Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.51454974
These studies demonstrate that BN analogues can be conjugated with PEG linkers , radiolabeled with 64Cu , and bind to GRPR . 0.51454974^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Of the various types of potent bombesin ( Bn ) / gastrin releasing peptide receptor antagonists that have been discovered , the desMet 14 methyl ester peptides are devoid of residual agonist activity and are among the most potent in terms of in vitro receptor blockade and also in terms of their prolonged inhibition of bombesin stimulated amylase and protein release in the rat . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
PURPOSE : In view of these findings , we investigated the effects of our synthetic bombesin and gastrin releasing peptide receptor antagonist D Tpi 6 , Leu 13 psi ( CH2NH ) Leu 14 bombesin ( 6 14 ) ( RC 3095 ) on the growth of hormone dependent and hormone independent MXT mouse mammary cancers in vivo . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Isolation of a gastrin releasing peptide receptor from normal rat pancreas . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
To determine the pharmacologic and structural properties of the bombesin receptors expressed in human lung carcinoma cells , cDNA clones encoding a human gastrin releasing peptide receptor ( GRP R ) and a pharmacologically distinct neuromedin B preferring bombesin receptor ( NMB R ) were isolated from a human small cell lung carcinoma cell line ( NCI H 345 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Expression of the gastrin releasing peptide receptor confers a growth response to bombesin in immortalized human bronchial epithelial cells . ^^^ We have introduced a human gastrin releasing peptide receptor expression vector into an immortalized human bronchial epithelial cell normally unresponsive to the ligand bombesin . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Within this region lie the genes for calbindin ( CALB 3 ) , gastrin releasing peptide receptor ( GRPR ) , phosphatidyl inositol glycan class A protein ( PIGA ) , glycine receptor alpha 2 ( GLRA 2 ) , phosphorylase kinase alpha ( PHKA 2 ) , XE 59 ( a gene escaping 10 chromosome inactivation ) , and DXS69E ( 71 7A ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Characterization of gastrin releasing peptide receptor expressed in Sf 9 insect cells by baculovirus . ^^^ In the present study we have expressed the murine gastrin releasing peptide receptor ( mGRP R ) in Sf 9 cells using a recombinant baculovirus and characterized it structurally and functionally . mGRP R was detectible 12 h post infection with recombinant baculovirus carrying mGRP R cDNA and became maximal at 60 h post infection ( Bmax = 6 pmol / mg protein ) , which is a 4 60 fold greater density than is found in native tissues . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Mammalian bombesin like peptides ( gastrin releasing peptide [ GRP ] and neuromedin B [ NMB ] ) and their receptors ( GRP R and NMB R ) can stimulate growth of cultured cells , and have been shown to be part of an autocrine growth regulatory network in some human small cell lung carcinoma cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Both groups respond similarly to either gastrin releasing peptide or neuromedin B , but only the response to neuromedin B in oocytes expressing the esophagus mRNA is not blocked by a specific gastrin releasing peptide receptor antagonist , des Met [ D Phe 6 ] Bn ( 6 13 ) ethyl ester . ^^^ RNA blot hybridization studies using a Swiss 3T3 gastrin releasing peptide receptor cDNA probe show no detectable hybridization in esophagus mRNA samples . ^^^ These data suggest that a gastrin releasing peptide receptor is expressed in the esophagus and that it is distinct from that expressed in Swiss 3T3 cells and may represent a third subtype of mammalian bombesin receptor . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Properties of detergent solubilized gastrin releasing peptide receptor were investigated . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor is rapidly phosphorylated by a kinase other than protein kinase C after exposure to agonist . ^^^ In this study we show that the gastrin releasing peptide receptor ( GRP R ) is rapidly phosphorylated in response to agonist exposure . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
G alpha q antisense inhibited responses mediated by neuromedin B receptor ( NMB R ) by 74 % , though not by gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The conserved NPXnY motif present in the gastrin releasing peptide receptor is not a general sequestration sequence . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Chronic desensitization and down regulation of the gastrin releasing peptide receptor are mediated by a protein kinase C dependent mechanism . ^^^ Three mutant gastrin releasing peptide receptors ( GRP R ) were studied : one whose last 37 carboxyl terminal amino acids were eliminated ( construct MGT 346 ) ; one that replaced all of the carboxyl terminal Ser and Thr eliminated in MGT 346 with Ala , Asn , or Gly ( construct JF 1 ) ; and one that selectively replaced the Ser and Thr of the protein kinase C consensus sequence ( PKC CS ) located within the same region with alanine ( construct TS360AA ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Endocytosis of the gastrin releasing peptide receptor ( GRP R ) may regulate cellular responses to GRP . ^^^ We observed endocytosis in transfected epithelial cells by confocal microscopy using cyanine 3 GRP ( cyanine 3 . 18 labeled gastrin releasing peptide ) and GRP R antibodies . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
In human lung membranes , bombesin , gastrin releasing peptide ( GRP ) and GRP preferring bombesin receptor antagonists displaced [ 125I Tyr 4 ] bombesin binding with high affinities ( 36 177 nM ) , whereas neuromedin B possessed a lower affinity of 2878 nM . [ D F5Phe6 , D Ala 11 ] bombesin ( 6 13 ) methyl ester , the most active GRP preferring bombesin antagonist as yet reported , had the highest affinity among all antagonists tested whereas neuromedin B had the lowest affinity . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Internalization of the gastrin releasing peptide receptor is mediated by both phospholipase C dependent and independent processes . ^^^ Recent results for truncated and mutated gastrin releasing peptide ( GRP ) receptors ( GRP R ) , as well as muscarinic cholinergic receptors , suggest that activation of protein kinase C may be needed for full receptor internalization . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
In the present study , we investigated the nature and the importance of glycosylation of two mammalian bombesin receptors , the neuromedin B receptor ( NMB R ) and the gastrin releasing peptide receptor ( GRP R ) , using chemical cross linking and enzymatic deglycosylation . [ 125I ] ( D Tyr 0 ) NMB cross linked to native NMB R on rat C 6 glioblastoma cells or rat NMB R transfected into BALB 3T3 cells revealed a single broad band , M ( r ) = 63 , 000 , on both cell types that was not altered by DTT . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Three receptors have been identified for bombesin and its analogs , namely gastrin releasing peptide receptor ( GRPR ) , neuromedin B receptor ( NMBR ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We have developed a new immunotherapeutic approach to the treatment of small cell carcinoma of the lung ( SCCL ) by targeting the gastrin releasing peptide receptor ( GRP R ) expressed on the surface of these cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Expression of gastrin releasing peptide receptor gene in developing lung . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor induced internalization , down regulation , desensitization , and growth : possible role for cyclic AMP . ^^^ Stimulation of the gastrin releasing peptide receptor ( GRP R ) in Swiss 3T3 cells resembles that of a number of other recently described G protein coupled receptors , insofar as both the phospholipase C and adenylyl cyclase signal transduction pathways are activated . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
RESULTS : The pancreatic secretory response to vagal stimulation was blocked significantly by each of the following : the ganglionic blocker hexamethonium ( 100 % inhibition ) ; the muscarinic , cholinergic blocker atropine ( 85 % inhibition ) ; the specific cholecystokinin A receptor antagonist L 364 , 718 ( 84 % inhibition ) ; a gastrin releasing peptide receptor blocker ( 91 % inhibition ) ; and a vasoactive intestinal polypeptide polyclonal antibody ( 89 % inhibition ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Previously , two mammalian BN like peptide receptor subtypes , gastrin releasing peptide receptor and neuromedin B receptor , have been cloned and characterized . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The two bombesin receptor subtypes , neuromedin B ( NMB R ) and gastrin releasing peptide ( GRP R ) receptors , bind their respective ligands with high affinity . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Serines and threonines in the gastrin releasing peptide receptor carboxyl terminus mediate internalization . ^^^ In this study , components of the gastrin releasing peptide receptor ( GRP R ) regulating internalization were identified . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor has been previously cloned from both humans and mice . ^^^ We have mapped the mouse gastrin releasing peptide receptor ( Grpr ) locus using a polymorphic CAn repeat located in the 5 ' untranslated region of the gene and a Mus spretus / Mus musculus interspecific backcross . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor signaling resulting in growth inhibition . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Three structurally and pharmacologically distinct bombesin receptor subtypes have been isolated and characterized : the gastrin releasing peptide receptor ( GRP R ) , the neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Agonist binding and protein kinase C activation stimulate phosphorylation of the gastrin releasing peptide receptor at distinct sites . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Expression of gastrin releasing peptide receptor in human skin . ^^^ As gastrin releasing peptide belongs to this family , we searched for the presence and distribution of gastrin releasing peptide receptors ( GRPr ) in the skin of healthy human adults by immunohistochemistry , flow cytometry and electron microscopy . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Expression and characterization of gastrin releasing peptide receptor in normal and cancerous pancreas . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Selective reconstitution of gastrin releasing peptide receptor with G alpha q . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Effect of gastrin releasing peptide receptor number on receptor affinity , coupling , degradation , and modulation . ^^^ Three BALB 3T3 cell lines were made that stably expressed the gastrin releasing peptide receptor ( GRP R ) with receptor numbers varying by 280 fold ( GRP R Low , GRP R Med , and GRP R Hi ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor is differentially coupled to adenylate cyclase and phospholipase C in different tissues . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Identification of four amino acids in the gastrin releasing peptide receptor that are required for high affinity agonist binding . ^^^ The bombesin family of G protein coupled receptors includes the gastrin releasing peptide receptor ( GRP R ) , the neuromedin B receptor ( NMB R ) , bombesin receptor subtype 3 ( BRS 3 ) , and bombesin receptor subtype 4 ( bb 4 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Localization of iodine 125 mIP Des Met 14 bombesin ( 7 13 ) NH 2 in ovarian carcinoma induced to express the gastrin releasing peptide receptor by adenoviral vector mediated gene transfer . ^^^ The gastrin releasing peptide receptor ( GRPr ) has a high affinity for the 14 amino acid bombesin peptide . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The translocation breakpoint on the 10 chromosome occurred in the first intron of the gastrin releasing peptide receptor ( GRPR ) gene and that on chromosome 8 occurred approximately 30 kb distal to the 3 ' end of the Syndecan 2 gene ( SDC 2 ) , also known as human heparan sulfate proteoglycan or fibroglycan . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Expression of mRNA for gastrin releasing peptide receptor by human bronchial epithelial cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
To elucidate the molecular basis underlying this selectivity , different Gq / 11 coupled receptors ( m 3 muscarinic , V1a vasopressin , and gastrin releasing peptide receptor ) were coexpressed ( in COS 7 cells ) with mutant alphas subunits in which residues present at the C terminus of alphas were replaced with the corresponding alphaq / 11 residues . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Generation and characterization of mice lacking gastrin releasing peptide receptor . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Constitutive activation of the gastrin releasing peptide receptor expressed by the nonmalignant human colon epithelial cell line NCM 460 . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Bombesin like peptide receptors cloned so far include , gastrin releasing peptide receptor ( GRP R ) , neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Construction of chimeric human bombesin receptors to identify neuromedin B and gastrin releasing peptide receptor binding sites . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
BACKGROUND : Gastrin releasing peptide receptors ( GRPR ) are overexpressed in small cell lung carcinoma and some other human cancers . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Tumor localization of a radiolabeled bombesin analogue in mice bearing human ovarian tumors induced to express the gastrin releasing peptide receptor by an adenoviral vector . ^^^ BACKGROUND : The adenoviral vector , AdCMVGRPr , has been used to induce the expression of the murine gastrin releasing peptide receptor ( GRPr ) both in vitro and in vivo . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Activation of c Jun N terminal kinase is dependent on the expression of the gastrin releasing peptide receptor in rat 1A fibroblasts , demonstrating that the responses to the peptide are receptor mediated . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The subsequent identification of YAC positive clones from this region allowed us to refine the map and localize FIGF centromeric to the phosphatidylinositol glycan complementation class A ( PIGA ) gene and telomeric to the gastrin releasing peptide receptor ( GRPR ) gene . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
