| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| No lag phase was detected between the time when cyclin A was added and the time when H 1 histone kinase activity was produced in frog extracts , even in the presence of 2 mM vanadate , which blocks cdc 25 activity . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The results demonstrate that the treatment of asynchronous cells with 20 Gy ionizing radiation is associated with transient down regulation of the cdc 2 , cyclin A , cyclin B , and cdc 25 genes . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| This G 0 G1 arrest was accompanied by down regulation of the cell cycle regulatory genes cdc 2 , cyclin A , cyclin B , and cdc 25 . ^^^ Results obtained from mRNA half life studies demonstrate that the stability of cdc 2 , cyclin A , cyclin B , and cdc 25 transcripts is similar in control and TPA treated U 937 cells . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| They also have specific functions : cyclin A plays a role in DNA replication , whereas cyclin B are involved in the inhibition of the fusion of early endosomes and in the activation of cdc 25 phosphatase . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The role of cdc 2 , cdc 25 and cyclin A genes in the maintenance of immortalization and growth arrest in a rat embryonic fibroblast conditional cell line . ^^^ The role of the cell cycle control genes cdc 2 , cdc 25 and cyclin A in the maintenance of immortalization and in growth arrest was examined in the tsa 14 , a SV 40 T antigen rat embryonic fibroblast conditional for growth cell line . ^^^ The ability of cdc 2 , or cdc 25 , or cyclin A genes to maintain the tsa 14 immortal phenotype was also examined by electroporations of these genes into the tsa 14 cells . ^^^ Clones over expressing the electroporated cdc 2 , or cdc 25 , or cyclin A , or combinations of these genes growth arrested at the non permissive conditions similar to controls , thereby suggesting that the expression of these genes alone is insufficient for tsa 14 maintenance of immortalization . . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| A screen for dominant suppressors of the rux eye phenotype led to the identification of mutations in cyclin A , string ( cdc 25 ) , and new cell cycle genes . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| In this study , we isolated cDNAs of starfish cyclin A and Cdc 2 , and monitored extensively the cell cycle dynamics of cyclin A and cyclin B levels and their associated Cdc 2 kinase activity , Tyr phosphorylation of Cdc 2 , and Cdc 25 phosphorylation states throughout meiotic and early embryonic cleavage cycles in vivo . ^^^ Cyclin B , but not cyclin A , associated Cdc 2 was subjected to regulation via Tyr phosphorylation , and phosphorylation states of Cdc 25 correlated with cyclin B / Cdc2 kinase activity with some exceptions . ^^^ Between meiosis 1 and 2 and at the pronucleus stage , cyclin A and B levels remained low , Cdc 2 Tyr phosphorylation was undetectable , and Cdc 25 remained phosphorylated depending on MAP kinase activity , showing a good correlation between these two stages . ^^^ In the first cleavage cycle , under conditions which prevented Cdc 25 activity , cyclin A / Cdc2 was activated with a normal time course and then cyclin B / Cdc2 was activated with a significant delay , resulting in the delayed completion of M phase . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , mutations in G2 / M phase regulators cyclin A and string ( cdc 25 ) , but not cyclin B 1 , B 3 , or cdc 2 , enhanced the DmcycEJP phenotype without affecting the number of cells entering S phase , but by decreasing the number of cells entering mitosis . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| On the basis of the structure of cdk2 / CksHs1 complex and on our kinetic results , we propose that the binding of Cks proteins to C lobe of cdk 2 is stabilized by the presence of cyclin A and that it may modify the orientation of the loop carrying residues 14 and 15 and their consequent access for dephosphorylation by cdc 25 phosphatases . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| A mathematical model for the cell cycle is proposed that incorporates the known biochemical reactions involving both cyclin A and cyclin B , the interactions of these cyclins with cdc 2 and cdk 2 , and the controlling effects of cdc 25 and weel . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Cyclin A / Cdk2 complexes regulate activation of Cdk 1 and Cdc 25 phosphatases in human cells . ^^^ These results suggest that cyclin A / Cdk2 complexes are key regulators of Cdc 25 and Cdk 1 activation in human cells . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| It has only one homolog of CDKA , CDKB , CDKD , cyclin A , cyclin B , cyclin D , cyclin H , Cks , Rb , E2F , DP , DEL , Cdc 25 , and Wee 1 . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| This analysis predicts that cancer cells manifesting a stem cell like expression profile of a death from cancer signature would exhibit the following features : a concomitantly increased expression of certain members of inhibitor of apoptosis protein ( IAP ) family ( Survivin and XIAP ) ; activation of mitotic spindle check point proteins ( BUB 1 , BUB 3 , KNTC 2 , Mad 2 , PLK 1 , PLK 4 , STK6 / Aurora A ) ; and elevated levels of certain cell cycle control / marker proteins ( CCNB 1 , CCNB 2 , CCND 1 , CCNA 2 , CDC 2 , CDC 25 , Ki 67 , USP 22 ) . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Cell cycle regulation of the cyclin A , cdc25C and cdc 2 genes is based on a common mechanism of transcriptional repression . ^^^ In addition , the cdc25C , cyclin A and cdc 2 genes share an identical 5 bp region ( ' cell cycle genes homology region ' ; CHR ) starting at an identical position , six nucleotides 3 ' to the CDE . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , E2F mediated repression is dependent on a contiguous corepressor element that resembles the CHR previously established as a corepressor of the CDE in cell cycle genes derepressed in S / G2 , such as cyclin A , cdc 2 and cdc25C . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Isolation of the gene for human CENP A revealed a regulatory motif in the promoter region that directs the late S / G2 expression of other cell cycle dependent transcripts such as cdc 2 , cdc25C , and cyclin A . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Consistent with altered mitotic checkpoint control , cells expressing T displayed elevated protein levels and / or associated activities of the mitotic regulatory proteins cyclin A , cyclin B , Cdc25C and p 34 ( cdc 2 ) . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The cdc25C , cdc 2 and cyclin A promoters are controlled by transcriptional repression through two contiguous protein binding sites , termed the CDE and CHR . ^^^ While E2F mediated repression seems to be associated with genes that are up regulated early ( around mid G 1 ) , such as B myb , CDE CHR controlled genes , such as cdc25C , cdc 2 and cyclin A , become derepressed later . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The cdc25C , cyclin A and cdc 2 genes are regulated during the cell cycle through two contiguous repressor binding sites , the CDE and CHR , located in the region of transcription initiation and interacting with a factor termed CDF 1 . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The ubiquitous transcription factor , NF Y , plays a pivotal role in the cell cycle regulation of the mammalian cyclin A , cdc25C , and cdc 2 genes , in the S phase activation of the ribonucleotide reductase R 2 gene , in addition to its critical role as a key proximal promoter factor in the transcriptional regulation of the albumin , collagen , lipoprotein lipase , major histocompatibility complex class 2 , and a variety of other eukaryotic and viral genes . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Cell cycle regulation of the cyclin A gene is determined by a bipartite repressor binding site in the region of the basal promoter , termed CDE CHR , which also controls the expression of cell cycle genes upregulated in S or G 2 ( such as cdc25C ) . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The cdc25C , cyclin A and cdc 2 genes are regulated during the cell cycle through two contiguous repressor binding sites , the CDE and CHR , located in the region of transcription initiation and interacting with a factor termed CDF 1 . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| We also noted in the chCTCF promoter several elements previously characterized in cell cycle regulated genes , including the `` cell cycle dependent element ' ' and `` cell cycle gene homology region ' ' motifs shown to be important for S / G2 specific up regulation of cdc25C , cdc 2 , cyclin A , and Plk ( polo like kinase ) gene promoters . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have analysed the effect of the SV 40 large T oncoprotein ( SV LT ) on the function of a different cell cycle regulated transcriptional repressor , CDF , which is the principal regulator of the cdc25C , cyclin A and cdc 2 genes . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| In the present work we have characterized an activity present in quiescent cells and absent in cells blocked in S phase , which binds specifically to cyclin A CHR , but not to B myb , or to cdc25C , or to cdc 2 CHRs . ^^^ Both in vitro transcription and in vivo microinjection experiments demonstrate that a functional difference exists between the composite CCRE / CDE CHR repressor regions of cell cycle regulated genes such as cyclin A and cdc25C . . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Interestingly , we find that other genes , including those for CDC25C , cyclin A 2 , cyclin B 1 , CENP A , and topoisomerase IIalpha , that are normally expressed preferentially in G ( 2 ) and whose promoter regions include putative CDE and CHR elements are also downregulated in response to DNA damage . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The bipartite repressor elements , termed cell cycle dependent element ( CDE ) / cell cycle regulatory element ( CCRE ) cell cycle homology region ( CHR ) control the growth dependent transcription of the cyclin A , cdc25C , cdc 2 genes . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| We found that Mdm 2 expression rescues the temperature sensitive phenotype of tsBN 462 cells , as shown by activation of cell cycle regulated gene promoters ( B myb , cyclin A , and cdc25C ) , increased cell growth and DNA synthesis , and inhibition of apoptosis . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Alterations in p 53 , cyclin A , cyclin B 1 , and cdc25c expression were also examined in Western blots of treated HPAEC extracts . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| We had identified earlier a tandem promoter element named cell cycle dependent element ( CDE ) and cell cycle genes homology region ( CHR ) which regulates cell cycle dependent transcription of cdc25C , cyclin A and cdc 2 . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Systematic mutagenesis of the DRS enabled a consensus to be derived , and it is notable that this sequence is compatible with cell cycle gene homology region sequences associated with cell cycle dependent elements in the cyclin A , cdc 2 , and CDC25C promoters . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S phase , we investigated whether cdc25C was required for S phase transit . ^^^ Here , we show that in both nontransformed human fibroblasts and HeLa cells , cdc25C protein levels significantly increased concomitant with S phase onset and cyclin A synthesis . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Cells were arrested in G2 / M phase after 15 deoxy PGJ 2 treatment with concomitant increase in the expression of G2 / M phase regulatory protein cyclin B 1 but decrease in the expression of cdk 2 , cdk 4 , cyclin A , cyclin D 1 , cyclin E , and cdc25C . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Transient transfection assays show that YA aa inhibits transcription from the NF Y target promoters , such as cdc 2 , cyclin A , and cdc25C . ^^^ We constructed a recombinant adenovirus encoding YA aa and found that YA aa expression leads to repression of cell cycle regulatory genes , such as cyclin A , RNR R 2 , DNA polymerase alpha , cdc 2 , cyclin B , and cdc25C . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Measurements of cyclin A and B protein levels , Cdk 2 and Cdc 2 kinase activities , Cdc25C phosphorylation , and Chk 1 kinase activity were consistent with UCN 01 induced abrogation of the S / G2 phase checkpoint in ara C treated cells . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Blockade of cell cycle was associated with increased p21 / WAF1 levels and reduced amounts of cyclinB 1 , cyclinA , Cdc 2 , and Cdc25C in a p 53 independent manner . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| In both arsenical compounds treated cells , the protein levels of cyclin A and CDC25C were significantly reduced in a dose dependent manner , concomitant to the reduced activities of CDK 2 and CDC 2 associated kinase . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| This change is associated with a marked reduction in regulators of S ( cyclin A , cyclin dependent kinase ( cdk ) 2 ) , G2 / M ( cyclin B 1 , cdk 1 , cdc25c ) and G 1 ( cyclin D 1 , cyclin E , cdk 2 , cdk 4 ) phase , and an increase in p 21 and p 27 level . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NF Y transcription factor binds to CCAAT boxes on promoters of cell cycle regulatory genes such as cdc 2 , cyclin B , cdc25C , and cyclin A . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| Analysis of the cell cycle regulatory proteins demonstrated that squamocin did not change the steady state levels of Cdk 2 , Cdk 4 , cyclin A , cyclin B 1 , cyclin D 3 and cyclin E , but decreased the protein levels of Cdk 1 and Cdc25C . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| In this study , under treatment with various concentrations of PA in MDA MB 231 cell line , we checked mRNA levels for cyclin A and cyclin B 1 and the protein levels of cyclin A and cyclin B 1 , Cdc 2 ( cyclin dependent kinases ) , p 21 ( waf1 / cip1 ) and P 27 ( Kip 1 ) ( cyclin dependent kinase inhibitors ) , Cdc25C , Chk 2 and Wee 1 kinase ( cyclin dependent kinase relative factors ) in cell cycle G2 / M phase . ^^^ From those results , we determined that PA arrests MDA MB 231 cells at the G2 / M phase by ( 1 ) inhibiting synthesis or stability of mRNA and their downstream protein levels of cyclin A and cyclin B 1 , ( 2 ) increasing p 21 ( waf1 / cip1 ) and P 27 ( kip 1 ) levels , ( 3 ) increasing Chk 2 , thus causing an increase in Cdc25C phosphorylation / inactivation and inducing a decrease in Cdc 2 levels and an increase in Wee 1 level . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| This effect was associated with a reduction in the expression of regulatory proteins of G ( 2 ) M ( cyclin dependent kinase 1 and cdc25c ) and S ( cyclin A ) phases , and with an increase in the expression of apoptosis related proteins , such as p 53 and p 21 . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| However , other G2 / M regulatory molecules , such as cyclin A , Cdc 2 , Cdk 2 , Wee 1 and Cdc25C , were not modulated by curcumin treatment . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| The expression of cyclin A , cyclin B 1 , cdk 1 , and cdc25C , as well as the cdk 1 associated kinase activities , is upregulated after DNA damage , provoking a mutant p53 / NF Y dependent increase in DNA synthesis . ^^^ |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P30307 and P20248 |
Pubmed |
SVM Score :0.0 |
| NA |
|