| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.58386038 |
| Both ( * ) NO elevations and the association between nNOS and Hsp 90 were inhibited in rats infused with superoxide dismutase . 0.58386038^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.91434965 |
| We demonstrated an increased interaction between NOS 1 and its regulatory protein HSP 90 in post MI hearts , a potential mechanism for the higher NOS 1 activity in this setting . 0.91434965^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Pial arteriolar diameter changes in anesthetized male rats were measured during sequential applications of ACh and ADP in the absence and presence of the nonselective NOS inhibitor N ( omega ) nitro L arginine methyl ester ( L NAME ) , the neuronal NOS ( nNOS ) selective inhibitor ARR 17477 , the HSP 90 blocker 17 ( allylamino ) 17 demethoxygeldanamycin ( AAG ) , the PI3K inhibitor wortmannin ( Wort ) , or the tyrosine kinase blocker tyrphostin 47 ( T 47 ) . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| However , at 63 weeks , both eNOS and neuronal NOS ( nNOS ) were markedly upregulated in the LV of SHR , together with an upregulation of Hsp 90 . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Immunoblots of eNOS , inducible NOS ( iNOS ) , and neuronal NOS ( nNOS ) in the immunoprecipitate of HSP 90 of heat shocked cells showed that there was a sevenfold increase in eNOS and no changes in iNOS and nNOS . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| We show here that a portion of neuronal nitric oxide synthase ( nNOS ) exists as a molybdate stabilized nNOS . hsp 90 heterocomplex in the cytosolic fraction of human embryonic kidney 293 cells stably transfected with rat nNOS . ^^^ Like the GR , purified heme free apo nNOS is activated by the DE 52 retained fraction of rabbit reticulocyte lysate , which also assembles nNOS . hsp 90 heterocomplexes . ^^^ However , in contrast to the GR , heterocomplex assembly with hsp 90 is not required for increased heme binding and nNOS activation in this cell free system . ^^^ We propose that , in vivo , where access by free heme is limited , the complete hsp 90 based chaperone machinery is required for sustained opening of the heme binding cleft and nNOS activation , but in the heme containing cell free nNOS activating system transient opening of the heme binding cleft without hsp 90 is sufficient to facilitate heme binding . . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Heat shock protein 90 ( hsp 90 ) has been shown to facilitate neuronal NO synthase ( nNOS , type 1 ) activity in vivo . ^^^ But the direct effect of hsp 90 on purified nNOS has not been determined yet . ^^^ Moreover , the mechanism underlying the action of hsp 90 is not known . nNOS activity is primarily initiated and regulated by the binding of Ca ( 2+ ) / calmodulin ( CaM ) . ^^^ Therefore , we explored whether hsp 90 modulates nNOS activity by affecting CaM binding . ^^^ Recombinant rat nNOS was purified from the stably transfected cells by affinity chromatography . hsp 90 increased nNOS activity in a dose dependent manner with an EC ( 50 ) of 24 . 1+ / 6 . 4 nM . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Recent studies showed that heat shock protein 90 ( HSP 90 ) enhances nitric oxide ( NO ) synthesis from endothelial and neuronal NO synthase ( eNOS and nNOS , respectively ) . ^^^ Moreover , although our previous studies showed that the action of HSP 90 involves increased Ca ( 2+ ) / calmodulin ( Ca ( 2+ ) / CaM ) binding , quantitative measurements of the effect of HSP 90 on CaM binding to nNOS have been lacking . ^^^ HSP 90 augmented NO formation from nNOS in a dose dependent manner . ^^^ Tryptophan fluorescence quenching measurements revealed that HSP 90 markedly reduced the K ( d ) of CaM to nNOS ( 0 . 5 + / 0 . 1 nM vs . 9 . 4 + / 1 . 8 nM in the presence and absence of HSP 90 , P < 0 . 01 ) . ^^^ Ca ( 2+ ) ionophore triggered strong NO production from nNOS transfected cells , and this was significantly reduced by the HSP 90 inhibitor geldanamycin . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| It is established that neuronal NO synthase ( nNOS ) is associated with the chaperone hsp 90 , although the functional role for this interaction has not been defined . ^^^ We have discovered that inhibition of hsp 90 by radicicol or geldanamycin nearly prevents the heme mediated activation and assembly of heme deficient apo nNOS in insect cells . ^^^ The ferrous carbonyl complex of nNOS is not formed when hsp 90 is inhibited , indicating that functional heme insertion is prevented . ^^^ We propose that the hsp 90 based chaperone machinery facilitates functional heme entry into apo nNOS by the opening of the hydrophobic heme binding cleft in the protein . ^^^ At a concentration of 20 microm , geldanamycin causes a 3 fold increase in NADPH oxidation and hydrogen peroxide formation from purified nNOS , whereas the non quinone hsp 90 inhibitor radicicol had no effect . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Recently , heat shock protein 90 ( hsp 90 ) was shown to facilitate NO synthesis from eNOS and nNOS . ^^^ Therefore , we performed electron paramagnetic resonance measurements of O ( 2 ) ( . ) generation from nNOS to study the effect of hsp 90 . ^^^ In contrast to its effect on NO synthesis , hsp 90 dose dependently inhibited O ( 2 ) ( . ) generation from nNOS with an IC ( 50 ) of 658 nM . ^^^ At lower levels of L arginine where marked O ( 2 ) ( . ) generation occurred , hsp 90 caused a more dramatic enhancement of NO synthesis from nNOS as compared to that under normal L arginine . ^^^ Adding hsp 90 prevented this O ( 2 ) ( . ) production , leading to enhanced nNOS activity . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Reciprocal variations of nNOS and HSP 90 are associated with fasting in gastrointestinal tract of the piglet . ^^^ The effects of fasting on neuronal NO synthase ( nNOS ) , Heme oxygenase 2 ( HO 2 ) , and heat shock protein 90 ( HSP 90 ) was determined by immunoblotting in the stomach , duodenum , mid jejunum , distal ileum , and proximal colon of 28 day old piglets . nNOS expression was drastically reduced in all the gastrointestinal areas studied while HO 2 was not changed . ^^^ Concomitant with the nNOS decrease , elevated expressions of HSP 90 were observed in these different areas . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| The metabolism based inactivation of nNOS and the inhibition of heat shock protein 90 ( hsp 90 ) based chaperones , which are known to regulate nNOS , both lead to enhanced proteasomal degradation of nNOS . ^^^ We found that the inactive monomeric heme deficient nNOS ( apo nNOS ) is rapidly degraded in vitro , consistent with the finding that both metabolism based inactivation and inhibition of hsp 90 based chaperones cause the formation of apo nNOS and enhance its degradation in vivo . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| The proteasomal degradation of nNOS is enhanced by suicide inactivation of nNOS or by the inhibition of hsp 90 , which is a chaperone found in a native complex with nNOS . ^^^ In the current study , we have examined whether CHIP , a chaperone dependent E 3 ubiquitin protein isopeptide ligase that is known to ubiquitylate other hsp 90 chaperoned proteins , could act as an ubiquitin ligase for nNOS . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Functional evidence for interaction between HSP 90 and nitric oxide ( NO ) mediated signaling included observations that the contractile effect of geldanamycin was the following : ( 1 ) endothelium dependent , ( 2 ) abolished by Ng nitro L arginine methylester ( L NAME ; 0 . 3 mmol / L ) , and ( 3 ) non additive with the contractile effect of this NOS inhibitor ( P < 0 . 01 , n = 6 for each ) . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| To identify candidate proteins with which HSP 90 may interact to regulate metamorphosis , we noted that in mammalian cells , nitric oxide synthase ( NOS ) interacts with HSP 90 and its activity is sensitive to drugs that inhibit HSP 90 function . ^^^ Inhibition of NOS activity in these ascidian larvae with L NAME increased the frequency of metamorphosis , consistent with a putative interaction of NOS and HSP 90 . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Nitric oxide synthase ( NOS ) activity in some mammalian cells , including neurons , depends on the molecular chaperone heat shock protein 90 ( HSP 90 ) ; this may be so in echinoid larvae as well . ^^^ Pluteus larvae containing juvenile rudiments were treated with either radicicol L or D nitroarginine methyl ester ( L NAME and D NAME ) , or IH [ 1 , 2 , 4 ] oxadiazolo [ 4 , 3 a ] quinoxalin 1 one ( ODQ ) , inhibitors of HSP 90 , NOS , and sGC , respectively . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Propranolol decreased NOS activity by 47 % and eNOS and Hsp 90 expression by 75 % and 72 % , respectively . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Estrogen receptors ( ER alpha and ER beta ) , NOS , heat shock proteins 70 and 90 ( HSP 70 and HSP 90 ) , caveolin 1 , 2 , and 3 , calmodulin , NOS activity , and cGMP were analyzed in a lysate of platelets from gonadally intact and ovariectomized female pigs . ^^^ Expression of ER beta and ER alpha receptors , endothelial NOS ( eNOS ) , HSP 70 , and HSP 90 increased with ovariectomy . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| This avenue of investigation was based on recent reports suggesting that hsp 90 modulates NOS production of * NO and O 2 . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| The response of control pulmonary arteries to ATP was attenuated by NG nitro l arginine methyl ester ( l NAME ) , a NOS antagonist , and geldanamycin , an inhibitor of HSP 90 eNOS interaction . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NOS 3 , HSP 90 , and caveolin 1 levels were similar in hypoxic and control PAs . ^^^ After 10 days of hypoxia , NO function is impaired in PAs despite preserved levels of NOS 3 , HSP 90 , and caveolin 1 . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Tissue levels of connexins 40 , 43 ( major components of gap junction ) , inducible NOS ( iNOS ) , endothelial NOS ( eNOS ) and eNOS regulator proteins such as calmodulin , heat shock protein 90 ( hsp 90 ) and caveolin 1 were also examined using Western blot . 2 . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| Both strains exhibited the same level of NOS 3 and hsp 90 protein expression . ^^^ However , hsp 90 association with NOS 3 in BN / Mcw hearts was increased twofold compared with SS / Mcw hearts . ^^^ These findings indicate that hsp 90 with NOS 3 increases . ^^^ We conclude increased association of hsp 90 with NOS 3 is a major mechanism by which BN / Mcw hearts are more resistant to ischemia than SS / Mcw hearts . . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| In the rostral ventrolateral medulla , the 70 kDa heat shock protein ( HSP 70 ) , but not HSP 90 , confers neuroprotection against fatal endotoxemia via augmentation of nitric oxide synthase 1 ( NOS 1 ) / protein kinase G signaling pathway and inhibition of NOS II / peroxynitrite cascade . ^^^ We conclude that whereas HSP 90 is ineffective , de novo synthesis of HSP 70 in the RVLM may confer neuroprotection during fatal endotoxemia by preventing cardiovascular depression via enhancing the sympathoexcitatory NOS I / PKG signaling pathway and inhibiting the sympathoinhibitory NOS II / peroxynitrite cascade in the RVLM . . ^^^ |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29475 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
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