In situ hybridization for gastrin releasing peptide receptor ( GRP receptor ) expression in prostatic carcinoma . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Loss of bombesin induced feeding suppression in gastrin releasing peptide receptor deficient mice . ^^^ The gastrin releasing peptide receptor ( GRP R ) is one of three members of the mammalian bombesin subfamily of seven transmembrane G protein coupled receptors that mediate diverse biological responses including secretion , neuromodulation , chemotaxis , and growth . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The mammalian bombesin ( Bn ) like neuropeptide receptors gastrin releasing peptide receptor ( GRP R ) and neuromedin B receptor ( NMB R ) transduce a variety of physiological signals that regulate secretion , growth , muscle contraction , chemotaxis and neuromodulation . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Four subtypes of bombesin receptors are identified ( gastrin releasing peptide receptor , neuromedin B receptor , the orphan receptor bombesin receptor subtype 3 ( BB 3 or BRS 3 ) and bombesin receptor subtype 4 ( BB 4 ) ) , however , only the pharmacology of the gastrin releasing peptide receptor has been well studied . ^^^ In rat pancreatic acini containing only gastrin releasing peptide receptor and in BB 4 transfected BALB cells , this ligand and 125I [ Tyr 4 ] bombesin , the conventional gastrin releasing peptide receptor ligand , gave similar results for receptor number , affinity for bombesin and affinity for the unlabeled ligand . ^^^ The unlabeled ligand was an agonist only at the gastrin releasing peptide receptor in rat acini and did not interact with CCK ( A ) receptors or muscarinic M 3 acetylcholine receptors to increase [ 3H ] inositol phosphates . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Three mammalian bombesin receptor subtypes have been characterized : the gastrin releasing peptide receptor ( GRP R ) , the neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^ In a previous report we identified four amino acids that are critical for high affinity binding of bombesin and gastrin releasing peptide ( GRP ) to the GRP R . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The effects of a potent specific gastrin releasing peptide receptor antagonist , BIM 26226 ( [ D F 5 Phe6 , D Ala 11 ] bombesin ( 6 13 ) OMe ) , and the long acting somatostatin analogue , lanreotide ( BIM 23014 ) , on the growth of an acinar pancreatic adenocarcinoma growing in the rat or cultured in vitro were investigated . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Exclusion of the gastrin releasing peptide receptor ( GRPR ) locus as a candidate gene for Rett syndrome . ^^^ The gene for the gastrin releasing peptide receptor ( GRPR ) has been mapped to a candidate region for Rett syndrome ( RTT ) on the short arm of the 10 chromosome . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Similar experiments with the expressed gastrin releasing peptide receptor and muscarinic m 1 receptor revealed the coupling of Galphaq and Galpha 11 , but not Galpha 14 , to these receptors in oocytes . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The purpose of our present study was to determine : ( 1 ) whether the gastrin gene and peptide could be detected in ovarian cancer cell lines , ( 2 ) if functional gastrin releasing peptide receptors ( GRP R ) are present , and ( 3 ) whether gastrin gene expression is altered by GRP . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Role of receptor and protein kinase C activation in the internalization of the gastrin releasing peptide receptor . ^^^ The bombesin ( Bn ) / gastrin releasing peptide receptor GRP R , which is coupled to phospholipase C via the Gq family of transducing proteins , is internalized rapidly after Bn binding . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Adenoviral mediated delivery of gastrin releasing peptide receptor results in specific tumor localization of a bombesin analogue in vivo . ^^^ In this regard , a radiolabeled bombesin analogue was used in conjunction with a recombinant adenoviral vector encoding the murine gastrin releasing peptide receptor ( mGRPr ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Amplification of mRNA from a human antral cell culture preparation demonstrated the presence of two receptors of the bombesin and gastrin releasing peptide family , GRPR 1 and BRS 3 . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Mice administered a control adenoviral vector encoding the gastrin releasing peptide receptor did not have tumor localization of [ ( 111 ) In ] DTPA D Phe 1 octreotide ( < 1 . 6 % ID / g ) , demonstrating specificity of [ ( 111 ) In ] DTPA D Phe 1 octreotide for the AdCMVhSSTr 2 induced tumor cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
This family of heptahelical , G protein coupled receptors includes the gastrin releasing peptide receptor ( GRP R , or bb 2 ) , neuromedin B receptor ( NMB R , or bb 1 ) , and the bombesin receptor subtype 3 ( BRS 3 , or bb 3 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The 14 aminoacid peptide bombesin ( BN ) has a high affinity for the gastrin releasing peptide receptor which is expressed by a variety of tumours . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
To this end , an adenoviral vector ( AdCMVGRPr ) encoding the murine gastrin releasing peptide receptor ( GRPr ) was used to achieve a high level of binding of radiolabeled bombesin ( BBN ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
An aspartate residue at the extracellular boundary of TMII and an arginine residue in TMVII of the gastrin releasing peptide receptor interact to facilitate heterotrimeric G protein coupling . ^^^ The mammalian bombesin receptor subfamily of G protein coupled receptors currently consists of the gastrin releasing peptide receptor ( GRP R ) , neuromedin B receptor , and bombesin receptor subtype 3 . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Epithelial cells lining the GI tract except in the gastric antrum do not normally express gastrin releasing peptide receptors ( GRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Because it had been shown previously that either synthetic analogs of bombesin ( Bn ) or other receptor peptoid or receptor antagonists function as an antagonist or agonist depends on animal species and receptor subtype studied , we investigated the pharmacological properties of PD 168368 compared with all currently known Bn receptor subtypes ( NMB receptor , gastrin releasing peptide receptor , Bn receptor subtype 3 , and Bn receptor subtype 4 ) from human , mouse , rat , and frog . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Bombesin ( BN ) is a 14 amino acid neuropeptide with a high affinity for the gastrin releasing peptide receptor . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Phosphorylation uncouples the gastrin releasing peptide receptor from G ( q ) . ^^^ In this study we demonstrate that for the G ( q ) coupled gastrin releasing peptide receptor ( GRP R ) , phosphorylation by GRK 2 to a stoichiometry of approximately 1 mol PO ( 4 ) / mol GRP R is sufficient in the absence of arrestin to reduce the rate of receptor catalyzed G protein activation by approximately 80 % . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Epithelial cells lining the adult human colon do not normally express gastrin releasing peptide ( GRP ) or its receptor ( GRPR ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide ( GRP ) regulates critical gastrointestinal functions via the GRP receptor ( GRPR ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We used an in situ reconstitution assay to examine the receptor coupling to purified G protein alpha subunits by the bombesin receptor family , including gastrin releasing peptide receptor ( GRP R ) , neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
High gastrin releasing peptide receptor mRNA level is related to tumour dedifferentiation and lymphatic vessel invasion in human colon cancer . ^^^ Through pharmacological testing , 20 40 % of human colorectal tumours have been shown to be equipped with bombesin / gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
METHODS : A total of 26 human pancreatic adenocarcinomas , obtained after resection , and 5 pancreatic cancer xenografts were analyzed for expression of NTR , vasoactive intestinal peptide receptor ( VIPR ) , substance P receptor ( SPR ) , and gastrin releasing peptide receptor ( GRPR ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
In this study , we used the reverse transcription polymerase chain reaction ( RT PCR ) method to evaluate the mRNA expression of three bombesin receptor subtypes : gastrin releasing peptide receptor ( GRPR ) , neuromedin B receptor ( NMBR ) , and bombesin receptor subtype 3 ( BRS 3 ) , in 22 specimens of human epithelial ovarian cancer and in two human ovarian cancer lines . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Male mice lacking the gastrin releasing peptide receptor ( GRP R ) display elevated preference for conspecific odors and increased social investigatory behaviors . ^^^ Previously , we generated gastrin releasing peptide receptor null mutant mice ( GRP R deficient mice ) , and found that these animals displayed increased non aggressive social responses in an ordinary social interaction test using a resident intruder method . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The hormone bombesin ( BBS ) and its mammalian equivalent gastrin releasing peptide ( GRP ) act through specific GRP receptors ( GRP R ) to affect multiple cellular functions in the gastrointestinal tract ; the intracellular signaling pathways leading to these effects are not clearly defined . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Two amino acids in the sixth transmembrane segment of the mouse gastrin releasing peptide receptor are important for receptor activation . ^^^ The gastrin releasing peptide receptor ( GRP R ) is a G protein coupled receptor that mediates a variety of cellular responses , including cell growth and modulation of neuronal activity by activation of heterotrimeric GTP binding proteins in the Gq family . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Sex specific expression of gastrin releasing peptide receptor : relationship to smoking history and risk of lung cancer . ^^^ BACKGROUND : Activation of gastrin releasing peptide receptor ( GRPR ) in human airways has been associated with a proliferative response of bronchial cells to gastrin releasing peptide and with long term tobacco use . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Three bombesin receptor subtypes , termed gastrin releasing peptide receptor ( GRPR ) , neuromedin B receptor ( NMBR ) , and bombesin receptor subtype 3 ( BRS 3 ) , have been identified in rodents and humans . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Molecular organization of the mouse gastrin releasing peptide receptor gene and its promoter . ^^^ The murine gastrin releasing peptide receptor ( mGRP R ) is a member of the G protein coupled receptor family and mediates important physiological actions of its specific ligand , the gastrointestinal hormone / neurotransmitter GRP , including mitogenic properties in the mouse Swiss 3T3 fibroblasts . ^^^ We isolated and characterized genomic bacteriophage P 1 clones encoding the mouse gastrin releasing peptide receptor ( mGRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
PAMP showed affinity for gastrin releasing peptide preferring receptor ( GRP R ) and neuromedin B preferring receptor . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Adenovirus vectors encoding the genes for a variety of antigens / receptors ( carcinoembryonic antigen , gastrin releasing peptide receptor , somatostatin receptor subtype 2 ( SSTr 2 ) ) have all shown that their expression is increased on cancer cells both in vitro and in vivo following adenovirus infection . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
In order to specify these functions , our group and another laboratory generated by gene targeting mutant mice that lack one of the three BN like peptide receptors found in mammals , ie neuromedin B receptor ( NMB R ; BB 1 ) , gastrin releasing peptide receptor ( GRP R ; BB 2 ) , or bombesin receptor subtype 3 ( BRS 3 ; BB 3 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Molecular basis for selectivity of high affinity peptide antagonists for the gastrin releasing peptide receptor . ^^^ The receptor mediating the action of the mammalian bombesin ( Bn ) peptide , gastrin releasing peptide receptor ( GRPR ) , is an exception , because numerous classes of peptide antagonists are described . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
METHODS : ( 1 ) RT PCR and Southern blot hybridization were performed for detection of gastrin releasing peptide GRP R mRNA ; ( 2 ) Covalent cross linking study with biotinyl bombesin and enhanced chemoluminescence ( ECL ) was performed to identify GRP R at protein levels in SGC 7901 cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We assessed the role of BN like peptides / receptors in emotional and / or anxiety related behavior using three strains of knockout mice , each deficient in a single BN like peptide receptor ( gastrin releasing peptide receptor , bombesin receptor subtype 3 , or neuromedin B receptor ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
To investigate the molecular basis for its selectivity for the NMBR over the closely related receptor for gastrin releasing peptide ( GRPR ) , we used a chimeric receptor approach and a site directed mutagenesis approach . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The broad spectrum antagonist of neuropeptide receptor , [ D Arg 1 , D Phe 5 , D Trp 7 , 9 , Leu 11 ] substance P , induced apoptosis selectively in human small cell lung carcinoma ( SCLC ) cells , which express gastrin releasing peptide receptor , but not in other types of tumor cells as well as normal cells . ^^^ Thus , blockage of gastrin releasing peptide receptor mediated signaling may provide a novel therapeutic option in SCLC which has become resistant to conventional chemotherapeutic agents . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We now investigate gene expression and function of BLP ( gastrin releasing peptide , GRP ) and BLP receptors ( GRP R and BRS 3 ) in lung from two baboon BPD models . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Glycosylation of the gastrin releasing peptide receptor and its effect on expression , G protein coupling , and receptor modulatory processes . ^^^ We used site directed mutagenesis to address these issues with the gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor mediated autocrine growth in squamous cell carcinoma of the head and neck . ^^^ BACKGROUND : Gastrin releasing peptide receptor ( GRPR ) mediated autocrine growth appears to be an early marker of susceptibility to tobacco related lung cancers . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Recent studies have shown that aberrantly expressed gastrin releasing peptide ( GRP ) and its receptor ( GRP R ) critically regulate tumor cell differentiation in colon cancers developing in humans and mice . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Inhibition of proliferation of human small cell lung cancer cells expressing an autocrine system for gastrin releasing peptide by antisense oligodeoxynucleotides to gastrin releasing peptide receptor . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Regulation of gastric function by endogenous gastrin releasing peptide in humans : studies with a specific gastrin releasing peptide receptor antagonist . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Molecular basis of the selectivity of gastrin releasing peptide receptor for gastrin releasing peptide . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Disruptions in feeding and body weight control in gastrin releasing peptide receptor deficient mice . ^^^ Bombesin ( BN ) interacts with two mammalian receptor subtypes termed gastrin releasing peptide ( GRP ) preferring ( GRP R ) and neuromedin B ( NMB ) preferring ( NMB R ) that may mediate the satiety action of BN . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The orphan receptor , human bombesin receptor subtype 3 ( BRS 3 ) was assigned to the G protein coupled bombesin receptor family because of its high sequence homology with the neuromedin B receptor ( NMB R ) and gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Human gastrin releasing peptide receptor mediates sustained CREB phosphorylation and transactivation in HuTu 80 duodenal cancer cells . ^^^ The G protein coupled human gastrin releasing peptide receptor ( hGRP R ) is frequently found aberrantly expressed in human cancers of the colon , stomach , and lung , and its ligand specific activation has been implicated in cell proliferation and differentiation . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Islet function phenotype in gastrin releasing peptide receptor gene deficient mice . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Expression of progastrin releasing peptide and gastrin releasing peptide receptor mRNA transcripts in tumor cells of patients with small cell lung cancer . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Bombesin ( BBN ) , a 14 amino acid peptide , is an analogue of human gastrin releasing peptide ( GRP ) that binds to GRP receptors ( GRPr ) with high affinity and specificity . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
METHODS : Expression of prepro gastrin releasing peptide ( preproGRP ) , neuromedin B receptor ( NMB R ) and gastrin releasing peptide receptor ( GRP R ) were examined by reverse transcriptase polymerase chain reaction ( RT PCR ) in peripheral blood and bone marrow aspirate from 40 untreated patients with SCLC . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Bombesin ( BBN ) , a 14 amino acid peptide , is an analogue of human gastrin releasing peptide ( GRP ) that binds to GRP receptors ( GRPr ) with high affinity and specificity . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
BACKGROUND : Bombesin ( BBN ) , a 14 amino acid peptide , is an analogue of human gastrin releasing peptide ( GRP ) that binds to GRP receptors ( GRPr ) with high affinity and specificity . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Increased frequency of gastrin releasing peptide receptor gene mutations during colon adenocarcinoma progression . ^^^ Epithelial cells lining the mature human colon do not normally express receptors for gastrin releasing peptide ( GRPR ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
A recessive deafness mutation in the mouse arose spontaneously and was identified in a colony segregating a null allele of the gastrin releasing peptide receptor ( Grpr ) locus . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We analyzed murine mast cells for BLP receptor gene expression , and identified mRNAs encoding bombesin receptor subtype 3 and neuromedin B receptor ( NMB R ) , but not gastrin releasing peptide receptor . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
NE cells express a variety of peptide hormones , including the bombesin ( BBS ) like peptide , gastrin releasing peptide ( GRP ) , and its cognate receptor , GRP R . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
