Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.75630332
In non pituitary cell lines , coexpression of ERF disrupts the cooperative interactions between Pit 1 and ETS 1 and blocks the induction of Pit 1 dependent prolactin promoter activity by cAMP . 0.75630332^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.62346545
We mapped the minimal region of Ets 1 required for the interaction with the Pit 1 POU homeodomain . 0.62346545^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
GHF 1 / Pit 1 functions as a cell specific integrator of Ras signaling by targeting the Ras pathway to a composite Ets 1 / GHF 1 response element . ^^^ Using a series of site specific mutations and deletions of the proximal rPRL promoter we have mapped the major Ras / Raf response element ( RRE ) to a composite Ets 1 / GHF 1 binding site located between positions 217 and 190 . ^^^ Mutation of either the Ets 1 or GHF 1 binding sites inhibits Ras and Raf activation of the rPRL promoter , and insertion of this RRE into the rat growth hormone promoter confers Ras responsiveness . ^^^ We show that Ets 1 is expressed in GH 4 cells and , consistent with their functional synergistic interaction , both Ets 1 and GHF 1 are able to bind specifically to this bipartite RRE . ^^^ Thus , the pituitary specific POU homeodomain transcription factor , GHF 1 , serves as a cell specific signal integrator by functionally interacting with an Ets 1 like factor , at uniquely juxtaposed binding sites , thereby targeting an otherwise ubiquitous Ras signaling pathway to a select subset of cell specific GHF 1 dependent genes . . ^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
Interaction of Ets 1 and the POU homeodomain protein GHF 1 / Pit 1 reconstitutes pituitary specific gene expression . ^^^ Here we identify Ets 1 as a factor that functionally and physically interacts with GHF 1 to fully reconstitute proximal PRL promoter activity . ^^^ The Ets 1 GHF 1 synergy requires a composite Ets 1 GHF 1 cis element and is dependent on an Ets 1 specific protein domain . ^^^ Finally , Ets 1 , but not Ets 2 , binds directly to GHF 1 and GHF 2 . ^^^ These data show that a functional interaction of GHF 1 and Ets 1 , acting via a composite DNA element , is required to establish lactotroph specific PRL gene expression , thus providing a molecular mechanism by which GHF 1 can discriminate between the GH and PRL genes . ^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
However , overexpression of Ets 1 and GHF 1 , which potentiate the Ras response , inhibits FGF stimulation of the rPRL promoter , implying that Ras and FGF signaling pathways target distinct factors to elicit their effects . ^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
Pit 1beta / GHF 2 , a distinct splice isoform of Pit 1 , acts to inhibit Ras activated transcription from the rPRL promoter , which is mediated by a functional interaction between Pit 1 and Ets 1 at the most distal Pit 1 binding site ( FP 4 ) . ^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
Although pit 1 was 1 of the first factors identified as a cause of CPHD in mice , many other homeodomain and transcription factors have been characterized as being involved in different developmental stages of pituitary gland development , such as prophet of pit 1 ( prop 1 ) , P Lim , ETS 1 , and Brn 4 . ^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
The Pit 1 homeodomain and beta domain interact with Ets 1 and modulate synergistic activation of the rat prolactin promoter . ^^^ Synergistic interaction of Pit 1 with a member of the Ets family of transcription factors , Ets 1 , has been shown to be an important mechanism regulating basal and Ras induced lactotroph specific rat ( r ) PRL promoter activity . ^^^ By using a series of Pit 1 internal deletion constructs in a transient transfection protocol to reconstitute rPRL promoter activity in HeLa cells , we have determined that the functional and physical interaction of Pit 1 and Ets 1 is mediated via the POU homeodomain , which is common to both Pit 1 and Pit 1beta . ^^^ Although the Pit 1 homeodomain is both necessary and sufficient for direct binding to Ets 1 in a DNA independent manner , an additional interaction surface was mapped to the beta domain , specific to the Pit 1beta isoform . ^^^ Thus , the unique transcriptional properties of Pit 1 and Pit 1beta on the rPRL promoter may be due to the formation of functionally distinct complexes of these two Pit 1 isoforms with Ets 1 . . ^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
Ras signaling and transcriptional synergy at a flexible Ets 1 / Pit 1 composite DNA element is defined by the assembly of selective activation domains . ^^^ Pit 1 and Ets 1 binding to a composite element synergistically activates and targets Ras mitogen activated protein kinase signaling to the rat prolactin promoter . ^^^ These transcriptional responses appear to depend on three molecular features : organization of the Ets 1 / Pit 1 composite element , physical interaction of these two factors via the Pit 1 homeodomain ( amino acids 199 291 ) and the Ets 1 regulatory 3 domain ( amino acids 190 257 ) , and assembly of their transcriptional activation domains ( TADs ) . ^^^ Here we show that the organization of the Ets 1 / Pit 1 composite element tolerates significant flexibility with regard to Ras stimulation and synergy . ^^^ Additionally , we show that the physical interaction of Ets 1 and Pit 1 is not required for Ras responsiveness or synergy because block mutations of the Pit 1 interaction surface in Ets 1 , which reduced Ets 1 / Pit 1 binding in vitro , did not significantly affect Ets 1 stimulation of Ras responsiveness or synergy . ^^^
Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
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Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
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Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
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Interacting proteins: P28069 and P14921	Pubmed	SVM Score :0.0
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