Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.51154443
Although the mechanisms are unknown , we find that the chemokine CCL 21 ( also known as TCA 4 , SLC , 6Ckine ) , a ligand for the chemokine receptor CCR 7 , can regulate homeostasis of CD 4 ( but not CD 8 ) T cells . 0.51154443^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We show that 6CK / SLC is an agonist for the lymphocyte chemoattractant receptor , CCR 7 ( EBI 1 , BLR 2 ) , previously described as a receptor for the related beta chemokine MIP 3beta ( ELC or Exodus 3 ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Secondary lymphoid tissue chemokine is a functional ligand for the CC chemokine receptor CCR 7 . ^^^ Here , we show that SLC is a high affinity functional ligand for chemokine receptor 7 ( CCR 7 ) that is expressed on T and B lymphocytes and a known receptor for ELC . ^^^ SLC induced a vigorous calcium mobilization in murine L1 . 2 cells stably expressing human CCR 7 . ^^^ SLC tagged with the secreted form of alkaline phosphatase ( SLC SEAP ) showed specific binding to CCR 7 that was fully competed by SLC with an IC 50 of 0 . 5 nM . ^^^ SLC also induced a vigorous chemotactic response in CCR 7 expressing L1 . 2 cells with a typical bell shaped dose response curve and a maximal migration at 10 nM . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR 7 . ^^^ Similar responses were obtained with EBI 1 ligand chemokine ( ELC ) , and sequential stimulation with both chemokines led to cross desensitization , suggesting that SLC acts via the ELC receptor , CCR 7 . ^^^ This was confirmed using murine pre B cells stably transfected with CCR 7 which bound SLC with high affinity and showed chemotaxis and Ca2+ mobilization in response to both SLC and ELC . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Cutting edge : secondary lymphoid tissue chemokine ( SLC ) and CC chemokine receptor 7 ( CCR 7 ) participate in the emigration pathway of mature dendritic cells from the skin to regional lymph nodes . ^^^ Secondary lymphoid tissue chemokine ( SLC ) , a CC chemokine , is expressed in secondary lymphoid organs and mediates the chemotaxis of lymphocytes and DCs via its receptor , CC chemokine receptor 7 ( CCR 7 ) . ^^^ Secondary lymphoid tissue chemokine ( SLC ) , a CC chemokine , is expressed in secondary lymphoid organs and mediates the chemotaxis of lymphocytes and DCs via its receptor , CC chemokine receptor 7 ( CCR 7 ) . ^^^ Thus , we provide direct evidence that SLC and CCR 7 participate in the emigration of DCs from peripheral tissue to LNs via lymphatics . . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In agreement with the chemokine receptor mRNA expression by each population in the DC differentiation pathway , SLC ( also termed as MIP 3beta ) , one of the ligands for CCR 7 , strongly and selectively chemoattracted both CD11b / dullCD11c+ and CD11b+hiCD11c+ DC precursors ( days 6 to 7 ) and more mature DCs ( days 13 to 14 ) . ^^^ We observed here that TGF beta 1 not only inhibited the expression of CCR 7 in DCs and DC precursors derived from HPCs , but also inhibited the migration of these cells in response to SLC . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
While CCR 1 and CCR 5 were down regulated by endogenous or exogenous chemokines , CCR 7 levels gradually increased in maturing DC and showed a striking resistance to ligand induced down regulation , explaining how DC can sustain the response to SLC and ELC throughout the maturation process . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In contrast , mature DC have lost their responsiveness to most of these chemokines through receptor down regulation or desensitization , but acquired responsiveness to MIP 3beta / ELC and 6Ckine / SLC as a consequence of CCR 7 up regulation . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The appearance of these responses is associated with the phenotypic stage of cortex to medulla migration and with up regulation of mRNA for the receptors CCR 4 ( for macrophage derived chemokine and thymus and activation regulated chemokine ) and CCR 7 ( for secondary lymphoid tissue chemokine and macrophage inflammatory protein 3beta ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The CC chemokine known as 6Ckine ( SLC , Exodus 2 , or TCA 4 ) has been identified as a ligand for CCR 7 . ^^^ In this study , we report that human 6Ckine does not induce a calcium flux in either human CXCR 3 or mouse CXCR 3 transfected cells , although it is an equally potent agonist as mouse 6Ckine and human macrophage inflammatory protein 3beta in human CCR 7 transfected cells . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Using a novel transwell chemotaxis assay system , we demonstrate that the CC chemokine receptor 7 ( CCR 7 ) ligands 6Ckine and macrophage inflammatory protein ( MIP ) 3 beta are selective chemoattractants for MHC class IIhigh B 7 2high bone marrow derived dendritic cells at a potency 1000 fold higher than their known activity on naive T cells . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 ligands , SLC / 6Ckine / Exodus2 / TCA4 and CKbeta 11 / MIP 3beta / ELC , are chemoattractants for CD 56 ( + ) CD 16 ( ) NK cells and late stage lymphoid progenitors . ^^^ SLC and CKbeta 11 share only 32 % amino acid identity , but are ligands for the same chemokine receptor , CCR 7 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In particular , immature DC express CC chemokine receptor 5 ( CCR 5 ) and migrate in response to macrophage inflammatory protein 1alpha ( MIP 1alpha ) , whereas mature DC switch expression to CCR 7 and respond exclusively to MIP 3beta and 6Ckine . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Adhesion was abolished in the presence of soluble ELC , SLC ( CCR 7 ligands ) , and TECK ( a CCR 9 ligand ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
SLC / exodus2 / 6Ckine / TCA4 induces chemotaxis of hematopoietic progenitor cells : differential activity of ligands of CCR 7 , CXCR 3 , or CXCR 4 in chemotaxis vs . suppression of progenitor proliferation . ^^^ We examined the chemotactic activity of CXCR 3 ligands on CD34+ HPC because it has been reported that SLC is a potential ligand of CXC chemokine receptor , CXCR 3 , in addition to a CC chemokine receptor , CCR 7 . ^^^ In this regard , CCR 7 ligands ( SLC and CKbeta 11 ) , but not IP 10 and MIG , induce actin polymerization in CD34+ cells . ^^^ Our results suggest that SLC is a potential trafficking factor for HPC , and that chemokines that bind CCR 7 , CXCR 4 , and CXCR 3 have differential biological activities on HPC in terms of suppression and chemotaxis . . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Pharmacological and signaling analysis of human chemokine receptor CCR 7 stably expressed in HEK 293 cells : high affinity binding of recombinant ligands MIP 3beta and SLC stimulates multiple signaling cascades . ^^^ Thorough analyses of the pharmacological profiles of the recombinant ligands for CCR 7 , MIP 3beta / ELC / CK beta 11 , and SLC / Exodus 2 / TCA4 / 6C kine , using CCR 7 expressing HEK 293E transfectants determine that ligands both bind with a K ( d ) in the 100 pM range 10 to 100 fold greater affinities than published K ( d ) values . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In contrast , no significant effect on IL 10 production was observed when 6Ckine , the other reported ligand for human CCR 7 , or other CC chemokines such as monocyte chemoattractant protein 1 , RANTES , MIP 1 alpha , and MIP 1 beta were added to LPS or PHA stimulated PBMCs . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The different homing patterns of naive and effector CD 8 T cells in vivo correlated well with their CCR 7 chemokine receptor expression and their reactivity to the secondary lymphoid tissue chemokine ( SLC ) . ^^^ Thus , down regulation of CCR 7 expression on CD 8 effector T cells rendered them unre sponsive to SLC , which controls T cell homing into white pulp of spleen and lymph nodes . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Desensitization of the TCA 4 receptor , CC chemokine receptor 7 ( CCR 7 ) , blocked T ( GFP ) cell adherence in wild type HEVs , whereas desensitization to stromal cell derived factor ( SDF ) 1alpha ( the ligand for CXC chemokine receptor 4 [ CXCR 4 ] ) did not affect T ( GFP ) cell behavior . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
This arrest is selectively inhibited by functional deletion ( desensitization ) of CC chemokine receptor 7 ( CCR 7 ) , the receptor for SLC and for macrophage inflammatory protein ( MIP ) 3beta ( EBV induced molecule 1 ligand chemokine [ ELC ] ) , and does not occur in mutant DDD / 1 mice that are deficient in these CCR 7 ligands . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Migration of dendritic cells from the skin to lymph nodes is absolutely required for induction of hapten sensitization , and depends upon expression of CCR 7 by mature dendritic cells and SLC in the lymph nodes . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Chemotaxis assay showed that CCR 7 ligands , macrophage inflammatory protein 3beta and secondary lymphoid tissue chemokine significantly ( P < 0 . 05 ) induced the migration of DCs when cocultured with apoptotic tumor cells . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
