| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Antibodies against the main proteins in hepatic gap junctions connexin 26 and connexin 32 have been used in conjunction with high resolution patch clamp techniques to investigate whether a relation exists between connexin type and conductance of single gap junctional channels . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The hepatocyte specific phenotype of murine liver cells correlates with high expression of connexin 32 and connexin 26 but very low expression of connexin 43 . ^^^ Several clones of mouse embryonic hepatocytes immortalized in serum free MX 83 medium by infection with recombinant retrovirus expressed transcripts for connexin 32 , connexin 26 , albumin , alpha fetoprotein , tyrosine aminotransferase , as well as aldolase A and B , at more than half of the levels found in primary mouse hepatocytes . ^^^ We found that expression of connexin 32 and connexin 26 mRNAs as well as transcripts of other liver specific proteins was reversibly decreased in serum containing medium , whereas the expression level of connexin 43 transcripts was increased in serum containing DMEM compared to serum free MX 83 medium . ^^^ The expression levels of connexin 26 , connexin 32 , or connexin 43 mRNAs were altered by the addition of fetal calf serum or arginine or by the absence of hydrocortisone in MX 83 medium , all of which contributed to the shift in phenotype . ^^^ Furthermore several dedifferentiated cell lines derived from rat or mouse liver and cultivated in serum containing medium were found to express little connexin 32 or connexin 26 mRNA but relatively high levels of connexin 43 mRNA . . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| To gain insight into the function of gap junctions ' connexin 43 , connexin 32 and connexin 26 in a neural structure that retains neuronal turnover capacities throughout adulthood , the expression of these molecules has been investigated in the developing and adult olfactory system by immunocytochemical and biochemical methods . ^^^ Northern blot analysis revealed the presence of mRNA of connexin 32 and connexin 26 in the adult olfactory system . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Northern blot analyses revealed that mRNA encoding connexin 26 was 2 . 8 times more abundant in the periportal than in the pericentral cells , while connexin 32 mRNA was equally distributed . ^^^ The ratio of connexin 26 to connexin 32 mRNA in the portal fraction was about 0 . 085 , and in the central fraction about 0 . 038 . ^^^ Connexin 26 mRNA was transcribed , however , at a faster rate than connexin 32 mRNA by nuclei isolated from both cellular fractions . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Northern analysis showed that connexin 43 was the only gap junction protein message expressed in these cell lines ; connexin 32 and connexin 26 were not expressed . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| We found that a rat liver epithelial cell line ( IAR 20 ) expresses connexin 43 , the major cardiac gap junction protein , but not connexin 26 or connexin 32 , major liver gap junction proteins . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Two gap junction proteins , connexin 26 and connexin 43 , were identified between leptomeningeal cells using immunocytochemical methods ; Northern blot analyses of RNA isolated from cultured leptomeningeal cells showed specific hybridization to cDNAs encoding connexins 26 and 43 , but not to a cDNA encoding connexin 32 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Since gap junctions are formed by a family of related proteins ( connexins ) whose unique domains may confer physiological regulatory properties , we tested total RNA from these cultures by Northern blot analysis for expression of the currently available , characterized , and cloned mammalian gap junction proteins : connexin 26 , connexin 32 , and connexin 43 . ^^^ When high stringency Northern blots were used , messenger RNAs for connexin 32 or connexin 26 were not detected in any of the vascular wall cells but were expressed in several cell lines of epithelial origin . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| By probing somatic cell hybrid DNA on Southern filters with rat or human cDNAs or human genomic fragments , we have mapped four functioning gap junction genes , ( alpha 1 , beta 1 , beta 2 , and alpha 3 ) , to different sites on human chromosomes : GJA 1 ( connexin 43 ) to 6p21 . 1 q24 . 1 ; GJB 1 ( connexin 32 ) to Xcen q 22 ; GJB 2 ( connexin 26 ) to 13 ; and GJA 3 ( connexin 46 ) also to 13 , probably near GJB 2 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Premature stop codons were inserted , resulting in proteins corresponding in length to the mammalian isoforms connexin 32 and connexin 26 , which are expressed primarily in liver . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Comparative immunocytochemical labeling utilizing different antibodies specific for liver connexin 32 and connexin 26 and antibodies to peptides corresponding to carboxy terminal sequences of the heart gap junction protein ( connexin 43 ) indicates that the predominant gap junction protein in astrocytes is connexin 43 . ^^^ Cultured astrocytes expressed connexin 43 mRNA and did not contain detectable levels of the mRNAs encoding connexin 32 or connexin 26 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Between 3 and 6 weeks after birth , connexin 26 largely disappeared from immature brain ; this time course corresponded to the increased expression of connexin 32 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Normal liver expressed high connexin 32 , low connexin 26 , and barely detectable connexin 43 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In WB F 344 rat liver epithelial cells , which were highly coupled and expressed high levels of connexin 43 and no detectable connexin 32 or connexin 26 , dexamethasone had no effect on coupling or connexin expression . ^^^ These results indicate that dye coupling and the expression of connexin 32 and connexin 26 , but not connexin 43 , were upregulated by glucocorticoids in a cell specific manner . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Neither connexin 32 nor 26 was revealed , except for connexin 26 in the tonsil epithelium . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Human HeLa cells transfected with mouse DNA coding for connexin 26 ( Cx 26 ) or connexin 32 ( Cx 32 ) were used to examine the properties of heterotypic Cx 26 Cx32 gap junction channels . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In the adult rat liver , the gap junction protein connexin 32 ( Cx 32 ) is evenly distributed in hepatocytes within the liver lobules , while connexin 26 ( Cx 26 ) is preferentially localized in hepatocytes in periportal zones . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| We examined the immunofluorescent ( IF ) localization and mRNA and protein levels of the two hepatocyte gap junction proteins connexin 32 and connexin 26 , after hepatic injury induced by common bile duct ligation ( CBDL ) in the rat . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The gap junction proteins beta 1 connexin ( connexin 32 ) and beta 2 connexin ( connexin 26 ) can form heteromeric hemichannels . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| To determine which connexins were likely to be expressed in the sinus node , atrioventricular ( AV ) node and atrial and ventricular myocardium , messenger ribonucleic acids ( RNAs ) from each of these sites were hybridized with probes for connexin 26 , connexin 31 , connexin 32 , connexin 37 , connexin 40 , connexin 43 , connexin 45 , connexin 46 and connexin 50 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The topological structure of connexin 26 and its distribution compared to connexin 32 in hepatic gap junctions . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Western blots of Percoll gradient purified Leydig cells using specific antibodies indicate that connexin 43 is indeed expressed in these cells , whereas connexin 26 and connexin 32 are not . . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The identification of a family of GJ proteins , termed connexins , prompted this study of connexin 32 ( Cx 32 ) , connexin 26 ( Cx 26 ) and connexin 43 ( Cx 43 ) expression during rat hepatocarcinogenesis . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The mRNA and protein expression of alpha 1 ( connexin 43 ) , beta 1 ( connexin 32 ) , and beta 2 ( connexin 26 ) gap junction genes were examined in the regenerating rat liver after 70 % partial hepatectomy ( PH ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| By use of site specific antibodies against synthetic oligopeptides , we examined the localizations of the gap junction proteins connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) in rat and guinea pig liver . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 , but not connexin 26 , is phosphorylated by both protein kinase A and protein kinase C in serine residues and the sites of phosphorylation by both kinases remain in the major 26 kDa fragment resulting from calpain proteolysis . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Indirect immunofluorescent staining with anti gap junction protein ( connexin ) antibodies revealed that C 10 cells expressed gap junctions comprised of connexin 43 , but not connexin 32 or connexin 26 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In the adult rat hepatocyte , gap junction proteins consist of connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Although we recently reported our success in inducing and maintaining the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) in adult rat hepatocytes cultured in serum free L 15 medium supplemented with epidermal growth factor , dimethylsulfoxide ( DMSO ) and glucagon , the mechanisms by which DMSO induces gap junctions are still not clear . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Heterotypic pairings of connexin 30 with connexin 26 and connexin 32 produced channels with highly asymmetric and rectifying voltage gating , respectively . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Both experimental and control rat thyroid tissues produced gap junction proteins connexin 43 , connexin 32 , and connexin 26 . ^^^ Both nondiseased and diseased thyroid tissue expressed messenger RNA ( mRNA ) for the three connexins , but the diseased tissue had reduced levels of mRNA for connexin 43 ( 45 % ) , and to a lesser extent , connexin 26 ( 25 % ) and connexin 32 ( 20 % ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In connexin 32 defective liver , the amount of connexin 26 protein expressed was found to be lower than in wild type liver , and the total area of gap junction plaques was approximately 1000 fold smaller than in wild type liver . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In adult rat teeth , connexin 26 and 43 were expressed in the odontoblastic layer at low and high levels , respectively , while connexin 32 was absent from odontoblasts . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Antibodies that labelled astrocytic gap junctions exhibited no cross reaction with connexin 43 or connexin 32 , as demonstrated by western blotting , but recognized liver connexin 26 as well as several brain proteins , including an approximately 32000 mol . wt protein that did not correspond to connexin 32 and a 26000 mol . wt protein that co migrated with liver connexin 26 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Northern , Western , and immunohistochemical analyses indicated that all four cell lines expressed comparable levels of connexin 43 ( Cx 43 ) , but not connexin 32 or connexin 26 , and formed Cx 43 containing gap junction plaques at cell cell interfaces . