| Interacting proteins: Q8IYP9 and P29475 |
Pubmed |
SVM Score :0.0 |
| Embryonic development , prostaglandin E ( PGE ) generation and nitric oxide synthase ( NOS ) activity during organogenesis were evaluated in an experimental rat model of non insulin dependent diabetes ( NIDD ) generated by neonatal administration of streptozotocin . ^^^ Activity of NOS was higher in NIDD 11 day embryos than in controls . ^^^ Treatment in vivo of control and NIDD rats ( Days 7 11 of gestation ) with a NOS inhibitor ( L NAME ; 5 mg kg ( 1 ) i . p . ) reduced embryonic PGE production and induced a higher resorption rate and an increase in neural tube defects . ^^^ |
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| Interacting proteins: Q8IYP9 and P29475 |
Pubmed |
SVM Score :0.0 |
| NIDD , a novel DHHC containing protein , targets neuronal nitric oxide synthase ( nNOS ) to the synaptic membrane through a PDZ dependent interaction and regulates nNOS activity . ^^^ Here we report the identification and characterization of a novel nNOS interacting DHHC domain containing protein with dendritic mRNA ( NIDD ) ( GenBank accession number AB 098078 ) , which increases nNOS enzyme activity by targeting the nNOS to the synaptic plasma membrane in a PDZ domain dependent manner . ^^^ In vitro pull down assays suggested that the C terminal tail region of NIDD specifically interacted with the PDZ domain of nNOS . ^^^ The PDZ dependence was confirmed by an experiment using a deletion mutant , and the interaction was further confirmed by co sedimentation assays using COS 7 cells transfected with NIDD and nNOS . ^^^ Both NIDD and nNOS were enriched in synaptosome and synaptic plasma membrane fractions and were present in the lipid raft and postsynaptic density fractions in the rat brain . ^^^ |
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