Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
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Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.62829184
Association between PI3K , PYK 2 , and the beta 1 integrin subunit were also evaluated in co immunoprecipitation experiments . 0.62829184^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Protein kinase B ( PKB ) is a downstream target of PI3K activation by growth factors . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Serine / threonine kinase Akt ( protein kinase B ; PKB ) is downstream of PI3K and activated by growth factors . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PI3K dependent activation of PKB is only lost in Syk deficient lines . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Regulation of cell size in growth , development and human disease : PI3K , PKB and S6K . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Activities of PI3K downstream target PKB under hypoxia and normoxia were comparable . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Akt , also known as protein kinase B ( PKB ) , is a serine / threonine kinase downstream target of PI3K . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Here , we show that Survivin is controlled in peripheral T cells by OX 40 cosignaling via sustained PI3k and PKB activation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The conical signaling cascade initiated by growth factors is mediated by PI3K , PKB , TSC1 / 2 and Rheb . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The insulin induced PKCdelta PKB association was PI3K dependent . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Furthermore we identified PI3K as an activator of PKC zeta , and Pyk 2 and ERK1 / 2 as downstream targets of PKC zeta . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In apoptotic cells , the expressions of the PYK 2 , p p 38 , PI3K and Grb 2 adhesion proteins were increased . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In this study , we demonstrate that RAFTK is co immunoprecipitated with phosphoinositide 3 kinase ( PI3K ) upon platelet activation , and that thrombin , ADP and collagen induced the phosphorylation of both PI3K and RAFTK . ^^^ A low dose of thrombin ( 0 . 015 U / ml ) induced RAFTK phosphorylation and platelet aggregation in a PI3K activity dependent manner , whereas a high dose of thrombin ( 0 . 1 U / ml ) induced these events in a PI3K activity independent manner . ^^^ ADP and collagen also induced RAFTK phosphorylation and platelet aggregation in a PI3K activity dependent manner , similar to that of the low dose thrombin . ^^^ The association of SHP 2 with RAFTK was PI3K dependent and was increased upon RAFTK phosphorylation . ^^^ Taken together , our results strongly suggest that the involvement of RAFTK in platelet activation is mediated via the PI3K pathway . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Protein kinase B ( PKB ) is activated by IL 2 via PI3K . ^^^ These results establish a crucial PI3K / PKB mediated link between the IL 2 teceptor and the cell cycle machinery . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Recently , the protein kinase PKB / akt ( referred to here as PKB ) has been identified as a downstream target of PI3K necessary for survival . ^^^ We have shown that granulocyte / macrophage colony stimulating factor ( GM CSF ) maintains survival in the absence of PI3K activity , and we now show that when PKB activation is also completely blocked , GM CSF is still able to stimulate phosphorylation of Bad . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
When the expression levels of the insulin activated kinases were probed with specific antibodies in ventricular extracts from 1 , 10 , 20 , 50 , and 365 day old rats , phosphatidylinositol 3 kinase ( PI3K ) , PKB , S6K , and CK 2 were downregulated ( 40 60 % ) with age . ^^^ Compared to other adult rat tissues such as brain and liver , the levels of PI3K , PKB , S6K , and GSK3beta were relatively low in the heart . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Recent studies indicate that phosphatidylinositide 3OH kinase ( PI3K ) induced S 6 kinase ( S6K1 ) activation is mediated by protein kinase B ( PKB ) . ^^^ Support for this hypothesis has largely relied on results obtained with highly active , constitutively membrane localized alleles of wild type PKB , whose activity is independent of PI3K . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
To address this issue , we have used three different factor dependent hemopoietic cell lines , MC / 9 , BaF / 3 , and factor dependent ( FD ) 6 , which respond to a range of cytokines , to investigate the relationship between PI3K , PKB , and Bad activity with survival . ^^^ In all cases , cytokine induced PKB activation was sensitive to inhibition by the PI3K inhibitor , LY 294002 . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
ANG 2 stimulated Akt / PKB activation was inhibited by the tyrosine kinase inhibitors genistein and herbimycin A and by the phosphatidylinositol 3 kinase ( PI3K ) inhibitors wortmannin and LY 294002 . ^^^ These results indicate that ANG 2 stimulates Akt / PKB activity via AT 1 receptors in VSMC and that the activities of tyrosine kinase and PI3K are required for this activation . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Membrane translocation of PKB and subsequent PKB activation are dependent on BCR activation of phosphatidylinositol 3 kinase ( PI3K ) . ^^^ Moreover , PI3K signals are both necessary and sufficient for sustained activation of PKB in B lymphocytes . ^^^ However , under conditions of continuous PI3K activation or BCR triggering there is only transient recruitment of PKB to the plasma membrane , indicating that there must be a molecular mechanism to dissociate PKB from sites of PI3K activity in B cells . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Phosphatidylinositol 3 kinase ( PI3K ) , which lies upstream of PKB , was increased approximately 3 . 5 fold within 2 5 min by insulin in control rats . ^^^ In summary , 1 ) the PI3K > PKB > S6K pathway was upregulated under basal conditions , and 2 ) insulin stimulation of PI3K and S6K activities was enhanced , but both PKB and GSK 3 were refractory to the effects of insulin in FF rats . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
However , the involvement of protein kinase B ( PKB ) / Akt , a downstream target of PI3K in regulation of GLUT 4 translocation , has been controversial . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Because phosphatidylinositol 3 kinase ( PI3K ) and protein kinase B ( PKB ) , in concert with mTOR , inactivate 4E BP 1 , we explored their role in GLUT 1 protein expression . ^^^ Cotransfection of cytomegalovirus promoter driven , hemagglutinin epitope tagged GLUT 1 with dominant inhibitory mutants of PI3K or PKB inhibited the insulin elicited increase in hemagglutinin tagged GLUT 1 protein . ^^^ Increased GLUT 1 mRNA translation appears to occur via the PI3K / PKB / mTOR / 4E BP 1 cascade . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Active forms of PKB as well as of its upstream activator phosphatidylinositol 3 kinase ( PI3K ) have been found to be responsible for the transforming activities of certain viruses , and the negative regulator of this pathway , PTEN , is a tumor suppressor . ^^^ The identification of particular downstream targets of PKB has provided us with new insights into the possible mechanism of PI3K / PKB mediated tumorigenicity . ^^^ Recently a subfamily of Forkhead transcription factors was identified as additional targets for PI3K / PKB signaling . ^^^ This review discusses the studies that have led to this conclusion and the possible implications of this finding for our understanding of how PI3K / PKB activity could lead to oncogenesis . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Role of the PI3K / PKB signaling pathway in cAMP mediated translocation of rat liver Ntcp . cAMP stimulates Na ( + ) taurocholate ( TC ) cotransport by translocating the Na ( + ) TC cotransporting peptide ( Ntcp ) to the plasma membrane . ^^^ Together , these results suggest that the stimulation of TC uptake and Ntcp translocation by cAMP may be mediated via the PI3K / PKB signaling pathway and requires intact actin filaments . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In order to study the role of phosphatidylinositol 3 kinase ( PI3K ) , PKB , FRAP , S 6 kinase , and MAP kinase in insulin stimulated glycogen synthesis , we used a specific inhibitor of PI3K , LY 294002 , the immunosuppressant inhibitor of FRAP , rapamycin , and the inhibitor of MAPK kinase ( MEK ) / MAPK , PD 98059 , in rat HTC hepatoma cells overexpressing human insulin receptors . ^^^ The PI3K inhibitor LY 294002 completely blocks insulin stimulated glycogen synthesis by inhibiting glycogen synthase , PKB ( Akt 1 ) , and FRAP ( RAFT ) autophosphorylation , as well as p 70 S6 kinase activation , whereas insulin receptor substrates tyrosine phosphorylation and MEK activity were not affected . ^^^ However , the inhibition of FRAP and S 6 kinase activation is not sufficient to block insulin stimulated glycogen synthesis , suggesting an important role of a branching pathway upstream of S 6 kinase and downstream of PI3K , which is probably mediated by PKB in the signaling of the insulin receptor in hepatoma HTC cells . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The PI3K PDK 1 connection : more than just a road to PKB . ^^^ One of the best characterized targets of PI3K lipid products is the protein kinase Akt or protein kinase B ( PKB ) . ^^^ Upon cellular stimulation , PKB is activated through recruitment to cellular membranes by PI3K lipid products and phosphorylation by 3 ' phosphoinositide dependent kinase 1 ( PDK 1 ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The anti apoptotic effect of PI3K is realized by activation of proteins from another signaling pathway protein kinase B ( PKB ) and / or PKB dependent enzymes ( GSK 3 , ILK ) . ^^^ The transforming effect of PI3K is supposed to occur on the basis of complex alterations in cellular signaling pathways : appearance of constitutively generated PI3K dependent mitogen signal and activation of some protooncogenes ( src , ras , rac , etc . ) , PI3K / PKB pathway stimulation resulting in delay of apoptosis and increase of cell survival , and actin cytoskeleton reorganization . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Activation of PI3K leads to enhanced activity of PKB and increased levels of the anti apoptotic protein Bcl 10 ( L ) . ^^^ Thus , PI3K and PKB are required for the anti apoptotic effects of Abl PTK . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In primary hepatocytes isolated from null mice , expression of IRS 1 enhanced both PI3K and PKB activities , but expression of IRS 1Deltap85 enhanced only PKB . ^^^ These data suggest that PKB in liver plays a pivotal role in systemic glucose homeostasis and that PKB activation might be sufficient for reducing insulin resistance even without full activation of PI3K . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The inhibitory effects of the overexpressed JM domain were accompanied by inhibition of insulin stimulated IRS 1 phosphorylation , decreased IRS 1 associated PI3K activity , and decreased phosphorylation of the downstream effectors of PI3K , PKB and p 70 S6K . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Stimulation of phosphatidylinositol 3 ' kinase ( PI3K ) and protein kinase B ( PKB ) is implicated in the regulation of protein synthesis in various cells . ^^^ One mechanism involves PI3K / PKB dependent phosphorylation of 4E BP 1 , which dissociates from eIF4E , allowing initiation of translation from the 7 methylGTP cap of mRNAs . ^^^ Insulin or H ( 2 ) O ( 2 ) increased the phosphorylation ( activation ) of PKB through PI3K , but , whereas insulin induced a sustained response , the response to H ( 2 ) O ( 2 ) was transient . 4E BP 1 was phosphorylated in unstimulated cells , and 4E BP 1 phosphorylation was increased by insulin . ^^^ These results indicate that PI3K and PKB are important regulators of protein synthesis in cardiac myocytes , but other factors , including phosphatase activity , modulate the overall response . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Ser ( 136 ) is thought to be phosphorylated by protein kinase B ( PKB , also called Akt ) , which is activated when cells are exposed to agonists that stimulate phosphatidylinositol 3 kinase ( PI3K ) . ^^^ However insulin like growth factor 1 , a potent activator of PI3K and PKB does not increase the phosphorylation of Ser ( 136 ) in BAD transfected HEK 293 cells , and nor is the basal level of Ser ( 136 ) phosphorylation suppressed by inhibitors of PI3K . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
There is growing evidence that epidermal growth factor ( EGF ) activates Akt / protein kinase B ( PKB ) in a phosphoinositide 3 OH kinase ( PI3K ) dependent manner , but it is not yet clear which Akt isoforms are involved in this signal transduction pathway . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
However , through PI3K , SCF caused activation of protein kinase B ( PKB ) , an anti apoptosis signal , whereas EPO led to activation of ERKs . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PD 98059 , the specific inhibitor of MEK 1 , significantly blocks EGF induced DNA synthesis , whereas wortmannin , the PI3K inhibitor , exerts a modest inhibitory effect , which suggests that the activation of MEK 1 MAPK pathway plays a major role in EGF induced DNA synthesis and the activation of PI3K PKB pathway plays a minor role . ^^^ Although we found that EGF stimulated p70s6K phosphorylates through a MAPK dependent and a MAPK independent ( wortmannin sensitive ) pathway , TGF beta 1 failed to block EGF triggered phosphorylation of p70s6K at thr ( 389 ) and thr ( 421 ) / ser ( 424 ) sites , implying that PKB inhibition by TGF beta 1 may result from inhibition of PDK 1 activity instead of inhibition of PI3K activity . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PGE ( 2 ) could stimulate the activation of PKB , which was sensitive to phosphatidylinositol 3 OH kinase ( PI3K ) inhibitor , wortmannin . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The tumor suppressor protein PTEN is mutated in glioblastoma multiform brain tumors , resulting in deregulated signaling through the phosphoinositide 3 kinase ( PI3K ) protein kinase B ( PKB ) pathway , which is critical for maintaining proliferation and survival . ^^^ We have examined the relative roles of the two major phospholipid products of PI3K activity , phosphatidylinositol 3 , 4 biphosphate [ PtdIns ( 3 , 4 ) P 2 ] and phosphatidylinositol 3 , 4 , 5 triphosphate [ PtdIns ( 3 , 4 , 5 ) P 3 ] , in the regulation of PKB activity in glioblastoma cells containing high levels of both of these lipids due to defective PTEN expression . ^^^ Reexpression of PTEN or treatment with the PI3K inhibitor LY 294002 abolished the levels of both PtdIns ( 3 , 4 ) P 2 and PtdIns ( 3 , 4 , 5 ) P 3 , reduced phosphorylation of PKB on Thr 308 and Ser 473 , and inhibited PKB activity . ^^^ Our results suggest that SHIP 2 plays a negative role in regulating the PI3K PKB pathway . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Furthermore , Western blotting showed that the reduction of phosphorylated PKB / Akt levels correlated with the enhancement of gemcitabine induced apoptosis , suggesting that the PI3K PKB / Akt pathway plays a significant role in mediating drug resistance in human pancreatic cancer cells . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Here , we report the regulation of host cell phosphatydilinositol 3 kinase ( PI3K ) and protein kinase B ( PKB / Akt ) activities by T . cruzi during parasite host cell interaction . ^^^ A strong activation of PI3K and PKB / Akt activities in Vero cells was detected when these cells were incubated with trypomastigotes or their isolated membranes . ^^^ Consistently , we were unable to detect activation of PI3K or PKB / Akt activities in host cells during epimastigote ( noninfective ) membrane host cell interaction . ^^^ Taken together , these results indicate that PI3K and PKB / Akt activation in parasites , as in host cells induced by T . cruzi , is an early invasion signal required for successful trypomastigote internalization . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Examination of the signaling components downstream of PI3K , 3 phosphoinositide dependent kinase 1 , protein kinase B ( PKB ) , glycogen synthase kinase 3 , and p 70 kDa S 6 kinase ( p 70 ( S6K ) ) , suggested that a major part of glucose dependent beta cell proliferation requires activation of mammalian target of rapamycin / p70 ( S6K ) , independent of phosphoinositide dependent kinase 1 / PKB activation . ^^^ However , for IGF 1 induced activation , these pathways must be carefully balanced , because chronic activation of one ( PI3K / PKB ) can lead to dampening of the other ( ERK1 / 2 ) , reducing the mitogenic response . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Although both the phospho ( CK 2 PVn ) and non phospho ( Vn ) analogs of vitronectin ( simulated by mutants Vn ( T50E , T57E ) , and Vn ( T50A , T57A ) , respectively ) trigger the alpha ( 5 ) beta 3 as well as the alpha ( 5 ) beta 5 integrins , and equally activate the ERK pathway , these two forms are different in their activation of the focal adhesion kinase / phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) pathway . ^^^ Specifically , we show ( 1 ) that , upon exposure of cells to Vn / CK2 PVn , their PKB activation depends on the availability of the alpha ( 5 ) beta 3 integrin on their surface ; ( 2 ) that upon adhesion of the beta 3 transfected cells onto the CK 2 PVn , the extent of PKB activation coincides with the enhanced adhesion of these cells , and ( 3 ) that both the PKB activation and the elevation in the adhesion of these cells is PI3K dependent . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The PI3K inhibitors wortmannin and LY 294002 stimulated stress activated protein kinase / c Jun NH ( 2 ) terminal kinase ( SAPK / JNK ) and p 38 mitogen activated protein kinase ( MAPK ) phosphorylation in a rapid and concentration dependent manner that paralleled the inhibition of protein kinase B ( PKB ) phosphorylation . ^^^ Although PI3K inhibitors reversed the suppressive effects of insulin on CYP2E1 expression , these effects only occurred at concentrations well in excess of those required to achieve complete inhibition of PKB phosphorylation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Phosphoinositide dependent kinase 1 ( PDK 1 ) is a central mediator of the cell signaling between phosphoinositide 3 kinase ( PI3K ) and various intracellular serine / threonine kinases including Akt / protein kinase B ( PKB ) , p 70 S6 kinases , and protein kinase C . ^^^ Conversely , overexpression of dPDK 1 increased cell and organ size in a Drosophila PI3K dependent manner . dPDK 1 not only could activate Drosophila Akt / PKB ( Dakt 1 ) , but also substitute the in vivo functions of its mammalian ortholog to activate Akt / PKB . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
We suggest that PKB and PhdA lie downstream from PI3K and control different downstream effector pathways that are essential for proper chemotaxis . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
These changes include : ( 1 ) activation of phosphatidylinositol 3 kinase ( PI3K ) and production of PIP 3 ; ( 2 ) PIP 3 dependent activation of atypical protein kinase Cs ( PKCs ) ; ( 3 ) PIP 3 dependent activation of PKB ; ( 4 ) PI3K dependent activation of phospholipase D and hydrolysis of phosphatidylcholine with subsequent increases in phosphatidic acid ( PA ) and diacylglycerol ( DAG ) ; ( 5 ) PI3K independent activation of glycerol 3 phosphate acylytansferase and increases in de novo synthesis of PA and DAG ; and ( 6 ) activation of DAG sensitive PKCs . ^^^ Recent findings suggest that atypical PKCs and PKB serve as important positive regulators of insulin stimulated glucose metabolism , whereas mechanisms that result in the activation of DAG sensitive PKCs serve mainly as negative regulators of insulin signaling through PI3K . ^^^ Atypical PKCs and PKB are rapidly activated by insulin in adipocytes , liver , skeletal muscles , and other cell types by a mechanism requiring PI3K and its downstream effector , 3 phosphoinositide dependent protein kinase 1 ( PDK 1 ) , which , in conjunction with PIP 3 , phosphorylates critical threonine residues in the activation loops of atypical PKCs and PKB . ^^^ Together , atypical PKCs and PKB serve as a potent , integrated PI3K / PDK 1 directed signaling system that is used by insulin to regulate glucose metabolism . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In our study , even a short term exposure of transformed fibroblasts of hamster ( line 2PK ) resulted in an activation of main PI3K effectors ( MAP kinase and protein kinase B ( PKB ) ) which appeared against the background of hormone induced inhibition of cellular growth . ^^^ A long term ( 3 months ) cell culturing with dexamethasone was followed by formation of subpopulations of cells which were refractory to the growth inhibition by hormone and were characterized by high levels of activity of PI3K , MAP kinases and PKB . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