MicroPET imaging of a gastrin releasing peptide receptor positive tumor in a mouse model of human prostate cancer using a 64Cu labeled bombesin analogue . ^^^ The gastrin releasing peptide receptor ( GRPR ) is overexpressed on a variety of carcinomas and has been the target for detection and treatment of these neoplasms in animals . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide ( GRP ) and its receptor ( GRP R ) are not normally expressed by epithelial cells lining the colon but are aberrantly expressed in cancer , where they act as morphogens and regulate tumor cell differentiation . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We found , in this study , bombesin specifically induced mitogen activated protein ( MAP ) kinase activation as shown by increased extracellular regulated kinase ( ERK ) phosphorylation and epidermal growth factor ( EGF ) receptor transactivation in prostate cancer cells , which express functional gastrin releasing peptide receptor . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Nonpeptide gastrin releasing peptide receptor antagonists inhibit the proliferation of lung cancer cells . ^^^ These results indicate that PD 176252 is a gastrin releasing peptide receptor antagonist , which inhibits the proliferation of lung cancer cells . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
To test the hypothesis that such effects are mediated by known mammalian bombesin receptors [ gastrin releasing peptide ( GRP ) / bombesin preferring receptor ( GRPR ) , neuromedin B ( NMB ) receptor ( NMBR ) , and the orphan bombesin receptor subtype 3 ( BRS 3 ) ] , we analyzed the ontogeny of GRPR , NMBR , and BRS 3 gene expression in mouse lung . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Importance of amino acids of the central portion of the second intracellular loop of the gastrin releasing Peptide receptor for phospholipase C activation , internalization , and chronic down regulation . ^^^ We used site directed mutagenesis to address these issues with the gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We previously reported that a gastrin releasing peptide / gastrin releasing peptide receptor ( GRP / GRPR ) autocrine growth pathway is activated early in HNSCC carcinogenesis . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Agonist and protein kinase C induced phosphorylation have similar functional consequences for gastrin releasing peptide receptor signaling via Gq . ^^^ Gastrin releasing peptide receptor ( GRPr ) is phosphorylated by a kinase other than protein kinase C ( PKC ) after exposure to agonist and is also a substrate for PKC dependent phosphorylation after treatment with 12 O tetradecanoylphorbol 13 acetate ( TPA ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor ( GRPR ) locus in Japanese subjects with autism . ^^^ Gastrin releasing peptide receptor ( GRPR ) gene is considered a candidate locus for infantile autism for several reasons . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
This approach was termed `` Genetic Radioisotope Targeting Strategy . ' ' Using this strategy , induction of high levels of gastrin releasing peptide receptor or human somatostatin receptor subtype 2 expression and selective tumor uptake of radiolabeled peptides was achieved . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Transcriptional activation of the human gastrin releasing peptide receptor gene in gastrointestinal and prostatic epithelial cancer cells . ^^^ The mammalian gastrin releasing peptide receptor ( GRP R ) belongs to the superfamily of G protein coupled receptors and mediates actions of the regulatory GRP and bombesin , the amphibian homolog of GRP . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide and gastrin releasing peptide receptor expression in gastrointestinal carcinoid tumours . ^^^ AIMS : To establish whether gastrin releasing peptide ( GRP ) and the GRP receptor ( GRPR ) are expressed together in gastrointestinal carcinoid tumours . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We have proposed that gastrin releasing peptide ( GRP ) and its receptor ( GRP R ) are morphogens that when aberrantly re expressed in colon cancer promote tumor cell differentiation and retard metastasis . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Overexpression of gastrin releasing peptide ( GRP ) receptor ( GRPR ) in both androgen dependent ( AD ) and androgen independent ( AI ) human neoplastic prostate tissues provides an attractive target for prostate cancer imaging and therapy . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Ets transcriptional regulation of gastrin releasing peptide receptor in neuroblastomas . ^^^ BACKGROUND : We have demonstrated that gastrin releasing peptide ( GRP ) binds specifically to its cell surface receptor , GRP R , to act as an autocrine / paracrine growth factor for neuroblastomas ( NBs ) ; an increased expression of GRP R was found in more advanced stage NBs . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Alterations in receptor expression or agonist concentration change the pathways gastrin releasing peptide receptor uses to regulate extracellular signal regulated kinase . ^^^ In this study , we demonstrate that gastrin releasing peptide receptor ( GRPr ) regulates ERK through multiple pathways in a single cell type depending upon receptor expression and agonist concentration . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Dexamethasone regulation of gastrin releasing peptide receptor in human lung cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor expression is decreased in patients with Crohn ' s disease but not in ulcerative colitis . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We therefore investigated the effect of bombesin ( a homologue of gastrin releasing peptide ) on proliferation , migration and inositol phosphate production in the human colorectal carcinoma cell line DLD 1 , and determined the ability of gastrin releasing peptide receptor antagonists to inhibit these effects . ^^^ We conclude that both amidated and non amidated forms of gastrin releasing peptide accelerate proliferation and migration of DLD 1 human colorectal carcinoma cells via the gastrin releasing peptide receptor , but that phosphatidylinositol 3 kinase is only involved in the cell migration signalling pathway . ^^^ Our results suggest a potential role for gastrin releasing peptide receptor antagonists in the management of colorectal carcinoma . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Peptides showed high affinity for the human GRP R ( GRP R = gastrin releasing peptide receptor ) expressed in PC 3 cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Identification of key amino acids in the gastrin releasing peptide receptor ( GRPR ) responsible for high affinity binding of gastrin releasing peptide ( GRP ) . ^^^ The bombesin ( Bn ) receptor family includes the gastrin releasing peptide ( GRPR ) and neuromedin B ( NMBR ) receptors , Bn receptor subtype 3 ( BRS 3 ) and Bn receptor subtype 4 ( BB ( 4 ) ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Immunohistochemical localization of gastrin releasing peptide receptor in the mouse brain . ^^^ Gastrin releasing peptide ( GRP ) is a mammalian bombesin ( BN ) like peptide that binds with high affinity to the GRP receptor ( GRP R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Bombesin ( BN ) , a 14 amino acid peptide , shows high affinity for the human gastrin releasing peptide receptor ( GRP r ) , which is overexpressed on several types of cancer , including prostate , breast , gastrointestinal , and small cell lung cancer . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The biologic profiles of [ ( 99m ) Tc ] Demobesin 1 ( [ ( 99m ) Tc N ( 4 ) ( 0 1 ) , bzlg ( 0 ) , d Phe ( 6 ) , Leu NHEt ( 13 ) , des Met ( 14 ) ] BB ( 6 14 ) ) and [ ( 111 ) In ] Z 070 were compared using various gastrin releasing peptide receptor GRP R ) expressing tissues of human and animal origin . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Expression of gastrin releasing Peptide receptor in renal cell carcinomas : a potential function for the regulation of neoangiogenesis and microvascular perfusion . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor mediates activation of the epidermal growth factor receptor in lung cancer cells . ^^^ Gastrin releasing peptide receptor ( GRPR ) and the epidermal growth factor receptor ( EGFR ) are expressed in several cancers including non small cell lung cancer ( NSCLC ) . ^^^ Here we demonstrate the activation of EGFR by the GRPR ligand , gastrin releasing peptide ( GRP ) , in NSCLC cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Non associative learning and anxiety in rats treated with a single systemic administration of the gastrin releasing peptide receptor antagonist RC 3095 . ^^^ The gastrin releasing peptide receptor ( GRPR ) has been implicated in the modulation of emotionally motivated memory . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Bombesin is a 14 amino acid peptide with very high affinity for the BB 2 or gastrin releasing peptide receptor ( GRPr ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Overexpression of gastrin releasing peptide receptor induced layer disorganization in brain . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor antagonist effects on an animal model of sepsis . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor ( GRPR ) is found to be overexpressed in a variety of human tumors . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Diurnal regulation of the gastrin releasing peptide receptor in the mouse circadian clock . ^^^ We focus here on gastrin releasing peptide ( GRP ) and on GRP receptor ( GRPr ) expressing cells of the SCN . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor as a molecular target for psychiatric and neurological disorders . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Opposite effects of low and high doses of the gastrin releasing peptide receptor antagonist RC 3095 on memory consolidation in the hippocampus : possible involvement of the GABAergic system . ^^^ Although the gastrin releasing peptide receptor ( GRPR ) has recently emerged as a system importantly involved in regulating memory formation , the role of hippocampal GRPRs in memory remains controversial . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Progastrin releasing peptide and gastrin releasing peptide receptor mRNA expression in non tumor tissues of the human gastrointestinal tract . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Some of them ( namely potassium competitive acid blockers ( P CABs ) and CCK 2 receptor antagonists ) have already reached clinical testing while some others ( like the antigastrin vaccine , H 3 receptor ligands or gastrin releasing peptide receptor antagonists ) are still in preclinical development and need the proof of concept in human beings . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The focus of this study was to evaluate the therapeutic benefit of combined gastrin releasing peptide ( GRP ) receptor targeted radiotherapy ( TRT ) with chemotherapy , using the PC 3 xenograft severe combined immunodeficiency ( SCID ) mouse model . ( 177 ) Lu DOTA 8 AOC BBN ( 7 14 ) NH ( 2 ) is a radiotherapeutic peptide that specifically targets the gastrin releasing peptide receptor overexpressed on primary and metastatic prostate cancer . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Although the gastrin releasing peptide receptor has been implicated in memory consolidation , previous studies have not examined whether it is involved in extinction . ^^^ Here we show that gastrin releasing peptide receptor blockade in the hippocampus disrupts extinction of aversive memory . ^^^ Infusion of a gastrin releasing peptide receptor antagonist or the protein synthesis inhibitor anisomycin into the dorsal hippocampus immediately after the first extinction session blocked extinction . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Consequence of gastrin releasing peptide receptor activation in a human colon cancer cell line : a proteomic approach . ^^^ Gastrin releasing peptide ( GRP ) and its receptor ( GRPR ) are aberrantly up regulated in colon cancer . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptors ( GRP R ) are upregulated in many cancers , including prostate , breast , and lung . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The results of these investigations demonstrate the ability of these new conjugates to specifically target the gastrin releasing peptide receptor subtype 2 , which is overexpressed on human prostate PC 3 cancerous tissues . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Ligand binding studies show that while both receptors can be activated by either GRP or NMB , one receptor has a higher affinity for GRP than for NMB ( GRP R ) , while the other has a higher affinity for NMB than for GRP ( NMB R ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We describe the structure and expression of a cloned NMB preferring bombesin receptor ( NMB R ) with properties distinct from a GRP preferring bombesin receptor ( GRP R ) reported previously . ^^^ The distinct regional distribution of NMB R and GRP R mRNA in the brain suggests that both bombesin receptor subtypes play independent roles in mediating many of the dramatic effects of bombesin like peptides in the central nervous system . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
GRP preferring bombesin receptor subtype mediates contractile activity in cat terminal ileum . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
In total RNA from fresh AP we detected high amounts of NMB mRNA and much smaller amounts of GRP mRNA , while finding a weak signal for GRP receptor ( GRP R ) and NMB receptor ( NMB R ) mRNAs . ^^^ In total RNA from the reaggregate cell cultures we detected high levels of NMB mRNA as well as a strong signal for GRP R mRNA . ^^^ Finally , in GH 3 cells , high levels of NMB mRNA and GRP R mRNA were found , while GRP mRNA and NMB R mRNA remained undetectable even in high amounts ( 200 micrograms ) of total RNA . ^^^ NMB R mRNA and GRP R mRNA are both present in low levels in fresh AP whereas the GRP R mRNA is predominant in GH 3 cells and estradiol treated AP reaggregate cell cultures . ^^^ Compared to fresh AP tissue , NMB mRNA and GRP R mRNA expression is enhanced in estradiol treated reaggregate cell cultures . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We used digoxigenin labeled cRNA probe for GRP and a biotinylated cRNA probe for GRP receptor ( GRP R ) on frozen and / or formalin fixed , paraffin embedded sections of rat brain . ^^^ Antidigoxigenin alkaline phosphatase conjugate and nitro blue tetrazolium , producing a blue color reaction , was used to visualize GRP mRNA , and for GRP R mRNA , avidin alkaline phosphatase and vector red , were used resulting in a vivid red color . ^^^ RESULTS : Strong specific signals for GRP and / or GRP R were detected in rat brain neurons . ^^^ The overall distribution of GRP and GRP R mRNAs in rat brain was similar to that previously reported with single ISH using radioactive labeled probes . ^^^ Double ISH revealed colocalization of the two mRNAs in some neurons with the expression of GRP mRNA often coexpressed with GRP R mRNA . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Recent cloning studies confirm two subtypes of Bn receptors exist , a neuromedin B preferring receptor ( NMB R ) and a gastrin releasing peptide preferring receptor ( GRP R ) . ^^^ Both subtypes occur widely in GI tract and the CNS ; however , in contrast to the GRP R subtype little is known about the ligand receptor interactions for the NMB R . ^^^ Therefore , in the present study we explored the ligand receptor interactions including kinetics , stoichiometry , internalization , degradation and regulation by guanine nucleotide binding proteins with the NMB R and compared it to the GRP R . ^^^ The rat glioblastoma C 6 cell line which possess functional NMB R and 3T3 cells which possess functional GRP R were used . 125I [ D Tyr 0 ] NMB and 125I [ Tyr 4 ] Bn were prepared using Iodogen and purified on HPLC . ^^^ At 37 degrees C binding of 125I [ D Tyr 0 ] NMB to NMB R or 125I [ Tyr 4 ] Bn to GRP R was maximal by 5 15 min and decreased to 60 70 % after 60 min . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The specific GRP R antagonist blocked the response to bombesin in oocytes injected with GRP R , but failed to do so in oocytes injected with NMB R . ^^^ Somatic cell hybrids localized the GRP R on the 10 chromosome and the NMB R on chromosome 6 . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The purpose of our study was to ( 1 ) confirm functional GRP R in SIIA and ( 2 ) determine whether bombesin alters the expression and binding activity of the AP 1 transcription factors , c jun and jun B . ^^^ RESULTS : SIIA cells mobilized intracellular calcium in response to bombesin , exhibiting a functional cell surface GRP R . ^^^ CONCLUSIONS : The SIIA human gastric cancer possesses functional GRP R coupled to the calcium second messenger pathway . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
To determine which of the three known bombesin receptor subtypes ( GRP receptor ( GRP R ) , neuromedin B receptor , bombesin receptor subtype 3 ) were expressed in the cell lines , reverse transcription / PCR analysis of cellular RNA followed by hybridization with receptor specific cDNA was performed . ^^^ This revealed the presence of GRP R transcript in all cell lines , while neither of the other two receptor transcripts were expressed . ^^^ Our data indicate that bombesin is a potent inducer of signal transduction via GRP R receptors in androgen insensitive PC 3 and DU 145 prostate cancer cells . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
