Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.66248526
Cdc 25 was found to be phosphorylated and activated by cyclin A / Cdk2 and cyclin E / Cdk2 in vitro . 0.66248526^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.55488206
In the first cleavage cycle , under conditions which prevented Cdc 25 activity , cyclin A / Cdc2 was activated with a normal time course and then cyclin B / Cdc2 was activated with a significant delay , resulting in the delayed completion of M phase . 0.55488206^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :1.2169031
Furthermore , coimmunoprecipitation experiments revealed that PCNA interacted with Cdc25C at the G ( 2 ) / M transition , and this interaction was abolished when p 21 ( WT ) was expressed presumably due to the competition between p 21 ( WT ) and Cdc25C in the binding to PCNA . 1.2169031^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :1.1317931
Co immunoprecipitation analyses using human T cell line , Jurkat , further revealed that Cdc25C interacted with PCNA transiently when cells began to enter mitosis . 1.1317931^^^ The interaction between Cdc25C and PCNA was confirmed in vitro and in vivo . 0.78183246^^^ Cdc25C interacts with PCNA at G2 / M transition . 0.67073354^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Entry into mitosis depends upon activation of the dual specificity phosphatase Cdc25C , which dephosphorylates and activates the cyclin B Cdc 2 complex . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We have overexpressed and purified human dual specificity phosphatase cdc25C from a prokaryotic expression system at high levels and in a soluble , active form , and have studied and quantified its potential to interact with cdks , cyclins and preformed cdk cyclin complexes by fluorescence spectroscopy and size exclusion chromatography . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Oscillation of MPF is accompanied by periodic association between cdc 25 and cdc 2 cyclin B . ^^^ These results suggest that the mechanism by which cdc 25 triggers cdc 2 activation involves a periodic physical association between cdc 25 and the cyclin B cdc 2 complex and also that mitotic control can be affected by mechanisms other than transcriptional regulation of the cdc 25 gene . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Screening for antimitotic compounds using the cdc 25 tyrosine phosphatase , an activator of the mitosis inducing p34cdc2 / cyclin Bcdc 13 protein kinase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
No lag phase was detected between the time when cyclin A was added and the time when H 1 histone kinase activity was produced in frog extracts , even in the presence of 2 mM vanadate , which blocks cdc 25 activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Furthermore , the addition of okadaic acid to egg extracts arrested in S phase by aphidicolin causes phosphorylation and activation of the cdc 25 protein before cyclin B / cdc2 kinase activation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The results demonstrate that the treatment of asynchronous cells with 20 Gy ionizing radiation is associated with transient down regulation of the cdc 2 , cyclin A , cyclin B , and cdc 25 genes . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
A family of proteins homologous to the cdc 25 gene product of the fission yeast bear specific protein tyrosine phosphatase activity involved in the activation of the p34cdc2 cyclin B kinase . ^^^ Cell Biol . 115 : 1 17 ) , the data presented here suggest that separation in two distinct cellular compartments of the cdc 25 phosphatase and its substrate p34cdc2 cyclin B may be of importance in the regulation of the cdc 2 kinase activity . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This G 0 G1 arrest was accompanied by down regulation of the cell cycle regulatory genes cdc 2 , cyclin A , cyclin B , and cdc 25 . ^^^ Results obtained from mRNA half life studies demonstrate that the stability of cdc 2 , cyclin A , cyclin B , and cdc 25 transcripts is similar in control and TPA treated U 937 cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We have now identified two other gene products required for mitosis in A . nidulans . nimT encodes a protein similar to the fission yeast cdc 25 tyrosine phosphatase and is required for the conversion of pre MPF to MPF and nimE encodes a B type cyclin which is a subunit of MPF . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The motif may represent an activating domain that has to be provided to cdc 25 by intermolecular interaction with cyclin B . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The periodic activation of cdc 2 kinase , which is required for entry into M phase , is regulated by subunit association with cyclin B , the cdc 25 , wee 1 , mik 1 gene products and differential phosphorylation of the cdc 2 protein . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
To elucidate the mechanism for induction of mitosis , phosphorylation of cdc 25 has been investigated using recombinant proteins . cdc 25 is phosphorylated by both cyclin A / p34cdc2 and cyclin B / p34cdc2 at similar sets of multiple sites in vitro . ^^^ This phosphorylation retards its electrophoretical mobility and activates its ability to increase cyclin B / p34cdc2 kinase activity three to fourfold in vitro , as found for endogenous Xenopus cdc 25 in M phase extracts . ^^^ At physiological concentrations these mutants can not activate cyclin B / p34cdc2 in cdc 25 immunodepleted oocyte extracts , suggesting that a positive feed back loop between cdc 2 and cdc 25 is necessary for the full activation of cyclin B / p34cdc2 that induces abrupt entry into mitosis in vivo . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The endogenous store of preMPF can not be recruited by cdc 25 protein phosphatase or cyclin protein microinjections . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We measured the mRNA levels of beta actin , transferrin receptor , and two cell cycle related genes , cyclin B and cdc 25 , in early mouse embryos by the RT PCR . ^^^ These results suggest that the rate of mRNA degradation is different between cyclin B and cdc 25 during early embryogenesis . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
During metaphase 1 , the reactivation of MPF activity can be disrupted in two different ways : 1 ) cycloheximide , an inhibitor of protein synthesis , by preventing the synthesis of new cyclins , provokes the disappearance of MPF kinase activity and the reformation of a nucleus ; 2 ) when the cAMP level is increased during the metaphase 1 period in cdc 25 injected oocytes , MPF kinase activity drops following a rephosphorylation of tyrosine 15 of p34cdc2 , while the cyclin turn over remains unaffected . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This situation is obviously different from that in immature Xenopus oocytes , in which the cdc 2 cyclin B complex preexists with cdc 2 phosphorylated on both Tyr 15 and Thr 161 , thereby requiring Tyr 15 dephosphorylation catalyzed by cdc 25 phosphatase for MPF activation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
To contribute to a deeper understanding of M phase control in eukaryotic cells , we have constructed a model based on the biochemistry of M phase promoting factor ( MPF ) in Xenopus oocyte extracts , where there is evidence for two positive feedback loops ( MPF stimulates its own production by activating Cdc 25 and inhibiting Wee 1 ) and a negative feedback loop ( MPF stimulates its own destruction by indirectly activating the ubiquitin pathway that degrades its cyclin subunit ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