We demonstrate that PI3K and PKB mediate PAKa ' s subcellular localization , PAKa ' s activation in response to chemoattractant stimulation , and chemoattractant mediated myosin 2 assembly . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Whereas insulin gene transcription is promoted by signaling through insulin receptor A type ( Ex 11 ) , PI3K class Ia , and p70s6k , insulin stimulates the betaGK gene by signaling via insulin receptor B type ( Ex11+ ) , PI3K class 2 like activity , and PKB ( c Akt ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In this paper , we provide evidence that IL 2 mediated induction of the c myb gene occurs via the phosphoinositide 3 kinase ( PI3K ) signaling pathway , that protein kinase B ( PKB ) is the principal transducer of this signal , and that activation of the c myb promoter can be abolished by deletion of conserved E2F and NF kappaB binding sites . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Moreover , analysis of the levels and subcellular distribution of insulin receptor substrates 1 and 2 , the p85alpha subunit of phosphatidylinositol 3 ' kinase , and protein kinase B ( PKB ) / cAKT in skeletal muscle did not identify any differences between the two strains ; the insulin dependent activation of PKB / cAKT was not different between the two strains . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In the present study , we examined whether insulin / IGF 1 stimulation caused activation of the phosphoinositide 3 kinase ( PI3K ) / protein kinase B ( PKB ) pathway in ATDC 5 cells . ^^^ These results support the hypothesis that the PI3K / PKB signaling pathway is involved in the chondrogenic differentiation of ATDC 5 cells in response to insulin / IGF 1 stimulation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Accordingly , we found that Alb AGE activated mitogen activate protein kinase , ERK1 / 2 , JNK1 / 2 , but not p 38 , and that Alb AGE did not activate PI3K and PKB . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Taken together , our results demonstrate a critical role for Src kinases in regulating neutrophil cytotoxic effector functioning through PI3K PKB . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Survival signals elicited by cytokines include the activation of phosphatidylinositol 3 kinase ( PI3K ) , which in turn promotes the activation of protein kinase B ( PKB ) . ^^^ Furthermore , hematopoietic stem cells isolated from Bim / mice exhibited reduced levels of apoptosis upon inhibition of PI3K / PKB signaling . ^^^ Thus PI3K / PKB mediated inhibition of this transcription factor likely provides an important mechanism by which survival factors act to prevent programmed cell death . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Here we have examined the regulation of protein kinase B ( PKB ) , a downstream effector of PI3K , by the platelet collagen receptor glycoprotein ( GP ) 6 and thrombin receptors . ^^^ Stimulation of platelets with collagen or convulxin ( a selective GPVI agonist ) resulted in PI3K dependent , and aggregation independent , Ser ( 473 ) and Thr ( 308 ) phosphorylation of PKBalpha , which results in PKB activation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Studies in rodent cells suggest that atypical PKC ( aPKC ) isoforms ( zeta , lamda , and iota ) and PKB , and their upstream activators , PI3K and 3 phosphoinositide dependent protein kinase 1 ( PDK 1 ) , play important roles in insulin stimulated glucose transport . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Endotoxin administration also induced expression of total p 38 and p44 / 42 mitogen activated protein kinases ( MAPK ) in neutrophils , as well as phosphatidyl inositol 3 kinase ( PI3K ) and its downstream target protein kinase B ( PKB ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Although plasma insulin had reached 98 % of its final concentration within 10 min , insulin receptor kinase ( IRK ) activity , p 85 associated with insulin receptor substrate 1 ( IRS 1 ) , IRS 1 associated phosphatidylinositol 3 kinase ( PI3K ) activity , and Thr ( 308 ) protein kinase B ( PKB ) phosphorylation in the muscle biopsies at 20 min had reached only 60 , 48 , 34 and 47 % respectively of those at 180 min . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Recently , we have shown that FSH impacts the IGF 1 pathway via stimulation of the PI3K cascade leading to phosphorylation of protein kinase B ( PKB ) / Akt and the PKB related kinase , Sgk . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
CD 28 costimulation mediates down regulation of p27kip1 and cell cycle progression by activation of the PI3K / PKB signaling pathway in primary human T cells . ^^^ Ligation of TCR / CD3 , but not CD 28 alone , resulted in activation of MEK targets extracellular signal related kinase 1 / 2 , whereas ligation of CD 28 alone was sufficient for activation of PI3K target protein kinase B ( PKB ; c Akt ) . ^^^ Thus , inactivation of the PI3K PKB target GSK 3 could substitute for CD 28 but not for CD 3 signals . ^^^ These results show that the PI3K PKB pathway links CD 28 to cell cycle progression and suggest that p 27 ( kip 1 ) integrates mitogenic MEK and PI3K dependent signals from TCR and CD 28 in primary T lymphocytes . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
AFX like Forkhead transcription factors , which are controlled by phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) signaling , are involved in regulating cell cycle progression and cell death . ^^^ As for the activity of the Forkheads , we also show that protein levels of p 130 are controlled by endogenous PI3K / PKB signaling upon cell cycle reentry . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Insulin stimulates glucose transport and certain other metabolic processes by activating atypical PKC isoforms ( lambda , zeta , iota ) and protein kinase B ( PKB ) through increases in D 3 polyphosphoinositides derived from the action of PI3K . ^^^ Presently , we found that insulin signaling to insulin receptor substrate 1 dependent PI3K , PKB , and PKC lambda , and downstream processes , glucose transport and activation of ERK , were enhanced in skeletal muscles and adipocytes of mice in which the ubiquitous conventional diacylglycerol sensitive PKC isoform , PKC alpha , was knocked out by homologous recombination . ^^^ We conclude that 1 ) PKC alpha is not required for insulin stimulated glucose transport , and 2 ) PKC alpha is activated by insulin at least partly independently of PI3K , and largely serves as a physiological feedback inhibitor of insulin signaling to the insulin receptor substrate 1 / PI3K / PKB / PKC lambda / zeta / iota complex and dependent metabolic processes . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PURPOSE : Phosphatidylinositol 3 ' kinase ( PI3K ) and Akt / protein kinase B ( PKB ) allow for escape from apoptosis in various human cancer cells . ^^^ We postulated that 2 ( 4 morpholinyl ) 8 phenyl chromone ( LY 294002 ) , a PI3K inhibitor , should inactivate Akt / PKB , consequently inhibiting cell proliferation and inducing apoptosis in vitro and in vivo . ^^^ CONCLUSIONS : PI3K Akt / PKB plays an important role in colon cancer development and progression by helping to promote cell growth and allowing cells to escape apoptosis . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Stimulation of the phosphatidylinositol 3 kinase ( PI3K ) protein kinase B ( PKB ) signal transduction pathway has been linked to the neuromodulatory action of ANG 2 in the brain neurons of the spontaneously hypertensive rat ( Yang H and Raizada MK . ^^^ The present study was designed to test the hypothesis that the PI3K PKB signaling cascade activates an ANG 2 mediated neuritogenic action by stimulating cellular growth associated protein 43 ( GAP 43 ) and neurite extension in Wistar Kyoto rat brain neurons . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Activity of the downstream PI3K effector protein kinase B ( PKB ) was higher in SqCas than in AdCas and was correlated with PIK3CA copy number ( r = 0 . 75 ) , suggesting that these copy number increases contribute to activation of PI3K signaling in SqCas of the lung . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
BACKGROUND : Akt / protein kinase B ( PKB ) , which is included in phosphatidyl inositol 3 OH kinase ( PI3K ) signaling , controls many intracellular processes , such as the suppression of apoptosis and the promotion of the cell cycle . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
These findings provide a novel link between CD 28 and an important apoptosis pathway in vivo , and demonstrate that PI3K / PKB signaling prevents apoptosis by inhibiting DISC assembly . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The protein kinase B ( PKB ) / Akt family of serine kinases is rapidly activated following agonist induced stimulation of phosphoinositide 3 kinase ( PI3K ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Recently , links between cAMP , phosphatidylinositol 3 kinase ( PI3K ) and protein kinase B ( PKB ) signaling pathways in thyroid and granulosa cells have been observed . ^^^ The aim of this study was to investigate the possible role of the PI3K / PKB pathway in FSH regulation of Sertoli cell function . ^^^ These increments in P PKB levels were not observed in the presence of two PI3K inhibitors , wortmannin and Ly 294002 . ^^^ Taken together , these results indicate that FSH increases P PKB levels in a PI3K dependent and PKA independent manner in rat Sertoli cells . ^^^ Altogether , these results suggest a meaningful role of the PI3K / PKB pathway in the mechanism of action of FSH in rat Sertoli cells . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