During the extrafollicular response , plasmablast expression of CXCR 5 and responsiveness to B lymphocyte chemoattractant ( CXCR 5 ) as well as to secondary lymphoid tissue chemokine ( CCR 7 ) and stromal cell derived factor ( SDF ) 1 ( CXCR 4 ) are lost while a weak response towards the CCR 6 chemokine LARC is maintained . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Since mRNA encoding CXCR 3 , an alternative receptor for SLC , but no CCR 7 mRNA was found in microglia , we suggest that the effects of SLC on microglia are mediated by CXCR 3 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The respective roles of the CCR 7 ligands CCL 19 and CCL 21 in mediating migration are not fully defined , but chemotaxis to CCL 19 mediates Langerhans cell exit from the epidermis . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The CCR 7 ligands , secondary lymphoid tissue chemokine ( SLC ) and Epstein Barr virus induced molecule 1 ligand chemokine ( ELC ) , were recently recognized as key molecules in establishing functional microenvironments for the initiation of immune responses in secondary lymphoid tissue . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The functional assay for CCR 7 protein was Ca ( 2+ ) flux stimulated by the chemokine CCL 21 , a CCR 7 specific ligand produced by lymphatic endothelial cells . ^^^ Lymph node metastasis of CCR 7 B16 cells was blocked by neutralizing anti CCL 21 antibodies ( metastasis in none of five lymph nodes ) but not by control immunoglobulin G ( three of five ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The T cell chemokine receptor CCR 7 is internalized on stimulation with ELC , but not with SLC . ^^^ ELC and SLC are potent agonists for CCR 7 , a receptor of up most importance for the regulation of the homing and traffic of lymphocytes into and within secondary lymphoid tissues . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Cognate interaction of chemokine receptor CCR 7 on lymphocytes with its ligands CCL 19 and CCL 21 expressed on high endothelial venules ( HEVs ) is essential for effective migration of T and B cells across HEVs into secondary lymphoid organs . ^^^ Plt mice , which lack expression of CCL 19 and CCL 21 ser , both ligands for CCR 7 on HEVs , as well as CCR 7 deficient mice , have a defective cell migration and reduced homing of lymphocytes . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Another CC chemokine CCL 21 , which shares CCR 7 with CCL 19 but has a unique C terminal extension containing positively charged amino acids , failed to show involvement in thymic emigration . ^^^ Neonatal appearance of circulating T cells was defective in CCL 19 neutralized mice as well as in CCR 7 deficient mice but not in CCL 21 neutralized mice . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Here we show that antigen engaged B cells have increased expression of CCR 7 , the receptor for the T zone chemokines CCL 19 and CCL 21 , and that they exhibit increased responsiveness to both chemoattractants . ^^^ In mice lacking lymphoid CCL 19 and CCL 21 chemokines , or with B cells that lack CCR 7 , antigen engagement fails to cause movement to the T zone . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In microglial cells cultured from CCR 7 knockout mice , CCL 21 produced the same type of Cl ( ) current as well as a chemotaxis response . ^^^ We conclude that the CCL 21 induced Cl ( ) current is a prerequisite for the chemotaxis response mediated by the activation of CXCR 3 but not CCR 7 receptors , indicating that in brain CCL 21 acts via a different receptor system than in lymphoid organs . . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Furthermore , the expression of CCR 7 , the receptor for both CCL 21 and CCL 19 , correlated with clinical lymphadenopathy , and blocking of CCR 7 inhibited CLL cell TEM . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
They up regulated the expression of HLA DR , CD 80 , CD 86 , partially CCR 7 but not CD 83 , partially reduced antigen uptake function , increased the levels of IL 12p35 mRNA , and prolonged surface expression of peptide MHC class 1 complexes for presentation to cytotoxic T lymphocytes , but did not induce migration towards CCL 21 chemokine . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Intrahepatic lymphocytes in PSC include a population of CCR 7 ( + ) T cells only half of which express CD45RA and which respond to CCL 21 in migration assays . ^^^ The expression of CCL 21 in association with mucosal addressin cell adhesion molecule 1 in portal tracts in PSC may promote the recruitment and retention of CCR 7 ( + ) mucosal lymphocytes leading to the establishment of chronic portal inflammation and the expanded portal associated lymphoid tissue . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 and its ligands , CCL 19 and CCL 21 , are involved in lymphocyte recirculation through secondary lymphoid organs and additionally navigate lymphocytes into distinct tissue compartments . ^^^ Chemotactic reactivity of these cells toward CCL 21 was absent in CCR 7 deficient cells , proving that effector cells do not use alternative receptors for this chemokine . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Salmonella exposed DCs as well as mature DCs , but not immature DCs , were recruited towards the CC chemokines CCL 19 and CCL 21 , two ligands of CCR 7 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