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Whereas connexin 26 mRNA declined approximately 40 % in cultured cells , connexin 32 message decreased rapidly and was not detectable on day 1 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Liver gap junctions ( whose connexons contain mainly connexin 32 ) and recombinant connexons constructed of connexin 26 expressed by baculovirus infected insect cells exhibited weaker binding of TA calmodulin with variable Ca2+ dependence . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 aequorin and connexin 43 aequorin retained chemiluminescent activity whereas that of connexin 26 aequorin was negligible . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The data define different size cut off limits for permeability through homomeric connexin 32 channels and through heteromeric connexin 32 / connexin 26 channels . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The results show that all GnRH neurons contain connexin 32 immunoreactive puncta at their perikarya and , occasionally , at their axon terminals in the median eminence while connexin 26 and 43 immunoreactivity was absent in GnRH neurons . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| To determine whether junctional communication between pancreatic acinar cells contributes to their secretory function in vivo , we have compared wild type mice , which express the gap junctional proteins connexin 32 ( Cx 32 ) and connexin 26 , to mice deficient for the Cx 32 gene . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Using a modified primary cell culture of rat hepatocytes , in which both connexin 32 and connexin 26 were expressed in a comparable degree , the molecular composition of connexin subtypes in a gap junction channel ( i . e . , homomeric or heteromeric ) was studied . ^^^ Similarly , either one of the antibodies raised against connexin 32 or connexin 26 completely inhibited dye coupling activity among hepatocytes . ^^^ In addition , we studied the dye coupling properties at different extracellular pHs in two primary rat hepatocytes : one in which connexin 26 was induced , and the other which expresses connexin 32 as a major gap junction channel protein . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Cytoplasmic levels of free calcium surrounding the ERGIC / Golgi reported by connexin 43 chimera ( approximately 420 nM ) were twice those measured by connexin 32 chimera ( approximately 200 nM ) ; both chimerae measured calcium levels substantially higher than those reported by a connexin 26 chimera ( approximately 130 nM ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Modulation by aminosulfonates is specific for heteromeric connexin channels containing connexin 26 ; it does not occur significantly for homomeric connexin 32 channels . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) protein levels were assayed by Western immunoblotting and developed according to age and exposure to glucocorticoids . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Intracellular membranes were shown to contain low amounts of connexin 26 relative to connexin 32 in contrast to the equal connexin ratios detected in lateral plasma membranes and gap junctions . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Different changes in expression and function of connexin 26 and connexin 32 during DNA synthesis and redifferentiation in primary rat hepatocytes using a DMSO culture system . ^^^ In the present study , we determined in detail the changes of liver gap junctions , connexin 26 ( Cx 26 ) , and connexin 32 ( Cx 32 ) , during DNA synthesis and redifferentiation of hepatocytes in vitro . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical studies showed that rat , hamster , mouse , and human oviducts contained connexin 26 and connexin 43 but not connexin 32 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The major connexins of liver ( hepatocytes ) are connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| We examined the expression pattern of two major gap junction proteins , connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) , in rat mammary glands during pregnancy and lactation . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Reduction of malignant phenotype of HEPG 2 cell is associated with the expression of connexin 26 but not connexin 32 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Gap junction channels in the rodent liver are composed of connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) proteins . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Our study identified two gap junctional proteins , connexin 26 and connexin 32 , in rat gastric glands by RT PCR , Western blot analysis , and immunofluorescence . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Localization of connexin 26 and connexin 32 in putative CO ( 2 ) chemosensitive brainstem regions in rat . ^^^ Here , we used immunoblot and immunohistochemical analyses to investigate the presence , distribution , and cellular localization of the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) in putative CO ( 2 ) chemosensitive brainstem regions in both neonatal and adult rats . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Differential expression of connexin 26 and connexin 32 in the pre Btzinger complex of neonatal and adult rat . ^^^ We found that both connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) were expressed in pre BtC neurons of neonatal and adult rats ; however , the relative amounts and their distribution varied by age . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| The fully open state of heterotypic gap junction channels formed by pairing cells expressing connexin 32 ( Cx 32 ) with those expressing connexin 26 ( Cx 26 ) rectifies in a way that can not be predicted from the current voltage ( 1 5 ) relation of either homotypic channel . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In both normal and neoplastic adrenal tissues , only alpha 1 connexin 43 could be detected , whereas beta 1 connexin 32 and beta 2 connexin 26 were not found . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND / AIMS : It has previously been shown in rat liver that the gap junctional proteins connexin 32 and connexin 26 are downregulated when murine hepatocytes are in the S phase of the cell cycle . ^^^ CONCLUSIONS : Loss of connexin 32 protein and / or diminished expression of connexin 26 did not promote G0 / 1 S transition of hepatocytes in two thirds hepatectomized mouse livers . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Acute phase response and circadian expression of connexin 26 are not altered in connexin 32 deficient mouse liver . ^^^ In mouse hepatocytes , the gap junctional proteins connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) are expressed in the same gap junctional plaque . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 and 26 protein expression , as assessed by immunoblotting , was similar in nafenopin treated hepatocytes compared to controls , with the exception that in a 10 h culture with nafenopin , the level of connexin 26 was elevated compared to controls . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Chronic ( 18 h ) exposure of cultured hippocampal slices to the type A GABA receptor blocker , bicuculline methiodide ( BMI ) 10 micro m increased the levels of connexin 43 ( Cx 43 ) and connexin 32 ( Cx 32 ) mRNAs , but not connexin 26 and connexin 36 , as demonstrated by RNase protection assays . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| We further show that connexin 32 and connexin 26 expression and gap junction plaque formation increase over time in culture concomitant with an increase in GJIC between adjoining primary mouse hepatocytes . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Recent work from our laboratory has demonstrated that the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) are expressed in neurons in putative CO 2 chemosensitive brainstem regions in both neonatal and adult rats . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Open pore block of connexin 26 and connexin 32 hemichannels by neutral , acidic and basic glycoconjugates . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation experiments revealed that connexin 33 physically interacted with connexin 43 , mainly with the phosphorylated P 1 isoform of connexin 43 but not with connexin 26 and connexin 32 , two other connexins expressed in the tubular compartment . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In the immunohistochemical study , significant immunoreactivity of connexin 26 and connexin 32 was also observed . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| These cells also failed to maintain expression of the gap junction proteins connexin 26 and connexin 32 , leading to the loss of gap junctions . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| We examined protein components of the gap junctions between acinar cells of the parotid , sublingual and submandibular glands of the rat , using type specific antibodies directed against major gap junction proteins , connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| After immediate fixation in formalin and ethanol , the samples were immunohistochemically stained for E cadherin ( HECD 1 ) , Bcl 2 , Ki 67 ( MIB 1 ) , connexin 26 and connexin 32 , beta catenin and gamma catenin . ^^^ Connexin 26 showed similar localization to that of connexin 32 but the staining was much more intense . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning of two guinea pig liver gap junction proteins , connexin 32 and connexin 26 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Phenobarbital did not alter connexin 32 and connexin 26 expression , but increased hepatic Cyp2b1 expression and the liver weight : body weight ratio in both strains . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| In pancreatic acini , the connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) proteins form functional channels that coordinate the secretion of digestive enzymes . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Isoelectric points and post translational modifications of connexin 26 and connexin 32 . ^^^ The isoelectric points of the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) were determined by isoelectric focusing in free fluids . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Gap junctions between murine hepatocytes are composed of two subunit proteins , connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Valve ICs did not express connexin 32 and 40 ; however , connexin 26 and 43 were equally expressed by a low percentage of ICs , demonstrating cell surface and cytoplasmic expression , and connexin 45 was weakly expressed . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Furthermore , CMTX mutants selectively acted as dominant inhibitors of intercellular communication by interfering with the channel forming ability of connexin 26 but not with that of connexin 40 . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Mutations in these genes cause autosomal recessive ( GJB 2 and GJB 3 ) , autosomal dominant ( GJB 2 , GJB 3 , and GJB 6 ) or 10 linked ( GJB 1 ) hearing impairment , both syndromic ( GJB 2 , keratoderma ; GJB 3 erythrokeratodermia variabilis ; and GJB 1 , peripheral neuropathy ) , and non syndromic ( GJB 2 , GJB 3 , and GJB 6 ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Mutations in four members of the connexin gene family have been shown to underlie distinct genetic forms of deafness , including GJB 2 [ connexin 26 ( Cx 26 ) ] , GJB 3 ( Cx 31 ) , GJB 6 ( Cx 30 ) and GJB 1 ( Cx 32 ) . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Mutations in GJB 1 , GJB 2 , GJB 3 and GJB 6 are involved in hearing impairment . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| Several connexin genes ( GJB 1 , GJB 2 , GJB 3 , GJB 6 and GJA 1 ) have been found mutated in patients with non syndromic and / or syndromic deafness indicating an important role of these proteins in the auditory system . ^^^ |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08034 and P29033 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Antibodies against the main proteins in hepatic gap junctions connexin 26 and connexin 32 have been used in conjunction with high resolution patch clamp techniques to investigate whether a relation exists between connexin type and conductance of single gap junctional channels . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Northern analysis showed that connexin 43 was the only gap junction protein message expressed in these cell lines ; connexin 32 and connexin 26 were not expressed . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| We found that a rat liver epithelial cell line ( IAR 20 ) expresses connexin 43 , the major cardiac gap junction protein , but not connexin 26 or connexin 32 , major liver gap junction proteins . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Two gap junction proteins , connexin 26 and connexin 43 , were identified between leptomeningeal cells using immunocytochemical methods ; Northern blot analyses of RNA isolated from cultured leptomeningeal cells showed specific hybridization to cDNAs encoding connexins 26 and 43 , but not to a cDNA encoding connexin 32 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The hepatocyte specific phenotype of murine liver cells correlates with high expression of connexin 32 and connexin 26 but very low expression of connexin 43 . ^^^ Several clones of mouse embryonic hepatocytes immortalized in serum free MX 83 medium by infection with recombinant retrovirus expressed transcripts for connexin 32 , connexin 26 , albumin , alpha fetoprotein , tyrosine aminotransferase , as well as aldolase A and B , at more than half of the levels found in primary mouse hepatocytes . ^^^ We found that expression of connexin 32 and connexin 26 mRNAs as well as transcripts of other liver specific proteins was reversibly decreased in serum containing medium , whereas the expression level of connexin 43 transcripts was increased in serum containing DMEM compared to serum free MX 83 medium . ^^^ The expression levels of connexin 26 , connexin 32 , or connexin 43 mRNAs were altered by the addition of fetal calf serum or arginine or by the absence of hydrocortisone in MX 83 medium , all of which contributed to the shift in phenotype . ^^^ Furthermore several dedifferentiated cell lines derived from rat or mouse liver and cultivated in serum containing medium were found to express little connexin 32 or connexin 26 mRNA but relatively high levels of connexin 43 mRNA . . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| To gain insight into the function of gap junctions ' connexin 43 , connexin 32 and connexin 26 in a neural structure that retains neuronal turnover capacities throughout adulthood , the expression of these molecules has been investigated in the developing and adult olfactory system by immunocytochemical and biochemical methods . ^^^ Northern blot analysis revealed the presence of mRNA of connexin 32 and connexin 26 in the adult olfactory system . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Northern blot analyses revealed that mRNA encoding connexin 26 was 2 . 8 times more abundant in the periportal than in the pericentral cells , while connexin 32 mRNA was equally distributed . ^^^ The ratio of connexin 26 to connexin 32 mRNA in the portal fraction was about 0 . 085 , and in the central fraction about 0 . 038 . ^^^ Connexin 26 mRNA was transcribed , however , at a faster rate than connexin 32 mRNA by nuclei isolated from both cellular fractions . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Neither connexin 32 nor 26 was revealed , except for connexin 26 in the tonsil epithelium . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Human HeLa cells transfected with mouse DNA coding for connexin 26 ( Cx 26 ) or connexin 32 ( Cx 32 ) were used to examine the properties of heterotypic Cx 26 Cx32 gap junction channels . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In the adult rat liver , the gap junction protein connexin 32 ( Cx 32 ) is evenly distributed in hepatocytes within the liver lobules , while connexin 26 ( Cx 26 ) is preferentially localized in hepatocytes in periportal zones . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Since gap junctions are formed by a family of related proteins ( connexins ) whose unique domains may confer physiological regulatory properties , we tested total RNA from these cultures by Northern blot analysis for expression of the currently available , characterized , and cloned mammalian gap junction proteins : connexin 26 , connexin 32 , and connexin 43 . ^^^ When high stringency Northern blots were used , messenger RNAs for connexin 32 or connexin 26 were not detected in any of the vascular wall cells but were expressed in several cell lines of epithelial origin . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| By probing somatic cell hybrid DNA on Southern filters with rat or human cDNAs or human genomic fragments , we have mapped four functioning gap junction genes , ( alpha 1 , beta 1 , beta 2 , and alpha 3 ) , to different sites on human chromosomes : GJA 1 ( connexin 43 ) to 6p21 . 1 q24 . 1 ; GJB 1 ( connexin 32 ) to Xcen q 22 ; GJB 2 ( connexin 26 ) to 13 ; and GJA 3 ( connexin 46 ) also to 13 , probably near GJB 2 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Premature stop codons were inserted , resulting in proteins corresponding in length to the mammalian isoforms connexin 32 and connexin 26 , which are expressed primarily in liver . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Comparative immunocytochemical labeling utilizing different antibodies specific for liver connexin 32 and connexin 26 and antibodies to peptides corresponding to carboxy terminal sequences of the heart gap junction protein ( connexin 43 ) indicates that the predominant gap junction protein in astrocytes is connexin 43 . ^^^ Cultured astrocytes expressed connexin 43 mRNA and did not contain detectable levels of the mRNAs encoding connexin 32 or connexin 26 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Between 3 and 6 weeks after birth , connexin 26 largely disappeared from immature brain ; this time course corresponded to the increased expression of connexin 32 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Normal liver expressed high connexin 32 , low connexin 26 , and barely detectable connexin 43 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In WB F 344 rat liver epithelial cells , which were highly coupled and expressed high levels of connexin 43 and no detectable connexin 32 or connexin 26 , dexamethasone had no effect on coupling or connexin expression . ^^^ These results indicate that dye coupling and the expression of connexin 32 and connexin 26 , but not connexin 43 , were upregulated by glucocorticoids in a cell specific manner . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The mRNA and protein expression of alpha 1 ( connexin 43 ) , beta 1 ( connexin 32 ) , and beta 2 ( connexin 26 ) gap junction genes were examined in the regenerating rat liver after 70 % partial hepatectomy ( PH ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| By use of site specific antibodies against synthetic oligopeptides , we examined the localizations of the gap junction proteins connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) in rat and guinea pig liver . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 , but not connexin 26 , is phosphorylated by both protein kinase A and protein kinase C in serine residues and the sites of phosphorylation by both kinases remain in the major 26 kDa fragment resulting from calpain proteolysis . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| We examined the immunofluorescent ( IF ) localization and mRNA and protein levels of the two hepatocyte gap junction proteins connexin 32 and connexin 26 , after hepatic injury induced by common bile duct ligation ( CBDL ) in the rat . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The gap junction proteins beta 1 connexin ( connexin 32 ) and beta 2 connexin ( connexin 26 ) can form heteromeric hemichannels . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| To determine which connexins were likely to be expressed in the sinus node , atrioventricular ( AV ) node and atrial and ventricular myocardium , messenger ribonucleic acids ( RNAs ) from each of these sites were hybridized with probes for connexin 26 , connexin 31 , connexin 32 , connexin 37 , connexin 40 , connexin 43 , connexin 45 , connexin 46 and connexin 50 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Furthermore , CMTX mutants selectively acted as dominant inhibitors of intercellular communication by interfering with the channel forming ability of connexin 26 but not with that of connexin 40 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The topological structure of connexin 26 and its distribution compared to connexin 32 in hepatic gap junctions . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Western blots of Percoll gradient purified Leydig cells using specific antibodies indicate that connexin 43 is indeed expressed in these cells , whereas connexin 26 and connexin 32 are not . . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The identification of a family of GJ proteins , termed connexins , prompted this study of connexin 32 ( Cx 32 ) , connexin 26 ( Cx 26 ) and connexin 43 ( Cx 43 ) expression during rat hepatocarcinogenesis . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Indirect immunofluorescent staining with anti gap junction protein ( connexin ) antibodies revealed that C 10 cells expressed gap junctions comprised of connexin 43 , but not connexin 32 or connexin 26 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In the adult rat hepatocyte , gap junction proteins consist of connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Although we recently reported our success in inducing and maintaining the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) in adult rat hepatocytes cultured in serum free L 15 medium supplemented with epidermal growth factor , dimethylsulfoxide ( DMSO ) and glucagon , the mechanisms by which DMSO induces gap junctions are still not clear . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Heterotypic pairings of connexin 30 with connexin 26 and connexin 32 produced channels with highly asymmetric and rectifying voltage gating , respectively . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Both experimental and control rat thyroid tissues produced gap junction proteins connexin 43 , connexin 32 , and connexin 26 . ^^^ Both nondiseased and diseased thyroid tissue expressed messenger RNA ( mRNA ) for the three connexins , but the diseased tissue had reduced levels of mRNA for connexin 43 ( 45 % ) , and to a lesser extent , connexin 26 ( 25 % ) and connexin 32 ( 20 % ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In connexin 32 defective liver , the amount of connexin 26 protein expressed was found to be lower than in wild type liver , and the total area of gap junction plaques was approximately 1000 fold smaller than in wild type liver . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In adult rat teeth , connexin 26 and 43 were expressed in the odontoblastic layer at low and high levels , respectively , while connexin 32 was absent from odontoblasts . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Antibodies that labelled astrocytic gap junctions exhibited no cross reaction with connexin 43 or connexin 32 , as demonstrated by western blotting , but recognized liver connexin 26 as well as several brain proteins , including an approximately 32000 mol . wt protein that did not correspond to connexin 32 and a 26000 mol . wt protein that co migrated with liver connexin 26 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Northern , Western , and immunohistochemical analyses indicated that all four cell lines expressed comparable levels of connexin 43 ( Cx 43 ) , but not connexin 32 or connexin 26 , and formed Cx 43 containing gap junction plaques at cell cell interfaces . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Whereas connexin 26 mRNA declined approximately 40 % in cultured cells , connexin 32 message decreased rapidly and was not detectable on day 1 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Liver gap junctions ( whose connexons contain mainly connexin 32 ) and recombinant connexons constructed of connexin 26 expressed by baculovirus infected insect cells exhibited weaker binding of TA calmodulin with variable Ca2+ dependence . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Different changes in expression and function of connexin 26 and connexin 32 during DNA synthesis and redifferentiation in primary rat hepatocytes using a DMSO culture system . ^^^ In the present study , we determined in detail the changes of liver gap junctions , connexin 26 ( Cx 26 ) , and connexin 32 ( Cx 32 ) , during DNA synthesis and redifferentiation of hepatocytes in vitro . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical studies showed that rat , hamster , mouse , and human oviducts contained connexin 26 and connexin 43 but not connexin 32 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 aequorin and connexin 43 aequorin retained chemiluminescent activity whereas that of connexin 26 aequorin was negligible . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The data define different size cut off limits for permeability through homomeric connexin 32 channels and through heteromeric connexin 32 / connexin 26 channels . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The results show that all GnRH neurons contain connexin 32 immunoreactive puncta at their perikarya and , occasionally , at their axon terminals in the median eminence while connexin 26 and 43 immunoreactivity was absent in GnRH neurons . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| To determine whether junctional communication between pancreatic acinar cells contributes to their secretory function in vivo , we have compared wild type mice , which express the gap junctional proteins connexin 32 ( Cx 32 ) and connexin 26 , to mice deficient for the Cx 32 gene . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Using a modified primary cell culture of rat hepatocytes , in which both connexin 32 and connexin 26 were expressed in a comparable degree , the molecular composition of connexin subtypes in a gap junction channel ( i . e . , homomeric or heteromeric ) was studied . ^^^ Similarly , either one of the antibodies raised against connexin 32 or connexin 26 completely inhibited dye coupling activity among hepatocytes . ^^^ In addition , we studied the dye coupling properties at different extracellular pHs in two primary rat hepatocytes : one in which connexin 26 was induced , and the other which expresses connexin 32 as a major gap junction channel protein . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Cytoplasmic levels of free calcium surrounding the ERGIC / Golgi reported by connexin 43 chimera ( approximately 420 nM ) were twice those measured by connexin 32 chimera ( approximately 200 nM ) ; both chimerae measured calcium levels substantially higher than those reported by a connexin 26 chimera ( approximately 130 nM ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Modulation by aminosulfonates is specific for heteromeric connexin channels containing connexin 26 ; it does not occur significantly for homomeric connexin 32 channels . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) protein levels were assayed by Western immunoblotting and developed according to age and exposure to glucocorticoids . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Intracellular membranes were shown to contain low amounts of connexin 26 relative to connexin 32 in contrast to the equal connexin ratios detected in lateral plasma membranes and gap junctions . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The fully open state of heterotypic gap junction channels formed by pairing cells expressing connexin 32 ( Cx 32 ) with those expressing connexin 26 ( Cx 26 ) rectifies in a way that can not be predicted from the current voltage ( 1 5 ) relation of either homotypic channel . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In both normal and neoplastic adrenal tissues , only alpha 1 connexin 43 could be detected , whereas beta 1 connexin 32 and beta 2 connexin 26 were not found . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND / AIMS : It has previously been shown in rat liver that the gap junctional proteins connexin 32 and connexin 26 are downregulated when murine hepatocytes are in the S phase of the cell cycle . ^^^ CONCLUSIONS : Loss of connexin 32 protein and / or diminished expression of connexin 26 did not promote G0 / 1 S transition of hepatocytes in two thirds hepatectomized mouse livers . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| The major connexins of liver ( hepatocytes ) are connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| We examined the expression pattern of two major gap junction proteins , connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) , in rat mammary glands during pregnancy and lactation . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Reduction of malignant phenotype of HEPG 2 cell is associated with the expression of connexin 26 but not connexin 32 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Gap junction channels in the rodent liver are composed of connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) proteins . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Our study identified two gap junctional proteins , connexin 26 and connexin 32 , in rat gastric glands by RT PCR , Western blot analysis , and immunofluorescence . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Localization of connexin 26 and connexin 32 in putative CO ( 2 ) chemosensitive brainstem regions in rat . ^^^ Here , we used immunoblot and immunohistochemical analyses to investigate the presence , distribution , and cellular localization of the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) in putative CO ( 2 ) chemosensitive brainstem regions in both neonatal and adult rats . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Mutations in four members of the connexin gene family have been shown to underlie distinct genetic forms of deafness , including GJB 2 [ connexin 26 ( Cx 26 ) ] , GJB 3 ( Cx 31 ) , GJB 6 ( Cx 30 ) and GJB 1 ( Cx 32 ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Differential expression of connexin 26 and connexin 32 in the pre Btzinger complex of neonatal and adult rat . ^^^ We found that both connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) were expressed in pre BtC neurons of neonatal and adult rats ; however , the relative amounts and their distribution varied by age . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| We examined protein components of the gap junctions between acinar cells of the parotid , sublingual and submandibular glands of the rat , using type specific antibodies directed against major gap junction proteins , connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| After immediate fixation in formalin and ethanol , the samples were immunohistochemically stained for E cadherin ( HECD 1 ) , Bcl 2 , Ki 67 ( MIB 1 ) , connexin 26 and connexin 32 , beta catenin and gamma catenin . ^^^ Connexin 26 showed similar localization to that of connexin 32 but the staining was much more intense . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Acute phase response and circadian expression of connexin 26 are not altered in connexin 32 deficient mouse liver . ^^^ In mouse hepatocytes , the gap junctional proteins connexin 32 ( Cx 32 ) and connexin 26 ( Cx 26 ) are expressed in the same gap junctional plaque . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Connexin 32 and 26 protein expression , as assessed by immunoblotting , was similar in nafenopin treated hepatocytes compared to controls , with the exception that in a 10 h culture with nafenopin , the level of connexin 26 was elevated compared to controls . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning of two guinea pig liver gap junction proteins , connexin 32 and connexin 26 . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Phenobarbital did not alter connexin 32 and connexin 26 expression , but increased hepatic Cyp2b1 expression and the liver weight : body weight ratio in both strains . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In pancreatic acini , the connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) proteins form functional channels that coordinate the secretion of digestive enzymes . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Chronic ( 18 h ) exposure of cultured hippocampal slices to the type A GABA receptor blocker , bicuculline methiodide ( BMI ) 10 micro m increased the levels of connexin 43 ( Cx 43 ) and connexin 32 ( Cx 32 ) mRNAs , but not connexin 26 and connexin 36 , as demonstrated by RNase protection assays . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| We further show that connexin 32 and connexin 26 expression and gap junction plaque formation increase over time in culture concomitant with an increase in GJIC between adjoining primary mouse hepatocytes . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Recent work from our laboratory has demonstrated that the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) are expressed in neurons in putative CO 2 chemosensitive brainstem regions in both neonatal and adult rats . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Open pore block of connexin 26 and connexin 32 hemichannels by neutral , acidic and basic glycoconjugates . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation experiments revealed that connexin 33 physically interacted with connexin 43 , mainly with the phosphorylated P 1 isoform of connexin 43 but not with connexin 26 and connexin 32 , two other connexins expressed in the tubular compartment . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| In the immunohistochemical study , significant immunoreactivity of connexin 26 and connexin 32 was also observed . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| These cells also failed to maintain expression of the gap junction proteins connexin 26 and connexin 32 , leading to the loss of gap junctions . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Isoelectric points and post translational modifications of connexin 26 and connexin 32 . ^^^ The isoelectric points of the gap junction proteins connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) were determined by isoelectric focusing in free fluids . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Gap junctions between murine hepatocytes are composed of two subunit proteins , connexin 26 ( Cx 26 ) and connexin 32 ( Cx 32 ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Valve ICs did not express connexin 32 and 40 ; however , connexin 26 and 43 were equally expressed by a low percentage of ICs , demonstrating cell surface and cytoplasmic expression , and connexin 45 was weakly expressed . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Mutations in these genes cause autosomal recessive ( GJB 2 and GJB 3 ) , autosomal dominant ( GJB 2 , GJB 3 , and GJB 6 ) or 10 linked ( GJB 1 ) hearing impairment , both syndromic ( GJB 2 , keratoderma ; GJB 3 erythrokeratodermia variabilis ; and GJB 1 , peripheral neuropathy ) , and non syndromic ( GJB 2 , GJB 3 , and GJB 6 ) . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Mutations in GJB 1 , GJB 2 , GJB 3 and GJB 6 are involved in hearing impairment . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| Several connexin genes ( GJB 1 , GJB 2 , GJB 3 , GJB 6 and GJA 1 ) have been found mutated in patients with non syndromic and / or syndromic deafness indicating an important role of these proteins in the auditory system . ^^^ |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29033 and P08034 |
Pubmed |
SVM Score :0.0 |
| NA |
|