For the naturally occurring agonists , at the GRP preferring bombesin receptor the relative affinities were litorin = ranatensin = bombesin > GRP > > neuromedin B , phyllolitorin and at the neuromedin B preferring bombesin receptor were litorin = neuromedin B = ranatensin > bombesin , phyllolitorin > > GRP . ^^^ For the GRP preferring bombesin receptor the heptapeptide and for the neuromedin B preferring bombesin receptor the octapeptide was the minimal carboxyl fragment interacting with the receptor / or causing biologic activity , and the nonapeptide and full decapeptide , respectively , were the minimal required for full affinity . ^^^ A conformationally restricted GRP analogue , [ D Cys 6 , D Ala 11 , Cys 14 ] bombesin ( 6 14 ) had a significantly higher affinity for GRP preferring bombesin receptor than NMB receptor . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Therefore , we examined the uterus and placenta of pregnant women at term for synthesis of GRP and expression of GRP receptor genes BRS 3 and GRP R . ^^^ Minor peaks were also observed : two larger than the main form and one corresponding to GRP ( 18 27 ) . mRNA for GRP receptors GRP R and BRS 3 was detected by semiquantitative reverse transcription PCR . ^^^ GRP R mRNA predominated in the pregnant endometrium , whereas BRS 3 mRNA predominated in the membranes and placenta . ^^^ The detection of mRNA for GRP R , BRS 3 , and possibly a transcript variant of BRS 3 as well as the detection of a peptide larger than , but related to , GRP suggest a novel regulatory unit in the human reproductive tract . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The mechanism is not known , and a potent and specific GRP R antagonist BIM 26226 , which has low affinity for NMB R , was used in vivo in athymic nude mice bearing gastrinoma subcutaneously . ^^^ RT PCR study of gastrinoma revealed the presence of both GRP R and NMB R mRNA , but much more abundant NMB R mRNA . ^^^ We conclude that BBS stimulated growth of gastrinoma involves both GRP R and NMB R , and our findings suggest that GRP R mediates negative and NMB R produces positive growth effects on gastrinoma . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We obtained endoscopic pinch biopsies of , and extracted the RNA from , epithelial cells lining the esophagus , stomach , jejunum , ileum , and proximal and descending colon , RT PCR demonstrated that GRP R expression is limited to cells lining the gastric antrum , indicating that this receptor is aberrantly expressed by GI cancers . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We determined whether a human gastric cancer cell line ( SIIA ) expresses a functional GRP receptor ( GRP R ) . ^^^ BBS increased intracellular calcium ( [ Ca2+ ] 1 ) , and a specific GRP R antagonist , ( [ D Phe 6 , Des Met 14 ] BBS ( 6 14 ) ethylamide ) , blocked BBS induced increase in [ Ca2+ ] 1 . ^^^ SIIA cells possess GRP R mRNA by reverse transcriptase PCR . ^^^ These findings indicate that SIIA cells possess a GRP R that is capable of physiological signal transduction , though the cellular response remains unknown . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
To determine if the expression of bombesin like peptides ( BLP ) and their receptors ( GRP R and NMB R ) correlate with an in vitro response to 2A11 , we measured these parameters in seven SCLC cell lines . ^^^ Calcium mobilization in response to BLP stimulation was detected in the six cell lines expressing either GRP R or NMB R mRNA but not in NCI N 417 , which had no detectable BLP receptor . 2A11 ( 5 microg / ml ) inhibited colony formation by 26 61 % after 2 weeks in all cell lines except NCI N 417 . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Previously , we have generated and characterized mice with targeted disruption of the two other BN like peptide receptors , bombesin receptor subtype 3 ( BRS 3 ) and gastrin releasing peptide preferring receptor ( GRP R ) . ^^^ Here we describe the generation and analysis of NMB R deficient mice to investigate how NMB R differs from BRS 3 and GRP R . ^^^ Compensation for NMB R deficiency by overexpression of GRP R and / or BRS 3 was not detected . ^^^ In addition , the behavioral phenotypes of GRP R deficient mice were not observed in NMB R deficient mice . ^^^ These data show that the functions of NMB R and GRP R are distinct , with only partial overlap . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The pathophysiological relevance of the bombesin / GRP receptor ( GRP R ) , which is expressed in 30 % of human colon tumor cell lines and in 24 40 % of native tumors , has not been clearly assessed at this time . ^^^ Competitive reverse transcription PCR showed a high GRP R mRNA level in Isreco 1 cells , and binding studies confirmed the expression of bombesin / GRP subtype receptors ( Kd = 0 . 42 nM ; Bmax = 18 , 000 sites / cell ) . ^^^ These results clearly indicate that bombesin exerts morphological , adhesive , and proliferative effects on Isreco 1 cells , suggesting that expression of the bombesin / GRP R may contribute to the malignant properties of colon carcinoma cells . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The sublibraries with the highest affinity to the MSH receptor ( i . e . melanocortin type 1 or MC 1 receptor ) and , respectively , the GRP preferring bombesin receptor were identified by an iterative process . ^^^ The KD of the most potent MC 1 receptor ligand was 1 . 58 mumol / l and that of the GRP preferring bombesin receptor 3 . 40 mumol / l . ^^^ These results demonstrate that testing a peptoid library containing 328 , 509 single compounds led to the successful identification of new ligands for both the MC 1 receptor as well as the GRP preferring bombesin receptor . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
We determined the roles of intracellular free Ca ( 2+ ) ( [ Ca ( 2+ ) ] ( 1 ) ) , protein kinase C ( PKC ) , and mitogen activated protein kinases ( MAPK ) in GRP receptor ( GRP R ) regulated secretion . ^^^ GRP R regulated secretion is initiated by an increase in [ Ca ( 2+ ) ] ( 1 ) ; however , elevated [ Ca ( 2+ ) ] ( 1 ) is insufficient to stimulate secretion in the absence of activation of PKC and the downstream MEK / MAPK pathways . ^^^ We demonstrated that the activity of MEK is important for maintaining elevated [ Ca ( 2+ ) ] ( 1 ) levels induced by GRP R activation , suggesting that MEK may affect receptor regulated secretion by modulating the activity of Ca ( 2+ ) sensitive PKC . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
GRP receptor ( GRPR ) mRNA was also expressed in the majority of NSCLC cell lines ( nine of 14 ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Although epithelial cells lining the mouse colon do not normally express GRP and its receptor ( GRP R ) , both are aberrantly expressed by all better differentiated cancers in wild type C57BL / 6J mice treated with the carcinogen azoxymethane . ^^^ Whereas small tumors in both wild type and GRP R deficient ( i . e . , GRP R / ) mice are histologically similar , larger tumors become better differentiated in the former but degenerate into more poorly differentiated mucinous adenocarcinomas in the latter . ^^^ This alteration in phenotype is attributable to GRP increasing focal adhesion kinase expression in GRP R expressing tumors . ^^^ Consistent with GRP acting as a mitogen , GRP / GRP R coexpressing tumors in wild type animals also contain more proliferating cells than those occurring in GRP R / mice . ^^^ Yet tumors are similarly sized in animals of either genotype receiving azoxymethane for identical times , a finding attributable to the significantly higher number of apoptotic cells detected in GRP / GRP R coexpressing cancers . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Although epithelial cells lining the colon do not normally express GRP or its receptor ( GRP R ) , most human tumors express GRP R mRNA . ^^^ To elucidate the reason for the difference between the expression of GRP / GRP R mRNA and protein , we studied nine human colon cancer cell lines . ^^^ Quantitative polymerase chain reaction revealed that all colon cancer cell lines expressed similar amounts of mRNA for both GRP as well as GRP R . ^^^ Conformational fragment length polymorphism analysis indicated that although mRNA for the ligand GRP was never mutated , mRNA for the GRP R was always mutated . ^^^ Sequencing revealed that the message for GRP R contained between two and seven separate mutations at the nucleotide level . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction studies , revealed the presence of mRNA for NMB and for the GRP preferring receptor ( GRP R ) . mRNA for GRP , NMB preferring receptor ( NMB R ) and bombesin receptor subtype 3 ( BRS 3 ) were not detected . [ D Phe ( 6 ) ] bombesin ( 6 13 ) methyl ester ( A 1 ) and BIM 23127 ( A 2 ) , are considered as inhibitors of binding to GRP R and NMB R , respectively . ^^^ Our data demonstrate that the proliferation induced by BLP in HT 29 cells is due to interaction with the GRP R . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Prostatic neuroendocrine cells are thought to secrete these regulatory peptides and they may therefore interact with their specific , aberrantly expressed GRP receptor ( GRP R ) in prostate cancer . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Moreover , we found that GRP receptor ( GRPR ) is expressed in GABAergic interneurons of the lateral nucleus . ^^^ These experiments provide genetic evidence that GRP and its neural circuitry operate as a negative feedback regulating fear and establish a causal relationship between Grpr gene expression , LTP , and amygdala dependent memory for fear . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
An administration of [ Leu ( 13 ) ( psi CH ( 2 ) NH ) Leu ( 14 ) ] BN ( antagonizes GRP R > neuromedin B receptor ( NMB R ) ) impaired the performance of inhibitory avoidance learning in all doses ( 16 , 32 , 64 nmol / kg ) . ^^^ While the effect was somewhat lesser than [ Leu ( 13 ) ( psi CH ( 2 ) NH ) Leu ( 14 ) ] BN , BIM 23127 ( antagonizes NMB R > GRP R ) also impaired performance in a moderate dose ( 32 nmol / kg ) . ^^^ These results showed that endogenous BN like peptides have some role ( s ) for the modulation of learning and memory , and suggest that NMB / NMB R pathway may also be involved in the memory acquisition and modulation as well as GRP / GRP R pathway . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Here we report cloning and characterization of two subtypes of BN like peptide receptors in Aves . ( 2 ) The amino acid sequence of chick GRP R ( chGRP R ) is highly identical with mammalian and amphibian GRP R , and this receptor showed high affinity for GRP , BN and synthetic bombesin agonist , [ D Phe ( 6 ) , beta Ala ( 11 ) , Phe ( 13 ) , Nle ( 14 ) ] bombesin ( 6 14 ) ( [ FAFNl ] BN ( 6 14 ) ) . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
The mRNA for the major receptor subtype , GRP R , was present in fetal and adult sheep and rat kidneys . ^^^ In the ovine kidney , immunohistochemistry localized GRP predominantly to the thick ascending limb of the loop of Henle . mRNAs for GRP , GRP R , and BRS 3 were detected in the human embryonic kidney cell line HEK 293 , and radioimmunoassay of cell extracts and conditioned media revealed the presence of proGRP but not the amidated form . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
In particular , we have recently found that GRP and its cell surface receptor , GRP R , are abundantly expressed in neuroblastomas . ^^^ Moreover , more advanced stage neuroblastomas demonstrated an increased level of GRP R , suggesting an important role of GRP in aggressive tumor behavior . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
SUMMARY BACKGROUND DATA : We have previously shown that neuroblastomas secrete GRP , which binds to its cell surface receptor ( GRP R ) to stimulate cell growth in an autocrine fashion . ^^^ Human neuroblastoma cell lines ( SK N SH and SH SY5Y ) were stably transfected with the plasmid pEGFP GRP R to establish GRP R overexpression cell lines , and the effects of GRP on PTEN gene and protein expression were determined . ^^^ An increase in GRP binding capacity was confirmed in GRP R overexpressing cells , which demonstrated an accelerated constitutive cell growth rate . ^^^ PTEN gene and protein expression was significantly decreased in GRP R overexpressing cells when compared with controls . ^^^ An increase in GRP binding capacity , as a result of GRP R overexpression , down regulates PTEN expression . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
However , we have suggested that in colon cancer , GRP acts primarily as a morphogen when it and its receptor ( GRP R ) are aberrantly upregulated . ^^^ As such , GRP / GRP R act ( s ) primarily to modulate processes contributing to the assumption or maintenance of tumor differentiation . ^^^ Yet the cellular conditions affecting GRP / GRP R expression , and the biochemical pathways involved in mediating its morphogenic properties , remain to be established . ^^^ We found that confluent cells do not express GRP / GRP R . ^^^ In contrast , disaggreation and plating at subconfluent densities results in rapid GRP / GRP R upregulation followed by their progressive decrease as confluence is achieved . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Receptors for GRP and NMB ( GRP R and NMB R ) , as well as third subtype of bombesin like peptide receptor ( BRS 3 ) have been cloned . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Most leiomyomas ( 20 of 26 ) expressed GRP R but not the leiomyosarcomas . ^^^ The expression of GRP R in glands and vessels during specific phases of the cycle suggests a timely precise physiological action of GRP in these targets ; in certain uterine neoplasms , the GRP R overexpression may contribute to tumor development because GRP is a potent growth factor . ^^^ The expression of GRP R in leiomyomas may allow distinguishing them from receptor negative leiomyosarcomas ; GRP R in leiomyomas , in a subset of endometrial adenocarcinomas , carcinosarcomas , and in peritumoral vessels may be candidates for receptor targeting in vivo . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
ProGRP did not compete with iodinated bombesin in binding assays on Balb 3T3 cells transfected with the known GRP receptors , GRP R or BRS 3 . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Compared with wild type littermates , bombesin treated GRP receptor ( GRPR ) null mice had increased interstitial fibrosis but reduced defects in alveolarization . ^^^ All effects of GRP were abrogated in GRPR null mice . ^^^ GRPR appears to mediate all effects of GRP , but only part of the bombesin effect on alveolarization , suggesting that novel receptors may mediate some effects of bombesin in newborn lung . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
Comparison with GRP / GRP R system will provide important information about BN like peptide systems in mammals . . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
BN receptors on membranes of PC 3 tumors were detected by ( 125 ) 1 [ Tyr ( 4 ) ] BN binding , and expression of mRNA for BRS 3 and GRP R subtypes was also found . ^^^
Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
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Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
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Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
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Interacting proteins: P07492 and P30550	Pubmed	SVM Score :0.0
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Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Of the various types of potent bombesin ( Bn ) / gastrin releasing peptide receptor antagonists that have been discovered , the desMet 14 methyl ester peptides are devoid of residual agonist activity and are among the most potent in terms of in vitro receptor blockade and also in terms of their prolonged inhibition of bombesin stimulated amylase and protein release in the rat . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
PURPOSE : In view of these findings , we investigated the effects of our synthetic bombesin and gastrin releasing peptide receptor antagonist D Tpi 6 , Leu 13 psi ( CH2NH ) Leu 14 bombesin ( 6 14 ) ( RC 3095 ) on the growth of hormone dependent and hormone independent MXT mouse mammary cancers in vivo . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Isolation of a gastrin releasing peptide receptor from normal rat pancreas . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
