| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| In contrast to SHC , phospholipase C gamma 1 , and the p 85 alpha phosphatidylinositol 3 ' kinase subunit , the endogenous GAP product ( p120GAP ) was highly tyrosine phosphorylated only in cells transformed by wild type 5 src . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| In contrast , stable interaction of mT with Shc , a protein thought to be involved upstream of Ras , is dispensable for pp70S6K activation . 250YS , a mutant mT which retains a binding site for PI3K but lacks one for Shc , stimulates pp70S6K to wild type levels . ^^^ In the polyomavirus systems , the latter requires integration of signals from mT involving both Shc and PI3K . . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Angiogenic growth factors and their receptors , such as basic fibroblast growth factor ( bFGF ) and Flg , vascular endothelial growth factor ( VEGF ) and Flk 1 , platelet derived growth factor ( PDGF ) B chain and PDGF beta receptor , and five intracellular signal proteins ( phosphatidylinositol 3 kinase [ PI3K ] , phospholipase C gamma [ PLC gamma ] , C Src , SHC , and mitogen activated protein kinase [ MAPK ] ) were examined by Western blot analysis . ^^^ Tyrosine phosphorylation of PLC gamma , SHC , and MAPK was increased at 48 hours of reperfusion , and tyrosine phosphorylation of PDGF beta receptor and PI3K was increased at 168 hours of reperfusion . ^^^ The resultant activation of signal proteins PLC gamma , SHC , MAPK , PI3K , and PDGF beta receptor may play an important role in ischemia induced retinal changes such as cell proliferation . . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| We show that phosphatidylinositol 3 ' kinase ( PI3K ) and SHC bind to the HRG activated ErbB 3 in myotubes . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Yet insulin is still required for the stimulation of general protein synthesis in the presence of constitutively active PKC zeta and in the absence of IRS 1 , suggesting a requirement for the convergence of the IRS 1 / PI3K / PKC zeta pathway with one or more additional pathways emanating from the IR , e . g . , Shc / SOS / p21Ras / mitogen activated protein kinase . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| The presence of a type 1 ' turn in the BG loop , which is dependent on the presence of a glycine residue at position BG 3 , is indicative of a binding pocket , characteristic of the Src family , SykC and Abl , rather than a binding groove found in PLC gamma 1C , p 85 alpha N and Shc , for example . . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| The SH 2 domain interaction with CD 45 was specific as glutathione S transferase SH 2 fusion proteins from p 85 alpha subunit of phosphatidylinositol 3 kinase and SHC did not bind to CD 45 . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Ligand caused p 120 rasGTPase activating protein ( GAP ) , SHC and the p 85 subunit of phosphatidylinositol 3 ' kinase ( PI3K ) to be associated with both p185c erbB 2 and p180erbB 4 . ^^^ In addition , tyrosine phosphorylation of p 85 PI3K and SHC , but not of GAP or of its associated p 62 and p 190 proteins , was also detected . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Although the mutated receptor had lost its ability to recruit and / or activate known signaling molecules , such as GRB 2 , SHC , GAB 1 , and PI3K , by using a sensitive association kinase assay we found that the mutated receptor can still associate and phosphorylate a approximately 250 kDa protein . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Ligand stimulation of neuronal cells induced the assembly of a large protein complex containing c Ret , Grb 2 , and tyrosine phosphorylated forms of Shc , p 85 ( PI3K ) , the adaptor Gab 2 , and the protein tyrosine phosphatase SHP 2 . ^^^ These results indicate that upon ligand stimulation , at least two distinct protein complexes assemble on phosphorylated Tyr 1062 of c Ret via Shc , one leading to activation of the Ras / Erk pathway through recruitment of Grb2 / Sos and another to the PI3K / Akt pathway through recruitment of Grb2 / Gab2 followed by p 85 ( PI3K ) and SHP 2 . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| These results show that , in addition to Shc and Grb 2 , a PI3K dependent pathway involving Gab 1 and SHP 2 is essential for Ras activation under EGF stimulation . . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Phosphorylated Y 1062 is part of a Ret multiple effector docking site that mediates recruitment of the Shc adapter and of phosphatidylinositol 3 kinase ( PI3K ) . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Upon ligand binding , the IGF 1 receptor phosphorylates tyrosine residues in SHC and insulin receptor substrates ( IRSs ) initiating two main signalling cascades , the MAP kinase and the phosphatidylinositol 3 kinase ( PI3K ) pathways . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| We examined the roles of two major signaling pathways downstream of Shc , the p44 / p42 mitogen activated protein kinase ( extracellular signal related kinase ( Erk ) ) and phosphatidylinositol 3 kinase ( PI3K ) pathways , in promitogenic gene induction and proliferation in the IL 2 dependent T cell line CTLL 2 . ^^^ Using IL 2R mutants and specific pharmacologic inhibitors , we found that the PI3K , but not Erk , pathway is required for maximal induction of c myc , cyclin D 2 , cyclin D 3 , cyclin E , and bcl 10 ( L ) by Shc . ^^^ Thus , in addition to serving a necessary , but insufficient role in Shc mediated promitogenic gene expression , the PI3K pathway contributes to T cell proliferation by potentiating mitogenic signaling by Stat5 . . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| In addition to the EGF receptor , PI3K C2beta ( 2 298 ) also isolated both Shc and Grb 2 from A 431 cell lysates . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Coexistence of activated Shc and PI3K in a macromolecular complex was suggested by the increase in PI3K activity evident in anti Shc immunoprecipitates prepared from urea treated cells . ^^^ Taken together , these data suggest that PI3K may regulate physiological events in the renal medullary cell response to urea stress and that an upstream tyrosine kinase conferring activation of both PI3K and Shc may govern urea signaling in these cells . . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Indeed , three amino acids important for ligand binding in Src SH 3 were replaced in the Slap SH 3 sequence ; Slap SH 3 did not bind to the Src SH 3 partners p85alpha , Shc , and Sam 68 in vitro , and the chimeric tyrosine kinase Slap / SrcK , composed of SlapDeltaC fused to the SH 2 linker kinase sequence of Src , was not regulated in vivo . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Following Met activation , alpha6beta4 is tyrosine phosphorylated and combines with Shc and PI3K , generating an additional signaling platform that potentiates HGF triggered activation of Ras and PI3K dependent pathways . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation of both receptor forms by antibodies against the alpha subunit and against the carboxy terminus of the beta subunit of the IGF IR suggests that the 75 kDa form could be the beta chain truncated at the amino terminus above the alpha beta disulphide bridges . 3 ) The ATA activated IGF IR forms underwent slow dephosphorylation , compared with a rapid dephosphorylation of the IGF 1 activated receptor . 4 ) The insulin receptor substrate 1 / 2 associated PI3K , Shc proteins , and the kinases Akt and Erk1 / 2 , downstream mediators of the antiapoptotic signaling by IGF IR , were activated to a higher extent and for a longer time period by ATA , compared with IGF 1 . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| IRS 1 and Shc , substrates of the IGF IR , are known to mediate IGF IR signaling pathways such as those of mitogen activated protein kinase ( MAPK ) and phosphatidylinositol 3 kinase ( PI3K ) , which are believed to play important roles in some of the IGF IR dependent biological functions . ^^^ While IGF 1 induced activation of IRS 1 , Shc , PI3K , and MAPK pathways was unaffected , IGF 1 inducible beta 1 integrin associated kinase activity and association of Crk with p 130 ( CAS ) were significantly inhibited by RACK 1 overexpression . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| It is also shown that multiple signaling molecules , including PI3K , SHC , ras GAP and CRK L , are tyrosine phosphorylated in Ba / F3 cells that express ETV6 / ARG . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| In these cells , three major p 85 containing complexes were formed after EGF treatment : ErbB 3 p85 , Shc p 85 , and a multimeric Gab 2 Grb2 SHP 2 p85 , which accounted for more than 80 % of total EGF induced PI3K activity ( Kong , M . , C . ^^^ In summary , after EGF stimulation , ErbB 3 , Shc , and Gab 2 are differentially compartmentalized in rat liver , where they associate with and activate PI3K . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Gab 1 then recruits molecules such as SHP 2 , phosphatidylinositol 3 kinase ( PI3K ) , and Shc . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Using BaF 3 cells expressing a truncated Mpl ( T69Mpl ) as a tool to identify non Shc / Ras dependent signaling pathways , we describe here novel mechanisms of TPO induced ERK activation mediated , in part , by phosphoinositide 3 kinase ( PI3K ) . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Consistent with the decrease , IGF 1 induced IRS 1 association with the p 85 subunit of PI3K and activation of PI3K and Akt were reduced in both WT Shc and 3F Shc cells . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Recent evidence suggest that the tyrosine kinase c Src may mediate this proliferative response . c Src can signal through multiple intracellular signaling pathways including ( 1 ) the Shc / Grb2 / ERK2 pathway , ( 2 ) the signal transducers and activators of transcription ( STATs ) , ( 3 ) the focal adhesion kinase ( FAK ) signaling pathway , and ( 4 ) the phosphatidylinositol 3 kinase ( PI3K ) signaling pathway . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Our results point to the involvement of several ErbB specific ligands ( amphiregulin and neuregulin 1 ) and enzymes or adaptor molecules ( PI3K , Src , Shc and Grb 7 ) in the ErbB pathway dysregulation associated with breast cancer . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| It activates phosphorylation of Shc , and p44 / 42 MAPK ( mitogen activated protein kinase ) in the ERK ( extracellular regulated kinase ) signaling pathway ; PI3K ( phosophatidyl inositol 3 kinase ) , AKT / protein kinase B , and p70S6kinase in the phosophatidyl inositol 3 kinase pathway ; and focal adhesion kinase in the adhesion / motility pathway . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| By using this system , we showed that endosomal activation of PDGFR recruits various signaling proteins including Grb 2 , SHC , phospholipase C gamma 1 , and the p85alpha subunit of phosphatidylinositol 3 kinase into endosomes and forms signaling complexes with PDGFR . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| TEL / ARG was heavily phosphorylated on tyrosine residues and was also found to rapidly induce tyrosine phosphorylation of multiple cellular proteins , including rasGAP , CBL , STAT 5 , PI3K , SHP 2 , Dok , and SHC . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Both Shc and the p85alpha subunit of PI 3 kinase are adapter proteins . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| In some contexts , steroid receptors interact directly with c Src and other cytoplasmic signaling molecules , such as Shc , PI3K , and p 130 Cas . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| We have recently demonstrated that PRL also stimulates the insulin receptor substrates / phosphatidylinositol 3 kinase ( IRSs / PI3K ) and SH 2 plekstrin homology domain ( SHC ) / ERK pathways in islets of neonatal rats . ^^^ In conclusion , downstream proteins of the PI3K ( AKT and p70S6K ) and MAPK ( SHC and ERK1 / 2 ) cascades are regulated by PRL signaling in islets from pregnant rats . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| HGF stimulated Gab 1 association with c Met , Grb 2 , SHP 2 , PI3K , Shc , Crk isoforms and CrkL , but not with PLCgamma 1 . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| The interaction between p85alpha SH 3 and BCR / ABL may be intermediated by proteins such as c Cbl , Shc , Grb 2 , and / or Gab 2 . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| A critical step in this process is the phosphorylation of Tyr 397 of FAK , which creates a binding site for Src family kinases , PI3K ( phosphoinositide 3 kinase ) and Shc ( Src homology and collagen homology ) . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| The adaptor protein Gab 2 coordinates the assembly of the IL 3 signalsome comprising Gab 2 , Grb 2 , Shc , SHP 2 and PI3K . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| PymT , a membrane associated scaffold , recruits and activates Src family tyrosine kinases , and , once tyrosine phosphorylated , binds proteins with PTB and SH 2 domains such as ShcA , phosphatidylinositol 3 kinase ( PI3K ) and phospholipase Cgamma 1 ( PLCgamma 1 ) . ^^^ PymT variants unable to bind PI3K and PLCgamma 1 directly induced hemangiomas similarly to wild type , but a mutant unable to bind ShcA was transformation compromised . ^^^ Surprisingly , PymT recruitment of ShcA and Grb 2 correlated with PI3K activation . ^^^ PymT mimics activated receptor tyrosine kinases by forming a ShcA Grb 2 Gab1 complex , thus inducing Gab 1 tyrosine phosphorylation , which itself is associated with PI3K . ^^^ Therefore , PymT activation of ShcA Grb 2 signaling is critical for endothelial transformation , and PymT can stimulate Grb 2 signaling to both the MAP kinase and PI3K pathways . . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation stimulates PI3K and PLCgamma 1 enzymatic activity , and on ShcA creates binding sites for Grb 2 with its associated Sos 1 and Gab 1 . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| The adapter protein ShcA transmits signals from receptor tyrosine kinases via MAPK ( mitogen activated protein kinase ) / ERK ( extracellular signal regulated kinase ) and PI3K ( phosphatidylinositol 3 kinase ) / Akt signaling pathways . ^^^ |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29353 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|