| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.89336802 |
| Evidence of interactions between syntaxin 1A and GAT 1 comes from three experimental approaches : botulinum toxin cleavage of syntaxin 1A , syntaxin 1A antisense treatments , and coimmunoprecipitation of a complex containing GAT 1 and syntaxin 1A . 0.89336802^^^ |
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| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.51438192 |
| The N terminal cytoplasmic domain of GAT 1 directly interacts with syntaxin 1A ; this interaction induces a decrease in the rate at which GABA and associated ions are transported . 0.51438192^^^ |
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| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.76314351 |
| Syntaxin 1A directly binds to the neuronal GABA transporter GAT 1 and inhibits its reuptake function . 0.76314351^^^ |
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| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.0 |
| This report examines the specifics of trafficking regulation of the rat brain GABA transporter GAT 1 by syntaxin 1A , a plasma membrane component of the SNARE complex which is involved in vesicle membrane fusion . ^^^ In hippocampal neurons , botulinum neurotoxin 1C , which specifically cleaves syntaxin 1A , down regulates plasma membrane GAT 1 levels as assessed by surface biotinylation , suggesting that syntaxin 1A is a positive regulator of GAT 1 surface expression . ^^^ These data suggest that syntaxin 1A mediates the redistribution of GAT 1 on a time scale important for the rapid regulation of extracellular GABA levels . ^^^ Expression of different syntaxin 1A constructs in Xenopus oocytes suggests that several portions of the syntaxin 1A molecule are required for the trafficking of GAT 1 . ^^^ These data suggest that the trafficking of GAT 1 will be subject to regulatory control by the many molecules known to interact with various domains of syntaxin 1A . . ^^^ |
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| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.0 |
| Syntaxin 1A inhibits GABA flux , efflux , and exchange mediated by the rat brain GABA transporter GAT 1 . ^^^ The rat brain GABA transporter GAT 1 and other members of this family are regulated by direct interactions with syntaxin 1A , a protein involved in vesicle docking and in the regulation of several ion channels and transporters . ^^^ We have shown previously that syntaxin 1A exerts its effects on GAT 1 by decreasing the net uptake of GABA and its associated ions through interactions with aspartic acid residues in the N terminal tail of GAT 1 . ^^^ To address this question , we performed GABA flux assays , measured flux and efflux associated ion currents , and assessed GABA exchange in multiple experimental systems expressing syntaxin 1A and wild type GAT 1 or GAT 1 mutants . ^^^ The syntaxin 1A mediated reduction in GABA flux and efflux was mimicked by mutations in GAT 1 at the syntaxin 1A binding site . ^^^ |
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| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.0 |
| Furthermore , syntaxin 1A , a soluble N ethylmaleimide sensitive factor attachment protein receptor ( SNARE ) protein that inhibits GAT 1 transport rates via interactions with the N terminal tail of GAT 1 was unable to regulate the GAT 1 IL4 mutant . ^^^ |
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| Interacting proteins: P30531 and Q16623 |
Pubmed |
SVM Score :0.0 |
| The function of several membrane proteins is regulated by interaction with the SNARE protein syntaxin 1A ; this includes regulation of GAT 1 , the transporter for the dominating inhibitory neurotransmitter gamma aminobutyric acid ( GABA ) . ^^^ |
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