| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.58388125 |
| This differential phosphorylation of cdc25c was used to test whether a pharmacologic inhibitor of Plk 1 would exert the same cellular effects as interference with Plk 1 on a mRNA level . 0.58388125^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Many of these Pin 1 binding proteins are also recognized by the monoclonal antibody MPM 2 , and they include the important mitotic regulators Cdc 25 , Myt 1 , Wee 1 , Plk 1 , and Cdc 27 . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| This motif is present in known Plk 1 substrates such as Cdc 25 , and an optimal phosphopeptide containing the motif disrupted PBD substrate binding and localization of the PBD to centrosomes . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| When Plk 1 was required for cyclin B Cdc 2 activation , the action of Plk 1 was mediated primarily through suppression of Myt 1 rather than through activation of Cdc 25 . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| This review , therefore , will focus on the role of Plk 3 in regulating Cdc 25 phosphatase function and its effect on the cell cycle . . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| This analysis predicts that cancer cells manifesting a stem cell like expression profile of a death from cancer signature would exhibit the following features : a concomitantly increased expression of certain members of inhibitor of apoptosis protein ( IAP ) family ( Survivin and XIAP ) ; activation of mitotic spindle check point proteins ( BUB 1 , BUB 3 , KNTC 2 , Mad 2 , PLK 1 , PLK 4 , STK6 / Aurora A ) ; and elevated levels of certain cell cycle control / marker proteins ( CCNB 1 , CCNB 2 , CCND 1 , CCNA 2 , CDC 2 , CDC 25 , Ki 67 , USP 22 ) . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| We also noted in the chCTCF promoter several elements previously characterized in cell cycle regulated genes , including the `` cell cycle dependent element ' ' and `` cell cycle gene homology region ' ' motifs shown to be important for S / G2 specific up regulation of cdc25C , cdc 2 , cyclin A , and Plk ( polo like kinase ) gene promoters . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| The Xenopus polo like kinase 1 ( Plx 1 ) is essential during mitosis for the activation of Cdc25C , for spindle assembly , and for cyclin B degradation . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| During mitosis the Xenopus polo like kinase 1 ( Plx 1 ) plays key roles in the activation of Cdc25C , in spindle assembly , and in cyclin B degradation . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| In Xenopus , there is evidence that a kinase cascade comprised of xPlkk 1 and Plx 1 , the Xenopus polo like kinase 1 , plays a key role in the activation of Cdc25C during oocyte maturation . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Pin 1 interacts with a series of mitotic phosphoproteins , including Polo like kinase 1 , Cdc25C , and Cdc 27 , and is thought to act as a phosphorylation dependent PPIase for these target molecules . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| PLK 1 , at least in Xenopus , starts the mitotic cascade by phosphorylating and activating cdc25C phosphatase . ^^^ We wanted to understand whether the selective mitotic catastrophe in HeLa cells could be extended to other tumor types , and , if so , whether it could be attributable to a tumor specific loss of dependence on PLK 1 for cdc25C activation . ^^^ Mitotic phosphorylation of cdc25C and activation of cdk 1 was blocked by dominant negative PLK 1 in human mammary epithelial cells as well as in the tumor lines regardless of whether they underwent mitotic catastrophe . ^^^ These data strongly argue that the mitotic catastrophe is not attributable to a lack of dependence for PLK 1 in activating cdc25C . . ^^^ Dominant negative polo like kinase 1 induces mitotic catastrophe independent of cdc25C function . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| The human polo like kinase , PLK , regulates cdc2 / cyclin B through phosphorylation and activation of the cdc25C phosphatase . ^^^ Herein , we show for the first time that endogenous human PLK protein immunoprecipitated from the G2 / M arrested Jurkat cells directly phosphorylates human cdc25C . ^^^ Phosphorylation of endogenous cdc25C and recombinant cdc25C by PLK resulted in the activation of the phosphatase as assessed by dephosphorylation of cdc2 / cyclin B . ^^^ These data are the first to demonstrate that human PLK is capable of phosphorylating and positively regulating human cdc25C activity , allowing cdc25C to dephosphorylate inactive cdc2 / cyclin B . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Ubiquitination of Plk 1 by Chfr delays the activation of the Cdc25C phosphatase and the inactivation of the Wee 1 kinase , leading to a delay in Cdc 2 activation . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Plk 1 promotes nuclear translocation of human Cdc25C during prophase . ^^^ We also show that Polo like kinase 1 ( Plk 1 ) is responsible for this phosphorylation and that constitutively active Plk 1 promotes nuclear localization of Cdc25C . ^^^ These results suggest that Plk 1 phosphorylates Cdc25C on Ser 198 and regulates nuclear translocation of Cdc25C during prophase . . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Here we have identified a consensus phosphorylation sequence for Plk 1 , by testing the ability of systematically mutated peptides derived from human Cdc25C to serve as a substrate for Plk 1 . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| The mechanism involves inhibition of the enzymatic activity for polo like kinase 1 ( Plk 1 ) , rendering Cdc25C with a basal phosphatase activity that is insufficient for converting Cdc 2 to the fully active G2 / M transition kinase . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| We showed that Chfr is a ubiquitin ligase that targets polo like kinase ( Plk 1 ) for degradation , leading to delayed activation of the Cdc25C phosphatase and prolonged inhibitory phosphorylation of Cdc 2 at the G2 / M transition . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| A Polo like kinase 1 ( Plk 1 ) appears involved in an autocatalytic loop between CDC25C phosphatase and M phase promoting factor ( MPF ) in Xenopus oocytes and leads to activation of MPF that is required for germinal vesicle breakdown ( GVBD ) . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Active Plk 1 is involved in promotion of mitotic entry through activation of Cdc25C , and through nuclear import of cyclin B 1 that together activate Cdc2 / cyclin B kinase . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Crystallization and preliminary 10 ray diffraction studies on the human Plk 1 Polo box domain in complex with an unphosphorylated and a phosphorylated target peptide from Cdc25C . ^^^ |
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| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| Further examination shows that Plk 1 , p 53 and Cdc25C can form a large complex . ^^^ Plk 1 could bind to the sequence specific DNA binding domain of p 53 and active Cdc25C by hyperphosphorylation . ^^^ These results hypothesize that Plk 1 and Cdc25C participate in recovery the mitotic arrest through binding to the different domain of p 53 . ^^^ Cdc25C may first be actived by Plk 1 , and then its phosphatase activity makes p 53 dephosphorylated at Ser15 . . ^^^ |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P53350 |
Pubmed |
SVM Score :0.0 |
| NA |
|