Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.77433357
The checkpoint kinases , Chk 1 and Cds 1 , are proposed to regulate the interactions between human Cdc25C and 14 3 3 proteins by phosphorylating Cdc25C on serine 216 . 14 3 3 proteins , in turn , function to keep Cdc25C out of the nucleus . 0.77433357^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 phosphorylates the dual specificity phosphatase cdc25C on Ser 216 , and this may be involved in preventing cdc 25 from activating cdc2 / cyclinB and initiating mitosis . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Cdc 25 mitotic inducer targeted by chk 1 DNA damage checkpoint kinase . ^^^ The kinase Wee 1 and the phosphatase Cdc 25 , which regulate Cdc 2 phosphorylation , were evaluated as targets of Chk 1 , a kinase essential for the checkpoint . ^^^ Fission yeast cdc 2 3w Deltacdc 25 cells , which express activated Cdc 2 and lack Cdc 25 , were responsive to Wee 1 but insensitive to Chk 1 and irradiation . ^^^ Expression of large amounts of Chk 1 produced the same phenotype as did loss of the cdc 25 gene in cdc 2 3w cells . ^^^ Cdc 25 associated with Chk 1 in vivo and was phosphorylated when copurified in Chk 1 complexes . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The repair checkpoint kinase Chk 1 regulates Cdc 25 , a phosphatase that activates Cdc 2 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Recombinant Xenopus Chk 1 ( Xchk 1 ) phosphorylates the mitotic inducer Cdc 25 in vitro on multiple sites including Ser 287 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Replication checkpoint requires phosphorylation of the phosphatase Cdc 25 by Cds 1 or Chk 1 . ^^^ The Chk 1 kinase , an effector of the DNA damage checkpoint , phosphorylates Cdc 25 , an activator of Cdc 2 . ^^^ We show by mutagenesis that Chk 1 functions redundantly with the kinase Cds 1 at the replication checkpoint and that both kinases phosphorylate Cdc 25 on the same sites , which include serine residues at positions 99 , 192 and 359 . ^^^ We conclude that both Cds 1 and Chk 1 regulate the binding of Cdc 25 to 14 3 3 proteins as part of the checkpoint response to unreplicated DNA . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The protein Chk 1 enforces this checkpoint by phosphorylating the mitotic inducer Cdc 25 . ^^^ Phosphorylation of Cdc 25 by Chk 1 creates a binding site in Cdc 25 for 14 3 3 proteins , but it is not known how 14 3 3 proteins regulate Cdc 25 . ^^^ Activation of the DNA damage checkpoint causes the net nuclear export of Cdc 25 by a process that requires Chk 1 , Rad 24 and nuclear export machinery . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Our results suggest that in fission yeast the interaction between the 14 3 3 proteins and Cdc 25 does not require Chk 1 function and is unaffected by DNA damage , in sharp contrast to the interaction between the 14 3 3 proteins and Chk1 . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Cdc 25 inhibited in vivo and in vitro by checkpoint kinases Cds 1 and Chk 1 . ^^^ Chk 1 , a protein kinase that is required for the G 2 M damage checkpoint that prevents mitosis while DNA is being repaired , also inhibited Cdc 25 in the in vitro assay . ^^^ In vitro , Cds 1 and Chk 1 phosphorylated Cdc 25 predominantly on serine 99 . ^^^ Thus , Cds 1 and Chk 1 seem to act in different checkpoint responses to regulate Cdc 25 by similar mechanisms . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
In other systems , Chk 1 kinase phosphorylates and suppresses the activity of Cdc 25 phosphatase : the resulting failure to remove inhibitory phosphate from cyclin dependent kinase 1 ( Cdk 1 ) prevents entry into mitosis [ 4 ] [ 5 ] . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