They also have specific functions : cyclin A plays a role in DNA replication , whereas cyclin B are involved in the inhibition of the fusion of early endosomes and in the activation of cdc 25 phosphatase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Functional analysis of the P box , a domain in cyclin B required for the activation of Cdc 25 . ^^^ Cyclin B cdc 2 complexes are kept inactive by inhibitory phosphorylations on Thr 14 and Tyr 15 of cdc 2 until they are dephosphorylated at the end of G 2 by the phosphatase cdc 25 . ^^^ Recent work has suggested that a small region of cyclin B , which we call the P box , may contribute part of a phosphatase activating domain to cdc 25 . ^^^ Individual conservative substitutions at three invariant residues within the P box yield mutant cyclin B proteins that bind cdc 2 in vitro and then show the predicted cell cycle arrest , with cdc 25 remaining in the low activity interphase form and cyclin B cdc 2 complexes remaining phosphorylated and inactive . ^^^ While the low activity interphase form of cdc 25 can not act on cdc 2 complexed with a mutant P box cyclin , the high activity M phase form of cdc 25 can . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Phosphorylation and activation of human cdc 25 C by cdc 2 cyclin B and its involvement in the self amplification of MPF at mitosis . ^^^ It has been found previously that cdc 25 is the tyrosine phosphatase responsible for dephosphorylating and activating cdc 2 cyclin B . ^^^ Phosphorylation of cdc 25 C in mitotic HeLa extracts or by cdc 2 cyclin B increases its catalytic activity . cdc 25 C is not a substrate of the cyclin A associated kinases . cdc 25 C is able to activate cdc 2 cyclin B 1 in Xenopus egg extracts and to induce Xenopus oocyte maturation , but only after stable thiophosphorylation . ^^^ This demonstrates that phosphorylation of cdc 25 C is required for the activation of cdc 2 cyclin B and entry into M phase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Expression of cyclin B , cdc 25 , wee 1 and cdc 2 was studied during cell cycle arrest by Northern blot hybridization . ^^^ Expression of cyclin B , cdc 25 and cdc 2 fluctuated in association with cell cycle progression towards the G2 / M phase , while wee 1 expression remained constant in untreated cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In vitro , CDC2 / cyclin B 1 is activated by treatment with the members of the Cdc 25 family of phosphatases . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cyclin B , Cdc 25 , and Cyclin E each act as limiting regulators in Drosophila in specific cell types at particular developmental stages . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We also identified Arg 452 and Ser 449 as two crucial residues that could be directly involved in the molecular interactions between Cdc 25 and cyclin Cdk proteins . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The role of cdc 2 , cdc 25 and cyclin A genes in the maintenance of immortalization and growth arrest in a rat embryonic fibroblast conditional cell line . ^^^ The role of the cell cycle control genes cdc 2 , cdc 25 and cyclin A in the maintenance of immortalization and in growth arrest was examined in the tsa 14 , a SV 40 T antigen rat embryonic fibroblast conditional for growth cell line . ^^^ The ability of cdc 2 , or cdc 25 , or cyclin A genes to maintain the tsa 14 immortal phenotype was also examined by electroporations of these genes into the tsa 14 cells . ^^^ Clones over expressing the electroporated cdc 2 , or cdc 25 , or cyclin A , or combinations of these genes growth arrested at the non permissive conditions similar to controls , thereby suggesting that the expression of these genes alone is insufficient for tsa 14 maintenance of immortalization . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
These results raise the possibility that the intermediate form T YP may be involved in the autocatalytic amplification of the p 34 ( cdc 2 ) / cyclin B complex through phosphorylation / activation of the cdc 25 phosphatase and phosphorylation / inactivation of the wee 1 kinase . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Immunodetection of other proteins that may be initiator proteins ( p 34 , cdc 25 , and microtubule associated protein kinases ) in immature oocytes , and the absence of cyclin in these oocytes , suggest an important role for cyclin B . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
A screen for dominant suppressors of the rux eye phenotype led to the identification of mutations in cyclin A , string ( cdc 25 ) , and new cell cycle genes . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
When tsBN 2 cells arrested in S phase were incubated at 40 degrees C in the presence of cycloheximide , Cdc25B , but not Cdc25A and C , among a family of dual specificity phosphatases , Cdc 25 , was lost coincidentally with the lack of the activation of p 34 ( cdc 2 ) / cyclin B . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Hyperphosphorylation of the N terminal domain of Cdc 25 regulates activity toward cyclin B1 / Cdc2 but not cyclin A / Cdk2 . ^^^ Cdc 25 regulates entry into mitosis by regulating the activation of cyclin B / cdc2 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Early MAP kinase activation did not interfere significantly with DNA replication , cyclin synthesis , or association of cyclins with Cdc 2 , but it did prevent hyperphosphorylation of Cdc 25 and Wee 1 and activation of Cdc2 / cyclin complexes . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Production of a soluble cyclin B / cdc2 substrate for cdc 25 phosphatase . ^^^ The assay is based on the cdc 25 specific dephosphorylation and activation of the phosphorylated cyclin B1 / cdc2 substrate ( PY 15 ) , using the increased histone H 1 kinase activity of the activated PY 15 as a read out of cdc 25 activity . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cut 12 is localized to the SPB throughout the cell cycle , predominantly around the inner face of the interphase SPB , adjacent to the nucleus . cut 12 ( + ) is allelic to stf 1 ( + ) ; stf1 . 1 is a gain of function mutation bypassing the requirement for the Cdc 25 tyrosine phosphatase , which normally dephosphorylates and activates the p 34 ( cdc 2 ) / cyclin B kinase to promote the onset of mitosis . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Activation of the cyclin B cdc 2 kinase mitotic inducer involves dephosphorylation of two inhibitory residues , tyrosine 15 and threonine 14 , cdc 25 is the specific phosphatase that directly dephosphorylates and activates the cdc 2 kinase , cdc 25 activity is regulated by phosphorylation . ^^^ Although the cyclin B cdc 2 complex plays a role in activating cdc 25 , it is highly probable that a distinct protein kinase is involved as a trigger in cdc 25 activation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Constitutive overproduction of the transcriptional regulator dE2F increased expression of the S and M phase initiators Cyclin E and String ( Cdc 25 ) , thereby accelerating cell proliferation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In the present paper , the activity of Cdk 2 cyclin E is incorporated into an existing comprehensive model of the cell cycle engine as an activity operating in parallel with the mitosis promotion factor ( MPF ) on the phosphatase Cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Surprisingly , mutations in G2 / M phase regulators cyclin A and string ( cdc 25 ) , but not cyclin B 1 , B 3 , or cdc 2 , enhanced the DmcycEJP phenotype without affecting the number of cells entering S phase , but by decreasing the number of cells entering mitosis . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
