| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| The cDNAs of five subunits ( hRPB 220 , hRPB 140 , hRPB 33 , hRPB 25 , and hRPB14 . 5 ) have been isolated . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Mutations in the three largest subunits of yeast RNA polymerase 2 ( RPB 1 , RPB 2 , and RPB 3 ) were investigated for their effects on RNA polymerase 2 structure and assembly . ^^^ The time course of assembly of RPB 1 , RPB 2 , and RPB 3 was investigated in wild type cells and subsequently in mutant cells . ^^^ The RPB 2 plus RPB 3 subcomplexes accumulated in all RPB 1 assembly mutants at the nonpermissive temperature but not in an RPB 2 or RPB 3 assembly mutant . ^^^ These data indicate that RPB 2 and RPB 3 form a complex that subsequently interacts with RPB 1 during the assembly of RNA polymerase II . . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| A sensitive phenotypic assay has been used to identify mutations affecting transcription initiation in the genes encoding the two large subunits of Saccharomyces cerevisiae RNA polymerase 2 ( RPB 1 and RPB 2 ) . ^^^ The rpb 1 and rpb 2 mutations alter the ratio of transcripts initiated at two adjacent start sites of a delta insertion promoter . ^^^ Of a large number of rpb 1 and rpb 2 mutations screened , only a few affect transcription initiation patterns at delta insertion promoters , and these mutations are in close proximity to each other within both RPB 1 and RPB 2 . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| The two large subunits of RNA polymerase 2 , RPB 1 and RPB 2 , contain regions of extensive homology to the two large subunits of Escherichia coli RNA polymerase . ^^^ We investigated whether suppressor genetics could provide evidence for interactions between specific segments of RPB 1 and RPB 2 in Saccharomyces cerevisiae . ^^^ A plasmid shuffle method was used to screen thoroughly for mutations in RPB 2 that suppress a temperature sensitive mutation , rpb 1 1 , which is located in region H of RPB 1 . ^^^ All six RPB 2 mutations that suppress rpb 1 1 were clustered in region 1 of RPB 2 . ^^^ The location of these mutations and the observation that they were allele specific for suppression of rpb 1 1 suggests an interaction between region H of RPB 1 and region 1 of RPB 2 . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation from 32P labeled cell extracts revealed that three of the subunits , RPB 1 , RPB 2 , and RPB 6 , are phosphorylated in vivo . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Sequence analysis of 10 conditional RPB 1 mutations and 10 conditional RPB 2 mutations revealed that the amino acid residues altered by these distinct mutations are nearly always invariant among eucaryotic RPB 1 and RPB 2 homologs . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Two of these suppressors , SIT 1 and SIT 2 , are encoded by RPB 1 and RPB 2 , the genes for the two largest subunits of RNA polymerase 2 . ^^^ All strains containing suppressor mutations in RPB 1 and RPB 2 have reduced transcription of the INO 1 gene and an inositol requirement . ^^^ The SIT 4 protein is over 50 % identical to the catalytic subunit of bovine type 2A protein phosphatase . sit 4 mutations in combination with suppressor mutations in RPB 1 or RPB 2 ( sit 1 , sit 4 or sit 2 , sit 4 ) are lethal , suggesting an interaction between SIT 4 and RNA polymerase II . . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Although multiple functions have been assigned to the three largest subunits , RPB 1 , RPB 2 , and RPB 3 , the functions of the remaining smaller subunits are unknown . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Far Western blot analysis of S . pombe RNA polymerase 2 using 32P labelled recombinant Rpb 5 fused to glutathione S transferase ( GST ) as a probe , indicated that Rpb 5 binds strongly to membrane immobilized Rpb 1 , Rpb 2 and Rpb 3 and weakly to Rpb 5 and a 15 kDa subunit ( Rpb 8 or Rpb 11 ) . ^^^ In agreement with this result , the 32P labelled Rpb 3 probe showed a strong binding signal against Rpb 5 in addition to Rpb 1 and Rpb 2 . ^^^ These two subunits also bind to the two large subunits , Rpb 1 and Rpb 2 , independently . . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Previously , we cloned and sequenced both cDNA and the genes encoding the four large subunits , Rpb 1 , Rpb 2 , Rpb 3 and Rpb 5 . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| After stepwise dissociation of the Rpb 1 and Rpb 3 tagged RNA polymerases fixed on Ni2+ resin by increasing concentrations of urea or guanidium hydrochloride , Rpb 2 Rpb3 Rpb 11 or Rpb 2 Rpb3 Rpb 11 Rpb10 complexes were obtained . ^^^ Both the Rpb 2 Rpb3 Rpb 11 complex and the free Rpb 1 protein showed DNA binding activity , although the affinity was weaker compared with the intact RNA polymerase . . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Previously we indicated that Rpb 3 also plays an essential role in subunit assembly because it interacts with at least four subunits , two large subunits ( Rpb 1 and Rpb 2 ) and two medium sized subunits ( Rpb 3 and Rpb 5 ) ( 1 ) , and it constitutes a core subassembly consisting of Rpb 2 , Rpb 3 , and Rpb 11 ( 2 ) . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| All eight small sized subunits exhibited binding activities to two large subunits , Rpb 1 and Rpb 2 . ^^^ The results altogether indicate that two large subunits Rpb 1 and Rpb 2 provide the platform for assembly of small subunits and also small subunits interact with each other for limited combinations . ^^^ Direct contact of the two large subunits , Rpb 1 and Rpb 2 , was also demonstrated by cross linking . . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Repeated tertiary fold of RNA polymerase 2 and implications for DNA binding . 10 ray diffraction data from two forms of yeast RNA polymerase 2 crystals indicate that the two largest subunits of the polymerase , Rpb 1 and Rpb 2 , may have similar folds , as is suggested by secondary structure predictions . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Mapping of Rpb 3 and Rpb 5 contact sites on two large subunits , Rpb 1 and Rpb 2 , of the RNA polymerase 2 from fission yeast . [ Rpb 1 and Rpb 2 ] Mapping of the contact sites on two large subunits of the fission yeast Schizosaccharomyces pombe RNA polymerase 2 with two small subunits , Rpb 3 and Rpb 5 , was carried out using the two hybrid screening system in the budding yeast Saccharomyces cerevisiae . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| After microsequencing of proteolytic fragments , a complex multidomain organization was indicated for both of the two large subunits , Rpb 1 and Rpb 2 , with the most available sites of proteolysis in junctions between the conserved sequences among RNA polymerase from both prokaryotes and eukaryotes . ^^^ The cross linking studies indicate the following : ( 1 ) the 3 ' termini of growing RNA chains are most extensively cross linked to the second largest subunit Rpb 2 between amino acids 825 and 994 ; ( 2 ) the regions 298 535 of Rpb 2 and 614 917 of Rpb 1 are cross linked to less extents , suggesting that these regions are situated in the vicinity of the catalytic site . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Rpb 1 and Rpb 2 , the two largest subunits and Rpb 8 , a small subunit shared by all three polymerases , failed to support growth in S . cerevisiae . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| A clamp on the DNA nearer the active center , formed by Rpb 1 , Rpb 2 , and Rpb 6 , may be locked in the closed position by RNA , accounting for the great stability of transcribing complexes . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| In cross linking reactions dependent on transcription , RPB 1 , RPB 2 , and RPB 5 were cross linked with N ( 3 ) RdUTP . ^^^ With 5N ( 3 ) dUTP , only RPB 1 and RPB 2 were cross linked . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Results indicate that the levels are low for the three large subunits , Rpb 1 , Rpb 2 and Rpb 3 , which are the homologues of beta ' , beta and alpha subunits , respectively , of prokaryotic RNA polymerase . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| The transcription of one group of genes including rpb 3 , rpb 4 , rpb 5 , rpb 6 , rpb 7 and rpb 10 is mainly initiated at a single site , while that of the other group of genes for rpb 1 , rpb 2 , rpb 8 , rpb 9 , rpb 11 and rpb 12 is initiated at multiple sites . ^^^ Competitive PCR analysis indicated that mRNAs for Rpb 1 , Rpb 3 , Rpb 7 and Rpb 9 were among the group which had a low abundance , while the levels of Rpb 6 and Rpb 10 mRNAs were about fivefold , and that of Rpb 2 mRNA was about 40 fold higher than the Rpb 3 mRNA level . ^^^ The protein to mRNA ratio or the translation efficiency is low for the rpb 1 , rpb 2 , rpb 3 and rpb 11 genes , encoding the homologues of subunits beta ' , beta , alpha and alpha , respectively , of the prokaryotic RNA polymerase core enzyme . . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Thus , the critical part of Rpb 9 is limited to a N terminal half that contacts the lobe of the second largest subunit ( Rpb 2 ) and forms a beta addition motif with the `` jaw ' ' of the largest subunit ( Rpb 1 ) . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : The RNA polymerase 2 of the fission yeast Schizosaccharomyces pombe consists of 12 Rpb subunits , of which four ( Rpb 1 , Rpb 2 , Rpb 3 and Rpb 11 ) form the assembly and catalytic core and five ( Rpb 5 , Rpb 6 , Rpb 8 , Rpb 10 and Rpb 12 ) are shared among RNA polymerases 1 , 2 and 3 . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Improving phylogenetic inference of mushrooms with RPB 1 and RPB 2 nucleotide sequences ( Inocybe ; Agaricales ) . ^^^ Approximately 3000 bp across 84 taxa have been analyzed for variable regions of RPB 1 , RPB 2 , and nLSU rDNA to infer phylogenetic relationships in the large ectomycorrhizal mushroom genus Inocybe ( Agaricales ; Basidiomycota ) . ^^^ This study represents the first effort to combine variable regions of RPB 1 and RPB 2 with nLSU rDNA for low level phylogenetic studies in mushroom forming fungi . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| When bound to RNAP 2 , Mg ( B ) is coordinated by the beta and gamma phosphates of the NTP , Rpb 1 residues D 481 and D 483 and Rpb 2 residue D 837 . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Lower level relationships in the mushroom genus Cortinarius ( Basidiomycota , Agaricales ) : a comparison of RPB 1 , RPB 2 , and ITS phylogenies . ^^^ The combined analyses of ITS and variable regions of RPB 1 and RPB 2 greatly increase the resolution and nodal support for phylogenies of these closely related species belonging to clades that until now have proven very difficult to resolve with the ribosomal markers , nLSU and ITS . ^^^ We present the first study of the utility of variable regions of the genes encoding the two largest subunits of RNA polymerase 2 ( RPB 1 and RPB 2 ) for inferring the phylogeny of mushroom forming fungi in combination with and compared to the widely used ribosomal marker ITS . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Recently , Rpb 1 and Rpb 2 subunits of RNA polymerase 2 ( RNA Pol 2 ) were shown to mediate RNA interference ( RNAi ) directed chromatin modification but did not affect pre siRNA levels . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| Nine subunits homologous to the other eukaryotic RNA Pol 2 , namely RPB 1 , RPB 2 , RPB 3 , RPB 4 , RPB 5 , RPB 6 , RPB 7 , RPB 8 and RPB 11 , were identified in the purified complex . ^^^ |
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| Interacting proteins: P30876 and P24928 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Using the translated amino acid sequences of the RPB 1 and RPB 2 genes , we have inferred a fungal phylogeny that consists largely of well supported monophyletic phyla . ^^^ |
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