| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| We now determine that CRP also interacts with FcgammaRIIa ( CD 32 ) , the low affinity receptor for IgG on monocytes and neutrophils . ^^^ CRP binding and the presence of CD 32 were detected by mAb and analyzed by two color flow cytometry . ^^^ Cells expressing CD 32 bound CRP in a dose dependent and saturable manner consistent with receptor binding . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| Uptake of the CRP / LDL coincubate was mediated by the CRP receptor CD 32 . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescence microscopy and flow cytometry analysis established that the uptake of the LDL CRP complex was not mediated by the CRP receptor CD 32 . ^^^ These observations with immobilized CRP and LDL , approximating the conditions that exist in the extracellular matrix of the arterial wall , thus suggest that CRP may contribute to the formation of foam cells in atherosclerotic lesions by causing the aggregation of LDL molecules that are then taken up by macrophages through a CD 32 independent pathway . . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| Cells were exposed to CRP from 0 to 250 micro g / ml for 0 60 min at 37 degrees C and analysed for ( a ) CD11b , PECAM 1 ( CD 31 ) and CD 32 expression by flow cytometry and ( b ) adhesion to LPS ( 1 micro g / ml ; 0 24 h ) treated human umbilical vein endothelial cells ( HUVEC ) . ^^^ There was no significant difference in CD 32 expression , whereas CD 31 expression decreased after exposure to CRP . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| An anti FcgammaRIII ( CD 16 ) but not an anti FcgammaRII ( CD 32 ) antibody produced a 14 % to 32 % reduction of the mCRP effects ( P < 0 . 05 ) . mCRP but not CRP evoked phosphorylation of p 38 mitogen activated protein kinase , and inhibition of this kinase with SB 203580 reversed the effects of mCRP . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| The major CRP ( C reactive protein ) receptor on leucocytes has been identified as the low affinity IgG receptor Fcgamma receptor 2 ( CD 32 ) . ^^^ Confocal microscopy experiments showed a weak expression of the CD 32 receptor in control BAEC that was slightly increased by 10 microg / ml CRP . ^^^ Addition of CRP to TNF alpha incubated BAEC enhanced the fluorescence signal of the CD 32 receptors . ^^^ The protective effect of 10 microg / ml CRP on eNOS expression in TNF alpha incubated BAEC was prevented by an antibody against CD 32 receptors . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : It has been reported that C reactive protein ( CRP ) binds both leukocyte FcgammaRIIA ( CD 32 ) and the plasma membrane of apoptotic cells . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| Anti CD 32 antibody and curcumin were used to block the potential effects of CRP . ^^^ Anti CD 32 antibody partially blocked CRP induced downregulation of TM and EPCR in HCAECs . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| The mRNA level of CD 32 , the major binding protein for CRP in endothelial cells , increased significantly as measured by Northern blot and Western blot . ^^^ The IkappaB degradation , NF kappaB nuclear translocation and VCAM 1 up regulation induced by CRP were all inhibited by treatment with siRNA against CD 32 . ^^^ CRP also increased the expression of CD 32 , which might serve as the receptor for CRP in endothelial cells and mediated the effects of CRP . . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| Preincubation with antibodies to CD 32 and CD 64 or transient transfection with small interference RNA to CD 32 attenuated CRP induced NF kappaB activity , ICAM , VCAM , and monocyte endothelial cell adhesion under static conditions . ^^^ Furthermore , CRP activated endothelial cells supported monocyte rolling , arrest , and transmigration in shear flow ( 2 dyn / cm2 ) , and this was also inhibited by preincubation with antibodies to CD 32 and CD 64 . ^^^ Thus , in HAEC , CRP upregulates monocyte endothelial adhesion by activation of NF kappaB through engaging the Fcgamma receptors CD 32 and CD64 . . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| CRP ( 100 microg / mL ) significantly upregulated surface expression of Fcgamma receptors , CD 32 , as well as CD 64 on HAECs ( P < 0 . 01 ) . ^^^ Also , preincubation with anti CD 32 and CD 64 antibodies significantly inhibited maximal binding of CRP to HAECs 64 % and 30 % , respectively , whereas antibodies to CD 16 had no effect . ^^^ Also , binding and internalization of biotinylated CRP was confirmed by confocal microscopy and CRP colocalized with CD 32 and CD 64 . ^^^ Most importantly , the increase in interleukin 8 , intercellular adhesion molecule 1 , and vascular cell adhesion molecule 1 and the decrease in eNOS and prostacyclin induced by CRP was abrogated with antibodies to CD 32 and CD 64 . ^^^ CONCLUSIONS : We demonstrate that CRP mediates its biological effects on HAECs via binding and internalization through Fcgamma receptors , CD 32 and CD64 . . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| The goal of this study was to elucidate amino acid residues important for the interaction of CRP with human FcgammaRI ( CD 64 ) and FcgammaRIIa ( CD 32 ) . ^^^ |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P31995 and P02741 |
Pubmed |
SVM Score :0.0 |
| NA |
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