| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Coexpression of ( DN ) Rac 1 N 17 and addition of the phosphatidylinositol 3 ' kinase ( PI3K ) inhibitors wortmannin and LY 294002 are in agreement with a tentative model suggesting that , in the signaling pathway from Ha Ras to the cytoskeleton aPKC lambda acts upstream of PI3K and Rac 1 , whereas aPKC zeta functions downstream of PI3K and Rac 1 . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Microinjection of highly specific inhibitors of PI3K or Rac 1 , or treatment with the p70s6k inhibitor rapamycin , impaired cAMP stimulated DNA synthesis , demonstrating that PKA dependent and independent pathways contribute to cAMP mediated mitogenesis . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| The effect of PDGF on H ( 2 ) O ( 2 ) generation was blocked by the PI3K inhibitors LY 294002 and wortmannin or by overexpression of a dominant negative mutant of Rac 1 . ^^^ These results suggest that a product of PI3K is required for PDGF induced production of H ( 2 ) O ( 2 ) in nonphagocytic cells , and that Rac 1 mediates signaling between the PI3K product and the putative NADPH oxidase . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| These results document a specific PI3K > Rac 1 > PAK 1 > MEK > ERK pathway in NK cells that effects lysis . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| As expected , CDC 42 and Rac 1 activation mediated by EGLT can be completely inhibited by PI3K inhibitors , wortmannin and LY 294002 , and the p 85 dominant negative mutant but not by either the phospholipase C inhibitor , or an intracellular Ca ( 2+ ) chilator BAPTA / AM . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Rac 1 and RhoG promote cell survival by the activation of PI3K and Akt , independently of their ability to stimulate JNK and NF kappaB . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here we report that Vav 3 influences phosphoinositide 3 kinase ( PI3K ) function through Rac 1 in that phosphatidylinositol 3 , 4 , 5 trisphosphate ( PIP 3 ) generation was attenuated by loss of Vav 3 or by expression of a dominant negative form of Rac 1 . ^^^ The functional interaction between PI3K and Rac 1 was also demonstrated by increased PI3K activity in the presence of GTP bound Rac 1 . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Phosphoinositide 3 kinase ( PI3K ) , small Rho GTPases , such as Cdc 42 and Rac 1 , and neuronal Wiskott Aldrich syndrome protein ( N WASP ) are key effectors that regulate dynamic changes in the actin cytoskeleton and cell migration . uPA and EGF stimulated chemotaxis , cytoskeletal rearrangements and activation of Cdc 42 , Rac 1 and N WASP were studied in the highly metastatic human breast cancer cell line MDA MB 231 . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| In NK 92 cells , which express DAP 12 but little DAP10 / NKG2D , we now show that Syk acts upstream of PI3K , subsequently leading to the specific signaling of the PI3K > Rac 1 > PAK 1 > mitogen activated protein / extracellular signal regulated kinase ( ERK ) kinase > ERK cascade that we earlier described . ^^^ Inhibition of Syk function by kinase deficient Syk or piceatannol blocked target cell induced PI3K , Rac 1 , PAK 1 , mitogen activated protein / ERK kinase , and ERK activation , perforin movement , as well as NK cytotoxicity , indicating that Syk is upstream of all these signaling events . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Treating hepatoma cells with 2 AAF activated phosphoinositide 3 kinase ( PI3K ) and its downstream effectors Rac 1 , and NAD ( P ) H oxidase . ^^^ Transient transfection assays demonstrated that constitutively activated PI3K and Rac 1 enhanced the activation of the MDR 1 promoter by 2 AAF . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| H Ras and Rac 1 activate PI3K by targeting the GTPase responsive domain . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| S . aureus stimulation causes the recruitment of active Rac 1 and phosphatidylinositol 3 kinase ( PI3K ) to the TLR 2 cytosolic domain . ^^^ A signaling cascade composed of Rac 1 , PI3K and Akt targets nuclear p 65 transactivation independently of 1 kappa B alpha degradation . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Taken together , our data suggest that Zn ( 2+ ) induces stimulation of the JNK signaling pathway through PI3K Rac 1 signals and that the free radical generation may be an important step in the Zn ( 2+ ) induction of the JNK stimulation . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| ERK activation and downstream effectors of PI3K , such as Rac 1 and Cdc 42 , stimulate expression of cyclin D 1 , a key regulator of G ( 1 ) progression in the mammalian cell cycle . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Expression of Gab 1 PI3K m in SK N MC human primitive neuroectodermal tumor cells expressing wild type RET markedly impaired Akt phosphorylation , Rac 1 activation , and lamellipodia formation that were induced by GDNF whereas expression of Gab 1 SHP2 m partially impaired Erk activation . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| In this report , we demonstrate that p85alpha has GAP activity toward Rab 5 , Rab 4 , Cdc 42 , Rac 1 and to a lesser extent Rab 6 , with little GAP activity toward Rab 11 . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| However , H2O2 could be also produced via other pathway , which is independent of Rac 1 or PI3K , because in Rat 2 cells stably transfected with Rac1N17 , H2O2 could be produced by arsenite , which has been shown to be a stimulator of H2O2 production . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Overexpression of ILK was sufficient to induce Rac 1 activation , which was abolish by a PI3K inhibitor , indicating that Rac 1 activity is involved in ILK signaling in a PI3K dependent manner . ^^^ Inhibition of Akt , Rac 1 , or p70S6K1 inhibited the effects of ILK on actin filaments and cell migration , suggesting a regulatory role of the PI3K / Akt / p70S6K1 / Rac1 signaling pathway in response to ILK signaling . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Furthermore , inhibition of PI3K significantly reduced NGF mediated Rac 1 activation , whereas dominant negative Rac 1 abolished the inhibitory signaling to RhoA . ^^^ Taken together , these data indicate that NGF mediated activation of TrkA receptor stimulates PI3K , which in turn increases Rac 1 activity to induce transient RhoA inactivation during the initial phase of neurite outgrowth . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| These results constitute the first report of a Syk > PI3K > Rac 1 > Akt signal cascade controlled by IL 2 that mediates NK cell survival . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Moreover , using constitutively active versions of Akt 1 and Rac 1 , we show that these downstream pathways of PI3K synergize to achieve anchorage independent growth . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| These data suggest that maspin may inhibit cell motility by regulating Rac 1 and subsequently PAK 1 activity , and promote cell adhesion via PI3K / ERK pathways . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Using this assay , we find that Akt and Rac 1 act as downstream effectors of PI3K and function as control points of cellular proliferation and tissue polarity , respectively . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| We also provide evidence for a positive feedback loop involving Rac 1 and PI3K . ^^^ We also propose that this survival function depends on a positive feedback loop involving Rac 1 and PI3K . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| These results suggest that ROS production in growth factor stimulated cells is mediated by the sequential activation of PI3K , beta Pix , and Rac 1 , which then binds to Nox 1 to stimulate its NADPH oxidase activity . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Canonical TLR signaling by parallel pathways involving MyD 88 , IL 1R associated kinase 1 , TNF receptor associated factor 6 , and IkappaB kinase and Rac 1 , PI3K , and Akt critically mediated NO release in chondrocytes stimulated by both CPPD and MSU crystals . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| RA induced activation of Rac 1 is mediated by phosphatidylinositol 3 kinase ( PI3K ) , probably because of phosphorylation of the p 85 regulatory subunit by Src kinases . ^^^ Over expression of constitutively active PI3K or Rac 1 V12 induces neurite outgrowth , activation of mitogen activated protein kinases ( MAPKs ) , and expression of neuronal markers . ^^^ The PI3K inhibitor LY 294002 , or over expression of dominant negative Rac 1 N17 , blocks RA induced neurite outgrowth , activation of MAPKs , and expression of neuronal markers , suggesting that activation of PI3K / Rac1 signaling represents a potential mechanism for regulation of neuronal differentiation in SH SY5Y cells . . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Activities of NAD ( P ) H oxidase activity , phosphatidyl inositol 3 kinase ( PI3K ) , and SHP 1 were assessed by specific assays ; Rac 1 activation was assessed by a pull down assay . ^^^ The effect of SHP 1 was mediated by inhibition of PI3K / Rac1 dependent NAD ( P ) H oxidase activation ( p < 0 . 01 ) ; SHP 1 AS ODN augmented tyrosine phosphorylation of the p 85 regulatory subunit of PI3K ( p < 0 . 05 ) and Rac 1 activation . ^^^ CONCLUSIONS : In HUVEC , SHP 1 counteracts basal and stimulated NAD ( P ) H oxidase activity by negative regulation of PI3K dependent Rac 1 activation ; SHP 1 thus seems to be an important part of endothelial antioxidative defense controlling the activity of the O 2 ( * ) producing NAD ( P ) H oxidase . . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| In this study , we characterized a mouse guanine nucleotide exchange factor ( GEF ) P Rex2b for its regulation by Gbetagamma and PI3K and its role in S1P induced Rac 1 activation and cell migration in ECs . ^^^ Together with the finding that co expression of Gbetagamma and / or PI3K ( AC ) increased the levels of active Rac 1 , we conclude that P Rex2b is a Rac GEF that can be regulated by Gbetagamma and PI3K . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Rac 1 is critically involved in M CSF receptor signaling and mediates survival signaling primarily through PI3K / Akt pathways . ^^^ Constitutively active Rac 1 enhanced OCL survival , which was completely suppressed by phosphatidylinositol 3 ' kinase ( PI3K ) inhibitors , whereas a Mek inhibitor had only partial effect . ^^^ Rac 1 ( DN ) also partially blocked the activation of Akt induced by the overexpressing catalytic subunit of PI3K . ^^^ CONCLUSIONS : Small guanosine triphosphatase ( GTPase ) Rac 1 is critically involved in M CSF receptor signaling and mediates survival signaling of osteoclasts primarily by modulating PI3K / Akt pathways . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| These morphological changes involved actin cytoskeletal rearrangements controlled by PI3K , Rac 1 and ERK1 / 2 activity . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| TLR 2 transmodulates monocyte adhesion and transmigration via Rac 1 and PI3K mediated inside out signaling in response to Porphyromonas gingivalis fimbriae . ^^^ A dominant negative mutant of Rac 1 also inhibited the lipid kinase activity of PI3K suggesting that Rac 1 acts upstream of PI3K in this proadhesive pathway . ^^^ Thus , P . gingivalis fimbriae activate a novel proadhesive pathway in human monocytes , involving TLR 2 , Rac 1 , PI3K , and CD11b / CD18 , which may constitute a mechanistic basis linking P . gingivalis to inflammatory atherosclerotic processes . . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| The hypoxia stimulated activities of Rac 1 and Cdc 42 could be blocked by the phosphatidylinositol 3 ' kinase ( PI3K ) inhibitor LY 294002 and the protein tyrosine kinase ( PTK ) inhibitor genistein but were not affected by the p38MAPK inhibitor SB 203580 or the MEK 1 inhibitor PD 98059 , suggesting that the hypoxia mediated signals were through PI3K and PTK . ^^^ Our results indicate that PI3K and PTK mediated activations of Rac 1 and Cdc 42 are involved in the hypoxia induced production of angiogenesis promoting factors and tumor suppressors , and suggest that the Rho family GTPases Rac 1 and Cdc 42 may contribute to the hypoxia mediated angiogenesis . . ^^^ |
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| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| Furthermore , inhibiting phosphoinositide 3 kinase ( PI3K ) activity prevented Rac 1 and JNK activation as well as collagen 1 induced N cadherin up regulation . ^^^ These data implicate PI3K Rac 1 JNK signaling in collagen 1 induced changes in NMuMG cells . ^^^ In addition , we showed that cord formation and branching in three dimensional culture ( EMT dependent events ) required N cadherin expression and PI3K Rac 1 JNK signaling . . ^^^ |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P63000 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|