All these effects were reduced following in vivo neutralization of the CCR 7 ligand secondary lymphoid tissue chemokine . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Using LC type cells generated in vitro in the presence of transforming growth factor ( TGF ) beta 1 , we further found that tumor necrosis factor ( TNF ) alpha , as a prototype proinflammatory factor , and a variety of inflammatory stimuli and bacterial products , increase Langerin expression and Langerin dependent Birbeck granules formation in cell which nevertheless lack costimulatory molecules , DC LAMP / CD208 and potent T cell stimulatory activity but express CCR 7 and respond to the lymph node homing chemokines CCL 19 and CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We demonstrate that the CD 4 ( + ) CD 25 ( + ) CD62L ( + ) splenocytes express CCR 7 at high levels and migrate toward secondary lymphoid tissue chemokine and ELC ( macrophage inflammatory protein 3beta ) , lymphoid chemokines , whereas CD 4 ( + ) CD 25 ( + ) CD62L ( ) splenocytes preferentially express CCR 2 , CCR 4 , and CXCR 3 and migrate toward the corresponding inflammatory chemokines . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In the present study , using a murine spleen derived DC line , we analyzed effects of CCR 7 ligands , CCL 19 and CCL 21 , on dendritic morphology . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Encephalitogenic T cells showed surface expression of CCR 7 and the alternative receptor for CCL 21 , CXCR 3 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
These cells expressed higher levels of alpha ( 4 ) beta ( 7 ) integrins than other gammadelta T cell subsets and selectively migrated to the CCR 7 ligand secondary lymphoid tissue chemokine ( CCL 21 ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The receptor for CCL 21 , CCR 7 , was also found to be highly expressed on the infiltrating T cells , most of which expressed the memory CD45Ro phenotype . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The lymphocyte expressed chemokine receptor CCR 7 is playing an important role during this process , as its HEV presented ligands CCL 19 and CCL 21 can trigger rapid integrin activation under flow in addition to inducing a chemotactic response , which may participate in transmigration and / or interstitial migration . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
SIV infection altered the expression of chemokines ( CCL19 / MIP 3beta , CCL21 / 6Ckine , and CCL20 / MIP 3alpha ) and of chemokine receptors ( CCR 7 and CCR 6 ) that drive DC trafficking . ^^^ CCL19 / MIP 3beta , CCL20 / MIP 3alpha , CCR 6 , and CCR 7 expression increased in lymph nodes during the early systemic burst of viral replication ( acute infection ) , whereas CCL21 / 6Ckine expression progressively decreased throughout disease to AIDS . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 binds CCL 19 and CCL 21 , causing the highly activated DCs to be trapped in the peripheral tissue site . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
To analyse the nature of T cell mature DC interactions further in chronically inflamed skin and LNs , the present study focuses on another chemokine system , namely CCL 19 ( EBI 1 ligand chemokine ; ELC ) , CCL 21 ( secondary lymphoid tissue chemokine ; SLC ) and their shared receptor , CCR 7 . ^^^ RT PCR analysis revealed expression of CCL 19 , CCL 21 , and CCR 7 at high levels in LNs and at low levels in inflamed skin . ^^^ Using immunohistochemistry , the majority of DC Lamp ( + ) mature DCs in the T cell area of LNs expressed CCL 19 and were surrounded by CCR 7 ( + ) nave type lymphocytes , while CCL 21 was expressed in reticular stromal cells and vascular endothelial cells . ^^^ Furthermore , CCL 21 expression in the inflamed skin was detected in dermal lymphatic endothelial cells and rare CCR 7 ( + ) mature DCs were mostly seen within the lymphatic vessels . ^^^ In normal skin , on the other hand , no cells immunoreactive for CCL 19 , CCL 21 , or CCR 7 were found . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In the present article , we show that maturing Mtb infected DC express high levels of CCR 7 and they become responsive to its ligand CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In LAG 3 matured DC , surface expression of CCR 5 ( a receptor for MIP 1alpha / CCL3 ) was down regulated and CCR 7 ( a receptor for MIP 3beta and SLC ) was up regulated . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Upon contact with KpOmpA , human immature DC and macrophages acquire CCR 7 expression and responsiveness to CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Transcripts for CCL 19 and CCL 21 and their common receptor CCR 7 were induced in the CNS of mice undergoing relapsing remitting and chronic relapsing EAE . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In the present study , we examined the effects of CCL 19 and CCL 21 , 2 CCR 7 ligands , on endocytosis of fluorescein isothiocyanate ( FITC ) dextran by murine DCs . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We studied the role of Rho kinase and extracellular signal regulated kinase ( ERK ) 2 in the polarization and migration of T lymphocytes in response to the CCR 7 ligands EBI 1 ligand chemokine ( ELC ; CCL 19 ) and secondary lymphoid tissue chemokine ( SLC ; CCL 21 ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In antigen trafficking from the skin , it has been postulated that Langerhans cells / dendritic cells are activated after capturing exogenous antigens , up regulate the expression of the chemokine receptor , CCR 7 , and migrate into lymphoid organs in response to the signaling of a chemokine , CCL 21 , which is expressed in lymphatic vessels and T cell zone stromal cells . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We also report that DC migration could be increased up to 10 fold by preinjection of inflammatory cytokines that increased the expression of the CCR 7 ligand CCL 21 in lymphatic endothelial cells . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We then performed a cell migration assay , F actin polymerization , and a phagokinetic assay on esophageal SCC cell lines in the presence of CCL 21 , a ligand of CCR 7 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NKT cell subsets migrated in response to ligands for the inflammatory chemokine receptors CXCR 3 ( monokine induced by IFN gamma / CXC ligand ( CXCL ) 9 ) and CXCR 6 ( CXCL 16 ) , and regulatory chemokine receptors CCR 7 ( secondary lymphoid tissue chemokine ( SLC ) / CC ligand ( CCL ) 21 ) , CXCR 4 ( stromal cell derived factor 1 / CXCL12 ) , and CXCR 5 ( B cell attracting chemokine 1 / CXCL13 ) ; but not to ligands for other chemokine receptors . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Homeostatic chemokines , such as CXCL 13 , CCL 21 , and CCL 19 , as well as their corresponding receptors , CXCR 5 and CCR 7 , have now been shown to closely cooperate in the development of lymphoid organs and the maintenance of lymphoid tissue microarchitecture . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
BACKGROUND AND AIMS : The chemokines CCL 19 and CCL 21 bind CCR 7 , which is involved in the organization of secondary lymphoid tissue and is expressed during chronic tissue inflammation . ^^^ We investigated the expression of CCL 21 and CCR 7 in chronic hepatitis C . ^^^ RESULTS : In patients with chronic hepatitis C , expression of CCL 21 and CCR 7 was up regulated . ^^^ CONCLUSIONS : Expression of CCL 21 during chronic hepatitis C is implicated in the recruitment of T lymphocytes and the organization of inflammatory lymphoid tissue and may promote fibrogenesis in the inflamed areas via activation of CCR 7 on HSCs . . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Activated DCs home to lymphoid organs , where the CCR 7 ligands CCL 19 and CCL 21 are expressed . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CC chemokine ligand 21 ( CCL 21 ) / secondary lymphoid chemokine ( SLC ) , a ligand for CC chemokine receptor 7 ( CCR 7 ) , has been demonstrated to play a vital role in the homing and localization of immune cells to lymphoid tissues , but its role in nonlymphoid tissues largely remains undefined . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Migration of mature DCs to MLNs was specifically blocked by desensitization of CCR 7 with CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In absence of CCR 7 expression , CCL 21 induced chemotaxis of human microglia with efficiency similar to the CXCR 3 ligands CXC chemokine ligand 9 ( monokine induced by IFN gamma ) and CXC chemokine ligand 10 ( IFN gamma inducible protein 10 ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 and its ligands CCL 19 and CCL 21 have been identified as the gatekeepers for both nave T lymphocytes and dendritic cells ( DC ) to these defined anatomical compartments . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 , the receptor for the medullary chemokines CCL 19 and CCL 21 , is induced on thymocytes during their positive selection . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We also detected chemokine receptor CCR 7 on peripheral blood B cells and one of the CCR 7 ligands , CCL 21 , on appendix HEV but not in appendix follicles . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In this study we investigated the early signaling events of CCR 7 initiated by its two endogenous ligands , CCL 19 and CCL 21 . ^^^ However , only activation by CCL 19 , not CCL 21 , promotes robust desensitization of endogenous CCR 7 in the human T cell lymphoma cell line H 9 . ^^^ Taken together , these findings demonstrate that CCL 19 and CCL 21 place CCR 7 in functionally distinct conformations that are independent of their G protein coupling potency : one that allows the efficient desensitization of the receptor and activation of ERK1 / 2 , and another that is impaired in these functions . . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCL 21 chemokine regulates chemokine receptor CCR 7 bearing malignant melanoma cells . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Furthermore , MS loaded mature MoDC expressed normal levels of the chemokine receptor CCR 7 and migrated as efficiently towards CCL 19 or CCL 21 as unloaded MoDC . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Recent studies have implicated the ligands CCL 19 and CCL 21 and their receptor , CCR 7 , in the specific migration of nave lymphocytes and mature dendritic cells to secondary lymphoid organs during immune homeostasis . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Antibodies against ICAM 1 , MAdCAM 1 , or anti L selectin were administered prior to lymphocyte administration , and in some experiments CCR 7 on T lymphocytes was desensitized by excess CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Following lipopolysaccharide ( LPS ) stimulation , the dendritic cells down regulated CCR 1 , 2 , and 5 expression , up regulated or sustained signals for CCR 7 , and migrated to the constitutively expressed ligands CCL 19 ( MIP 3beta ) and CCL 21 ( secondary lymphoid tissue chemokine ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 expression induced by HIV 1 was sufficient to initiate migration of DCs in the presence of secondary lymphoid tissue chemokine ( CCL 21 ) and MIP 3beta ( CCL 19 ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Then genomic sequences of hSLC and mSLC , and of human CCR 7 ( hCCR 7 ) and murine CCR 7 ( mCCR 7 ) , the receptor for SLC , were aligned . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
BCR / ABL mediated downregulation of genes implicated in cell adhesion and motility leads to impaired migration toward CCR 7 ligands CCL 19 and CCL 21 in primary BCR / ABL positive cells . ^^^ Compared with GFP UCB or normal ( NL ) BM CD34+ cells , p 210 UCB and CML CD34+ cells migrated poorly towards the CCR 7 ligands , CCL 19 and CCL 21 , suggesting a possible role for CCR 7 in the abnormal migratory behavior of CML CD34+ cells . . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In contrast , PC lacked CXCR 5 and CCR 7 , and consequently exhibited minimal migration towards CXCL 13 and CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 , CXCR 3 , CCR 5 and CCL 21 expression in synovial tissue from six patients with JIA was investigated by immunohistochemistry . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
At 24 h , IFNalpha had increased B cell chemotaxis to CCL 20 by 20 + / 6 . 2 % ( n = 9 , P < 0 . 002 ) , to CCL 21 by 20 + / 8 . 5 % ( n = 14 , P < 0 . 0001 ) and to CXCL 12 by 16 . 3 + / 4 . 2 % ( n = 12 , P < 0 . 003 ) without changing CCR 6 , CCR 7 or CXCR 4 expression . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Expression of the chemokine receptors CCR 2 and CCR 5 by DC and their responsiveness to the respective ligands , CCL 2 and CCL 3 , were downregulated , while the level of CCR 7 and the DC response to its ligand CCL 21 were enhanced . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