To determine the pharmacologic and structural properties of the bombesin receptors expressed in human lung carcinoma cells , cDNA clones encoding a human gastrin releasing peptide receptor ( GRP R ) and a pharmacologically distinct neuromedin B preferring bombesin receptor ( NMB R ) were isolated from a human small cell lung carcinoma cell line ( NCI H 345 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Both groups respond similarly to either gastrin releasing peptide or neuromedin B , but only the response to neuromedin B in oocytes expressing the esophagus mRNA is not blocked by a specific gastrin releasing peptide receptor antagonist , des Met [ D Phe 6 ] Bn ( 6 13 ) ethyl ester . ^^^ RNA blot hybridization studies using a Swiss 3T3 gastrin releasing peptide receptor cDNA probe show no detectable hybridization in esophagus mRNA samples . ^^^ These data suggest that a gastrin releasing peptide receptor is expressed in the esophagus and that it is distinct from that expressed in Swiss 3T3 cells and may represent a third subtype of mammalian bombesin receptor . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Properties of detergent solubilized gastrin releasing peptide receptor were investigated . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor is rapidly phosphorylated by a kinase other than protein kinase C after exposure to agonist . ^^^ In this study we show that the gastrin releasing peptide receptor ( GRP R ) is rapidly phosphorylated in response to agonist exposure . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Expression of the gastrin releasing peptide receptor confers a growth response to bombesin in immortalized human bronchial epithelial cells . ^^^ We have introduced a human gastrin releasing peptide receptor expression vector into an immortalized human bronchial epithelial cell normally unresponsive to the ligand bombesin . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Within this region lie the genes for calbindin ( CALB 3 ) , gastrin releasing peptide receptor ( GRPR ) , phosphatidyl inositol glycan class A protein ( PIGA ) , glycine receptor alpha 2 ( GLRA 2 ) , phosphorylase kinase alpha ( PHKA 2 ) , XE 59 ( a gene escaping 10 chromosome inactivation ) , and DXS69E ( 71 7A ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Characterization of gastrin releasing peptide receptor expressed in Sf 9 insect cells by baculovirus . ^^^ In the present study we have expressed the murine gastrin releasing peptide receptor ( mGRP R ) in Sf 9 cells using a recombinant baculovirus and characterized it structurally and functionally . mGRP R was detectible 12 h post infection with recombinant baculovirus carrying mGRP R cDNA and became maximal at 60 h post infection ( Bmax = 6 pmol / mg protein ) , which is a 4 60 fold greater density than is found in native tissues . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Chronic desensitization and down regulation of the gastrin releasing peptide receptor are mediated by a protein kinase C dependent mechanism . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Endocytosis of the gastrin releasing peptide receptor ( GRP R ) may regulate cellular responses to GRP . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Internalization of the gastrin releasing peptide receptor is mediated by both phospholipase C dependent and independent processes . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
In the present study , we investigated the nature and the importance of glycosylation of two mammalian bombesin receptors , the neuromedin B receptor ( NMB R ) and the gastrin releasing peptide receptor ( GRP R ) , using chemical cross linking and enzymatic deglycosylation . [ 125I ] ( D Tyr 0 ) NMB cross linked to native NMB R on rat C 6 glioblastoma cells or rat NMB R transfected into BALB 3T3 cells revealed a single broad band , M ( r ) = 63 , 000 , on both cell types that was not altered by DTT . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Three receptors have been identified for bombesin and its analogs , namely gastrin releasing peptide receptor ( GRPR ) , neuromedin B receptor ( NMBR ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
G alpha q antisense inhibited responses mediated by neuromedin B receptor ( NMB R ) by 74 % , though not by gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The conserved NPXnY motif present in the gastrin releasing peptide receptor is not a general sequestration sequence . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor induced internalization , down regulation , desensitization , and growth : possible role for cyclic AMP . ^^^ Stimulation of the gastrin releasing peptide receptor ( GRP R ) in Swiss 3T3 cells resembles that of a number of other recently described G protein coupled receptors , insofar as both the phospholipase C and adenylyl cyclase signal transduction pathways are activated . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
RESULTS : The pancreatic secretory response to vagal stimulation was blocked significantly by each of the following : the ganglionic blocker hexamethonium ( 100 % inhibition ) ; the muscarinic , cholinergic blocker atropine ( 85 % inhibition ) ; the specific cholecystokinin A receptor antagonist L 364 , 718 ( 84 % inhibition ) ; a gastrin releasing peptide receptor blocker ( 91 % inhibition ) ; and a vasoactive intestinal polypeptide polyclonal antibody ( 89 % inhibition ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Previously , two mammalian BN like peptide receptor subtypes , gastrin releasing peptide receptor and neuromedin B receptor , have been cloned and characterized . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Serines and threonines in the gastrin releasing peptide receptor carboxyl terminus mediate internalization . ^^^ In this study , components of the gastrin releasing peptide receptor ( GRP R ) regulating internalization were identified . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor has been previously cloned from both humans and mice . ^^^ We have mapped the mouse gastrin releasing peptide receptor ( Grpr ) locus using a polymorphic CAn repeat located in the 5 ' untranslated region of the gene and a Mus spretus / Mus musculus interspecific backcross . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We have developed a new immunotherapeutic approach to the treatment of small cell carcinoma of the lung ( SCCL ) by targeting the gastrin releasing peptide receptor ( GRP R ) expressed on the surface of these cells . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Expression of gastrin releasing peptide receptor gene in developing lung . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor signaling resulting in growth inhibition . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Three structurally and pharmacologically distinct bombesin receptor subtypes have been isolated and characterized : the gastrin releasing peptide receptor ( GRP R ) , the neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Agonist binding and protein kinase C activation stimulate phosphorylation of the gastrin releasing peptide receptor at distinct sites . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Expression of gastrin releasing peptide receptor in human skin . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Expression and characterization of gastrin releasing peptide receptor in normal and cancerous pancreas . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Selective reconstitution of gastrin releasing peptide receptor with G alpha q . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Effect of gastrin releasing peptide receptor number on receptor affinity , coupling , degradation , and modulation . ^^^ Three BALB 3T3 cell lines were made that stably expressed the gastrin releasing peptide receptor ( GRP R ) with receptor numbers varying by 280 fold ( GRP R Low , GRP R Med , and GRP R Hi ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor is differentially coupled to adenylate cyclase and phospholipase C in different tissues . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Identification of four amino acids in the gastrin releasing peptide receptor that are required for high affinity agonist binding . ^^^ The bombesin family of G protein coupled receptors includes the gastrin releasing peptide receptor ( GRP R ) , the neuromedin B receptor ( NMB R ) , bombesin receptor subtype 3 ( BRS 3 ) , and bombesin receptor subtype 4 ( bb 4 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Localization of iodine 125 mIP Des Met 14 bombesin ( 7 13 ) NH 2 in ovarian carcinoma induced to express the gastrin releasing peptide receptor by adenoviral vector mediated gene transfer . ^^^ The gastrin releasing peptide receptor ( GRPr ) has a high affinity for the 14 amino acid bombesin peptide . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The translocation breakpoint on the 10 chromosome occurred in the first intron of the gastrin releasing peptide receptor ( GRPR ) gene and that on chromosome 8 occurred approximately 30 kb distal to the 3 ' end of the Syndecan 2 gene ( SDC 2 ) , also known as human heparan sulfate proteoglycan or fibroglycan . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Expression of mRNA for gastrin releasing peptide receptor by human bronchial epithelial cells . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
To elucidate the molecular basis underlying this selectivity , different Gq / 11 coupled receptors ( m 3 muscarinic , V1a vasopressin , and gastrin releasing peptide receptor ) were coexpressed ( in COS 7 cells ) with mutant alphas subunits in which residues present at the C terminus of alphas were replaced with the corresponding alphaq / 11 residues . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Generation and characterization of mice lacking gastrin releasing peptide receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Constitutive activation of the gastrin releasing peptide receptor expressed by the nonmalignant human colon epithelial cell line NCM 460 . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Bombesin like peptide receptors cloned so far include , gastrin releasing peptide receptor ( GRP R ) , neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Construction of chimeric human bombesin receptors to identify neuromedin B and gastrin releasing peptide receptor binding sites . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Tumor localization of a radiolabeled bombesin analogue in mice bearing human ovarian tumors induced to express the gastrin releasing peptide receptor by an adenoviral vector . ^^^ BACKGROUND : The adenoviral vector , AdCMVGRPr , has been used to induce the expression of the murine gastrin releasing peptide receptor ( GRPr ) both in vitro and in vivo . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Activation of c Jun N terminal kinase is dependent on the expression of the gastrin releasing peptide receptor in rat 1A fibroblasts , demonstrating that the responses to the peptide are receptor mediated . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The subsequent identification of YAC positive clones from this region allowed us to refine the map and localize FIGF centromeric to the phosphatidylinositol glycan complementation class A ( PIGA ) gene and telomeric to the gastrin releasing peptide receptor ( GRPR ) gene . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
In situ hybridization for gastrin releasing peptide receptor ( GRP receptor ) expression in prostatic carcinoma . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Loss of bombesin induced feeding suppression in gastrin releasing peptide receptor deficient mice . ^^^ The gastrin releasing peptide receptor ( GRP R ) is one of three members of the mammalian bombesin subfamily of seven transmembrane G protein coupled receptors that mediate diverse biological responses including secretion , neuromodulation , chemotaxis , and growth . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The mammalian bombesin ( Bn ) like neuropeptide receptors gastrin releasing peptide receptor ( GRP R ) and neuromedin B receptor ( NMB R ) transduce a variety of physiological signals that regulate secretion , growth , muscle contraction , chemotaxis and neuromodulation . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Four subtypes of bombesin receptors are identified ( gastrin releasing peptide receptor , neuromedin B receptor , the orphan receptor bombesin receptor subtype 3 ( BB 3 or BRS 3 ) and bombesin receptor subtype 4 ( BB 4 ) ) , however , only the pharmacology of the gastrin releasing peptide receptor has been well studied . ^^^ In rat pancreatic acini containing only gastrin releasing peptide receptor and in BB 4 transfected BALB cells , this ligand and 125I [ Tyr 4 ] bombesin , the conventional gastrin releasing peptide receptor ligand , gave similar results for receptor number , affinity for bombesin and affinity for the unlabeled ligand . ^^^ The unlabeled ligand was an agonist only at the gastrin releasing peptide receptor in rat acini and did not interact with CCK ( A ) receptors or muscarinic M 3 acetylcholine receptors to increase [ 3H ] inositol phosphates . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
This family of heptahelical , G protein coupled receptors includes the gastrin releasing peptide receptor ( GRP R , or bb 2 ) , neuromedin B receptor ( NMB R , or bb 1 ) , and the bombesin receptor subtype 3 ( BRS 3 , or bb 3 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Three mammalian bombesin receptor subtypes have been characterized : the gastrin releasing peptide receptor ( GRP R ) , the neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The effects of a potent specific gastrin releasing peptide receptor antagonist , BIM 26226 ( [ D F 5 Phe6 , D Ala 11 ] bombesin ( 6 13 ) OMe ) , and the long acting somatostatin analogue , lanreotide ( BIM 23014 ) , on the growth of an acinar pancreatic adenocarcinoma growing in the rat or cultured in vitro were investigated . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Exclusion of the gastrin releasing peptide receptor ( GRPR ) locus as a candidate gene for Rett syndrome . ^^^ The gene for the gastrin releasing peptide receptor ( GRPR ) has been mapped to a candidate region for Rett syndrome ( RTT ) on the short arm of the 10 chromosome . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Similar experiments with the expressed gastrin releasing peptide receptor and muscarinic m 1 receptor revealed the coupling of Galphaq and Galpha 11 , but not Galpha 14 , to these receptors in oocytes . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The bombesin ( Bn ) / gastrin releasing peptide receptor GRP R , which is coupled to phospholipase C via the Gq family of transducing proteins , is internalized rapidly after Bn binding . ^^^ Role of receptor and protein kinase C activation in the internalization of the gastrin releasing peptide receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Adenoviral mediated delivery of gastrin releasing peptide receptor results in specific tumor localization of a bombesin analogue in vivo . ^^^ In this regard , a radiolabeled bombesin analogue was used in conjunction with a recombinant adenoviral vector encoding the murine gastrin releasing peptide receptor ( mGRPr ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Mice administered a control adenoviral vector encoding the gastrin releasing peptide receptor did not have tumor localization of [ ( 111 ) In ] DTPA D Phe 1 octreotide ( < 1 . 6 % ID / g ) , demonstrating specificity of [ ( 111 ) In ] DTPA D Phe 1 octreotide for the AdCMVhSSTr 2 induced tumor cells . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We used an in situ reconstitution assay to examine the receptor coupling to purified G protein alpha subunits by the bombesin receptor family , including gastrin releasing peptide receptor ( GRP R ) , neuromedin B receptor ( NMB R ) , and bombesin receptor subtype 3 ( BRS 3 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
High gastrin releasing peptide receptor mRNA level is related to tumour dedifferentiation and lymphatic vessel invasion in human colon cancer . ^^^ Through pharmacological testing , 20 40 % of human colorectal tumours have been shown to be equipped with bombesin / gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The 14 aminoacid peptide bombesin ( BN ) has a high affinity for the gastrin releasing peptide receptor which is expressed by a variety of tumours . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
To this end , an adenoviral vector ( AdCMVGRPr ) encoding the murine gastrin releasing peptide receptor ( GRPr ) was used to achieve a high level of binding of radiolabeled bombesin ( BBN ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
