| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.82753352 |
| We show that CREB binding protein ( CBP ) , through two cysteine histidine rich domains ( C / H1 and C / H3 ) , specifically and constitutively interacts with Pit 1 in pituitary cells . 0.82753352^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.86832251 |
| We determined that CBP interacts with Pit 1 and is a cofactor for Pit 1 dependent activation of the human GH promoter . 0.86832251^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| Furthermore , CREB binding protein also significantly increased activation by GHF 1 , and both proteins associated in vitro . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| Furthermore , CBP also increases the activation of the PRL promoter by GHF 1 and the ligand independent activation by both wild type and mutant VDR . . ^^^ Synergistic activation of the prolactin promoter by vitamin D receptor and GHF 1 : role of the coactivators , CREB binding protein and steroid hormone receptor coactivator 1 ( SRC 1 ) . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| Mutations in transcription factors have recently been identified in a number of genetic diseases ( for example , Denys Drash syndrome , WT 1 [ 19 ] ; pituitary dwarfism , PIT 1 [ 16 ] ; Rubinstein Taybi syndrome , CBP [ 20 ] . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| Here we show that whereas homeodomain containing repressors such as Rpx 2 seem to recruit only a co repressor complex , the activity of Pit 1 is determined by a regulated balance between a co repressor complex that contains N CoR / SMRT , mSin3A / B and histone deacetylases , and a co activator complex that includes the CREB binding protein ( CBP ) and p / CAF . ^^^ Activation of Pit 1 by cyclic AMP or growth factors depends on distinct amino and carboxy terminal domains of CBP , respectively . ^^^ Furthermore , the histone acetyltransferase functions of CBP or p / CAF are required for Pit 1 function that is stimulated by cyclic AMP or growth factors , respectively . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| While the CBP interaction domain of p / CIP inhibits acetylation of histones H 3 , H 4 , or high mobility group by CBP , it enhances acetylation of other substrates , such as Pit 1 . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| CBP and Pit 1 acted synergistically in TRH stimulation of the TSH beta promoter , and amino acids 1 450 of CBP were sufficient for the TRH effect . ^^^ Thus , the amino terminus of CBP plays a critical role in TRH signaling in the anterior pituitary via both Pit 1 dependent and independent pathways , yielding differential regulation of pituitary gene products . . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| In contrast , several CBP binding proteins , including phospho CREB , JUN / FOS , GATA 1 , Pit 1 , and EKLF , failed to stimulate HAT activity . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| We found that codon 24 proline in the transactivation domain as well as the POU domain of Pit 1 was crucial to recruit coactivator CREB binding protein ( CBP ) in the cultured cells . ^^^ Furthermore , CBP and Pit 1 , but not P24L , markedly enhanced the expression of the Pit 1 targeted gene to cAMP , and adenovirus E1a that binds to CBP and abrogates its function blocked the induction by cAMP of Pit 1 stimulated gene transcription in the pituitary derived GH 3 cells . ^^^ These results suggest that CBP and proline at codon 24 in the transactivation domain of Pit 1 are important for the cAMP induced activation of Pit 1 targeted genes . ^^^ However , P24L maintained basal transcriptional activity , suggesting that CBP is unlikely to be an essential coactivator for Pit 1 . . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| In order to explore these differences , we : ( 1 ) identified the transcriptional cofactors necessary for reconstitution of repression in non pituitary cells ; ( 2 ) tested the effect of the beta domain on heterodimerization with Pit 1 and physical interaction with the co activator CREB binding protein ( CBP ) ; and ( 3 ) determined the beta domain sidechain chemistry requirements for repression . ^^^ The beta domain allowed heterodimerization with Pit 1 but blocked physical interaction with CBP , and specific chemical properties of the beta domain beyond hydrophobicity were dispensable . ^^^ These data strongly suggest that Pit 1beta represses hormone gene expression by heterodimerizing with Pit 1 and interfering with the assembly of the Pit 1 CBP complex required for PRL promoter activity in pituitary cells . . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| Age , mechanism of injury , Physiologic Index ( PI ) on admission , site of injury , associated injuries , ISS , RTS , Penetrating Cardiac Trauma Index ( PCTI ) , Penetrating Thoracic Trauma Index ( PTTI ) , Penetrating Trauma Index ( PTI ) , TRISS and mortality rate were reviewed . ^^^ RTS , PI , PCTI , PTTI , PTI , and ISS reached statistical significance when comparing survivors and nonsurvivors . ^^^ Trauma indices such as PI , RTS , PCTI , PTTI , PTI , and ISS are good predictors of outcome . ^^^ |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P28069 and Q92793 |
Pubmed |
SVM Score :0.0 |
| NA |
|