This contact is the result of highly coordinated migration processes , in which the chemokine receptor CCR 7 and its ligands , CCL 19 and CCL 21 , play a central role . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The CCR 7 ligand designated CCL 21 is considered the most important trigger because it appears constitutively expressed by murine HEVs . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In comparison with DCs matured with TNF alpha , IL 1beta , IL 6 and PGE 2 , our DCs showed a weaker in vitro migratory response to CCL 21 , despite comparable CCR 7 expression , and higher allostimulatory and TH 1 polarisation capacities . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Furthermore , the calcium mobilization and chemotactic responses of LPS stimulated DCs to secondary lymphoid tissue chemokine ( SLC ) / CC chemokine ligand 21 ( CCL 21 ) were significantly lower in TPT treated than untreated DCs , in association with lower chemokine receptor 7 ( CCR 7 ) and higher CCR 5 expression . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The CCR 7 ligands , CC chemokine ligand ( CCL ) 19 and CCL 21 , facilitate migration of mature DC from the peripheral tissues to regional lymph nodes . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Comparative analysis also revealed that FastDC possess an equal capacity to migrate in response to the chemokine receptor CCR 7 ligand 6Ckine . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CCR 7 ligands , CCL 19 and CCL 21 , induce not only chemotaxis but also endocytosis in mature DCs . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Interestingly , solar simulated UV radiation also caused an altered migratory phenotype , with an increased expression of CXCR 4 , and a lack of induction of CCR 7 , thus correlating with a high chemotactic response to stromal cell derived factor 1 ( SDF 1 ) ( CXCL 12 ) , but not to secondary lymphoid tissue chemokine ( SLC ) ( CCL 21 ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Recent studies have suggested that a chemokine ligand for the CCR 7 ( CCL 21 ) present on T cells and dendritic cells is important in activating and regulating immunity . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Furthermore , expression of CCL 19 , CCL 20 , and CCL 21 as well as their receptors CCR 6 ( for CCL 20 ) and CCR 7 ( for CCL 19 and CCL 21 ) was characterised by immunohistochemistry and , in addition , their cellular localisation was determined by double immunofluorescence investigations . ^^^ Increased expression of the CCR 7 ligands CCL 19 by DC themselves as well as CCL 21 by reticular cells and lymphatic vessels was observed in Crohn ' s disease , thereby causing the matured DC to be trapped at the site of inflammation . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CD 3 , CD 83 , HECA 452 , VEGFR 3 , SLC , ELC , and CCR 7 expression was studied immunohistochemically . ^^^ SLC , ELC , and CCR 7 mRNA was significantly higher in CD MLNs compared with UC . ^^^ CONCLUSION : The dominant Th 1 immune response is facilitated by interaction of SLC positive HEVs / lymphatic vessels , ELC positive mature DCs , and CCR 7 positive T cells in hyperplastic T cell areas . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We engrafted fully allogeneic islets into streptozotocin treated normal mice or mice with the autosomal recessive paucity of lymph node T cell ( plt ) mutation ; the latter lack secondary lymphoid expression of the CCR 7 ligands , secondary lymphoid organ chemokine ( CCL 21 ) and EBV induced molecule 1 ligand chemokine ( CCL 19 ) . plt mice showed permanent survival of islets engrafted under the kidney capsule , whereas controls rejected islet allografts in 12 days ( p < 0 . 001 ) , and consistent with this , plt mice had normal allogeneic T cell responses , but deficient migration of donor dendritic cell to draining lymph nodes . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
This study shows that ZAP 70 ( + ) CLL B cells respond in vitro more readily than ZAP 70 ( ) CLL and normal B cells to chemokine migratory signals through enhanced surface CCR 7 expression ( P = . 009 ; P < . 001 ) and increased responsiveness to its ligands CCL 19 and CCL 21 , demonstrated by F actin polymerization ( P < . 05 ) and cellular migration ( P < . 01 ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
They strongly express CD 83 , CD 86 , and CCR 7 and have potent ability to migrate to CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In this study , we demonstrate that the migration of human monocyte derived ( Mo ) DCs as well as of ex vivo peripheral blood DCs toward CCL 21 , CXCL 12 , and C5a is stringently dependent on the presence of the proinflammatory mediator PGE 2 , although DCs expressed CXCR 4 and C5aR on their surface and DC maturation was accompanied by CCR 7 up regulation independently of PGE 2 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
