| Interacting proteins: Q9H4B4 and P30307 |
Pubmed |
SVM Score :0.63472404 |
| Plk 3 physically interacts with Cdc25C and phosphorylates this protein phosphatase predominantly on serine 216 ( Ouyang , B . , Li , W . , Pan , H . , Meadows , J . , Hoffmann , I . , and Dai , W . ( 1999 ) Oncogene 18 , 6029 6036 ) , suggesting that the role of Plk 3 in mitosis is mediated , at least in part , through direct regulation of Cdc25C . 0.63472404^^^ |
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| Interacting proteins: Q9H4B4 and P30307 |
Pubmed |
SVM Score :0.0 |
| Cdc25C phosphorylation on serine 191 by Plk 3 promotes its nuclear translocation . ^^^ Here , we show that polo like kinase 3 ( Plk 3 ) is able to phosphorylate Cdc25C primarily on S 191 , and to a lesser extent on S 198 in vitro , both of which are within a nuclear exclusion motif . ^^^ Plk 3 WT overexpression led to a sharp increase in Cdc25C nuclear accumulation , while Plk 3 KD overexpression failed to do so . ^^^ The effect of Plk 3 overexpression on Cdc25C was reversed by coexpression of a Plk 3 siRNA . ^^^ |
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| Interacting proteins: Q9H4B4 and P30307 |
Pubmed |
SVM Score :0.0 |
| This review , therefore , will focus on the role of Plk 3 in regulating Cdc 25 phosphatase function and its effect on the cell cycle . . ^^^ |
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| Interacting proteins: Q9H4B4 and P30307 |
Pubmed |
SVM Score :0.0 |
| When Chk 2 was immunoprecipitated from Daudi cells ( Plk 3 deficient ) , it had weak kinase activity towards Cdc25C compared with Chk 2 derived from T47D cells ( Plk 3 expressing cells ) . ^^^ These data describe a functional role for Plk 3 in a pathway linking ATM , Plk 3 , Chk 2 , Cdc25C and Cdc 2 in cellular response to DNA damage . . ^^^ |
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| Interacting proteins: Q9H4B4 and P30307 |
Pubmed |
SVM Score :0.0 |
| Recombinant human Prk expressed through the baculoviral vector system is capable of phosphorylating Cdc25C , a positive regulator for the G2 / M transition . ^^^ |
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| Interacting proteins: Q9H4B4 and P30307 |
Pubmed |
SVM Score :0.0 |
| The physical association and phosphorylation of Cdc25C protein phosphatase by Prk . prk encodes a protein serine / threonine kinase involved in regulating M phase functions during the cell cycle . ^^^ We have expressed His 6 Prk and His 6 Cdc25C proteins using the baculoviral vector expression system . ^^^ Purified recombinant His 6 Prk , but not a kinase defective mutant His 6 PrkK52R , is capable of strongly phosphorylating His 6 Cdc25C in vitro . ^^^ Co immunoprecipitation and affinity column chromatography experiments demonstrate that GST Prk and native Cdc25C interact . ^^^ When co infected with His 6 Prk and His 6 Cdc25C recombinant baculoviruses , sf 9 cells produce His 6 Cdc25C antigen with an additional slower mobility band on denaturing polyacrylamide gels compared with cells infected with His 6 Cdc25C baculovirus alone . ^^^ |
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| Interacting proteins: Q9H4B4 and P30307 |
Pubmed |
SVM Score :0.0 |
| NA |
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