On the basis of the structure of cdk2 / CksHs1 complex and on our kinetic results , we propose that the binding of Cks proteins to C lobe of cdk 2 is stabilized by the presence of cyclin A and that it may modify the orientation of the loop carrying residues 14 and 15 and their consequent access for dephosphorylation by cdc 25 phosphatases . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cyclin B phosphorylation is not required for Cdc 2 kinase activity ; both the prophase complex dephosphorylated on Cdc 2 with Cdc 25 and the metaphase complex dephosphorylated on cyclin B with protein phosphatase 2A display high kinase activities . ^^^ We demonstrate that Cdc 2 itself is the cyclin B kinase ; cyclin B phosphorylation requires Cdc 2 activity both in vivo ( sensitivity to vitamin K 3 , a Cdc 25 inhibitor ) and in vitro ( copurification with Cdc 2 cyclin B , requirement of Cdc 2 dephosphorylation , and sensitivity to chemical inhibitors of cyclin dependent kinases ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Both point and deletion mutations show synthetic lethality at room temperature with temperature sensitive mutations in cyclin B ( cdc 13 117 ) or the phosphatase cdc 25 ( cdc 25 22 ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Reciprocal shift experiments with cdc 25 and cdc 28 mutants showed that the major cell cycle arrest point was located in the Start area , at or near the CDC 25 mediated step , before the step mediated by the CDC 28 cyclin dependent kinase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Further analysis of constituent cell cycle proteins of these kinases by Western blot showed that A 23187 alone could not induce changes in cdc 2 , cdc 25 , or ERK 2 but induced reduction of cyclin B 1 . ^^^ Moreover , accumulation of both cdc 25 and cyclin B 1 was detected at 15 h . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Human Cdc 25 phosphatases play important roles in cell cycle regulation by removing inhibitory phosphates from tyrosine and threonine residues of cyclin dependent kinases . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
A mathematical model for the cell cycle is proposed that incorporates the known biochemical reactions involving both cyclin A and cyclin B , the interactions of these cyclins with cdc 2 and cdk 2 , and the controlling effects of cdc 25 and weel . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In other systems , Chk 1 kinase phosphorylates and suppresses the activity of Cdc 25 phosphatase : the resulting failure to remove inhibitory phosphate from cyclin dependent kinase 1 ( Cdk 1 ) prevents entry into mitosis [ 4 ] [ 5 ] . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The detailed model could be used to explain various experiments relevant to G2DDC reported recently , including the nuclear export of 14 3 3 bound Cdc 25 , the down regulation of cyclin B 1 expression by p 53 , the effect of Chk 1 and p 53 on Cdc 25 levels , and Wee 1 degradation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Other potentially useful cell cycle areas for exploration include cyclin CDK interactions , Cdc 25 activation of cyclin CDK complexes , ubiquitin mediated proteolysis of cyclins , cell cycle check point kinases like Chk 1 , and recently identified oncogenic cell cycle related aurora and polo like kinases . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In mammalian cells , three dual specificity phosphatases called CDC 25 activate CDKs / cyclin complexes . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We have dissected the contribution of these structures to MPF activation in fertilized eggs and in egg fragments containing different combinations of nuclei , centrosomes , and microtubules by following the behavior of Cdc 2 ( the kinase component of MPF ) , the regulatory subunit cyclin B , and the activating phosphatase Cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
After in vitro activation of lymphocytes from healthy individuals , cyclin B 1 and cdc 25 expression and ubiquitination , p 34 cdc2 activity , NORs morphology , and C23 / nucleolin localization showed a 72 to 96 hour cyclic pattern that led to a biologic state similar to baseline . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Immunoblot analysis of embryos whose cell divisions are synchronized by inducible String ( cdc 25 homolog ) demonstrated that cyclin B was degraded during low O 2 conditions in interphase arrested embryos but not in those arrested in metaphase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cdc 25 dependent activation of cyclin A / cdk2 is blocked in G 2 phase arrested cells independently of ATM / ATR . ^^^ We show that cyclin A / cdk2 is activated in early G 2 phase by a cdc 25 activity . ^^^ In the G 2 phase checkpoint arrest initiated in response to various forms of DNA damage , the cdc 25 dependent activation of both cyclin A / cdk2 and cyclin B1 / cdc2 is blocked . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In the G 2 phase cell cycle checkpoint arrest , the cdc 25 dependent activation of cyclin B / cdc2 , a critical step in regulating entry into mitosis , is blocked . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
M phase promoting factor is a complex of cdc 2 and cyclin B that is regulated positively by cdc 25 phosphatase and negatively by wee 1 kinase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
To directly test the morphogenic function of cell division , we have utilised a microinduction technique to locally and transiently manipulate the expression in transgenic plants of two genes encoding putative effectors of the cell cycle , a tobacco A type cyclin and a yeast cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Nuclear export of Survivin is independent of the export of other shuttling proteins that control the G2 / M phase transition , such as cyclin B 1 and cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Our results suggest that the PDP induced G2 / M arrest in HT 29 cells was through the intracellular events including ( a ) inhibition of normal mitotic spindle formation , ( b ) elevation of cyclin B1 / cdc2 kinase activity , ( c ) concomitant increases in cdc 25 A phosphatase and cdk 7 kinase activity , and ( d ) down regulation of the wee 1 protein expression . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The Vpr protein of primate lentiviruses arrests cell cycling at the G ( 2 ) / M phase through an inactivation of cyclin B p 34 ( cdc 2 ) and its upstream regulator cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
For this we studied the effect of CA4P on selected markers of apoptosis [ caspases 9 and 3 , poly ( ADP ribose ) polymerase ( PARP ) , bcl 2 , and bax ] and G 2 M protein regulators ( p 53 , MDM 2 , 14 3 3sigma , GADD 45 , cdc 2 , cdc 25 , chk 1 , wee 1 , p 21 , and cyclin B 1 ) . ^^^ Exposure of WSU CLL cells to 4 and 5 nM CA4P was associated with overproduction of total p 53 and no dramatic change in MDM 2 , 14 3 3sigma , GADD 45 , the cyclin dependent kinase cdc 2 , its inhibitory phosphorylation , the cdc 2 inhibitory kinase ( wee 1 ) , chk 1 , or cdc 25 hyperphosphorylation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Using BrdU labeling as an index of cell proliferation , we found that the in vitro neuroproliferative effect of VEGF was associated with up regulation of E2F family transcription factors , cyclin D 1 , cyclin E , and cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cdc 2 regulation also involves positive feedback , with active Cdc 2 cyclin B stimulating its activator Cdc 25 ( refs 5 7 ) and inactivating its inhibitors Wee 1 and Myt 1 ( refs 8 11 ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
