| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :1.0699049 |
| In addition , PSD 95 binds with neuronal nitric oxide synthase ( nNOS ) , which is competitively inhibited by carboxy terminal PDZ ligand of nNOS ( CAPON ) and , thereby , nNOS activity is thought to be regulated by PSD 95 and CAPON . 1.0699049^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.71745007 |
| Here , we report that substitution of an Arg for Lys 165 in PSD 95 PDZ 2 disrupted its interaction with nNOS , but not with the C terminus of the Shaker type K ( + ) channel Kv1 . 4 . 0.71745007^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.51968 |
| In addition , PSD 95 binds various regulatory proteins including Src , Pyk 2 , SynGAP , and nNOS and may recruit signaling proteins to NMDA receptors . 0.51968^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Elucidation of unique structural binding motifs of NO synthases ( NOS ) , and microscopical codistribution of neuronal NOS ( nNOS ) , the major isoform of NOS expressed at the NMJ , with known synaptic proteins , i . e . , family members of the DPC , nicotinic acetylcholine receptor ( AChR ) , NMDA receptor , type 1 sodium and Shaker K ( + ) channel proteins , and linker proteins ( e . g . , PSD 95 , 43K rapsyn ) , suggests targeting and assembly of the NO signaling pathway at postsynaptic membrane components . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Here , we show that the N terminus of nNOS , which contains a PDZ protein motif , interacts with similar motifs in postsynaptic density 95 protein ( PSD 95 ) and a related novel protein , PSD 93 . nNOS and PSD 95 are coexpressed in numerous neuronal populations , and a PSD 95 / nNOS complex occurs in cerebellum . ^^^ PDZ domain interactions also mediate binding of nNOS to skeletal muscle syntrophin , a dystrophin associated protein . nNOS isoforms lacking a PDZ domain , identified in nNOSdelta / delta mutant mice , do not associate with PSD 95 in brain or with skeletal muscle sarcolemma . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Two nNOS interacting proteins were identified : the postsynaptic density proteins PSD 93 and PSD 95 . ^^^ PSD 93 , like PSD 95 , binds to nNOS and to the NMDA receptor 2B . ^^^ Given that PSD 93 and PSD 95 each contain multiple potential binding sites for nNOS and the NMDA receptor , complexes involving oligomers of PSD 93 / 95 may help account for the functional as well as the physical coupling of nNOS to NMDA receptors . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| The coupling of NMDA receptor mediated calcium influx and nNOS activation is postulated to be due to a physical coupling of the receptor and the enzyme by an intermediary adaptor protein , PSD 95 , through a unique PDZ PDZ domain interaction between PSD 95 and nNOS . ^^^ CAPON competes with PSD 95 for interaction with nNOS , and overexpression of CAPON results in a loss of PSD95 / nNOS complexes in transfected cells . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Neuronal nitric oxide synthase ( nNOS ) has a PSD 95 / Dlg / ZO 1 ( PDZ ) domain that can interact with multiple proteins . nNOS has been known to interact with PSD 95 and a related protein , PSD 93 , in brain and with alpha 1 syntrophin in skeletal muscle in mammals . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| The PDZ protein interaction domain of neuronal nitric oxide synthase ( nNOS ) can heterodimerize with the PDZ domains of postsynaptic density protein 95 and syntrophin through interactions that are not mediated by recognition of a typical carboxyl terminal motif . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Here , we find that PSD 95 assembles a postsynaptic protein complex containing nNOS and NMDA receptors . ^^^ In contrast , nNOS is recruited to this complex by a novel PDZ PDZ interaction in which PSD 95 recognizes an internal motif adjacent to the consensus nNOS PDZ domain . ^^^ This internal motif is a structured `` pseudo peptide ' ' extension of the nNOS PDZ that interacts with the peptide binding pocket of PSD 95 PDZ 2 . ^^^ Accordingly , we find that the nNOS PDZ domain can bind PSD 95 PDZ 2 and a COOH terminal peptide simultaneously . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| The second PDZ domain of postsynaptic density 95 ( PSD 95 PDZ 2 ) plays a critical role in coupling N methyl D aspartate receptors to neuronal nitric oxide synthase ( nNOS ) . ^^^ Complex formation between PSD 95 PDZ 2 and the nNOS PDZ was modelled on the basis of the crystal structure of the alpha 1 syntrophin PDZ / nNOS PDZ dimer . ^^^ We found that the prolonged loop connecting the betaB and betaC strands of PSD 95 PDZ 2 is likely to play a role in both the binding of the carboxyl terminal peptide and the nNOS beta finger . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Neuronal nitric oxide synthase ( nNOS ) is targeted to the cell membrane via interactions of its extended PDZ domain with PDZ domains of membrane associated proteins including PSD 95 and alpha 1 syntrophin . ^^^ In this work , we show that a 27 residue peptide comprising the C terminal extension of the extended nNOS PDZ domain is capable of binding to PSD 95 . ^^^ The results indicate that the C terminal extension peptide of the nNOS PDZ domain may represent a relatively independent structural unit in the mediation of the interaction between nNOS and PDZ domain containing proteins including PSD 95 and alpha 1 syntrophin . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Here , we used PDZ domains from neuronal nitric oxide synthase ( nNOS ) and postsynaptic density protein 95 ( PSD 95 ) to explore the mechanism for PDZ dimer formation . ^^^ Formation of nNOS / PSD 95 PDZ dimer requires a preformed beta finger structure from the nNOS PDZ domain . ^^^ The nNOS PDZ domain terminates with a approximately 30 residue amino acid beta finger peptide that is shown to be required for nNOS / PSD 95 PDZ dimer formation . ^^^ The flexibility of the NOS PDZ beta finger is likely to play a critical role in supporting the formation of nNOS / PSD 95 complex . ^^^ The experimental data also suggest that nNOS PDZ and the second PDZ domain of PSD 95 form a `` head to tail ' ' dimer similar to the nNOS / syntrophin complex characterized by 10 ray crystallography . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| DLC was contained in the NMDA R PSD 95 DAP neuronal nitric oxide synthase ( nNOS ) complex . ^^^ CONCLUSION : DAP interacts directly with DLC and nNOS , and links these proteins to the NMDA R PSD 95 complex . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Several alternatively spliced isoforms of the N methyl D aspartate receptor 1 ( NMDAR 1 ) subunit and the neuronal nitric oxide synthase ( nNOS ) are known , and recent studies have shown that a spliced C terminal may be responsible for the coupling of NMDAR ' s to nNOS via its PDZ domain and the postsynaptic density protein PSD 95 . ^^^ While NMDAR 1 4 may be related to receptor targeting and clustering to PSD 95 and to nNOS , our data suggest that differences in nNOS expression obviously do not directly influence gene expression of this particular NMDAR splice variant . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| We studied the expression of 5 ' nNOS mRNA forms and nNOS interacting proteins ( postsynaptic density protein 95 ; PSD 95 ) in the rat gastrointestinal tract and analyzed the more distinct localization of nNOS protein variants in the duodenum by immunohistochemistry with COOH and NH ( 2 ) terminal nNOS antibodies . 5 ' nNOS mRNA variants showed a site specific expression along the gastrointestinal tract with presence of all forms ( nNOSalpha a , b , c ; nNOSbeta ) in the muscle layer of esophagus , stomach , duodenum , longitudinal muscle layer of jejunum / ileum , proximal colon , and rectum . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| In neurons , neuronal nitric oxide synthase ( nNOS ) binds selectively to the second PDZ domain ( PDZ 2 ) of PSD 95 , thereby exhibiting physiological activation triggered via NMDA receptors . ^^^ In the present study , we examined how CaM K 2 participates in the phosphorylation by analysing the functional interaction between nNOS and PSD 95 in cells . ^^^ The results showed that PSD 95 directly promotes the nNOS phosphorylation at Ser ( 847 ) induced by endogenous CaM K 2 . ^^^ Thus PSD 95 mediates cellular trafficking of nNOS , and may be required for the efficient phosphorylation of nNOS at Ser ( 847 ) by CaM K 2 in neuronal cells . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND AND PURPOSE : Postsynaptic density ( PSD ) 93 and PSD 95 are the major membrane associated guanylate kinases ( MAGUKs ) at excitatory synapses of the brain linking the N methyl d aspartate receptor ( NMDAR ) with neuronal nitric oxide synthase ( nNOS ) , which contributes to cell death after neonatal hypoxia ischemia ( HI ) . ^^^ In the absence of PSD 93 , PSD 95 still interacted with NR2B and nNOS . ^^^ Under physiological conditions , PSD 95 , nNOS , NR2A , and NR2B were unaltered in the ( / ) pups . ^^^ However , at 24 hours after HI , protein expression of PSD 95 , nNOS , and NR2A but not NR2B was markedly higher in the ( / ) than in the ( + / + ) pups . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| At glutamatergic synapses , the scaffolding protein PSD 95 links the neuronal isoform of nitric oxide synthase ( nNOS ) to the N methyl d aspartate ( NMDA ) receptor . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Recent study has indicated that postsynaptic density protein 95 ( PSD 95 ) promotes Ca2+ / calmodulin dependent protein kinase 2 ( CaMKII ) mediated serine phosphorylation of neuronal nitric oxide synthase ( nNOS ) . ^^^ To investigate whether PSD 95 is involved in the brain ischemia induced enhancement of serine phosphorylation of nNOS by CaMKII in rat hippocampus , we examined the interactions among CaMKIIalpha , PSD 95 and nNOS , and the effects of suppression of PSD 95 expression on both the increased serine phosphorylation of nNOS and the interactions mentioned above by immunoprecipitation and immunoblotting . ^^^ The following results were observed : ( 1 ) brain ischemia increased markedly the interactions of CaMKIIalpha and nNOS with PSD 95 . ( 2 ) Intracerebroventricular infusion of PSD 95 antisense oligodeoxynucleotides , but not missense oligodeoxynucleotides or vehicle , not only significantly decreased the protein level of PSD 95 but also attenuated the elevated serine phosphorylation of nNOS and the interactions among CaMKIIalpha , PSD 95 and nNOS induced by 15 min ischemia . ^^^ These data suggested that PSD 95 is important for facilitating nNOS serine phosphorylation by CaMKII . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Further , we observed a striking enrichment of several mRNAs in the nNOS IN population , including that for the NMDA receptor subunit NR2D , the postsynaptic density protein 95 ( PSD 95 ) and the huntingtin associated protein 1 ( HAP 1 ) as well as nitric oxide synthase ( nNOS ) mRNA itself . ^^^ The higher expression levels of these molecules in nNOS IN when compared with MSN together with an association of nNOS , NR2D and HAP 1 in a protein complex with PSD 95 suggest that these proteins may be involved in protective pathways that contribute to the resistance of this interneuron population to neurodegeneration in HD . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| The PSD 95 nNOS interface : a target for inhibition of excitotoxic p 38 stress activated protein kinase activation and cell death . ^^^ Although the postsynaptic density protein PSD 95 can recruit the calcium dependent neuronal NO synthase ( nNOS ) to the mouth of the calcium permeable NMDA receptor , and depletion of PSD 95 inhibits excitotoxicity , the possibility that selective uncoupling of nNOS from PSD 95 might be neuroprotective is unexplored . ^^^ The relationship between excitotoxic stress generated NO and activation of p 38 , and the significance of the PSD 95 nNOS interaction to p 38 activation also remain unclear . ^^^ Experiments using a panel of decoy constructs targeting the PSD 95 nNOS interaction suggest that this interaction and subsequent NO production are critical for glutamate induced p 38 activation and the ensuing cell death , and demonstrate that the PSD 95 nNOS interface provides a genuine possibility for design of neuroprotective drugs with increased selectivity . . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Only CDs that lowered cholesterol levels redistributed the NMDA receptor NR2B subunit , PSD 95 ( postsynaptic density protein 95 kDa ) and neuronal nitric oxide synthase ( nNOS ) from Triton 10 100 insoluble membrane domains to soluble fractions . ^^^ Cholesterol repletion counteracted the ability of methylated beta CD to protect against NMDA toxicity , and reversed NR2B , PSD 95 and nNOS localization to Triton 10 100 insoluble membrane fraction . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| The current quantitative study demonstrates that the recruitment of neuronal nitric oxide synthase ( nNOS ) beneath N methyl D aspartate ( NMDA ) receptors , via postsynaptic density 95 ( PSD 95 ) proteins significantly enhances nitric oxide ( NO ) production . ^^^ Real time single cell fluorescence imaging was applied to measure both NO production and Ca ( 2+ ) influx in Chinese hamster ovary ( CHO ) cells expressing recombinant NMDA receptors ( NMDA R ) , nNOS , and PSD 95 . ^^^ The presence of PSD 95 enhanced the rate of NO production by 2 . 3 fold upon stimulation with 100 microm NMDA in CHO 1 ( + ) cells ( expressing NMDA R , nNOS and PSD 95 ) when compared with CHO 1 ( ) cells ( expressing NMDA R and nNOS lacking PSD 95 ) . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Here we studied the effects of prenatal morphine exposure on postsynaptic density protein 95 ( PSD 95 ) , an important cytoskeletal specialization involved in the anchoring of the NMDAR and neuronal nitric oxide synthase ( nNOS ) , of the hippocampal CA 1 subregion from young offspring at postnatal day 14 ( P 14 ) . ^^^ Collectively , the study demonstrates that maternal exposure to morphine decreases the magnitude of PSD 95 , nNOS , the phosphorylation of CREB ( Serine 133 ) , and LTD expression in hippocampal CA 1 subregion of young offspring ( e . g . , P 14 ) . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Glutamatergic mediated nitric oxide ( NO ) production occurs via the N methyl D aspartic acid ( NMDA ) postsynaptic density protein 95 ( PSD 95 ) neuronal nitric oxide synthase ( NOS 1 ) ternary complex . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| Agrin induced specializations also included coaggregates of N methyl d aspartic acid ( NMDA ) receptor , alpha sodium ( NaCh ) , or Shaker type K+ channel ( KCh ) / PSD 95 complexes , and NOS 1 . ^^^ |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P78352 |
Pubmed |
SVM Score :0.0 |
| NA |
|