However , we could also show that downstream of phosphoinositide 3 kinase ( PI3K ) , protein kinase B ( PKB ) acted as a previously unknown , potent inhibitor of integrin function and mediator of the disruptive effects of c erbB 2 on adhesion and morphogenesis . ^^^ In addition , the PI3K dependent mTOR / S6 kinase pathway was shown to mediate c erbB 2 induced inhibition of adhesion ( but not spreading ) independently of PKB . ^^^ These results demonstrate a major novel role for PI3K and PKB in regulation of integrin function . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In the biopsies , insulin receptor kinase ( IRK ) activity , insulin receptor substrate ( IRS ) 1 associated phosphatidylinositol 3 kinase ( PI3K ) activity , Ser ( 473 ) and Thr ( 308 ) phosphorylation of protein kinase B ( PKB ) , and protein expression of IRS 1 , IRS 2 , phosphoinositol dependent kinase 1 ( PDK 1 ) , PKB , and GLUT 4 were determined . ^^^ IRK and PI3K activities were not altered by troglitazone , but PKB Ser ( 473 ) phosphorylation was enhanced compared with pretreatment and placebo at the clamp insulin level ( 138 + / 36 vs . 77 + / 16 and 55 + / 13 internal standard units ; both P < 0 . 05 ) and with pretreatment at the basal level ( 31 + / 9 vs . 14 + / 4 internal standard units ; P < 0 . 05 ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The serine / threonine kinase protein kinase B ( PKB / c Akt ) acts downstream of the lipid kinase phosphoinositide 3 kinase ( PI3K ) and functions as an essential mediator in many growth factor induced cellular responses such as cell cycle regulation , cell survival and transcriptional regulation . ^^^ PI3K activation generates 3 ' phosphorylated phosphatidylinositol lipids ( PtdIns3P ) and PKB activation requires PtdIns3P dependent membrane translocation and phosphorylation by upstream kinases . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The serine / threonine protein kinase B ( PKB ) / Akt is a phosphoinositide 3 kinase ( PI3K ) effector that is thought to play an important roll in a wide variety of cellular events . ^^^ A 1 h incubation of neuronal cultures with tetrodotoxin ( TTX ) , the PI3K inhibitor wortmannin , the NMDA receptor antagonist MK 801 or removal of extracellular calcium significantly reduced basal levels of phospho ( Ser 473 ) PKB , indicating that activity dependent glutamate release maintains PKB activation through an NMDA receptor PI3K pathway . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
L1 ) , and FoxO1a ( FKHR ) represent important physiological targets of phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) signaling . ^^^ Overexpression or conditional activation of FoxO factors is able to antagonize many responses to constitutive PI3K / PKB activation including its effect on cellular proliferation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Because insulin exerts a number of its biologic activities via the phosphatidylinositol 3 kinase and protein kinase B ( PI3K / PKB ) signaling pathway , it was the aim of the present study to investigate the role of the PI3K / PKB pathway in the expression of the PAI 1 gene and to identify the insulin responsive promoter sequences . ^^^ Thus , the insulin dependent activation of the PAI 1 gene expression can be mediated via the PI3K / PKB pathway and the transcription factor HIF 1 binding to the HREs in the PAI 1 gene promoter . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Increase of cAMP inhibited Ras activation and phosphorylation of mitogen induced extracellular kinase ( MEK ) downstream targets extracellular signal related kinase 1 / 2 ( ERK1 / 2 ) and phosphatidylinositol 3 kinase ( PI3K ) downstream target protein kinase B ( PKB ; c Akt ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The suppression of various tumor biomarkers including growth factor receptor tyrosine kinases , cytokine receptor kinases , PI3K , phosphatases , ras , raf , MAPK cascades , N 10 FB , 1 10 B kinase , PKA , PKB , PKC , c jun , c fos , c myc , cdks , cyclins , and related transducing proteins by tea polyphenols has been studied in our laboratory and others . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Likewise , HU 210 stimulated the PI3K downstream targets protein kinase B ( PKB ) , as shown by its phosphorylation in Thr 308 and Ser 473 residues , and Raf 1 . ^^^ Moreover , betagamma subunit release mimicked ERK and PI3K / PKB activation , suggesting that activation of class IB PI3K mediates cannabinoid action . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Role of the Raf / MEK / ERK and the PI3K / Akt ( PKB ) pathways in fibroblast senescence . ^^^ We have previously demonstrated that collagenase expression is under positive regulation by the transcription factor FKHRL 1 and that this transcription factor is under negative regulation by the phosphatidylinositol 3 kinase ( PI3K ) / Akt ( PKB ) pathway . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : The effects of LY 294002 , a PI3K inhibitor , on cell growth were examined to elucidate the role of the PI3K Akt / protein kinase B ( PKB ) pathway in colon cancer . ^^^ CONCLUSIONS : Our findings suggest that the silencing of the PIK3CG gene plays an important role in inhibiting the PI3K Akt / PKB signaling system responsible for tumorigenesis and the progression of colorectal cancers . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Relevance of mitogen activated protein kinase ( MAPK ) and phosphotidylinositol 3 kinase / protein kinase B ( PI3K / PKB ) pathways to induction of apoptosis by curcumin in breast cells . ^^^ The PI3K inhibitor LY 294002 ( 50 microM ) inhibited PKB phosphorylation in both cells lines , but only induced apoptosis in the MDA MB 468 line . ^^^ These results suggest that while curcumin has several different molecular targets within the MAPK and PI3K / PKB signalling pathways that could contribute to inhibition of proliferation and induction of apoptosis , inhibition of basal activity of Akt / PKB , but not ERK , may facilitate apoptosis in the tumour cell line . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Transient transfection with the constitutively active PI3K in HERG expressing HEK 293 cells slightly increased ( approximately 7 % ) 1 ( HERG ) while a dominant negative PI3K significantly reduced 1 ( HERG ) ( approximately 25 % ) relative to results in vehicle transfected cells . 1 ( HERG ) was approximately 35 % greater in cells transfected with the constitutively activated PKB ( caPKB ) , whereas it was approximately 47 % smaller in cells transfected with dominant negative PKB ( dnPKB ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In this review , the role of a signal transduction pathway comprised of the lipid kinase , phosphatidylinositol 3 kinase ( PI3K ) , and the serine / threonine kinase , Akt ( or PKB ) , in chemotherapeutic resistance will be explored . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Protein kinase B ( PKB ) is the expression product of a proto oncongen ( c akt ) , which is involved in the signaling pathways initiated by some growth factors and mediated by phosphoinositide 3 kinase ( PI3K ) . ^^^ PKB is a direct target of PI3K . ^^^ The lipid second messengers , PI 3 , 4 P 2 and PI 3 , 4 , 5 P 3 produced by PI3K , can bind to the AH / PH domain of PKB and of PDK ( phosphoinositide dependent protein kinase ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
We present here data indicating that phosphatidylinositol 3 kinase ( PI3K ) is required for NCAM mediated neurite outgrowth from PC 12 E2 cells and from cerebellar and dopaminergic neurones in primary culture , and that the thr / ser kinase Akt / protein kinase B ( PKB ) is phosphorylated downstream of PI3K after stimulation with C 3 . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
They further suggest that downstream effectors of PI3K , including PKB and p 70 S6 kinase , in part mediate cell adhesion , spreading , and microspike formation induced by galectin 8 . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
A 24 h long insulin treatment desensitized the phosphoinositide 3 kinase ( PI3K ) / protein kinase B ( PKB ) and p42 / p44 MAPK pathways toward a second stimulation with insulin or insulin like growth factor 1 and led to decreased insulin induced glucose uptake . ^^^ In summary , ( 1 ) PI3K mediates insulin induced reduction of IRS 1 by phosphorylating it while a PI3K / mTOR pathway controls insulin induced reduction of IRS 2 , ( 2 ) in L 6 cells , IRS 2 is the major adapter molecule linking the insulin receptor to activation of PKB and MAPK , ( 3 ) the mechanism of IRS 1 / 2 down regulation is different in L 6 cells compared with 3T3 L 1 adipocytes . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
ANCA IgG activated PI 3 kinase generating PIP ( 3 ) , activated protein kinase B ( PKB ) , and p 21 ( ras ) ; activation of each mediator was inhibited with pertussis toxin , but PI3K and PKB were not activated by ANCA IgG F ( ab ' ) ( 2 ) fragments . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The ability of deltaRaf 1 : ER * to repress Bim ( EL ) expression required the ERK pathway but was independent of the PI3K > PDK > PKB pathway . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
This was accompanied by reduced expression of IRS 2 and attenuated insulin stimulated recruitment of PI3K to IRS 1 and IRS 2 , yet insulin stimulated PKB activity and phosphorylation of eNOS at Ser 1177 were unaffected . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Survival signals can also be elicited by the activation of phosphatidylinositol 3 kinase ( PI3K ) and protein kinase B ( PKB ) , which in turn inactivates forkhead transcription factors , known to be potent regulators of apoptosis . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PLCgamma 1 directly activates protein kinase C ( PKC ) isozymes ; PI3K stimulates protein kinase B ( PKB , also known as Akt ) , which ultimately activates PKCs and other proteins . ^^^ Western blotting revealed that ( 1 ) PLCgamma 1 is present from E 13 to E 18 but drops off precipitously to negligible levels on the day of birth and thereafter , and ( 2 ) PI3K , PKB ( Akt ) , and several PKC isozymes are expressed from E 13 onward through adult life . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Prolonged exposure to high concentrations of insulin resulted in a desensitized PI3K PKB route . ^^^ The insulin induced inhibition of ERK1 / 2 phosphorylation was also diminished when the PI3K PKB route was desensitized . ^^^ We conclude that PI3K is an important integrator of insulin signalling in Neuro2a cells as it regulates activation of PKB and inhibition of ERK1 / 2 , and is sensitive to the duration of the insulin stimulus . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Growth factor binding events to receptor tyrosine kinases result in activation of phosphatidylinositol 3 kinase ( PI3K ) , and activated PI3K generates the membrane bound second messengers phosphatidylinositol 3 , 4 diphosphate [ PI ( 3 , 4 ) P 2 ] and PI ( 3 , 4 , 5 ) P 3 , which mediate membrane translocation of the phosphoinositide dependent kinase 1 ( PDK 1 ) and protein kinase B ( PKB , also known as Akt ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