METHODS : The expression of two chemokine receptors , CCR 7 and CXCR 4 , and their respective ligands , CCL 19 , CCL 21 , and CXCL 12 , were examined in surgical lung biopsies ( SLBs ) from patients with IIP . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Migration of DC to lymph nodes is regulated by chemokine receptor CCR 7 , expressed on mature DC , and the CCR 7 ligands CCL 19 and CCL 21 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Remarkably , when CCR 7 function was abrogated in vivo by treatment of WT recipients with antibodies to CCR 7 ligands CCL 19 and CCL 21 , lesion size and foam cell content were substantially preserved . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Homing of lymphocytes from blood into secondary lymphoid tissues beyond high endothelial venules is highly dependent on the interaction between the chemokines CCL 19 , CCL 21 , CXCL 12 , and CXCL 13 , and their receptors CCR 7 , CXCR 4 and CXCR 5 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Induction of CCL 21 ( SLC ) and CXCR 12 ( SDF1alpha ) in injured lung tissue , and their respective cognate receptors , CCR 7 and CXCR 4 , in fibroblasts from injured lungs , suggests recruitment of these extrapulmonary progenitor cells via these chemokines . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The expression of CCR 7 by these cells may be induced by the hepatic microenvironment to facilitate recirculation . 3 ) The CCR 7 ligands CCL 19 and CCL 21 are present on lymphatic , vascular , and sinusoidal endothelium in normal liver and in patients with HCV infection . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The chemokines CCL 19 , CCL 21 and CCL 25 , by signalling through the receptors CCR 7 or CCR 9 , play critical roles in leukocyte homing . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Unexpectedly , CCL 21 , a CCR 7 ligand , was expressed not by Foxn 1 dependent thymic primordium but by Gcm 2 dependent parathyroid primordium , whereas CCL 25 , a CCR 9 ligand , was predominantly expressed by Foxn 1 dependent thymic primordium , revealing the role of the adjacent parathyroid in guiding fetal thymus colonization . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
In vitro , LP DCs expressing CCR 7 migrated toward CCL 21 , although the LP DCs appeared morphologically and phenotypically immature . ^^^ In MLNs , DCs bearing the unique LP DC phenotype ( CD11chighCD8alphaintCD11blowalphaLlowbeta7high and CD11chighCD8alpha CD11bhighalphaLlowbeta7high ) were abundant in wild type mice , but were markedly fewer in CCL 19 , CCL 21 Ser deficient plt / plt mice and were almost absent in CCR 7 deficient mice , indicating the critical importance of CCR 7 in LP DC trafficking to MLNs . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
The chemokine receptor CCR 7 and its ligands CCL 19 and CCL 21 play a crucial role for the homing of lymphocytes and dendritic cells to secondary lymphoid tissues . ^^^ In this study , we demonstrate that CCR 7 ( GFP ) is endocytosed into early endosomes containing transferrin receptor upon CCL 19 binding , but less upon CCL 21 triggering . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
CC chemokine receptor 7 ( CCR 7 ) , which regulates the trafficking of leucocytes to the secondary lymphoid organs , has two endogenous chemokine ligands : CCL 19 and CCL 21 . ^^^ Here , we show that substituting N terminal six amino acids of CCL 21 ( SDGGAQ ) for the corresponding N terminal domain of CCL 19 ( GTNDAE ) results in a chimeric chemokine that exhibits high affinity binding and G protein activation of CCR 7 . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
At the same time , the CXCR 5 ( hi ) cells showed reduced responsiveness to the T zone chemokines , Epstein Barr virus induced molecule 1 ( EBI 1 ) ligand chemokine ( ELC ) and secondary lymphoid tissue chemokine ( SLC ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
We now find that the plt defect is due to the loss of both SLC and EBI 1 ligand chemokine ( ELC ) expression in secondary lymphoid organs . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
Surprisingly , we found that for all maturation stimuli tested , the addition of prostaglandin E 2 ( PGE 2 ) was required for effective migration of MoDCs toward the lymph node derived chemokines CCL 19 ( EBI 1 ligand chemokine / macrophage inflammatory protein 3beta ) and CCL 21 ( secondary lymphoid tissue chemokine [ SLC ] / 6Ckine ) . ^^^
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
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Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
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Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
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Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA
Interacting proteins: P32248 and O00585	Pubmed	SVM Score :0.0
NA