An aspartate residue at the extracellular boundary of TMII and an arginine residue in TMVII of the gastrin releasing peptide receptor interact to facilitate heterotrimeric G protein coupling . ^^^ The mammalian bombesin receptor subfamily of G protein coupled receptors currently consists of the gastrin releasing peptide receptor ( GRP R ) , neuromedin B receptor , and bombesin receptor subtype 3 . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Because it had been shown previously that either synthetic analogs of bombesin ( Bn ) or other receptor peptoid or receptor antagonists function as an antagonist or agonist depends on animal species and receptor subtype studied , we investigated the pharmacological properties of PD 168368 compared with all currently known Bn receptor subtypes ( NMB receptor , gastrin releasing peptide receptor , Bn receptor subtype 3 , and Bn receptor subtype 4 ) from human , mouse , rat , and frog . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Bombesin ( BN ) is a 14 amino acid neuropeptide with a high affinity for the gastrin releasing peptide receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Phosphorylation uncouples the gastrin releasing peptide receptor from G ( q ) . ^^^ In this study we demonstrate that for the G ( q ) coupled gastrin releasing peptide receptor ( GRP R ) , phosphorylation by GRK 2 to a stoichiometry of approximately 1 mol PO ( 4 ) / mol GRP R is sufficient in the absence of arrestin to reduce the rate of receptor catalyzed G protein activation by approximately 80 % . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Sex specific expression of gastrin releasing peptide receptor : relationship to smoking history and risk of lung cancer . ^^^ BACKGROUND : Activation of gastrin releasing peptide receptor ( GRPR ) in human airways has been associated with a proliferative response of bronchial cells to gastrin releasing peptide and with long term tobacco use . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Three bombesin receptor subtypes , termed gastrin releasing peptide receptor ( GRPR ) , neuromedin B receptor ( NMBR ) , and bombesin receptor subtype 3 ( BRS 3 ) , have been identified in rodents and humans . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Molecular organization of the mouse gastrin releasing peptide receptor gene and its promoter . ^^^ The murine gastrin releasing peptide receptor ( mGRP R ) is a member of the G protein coupled receptor family and mediates important physiological actions of its specific ligand , the gastrointestinal hormone / neurotransmitter GRP , including mitogenic properties in the mouse Swiss 3T3 fibroblasts . ^^^ We isolated and characterized genomic bacteriophage P 1 clones encoding the mouse gastrin releasing peptide receptor ( mGRP R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
METHODS : A total of 26 human pancreatic adenocarcinomas , obtained after resection , and 5 pancreatic cancer xenografts were analyzed for expression of NTR , vasoactive intestinal peptide receptor ( VIPR ) , substance P receptor ( SPR ) , and gastrin releasing peptide receptor ( GRPR ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
In this study , we used the reverse transcription polymerase chain reaction ( RT PCR ) method to evaluate the mRNA expression of three bombesin receptor subtypes : gastrin releasing peptide receptor ( GRPR ) , neuromedin B receptor ( NMBR ) , and bombesin receptor subtype 3 ( BRS 3 ) , in 22 specimens of human epithelial ovarian cancer and in two human ovarian cancer lines . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Male mice lacking the gastrin releasing peptide receptor ( GRP R ) display elevated preference for conspecific odors and increased social investigatory behaviors . ^^^ Previously , we generated gastrin releasing peptide receptor null mutant mice ( GRP R deficient mice ) , and found that these animals displayed increased non aggressive social responses in an ordinary social interaction test using a resident intruder method . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Two amino acids in the sixth transmembrane segment of the mouse gastrin releasing peptide receptor are important for receptor activation . ^^^ The gastrin releasing peptide receptor ( GRP R ) is a G protein coupled receptor that mediates a variety of cellular responses , including cell growth and modulation of neuronal activity by activation of heterotrimeric GTP binding proteins in the Gq family . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The broad spectrum antagonist of neuropeptide receptor , [ D Arg 1 , D Phe 5 , D Trp 7 , 9 , Leu 11 ] substance P , induced apoptosis selectively in human small cell lung carcinoma ( SCLC ) cells , which express gastrin releasing peptide receptor , but not in other types of tumor cells as well as normal cells . ^^^ Thus , blockage of gastrin releasing peptide receptor mediated signaling may provide a novel therapeutic option in SCLC which has become resistant to conventional chemotherapeutic agents . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Glycosylation of the gastrin releasing peptide receptor and its effect on expression , G protein coupling , and receptor modulatory processes . ^^^ We used site directed mutagenesis to address these issues with the gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Adenovirus vectors encoding the genes for a variety of antigens / receptors ( carcinoembryonic antigen , gastrin releasing peptide receptor , somatostatin receptor subtype 2 ( SSTr 2 ) ) have all shown that their expression is increased on cancer cells both in vitro and in vivo following adenovirus infection . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
In order to specify these functions , our group and another laboratory generated by gene targeting mutant mice that lack one of the three BN like peptide receptors found in mammals , ie neuromedin B receptor ( NMB R ; BB 1 ) , gastrin releasing peptide receptor ( GRP R ; BB 2 ) , or bombesin receptor subtype 3 ( BRS 3 ; BB 3 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The receptor mediating the action of the mammalian bombesin ( Bn ) peptide , gastrin releasing peptide receptor ( GRPR ) , is an exception , because numerous classes of peptide antagonists are described . ^^^ Molecular basis for selectivity of high affinity peptide antagonists for the gastrin releasing peptide receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We assessed the role of BN like peptides / receptors in emotional and / or anxiety related behavior using three strains of knockout mice , each deficient in a single BN like peptide receptor ( gastrin releasing peptide receptor , bombesin receptor subtype 3 , or neuromedin B receptor ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor mediated autocrine growth in squamous cell carcinoma of the head and neck . ^^^ BACKGROUND : Gastrin releasing peptide receptor ( GRPR ) mediated autocrine growth appears to be an early marker of susceptibility to tobacco related lung cancers . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Inhibition of proliferation of human small cell lung cancer cells expressing an autocrine system for gastrin releasing peptide by antisense oligodeoxynucleotides to gastrin releasing peptide receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Regulation of gastric function by endogenous gastrin releasing peptide in humans : studies with a specific gastrin releasing peptide receptor antagonist . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Molecular basis of the selectivity of gastrin releasing peptide receptor for gastrin releasing peptide . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Disruptions in feeding and body weight control in gastrin releasing peptide receptor deficient mice . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The orphan receptor , human bombesin receptor subtype 3 ( BRS 3 ) was assigned to the G protein coupled bombesin receptor family because of its high sequence homology with the neuromedin B receptor ( NMB R ) and gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Human gastrin releasing peptide receptor mediates sustained CREB phosphorylation and transactivation in HuTu 80 duodenal cancer cells . ^^^ The G protein coupled human gastrin releasing peptide receptor ( hGRP R ) is frequently found aberrantly expressed in human cancers of the colon , stomach , and lung , and its ligand specific activation has been implicated in cell proliferation and differentiation . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Islet function phenotype in gastrin releasing peptide receptor gene deficient mice . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Expression of progastrin releasing peptide and gastrin releasing peptide receptor mRNA transcripts in tumor cells of patients with small cell lung cancer . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
METHODS : Expression of prepro gastrin releasing peptide ( preproGRP ) , neuromedin B receptor ( NMB R ) and gastrin releasing peptide receptor ( GRP R ) were examined by reverse transcriptase polymerase chain reaction ( RT PCR ) in peripheral blood and bone marrow aspirate from 40 untreated patients with SCLC . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Increased frequency of gastrin releasing peptide receptor gene mutations during colon adenocarcinoma progression . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
A recessive deafness mutation in the mouse arose spontaneously and was identified in a colony segregating a null allele of the gastrin releasing peptide receptor ( Grpr ) locus . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We analyzed murine mast cells for BLP receptor gene expression , and identified mRNAs encoding bombesin receptor subtype 3 and neuromedin B receptor ( NMB R ) , but not gastrin releasing peptide receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
MicroPET imaging of a gastrin releasing peptide receptor positive tumor in a mouse model of human prostate cancer using a 64Cu labeled bombesin analogue . ^^^ The gastrin releasing peptide receptor ( GRPR ) is overexpressed on a variety of carcinomas and has been the target for detection and treatment of these neoplasms in animals . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We found , in this study , bombesin specifically induced mitogen activated protein ( MAP ) kinase activation as shown by increased extracellular regulated kinase ( ERK ) phosphorylation and epidermal growth factor ( EGF ) receptor transactivation in prostate cancer cells , which express functional gastrin releasing peptide receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Nonpeptide gastrin releasing peptide receptor antagonists inhibit the proliferation of lung cancer cells . ^^^ These results indicate that PD 176252 is a gastrin releasing peptide receptor antagonist , which inhibits the proliferation of lung cancer cells . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Importance of amino acids of the central portion of the second intracellular loop of the gastrin releasing Peptide receptor for phospholipase C activation , internalization , and chronic down regulation . ^^^ We used site directed mutagenesis to address these issues with the gastrin releasing peptide receptor ( GRP R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Agonist and protein kinase C induced phosphorylation have similar functional consequences for gastrin releasing peptide receptor signaling via Gq . ^^^ Gastrin releasing peptide receptor ( GRPr ) is phosphorylated by a kinase other than protein kinase C ( PKC ) after exposure to agonist and is also a substrate for PKC dependent phosphorylation after treatment with 12 O tetradecanoylphorbol 13 acetate ( TPA ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor ( GRPR ) locus in Japanese subjects with autism . ^^^ Gastrin releasing peptide receptor ( GRPR ) gene is considered a candidate locus for infantile autism for several reasons . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
This approach was termed `` Genetic Radioisotope Targeting Strategy . ' ' Using this strategy , induction of high levels of gastrin releasing peptide receptor or human somatostatin receptor subtype 2 expression and selective tumor uptake of radiolabeled peptides was achieved . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Transcriptional activation of the human gastrin releasing peptide receptor gene in gastrointestinal and prostatic epithelial cancer cells . ^^^ The mammalian gastrin releasing peptide receptor ( GRP R ) belongs to the superfamily of G protein coupled receptors and mediates actions of the regulatory GRP and bombesin , the amphibian homolog of GRP . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide and gastrin releasing peptide receptor expression in gastrointestinal carcinoid tumours . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Ets transcriptional regulation of gastrin releasing peptide receptor in neuroblastomas . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Alterations in receptor expression or agonist concentration change the pathways gastrin releasing peptide receptor uses to regulate extracellular signal regulated kinase . ^^^ In this study , we demonstrate that gastrin releasing peptide receptor ( GRPr ) regulates ERK through multiple pathways in a single cell type depending upon receptor expression and agonist concentration . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Dexamethasone regulation of gastrin releasing peptide receptor in human lung cells . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor expression is decreased in patients with Crohn ' s disease but not in ulcerative colitis . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We therefore investigated the effect of bombesin ( a homologue of gastrin releasing peptide ) on proliferation , migration and inositol phosphate production in the human colorectal carcinoma cell line DLD 1 , and determined the ability of gastrin releasing peptide receptor antagonists to inhibit these effects . ^^^ We conclude that both amidated and non amidated forms of gastrin releasing peptide accelerate proliferation and migration of DLD 1 human colorectal carcinoma cells via the gastrin releasing peptide receptor , but that phosphatidylinositol 3 kinase is only involved in the cell migration signalling pathway . ^^^ Our results suggest a potential role for gastrin releasing peptide receptor antagonists in the management of colorectal carcinoma . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Peptides showed high affinity for the human GRP R ( GRP R = gastrin releasing peptide receptor ) expressed in PC 3 cells . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Identification of key amino acids in the gastrin releasing peptide receptor ( GRPR ) responsible for high affinity binding of gastrin releasing peptide ( GRP ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Immunohistochemical localization of gastrin releasing peptide receptor in the mouse brain . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Bombesin ( BN ) , a 14 amino acid peptide , shows high affinity for the human gastrin releasing peptide receptor ( GRP r ) , which is overexpressed on several types of cancer , including prostate , breast , gastrointestinal , and small cell lung cancer . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The biologic profiles of [ ( 99m ) Tc ] Demobesin 1 ( [ ( 99m ) Tc N ( 4 ) ( 0 1 ) , bzlg ( 0 ) , d Phe ( 6 ) , Leu NHEt ( 13 ) , des Met ( 14 ) ] BB ( 6 14 ) ) and [ ( 111 ) In ] Z 070 were compared using various gastrin releasing peptide receptor GRP R ) expressing tissues of human and animal origin . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Expression of gastrin releasing Peptide receptor in renal cell carcinomas : a potential function for the regulation of neoangiogenesis and microvascular perfusion . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor mediates activation of the epidermal growth factor receptor in lung cancer cells . ^^^ Gastrin releasing peptide receptor ( GRPR ) and the epidermal growth factor receptor ( EGFR ) are expressed in several cancers including non small cell lung cancer ( NSCLC ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Non associative learning and anxiety in rats treated with a single systemic administration of the gastrin releasing peptide receptor antagonist RC 3095 . ^^^ The gastrin releasing peptide receptor ( GRPR ) has been implicated in the modulation of emotionally motivated memory . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Bombesin is a 14 amino acid peptide with very high affinity for the BB 2 or gastrin releasing peptide receptor ( GRPr ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Overexpression of gastrin releasing peptide receptor induced layer disorganization in brain . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor antagonist effects on an animal model of sepsis . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The gastrin releasing peptide receptor ( GRPR ) is found to be overexpressed in a variety of human tumors . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Diurnal regulation of the gastrin releasing peptide receptor in the mouse circadian clock . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptor as a molecular target for psychiatric and neurological disorders . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Opposite effects of low and high doses of the gastrin releasing peptide receptor antagonist RC 3095 on memory consolidation in the hippocampus : possible involvement of the GABAergic system . ^^^ Although the gastrin releasing peptide receptor ( GRPR ) has recently emerged as a system importantly involved in regulating memory formation , the role of hippocampal GRPRs in memory remains controversial . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Progastrin releasing peptide and gastrin releasing peptide receptor mRNA expression in non tumor tissues of the human gastrointestinal tract . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Some of them ( namely potassium competitive acid blockers ( P CABs ) and CCK 2 receptor antagonists ) have already reached clinical testing while some others ( like the antigastrin vaccine , H 3 receptor ligands or gastrin releasing peptide receptor antagonists ) are still in preclinical development and need the proof of concept in human beings . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The focus of this study was to evaluate the therapeutic benefit of combined gastrin releasing peptide ( GRP ) receptor targeted radiotherapy ( TRT ) with chemotherapy , using the PC 3 xenograft severe combined immunodeficiency ( SCID ) mouse model . ( 177 ) Lu DOTA 8 AOC BBN ( 7 14 ) NH ( 2 ) is a radiotherapeutic peptide that specifically targets the gastrin releasing peptide receptor overexpressed on primary and metastatic prostate cancer . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Although the gastrin releasing peptide receptor has been implicated in memory consolidation , previous studies have not examined whether it is involved in extinction . ^^^ Here we show that gastrin releasing peptide receptor blockade in the hippocampus disrupts extinction of aversive memory . ^^^ Infusion of a gastrin releasing peptide receptor antagonist or the protein synthesis inhibitor anisomycin into the dorsal hippocampus immediately after the first extinction session blocked extinction . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Consequence of gastrin releasing peptide receptor activation in a human colon cancer cell line : a proteomic approach . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The results of these investigations demonstrate the ability of these new conjugates to specifically target the gastrin releasing peptide receptor subtype 2 , which is overexpressed on human prostate PC 3 cancerous tissues . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We describe the structure and expression of a cloned NMB preferring bombesin receptor ( NMB R ) with properties distinct from a GRP preferring bombesin receptor ( GRP R ) reported previously . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
