| Interacting proteins: P32121 and P53779 |
Pubmed |
SVM Score :0.0 |
| Beta arrestin 2 : a receptor regulated MAPK scaffold for the activation of JNK 3 . beta Arrestins , originally discovered in the context of heterotrimeric guanine nucleotide binding protein coupled receptor ( GPCR ) desensitization , also function in internalization and signaling of these receptors . ^^^ We identified c Jun amino terminal kinase 3 ( JNK 3 ) as a binding partner of beta arrestin 2 using a yeast two hybrid screen and by coimmunoprecipitation from mouse brain extracts or cotransfected COS 7 cells . ^^^ Cellular transfection of beta arrestin 2 caused cytosolic retention of JNK 3 and enhanced JNK 3 phosphorylation stimulated by ASK 1 . ^^^ Moreover , stimulation of the angiotensin 2 type 1A receptor activated JNK 3 and triggered the colocalization of beta arrestin 2 and active JNK 3 to intracellular vesicles . ^^^ |
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| Interacting proteins: P32121 and P53779 |
Pubmed |
SVM Score :0.0 |
| Recently , we identified the c Jun N terminal kinase 3 ( JNK 3 ) as a beta arrestin 2 interacting protein in yeast two hybrid and co immunoprecipitation studies . ^^^ Beta arrestin 2 acts as a scaffold to enhance signaling to JNK 3 stimulated by overexpression of the MAP 3 kinase ASK 1 or by agonist activation of the angiotensin 1A receptor . ^^^ Whereas beta arrestin 2 is a very strong activator of JNK 3 signaling , beta arrestin 1 is very weak in this regard . ^^^ The data also indicate that the specific step enhanced by beta arrestin 2 involves phosphorylation of JNK 3 by the MAP 2 kinase MKK 4 . ^^^ We reasoned that defining the region ( or domain ) in beta arrestin 2 responsible for high level JNK 3 activation would provide insight into the mechanism by which beta arrestin 2 enhances the activity of this signaling pathway . ^^^ |
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| Interacting proteins: P32121 and P53779 |
Pubmed |
SVM Score :0.0 |
| Characterization of a leucine rich nuclear export signal in beta arrestin 2 . beta arrestins ( betaarrs ) are two highly homologous proteins that uncouple G protein coupled receptors from their cognate G proteins , serve as adaptor molecules linking G protein coupled receptors to clathrin coat components ( AP 2 complex and clathrin ) , and act as scaffolding proteins for ERK1 / 2 and JNK 3 cascades . ^^^ |
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| Interacting proteins: P32121 and P53779 |
Pubmed |
SVM Score :0.0 |
| In addition , our data showed that nucleocytoplasmic shuttling of beta arrestin 2 was required , via protein / protein interaction , for the cytoplasmic relocalization of Mdm 2 and JNK 3 , another well known beta arrestin 2 binding protein . ^^^ Our study thus suggests that both the nuclear export signal motif and the N domain of beta arrestins are critical for the regulation of their subcellular localization and that beta arrestin 2 may modulate the function of its binding partners such as Mdm 2 and JNK 3 by alteration of their subcellular distribution . . ^^^ |
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| Interacting proteins: P32121 and P53779 |
Pubmed |
SVM Score :0.0 |
| Dynamic interaction between the dual specificity phosphatase MKP 7 and the JNK 3 scaffold protein beta arrestin 2 . ^^^ The G protein coupled receptor ( GPCR ) adaptor beta arrestin 2 is also a JNK 3 scaffold . ^^^ MKP 7 dephosphorylates JNK 3 bound to beta arrestin 2 , either following activation by ASK 1 overexpression or following AT1aR stimulation . ^^^ |
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| Interacting proteins: P32121 and P53779 |
Pubmed |
SVM Score :0.0 |
| In addition , two known JNK 3 interacting proteins , beta arrestin 2 and JIP 3 , play established roles in neurite outgrowth and neurological development . ^^^ |
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| Interacting proteins: P32121 and P53779 |
Pubmed |
SVM Score :0.0 |
| The AT1R translocates into clathrin coated pits and internalizes upon recruitment of beta arrestin 2 which then recruits ASK 1 and JNK 3 . ^^^ |
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