We examined here the involvement of the phosphoinositide 3 kinase ( PI3K ) / PKB pathway in the mechanism of action of cannabinoids in human prostate epithelial PC 3 cells . ^^^ The stimulation of PKB induced by cannabinoids was blocked by the two cannabinoid receptor antagonists , SR 141716 and SR 144528 , and by the PI3K inhibitor LY 294002 . ^^^ These results indicate that activation of cannabinoid receptors in PC 3 cells stimulate the PI3K / PKB pathway . ^^^ These results point to a sequential connection between cannabinoid receptors / PI3K / PKB pathway and Raf 1 / Erk in prostate PC 3 cells . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Selective involvement of the PI3K / PKB / bad pathway in retinal cell death . ^^^ The phosphoinositide 3 kinase ( PI3K ) / protein kinase B ( PKB ) / Bad signal transduction pathway is engaged in the control of apoptosis in many different cell types , particularly through phosphorylation of the Bcl 2 family protein Bad . ^^^ Cell death induced by either the PI3K inhibitor LY 294002 , or the general kinase inhibitor 2 aminopurine , were followed by PKB dephosphorylation , but PKB was not modulated during cell death induced by the protein synthesis inhibitor anisomycin . ^^^ The data suggest that Bad may be engaged in survival pathways in the immature retina , but pathways other than PI3K / PKB / Bad , and phosphorylation sites other than Ser 112 and Ser 136 in the Bad protein control cell survival in retinal tissue . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Multiple roles of the PI3K / PKB ( Akt ) pathway in cell cycle progression . ^^^ As its role in tumor progression emerges , the PI3K / PKB ( Akt ) pathway presents an appealing cancer therapeutic target . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The molecular targets of this lipid kinase have been the subject of extensive research , and many functional effects of PI3K activation are thought to be mediated by the serine threonine kinase protein kinase B ( PKB / c akt ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Platelet Derived Growth Factor ( PDGF ) functions upstream of PI3K , while Protein Kinase B ( PKB ) , a downstream effector of PI3K activity , localizes to the leading edge of migrating mesendodermal cells . ^^^ In the absence of PI3K activity , PKB localization and cell polarization are strongly reduced in mesendodermal cells and are followed by slower but still highly coordinated and directed movements of these cells . ^^^ CONCLUSIONS : We have identified a novel role of a signaling pathway comprised of PDGF , PI3K , and PKB in the control of morphogenetic cell movements during gastrulation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
After the cells were treated with specific inhibitor of PI3K ( LY 294002 ) or transfected with antisense cDNA of PKB ( AS PKB ) , all of the above phenotypes were attenuated , and they could not be significantly stimulated by insulin , indicating that the insulin effect on metastatic potential was mediated by PI3K and PKB . ^^^ The upregulation of cell surface SLe ( 10 ) and alpha 1 , 3 fucosyltransferase 7 ( alpha 1 , 3 Fuc T 7 , enzyme for SLe ( 10 ) synthesis ) was also mediated by PI3K and PKB , since LY 294002 and AS PKB also reduced the expressions of SLe ( 10 ) and alpha 1 , 3 FucT 7 , and attenuated the response to insulin . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The aim of the present study was to determine the participation of mitogen activated protein kinase ( MAPK ) and phosphatidyl inositol 3 kinase / protein kinase B ( PI3K / PKB ) pathways in bFGF regulation of Sertoli cell function . ^^^ MAPK kinase inhibitors U 0126 ( U ) and PD 98059 ( PD ) and a PI3K inhibitor wortmannin ( W ) were able to block the stimulatory effects of bFGF on phosphorylated MAPK and PKB levels respectively . ^^^ The participation of MAPK and PI3K / PKB signaling pathways in the regulation by bFGF of two well known Sertoli cell differentiated functions , lactate and transferrin production , was next explored . ^^^ The participation of MAPK and PI3K / PKB signaling pathways in the regulation by bFGF of glucose uptake and lactate dehydrogenase ( LDH ) activity was also analysed . ^^^ In summary , the results demonstrated that bFGF stimulates MAPK and PI3K / PKB dependent pathways in rat Sertoli cells . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Transfection of vectors encoding the p 110 catalytic subunit of PI3K or constitutively active protein kinase B ( PKB ) stimulated GK mRNA and protein expression . ^^^ The transfection of GK promoter constructs together with expression vectors for p 110 or constitutively active PKB revealed that the GK promoter region 87 / 80 mediates the response to PI3K / PKB . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Intraperitoneal administration of TAT Deltap 85 caused time dependent transduction into blood leukocytes , and inhibited activated phosphorylation of protein kinase B ( PKB ) , a downstream target of PI3K , in lung tissues in mice receiving intranasal FMLP . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Wortmannin , a specific phosphatidylinositol 3 kinase ( PI3K ) inhibitor , significantly inhibited serine / threonine kinase Akt / protein kinase B ( PKB ) phosphorylation and reduced HBVSMC migration into the wound edge following VEGF ( 165 ) stimulation ( P < 0 . 05 ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In all , HRG stimulated glucose transport in muscle cells by activation of a pathway that requires PI3K , PDK 1 , and PKCzeta , but not PKB , and that shows cross talk with the MAPK pathway . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PKCzeta appears to mediate the PI3K effect on insulin internalization in a Rab 5 dependent manner , whereas PKB directs intracellular sorting toward intact insulin release . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
DU 145 ( androgen unresponsive prostate carcinoma cells ) and LNCaP ( androgen responsive prostate carcinoma cells ) and , employing immunoblot analysis , we evaluated the effect of epigallocatechin 3 gallate ( EGCG ) , the major polyphenol present in green tea and theaflavins ( TF ) , the major polyphenol present in black tea on phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) and mitogen activated protein kinase ( MAPK ) pathways . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Recently , the ability of trophic factors to promote survival have been attributed , at least in part , to the phosphatidylinositide 3 ' OH kinase ( PI3K ) / Protein Kinase B ( PKB , also named Akt ) cascade . ^^^ Several targets of the PI3K / PKB signaling pathway have been identified that may underlie the ability of this regulatory cascade to promote cell survival . ^^^ PKB possesses a N terminal Pleckstrin Homology ( PH ) domain that binds specifically and with high affinity to PtIns ( 3 , 4 , 5 ) P ( 3 ) and PtIns ( 3 , 4 ) P ( 2 ) , the PI3K second messengers . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
We have recently presented evidence that FSH can stimulate the phosphatidylinositol 3 kinase / protein kinase B ( PI3K / PKB ) pathway in 20 day old Sertoli cells . ^^^ At the same time , it was proposed that in 8 day old Sertoli cells the effects of FSH on phosphorylated PKB ( P PKB ) levels can be explained by a combination of increased secretion of endogenous IGF 1 , decreased IGF binding protein 3 ( IGFBP 3 ) production , and a synergistic action of FSH on IGF 1 dependent PI3K activation . ^^^ The next set of experiments intended to analyze the relevance of a PI3K / PKB pathway to two biological responses of Sertoli cells to FSH and IGF 1 . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
OBJECTIVE : Insulin responsive adipogenic signaling molecules include insulin receptor substrates ( IRS ) 1 and 2 , phosphoinositide 3 kinase ( PI3K ) , and protein kinase B ( PKB ; also known as Akt ) . ^^^ Since p 70 S6K is an insulin responsive kinase downstream of PI3K and PKB , its potential role in adipogenic insulin signaling was investigated . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Protein kinase B ( PKB ) / Akt and its upstream signal transducer , phosphatidylinosito 3 kinase ( PI3K ) play an essential role in control of transcription and translation , which impact cell growth , survival , and metabolism . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