These results suggest that Drosophila Cdc 25 may directly or indirectly increase the kinase activity of Cyclin E Cdk 2 complexes in vivo , thus driving arrested neuroblasts into cell division . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Activation of Cdc2 / cyclin B kinase and entry into mitosis requires dephosphorylation of inhibitory sites on Cdc 2 by Cdc 25 phosphatase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
When Plk 1 was required for cyclin B Cdc 2 activation , the action of Plk 1 was mediated primarily through suppression of Myt 1 rather than through activation of Cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Active Cdc 25 phosphatase released Cdc 2 cyclin B from the detergent insoluble fraction independently of its phosphatase activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Interestingly , biochemical fractionation studies revealed that the active form of Cdk 1 , a fraction of total cyclin B 1 , and the Cdc 25 phosphatases reside predominantly in the cytoplasm of infected cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cyclin A / Cdk2 complexes regulate activation of Cdk 1 and Cdc 25 phosphatases in human cells . ^^^ In Xenopus oocytes and early embryos , accumulation of cyclin B activates Cdk 1 , which then phosphorylates and activates the positive regulator Cdc 25 in an autocatalytic feedback loop . ^^^ These results suggest that cyclin A / Cdk2 complexes are key regulators of Cdc 25 and Cdk 1 activation in human cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Stage 4 oocytes contain a store of inactive pre MPF ( Tyr 15 phosphorylated Cdc 2 bound to cyclin B 2 ) ; the Cdc 25 phosphatase that catalyzes Tyr 15 dephosphorylation of Cdc 2 is also present . ^^^ We report that when cyclin B is overexpressed in stage 4 oocytes , MPF autoamplification does not occur and the newly formed cyclin B Cdc 2 complexes are inactivated by Tyr 15 phosphorylation , indicating that Myt 1 kinase remains active and that Cdc 25 is prevented to be activated . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cdc 2 cyclin B induced G 2 to M transition in perch oocyte is dependent on Cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This nuclear retention , which strictly depends on association with endogenous p 21 , prevents activation of cyclin B 1 Cdk1 by Cdc 25 and Cdk activating kinase as well as its recruitment to the centrosome . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The traditional role of the Cdc 25 family of dual specificity phosphatases is to activate cyclin dependent kinases ( CDKs ) to enable progression through the cell cycle . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In post blastoderm Drosophila embryos , zygotic expression of the stg ( cdc 25 ) phosphatase in G 2 activates cyclin / cdc2 kinases and triggers mitosis . ^^^ After the introduction of G 1 S control midway through embryogenesis , histone expression depends on DNA replication and the function of cyclin E , and no longer requires stg ( cdc 25 ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We speculate that caffeine may enhance MOLT 4 cell entrance into the S phase through activation of Cdc 25 , which in turn activates cyclin dependent protein kinases ( CDKs ) including CDK 2 and drives the cell cycle progression ; while degradation of cyclin E by the ubiquitin / proteasome pathway may account for the decreased levels of cyclin E in these cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
It has only one homolog of CDKA , CDKB , CDKD , cyclin A , cyclin B , cyclin D , cyclin H , Cks , Rb , E2F , DP , DEL , Cdc 25 , and Wee 1 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Promotion of neuronal cells proliferation by VEGF is also associated with the increased expression of VEGFR 2 receptors and up regulation of E2F family transcription factors , cyclin D 1 , cyclin E , and cdc 25 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Schizosaccharomyces pombe Flp 1 phosphatase down regulates CDK / cyclin activity , controlling the degradation of the Cdc 25 tyrosine phosphatase for fission yeast cells to undergo cytokinesis . ^^^ Our results support a Cdc 14 conserved inhibitory mechanism acting on S . pombe Cdc 25 protein and suggest that human cells may regulate Cdc 25 in a similar manner to inactivate Cdk 1 mitotic cyclin complexes . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This effect was strongly associated with a concomitant decrease in the level of cyclin B 1 , Cdc 25 and Cdc 2 , and increase in phospho Cdc 2 ( Tyr 15 ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cdk 2 Speedy / Ringo A 2 also phosphorylated authentic Cdk 2 substrates , such as Cdc 25 proteins , which contain non canonical CDK phosphorylation sites , nearly as well as Cdk 2 cyclin A . ^^^ Phosphopeptide mapping indicated that Cdk 2 Speedy / Ringo A 2 and Cdk 2 cyclin A phosphorylate distinct subsets of sites on Cdc 25 proteins . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cdc 25 phosphatases are essential for the activation of mitotic cyclin Cdks , but the precise roles of the three mammalian isoforms ( A , B , and C ) are unclear . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Low cyclin B associated kinase activity , as well as the status of p34cdc2 and cdc25C phosphorylation , indicates that the cascade of reactions which drives the cell into mitosis has not been initiated . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cell cycle regulation of the cyclin A , cdc25C and cdc 2 genes is based on a common mechanism of transcriptional repression . ^^^ In addition , the cdc25C , cyclin A and cdc 2 genes share an identical 5 bp region ( ' cell cycle genes homology region ' ; CHR ) starting at an identical position , six nucleotides 3 ' to the CDE . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We account for this anomaly by our observation that the cdc 2 activator , cdc25C , is a cytoplasmic protein that , like cyclin B 1 , enters the nucleus at the G2 / M transition . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cyclin A 1 , B 1 , B 2 , and E 1 levels , Cdc 2 and Cdk 2 kinase activity , and Cdc25C phosphorylation states were monitored during early Xenopus embryonic cell cycles . ^^^ Cyclin B1 / Cdc2 kinase activity oscillated , peaking before each cleavage , and Cdc25C alternated between a highly phosphorylated and a less phosphorylated form that correlated with high and low cyclin B1 / Cdc2 kinase activity , respectively . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Surprisingly , E2F mediated repression is dependent on a contiguous corepressor element that resembles the CHR previously established as a corepressor of the CDE in cell cycle genes derepressed in S / G2 , such as cyclin A , cdc 2 and cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Regulation of the acquisition of meiotic competence in the mouse : changes in the subcellular localization of cdc 2 , cyclin B 1 , cdc25C and wee 1 , and in the concentration of these proteins and their transcripts . ^^^ Results of immunoblotting experiments demonstrate that associated with the acquisition of meiotic competence is an increase in the concentration of cdc25C , an activator of p34cdc2 / cyclin B kinase , and a decrease in wee 1 , an inhibitor of cdc2 / cyclin B kinase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Isolation of the gene for human CENP A revealed a regulatory motif in the promoter region that directs the late S / G2 expression of other cell cycle dependent transcripts such as cdc 2 , cdc25C , and cyclin A . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Consistent with altered mitotic checkpoint control , cells expressing T displayed elevated protein levels and / or associated activities of the mitotic regulatory proteins cyclin A , cyclin B , Cdc25C and p 34 ( cdc 2 ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Thus the block in cyclin B / cdc2 activation is in part due to the physical separation of cyclin B / cdc2 , localised in the cytoplasm , from the cdc25B and cdc25C phosphatases localised in the nucleus . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The cdc25C , cdc 2 and cyclin A promoters are controlled by transcriptional repression through two contiguous protein binding sites , termed the CDE and CHR . ^^^ While E2F mediated repression seems to be associated with genes that are up regulated early ( around mid G 1 ) , such as B myb , CDE CHR controlled genes , such as cdc25C , cdc 2 and cyclin A , become derepressed later . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The cdc25C , cyclin A and cdc 2 genes are regulated during the cell cycle through two contiguous repressor binding sites , the CDE and CHR , located in the region of transcription initiation and interacting with a factor termed CDF 1 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The ubiquitous transcription factor , NF Y , plays a pivotal role in the cell cycle regulation of the mammalian cyclin A , cdc25C , and cdc 2 genes , in the S phase activation of the ribonucleotide reductase R 2 gene , in addition to its critical role as a key proximal promoter factor in the transcriptional regulation of the albumin , collagen , lipoprotein lipase , major histocompatibility complex class 2 , and a variety of other eukaryotic and viral genes . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Activation of cyclin B / Cdk1 at the onset of mitosis is accomplished by a phosphatase , Cdc25C , that interacts with cyclin B / Cdk1 in an autocatalytic feedback loop to remove the inhibitory phosphate at Tyr 15 and activate kinase activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In contrast , activation of cyclin A cdc 2 kinase does not require Plx 1 activity , and cyclin A cdc 2 kinase fails to activate Plx 1 and its consequence , cdc25c activation in cycling extracts . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cell cycle regulation of the cyclin A gene is determined by a bipartite repressor binding site in the region of the basal promoter , termed CDE CHR , which also controls the expression of cell cycle genes upregulated in S or G 2 ( such as cdc25C ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The cdc25C , cyclin A and cdc 2 genes are regulated during the cell cycle through two contiguous repressor binding sites , the CDE and CHR , located in the region of transcription initiation and interacting with a factor termed CDF 1 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We also noted in the chCTCF promoter several elements previously characterized in cell cycle regulated genes , including the `` cell cycle dependent element ' ' and `` cell cycle gene homology region ' ' motifs shown to be important for S / G2 specific up regulation of cdc25C , cdc 2 , cyclin A , and Plk ( polo like kinase ) gene promoters . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The Xenopus polo like kinase 1 ( Plx 1 ) is essential during mitosis for the activation of Cdc25C , for spindle assembly , and for cyclin B degradation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
METHODS : Expression of cyclin B 1 , cdc 2 , cdc25c , total protein and DNA content were examined by flow cytometry in two lung cancer cell lines . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Increased expression of cyclin B 1 and Cdc25C inhibited the Gadd 45 mediated G2 / M arrest in human fibroblasts , indicating that the mechanism of Gadd 45 mediated G2 / M checkpoint is at least in part through modulation of the activity of the G 2 specific kinase , cyclin B1 / p34 ( cdc 2 ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In the present study , we have analysed the effect of the SV 40 large T oncoprotein ( SV LT ) on the function of a different cell cycle regulated transcriptional repressor , CDF , which is the principal regulator of the cdc25C , cyclin A and cdc 2 genes . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cytoplasmic localization of human cdc25C during interphase requires an intact 14 3 3 binding site . cdc25C induces mitosis by activating the cdc 2 cyclin B complex . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Overexpressing Cdc25C together with cyclin B 1 does shorten the G 2 phase and can override the unreplicated DNA checkpoint , but much less efficiently than overexpressing Cdc25B . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We also demonstrate that although the 1 , 25D ( 3 ) treated cells exhibit elevated levels of cyclin B 1 , Cdc25C , and Cdk 7 , which are positive regulators of the G ( 2 ) / M traverse , these cells have decreased protein levels of p 34 ( cdc 2 ) and decreased p 34 ( cdc 2 ) kinase activity . ^^^ The data also suggest that the ability of some cells to traverse this block may be the result of cellular compensatory mechanisms responding to decreased p 34 ( cdc 2 ) activity by increasing the levels of other regulators of the G ( 2 ) traverse , such as cyclin B 1 , Cdc25C , and Cdk7 . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
During mitosis the Xenopus polo like kinase 1 ( Plx 1 ) plays key roles in the activation of Cdc25C , in spindle assembly , and in cyclin B degradation . ^^^ Microinjection of mRNA encoding S128D / T201D Plx 1 into Xenopus oocytes induced directly the activation of both Cdc25C and cyclin B Cdc 2 . ^^^ These results demonstrate that in Xenopus laevis activation of Plx 1 is sufficient for the activation of Cdc25C at the initiation of mitosis and that inactivation of Plx 1 is required for complete degradation of cyclin B 2 after anaphase and completion of cytokinesis . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In the present work we have characterized an activity present in quiescent cells and absent in cells blocked in S phase , which binds specifically to cyclin A CHR , but not to B myb , or to cdc25C , or to cdc 2 CHRs . ^^^ Both in vitro transcription and in vivo microinjection experiments demonstrate that a functional difference exists between the composite CCRE / CDE CHR repressor regions of cell cycle regulated genes such as cyclin A and cdc25C . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Interestingly , we find that other genes , including those for CDC25C , cyclin A 2 , cyclin B 1 , CENP A , and topoisomerase IIalpha , that are normally expressed preferentially in G ( 2 ) and whose promoter regions include putative CDE and CHR elements are also downregulated in response to DNA damage . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The bipartite repressor elements , termed cell cycle dependent element ( CDE ) / cell cycle regulatory element ( CCRE ) cell cycle homology region ( CHR ) control the growth dependent transcription of the cyclin A , cdc25C , cdc 2 genes . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We found that Mdm 2 expression rescues the temperature sensitive phenotype of tsBN 462 cells , as shown by activation of cell cycle regulated gene promoters ( B myb , cyclin A , and cdc25C ) , increased cell growth and DNA synthesis , and inhibition of apoptosis . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Alterations in p 53 , cyclin A , cyclin B 1 , and cdc25c expression were also examined in Western blots of treated HPAEC extracts . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The highest levels of Cdc25A and Cdc25C expression and their subcellular localization during meiotic prophase coincide with that of cyclin A 1 , and when overexpressed in HeLa cells , cyclin A 1 coimmunoprecipitates with Cdc25A . ^^^ Furthermore , the protein kinase complexes consisting of cyclin A 1 and either Cdc 2 or Cdk 2 phosphorylate both Cdc25A and Cdc25C in vitro . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Chk 1 phosphorylates cdc25C on serine 216 and inactivates it whereas cdc25C dephosphorylates tyrosine 15 phosphate of cdc 2 and activates the cdc 2 cyclin B complex . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We had identified earlier a tandem promoter element named cell cycle dependent element ( CDE ) and cell cycle genes homology region ( CHR ) which regulates cell cycle dependent transcription of cdc25C , cyclin A and cdc 2 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Acquisition of meiotic competence in mouse oocytes : absolute amounts of p 34 ( cdc 2 ) , cyclin B 1 , cdc25C , and wee 1 in meiotically incompetent and competent oocytes . ^^^ We report measurements of the absolute mass of p 34 ( cdc 2 ) and cyclin B 1 , as well as the two proximal regulators of MPF , namely cdc25C and wee 1 , in meiotically incompetent and competent mouse oocytes . ^^^ We find that the numbers of molecules of p 34 ( cdc 2 ) , cyclin B 1 , cdc25C , and wee 1 in meiotically incompetent oocytes are 1 . 4 10 10 ( 6 ) , 11 . 3 10 10 ( 6 ) , 24 . 6 10 10 ( 6 ) , 15 . 6 10 10 ( 6 ) , respectively , and in meiotically competent oocytes the numbers are 14 . 3 10 10 ( 6 ) , 95 . 5 10 10 ( 6 ) , 80 . 0 10 10 ( 6 ) , 40 . 1 10 10 ( 6 ) , respectively . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NF Y mediates the transcriptional inhibition of the cyclin B 1 , cyclin B 2 , and cdc25C promoters upon induced G 2 arrest . ^^^ During normal cell cycles , the function of mitotic cyclin cdk 1 complexes , as well as of cdc25C phosphatase , is required for G 2 phase progression . ^^^ In our experimental conditions , the integrity of the CCAAT boxes of cyclin B 1 , cyclin B 2 , and cdc25C promoters , as well as the presence of a functional NF Y complex , is strictly required for the transcriptional inhibition of these promoters . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The cyclin N terminal helix mutations have a significant effect on the interaction between the cyclin CDK complex and specific substrates , Xenopus Cdc 6 and Cdc25C . ^^^ These results suggest that the N terminal helix of mitotic cyclins is required for specific interactions with CDKs and that to interact with CDK , specific substrates Cdc 6 and Cdc25C require the CDK to be associated with a cyclin . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The cdc25C phosphatase dephosphorylates cdc 2 kinase which then in complex with cyclin B can catalyse transition from the G ( 2 ) phase to mitosis . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The polo like kinase Plx 1 is required for activation of the phosphatase Cdc25C and cyclin B Cdc 2 in Xenopus oocytes . ^^^ PKI stimulated the synthesis of Mos and activation of both the MAPK pathway and the Plx1 / Cdc25C / cyclin B Cdc 2 pathway . ^^^ Immunodepletion of Plx 1 completely inhibited activation of Cdc25C and cyclin B Cdc 2 by PKI , indicating that Plx 1 is necessary for Cdc25C activation . ^^^ In extracts containing fully activated Plx 1 and Cdc25C , inhibition of cyclin B Cdc 2 by p 21 ( Cip 1 ) had no significant effect on either the phosphorylation of Cdc25C or the activity of Plx 1 . ^^^ These results demonstrate that maintenance of Plx 1 and Cdc25C activity during mitosis does not require cyclin B Cdc 2 activity . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
CDC25A , CDC25B and CDC25C belong to a family of protein phosphatases which activate the cyclin dependent kinase at different points of the cell cycle . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Vanadate also increased p 21 and Chk 1 levels and reduced Cdc25C expression , leading to phosphorylation of Cdc 2 and a slight increase in cyclin B 1 expression as analyzed by Western blot . ^^^ Several regulatory pathways are involved : ( 1 ) activation of p 21 , ( 2 ) an increase of Chk 1 expression and inhibition of Cdc25C , which results in phosphorylation of Cdc 2 and possible inactivation of cyclin B1 / Cdc2 complex . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This was associated with disruption of cell cycle related events , including hyperphosphorylation and inhibition of p 34 ( cdc 2 ) kinase activity , phosphorylation of cdc25C , and alteration in cyclin B 1 expression . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Recent studies indicate that Chk 1 is also involved in the physiological G ( 2 ) phase arrest of immature Xenopus oocytes via direct phosphorylation and inhibition of Cdc25C , the activator of cyclin B Cdc 2 kinase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
At the G ( 2 ) / M transition of the cell cycle , the cdc25c phosphatase dephosphorylates inhibitory residues of cdc 2 , and cyclin B cdc 2 kinase ( MPF ) is activated . ^^^ Phosphorylation of cyclin B 1 induces its nuclear accumulation , and , since cdc25c is also believed to accumulate and activate shortly before G ( 2 ) / M in the nucleus , it has been proposed that this induces cyclin B 1 cdc2 kinase activation . ^^^ We demonstrate that cyclin B 1 phosphorylation has another essential function in vivo : it is required for cdc25c and MPF activation , which does not require nuclear accumulation of cyclin B 1 , and occurs in the cytoplasm . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
These results suggest that phosphorylation of p 21 ( Cip1 / Waf1 ) and Cdc25C and biphasic regulation of cyclin B 1 associated kinase activity may contribute to NCTD induced M phase cell cycle arrest . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Systematic mutagenesis of the DRS enabled a consensus to be derived , and it is notable that this sequence is compatible with cell cycle gene homology region sequences associated with cell cycle dependent elements in the cyclin A , cdc 2 , and CDC25C promoters . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The underlying mechanism of G 2 M arrest showed a strong inhibitory effect of combination on cdc25C , cdc2 / p34 , and cyclin B 1 protein expression and cdc2 / p34 kinase activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This block is abolished when HuCdc 6 is expressed together with a constitutively active Cyclin B / CDK1 complex or with Cdc25B or Cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The increased sensitivity to etoposide induced G ( 2 ) M arrest was associated with disruption of cell cycle related events , including hyperphosphorylation of p 34 ( cdc 2 ) kinase , change in cdc25C expression and phosphorylation , and alteration in cyclin B 1 expression . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We present evidence to show that 2 ME preferentially inhibits the growth of medulloblastoma cells significantly by blocking cell cycle progression predominantly in G ( 2 ) / M phase . 2 ME treatment results in phosphorylation of cdc25C without any significant alterations in the expression of cyclin B 1 or p34cdc2 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Both down regulation of cell cycle regulators ( p34cdc2 , cyclin B 1 and cdc25C ) and decreased association between p34cdc2 and cyclin B 1 were found to be associated with the activity of cdc 2 kinase abated after the H . pylori infection . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The RRASK motif in Xenopus cyclin B 2 is required for the substrate recognition of Cdc25C by the cyclin B Cdc 2 complex . ^^^ We show that this conserved sequence in Xenopus cyclin B 2 , termed the RRASK motif , is required for the substrate recognition by the cyclin B Cdc 2 complex of Cdc25C . ^^^ Cdc 2 bound to the cyclin B 2 RRASK mutant was not dephosphorylated by Cdc25C , and as a result , the complex was inactive . ^^^ The cyclin B 2 RRASK mutants can form a complex with the constitutively active Cdc 2 , but a resulting active complex did not phosphorylate a preferred substrate Cdc25C in vitro , although it can phosphorylate the non specific substrate histone H 1 . ^^^ The RRASK mutations prevented the interaction of Cdc25C with the cyclin B 2 Cdc2 complex . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Our results show that the mRNA and protein levels of the G ( 2 ) / M regulators cdc 2 , cyclin B 1 and cdc25C are down regulated in MCF7TDX cells , while unaffected in MCF 7 cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S phase , we investigated whether cdc25C was required for S phase transit . ^^^ Here , we show that in both nontransformed human fibroblasts and HeLa cells , cdc25C protein levels significantly increased concomitant with S phase onset and cyclin A synthesis . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