GRP preferring bombesin receptor subtype mediates contractile activity in cat terminal ileum . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
In human lung membranes , bombesin , gastrin releasing peptide ( GRP ) and GRP preferring bombesin receptor antagonists displaced [ 125I Tyr 4 ] bombesin binding with high affinities ( 36 177 nM ) , whereas neuromedin B possessed a lower affinity of 2878 nM . [ D F5Phe6 , D Ala 11 ] bombesin ( 6 13 ) methyl ester , the most active GRP preferring bombesin antagonist as yet reported , had the highest affinity among all antagonists tested whereas neuromedin B had the lowest affinity . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
For the naturally occurring agonists , at the GRP preferring bombesin receptor the relative affinities were litorin = ranatensin = bombesin > GRP > > neuromedin B , phyllolitorin and at the neuromedin B preferring bombesin receptor were litorin = neuromedin B = ranatensin > bombesin , phyllolitorin > > GRP . ^^^ For the GRP preferring bombesin receptor the heptapeptide and for the neuromedin B preferring bombesin receptor the octapeptide was the minimal carboxyl fragment interacting with the receptor / or causing biologic activity , and the nonapeptide and full decapeptide , respectively , were the minimal required for full affinity . ^^^ A conformationally restricted GRP analogue , [ D Cys 6 , D Ala 11 , Cys 14 ] bombesin ( 6 14 ) had a significantly higher affinity for GRP preferring bombesin receptor than NMB receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The sublibraries with the highest affinity to the MSH receptor ( i . e . melanocortin type 1 or MC 1 receptor ) and , respectively , the GRP preferring bombesin receptor were identified by an iterative process . ^^^ The KD of the most potent MC 1 receptor ligand was 1 . 58 mumol / l and that of the GRP preferring bombesin receptor 3 . 40 mumol / l . ^^^ These results demonstrate that testing a peptoid library containing 328 , 509 single compounds led to the successful identification of new ligands for both the MC 1 receptor as well as the GRP preferring bombesin receptor . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Ligand binding studies show that while both receptors can be activated by either GRP or NMB , one receptor has a higher affinity for GRP than for NMB ( GRP R ) , while the other has a higher affinity for NMB than for GRP ( NMB R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Mammalian bombesin like peptides ( gastrin releasing peptide [ GRP ] and neuromedin B [ NMB ] ) and their receptors ( GRP R and NMB R ) can stimulate growth of cultured cells , and have been shown to be part of an autocrine growth regulatory network in some human small cell lung carcinoma cells . ^^^ As a first step , we examined the distribution of expression of GRP R and NMB R mRNA during rat embryonic development to identify changing spatio temporal patterns of gene expression . ^^^ In situ hybridization studies show that GRP R mRNA is expressed at early embryonic stages in various locations of the nervous , urogenital , respiratory , and gastrointestinal systems . ^^^ However , transient GRP R mRNA expression is observed in the posterior pituitary where expression increases from embryonic day 12 to 20 , and abruptly disappears at birth . ^^^ These studies suggest that appropriate development of several organ systems , in particular the posterior pituitary , may involve GRP R mediated signaling . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
In total RNA from fresh AP we detected high amounts of NMB mRNA and much smaller amounts of GRP mRNA , while finding a weak signal for GRP receptor ( GRP R ) and NMB receptor ( NMB R ) mRNAs . ^^^ In total RNA from the reaggregate cell cultures we detected high levels of NMB mRNA as well as a strong signal for GRP R mRNA . ^^^ Finally , in GH 3 cells , high levels of NMB mRNA and GRP R mRNA were found , while GRP mRNA and NMB R mRNA remained undetectable even in high amounts ( 200 micrograms ) of total RNA . ^^^ NMB R mRNA and GRP R mRNA are both present in low levels in fresh AP whereas the GRP R mRNA is predominant in GH 3 cells and estradiol treated AP reaggregate cell cultures . ^^^ Compared to fresh AP tissue , NMB mRNA and GRP R mRNA expression is enhanced in estradiol treated reaggregate cell cultures . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : We used digoxigenin labeled cRNA probe for GRP and a biotinylated cRNA probe for GRP receptor ( GRP R ) on frozen and / or formalin fixed , paraffin embedded sections of rat brain . ^^^ Antidigoxigenin alkaline phosphatase conjugate and nitro blue tetrazolium , producing a blue color reaction , was used to visualize GRP mRNA , and for GRP R mRNA , avidin alkaline phosphatase and vector red , were used resulting in a vivid red color . ^^^ RESULTS : Strong specific signals for GRP and / or GRP R were detected in rat brain neurons . ^^^ The overall distribution of GRP and GRP R mRNAs in rat brain was similar to that previously reported with single ISH using radioactive labeled probes . ^^^ Double ISH revealed colocalization of the two mRNAs in some neurons with the expression of GRP mRNA often coexpressed with GRP R mRNA . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Recent cloning studies confirm two subtypes of Bn receptors exist , a neuromedin B preferring receptor ( NMB R ) and a gastrin releasing peptide preferring receptor ( GRP R ) . ^^^ The rat glioblastoma C 6 cell line which possess functional NMB R and 3T3 cells which possess functional GRP R were used . 125I [ D Tyr 0 ] NMB and 125I [ Tyr 4 ] Bn were prepared using Iodogen and purified on HPLC . ^^^ At 37 degrees C binding of 125I [ D Tyr 0 ] NMB to NMB R or 125I [ Tyr 4 ] Bn to GRP R was maximal by 5 15 min and decreased to 60 70 % after 60 min . ^^^ Both subtypes occur widely in GI tract and the CNS ; however , in contrast to the GRP R subtype little is known about the ligand receptor interactions for the NMB R . ^^^ Therefore , in the present study we explored the ligand receptor interactions including kinetics , stoichiometry , internalization , degradation and regulation by guanine nucleotide binding proteins with the NMB R and compared it to the GRP R . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The specific GRP R antagonist blocked the response to bombesin in oocytes injected with GRP R , but failed to do so in oocytes injected with NMB R . ^^^ Somatic cell hybrids localized the GRP R on the 10 chromosome and the NMB R on chromosome 6 . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The two bombesin receptor subtypes , neuromedin B ( NMB R ) and gastrin releasing peptide ( GRP R ) receptors , bind their respective ligands with high affinity . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The purpose of our study was to ( 1 ) confirm functional GRP R in SIIA and ( 2 ) determine whether bombesin alters the expression and binding activity of the AP 1 transcription factors , c jun and jun B . ^^^ RESULTS : SIIA cells mobilized intracellular calcium in response to bombesin , exhibiting a functional cell surface GRP R . ^^^ CONCLUSIONS : The SIIA human gastric cancer possesses functional GRP R coupled to the calcium second messenger pathway . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
To determine which of the three known bombesin receptor subtypes ( GRP receptor ( GRP R ) , neuromedin B receptor , bombesin receptor subtype 3 ) were expressed in the cell lines , reverse transcription / PCR analysis of cellular RNA followed by hybridization with receptor specific cDNA was performed . ^^^ This revealed the presence of GRP R transcript in all cell lines , while neither of the other two receptor transcripts were expressed . ^^^ Our data indicate that bombesin is a potent inducer of signal transduction via GRP R receptors in androgen insensitive PC 3 and DU 145 prostate cancer cells . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Therefore , we examined the uterus and placenta of pregnant women at term for synthesis of GRP and expression of GRP receptor genes BRS 3 and GRP R . ^^^ Minor peaks were also observed : two larger than the main form and one corresponding to GRP ( 18 27 ) . mRNA for GRP receptors GRP R and BRS 3 was detected by semiquantitative reverse transcription PCR . ^^^ GRP R mRNA predominated in the pregnant endometrium , whereas BRS 3 mRNA predominated in the membranes and placenta . ^^^ The detection of mRNA for GRP R , BRS 3 , and possibly a transcript variant of BRS 3 as well as the detection of a peptide larger than , but related to , GRP suggest a novel regulatory unit in the human reproductive tract . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The mechanism is not known , and a potent and specific GRP R antagonist BIM 26226 , which has low affinity for NMB R , was used in vivo in athymic nude mice bearing gastrinoma subcutaneously . ^^^ RT PCR study of gastrinoma revealed the presence of both GRP R and NMB R mRNA , but much more abundant NMB R mRNA . ^^^ We conclude that BBS stimulated growth of gastrinoma involves both GRP R and NMB R , and our findings suggest that GRP R mediates negative and NMB R produces positive growth effects on gastrinoma . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We obtained endoscopic pinch biopsies of , and extracted the RNA from , epithelial cells lining the esophagus , stomach , jejunum , ileum , and proximal and descending colon , RT PCR demonstrated that GRP R expression is limited to cells lining the gastric antrum , indicating that this receptor is aberrantly expressed by GI cancers . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We determined whether a human gastric cancer cell line ( SIIA ) expresses a functional GRP receptor ( GRP R ) . ^^^ BBS increased intracellular calcium ( [ Ca2+ ] 1 ) , and a specific GRP R antagonist , ( [ D Phe 6 , Des Met 14 ] BBS ( 6 14 ) ethylamide ) , blocked BBS induced increase in [ Ca2+ ] 1 . ^^^ SIIA cells possess GRP R mRNA by reverse transcriptase PCR . ^^^ These findings indicate that SIIA cells possess a GRP R that is capable of physiological signal transduction , though the cellular response remains unknown . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The purpose of our present study was to determine : ( 1 ) whether the gastrin gene and peptide could be detected in ovarian cancer cell lines , ( 2 ) if functional gastrin releasing peptide receptors ( GRP R ) are present , and ( 3 ) whether gastrin gene expression is altered by GRP . ^^^ SW 626 cells express a functional GRP R that is correctly coupled to the Ca2+ signaling pathway . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
To determine if the expression of bombesin like peptides ( BLP ) and their receptors ( GRP R and NMB R ) correlate with an in vitro response to 2A11 , we measured these parameters in seven SCLC cell lines . ^^^ Calcium mobilization in response to BLP stimulation was detected in the six cell lines expressing either GRP R or NMB R mRNA but not in NCI N 417 , which had no detectable BLP receptor . 2A11 ( 5 microg / ml ) inhibited colony formation by 26 61 % after 2 weeks in all cell lines except NCI N 417 . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Previously , we have generated and characterized mice with targeted disruption of the two other BN like peptide receptors , bombesin receptor subtype 3 ( BRS 3 ) and gastrin releasing peptide preferring receptor ( GRP R ) . ^^^ Here we describe the generation and analysis of NMB R deficient mice to investigate how NMB R differs from BRS 3 and GRP R . ^^^ Compensation for NMB R deficiency by overexpression of GRP R and / or BRS 3 was not detected . ^^^ In addition , the behavioral phenotypes of GRP R deficient mice were not observed in NMB R deficient mice . ^^^ These data show that the functions of NMB R and GRP R are distinct , with only partial overlap . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The pathophysiological relevance of the bombesin / GRP receptor ( GRP R ) , which is expressed in 30 % of human colon tumor cell lines and in 24 40 % of native tumors , has not been clearly assessed at this time . ^^^ Competitive reverse transcription PCR showed a high GRP R mRNA level in Isreco 1 cells , and binding studies confirmed the expression of bombesin / GRP subtype receptors ( Kd = 0 . 42 nM ; Bmax = 18 , 000 sites / cell ) . ^^^ These results clearly indicate that bombesin exerts morphological , adhesive , and proliferative effects on Isreco 1 cells , suggesting that expression of the bombesin / GRP R may contribute to the malignant properties of colon carcinoma cells . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Epithelial cells lining the GI tract except in the gastric antrum do not normally express gastrin releasing peptide receptors ( GRP R ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We determined the roles of intracellular free Ca ( 2+ ) ( [ Ca ( 2+ ) ] ( 1 ) ) , protein kinase C ( PKC ) , and mitogen activated protein kinases ( MAPK ) in GRP receptor ( GRP R ) regulated secretion . ^^^ GRP R regulated secretion is initiated by an increase in [ Ca ( 2+ ) ] ( 1 ) ; however , elevated [ Ca ( 2+ ) ] ( 1 ) is insufficient to stimulate secretion in the absence of activation of PKC and the downstream MEK / MAPK pathways . ^^^ We demonstrated that the activity of MEK is important for maintaining elevated [ Ca ( 2+ ) ] ( 1 ) levels induced by GRP R activation , suggesting that MEK may affect receptor regulated secretion by modulating the activity of Ca ( 2+ ) sensitive PKC . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The PAMP induced hyperglycemic effect was inhibited by [ D Phe ( 6 ) , Leu NHEt ( 13 ) , des Met ( 14 ) ] BN ( 6 14 ) , GRP R specific antagonist , indicating that the hyperglycemic effect is mediated at least in part via GRP R . ^^^ PAMP showed affinity for gastrin releasing peptide preferring receptor ( GRP R ) and neuromedin B preferring receptor . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Comparison with GRP / GRP R system will provide important information about BN like peptide systems in mammals . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The hormone bombesin ( BBS ) and its mammalian equivalent gastrin releasing peptide ( GRP ) act through specific GRP receptors ( GRP R ) to affect multiple cellular functions in the gastrointestinal tract ; the intracellular signaling pathways leading to these effects are not clearly defined . ^^^ Previously , we demonstrated that the human gastric cancer SIIA possesses GRP R and that BBS stimulates activator protein 1 ( AP 1 ) gene expression . ^^^ A rapid induction of c jun and jun B gene expression was noted after BBS treatment ; this effect was blocked by specific GRP R antagonists , indicating that BBS is acting through the GRP R . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
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Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
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Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
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Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
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Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.51454974