It is proposed that cAMP induced Ntcp translocation involves the activation of the PI3K / PKCzeta signaling pathway followed by the activation of the PI3K / PKB signaling pathway . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In rat hepatocytes , activation of phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) , protein kinase C ( PKC ) and protein phosphatase 1 ( PP 1 ) has been shown to be involved in the GLP 1 induced stimulation of glycogen synthase . ^^^ We first explored the effect of GLP 1 , compared with that of insulin , on the activation of PI3K , PKB , p70s6 kinase ( p70s6k ) and p44 / 42 mitogen activated protein kinases ( MAPKs ) and the action of specific inhibitors of these kinases on the insulin and GLP 1 induced increment in glycogen synthase a activity . ^^^ The study showed that GLP 1 , like insulin , activated PI3K / PKB , p70s6k and p44 / 42 . ^^^ It was concluded that activation of PI3K / PKB and MAPKs is required for the GLP 1 induced increment in glycogen synthase a activity , while PKC , although apparently participating , does not seem to play an essential role ; unlike in insulin signaling , p70s6k , PP 1 and PP 2A do not seem to be needed in the action of GLP 1 upon glycogen synthase a activity in rat muscle . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In the present study , we investigated the role of protein kinase B ( PKB ) ( also known as Akt ) , a general downstream mediator of survival signals through the PI3K cascade , in regulating CVB 3 replication and virus induced apoptosis in a well established HeLa cell model . ^^^ We have demonstrated that CVB 3 infection leads to phosphorylation of PKB / Akt on both Ser 473 and Thr 308 residues through a PI3K dependent mechanism . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Here we demonstrate that increased level of PI3K protein in dexamethasone resistant cells correlates with partial decrease in expression of down stream target of PI3K protein kinase B ( PKB ) . ^^^ We suggest that decreased level of PKB in combination with an activation of PI3K / STAT3 signaling in the melanoma cells after prolonged dexamethasone treatment may be one of the mechanisms of different sensitivity of these cells to hormonal drugs and damaging agents . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
IGF 1 acts in an autocrine and / or paracrine manner to promote glucose utilization , using phosphatidylinositol 3 kinase ( PI3K ) / Akt , also known as protein kinase B ( PKB ) / glycogen synthase kinase 3beta ( GSK3beta ) pathways similar to insulin signaling in peripheral tissues . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Since the phosphoinositide 3 OH kinase ( PI3K ) / protein kinase B ( PKB ) signaling pathway has been shown to prevent apoptosis in various cell types , it was of interest to determine if this pathway could protect against apoptosis induced by act D . ^^^ Inhibition of PI3K / PKB significantly increased act D induced apoptosis . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
AIM : To examine the regulatory effects of angiotensin 2 ( Ang 2 ) on the phosphorylation of 4E binding protein 1 ( 4E BP 1 ) and p 70 S6 kinase in cultured vascular smooth muscle cells ( VSMC ) , and the contribution of phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) signaling pathway in this process . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Insulin receptor substrates ( IRSs ) 1 and 2 are postulated to control the activation of phosphatidylinositol 3 kinase ( PI3K ) dependent signaling factors , namely , atypical protein kinase C ( aPKC ) and protein kinase B ( PKB ) / Akt , which mediate metabolic effects of insulin . ^^^ Presumably , IRS 2 or other PI3K activators account for the normal activation of aPKC in liver and PKB in adipocytes of IRS 1 knockout mice . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
These data demonstrate that ionizing radiation induces VEGF ligand independent but VEGF receptor dependent PKB / Akt activation in endothelial cells and that PI3K / Akt pathway activation by radiation occurs in a differential cell type and receptor dependent pattern . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PI3K stimulates glucose transport through increases in PI 3 , 4 , 5 ( PO ( 4 ) ) ( 3 ) ( PIP ( 3 ) ) , which activates atypical protein kinase C ( aPKC ) and protein kinase B ( PKB / Akt ) . ^^^ Relative to adipocytes of lean women , insulin stimulated [ ( 3 ) H ] 2 deoxyglucose uptake and activation of insulin receptor substrate 1 / PI3K and aPKCs , but not PKB , were diminished in adipocytes of obese women . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Using specific inhibitors , we found that the apoptotic effect of GnRH a is mediated by c Jun NH 2 terminal kinase ( JNK ) and inhibited by the phosphatidylinositol 3 ' kinase ( PI3K ) protein kinase B ( PKB ) pathway . ^^^ Concomitantly , GnRH a reduces the activity of the PI3K PKB pathway , which results in the dephosphorylation of PKB mainly in the nucleus . ^^^ Our results support a potential use of GnRH a for the treatment of advanced prostate cancer and suggest that the outcome of this treatment can be amplified by using PI3K PKB inhibitors . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Activation of phosphatidylinositol 3 kinase protein kinase B ( PI3K PKB ) induced by 17beta estradiol in endometrial carcinoma cell ( Ishikawa ) ] . ^^^ However , the signal transduction of estrogen involving phosphatidylinositol 3 kinase protein kinase B ( PI3K PKB ) is not clear in endometrial carcinoma . ^^^ Our purpose was to study if PI3K PKB signaling pathway could be activated rapidly by 17beta E ( 2 ) through non nuclear action and also , whether PI3K inhibitor , LY 294002 , could inhibit such non nuclear action of 17beta E ( 2 ) in endometrial carcinoma cell line Ishikawa . ^^^ PI3K inhibitor , LY 294002 could inhibit the activation of PKB induced by 17beta E ( 2 ) in Ishikawa cells . ^^^ CONCLUSIONS : 17beta estradiol , by non nuclear action , can activate promptly PI3K PKB signaling pathway in endometrial carcinoma cell line , Ishikawa . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Furthermore , transfection of mouse hepatocytes with PTP1B siRNA downregulated p85alpha protein expression and enhanced insulin induced PKB phosphorylation . ^^^ Treatment of mouse hepatocytes with p85alpha antisense oligonucleotide caused a reduction of p85alpha and an increase in p50alpha and p55alpha isoforms and enhanced insulin stimulated PKB activation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
This mTOR activation is mediated through phosphatidylinositol 3 kinase ( PI3K ) Akt / protein kinase B ( PKB ) signaling cascade because T 3 induced Akt / PKB phosphorylation more rapidly than that of mTOR , and these T 3 dependent phosphorylations were blocked by both PI3K inhibitors and by expression of a dominant negative PI3K ( Deltap85alpha ) . ^^^ The liganded TR induces the activation of PI3K and Akt / PKB , leading to the nuclear translocation of the latter , which subsequently phosphorylates nuclear mTOR . ^^^ The rapid activation of PI3K Akt / PKB mTOR p 70 ( S6K ) cascade by T 3 provides a new molecular mechanism for thyroid hormone action . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Because activation of PKB can require insulin receptor substrate proteins ( IRS 1 and IRS 2 ) and phosphatidylinositol 3 kinase ( PI3K ) , it is of interest to determine whether the activity of Foxo 1 is also regulated by heterotrimeric G protein coupled receptors ( GPCRs ) with IRS 1 or 2 , PI3K , or PKB signaling potential . ^^^ Indeed , studies of beta cells have demonstrated that activation of a GPCR for the blood glucose lowering hormone GLP 1 leads to major alterations of IRS 2 , PI3K , and PKB activity . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Treatment of HUVEC with FGF 2 resulted in dose and time dependent activation of the extracellular regulated kinase ( ERK ) , with moderate effects on phosphoinositol 3 kinase ( PI3K ) and protein kinase B ( PKB ) , also known as AKT , but no effects on glycogen synthase kinase 3 ( GSK3beta ) activity . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Separate measurements of NO production by fluorescence of 2 , 3 diaminonaphthalene ( DAN ) noted that selective inhibitors of phosphatidylinositol 3 kinase ( PI3K ) and protein kinase B ( PKB ) inhibited insulin stimulated NO production , whereas these inhibitors alone did not alter NO production or acetylcholine stimulated NO production . ^^^ Insulin stimulated NO production by endothelium is an acute calcium independent effect mediated via the PI3K PKB signalling pathway . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The triggering of chemokine receptors by their ligands resulted in the activation of phosphatidylinositide 3 kinase ( PI3K ) / protein kinase B ( PKB ) , inhibitory NF kappaB ( IkappaBalpha ) RhoA and extracellular signal regulated protein kinase 1 / 2 ( ERK1 / 2 ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PURPOSE : To identify whether inhibition of phosphatidylinositol 3 kinase ( PI3K ) causes apoptosis through inhibition of protein kinase B ( PKB ) , implicating PKB as an important therapeutic target in prostate cancer . ^^^ RESULTS : Treatment of LNCaP cells with the PI3K inhibitor , LY 294002 , caused inactivation of PKB , growth arrest , and apoptosis . ^^^ Cells expressing constitutively active PKB were protected from apoptosis induced by LY 294002 , but not from the G 1 growth arrest induced by PI3K inhibition . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Insulin signaling through protein kinase Akt / protein kinase B ( PKB ) , a downstream element of the phosphatidylinositol 3 kinase ( PI3K ) pathway , regulates diverse cellular functions including metabolic pathways , apoptosis , mitogenesis , and membrane trafficking . ^^^ These data suggest that WNK 1 is a physiologically relevant target of insulin signaling through PI3K and Akt / PKB and functions as a negative regulator of insulin stimulated mitogenesis . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Glucose dependent insulinotropic polypeptide ( GIP ) stimulation of pancreatic beta cell survival is dependent upon phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) signaling , inactivation of the forkhead transcription factor Foxo 1 , and down regulation of bax expression . ^^^ Treatment of INS 1 ( 832 / 13 ) beta cells with GIP resulted in concentration dependent activation of the phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) / Foxo1 signaling module . ^^^ The results show that PI3K / PKB / Foxo1 signaling mediates GIP suppression of bax gene expression and that this module is a key pathway by which GIP regulates beta cell apoptosis in vivo . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Consistent with a role of PI3K in the R 18 effect , R 18 induced phosphorylation of a protein cloned from the vakt oncogene of retrovirus AKT 8 ( Akt also called protein kinase B , PKB ) at Ser 473 and glycogen synthase 3beta ( GSK3beta ) at Ser 9 , but not extracellular signal regulated kinase 1 / 2 ( ERK1 / 2 ) . ^^^ These results suggest that 14 3 3s inhibits cardiomyocytes hypertrophy through regulation of the PI3K / PKB / GSK3beta and NFAT pathway . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