A detailed model of the G ( 2 ) DNA damage checkpoint ( G2DDC ) system is presented that includes complex regulatory networks of the mitotic kinase Cdc 2 , phosphatase Cdc 25 , Wee 1 kinase , and damage signal transduction pathways involving Chk 1 and p 53 . ^^^ The detailed model could be used to explain various experiments relevant to G2DDC reported recently , including the nuclear export of 14 3 3 bound Cdc 25 , the down regulation of cyclin B 1 expression by p 53 , the effect of Chk 1 and p 53 on Cdc 25 levels , and Wee 1 degradation . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 regulates the activity and localization of Cdc 25 , the tyrosine phosphatase that activates the cdk Cdc 2 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Other potentially useful cell cycle areas for exploration include cyclin CDK interactions , Cdc 25 activation of cyclin CDK complexes , ubiquitin mediated proteolysis of cyclins , cell cycle check point kinases like Chk 1 , and recently identified oncogenic cell cycle related aurora and polo like kinases . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 , a nuclear DNA damage / replication G 2 checkpoint kinase , phosphorylates Cdc 25 and causes its nuclear exclusion in yeast and mammalian cells , thereby arresting the cell at the G 2 phase until DNA repair / replication is completed . ^^^ Chk 1 is also involved , at least in part , in the natural G 2 arrest of immature Xenopus oocytes , but it is unknown how Chk 1 inhibits Cdc 25 function and undergoes regulation during oocyte maturation . ^^^ By using enucleated oocytes , we show here that Chk 1 inhibits Cdc 25 function in the cytoplasm of G 2 arrested oocytes and that Cdc 25 is activated exclusively in the cytoplasm of maturing oocytes . ^^^ Based on these results , we discuss the possibility that Chk 1 ( with the basal activity ) may function as an ordinary regulator of Cdc 25 in oocytes ( and in other cell types ) and that Chk 1 might be hyperactivated in response to the G 2 checkpoint via its dramatic conformational change . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
DNA damage : Chk 1 and Cdc 25 , more than meets the eye . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
This checkpoint is enforced by Chk 1 , a protein kinase that targets Cdc 25 [ 2 7 ] . ^^^ We propose that direct inhibition of Cdc 25 phosphatase activity by Chk 1 , as demonstrated in vitro with fission yeast and human Chk 1 [ 15 , 16 ] , is sufficient for proficient checkpoint regulation of Cdc 25 and may be the primary mechanism of checkpoint enforcement in fission yeast . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 and Chk 2 inhibit Cdc 2 by inactivating Cdc 25 , the phosphatase that normally activates Cdc 2 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
This checkpoint is enforced by Chk 1 , a protein kinase that inhibits the mitotic inducer Cdc 25 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
We demonstrate that ( 1 ) CDC25B and C efficiently replace Cdc 25 for vegetative growth , ( 2 ) CDC25C is able to restore a functional checkpoint in response to ionizing radiation in both a Chk 1 and Cds 1 dependent manner , ( 3 ) CDC25B and C are equally efficient in the response to UV irradiation , CDC25B being only dependent on Chk 1 , while CDC25C depends on both Chk 1 and Cds 1 , and ( 4 ) CDC25C is able to restore a functional DNA replication checkpoint induced by hydroxyurea in a Cds 1 dependent manner . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
For this we studied the effect of CA4P on selected markers of apoptosis [ caspases 9 and 3 , poly ( ADP ribose ) polymerase ( PARP ) , bcl 2 , and bax ] and G 2 M protein regulators ( p 53 , MDM 2 , 14 3 3sigma , GADD 45 , cdc 2 , cdc 25 , chk 1 , wee 1 , p 21 , and cyclin B 1 ) . ^^^ Exposure of WSU CLL cells to 4 and 5 nM CA4P was associated with overproduction of total p 53 and no dramatic change in MDM 2 , 14 3 3sigma , GADD 45 , the cyclin dependent kinase cdc 2 , its inhibitory phosphorylation , the cdc 2 inhibitory kinase ( wee 1 ) , chk 1 , or cdc 25 hyperphosphorylation . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 phosphorylation , decrease of Cdc 25 A levels and S phase arrest were abolished by caffeine , demonstrating that active checkpoint signaling rather than passive mechanical blockage by ICLs causes the PUVA induced replication arrest . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The G 2 DNA damage checkpoint delays mitotic entry via the upregulation of Wee 1 kinase and the downregulation of Cdc 25 phosphatase by Chk 1 kinase , and resultant inhibitory phosphorylation of Cdc 2 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The DNA damage checkpoints employ damage sensor proteins , such as ATM , ATR , the Rad 17 RFC complex , and the 9 1 1 complex , to detect DNA damage and to initiate signal transduction cascades that employ Chk 1 and Chk 2 Ser / Thr kinases and Cdc 25 phosphatases . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 , but not Chk 2 , inhibits Cdc 25 phosphatases by a novel common mechanism . ^^^ A C terminal site presumably equivalent to Thr 504 exists in all known Cdc 25 family members from yeast to humans , and its phosphorylation by Chk 1 ( but not Chk 2 ) can also inhibit all examined Cdc 25 family members from C terminally interacting with their Cdk cyclin substrates . ^^^ Thus , Chk 1 but not Chk 2 seems to inhibit virtually all Cdc 25 phosphatases by a novel common mechanism . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Erythropoietin or IL 3 significantly enhanced etoposide induced activation specific phosphorylation of Chk 1 , a checkpoint kinase that inhibits Cdc 2 activation by Cdc 25 phosphatases , and led to the inhibition of Cdc 2 kinase activity with the persistent inhibitory phosphorylation on Tyr 15 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 and Chk 2 kinases regulate Cdc 25 , Wee 1 and p 53 that ultimately inactivate cyclin dependent kinases ( Cdks ) which inhibit cell cycle progression . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Members of the eukaryotic Cdc 25 phosphatase family are key targets of the Chk 1 and Chk 2 checkpoint kinases , which inactivate Cdc 25 to halt cell cycle progression when DNA is damaged or incompletely replicated . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
C TAK 1 is ubiquitously expressed in human tissues and cell lines and is distinct from the DNA damage checkpoint kinase Chk 1 , shown previously to phosphorylate Cdc25C on serine 216 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
This is the same site phosphorylated by the protein kinase Chk 1 , which suggests that , in response to DNA damage and DNA replicational stress , Chk 1 and Chk 2 may phosphorylate Cdc25C to prevent entry into mitosis . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 kinase , a DNA damage / replication G 2 checkpoint kinase , has recently been shown to phosphorylate and inhibit Cdc25C , a Cdc 2 Tyr 15 phosphatase , thereby directly linking the G 2 checkpoint to negative regulation of Cdc 2 . ^^^ Moreover , when ectopically expressed in oocytes , a Chk 1 nonphosphorylatable Cdc25C mutant alone can induce prophase 1 release much more efficiently than wild type Cdc25C ; if endogenous Chk 1 function is inhibited , however , even wild type Cdc25C can induce the release very efficiently . ^^^ These results suggest strongly that Chk 1 is involved in physiological prophase 1 arrest of Xenopus oocytes via the direct phosphorylation and inhibition of Cdc25C . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Using mass spectrometry , we found that , similar to Chk 1 , Chk 2 can phosphorylate serine 216 in Cdc25C , a site known to be involved in negative regulation of Cdc25C . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Further studies reveal that His 6 Prk phosphorylates Cdc25C on serine 216 , a residue also phosphorylated by Chk 1 and Chk 2 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
We have developed a quantitative assay for Chk 1 activity , using a peptide derived from a region of Xenopus Cdc25C containing Ser 287 , a known target of Chk 1 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Molecular modeling of the interaction of a Cdc25C peptide with Chk 1 has uncovered several conserved residues that are important for substrate selectivity . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
We show that p 21 blocks the activating phosphorylation of Cdc 2 on Thr ( 161 ) . p 21 does not interfere with the dephosphorylation of two inhibitory phosphorylation sites on Cdc 2 , Thr ( 14 ) and Tyr ( 15 ) , indicating that p 21 targets a different event in Cdc 2 activation as the well described DNA damage checkpoint pathway involving Chk 1 and Cdc25C . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
In addition , we observed an increase in chk 1 kinase and a decrease in cdc25C protein levels . ^^^ Chk 1 phosphorylates cdc25C on serine 216 and inactivates it whereas cdc25C dephosphorylates tyrosine 15 phosphate of cdc 2 and activates the cdc 2 cyclin B complex . ^^^ Therefore , the increase in chk 1 and the decrease in cdc25C both participate in inhibiting the G2 / M transition . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Both Chk 1 and Chk 2 , the checkpoint kinases , can phosphorylate Cdc25C and inactivate its in vitro phosphatase activity . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