These studies demonstrate that BN analogues can be conjugated with PEG linkers , radiolabeled with 64Cu , and bind to GRPR . 0.51454974^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Although epithelial cells lining the mouse colon do not normally express GRP and its receptor ( GRP R ) , both are aberrantly expressed by all better differentiated cancers in wild type C57BL / 6J mice treated with the carcinogen azoxymethane . ^^^ Whereas small tumors in both wild type and GRP R deficient ( i . e . , GRP R / ) mice are histologically similar , larger tumors become better differentiated in the former but degenerate into more poorly differentiated mucinous adenocarcinomas in the latter . ^^^ This alteration in phenotype is attributable to GRP increasing focal adhesion kinase expression in GRP R expressing tumors . ^^^ Consistent with GRP acting as a mitogen , GRP / GRP R coexpressing tumors in wild type animals also contain more proliferating cells than those occurring in GRP R / mice . ^^^ Yet tumors are similarly sized in animals of either genotype receiving azoxymethane for identical times , a finding attributable to the significantly higher number of apoptotic cells detected in GRP / GRP R coexpressing cancers . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Although epithelial cells lining the colon do not normally express GRP or its receptor ( GRP R ) , most human tumors express GRP R mRNA . ^^^ To elucidate the reason for the difference between the expression of GRP / GRP R mRNA and protein , we studied nine human colon cancer cell lines . ^^^ Quantitative polymerase chain reaction revealed that all colon cancer cell lines expressed similar amounts of mRNA for both GRP as well as GRP R . ^^^ Conformational fragment length polymorphism analysis indicated that although mRNA for the ligand GRP was never mutated , mRNA for the GRP R was always mutated . ^^^ Sequencing revealed that the message for GRP R contained between two and seven separate mutations at the nucleotide level . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
BN receptors on membranes of PC 3 tumors were detected by ( 125 ) 1 [ Tyr ( 4 ) ] BN binding , and expression of mRNA for BRS 3 and GRP R subtypes was also found . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We now investigate gene expression and function of BLP ( gastrin releasing peptide , GRP ) and BLP receptors ( GRP R and BRS 3 ) in lung from two baboon BPD models . ^^^ In the `` hyperoxic model , ' ' GRP R mRNA was up regulated . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Reverse transcription polymerase chain reaction studies , revealed the presence of mRNA for NMB and for the GRP preferring receptor ( GRP R ) . mRNA for GRP , NMB preferring receptor ( NMB R ) and bombesin receptor subtype 3 ( BRS 3 ) were not detected . [ D Phe ( 6 ) ] bombesin ( 6 13 ) methyl ester ( A 1 ) and BIM 23127 ( A 2 ) , are considered as inhibitors of binding to GRP R and NMB R , respectively . ^^^ Our data demonstrate that the proliferation induced by BLP in HT 29 cells is due to interaction with the GRP R . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Recent studies have shown that aberrantly expressed gastrin releasing peptide ( GRP ) and its receptor ( GRP R ) critically regulate tumor cell differentiation in colon cancers developing in humans and mice . ^^^ This finding suggested that the ability of GRP / GRP R to promote a well differentiated phenotype in colon cancer might reflect a re capitulation of a normal role in regulating intestinal organogenesis . ^^^ GRP / GRP R co expression in wild type mice was only observed in villous enterocytes between N 1 and N 12 . ^^^ The contribution of GRP / GRP R to villous growth was due to their act in increasing enterocyte proliferation prior to N 8 but increasing enterocyte size thereafter . ^^^ We next studied GRP / GRP R expression in human abortuses . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Prostatic neuroendocrine cells are thought to secrete these regulatory peptides and they may therefore interact with their specific , aberrantly expressed GRP receptor ( GRP R ) in prostate cancer . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
An administration of [ Leu ( 13 ) ( psi CH ( 2 ) NH ) Leu ( 14 ) ] BN ( antagonizes GRP R > neuromedin B receptor ( NMB R ) ) impaired the performance of inhibitory avoidance learning in all doses ( 16 , 32 , 64 nmol / kg ) . ^^^ While the effect was somewhat lesser than [ Leu ( 13 ) ( psi CH ( 2 ) NH ) Leu ( 14 ) ] BN , BIM 23127 ( antagonizes NMB R > GRP R ) also impaired performance in a moderate dose ( 32 nmol / kg ) . ^^^ These results showed that endogenous BN like peptides have some role ( s ) for the modulation of learning and memory , and suggest that NMB / NMB R pathway may also be involved in the memory acquisition and modulation as well as GRP / GRP R pathway . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Here we report cloning and characterization of two subtypes of BN like peptide receptors in Aves . ( 2 ) The amino acid sequence of chick GRP R ( chGRP R ) is highly identical with mammalian and amphibian GRP R , and this receptor showed high affinity for GRP , BN and synthetic bombesin agonist , [ D Phe ( 6 ) , beta Ala ( 11 ) , Phe ( 13 ) , Nle ( 14 ) ] bombesin ( 6 14 ) ( [ FAFNl ] BN ( 6 14 ) ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
METHODS : ( 1 ) RT PCR and Southern blot hybridization were performed for detection of gastrin releasing peptide GRP R mRNA ; ( 2 ) Covalent cross linking study with biotinyl bombesin and enhanced chemoluminescence ( ECL ) was performed to identify GRP R at protein levels in SGC 7901 cells . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
The mRNA for the major receptor subtype , GRP R , was present in fetal and adult sheep and rat kidneys . ^^^ In the ovine kidney , immunohistochemistry localized GRP predominantly to the thick ascending limb of the loop of Henle . mRNAs for GRP , GRP R , and BRS 3 were detected in the human embryonic kidney cell line HEK 293 , and radioimmunoassay of cell extracts and conditioned media revealed the presence of proGRP but not the amidated form . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
NE cells express a variety of peptide hormones , including the bombesin ( BBS ) like peptide , gastrin releasing peptide ( GRP ) , and its cognate receptor , GRP R . ^^^ Although there is a strong positive correlation between the degree of NE differentiation and the metastatic potential of prostate cancers , a mechanistic link between increased expression of peptide hormone receptors , such as GRP R , and proangiogenic gene expression has not been established . ^^^ In PC 3 cells , BBS stimulation of GRP R resulted in the up regulation of IL 8 and VEGF expression through a NF kappa B dependent pathway . ^^^ Together , our data demonstrate a role for BBS and GRP R in the NF kappa B dependent up regulation of proangiogenic gene expression , and suggest a possible molecular mechanism linking NE differentiation and the increased metastatic potential of androgen insensitive prostate cancers . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide ( GRP ) and its receptor ( GRP R ) are not normally expressed by epithelial cells lining the colon but are aberrantly expressed in cancer , where they act as morphogens and regulate tumor cell differentiation . ^^^ Gastrin releasing peptide ( GRP ) and its receptor ( GRP R ) are not normally expressed by epithelial cells lining the colon but are aberrantly expressed in cancer , where they act as morphogens and regulate tumor cell differentiation . ^^^ Studies of colon cancer formation in mice genetically incapable of synthesizing GRP R suggested that this receptor ' s morphogenic properties were mediated via focal adhesion kinase ( FAK ) . ^^^ We therefore set out to determine the presence of both total and phosphorylated forms of FAK in human colon cancer specimens as a function of tumor cell differentiation and GRP / GRP R co expression . ^^^ All specimens were immunohistochemically probed using antibodies recognizing GRP , GRP R , total FAK , and FAK specifically phosphorylated at tyrosine ( Y ) 397 , 407 , 576 , 577 , 861 , and 925 . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We have proposed that gastrin releasing peptide ( GRP ) and its receptor ( GRP R ) are morphogens that when aberrantly re expressed in colon cancer promote tumor cell differentiation and retard metastasis . ^^^ Under serum free conditions , GRP acting in an autocrine manner caused FAK to be phosphorylated at Y 397 ; and this could be completely inhibited either by incubating with the specific GRP R antagonist D Phe ( 6 ) ( bombesin ) methyl ester , or by upregulating FRNK using doxycycline . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
In particular , we have recently found that GRP and its cell surface receptor , GRP R , are abundantly expressed in neuroblastomas . ^^^ Moreover , more advanced stage neuroblastomas demonstrated an increased level of GRP R , suggesting an important role of GRP in aggressive tumor behavior . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
SUMMARY BACKGROUND DATA : We have previously shown that neuroblastomas secrete GRP , which binds to its cell surface receptor ( GRP R ) to stimulate cell growth in an autocrine fashion . ^^^ Human neuroblastoma cell lines ( SK N SH and SH SY5Y ) were stably transfected with the plasmid pEGFP GRP R to establish GRP R overexpression cell lines , and the effects of GRP on PTEN gene and protein expression were determined . ^^^ An increase in GRP binding capacity was confirmed in GRP R overexpressing cells , which demonstrated an accelerated constitutive cell growth rate . ^^^ PTEN gene and protein expression was significantly decreased in GRP R overexpressing cells when compared with controls . ^^^ An increase in GRP binding capacity , as a result of GRP R overexpression , down regulates PTEN expression . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
However , we have suggested that in colon cancer , GRP acts primarily as a morphogen when it and its receptor ( GRP R ) are aberrantly upregulated . ^^^ As such , GRP / GRP R act ( s ) primarily to modulate processes contributing to the assumption or maintenance of tumor differentiation . ^^^ Yet the cellular conditions affecting GRP / GRP R expression , and the biochemical pathways involved in mediating its morphogenic properties , remain to be established . ^^^ We found that confluent cells do not express GRP / GRP R . ^^^ In contrast , disaggreation and plating at subconfluent densities results in rapid GRP / GRP R upregulation followed by their progressive decrease as confluence is achieved . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Receptors for GRP and NMB ( GRP R and NMB R ) , as well as third subtype of bombesin like peptide receptor ( BRS 3 ) have been cloned . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Most leiomyomas ( 20 of 26 ) expressed GRP R but not the leiomyosarcomas . ^^^ The expression of GRP R in glands and vessels during specific phases of the cycle suggests a timely precise physiological action of GRP in these targets ; in certain uterine neoplasms , the GRP R overexpression may contribute to tumor development because GRP is a potent growth factor . ^^^ The expression of GRP R in leiomyomas may allow distinguishing them from receptor negative leiomyosarcomas ; GRP R in leiomyomas , in a subset of endometrial adenocarcinomas , carcinosarcomas , and in peritumoral vessels may be candidates for receptor targeting in vivo . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
BACKGROUND : Gastrin releasing peptide receptors ( GRPR ) are overexpressed in small cell lung carcinoma and some other human cancers . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Amplification of mRNA from a human antral cell culture preparation demonstrated the presence of two receptors of the bombesin and gastrin releasing peptide family , GRPR 1 and BRS 3 . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Epithelial cells lining the adult human colon do not normally express gastrin releasing peptide ( GRP ) or its receptor ( GRPR ) . ^^^ Because GRPR activation causes the proliferation of many cancer cell lines , GRP has been presumed to act as a clinically significant growth factor . ^^^ Yet GRP has not been shown to be expressed by colon cancers in humans nor has the effect of GRP and / or GRPR coexpression on tumor behavior been investigated . ^^^ We therefore determined GRP and GRPR expression by immunohistochemistry in 50 randomly selected colon cancers resected between 1980 and 1997 , all 37 associated lymph node and liver metastases , and 20 polyps . ^^^ Overall , 84 % of cancers aberrantly expressed GRP or GRPR , with 62 % expressing both ligand and receptor , whereas expression was not observed in adjacent normal epithelium . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide ( GRP ) regulates critical gastrointestinal functions via the GRP receptor ( GRPR ) . ^^^ Gastrin releasing peptide ( GRP ) regulates critical gastrointestinal functions via the GRP receptor ( GRPR ) . ^^^ GRPR internalization and recycling have been proposed to play an important role in the cellular response to GRP . ^^^ In comparison with epithelial cells expressing wild type GRPR , the GRPR GFP expressing cells showed similar ligand binding affinity , GRP stimulated Ca2+ signaling , and GRP initiated internalization . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
GRP receptor ( GRPR ) mRNA was also expressed in the majority of NSCLC cell lines ( nine of 14 ) . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
To investigate the molecular basis for its selectivity for the NMBR over the closely related receptor for gastrin releasing peptide ( GRPR ) , we used a chimeric receptor approach and a site directed mutagenesis approach . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
ProGRP did not compete with iodinated bombesin in binding assays on Balb 3T3 cells transfected with the known GRP receptors , GRP R or BRS 3 . ^^^ ProGRP did not compete with iodinated bombesin in binding assays on Balb 3T3 cells transfected with the known GRP receptors , GRP R or BRS 3 . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Gastrin releasing peptide receptors ( GRP R ) are upregulated in many cancers , including prostate , breast , and lung . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Bombesin ( BBN ) , a 14 amino acid peptide , is an analogue of human gastrin releasing peptide ( GRP ) that binds to GRP receptors ( GRPr ) with high affinity and specificity . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Moreover , we found that GRP receptor ( GRPR ) is expressed in GABAergic interneurons of the lateral nucleus . ^^^ These experiments provide genetic evidence that GRP and its neural circuitry operate as a negative feedback regulating fear and establish a causal relationship between Grpr gene expression , LTP , and amygdala dependent memory for fear . . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Bombesin ( BBN ) , a 14 amino acid peptide , is an analogue of human gastrin releasing peptide ( GRP ) that binds to GRP receptors ( GRPr ) with high affinity and specificity . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
BACKGROUND : Bombesin ( BBN ) , a 14 amino acid peptide , is an analogue of human gastrin releasing peptide ( GRP ) that binds to GRP receptors ( GRPr ) with high affinity and specificity . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
To test the hypothesis that such effects are mediated by known mammalian bombesin receptors [ gastrin releasing peptide ( GRP ) / bombesin preferring receptor ( GRPR ) , neuromedin B ( NMB ) receptor ( NMBR ) , and the orphan bombesin receptor subtype 3 ( BRS 3 ) ] , we analyzed the ontogeny of GRPR , NMBR , and BRS 3 gene expression in mouse lung . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
We previously reported that a gastrin releasing peptide / gastrin releasing peptide receptor ( GRP / GRPR ) autocrine growth pathway is activated early in HNSCC carcinogenesis . ^^^ In the present study , we examined the mechanism of EGFR activation by GRP / GRPR in HNSCC proliferation . ^^^ In HNSCC cells that express elevated levels of both GRPR and EGFR , we found that GRP induced rapid phosphorylation of EGFR as well as p44 / 42 MAPK activation . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Overexpression of gastrin releasing peptide ( GRP ) receptor ( GRPR ) in both androgen dependent ( AD ) and androgen independent ( AI ) human neoplastic prostate tissues provides an attractive target for prostate cancer imaging and therapy . ^^^ The goal of this study was to develop ( 64 ) Cu radiolabeled GRP analogs for PET imaging of GRPR expression in prostate cancer xenografted mice . ^^^
Interacting proteins: P30550 and P07492	Pubmed	SVM Score :0.0
Compared with wild type littermates , bombesin treated GRP receptor ( GRPR ) null mice had increased interstitial fibrosis but reduced defects in alveolarization . ^^^ All effects of GRP were abrogated in GRPR null mice . ^^^ GRPR appears to mediate all effects of GRP , but only part of the bombesin effect on alveolarization , suggesting that novel receptors may mediate some effects of bombesin in newborn lung . . ^^^