response to CC chemokine ligand 19 ( CCL 19 ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The expression of CCR 7 by these cells may be induced by the hepatic microenvironment to facilitate recirculation . 3 ) The CCR 7 ligands CCL 19 and CCL 21 are present on lymphatic , vascular , and sinusoidal endothelium in normal liver and in patients with HCV infection . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The chemokines CCL 19 , CCL 21 and CCL 25 , by signalling through the receptors CCR 7 or CCR 9 , play critical roles in leukocyte homing . ^^^ Here , using transfected HEK 293 cells , we have shown that both CCX CKR and CCR 7 mediate rapid CCL 19 internalisation upon initial chemokine exposure . ^^^ More importantly , CCR 7 rapidly became refractory for CCL 19 uptake , but the sequestration activity of CCX CKR was enhanced . ^^^ These properties endowed CCX CKR with an impressive ability to mediate progressive sequestration and degradation of large quantities of CCL 19 , and conversely , prevented CCR 7 expressing cells from extensively altering their chemokine environment . ^^^ However , over expression of caveolin 1 , which stabilises caveolae , blocked CCL 19 uptake by CCX CKR while having no impact on other chemokine receptors , including CCR 7 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| In MLNs , DCs bearing the unique LP DC phenotype ( CD11chighCD8alphaintCD11blowalphaLlowbeta7high and CD11chighCD8alpha CD11bhighalphaLlowbeta7high ) were abundant in wild type mice , but were markedly fewer in CCL 19 , CCL 21 Ser deficient plt / plt mice and were almost absent in CCR 7 deficient mice , indicating the critical importance of CCR 7 in LP DC trafficking to MLNs . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The chemokine receptor CCR 7 and its ligands CCL 19 and CCL 21 play a crucial role for the homing of lymphocytes and dendritic cells to secondary lymphoid tissues . ^^^ In this study , we demonstrate that CCR 7 ( GFP ) is endocytosed into early endosomes containing transferrin receptor upon CCL 19 binding , but less upon CCL 21 triggering . ^^^ In contrast , internalized CCL 19 was sorted to lysosomes for degradation , showing opposite fate for endocytosed CCR 7 and its ligand . . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CC chemokine receptor 7 ( CCR 7 ) , which regulates the trafficking of leucocytes to the secondary lymphoid organs , has two endogenous chemokine ligands : CCL 19 and CCL 21 . ^^^ Here , we show that substituting N terminal six amino acids of CCL 21 ( SDGGAQ ) for the corresponding N terminal domain of CCL 19 ( GTNDAE ) results in a chimeric chemokine that exhibits high affinity binding and G protein activation of CCR 7 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CCR 2 , CCR 5 , CCR 7 , CCL 19 , and interleukin 12a were upregulated in a time and tissue dependent manner . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : Dexamethazone ( DEX ) and IL 10 inhibited human DC migration to CCL 19 in vitro and mouse DC migration to lymph nodes ( LN ) in vivo , by impairing CCR 7 expression . ^^^ Rapamycin ( RAPA ) increased DC migration to CCL 19 in vitro and to LN in vivo by enhancing CCR 7 expression . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Similar responses were obtained with EBI 1 ligand chemokine ( ELC ) , and sequential stimulation with both chemokines led to cross desensitization , suggesting that SLC acts via the ELC receptor , CCR 7 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Concomitantly , the expression of CCR 7 and the migration to its ligand EBI 1 ligand chemokine ( ELC ) / macrophage inflammatory protein ( MIP ) 3beta , a chemokine expressed in lymphoid organs , were strongly up regulated , though with slower kinetics ( 24 48 h ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| EBI 1 ligand chemokine ( ELC ) attracts a broad spectrum of lymphocytes : activated T cells strongly up regulate CCR 7 and efficiently migrate toward ELC . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Following stimulation with LPS or TNF alpha maturing DC expressed high levels of CCR 7 mRNA and acquired responsiveness to the CCR 7 ligand EBI 1 ligand chemokine ( ELC ) , a chemokine produced in lymphoid organs . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| This observation is intriguing given that CCR 7 and its ligand EBI 1 Ligand Chemokine ( ELC ) , may play a role in the migration and homing of normal lymphocytes . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Gene duplications at the chemokine locus on mouse chromosome 4 : multiple strain specific haplotypes and the deletion of secondary lymphoid organ chemokine and EBI 1 ligand chemokine genes in the plt mutation . ^^^ We now find that the plt defect is due to the loss of both SLC and EBI 1 ligand chemokine ( ELC ) expression in secondary lymphoid organs . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| CD83+ mature DC became responsive to EBI 1 ligand chemokine ( ELC ) , a chemokine specifically expressed in secondary lymphoid organs . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Moreover , activation of PPAR gamma in DC inhibited the expression of EBI 1 ligand chemokine and CCR 7 , both playing a pivotal role for DC migration to the lymph nodes . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Augmented expression of secondary lymphoid tissue chemokine and EBI 1 ligand chemokine in Crohn ' s disease . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The expression levels of B lymphocyte chemoattractant , EBI 1 ligand chemokine , and stromal cell derived factor 1alpha mRNAs were up regulated in cocultured human B cells and in infected human gastric mucosa . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we have identified CCR 4 as the specific receptor for TARC , GPR CY4 / DRY6 / CKR L3 / STRL22 as that for LARC ( CCR 6 ) , and EBI1 / BLR2 as that for ELC ( CCR 7 ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Here , we show that SLC is a high affinity functional ligand for chemokine receptor 7 ( CCR 7 ) that is expressed on T and B lymphocytes and a known receptor for ELC . ^^^ When assessed using CCR 7 transfected L1 . 2 cells , SLC and ELC were essentially equivalent in terms of cross desensitization in calcium mobilization via CCR 7 , cross competition in binding to CCR 7 , and induction of chemotaxis via CCR 7 . ^^^ SLC and ELC were also shown to fully share receptors expressed on cultured normal T cells known to express CCR 7 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We show that 6CK / SLC is an agonist for the lymphocyte chemoattractant receptor , CCR 7 ( EBI 1 , BLR 2 ) , previously described as a receptor for the related beta chemokine MIP 3beta ( ELC or Exodus 3 ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Like its human counterpart , murine ELC stimulated cells transfected with EBI 1 / CC chemokine receptor 7 ( CCR 7 ) . ^^^ Epstein Barr virus induced molecule 1 ( EBI 1 ) ligand chemokine ( ELC / MIP3beta ) is expressed constitutively within lymphoid tissues and may act as such a guidance cue . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| While CCR 1 and CCR 5 were down regulated by endogenous or exogenous chemokines , CCR 7 levels gradually increased in maturing DC and showed a striking resistance to ligand induced down regulation , explaining how DC can sustain the response to SLC and ELC throughout the maturation process . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| At the same time , the CXCR 5 ( hi ) cells showed reduced responsiveness to the T zone chemokines , Epstein Barr virus induced molecule 1 ( EBI 1 ) ligand chemokine ( ELC ) and secondary lymphoid tissue chemokine ( SLC ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| This arrest is selectively inhibited by functional deletion ( desensitization ) of CC chemokine receptor 7 ( CCR 7 ) , the receptor for SLC and for macrophage inflammatory protein ( MIP ) 3beta ( EBV induced molecule 1 ligand chemokine [ ELC ] ) , and does not occur in mutant DDD / 1 mice that are deficient in these CCR 7 ligands . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| These cells adhered to stalkokines representing ELC , a chemokine previously thought to bind only CCR 7 . ^^^ Adhesion was abolished in the presence of soluble ELC , SLC ( CCR 7 ligands ) , and TECK ( a CCR 9 ligand ) . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| In contrast to other chemokines , such as monocyte chemotactic protein 1 ( CC chemokine receptor 2 [ CCR 2 ] ) , macrophage inflammatory protein 1beta ( CCR 5 ) , liver and activation regulated chemokine ( LARC [ CCR 6 ] ) , Epstein Barr virus induced molecule 1 ligand chemokine ( ELC [ CCR 7 ] ) , and IP 10 ( CXCR 3 ) , SDF 1 stimulates the prolonged activation of protein kinase B and extracellular signal regulated kinase ( ERK ) 2 . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Chemotaxis assays showed that SDF 1 , which binds CXCR 4 ; MDC , which binds CCR 4 ; and ELC , which binds CCR 7 , mediated significant chemotaxis of thymocytes . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The CCR 7 ligands , secondary lymphoid tissue chemokine ( SLC ) and Epstein Barr virus induced molecule 1 ligand chemokine ( ELC ) , were recently recognized as key molecules in establishing functional microenvironments for the initiation of immune responses in secondary lymphoid tissue . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The T cell chemokine receptor CCR 7 is internalized on stimulation with ELC , but not with SLC . ^^^ ELC and SLC are potent agonists for CCR 7 , a receptor of up most importance for the regulation of the homing and traffic of lymphocytes into and within secondary lymphoid tissues . ^^^ ELC induced a rapid time and concentration dependent internalization of CCR 7 and markedly decreased the ability of CCR 7 bearing cells to respond to a second stimulation . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the CD 4 ( + ) CD 25 ( + ) CD62L ( + ) splenocytes express CCR 7 at high levels and migrate toward secondary lymphoid tissue chemokine and ELC ( macrophage inflammatory protein 3beta ) , lymphoid chemokines , whereas CD 4 ( + ) CD 25 ( + ) CD62L ( ) splenocytes preferentially express CCR 2 , CCR 4 , and CXCR 3 and migrate toward the corresponding inflammatory chemokines . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Expression of CCR 7 , the receptor for MIP 3 beta , was detected at a mRNA level in the attracted CD34+ cells as well as input CD34+HPC . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| The ability of MIP 3 beta to augment IL 10 production correlated with CCR 7 mRNA expression and stimulation of intracellular calcium mobilization in both monocytes and T cells . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| Stimulation of mature DCs with TGF beta 1 also enhanced TNF alpha induced down regulation of the expressions of CCR 1 , CCR 3 , CCR 5 , CCR 6 , and CXCR 4 , and chemotaxis to their respective ligands , while this stimulation suppressed TNF alpha induced expression of CCR 7 and chemotactic migratory ability to MIP 3 beta . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| MIP 3 beta protein was associated predominantly with alveolar macrophages and correlated with BALF lymphocytes and T cell subsets . mRNA expression for the MIP 3 beta receptor , CC chemokine receptor 7 , was increased in sarcoidosis and correlated with MIP 3 beta protein levels . ^^^ |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99731 and P32248 |
Pubmed |
SVM Score :0.0 |
| NA |
|