A stronger G 2 M arrest by these combinations was accompanied by a substantial decrease in the levels of cdc 2 , cyclin B 1 and cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Statistically significant reduction in the expression of several proteins that regulate G2 / M progression , including cyclin B 1 , cell division cycle ( Cdc ) 25B and Cdc25C ( 44 , 45 and 90 % reduction , respectively , compared with control ) , was also observed in the tumors of AITC treated mice relative to control tumors . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Together with the recently described mouse cdc25C promoter , human cyclin B 2 is the second identified gene which solely requires a CHR for its cell cycle regulation . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We examined the ATM , chk 2 , CDC25C , cdc 2 , and cyclin B 1 protein expression by immunohistochemistry . ^^^ Elevated or reduced expression rates of ATM , chk 2 , CDC25C , cdc 2 and cyclin B 1 were 4 . 5 % ( 1 / 22 ) , 0 % , 0 % , 0 % and 4 . 5 % ( 1 / 22 ) in EH and 3 . 3 % ( 2 / 61 ) , 4 . 9 % ( 3 / 61 ) , 13 . 1 % ( 8 / 61 ) , 9 . 8 % ( 6 / 61 ) and 9 . 8 % ( 6 / 61 ) in EC . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Many key cell cycle regulators such as p 53 , Cdc25C , cyclin B , and components of the anaphase promoting complex are directly targeted by Plks . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cells were arrested in G2 / M phase after 15 deoxy PGJ 2 treatment with concomitant increase in the expression of G2 / M phase regulatory protein cyclin B 1 but decrease in the expression of cdk 2 , cdk 4 , cyclin A , cyclin D 1 , cyclin E , and cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In other studies , we observed that G2 / M arrest by silibinin was associated with a decrease in cdc25C , cdc2 / p34 and cyclin B 1 protein levels , as well as cdc2 / p34 kinase activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In addition , salvinal treatment resulted in upregulation of cyclin B 1 levels , activation of Cdc 2 kinase , and Cdc25c phosphorylation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We find that Vpr directly inhibits the in vitro activity of a phosphatase , Cdc25C , which normally activates Cyclin B 1 p34Cdc2 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Transient transfection assays show that YA aa inhibits transcription from the NF Y target promoters , such as cdc 2 , cyclin A , and cdc25C . ^^^ We constructed a recombinant adenovirus encoding YA aa and found that YA aa expression leads to repression of cell cycle regulatory genes , such as cyclin A , RNR R 2 , DNA polymerase alpha , cdc 2 , cyclin B , and cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Mutation of a CK 2 phosphorylation site in cdc25C impairs importin alpha / beta binding and results in cytoplasmic retention . cdc25C is a phosphatase , which activates the mitosis promoting factor cyclin B1 / cdc2 by dephosphorylation , and thus triggers G ( 2 ) / M transition . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cell cycle arrest induced by SFN was associated with a significant decrease in protein levels of cyclin B 1 , cell division cycle ( Cdc ) 25B , and Cdc25C , leading to accumulation of Tyr 15 phosphorylated ( inactive ) cyclin dependent kinase 1 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Further , the G ( 2 ) / M arrest by silibinin in TCC SUP cells was associated with a decrease in pCdc25c ( Ser 216 ) , Cdc25c , pCdc 2 ( Tyr 15 ) , Cdc 2 and cyclin B 1 protein levels . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Measurements of cyclin A and B protein levels , Cdk 2 and Cdc 2 kinase activities , Cdc25C phosphorylation , and Chk 1 kinase activity were consistent with UCN 01 induced abrogation of the S / G2 phase checkpoint in ara C treated cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Additional studies indicate that the effect of BPR0L075 on cell cycle arrest is associated with an increase in cyclin B 1 levels and a mobility shift of Cdc 2 and Cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
We found that overreplication caused by depletion of geminin activated both Chk 1 and Chk 2 , which then phosphorylated Cdc25C on Ser 216 , resulting in its sequestration outside the nucleus , thus inhibiting cyclin B Cdc 2 activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The two subunits of MPF , p 34 ( cdc 2 ) and cyclin B , were shown to be included in the substrates phosphorylated by PKC in fertilized mouse eggs , while PKC modulator affected the electrophoretic mobility shift of cdc 2 and cdc25C by dephosphorylation and phosphorylation . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Expression of wee 1 and the correlated cell cycle components , namely cdc 2 , cyclin B 1 , and cdc25C , was evaluated by immunohistochemistry . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Active Plk 1 is involved in promotion of mitotic entry through activation of Cdc25C , and through nuclear import of cyclin B 1 that together activate Cdc2 / cyclin B kinase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
G 2 M arrest was associated with a decrease in Cdc25C , Cdc2 / p34 and cyclin B 1 , which were more prominent in LNCaP compared to DU 145 cells . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Many key cell cycle regulators such as p 53 , Cdc25C , cyclin B , components of the anaphase promoting complex , and mitotic motor proteins are directly targeted by Plks . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In both arsenical compounds treated cells , the protein levels of cyclin A and CDC25C were significantly reduced in a dose dependent manner , concomitant to the reduced activities of CDK 2 and CDC 2 associated kinase . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The expression of cdc25c and cyclin B 1 and cdc2 / p34 associated H 1 histone kinase activity also decreased in HYAL 1 AS transfectants . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Chromatin immunoprecipitation experiments indicated that p 53 is associated with cyclin B 2 , CDC25C , and Cdc 2 promoters in vivo before and after DNA damage , requiring DNA bound NF Y . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This change is associated with a marked reduction in regulators of S ( cyclin A , cyclin dependent kinase ( cdk ) 2 ) , G2 / M ( cyclin B 1 , cdk 1 , cdc25c ) and G 1 ( cyclin D 1 , cyclin E , cdk 2 , cdk 4 ) phase , and an increase in p 21 and p 27 level . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Finally , the combination of beta elemene and cisplatin was found to increase the phosphorylation of Cdc 2 and Cdc25C , which leads to a reduction in Cdc 2 cyclin B 1 activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Analysis of a battery of cell cycle proteins ( cyclin D 1 , cdk 5 , PCNA , cdc25A and cdc25C ) indicated exposure specific differential expression profiles . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In this study , we have studied the ability of MG to cause DNA damage , cell cycle arrest in mimosine synchronised and the possible roles of Chk 1 , Chk 2 , Cdc 2 , Cdc25C , 14 3 3 and Cyclin B 1 in control and MG transformed SHE cells in order to understand the differential mechanisms associated with G2 / M checkpoint control . ^^^ Also a decrease in Cdc25C , 14 3 3 and Cyclin B 1 was observed in MG treated transformed cells , whereas MG treated control cells showed elevated levels . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Curcumin induced cell cycle arrest by reducing the expression of cyclin D 1 , Cdk 1 and Cdc25C and apoptosis by reducing the expression of XIAP and survivin . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