This event was marked by the induction of several protein kinases such as phosphatidylinositol 3 kinase ( PI3K ) , phosphorylated protein kinase B ( PKB ; also known as Akt ) , p 21 activated kinase 2 ( PAK 2 ) , and their downstream effector ( ERK ) as well as an increase in PKB intrinsic activity . ^^^ PI3K , phospho ( p ) PKB , and PAK were co localized to the site of apical ES in the seminiferous epithelium of the rat testis in immunohistochemistry studies . ^^^ Furthermore , PI3K also co localized with p PKB to the same site in the epithelium as determined by fluorescence microscopy , consistent with their localization at the ES . ^^^ Interestingly , the event of germ cell loss induced by AF 2364 in vivo was also associated with induction of PI3K , p PKB , PAK 2 , and p ERK as well as a surge in intrinsic PKB activity . ^^^ In summary , these results illustrate that Sertoli germ cell anchoring junction dynamics in the testis are regulated , at least in part , via the beta 1 integrin / PI3K / PKB / ERK signaling pathway . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The mammalian signalling pathway involving class 1 PI3K ( phosphoinositide 3 kinase ) , PTEN ( phosphatidylinositol 3 phosphatase ) and PKB ( protein kinase B ) / c Akt has roles in multiple processes , including cell proliferation and apoptosis . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
FoxO factors are regulated by insulin mediated activation of PI3K ( phosphoinositide 3 kinase ) PKB ( protein kinase B ) signalling . ^^^ Activation of PI3K PKB signalling results in the phosphorylation of FoxO factors on three conserved phosphorylation motifs , which are essential for the translocation of FoxO factors from the nucleus to the cytosol . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Treatment of CHO IR cells with H2O2 increased the phosphorylation of extracellular signal regulated kinases 1 and 2 ( ERK1 / 2 ) , protein kinase B ( PKB ) , and glycogen synthase kinase 3beta while enhancing tyrosine phosphorylation of the IR beta subunit and the p 85 subunit of phosphatidylinositol 3 kinase ( PI3K ) . ^^^ However , pharmacological inhibition of Src family PTK by 4 amino 5 ( 4 chlorophenyl ) 7 ( tert butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ( PP 2 ) almost completely blocked H2O2 stimulated phosphorylation of the p 85 subunit of PI3K , ERK1 / 2 , and PKB . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
We show that HPV 16 E 7 increases the level of phosphorylation of PKB in response to serum stimulation , by a mechanism independent of downregulation of PTEN phosphatase , a known inhibitor of the PI3K ( PI 3 kinase ) pathway . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
While expression of PKB , GSK 3 and glycogen synthase was not reduced by dexamethasone treatment , expression of the p85alpha subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) was increased . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Ras initiates phosphatidyl inositol 3 kinase ( PI3K ) / PKB mediated signalling pathways in untransformed human peripheral blood T lymphocytes . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
We have investigated , in isolated rat adipocytes , the changes caused by GLP 1 , Ex 4 and Ex 9 compared with those provoked by insulin or glucagon , upon the activity of phosphatidylinositol 3 kinase ( PI3K ) , protein kinase B ( PKB ) , p42 / 44 MAP kinases ( MAPKs ) and p70s6 kinase ( p70s6k ) , and the participation of these kinases and protein kinase C ( PKC ) in their action upon 2 deoxy d glucose uptake , lipolysis and lipogenesis . ^^^ In normal rat adipocytes , GLP 1 and both exendins share with insulin an increasing action upon the activity of all kinases studied ( except PKB ) , PI3K , p 44 and p 42 MAPKs and possibly PKC , all being required for their stimulating effect upon glucose uptake . ^^^ In cells from STZ rats the magnitude of the above parameters was , in general , comparable to that in normal animals , with some exceptions : basal PI3K activity and lipogenesis were higher , GLP 1 , Ex 4 and Ex 9 failed to modify basal lipogenesis but increased PKB activity , insulin failed to affect the activity of MAPKs and the insulin induced glucose uptake was impaired . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Here we show that insulin activates phosphatidylinositol 3 kinase ( PI3K ) and protein kinase B ( PKB ) , and results in an induction of long term depression ( LTD ) in hippocampal CA 1 neurones . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Here we show that low levels of Ras activity stimulate extracellular signal regulated kinase kinase ( MEK ) p42 / 44 MAPK ( extracellular signal regulated kinase [ ERK ] ) signaling , whereas high levels of Ras activity stimulate additional Pi 3 kinase ( Pi3K ) protein kinase B ( PKB ) signaling , each accounting for approximately 50 % of the potentiation during long term potentiation ( LTP ) . ^^^ In the presence of neuromodulator agonists , neural activity also stimulates the Ras Pi3K PKB pathway that drives GluR 1 into synapses . ^^^ Correspondingly , Ras MEK ERK activity in sleeping animals is sufficient to deliver GluR2L into synapses , while additional increased Ras Pi3K PKB activity in awake animals delivers GluR 1 into synapses . ^^^ Thus , state dependent Ras signaling , which specifies downstream MEK ERK and Pi3K PKB pathways , differentially control GluR2L and GluR 1 dependent synaptic plasticity . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The ability of IGF 1 to activate the pro survival PKB pathway in ATCs was inhibited by LY 294002 , indicating the importance of PI3K in the response of ATCs to IGF 1 . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Blocking the PI3K / PKB pathway in tumor cells . ^^^ A substantial number of experimental and epidemiological studies support an important role for the phosphatidylinositol 3 kinase ( PI3K ) / protein kinase B ( PKB ) pathway in the biology of human cancers . ^^^ This article focuses on the current knowledge of the alterations of the PI3K / PKB pathway in cancer cells and ongoing drug discovery efforts to therapeutically target it . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Insulin regulates glucose transport by activating insulin receptor substrate 1 ( IRS 1 ) dependent phosphatidylinositol 3 kinase ( PI3K ) which , via increases in PI 3 , 4 , 5 triphosphate ( PIP ( 3 ) ) , activates atypical protein kinase C ( aPKC ) and protein kinase B ( PKB / Akt ) . ^^^ In most cases , defective muscle aPKC / PKB activation reflects both impaired activation of IRS 1 / PI3K , the upstream activator of aPKC and PKB in muscle and , in the case of aPKC , poor responsiveness to PIP ( 3 ) , the lipid product of PI3K . ^^^ On the other hand , the defective activation of IRS 1 / PI3K and PKB , as seen in diabetic liver , undoubtedly and importantly contributes to increases in hepatic glucose output . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The lipid kinase phosphoinositide 3 OH kinase ( PI3K ) and its downstream target Akt , also known as protein kinase B ( PKB ) , are crucial effectors in oncogenic signaling induced by various receptor tyrosine kinases . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Wortmannin and LY 29400 , two PI 3 K inhibitors , suppressed the potentiating effects of MMP 2 and preincubation with MMP 2 enhanced the thrombin induced association of the p85alpha PI 3 K subunit with the cytoskeleton and increased the phosphorylation of PKB . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The increase appears to be mediated by activation of PI3K PKB / Akt and / or PI3K mTOR as well as extracellular signal regulated kinase 1 / 2 . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
PURPOSE : To identify whether inhibition of phosphatidylinositol 3 kinase ( PI3K ) causes increased radiosensitivity through inhibition of protein kinase B ( PKB ) , implicating PKB as an important therapeutic target in prostate cancer . ^^^ The expression of constitutively activated PKB blocked apoptosis induced by combination of PI3K inhibition and radiation and prevented radiosensitization by LY 294002 . ^^^ CONCLUSION : These data indicate that PI3K inhibition increases sensitivity of prostate cancer cell lines to ionizing radiation through inactivation of PKB . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The phosphatidylinositol 3 kinase ( PI3K ) / Akt ( protein kinase B , PKB ) signaling pathway plays a critical role in cell growth and survival . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Nonetheless , we present three lines of evidence that inhibition of PI3K / PKB signaling is not required for induction of apoptosis by indole 3 carbinol . ^^^ CONCLUSIONS : Our results show that inhibition of PI3K / PKB signaling by indole 3 carbinol or LY 294002 is not directly correlated with induction of apoptosis in several breast or prostate cell lines . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Neuroanatomical studies of brains from schizophrenic patients report evidence for neuronal dystrophy , while in genetic studies in schizophrenia there is evidence for mutations in growth factors and the downstream enzymes phosphatidylinositide 3 kinase ( PI3K ) and protein kinase B ( PKB ) . ^^^ Since the PI3K PKB pathway is involved in cellular growth and proliferation , reduced activity of this cascade in schizophrenia could at least partly explain the neuronal dystrophy . ^^^ Risk factors for schizophrenia , such as corticosteroids and cannabis , suppress the activity of the PI3K PKB pathway . ^^^ Reduced activity of the PI3K PKB pathway makes the brain more susceptible to virus infections , anoxia , and obstetric complications ( recognized risk factors for schizophrenia ) , whereas a diminution of growth factor levels towards the end of puberty could contribute to an increase in schizophrenia symptoms observed around that time . ^^^ On the other hand , constitutive ( over ) activation of the PI3K PKB pathway increases cancer risk . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Glc HSA induced E selectin expression was suppressed by the phosphatidylinositol 3 kinase ( PI3K ) inhibitors wortmannin and LY 294002 , the protein kinase B ( PKB ) inhibitor ML 9 , the IkappaB kinase ( IKK ) inhibitor BAY 117082 , and the Jun N terminal kinase ( JNK ) inhibitor SP 600125 , On the other hand , the protein kinase C inhibitors calphostin C and H 7 did not suppress Glc HSA induced E selectin expression . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Thus , as the phosphoinositide 3 OH kinase ( PI3K ) / protein kinase B ( PKB ) signaling pathway has been shown to prevent apoptosis in various cell types , we investigated whether this pathway could protect cells against endostatin induced apoptosis . ^^^ It was found that the inhibition of PI3K / PKB significantly increased endostatin induced apoptosis , and that endostatininduced cell death is physiologically linked to PKB mediated cell survival through caspase 8 . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The oncogenic serine / threonine kinase Akt ( also known as PKB ) is a downstream effector of phosphatidylinositol 3 kinase ( PI3K ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
