Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Scaffolding adapter Grb 2 associated binder 2 ( Gab 2 ) is a key component of FcepsilonRI signaling in mast cells , required for the activation of PI3K . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Instead , a 100 kDa tyrosine phosphorylated protein ( pp 100 ) co immunoprecipitated with the regulatory subunit of PI3K . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Ligand stimulation of neuronal cells induced the assembly of a large protein complex containing c Ret , Grb 2 , and tyrosine phosphorylated forms of Shc , p 85 ( PI3K ) , the adaptor Gab 2 , and the protein tyrosine phosphatase SHP 2 . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Here , we show that in TCR signaling , the scaffolding adapter Gab 2 delivers an inhibitory signal via PI3K . ^^^ Inhibition is abrogated by mutating the Gab 2 p85 binding sites , by treatment with PI3K inhibitors or by cotransfection of phosphatase homolog of tensin . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
The docking proteins that the BCR uses to recruit PI3K include CD 19 , Cbl , Gab 1 , and perhaps Gab 2 . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Gab 2 becomes tyrosine phosphorylated in response to a variety of growth factors and forms multimolecular complexes with SH 2 domain containing signaling molecules such as the p 85 regulatory subunit of the phosphoinositide 3 kinase ( p 85 PI3K ) , the tyrosine phosphatase SHP 2 and the adapter protein CrkL . ^^^ Interaction with p 85 PI3K is mediated by tyrosine residues Y 452 , Y 476 and Y 584 of Gab 2 , while interaction with SHP 2 depends exclusively on tyrosine Y 614 . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Similar to cells expressing full length receptor , PI3K was activated by its incorporation into a complex with IRS 2 or Gab 2 . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
We demonstrated that 5 SEA activates the PI3K Akt signaling pathway primarily in Y 557 and secondarily in Y 564 dependent manner . 5 SEA was also shown to induce the tyrosine phosphorylation of the Gab 2 protein , leading to PI3K association and thus providing an alternative route for PI3K activation . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Compared to BCR / ABL expressing Ba / F3 cells , BCR / ABL Y177F cells exhibit markedly reduced Gab 2 tyrosine phosphorylation and association of phosphatidylinositol 3 kinase ( PI3K ) and Shp 2 with Gab 2 and BCR / ABL , and decreased PI3K / Akt and Ras / Erk activation , cell proliferation , and spontaneous migration . ^^^ BCR / ABL evoked PI3K / Akt and Ras / Erk activation also are impaired in Gab 2 ( / ) primary myeloid and lymphoid cells . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
In these cells , three major p 85 containing complexes were formed after EGF treatment : ErbB 3 p85 , Shc p 85 , and a multimeric Gab 2 Grb2 SHP 2 p85 , which accounted for more than 80 % of total EGF induced PI3K activity ( Kong , M . , C . ^^^ Overexpression of the PH domain of Gab 2 did not affect EGF induced Gab 2 phosphorylation , PI3K activation , and DNA synthesis . ^^^ In summary , after EGF stimulation , ErbB 3 , Shc , and Gab 2 are differentially compartmentalized in rat liver , where they associate with and activate PI3K . ^^^ Our data demonstrate that Gab 2 mediates EGF induced PI3K activation and DNA synthesis in a PH domain independent manner . . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
We show that association of c Met with PI3K and GAB 2 is diminished by inhibiting c Met . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Upon FcgammaR activation , Gab 2 becomes tyrosyl phosphorylated and associated with p 85 , the regulatory subunit of phosphoinositide 3 kinase ( PI3K ) , and the protein tyrosine phosphatidylinositol Shp 2 . ^^^ Using confocal fluorescence microscopy , we find that Gab 2 is recruited to the nascent phagosome , where de novo PI3K lipid production occurs . ^^^ Gab 2 recruitment requires the pleckstrin homology domain of Gab 2 and is sensitive to treatment with the PI3K inhibitor wortmannin . ^^^ Because PI3K activity is required for FcgammaR mediated phagocytosis , our results indicate that Gab 2 acts as a key component of FcgammaR mediated phagocytosis , most likely by amplifying PI3K signaling in the nascent phagosome . . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Using mutant forms of Gab 2 , we show that activation of the PI3K Akt pathway via Gab 2 is required for 5 SEA induced transformation . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Immunoprecipitations showed that ErbB 3 and Grb 2 associated binder ( Gab ) 1 were phosphorylated and associated with PI3K activity after heregulin treatment and that Gab 1 and Gab 2 , but not ErbB 3 , were phosphorylated and associated with PI3K activity after EGF treatment . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
Overexpression of Gab 2 3YF , a mutant of Gab 2 incapable of binding PI3K , inhibited the proliferation and survival of caSTAT 5 expressing cells as well as ERK1 / 2 and Akt / protein kinase B phosphorylation . ^^^ Taken together , our results indicate that Gab 2 is required for caSTAT 5 induced cell proliferation by regulating both the PI3K / Akt and the Ras / MAPK pathways . . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
The phosphorylation of Gab 2 , Akt and Vav was also inhibited by WHI P 131 and WHI P 154 , indicating that these inhibitors suppress the activation of phosphatidylinositol 3 kinase ( PI3K ) . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
The interaction between p85alpha SH 3 and BCR / ABL may be intermediated by proteins such as c Cbl , Shc , Grb 2 , and / or Gab 2 . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
The adaptor protein Gab 2 coordinates the assembly of the IL 3 signalsome comprising Gab 2 , Grb 2 , Shc , SHP 2 and PI3K . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
In this study , we show that ligation of CD 28 by its natural ligand B 7 1 / CD80 , induces tyrosine phosphorylation of Gab 2 and its coassociation with Src homology phosphatase ( SHP ) 2 and class IA PI3K in Jurkat cells . ^^^ To characterize this inhibitory function further , we used Gab 2 mutants unable to bind either PI3K or SHP 2 and a PH domain deletion mutant . ^^^ Although PI3K has previously been implicated as necessary for Gab 2 mediated inhibition of TCR signaling , Gab 2 mutants defective in their ability to bind PI3K or SHP 2 retained their inhibitory function , whereas deletion of the PH domain ablated the inhibitory effect of Gab 2 . ^^^ Together , these data demonstrate that CD 28 stimulation of T cells is sufficient to induce an inhibitory multimeric signaling complex involving Gab 2 , SHP 2 , and PI3K . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
The E76K mutation in the N terminal Src homology 2 domain increased interactions of mutant SHP 2 with Grb 2 , Gab 2 , and p 85 , leading to hyperactivation of IL 3 induced Erk and phosphatidylinositol 3 kinase ( PI3K ) pathways . ^^^ Furthermore , catalytically inactive SHP 2 E76K with an additional C459S mutation retained the capability to increase the interaction with Gab 2 and to enhance the activation of the PI3K pathway . ^^^
Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UQC2 and P27986	Pubmed	SVM Score :0.0
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