HYAL 1 AS transfectants had a G 2 M block due to decreased cyclin B 1 , cdc25c , and cdc2 / p34 expression and cdc2 / p34 kinase activity . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Treatment of cancer cells with MMPT and DBPT led to a time dependent accumulation of cells arrested in the G2 / M phase with modulation of the expression of proteins such as cyclin B 1 , cdc25C , and phosphorylated histone H 3 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
These findings indicate that silymarin and silibinin modulate G 1 phase cyclins CDKs CDKIs for G 1 arrest , and the Chk 2 Cdc25C Cdc2 / cyclin B 1 pathway for G 2 M arrest , together with an altered subcellular localization of critical cell cycle regulators . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NF Y transcription factor binds to CCAAT boxes on promoters of cell cycle regulatory genes such as cdc 2 , cyclin B , cdc25C , and cyclin A . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Treating LNCaP and DU 145 cells with DBPT led to a time dependent cell cycle arrest in the G ( 2 ) / M phase and increased levels of G ( 2 ) / M checkpoint proteins , such as cyclin B 1 , cdc25C , phosphorylated histone H ( 3 ) , and MPM 2 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Analysis of the cell cycle regulatory proteins demonstrated that squamocin did not change the steady state levels of Cdk 2 , Cdk 4 , cyclin A , cyclin B 1 , cyclin D 3 and cyclin E , but decreased the protein levels of Cdk 1 and Cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
AE increased the levels of Wee 1 and cdc25c , and may have led to inhibition of the levels of cyclin dependent kinase 1 and cyclin B 1 , which cause G2 / M arrest . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In this study , under treatment with various concentrations of PA in MDA MB 231 cell line , we checked mRNA levels for cyclin A and cyclin B 1 and the protein levels of cyclin A and cyclin B 1 , Cdc 2 ( cyclin dependent kinases ) , p 21 ( waf1 / cip1 ) and P 27 ( Kip 1 ) ( cyclin dependent kinase inhibitors ) , Cdc25C , Chk 2 and Wee 1 kinase ( cyclin dependent kinase relative factors ) in cell cycle G2 / M phase . ^^^ From those results , we determined that PA arrests MDA MB 231 cells at the G2 / M phase by ( 1 ) inhibiting synthesis or stability of mRNA and their downstream protein levels of cyclin A and cyclin B 1 , ( 2 ) increasing p 21 ( waf1 / cip1 ) and P 27 ( kip 1 ) levels , ( 3 ) increasing Chk 2 , thus causing an increase in Cdc25C phosphorylation / inactivation and inducing a decrease in Cdc 2 levels and an increase in Wee 1 level . ^^^ According to the results obtained , PA appears to possess anticarcinogenic properties ; these results suggest that the effect of PA on the levels of phosphorylated / inactivated Cdc25C are mediated by Chk 2 activation , at least in part , via p 21 ( waf1 / cip1 ) and P 27 ( kip 1 ) cyclin dependent kinase inhibitors pathway to arrest cells at G2 / M phase in breast cancer carcinoma cells . . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cell cycle arrest caused by ITC occurs mainly in the G2 / M phase , and both the G 2 and M phases are targetted ; critical G 2 phase regulators , including cyclin B 1 , cell division cycle ( Cdc ) 2 and Cdc25C , are down regulated or inhibited , and tubulin polymerization and spindle assembly are disrupted . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
This effect was associated with a reduction in the expression of regulatory proteins of G ( 2 ) M ( cyclin dependent kinase 1 and cdc25c ) and S ( cyclin A ) phases , and with an increase in the expression of apoptosis related proteins , such as p 53 and p 21 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
However , other G2 / M regulatory molecules , such as cyclin A , Cdc 2 , Cdk 2 , Wee 1 and Cdc25C , were not modulated by curcumin treatment . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Two dimensional electrophoresis in conjunction with matrix assisted laser desorption / ionization time of flight ( MALDI TOF ) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC induced apoptosis is accompanied by increased levels of caspase 1 , checkpoint suppressor 1 , caspase 4 , NF kappaB inhibitor , AP 2 , c Jun N terminal kinase , melanoma inhibitor , granzyme K , G1 / S specific cyclin D 3 , cystein rich protein , Ras related protein Rab 37 or Ras related protein Rab 13 , and reduced levels of EMS ( oncogene ) , ATP synthase , tyrosine phosphatase , Cdc25c , 14 3 3 protein or specific structure of nuclear receptor . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
In the present study , the effects of the naturally occurring berberine ( the major constituent of Coptis chinensis ) on the cell cycle , as well as on CDK 1 , cyclin B 1 , 14 3 3sigma , Wee 1 and Cdc25c expressions , were investigated in the human promyelocytic leukemia HL 60 cells and in the murine myelomonocytic leukemia WEHI 3 cells . ^^^ The berberine induced G2 / M phase arrest in both examined cell lines was accompanied by increased levels of Wee 1 and 14 3 3sigma , but decreased levels of Cdc25c , CDK 1 and cyclin B 1 . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Various mitotic markers , including MPM 2 , cdc25c , cyclin B 1 , or phosphorylated histone H 3 , were investigated to explore the molecular consequences of Plk 1 depletion . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Cell cycle arrest induced by LOR ( 75 microM , 24 h ) was associated with a significant decrease in protein levels of cyclin B 1 , cell division cycle ( Cdc ) 25B , and Cdc25C , leading to accumulation of Tyr 15 phosphorylated Cdc 2 ( inactive form ) . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
A treatment of MCF 7 cells with 30 microM CAI caused a time dependent decrease in the levels of proteins that regulate G ( 2 ) / M progression , including Cdk 1 , Cyclin B 1 , and Cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
On the contrary , a decline of cyclin Bl , cdc 2 and cdc25C mRNA and protein expression in SAS cells was found after exposure to sodium butyrate . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
G2 / M arrest was correlated with increased p21 / WAF1 levels , downregulation of the checkpoint proteins cyclin B1 / cdc2 and mobility shift of cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
Following LPS induced vascular injury , FoxM 1 CKO lungs demonstrated impaired cell proliferation in association with sustained expression of p 27 ( Kip 1 ) and decreased expression of cyclin B 1 and Cdc25C . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
The expression of cyclin A , cyclin B 1 , cdk 1 , and cdc25C , as well as the cdk 1 associated kinase activities , is upregulated after DNA damage , provoking a mutant p53 / NF Y dependent increase in DNA synthesis . ^^^
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA
Interacting proteins: P30307 and P12004	Pubmed	SVM Score :0.0
NA