| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.87366939 |
| We conclude that PIN interacts strongly with nNOS and is constitutively expressed in various brain regions . 0.87366939^^^ Expression of PIN cDNA as a fusion protein in E . coli produced a 10 kDa protein which interacted specifically with pure nNOS in an overlay assay . 0.75834^^^ Recently , a novel 89 amino acid protein , designated PIN , has been shown to interact specifically with nNOS and inhibit nNOS dimerization . 0.53796261^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.88863008 |
| Gel filtration experiments show that PIN binds to both the full length nNOS and nNOS fragment . 0.88863008^^^ |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.62579034 |
| Our data indicate that the protein inhibitor of nNOS ( PIN ) binds to protein kinase A inhibitors ( PKIs ) , which suggests that PKIs , together with PIN , might mediate the phosphorylation of nNOS by PKA . . 0.62579034^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| An antibody against constitutive neuronal NOS was used for immunocytochemistry on RCS rat retinas from postnatal ( PN ) days 3 , 7 , 10 , 14 , 35 , 70 and 281 and compared with that in the normal rats of PN days 3 , 7 , 10 , 14 , 54 and adults . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Binding of PIN destabilizes the nNOS dimer , a conformation necessary for activity . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Preparations of nitric oxide synthases from porcine cerebellum ( nNOS ) , bovine aortic endothelium ( eNOS ) and cytokine treated murine macrophages ( iNOS ) were inhibited by PN in their ability to transform arginine to citrulline and nitric oxide with IC 50 values of 15 , 28 , and 10 microM , respectively . ^^^ Destruction of the heme thiolate catalytic site was observed when nNOS was exposed to PN suggesting that the irreversible oxidation of this bond may be the common mechanism of NOS inhibition . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical studies using antibody to nNOS showed , after lead treatment at PN 21 PN30 , a reduction in neuronal size and optical density ( OD ) of nNOS+ cells . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Neuronal nitric oxide synthase ( nNOS ) , the biosynthetic enzyme for the free radical neurotransmitter nitric oxide , was found to be inhibited by the protein inhibitor of nNOS , designated PIN ( Jaffrey , S . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Neuronal NO synthase ( nNOS ) was discovered recently to interact specifically with the protein PIN ( protein inhibitor of nNOS ) [ Jaffrey , S . ^^^ The IC 50 for nNOS was 18 + / 6 microM GST PIN with 63 nM nNOS after 30 min at 37 degrees C . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| The structure of the neuronal nitric oxide synthase inhibitory protein , PIN ( protein inhibitor of nNOS ) , has been determined by NMR spectroscopy . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| We have shown , using NMR spectroscopy , that the previously identified PIN binding domain ( PINB ) of nNOS ( residues 161 245 ) adopts a random coil structure in solution . ^^^ By titrating 15N labeled PINB with unlabeled PIN , the PIN binding region of nNOS was precisely mapped to a 17 residue peptide fragment from Met 228 to His 244 of nNOS . ^^^ NMR titration experiments also showed that PIN binds to nNOS with a 1 : 2 stoichiometry . ^^^ A synthetic peptide corresponding to the identified PIN binding region of nNOS was used to study the interaction between PIN and nNOS in detail . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Recently , a novel 89 amino acid protein , designated protein inhibitor of nNOS ( PIN ) , has been shown to interact with and specifically inhibit nNOS activity . ^^^ The localization of PIN in muscle regions containing abundant nNOS protein suggests that it plays a role in the regulation of NO synthesis in skeletal muscle fibers . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NO is generated from arginine by NO synthase ( NOS ) ; the Ca2+ dependent neuronal isoform or nNOS ( expressed by neurones and inhibited by the protein inhibitor of nNOS , PIN ) , is also expressed by cultured normal melanocytes and by all malignant melanoma ( MM ) cell lines . ^^^ We studied the expression of nNOS and PIN in paraffin sections of 177 and 58 pigment cell lesions , respectively , using immunohistochemistry ; the activity of the necessary cofactor NADPH was studied in 26 frozen cases . ^^^ Normal melanocytes in situ lacked nNOS and PIN expression , but were NADPH + . ^^^ PIN was coexpressed with nNOS in 40 of 58 lesions . ^^^ We conclude that nNOS expression is induced de novo in benign and malignant pigment cell lesions which have all the requirements ( NADPH , PIN ) necessary for the production and modulation of NO . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| The structure of the protein known both as neuronal nitric oxide synthase inhibitory protein , PIN ( protein inhibitor of nNOS ) , and also as the 8 kDa dynein light chain ( LC 8 ) has been solved by 10 ray diffraction . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Protein inhibitor of neuronal nitric oxide synthase ( PIN ) is reported as the protein inhibiting neuronal nitric oxide synthase ( nNOS ) activity by preventing dimerization of nNOS . ^^^ Protein inhibitor of neuronal nitric oxide synthase ( PIN ) is reported as the protein inhibiting neuronal nitric oxide synthase ( nNOS ) activity by preventing dimerization of nNOS . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Recently , a novel 89 amino acid protein [ protein inhibitor of nNOS ( PIN ) ] was isolated from rat brain and shown to inhibit nNOS activity . ^^^ In summary , our data show that PIN , a specific inhibitor of nNOS activity , is expressed in the IMCD , a site of high nNOS expression in the kidney . ^^^ Inhibition of nNOS activity by PIN may have important implications for the regulation of NO synthesis in the IMCD of normal and remnant kidneys . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| In the mouse , two nNOS mRNAs have been identified : nNOSalpha , encoding a 155 kDa protein , and an exon 2 deletion variant , nNOSbeta , encoding a 135 kDa protein that lacks a domain where a protein inhibitor of nNOS ( PIN ) binds . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Stimulation of DLC expression resulted in an increased association of DLC / PIN with neuronal nitric oxide synthase ( nNOS ) , thereby reducing nNOS activity . ^^^ This increased nNOS activity as well as increased nNOS dimer after NGF withdrawal were inhibited by COX 2 or DLC / PIN overexpression . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| In this study we investigated the functional significance of PIN ( protein inhibitor of nNOS ) in targeting of nNOS to the sarcolemma and the association between nNOS and the dystrophin complex in normal and dystrophic muscle fibers . ^^^ The presence of PIN protein expression in muscles from mdx mice was evident despite the significant reduction in nNOS and dystrophin protein expressions in these fibers . ^^^ In muscle sections of DMD patients , the absence of nNOS protein expression was accompanied by maintained PIN expression . ^^^ These results suggest that PIN expression in muscles from mdx mice and DMD patients is controlled by factors different from those involved in the regulation of nNOS and dystrophin . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| We provide evidence that nNOS is also present in the human beta cell line CM and that the specific inhibitor of nNOS PIN is expressed in CM and INS 1 cells . ^^^ Furthermore , we investigated the influence of glucose on the activity of nNOS and the expression of PIN and are able to show that both are increased by glucose stimulation in the beta cell lines but not in the mouse fibroblastic cell line LTK . ^^^ This indicates that nNOS and PIN play a role in the specific function of beta cells , not only in rodents but also in humans . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| In the central nervous system , nNOS is activated by the N methyl D aspartate receptor ( NMDAR ) and , presumably , is inhibited by the protein inhibitor of NOS ( PIN ) . ^^^ Aging did not affect the mRNA levels of PnNOS , nNOS , NMDAR , and PIN . ^^^ Both PIN and NMDAR were detected in penile nerves of the wild type and nNOS ( / ) mouse . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Because variants of nNOS ( penile nNOS : PnNOS ) and the N methyl D aspartate receptor ( truncated NMDAR subunit 1 : NMDAR 1 T ) as well as protein inhibitor of NOS ( PIN ) have all been located in the pelvic ganglia and penile nerves , this work aims to determine whether these proteins are also present in the hypothalamus . ^^^ It was found that PnNOS , the brain type nNOS , and PIN , were expressed in the hypothalamus . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| The latter effect could be reproduced by the two endogenous inhibitors of NOS , N omega methyl l arginine and asymmetric dimethylarginine , and resulted interestingly in a reduced ability of the protein inhibitor of nNOS ( PIN ) to dissociate nNOS dimers . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Based on animal and cell studies , neurogenic ED is assumed to be caused mainly by : ( a ) an insufficient synthesis of nitric oxide ( NO ) due to a decrease in the levels of the penile neuronal nitric oxide synthase ( PnNOS ) or the impairment of its regulation by protein effectors ( NMDA receptor , protein inhibitor of nNOS : PIN ) , occurring in the neuronal bodies or nerve terminals , or ( b ) a loss of the cells themselves by apoptosis caused by the induction of inducible NOS ( iNOS ) and the production of peroxynitrite . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| A protein inhibitor of neuronal nitric oxide synthase ( nNOS ) was identified and designated as PIN . ^^^ PIN was reported to inhibit nNOS activity in cell lysates through disruption of enzyme dimerization . ^^^ However , there has been lack of direct characterization of the effect of PIN on NO production from purified nNOS . ^^^ O ( 2 ) ( ) generation from purified nNOS using electron paramagnetic resonance spin trapping techniques . nNOS was isolated by affinity chromatography and a fusion protein CBP PIN was used to probe the effect of PIN . ^^^ While the tag CBP did not affect nNOS activity , CBP PIN caused a dose dependent inhibition on both NO and L citrulline production . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| The present study investigates expression of nitric oxide synthase ( NOS ) , immediate early genes ( IEGs , c jun , and c fos ) , and low affinity nerve growth factor receptor ( LNGFR ) in adult rat spinal motoneurons in response to three conditions of axonal injury : distal axotomy , root avulsion , and root avulsion followed by a peripheral nerve ( PN ) graft implantation . ^^^ Expression of NOS was completely inhibited in all motoneurons that regenerated into the PN graft , but was not inhibited in those that did not regenerate . ^^^ The survival promoting effects of PN graft implantation ( presumably the effects of neurotrophic factors ) may be achieved by modifying certain cellular molecules such as NOS . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Each atypical biopsy was evaluated for the reason for atypia ( atrophic glands , rule out [ r / o ] adenosis , atypical not otherwise specified [ NOS ; insufficient cytologic and / or architectural atypia ] , r / o prostatic intraepithelial neoplasia [ PIN ] , inflammation , crush artifact ) and a favored diagnosis ( cancerous , benign , and undetermined ) . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| The present study examined the effects of peripheral nerve ( PN ) graft and neurotrophic factors on the expression of nitric oxide synthase ( NOS ) and the survival of Clarke ' s nucleus ( CN ) neurons at the first lumbar spinal segment ( L 1 ) 15 days after hemisection of the spinal cord at T 11 . ^^^ Transplantation of PN graft at the lesion site promoted the survival of CN neurons to 71 % and increased the expression of NOS to 70 % . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| The present study examined the effect of treatment with the NOS inhibitor N ( G ) nitro L arginine methyl ester ( L NAME ) together with peripheral nerve ( PN ) graft or brain derived neurotrophic factor ( BDNF ) on the survival of CN neurons at the L 1 level of the spinal cord following hemisection at T 11 . ^^^ Treatment with either PN graft implantation or continuous infusion of BDNF increased the survival rate of CN neurons to 70 % ; 70 % of these expressed NOS . ^^^ Combined L NAME and PN graft or L NAME and BDNF improved the rescue rate up to 94 % , but only approximately 33 % expressed NOS . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| On the basis of the combined use of dihydrorhodamine 123 and NOS inhibitors , the analysis of the amount , time course and nature of the species involved supports the view that PN generated from cNOS derived NO , while not affecting cell viability and hepatocyte monolayer development , is the main species likely responsible for the early biochemical injury commonly observed in hepatocyte cultures . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| Finally , in the case of approach c we have found that a protein inhibitor of NOS ( PIN ) is expressed in the corpora cavernosa . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NOS inhibitors and a PN scavenger were able to reduce the cellular infiltration , while displaying opposite effects on cartilage homeostasis either by enhancing or ameliorating the damage , respectively . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| One of the many players regulating NOS activity is the Protein Inhibitor of NOS , PIN . ^^^ To gain further insight into the mechanisms of NOS regulation and NO mediated cell death after nerve trauma , we examined PIN expression in a standard model of lesion induced neurodegeneration , the rat optic nerve transsection model . ^^^ These results suggest that in our model , NOS activity is not regulated by altered PIN levels , which contributes to our understanding of apoptotic mechanisms in injured neurons . . ^^^ |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P63167 |
Pubmed |
SVM Score :0.0 |
| NA |
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