U87MG glioma cells treated with TMZ underwent G ( 2 ) M arrest associated with Chk 1 activation and phosphorylation of both cdc25C and cdc 2 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Vanadate also increased p 21 and Chk 1 levels and reduced Cdc25C expression , leading to phosphorylation of Cdc 2 and a slight increase in cyclin B 1 expression as analyzed by Western blot . ^^^ Several regulatory pathways are involved : ( 1 ) activation of p 21 , ( 2 ) an increase of Chk 1 expression and inhibition of Cdc25C , which results in phosphorylation of Cdc 2 and possible inactivation of cyclin B1 / Cdc2 complex . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The Chk 1 Cdc25C regulation is involved in sensitizing A 253 cells to a novel topoisomerase 1 inhibitor BNP 1350 by bax gene transfer . ^^^ A chk 1 activity assay was performed with GST cdc25C ( 200 256 ) or GST cdc25C ( S216A ) ( 200 256 ) fusion proteins as the substrate . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Recent studies indicate that Chk 1 is also involved in the physiological G ( 2 ) phase arrest of immature Xenopus oocytes via direct phosphorylation and inhibition of Cdc25C , the activator of cyclin B Cdc 2 kinase . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The optimal motifs are similar for both kinases and most closely resemble the previously identified Chk 1 and hCds1 / Chk2 substrate target sequences in Cdc25C and Cdc25A , the regulation of which plays an important role in S and G ( 2 ) M arrest . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The suppression occurred despite normal induction of p 53 and normal phosphorylation of p 53 at S 20 and Cdc25C at S 216 the two known substrates of Chk 1 kinase activity . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Pharmacological inhibition of Chk 1 activity or a mutant of Cdc25C that lacks the Chk 1 phosphorylation site still undergoes degradation in response to oxidants . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Somatic frameshift mutations were observed in hMLH 3 , hMSH 3 , hMSH 6 , CHK 1 , and BAX genes in MSI H endometrial hyperplasias and cancers , whereas mutations in ATR and CDC25C were observed only in MSI H ECs . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 blocks the activation of the Cdc 2 cyclin B kinase complex , and hence entry into mitosis , by disrupting the translocation of the phosphatase Cdc25C from the cyotoplasm to the nucleus . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Increased chk 1 phosphorylation at Ser 345 induced by SN 38 was accompanied by the observed G 2 phase arrest in the A 253 cell line , while significant downregulation of chk 1 and cdc25C phosphorylation , which resulted in the abrogation of G2 / M checkpoint arrest , was noted in FaDu cells at this timepoint . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The interaction between CK2beta and Chk 1 leads to an increase in the Cdc25C phosphorylation activity of Chk 1 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Finally , CDC25A , CDC25B , a dominant negative CHK 1 , but not CDC25C or a dominant negative WEE 1 , stimulated histone H 3 phosphorylation after DNA damage . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
In vertebrates , Cdc25C is inhibited by phosphorylation at a single site targeted by the checkpoint kinases Chk 1 and Cds1 / Chk2 in response to DNA damage or replication arrest . ^^^ CaMKII phosphorylates Cdc25C efficiently on S 287 in vitro and , like Chk 1 , is inhibited by 7 hydroxystaurosporine ( UCN 01 ) and debromohymenialdisine , compounds that abrogate G 2 arrest in somatic cells . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Unlike the full length protein , C terminally truncated CHK 1 displays autophosphorylation , phosphorylates CDC25C on Ser ( 216 ) , and delays cell cycle progression . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Colony forming assays , flow cytometric DNA analysis , immunoblotting ( Chk 1 , Chk 2 , Cdc25C , Cdk 1 , 14 3 3 , p 53 , p 21 ) , and protein kinase assays ( ILK , PKBalpha / Akt , GSK 3beta ) were performed with or without PI3K inhibition using LY 294002 or wortmannin . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Measurements of cyclin A and B protein levels , Cdk 2 and Cdc 2 kinase activities , Cdc25C phosphorylation , and Chk 1 kinase activity were consistent with UCN 01 induced abrogation of the S / G2 phase checkpoint in ara C treated cells . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The p 38 inhibitor SB 203580 or the Chk 1 inhibitor UCN 01 or their combination blocked TMZ mediated inactivation of cdc25C and cdc 2 , suggesting that p 38 and Chk 1 pathways work cooperatively and are both necessary to inactivate cdc25C and cdc 2 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
