| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Neither anti LMP 2 nor anti LMP 7 sera displayed cross reactivity with the homologous proteasome subunits Delta and MB 1 . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Lmp 2 , Lmp 7 and Lmp 10 . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| The discovery that two proteasome beta subunits , delta and MB 1 , highly homologous to LMP 2 and LMP 7 and expressed in reciprocal manner , is now consistent with a role for the proteasome in antigen processing . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Lactacystin appears to modify covalently the highly conserved amino terminal threonine of the mammalian proteasome subunit 10 ( also called MB 1 ) , a close homolog of the LMP 7 proteasome subunit encoded by the major histocompatibility complex . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| By Western blot analysis , there were not consistent differences in the low molecular mass protein subunits of proteasomes ( lmp ) 2 , lmp 7 and delta , although the mb 1 proteasome subunit was regularly present at higher levels in group 1 BL lines relative to group 3 lines or LCL . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We have cloned the genes encoding a new proteasome subunit , MB 1 , which is closely related to LMP 7 , and that encoding a second subunit , Delta , which is closely related to LMP 2 . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| LMP 7 deficiency results in an enhanced incorporation of subunit MB 1 and in a 2 3 . 8 fold increase in Vmax for the Suc LLVY MCA hydrolyzing activity . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Two of the 14 proteasome subunits , delta and MB 1 , can be replaced by the major histocompatibility complex ( MHC ) encoded and interferon gamma ( IFN gamma ) inducible subunits LMP 2 and LMP 7 , respectively . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| There was no evidence of an alternative leader exon , which is a characteristic of the major histocompatibility complex ( MHC ) encoded LMP 7 gene , the closest relative of MB 1 , with which it shares 67 % amino acid identity . ^^^ None of the MB 1 gene ' s three exons were coincident with any of the six exons in LMP 7 . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| We show that six proteasome associated proteins are induced by IFN gamma , corresponding to three proteasome beta type subunits and their precursors : the MHC linked subunits ( LMP 2 and LMP 7 ) and LMP 10 . ^^^ Independent assortment of LMP 2 , LMP 7 , and LMP 10 into different proteasome complexes may thus generate up to 36 unique proteasome subsets . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| IFN gamma induces the synthesis of the beta subunits LMP 2 , LMP 7 and MECL 1 , which in consequence replace their constitutive subunit homologs delta , MB 1 and MC14 / Z in the 20S complex . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| A very similar reciprocal tissue expression was subsequently found for the homologous subunit pairs LMP 2 and delta as well as LMP 7 and MB 1 . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| First , gamma IFN induced replacements of the proteasomal subunits 10 , Y and Z by LMP 7 , LMP 2 and LMP 10 , respectively , producing `` immunoproteasomes ' ' that perhaps function more appropriate for the immunological processing of endogenous antigens . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| The assembly of precursor proteasomes occurs in at least two stages , with precursor beta subunits PSMB 2 ( C 7 1 ) , PSMB 3 ( C 10 2 ) , PSMB 7 ( Z ) , PSMB 9 ( LMP 2 ) and PSMB 10 ( LMP 10 ) being incorporated before others [ PSMB 1 ( C 5 ) , PSMB 6 ( delta ) , and PSMB 8 ( LMP 7 ) ] . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Catalytic properties of 26 S and 20 S proteasomes and radiolabeling of MB 1 , LMP 7 , and C 7 subunits associated with trypsin like and chymotrypsin like activities . 20 and 26 S proteasomes were isolated from rat liver . ^^^ In each case , label was found to be incorporated into subunits C 7 , MB 1 , and LMP 7 but in different relative amounts depending on the inhibitor used , consistent with the observed effects on the different peptidase activities . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Three of the 10 mammalian proteasome beta subunits ( LMP 2 , LMP 7 , and LMP 10 ) are induced by IFN gamma . ^^^ Northern analysis of Lmp 2 , Lmp 7 , and Lmp 10 showed expression in heart , liver , thymus , lung , and spleen , but not in brain , kidney , skeletal muscle , or testis . . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Accumulated evidence indicates that , upon stimulation with interferon gamma ( IFN gamma ) , three beta type subunits , designated LMP 2 , LMP 7 , and PSMB 10 , are incorporated into the 20S proteasome by displacing the housekeeping beta type subunits designated PSMB 6 , PSMB 5 , and PSMB 7 , respectively . ^^^ Its exon intron organization differs radically from those of the other IFN gamma regulated , beta type subunit genes including Lmp 7 with which Psmb 5 is believed to share an immediate common ancestor . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Coexpression of Lmp 2 and Lmp 7 with the constitutively expressed subunits delta and MB 1 was demonstrated in the BL lines by immunoprecipitation and two dimensional gel fractionation of the 20S proteasomes . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Interferons alpha and gamma had no effect on the composition of the 26 S proteasome , except for the replacement of subunits delta , MB 1 and Z with Lmp 2 , Lmp 7 and MECL 1 respectively . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| In some cases , virtually complete deficiencies were observed in the expression of the four genes encoded within the MHC ( TAP 1 , TAP 2 , LMP 2 , and LMP 7 ) , as well as LMP 10 , which is encoded outside the MHC . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Although in the tested samples expression of additional subunits of the proteasome , like LMP 2 , LMP 7 , and LMP 10 , were unaltered , the downregulation of TRAP 2 / p97 in tumor tissue might affect the processing and presentation of tumor associated antigens . . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Ritonavir is a reversible effector of proteasome activity that protected the subunits MB 1 ( 10 ) and / or LMP 7 from covalent active site modification with the vinyl sulfone inhibitor ( 125 ) 1 NLVS , suggesting that they are the prime targets for competitive inhibition by Ritonavir . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| The proteasome subunit varies minimally between cells with the exception of LMP 2 , LMP 7 , and LMP 10 subunits in rodent and human cells . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Thymus proteasome is characterized by the presence of LMP 2 , LMP 7 , and LMP 10 ( MECL 1 ) subunits , which replace the 10 , Y , and Z subunits . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Single or combined deficiencies in the expression and / or function of TAP , LMP 2 , LMP 10 and tapasin were demonstrated in 11 of 12 cell lines studied , whereas the expression of calnexin , calreticulin , beta 2 microglobulin , LMP 7 and PA28alpha was unaltered or only weakly decreased . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Formation of antigenic peptides by the multicatalytic proteinase complex ( MPC , proteasome ) is facilitated by incorporation of three subunits ( LMP 2 , LMP 7 and LMP 10 ) that are inducible by IFN gamma and TNF alpha . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| We postulate here that the MHC class 1 deficient phenotype of B 16 melanoma cells is attributable to down regulation or the loss of the expression and function of multiple components of the MHC class 1 antigen processing pathway , including the peptide transporter associated with antigen processing , the proteasome subunits LMP 2 , LMP 7 , and LMP 10 , PA28alpha and beta , and the chaperone tapasin . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Immunoblot analysis using antibodies to the beta subunits LMP 2 , LMP 7 and their constitutive counterparts delta and MB 1 revealed that two of the three major subtypes ( subtypes 1 and 2 ) are constitutive proteasomes , whereas two of the three minor subtypes belong to the subpopulation of immuno proteasomes . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| None of the activities of the bovine spleen MPC , an enzyme form in which the 10 , Y , and Z subunits are virtually completely replaced by the LMP 2 , LMP 7 , and LMP 10 subunits , was affected by either Hsp 90 or the other proteins tested . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Expression and regulation of interferon gamma inducible proteasomal subunits LMP 7 and LMP 10 in the bovine corpus luteum . ^^^ Expression of two INFgamma inducible proteasomal subunits , LMP 7 and LMP 10 , in bovine luteal tissue was examined in this study . ^^^ Northern analysis revealed the presence of mRNA encoding LMP 7 and LMP 10 in luteal tissue . ^^^ Steady state amounts of LMP 7 mRNA did not change during the estrous cycle , but LMP 10 mRNA was low in early corpus luteum ( CL ) and elevated in midcycle and late CL . ^^^ Tumor necrosis factor alpha alone and in the presence of LH and / or prostaglandin F2alpha elevated steady state amounts of LMP 10 mRNA but did not affect LMP 7 mRNA in cultured luteal cells . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| These included the genes coding for the low molecular weight proteins LMP 2 , LMP 7 , LMP 10 , the transporter associated with antigen processing ( TAP 1 , TAP 2 ) , and calnexin , calreticulin , tapasin , PA 28 alpha , PA 28 beta , ERP 59 and ER 60 . ^^^ Analyses with RT PCR showed that TAP 1 , TAP 2 , LMP 2 , LMP 7 , LMP 10 , tapasin and calnexin mRNA specific for these genes was absent in metastases produced in immunocompetent mice . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Loss of proteasome function was not associated with decreased proteasome content but with partial replacement of the proteolytic subunit PSMB 5 with the inducible subunit LMP 7 . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Natural selection during functional divergence to LMP 7 and proteasome subunit 10 ( PSMB 5 ) following gene duplication . ^^^ The LMP 7 and PSMB 5 genes were created through an ancient gene duplication event of their ancestral locus . ^^^ These proteins contain an active site of proteolysis , and LMP 7 replaces PSMB 5 as a component of the 20S proteasome after stimulation of cells by interferon gamma . ^^^ Replacement of PSMB 5 by LMP 7 changes the profile of the products of 20S proteasome processing , predisposing digested peptides for transport to and display by the immune system . ^^^ The purpose of this study is to investigate evolutionary forces influencing functional divergence between LMP 7 and PSMB 5 following duplication . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| When cells are incubated with interferon gamma , delta ( Y ) , MB 1 ( 10 ) , and Z are replaced by LMP 2 , LMP 7 , and LMP 10 , respectively , leading to the expression of immunoproteasome which generates peptides with increased affinity for HLA class 1 antigens . ^^^ To overcome this limitation , we generated human delta ( Y ) , MB 1 ( 10 ) , Z , LMP 2 , LMP 7 , and LMP 10 specific mAb secreting hybridomas from BALB / c mice immunized with peptides and recombinant fusion proteins . ^^^ The mAbs SY 5 , SJJ 3 , NB 1 , SY 1 , HB 2 , and TO 7 were shown to be specific for delta ( Y ) , MB 1 ( 10 ) , Z , LMP 2 , LMP 7 , and LMP 10 , respectively , as they react specifically with the corresponding molecules when tested with a human B lymphoid LG 2 cell lysate in Western blotting and with the peptide derived from each molecule in enzyme linked immunosorbent assay . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| In this article , we demonstrate that expression of several MHC class 1 APM components , including MB 1 ( beta 5 ) , LMP 2 , LMP 7 , LMP 10 , and ERp 57 , is significantly down regulated in human DC generated in the presence of primary oral squamous cell carcinoma cell lines or coincubated with purified gangliosides . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Expression of proteasomal subunits genes ( LMP 2 , LMP 7 and PSMB 5 ) involved in the MHC class 1 presentation pathway were modulated indicating the formation of immunoproteasomes improving antigen presentation . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Immunoproteasomes comprise a specialized subset of proteasomes that is defined by the presence of three catalytic immunosubunits : LMP 2 , MECL 1 ( LMP 10 ) , and LMP 7 . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Phylogenetic analyses of available sequences indicates that invertebrate LMP 10 like sequences are outgroups to vertebrate LMP 10 and LMP 7 , and is in agreement with previous observations that the duplication event giving rise to the two rapidly diverging lineages of proteasomal subunits occurred before jawed fished divergence . . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| We show in this report that JEV infection of mouse brain astrocytes results in induction of the nonclassical MHC Class Ib genes , H 2T23 , H 2Q4 and H 2T10 in addition to MHC 1 , Type 1 ( alpha / beta ) IFNs , TAP 1 , TAP 2 , Tapasin , LMP 2 , LMP 7 and LMP 10 but not IFNgamma , CD 80 , CD 86 and MHC 2 genes . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| Reanalyses of vertebrate PSMB 5 and PSMB 8 genes were conducted with a newly obtained amphioxus sequence . ^^^ The phylogenetic analysis indicated that the amphioxus sequence is an outgroup of both vertebrate PSMB 5 and PSMB 8 genes . ^^^ The agnathan sequences were previously thought to be closer to PSMB 5 than PSMB 8 sequences . ^^^ However , in this study the phylogenetic trees supported the clustering of agnathan sequences with PSMB 8 rather than with PSMB 5 sequences . ^^^ In the alignment there are many sites in which the agnathan sequences are more similar to PSMB 5 than PSMB 8 sequences . ^^^ |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28062 and P28074 |
Pubmed |
SVM Score :0.0 |
| NA |
|