One of the best characterized targets of PI3K lipid products is the serine / threonine protein kinase Akt ( protein kinase B , PKB ) . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Our previous results revealed a new pathway in which amino acids permit insulin induced activation of the protein kinase B ( PKB ) / mTOR pathway in freshly isolated adipocytes when phosphatidylinositol 3 kinase ( PI3K ) is inhibited . ^^^ Moreover , amino acids require the presence of glucose to phosphorylate PKB and to partially rescue glucose transport in a PI3K independent manner . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The up regulation and phosphorylation of PKB by tryptase could be abolished by either phosphoinositol 3 kinase ( PI3K ) inhibitor ( LY 294002 ) at 10 microM or antisense PKB cDNA transfection . ^^^ These results indicate that tryptase can activate PI3K PKB pathway and enhance IL 8 expression . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Taken together , these experiments show that the enhanced phosphorylation of Akt / PKB by OA is dependent on PI3K and suggest that this signaling event may be important for the regulation of OA induced VSMC proliferation . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
In addition , we found that inhibition of p 53 by NIC 1 mainly occurs through mammalian target of rapamycin ( mTOR ) using phosphatidylinositol 3 kinase ( PI3K ) Akt / protein kinase B ( PKB ) pathway as the mTOR inhibitor , rapamycin treatment abrogated NIC 1 inhibition of p 53 and reversed the chemoresistance . ^^^ These results establish that Notch 1 signaling confers chemoresistance by inhibiting p 53 pathway through mTOR dependent PI3K Akt / PKB pathway and imply that p 53 status perhaps is an important determinant in combination therapeutic strategies , which use mTOR inhibitors and chemotherapy . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Treatment of the cells with pharmacological inhibitors of PI3K , wortmannin and 2 ( 4 morpholinyl ) 8 phenyl 4H 1 benzopyran 4 1 ( LY 294002 ) , similarly inhibits FasL induced apoptosis and Akt / PKB phosphorylation , indicating that PI3K is an upstream mediator of Akt / PKB and is involved in Fas mediated cell death . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Despite the absence of the pleckstrin homology domain , activation of the three human isoforms is , like PKB , dependent upon the phosphatidylinositol 3 ' kinase ( PI3K ) pathway that is induced by growth factors and mitogens . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
This inhibition of PKB / Akt activation was because of the impaired interaction between insulin receptor substrate 1 and the p85alpha subunit of phosphatidylinositol 3 kinase . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
BMOV induced PKB phosphorylation was associated with an increased level of tyrosine phosphorylation of the IRbeta subunit , IGF 1Rbeta subunit , IRS 1 , and p85alpha subunit of PI 3 kinase . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The detection of mRNAs for insulin receptor ( IR ) A and IRB ; insulin receptor substrate ( IRS ) 1 and IRS 2 ; phosphoinositide 3 kinase ( PI3K ) catalytic subunits p110alpha , p110beta , PI3KC2alpha , and PI3KC2gamma ; phosphoinositide dependent protein kinase 1 ; protein kinase B ( PKB ) alpha , PKBbeta , and PKBgamma in the beta cell population suggests the presence of a functional insulin signaling cascade in human beta cells . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
To date , activation of PI3K has always correlated with its recruitment into complexes containing protein tyrosine kinases ( PTK ) . ^^^ In addition , some of these agonists also stimulate small increases in PI3K activity in anti phosphotyrosine and anti src type PTK antibody directed immunoprecipitates , indicating activation of PI3K via a ' conventional ' PTK mediated mechanism ; these pathways however , play only a minor role in the initial , agonist sensitive production of PtdIns ( 3 , 4 , 5 ) P 3 in myeloid derived cells . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Genistein ( 30 microM ) and tyrphostin 47 ( 50 microM ) , two PTK inhibitors , as well as LY 294002 ( 10 microM ) , a selective PI3K inhibitor , significantly reduced ( p < 0 . 05 ) peak anaphylactic bronchial contraction and facilitated relaxation of the contracted bronchi . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Thus , Bam 32 may function to integrate protein tyrosine kinase ( PTK ) and phosphatidylinositol 3 kinase ( PI3K ) signaling pathways in B cells . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Activation of PI3K by IL 2 was also blocked by tyrphostin A 25 , a selective inhibitor of PTK , suggesting activation of the latter by IL 2 is upstream to PI3K activation . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Among the various oncogenic PTK activated pathways , phosphatidylinositol 3 kinase ( PI3K ) / Akt signaling is the most critical for anchorage independent survival and growth . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
All the inhibitors prevented lysophosphatidylcholine induced AR , indicating the involvement of PKC , PKA , PTK , PI3K , Akt and the ERK pathway . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Because low K intake has been shown to increase the activity of protein tyrosine kinase ( PTK ) , we explored the role of the interaction between PTK and PI3K in the regulation of the SK channel activity . ^^^ We conclude that PI3K is involved in mediating the effect of low K intake on ROMK channel activity in the CCD and that the effect of PI3K on SK channels requires the involvement of PTK and the cytoskeleton . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
The hypoxia stimulated activities of Rac 1 and Cdc 42 could be blocked by the phosphatidylinositol 3 ' kinase ( PI3K ) inhibitor LY 294002 and the protein tyrosine kinase ( PTK ) inhibitor genistein but were not affected by the p38MAPK inhibitor SB 203580 or the MEK 1 inhibitor PD 98059 , suggesting that the hypoxia mediated signals were through PI3K and PTK . ^^^ Our results indicate that PI3K and PTK mediated activations of Rac 1 and Cdc 42 are involved in the hypoxia induced production of angiogenesis promoting factors and tumor suppressors , and suggest that the Rho family GTPases Rac 1 and Cdc 42 may contribute to the hypoxia mediated angiogenesis . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Placing PYK 2 upstream of both PI3K and Akt activation , PYK 2 activated Akt through a PI3K dependent pathway , and either a dominant negative form of Akt or the PI3K inhibitor LY 294002 blocked PYK 2 stimulated NF kappa B dependent transcription . ^^^ These results indicate that PYK 2 can link G protein activation through PI3K , Akt , and 1 kappa B kinase to NF kappa B activation . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Additionally , muscarinic receptors in PFC slices activated PKC and the focal adhesion kinase Pyk 2 ( a potential molecular link between PKC and Src ) in a PI3K dependent manner . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
These results indicate that 1 ) Ca ( 2+ ) influx through NSCC 1 , NSCC 2 , and SOCC plays essential roles in ET 1 induced PYK 2 phosphorylation , 2 ) NSCC 2 and SOCC are stimulated by ET 1 via a PI3K dependent cascade , whereas NSCC 1 is stimulated via a PI3K independent cascade , and 3 ) PI3K is involved in the PYK 2 phosphorylation that depends on Ca ( 2+ ) influx through SOCC and NSCC 2 . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Effects of nonselective cation channels and PI3K on endothelin 1 induced PYK 2 tyrosine phosphorylation in C 6 glioma cells . ^^^ In addition , we examined the effects of phosphoinositide 3 kinase ( PI3K ) on the ET 1 induced NSCCs activation and PYK 2 phosphorylation . ^^^ The PI3K inhibitors blocked the NSCC 2 dependent part of ET 1 induced PYK 2 phosphorylation . ^^^ These results indicate that 1 ) NSCC 2 is stimulated by ET 1 via a PI3K dependent cascade , whereas NSCC 1 is stimulated via a PI3K independent cascade ; 2 ) PI3K seems to be required for the activation of the Ca2+ entry , but not for its maintenance ; 3 ) Ca2+ influx through NSCC 1 and NSCC 2 plays an essential role in ET 1 induced PYK 2 phosphorylation ; and 4 ) PI3K is involved in the ET 1 induced PYK 2 phosphorylation that depends on the Ca2+ influx through NSCC 2 . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
This study is the first demonstration that PLD 2 activation is implicated in Src dependent phosphorylation of Pyk 2 ( Tyr ( 580 ) and Tyr ( 881 ) ) by promoting the complex formation between Pyk 2 and activated Src in PC 12 cells exposed to H ( 2 ) O ( 2 ) , thereby resulting in activation of the survival signaling pathway PI3K / Akt / p70S6K . . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Surprisingly , we found that Plexin B 1 stimulates PI3K Akt through the activation of an intracellular tyrosine kinase cascade that involves the sequential activation of PYK 2 and Src . ^^^ This results in the tyrosine phosphorylation of Plexin B 1 , the rapid recruitment of a multimeric signaling complex that includes PYK 2 , Src , and PI3K to Plexin B 1 and the activation of Akt . ^^^
Interacting proteins: Q14289 and P27986	Pubmed	SVM Score :0.0
Here we demonstrate that PAR 2 can increase PI3K activity through a Galphaq / Ca ( 2+ ) dependent pathway involving PYK 2 and a Src family kinase , while inhibiting PI3K activity through a beta arrestin dependent mechanism , and that beta arrestin 1 can directly associate with and inhibit the catalytic activity of p110alpha . ^^^