The basic mechanism of this assay is to observe the phosphorylated levels of a fragment of CDC25C containing the site that can be phosphorylated by CHK 1 in vitro . ^^^ Because CHK 1 and CHK 2 share some substrates such as CDC25C in vitro , this assay could also be used for CHK 2 activity assay , except that the CHK 2 antibody will replace the CHK 1 antibody . . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Within 24 hours of treatment , HMJ 38 influenced the CDK / cyclin B activity by increasing Chk 1 , Wee 1 and p 21 and decreasing Cdc25C protein levels . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
We found that overreplication caused by depletion of geminin activated both Chk 1 and Chk 2 , which then phosphorylated Cdc25C on Ser 216 , resulting in its sequestration outside the nucleus , thus inhibiting cyclin B Cdc 2 activity . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Slowing follows caffeine sensitive activation of the checkpoint kinase , Chk 1 ; degradation of the cell cycle phosphatase , Cdc25A ; and inhibitory phosphorylation of Cdc25C and cyclin dependent kinases ( Cdks ) . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Proteasome mediated degradation of Cdc25A and phosphorylation of Cdc25C were induced by Ara C treatment , presumably due to the activation of Chk 2 and Chk 1 , respectively . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Results indicate that BRCA 1 induced G2 / M cell cycle arrest and ERK1 / 2 activation correlate with changes in the level and / or activity of several key regulators of the G2 / M checkpoint , including activation of Chk 1 and Wee 1 kinases , induction of 14 3 3 , and down regulation of Cdc25C . ^^^ Biochemical studies established that ERK1 / 2 inhibition abolished the effects of BRCA 1 on components of the G2 / M checkpoint , including regulation of Cdc25C expression and activation of Wee 1 and Chk 1 kinases . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Chk 1 phosphorylates Cdc25C at serine 216 , a major regulatory site , in response to DNA damage . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Temozolomide treated control cells activated the DNA damage signal transducers Chk 1 , Chk 2 , and p 38 , leading to Cdc25C and Cdc 2 inactivation , prolonged G 2 arrest , and loss of clonagenicity by a combination of senescence and mitotic catastrophe . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Further upstream , we observed that resveratrol causes phosphorylation of cell division cycle 25C ( Cdc25C ) tyrosine phosphatase via the activation of checkpoint kinases Chk 1 and Chk 2 , which in turn were activated via ATM ( ataxia telangiectasia mutated ) / ATR ( ataxia telangiectasia Rad 3 related ) kinase in response to DNA damage , as resveratrol also increased phospho H2A . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
In this study , we have studied the ability of MG to cause DNA damage , cell cycle arrest in mimosine synchronised and the possible roles of Chk 1 , Chk 2 , Cdc 2 , Cdc25C , 14 3 3 and Cyclin B 1 in control and MG transformed SHE cells in order to understand the differential mechanisms associated with G2 / M checkpoint control . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
Previous work identified the cdc 2 activating phosphatase cdc25C and the cdc 2 inhibitory kinase wee 1 as targets of the incomplete replication induced kinase Chk 1 . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
We further demonstrated that the LOR induced Cdc25C ( Ser 216 ) phosphorylation was blocked in the presence of checkpoint kinase 1 ( Chk 1 ) specific inhibitor ( SB 218078 ) . ^^^ These results implied that Chk 1 mediated phosphorylation of Cdc25C plays a major role in response to LOR mediated G ( 2 ) / M arrest . ^^^
Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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Interacting proteins: P30307 and O14757	Pubmed	SVM Score :0.0
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