| Treatment of cells with LY 294002 , an inhibitor of phosphatidylinositol 3 kinase ( PI3K ) , also abrogated the PDGF induced lamellipodia formation and cell migration , suggesting that PI3K may be required for WAVE 3 activity . ^^^ WAVE 3 and the PI3K regulatory subunit , p 85 , were found to interact in a yeast two hybrid screen , which was confirmed through co immunoprecipitation . ^^^ These results imply that the WAVE 3 mediated migration in MDA MB 231 cells via lamellipodia formation is activated downstream of PI3K and induced by PDGF . ^^^ |