| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In response to binding of platelet derived growth factor ( PDGF ) , the PDGF receptor ( PDGFR ) beta subunit is phosphorylated on tyrosine residues and associates with numerous signal transduction enzymes , including the GTPase activating protein of ras ( GAP ) and phosphatidylinositol 3 kinase ( PI3K ) . ^^^ Like PI3K , GAP associates only with receptors that have been permitted to autophosphorylate , and GAP itself does not require tyrosine phosphate in order to stably associate with the phosphorylated PDGFR . ^^^ In contrast , the F 771 ( phenylalanine at 771 ) mutant bound wild type levels of PI3K , whereas the F 740 and F 751 mutants bound 3 and 23 % , respectively , of the wild type levels of PI3K but wild type levels of GAP . ^^^ The F740 / F751 double mutant associated with wild type levels of GAP , but no detectable PI3K activity , while the F740 / F751 / F771 triple mutant could not bind either GAP or PI3K . ^^^ The in vitro and in vivo associations of GAP and PI3K activity to these PDGFR mutants were indistinguishable . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| The interactions of the phosphotyrosine ( Tyr ( P ) ) containing proteins in basal and insulin stimulated 3T3 L 1 adipocytes with src homology 2 ( SH 2 ) domains from phosphatidylinositol 3 kinase ( PI3K ) , ras GTPase activating protein ( GAP ) , and phospholipase C gamma have been examined . ^^^ The Tyr ( P ) forms of the insulin receptor and its 160 kDa substrate protein ( pp 160 ) associated with fusion proteins containing either or both the SH 2 domains of PI3K , but not with fusion proteins containing the two SH 2 domains of GAP or phospholipase C gamma . ^^^ These results demonstrate a specificity for the association of the Tyr ( P ) form of the insulin receptor and pp 160 with SH 2 domains that parallels the reported effects of insulin on PI3K , GAP , and phospholipase C gamma in vivo . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Interestingly , GAP and p85 / PI3 ' K binding to distinct p145c kit phosphotyrosines is cooperative , enhancing formation of a heterotetrameric signaling complex , which may include different combinations of p 85 alpha and p 85 beta with p 110 , p 112 , and p 116 by interaction with the same tyrosine 721 docking site . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In both M 23 and MM 55 cells , HGF induces association with MET / HGFR and increased tyrosine phosphorylation of the SH 2 domain containing proteins PI3K , GAP and NCK . ^^^ We therefore suggest that the second messenger proteins PI3K , GAP and NCK , and possibly the protein products of the c fos , c jun , junB , junD and c myc genes , are important elements in the HGF induced mitogenic pathway in the normal mouse epithelial cell lines M 23 and MM55 . . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Binding of platelet derived growth factor ( PDGF ) to the PDGF receptor ( PDGFR ) beta subunit triggers receptor tyrosine phosphorylation and the stable association of a number of signal transduction molecules , including phospholipase C gamma ( PLC gamma ) , the GTPase activating protein of ras ( GAP ) , and phosphatidylinositol 3 kinase ( PI3K ) . ^^^ Previous reports have identified three PDGFR tyrosine phosphorylation sites in the kinase insert domain that are important for stable association of GAP and PI3K . ^^^ Two of them , tyrosine ( Y ) 740 , and Y 751 are required for the stable association of PI3K , while Y 771 is required for binding of GAP . ^^^ An F 1009 / F 1021 double mutant selectively failed to bind both PLC gamma and the 64 kDa protein , whereas all of the carboxy terminal mutants bound wild type levels of GAP and PI3K . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In contrast to SHC , phospholipase C gamma 1 , and the p 85 alpha phosphatidylinositol 3 ' kinase subunit , the endogenous GAP product ( p120GAP ) was highly tyrosine phosphorylated only in cells transformed by wild type 5 src . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| The tyrosine phosphorylated receptor associates with numerous SH 2 domain containing proteins which include phospholipase C gamma 1 ( PLC gamma ) , the GTPase activating protein of Ras ( GAP ) , the p 85 subunit of phosphatidylinositol 3 kinase ( PI3K ) , the phosphotyrosine phosphatase Syp , and several other proteins . ^^^ Our previous studies indicated that PI3K and PLC gamma were required for relay of the mitogenic signal of beta PDGFR , whereas GAP and Syp did not appear to be required for this response . ^^^ Focusing on the PLC gamma dependent branch of beta PDGFR signaling , we constructed a series of mutant beta PDGFRs that contained the binding sites for pairs of the receptor associated proteins : PLC gamma and PI3K , PLC gamma and GAP , or PLC gamma and Syp . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Previously , we reported that after insulin treatment of rat HTC cells expressing human insulin receptors , a unique insulin receptor signaling complex was formed that contained the insulin receptor , the p 85 subunit of PI3K , GTPase activating protein ( GAP ) , and p 62 GAP associated protein . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| We have used a transient expression system and mutant platelet derived growth factor ( PDGF ) receptors to study the binding specificities of the Src homology 2 ( SH 2 ) regions of the Ras GTPase activator protein ( GAP ) and the p 85 alpha subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) . ^^^ Fusion proteins containing the central SH 2 SH3 SH 2 region of GAP or the C terminal region of p 85 alpha , which includes two SH 2 domains , bound to PDGF receptors in response to PDGF stimulation . ^^^ Fusion proteins containing single SH 2 domains from either GAP or p 85 alpha did not bind detectably to PDGF receptors in this system , suggesting that two SH 2 domains in a single polypeptide cooperate to raise the affinity of binding . ^^^ The results suggest that the C terminal GAP SH 2 domain specifies binding to Tyr 771 , the C terminal p 85 alpha SH 2 domain binds to either Tyr 740 or Tyr 751 , and each protein ' s N terminal SH 2 domain binds to unidentified phosphorylation sites . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Ligand caused p 120 rasGTPase activating protein ( GAP ) , SHC and the p 85 subunit of phosphatidylinositol 3 ' kinase ( PI3K ) to be associated with both p185c erbB 2 and p180erbB 4 . ^^^ In addition , tyrosine phosphorylation of p 85 PI3K and SHC , but not of GAP or of its associated p 62 and p 190 proteins , was also detected . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Purified amino terminal Src homology 2 ( SH 2 ) domains of GAP , PLCgamma 1 and the p85alpha subunit of PI 3 kinase , as well as the carboxy terminal SH 2 domain of the latter protein and the unique SH 2 domain of Grb 2 , were injected into full grown , stage 6 Xenopus laevis oocytes . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Mutations eliminating activation of phosphatidylinositol 3 kinase ( PI3K ) , phospholipase C gamma ( PLC gamma ) , the GTPase activating protein ( GAP ) , and Syp phosphatase failed to diminish the induction of type 2 Na+ channel alpha subunit mRNA and functional Na+ channel expression by PDGF , as determined by RNase protection assays and whole cell patch clamp recording . ^^^ Mutation of juxtamembrane tyrosines in combination with mutations eliminating activation of PI3K , PLC gamma , GAP , and Syp abolished the induction of type 2 alpha subunit mRNA , suggesting that at least partially redundant signaling mechanisms mediate this induction . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In rat HTC hepatoma cells overexpressing normal human insulin receptors ( HTC IR ) , p 85 regulatory subunit of phosphatidylinositol 3 kinase ( PI3K ) forms signaling complexes containing the insulin receptor , insulin receptor substrate 1 ( IRS 1 ) , guanosine triphosphatase activating protein ( GAP ) and 60 70 kDa phosphotyrosine proteins ( p 60 70 ) . ^^^ Employing antibodies specific to two p 85 isoforms , p85alpha and p85beta , we demonstrate that HTC IR cells express both p 85 isoforms , and these isoforms induce the formation of similar signaling complexes in response to insulin . p 60 70 , present in both alpha p85alpha and alpha p85beta immunoprecipitates , is a GAP associated protein , but is distinct from the p 68 src associated protein in mitosis ( Sam 68 ) by several criteria . ^^^ These data suggest that 1 ) GAP associated protein , but not Sam 68 , is a part of insulin signaling complexes ; and 2 ) p85alpha and p85beta form similar , but distinct , insulin receptor signaling complexes . . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| A receptor mutant lacking all the binding sites for SHP 2 , GAP , PI3K , and PLC gamma fails to activate JNK 1 . ^^^ Receptor mutants with no binding site for either SHP 2 or GAP can fully activate JNK 1 but those which do not bind either PI3K or PLC gamma are unable to induce JNK 1 activation . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Autophosphorylation of the platelet derived growth factor ( PDGF ) receptor triggers intracellular signaling cascades as a result of recruitment of Src homology 2 domain containing enzymes , including phosphatidylinositol 3 kinase ( PI3K ) , the GTPase activating protein of Ras ( GAP ) , the protein tyrosine phosphatase SHP 2 , and phospholipase C gamma 1 ( PLC gamma 1 ) , to specific phosphotyrosine residues . ^^^ These mutants included a kinase deficient receptor and receptors in which various combinations of the tyrosine residues required for the binding of PI3K ( Tyr ( 740 ) and Tyr ( 751 ) ) , GAP ( Tyr ( 771 ) ) , SHP 2 ( Tyr ( 1009 ) ) , or PLC gamma 1 ( Tyr ( 1021 ) ) were mutated to Phe . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Sam 68 is a docking protein linking GAP and PI3K in insulin receptor signaling . ^^^ In fact , PI3K activity was increased in both anti Sam 68 and anti GAP immmunoprecipitates upon insulin stimulation . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| We show that it is a specific PtdIns ( 3 , 4 , 5 ) P3 / PtdIns ( 3 , 4 ) P 2 stimulated Arf 6 GAP both in vitro and in vivo , and both its Arf GAP and Rho GAP domains cooperate in mediating PI3K dependent rearrangements in the cell cytoskeleton and cell shape . . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| We have recently found that Sam 68 is a substrate of the insulin receptor ( IR ) that translocates from the nucleus to the cytoplasm and that Tyr phosphorylated Sam 68 associates with the SH 2 domains of p 85 PI3K and GAP , in vivo and in vitro . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| The expression of several genes physically mapped to 2q11 2q16 and potentially involved in carcinogenesis including Ccnb ( cyclin B 1 ) , Ccnh ( cyclin H ) , Rasa ( Ras GAP ) , Rasgrf 2 , Pi3kr1 ( p85alpha ) , and Il6st ( gp 130 ) was also examined by quantitative real time PCR and immunohistochemistry ( IHC ) across a large bank of PhIP induced SD rat mammary gland carcinomas . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here we provide evidence that TSC1 / 2 is a GAP for the small GTPase Rheb and that insulin mediated Rheb activation is PI3K dependent . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| ARAP 3 is a PI3K and rap regulated GAP for RhoA . ^^^ We found no evidence for direct regulation of ARAP 3 ' s Rho GAP activity by PtdIns ( 3 , 4 , 5 ) P ( 3 ) in vitro , but PI3K activity was required for activation by Rap in a cellular context , suggesting that PtdIns ( 3 , 4 , 5 ) P ( 3 ) dependent translocation of ARAP 3 to the plasma membrane may be required for further activation by Rap . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| The p85alpha subunit of phosphatidylinositol 3 ' kinase contains a BH domain with sequence homology to GTPase activating proteins ( GAPs ) , but has not previously been shown to possess GAP activity . ^^^ In this report , we demonstrate that p85alpha has GAP activity toward Rab 5 , Rab 4 , Cdc 42 , Rac 1 and to a lesser extent Rab 6 , with little GAP activity toward Rab 11 . ^^^ Purified recombinant Rab 5 and p85alpha can bind directly to each other and not surprisingly , the p85alpha encoded GAP activity is present in the BH domain . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate that transcription from the gap junction dependent osteocalcin CxRE is regulated by extracellular signal regulated protein kinase ( ERK ) and phosphatidylinositol 3 kinase ( PI3K ) cascades . ^^^ These data establish that ERK / PI3K signaling is required for the optimal elaboration of transcription from the osteocalcin CxRE , and that disruption of gap junctional communication attenuates the ability of cells to respond to an extracellular cue , presumably by limiting the propagation of second messengers among adjacent cells by connexin 43 gap junctions . . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Results showed that the expression of PLA 2 , PI3K , Ras p 21 , Ras GAP , ERK MAPK , p38MAPK and PKC was significantly increased . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Recent biochemical analysis revealed that tuberous sclerosis complex ( TSC ) , a GTPase activating protein ( GAP ) , deactivates Rheb and that phosphatidylinositol 3 ' kinase ( PI3k ) Akt / PKB kinase pathway activates Rheb through inhibition of the GAP mediated deactivation . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| As the PI3K / Akt signaling pathway has been demonstrated to alter gap junction expression and GJIC , we selectively blocked phosphoinositide 3 kinase ( PI3K ) . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In the present work , we have found that Sam 68 is tyrosine phosphorylated in peripheral blood mononuclear cells ( PBMC ) from HIV infected subjects , leading to the formation of signalling complexes with p 85 the regulatory subunit of PI3K , GAP and STAT 3 , and decreasing its RNA binding capacity . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| ARAP 3 is a dual GAP for RhoA and Arf 6 that is regulated by phosphatidylinositol ( 3 , 4 , 5 ) trisphosphate [ PtdIns ( 3 , 4 , 5 ) P ( 3 ) ] , a product of the phosphoinositide 3 kinase ( PI3K ) signalling pathway . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| We found that the p 85 alpha subunit of PI 3 kinase binds directly to Rab 5 and possesses GTPase activating protein ( GAP ) activity toward Rab 5 . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| We have found that the p 85 alpha subunit of PI3K binds directly to Rab 5 and possesses GTPase activating protein ( GAP ) activity toward Rab 5 . ^^^ The first method is a steady state GAP assay , used to show that the p 85 alpha protein has GAP activity toward Rab 5 . ^^^ The second method is a single turnover GAP assay and measures changes in the catalytic rate of Rab 5 GTP hydrolysis with or without the p 85 alpha protein . . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| The gene for p 85 alpha is at chromosome region 5q13 , perhaps near the gene encoding another receptor associated signal transducing protein , the GTPase activating protein . . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In normal mast cells , Steel induces Kit to autophosphorylate on tyrosine and bind to phosphatidylinositol 3 ' kinase ( PI3K ) and phospholipase C gamma 1 but not detectably to Ras GTPase activating protein . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Mitotic , tyrosine phosphorylated Sam 68 bound selectively to recombinant SH 2 domains with significantly different affinities ( c Src approximately Ras GTPase activating protein > p 85 alpha ( amino terminal ) > Grb 2 > > p 85 alpha ( COOH terminal ) ) . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In rabbit aortic vascular smooth muscle cells ( VSMC ) platelet derived growth factor BB ( PDGF BB ) stimulated the tyrosine phosphorylation of phospholipase C gamma , p 120 GTPase activating protein , and the p 85 alpha subunit of phosphatidylinositol 3 ' kinase only at high concentrations ( 5 25 ng / ml ) . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| We go on to show two lines of evidence that implicate phosphatidylinositol 3 kinase ( PI3K ) as an important component of PDGF induced mesoderm cell spreading . ( 1 ) The fungal metabolite wortmannin , which inhibits signaling by PI3K , blocks mesoderm spreading in response to PDGF AA . ( 2 ) Activation of a series of receptors with specific tyrosine to phenylalanine mutations revealed PDGF induced spreading of mesoderm cells depends on PI3K but not on other signaling molecules that interact with PDGF receptors including phospholipase C gamma , Ras GTPase activating protein , and phosphotyrosine phosphatase SHPTP 2 . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Upon binding of platelet derived growth factor ( PDGF ) , the PDGF beta receptor ( PDGFR ) undergoes autophosphorylation on distinct tyrosine residues and binds several SH 2 domain containing signal relay enzymes , including phosphatidylinositol 3 kinase ( PI3K ) , phospholipase C gamma ( PLC gamma ) , the GTPase activating protein of Ras ( RasGAP ) , and the tyrosine phosphatase SHP 2 . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| We have used a series of PDGF receptor mutants that display a selective impairment of the binding of SH 2 containing proteins ( GTPase activating protein , SHP 2 , phospholipase Cgamma ( PLCgamma ) , or phosphatidylinositol 3 ' kinase ( PI3K ) ) to show that Tyr 1021 , the PLCgamma binding site , is essential for PKD stimulation by PDGF in A 431 cells . ^^^ F 5 , a receptor mutant that lacks all four binding sites for GTPase activating protein , PLCgamma , PI3K , and SHP 2 , fails to activate PKD . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| On ligand induced tyrosine phosphorylation , PDGFR associated with phospholipase C ( PLC ) gamma 1 , Ras GTPase activating protein ( RasGAP ) , and phosphatidylinositol 3 kinase ( PI3K ) but not with the Syp growth factor receptor bound protein 2 Son of Sevenless complex . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Specific phosphatidylinositol 3 kinase ( PI3K ) inhibitors suppressed both LFA 1 activation and Rap1GTP generation , and abrogation of Rap1GTP by retroviral over expression of a specific Rap 1 GTPase activating protein , SPA 1 , totally inhibited the LFA 1 / ICAM 1 mediated cell adhesion . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Mutant receptors lacking binding sites for activation of the PLCgamma , PI3K , SHP 2 , and RasGAP pathways still retain partial ability to induce 64 of these IEGs . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| When phosphorylated , these sites enable the receptor to recruit signaling molecules PI3K or RasGAP , or enhance the receptor ' s kinase activity , respectively . ^^^ Within the first 10 min , PDGF enhanced the receptor ' s kinase activity and initiated recruitment of PI3K and RasGAP . ^^^ After prolonged exposure to PDGF , PI3K binding persisted to approximately 85 % of the amount bound at 10 min , whereas binding of RasGAP and the exogenous kinase activity of the receptor diminished to less than 15 % of the levels displayed at 10 min . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| It is also shown that multiple signaling molecules , including PI3K , SHC , ras GAP and CRK L , are tyrosine phosphorylated in Ba / F3 cells that express ETV6 / ARG . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| As a consequence , red wine abrogates the ligand induced recruitment of betaPDGFR associated signaling molecules ( RasGAP , SHP 2 , PI3K , PLCgamma ) , PDGF dependent downstream events such as Erk activation and induction of immediate early genes , and VSMC proliferation and migration . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| TEL / ARG was heavily phosphorylated on tyrosine residues and was also found to rapidly induce tyrosine phosphorylation of multiple cellular proteins , including rasGAP , CBL , STAT 5 , PI3K , SHP 2 , Dok , and SHC . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Deletion of the binding site for PI3K , but not for Src , RasGAP , SHP 2 , or PLCgamma , completely abolished the anti apoptotic effect . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| Expression of a partially activated A Raf mutant resulted in decreased tyrosine phosphorylation of the PDGFR , specifically on Y 857 ( autophosphorylation site ) and Y 1021 ( phospholipase Cgamma 1 ( PLCgamma 1 ) binding site ) , but not the binding sites for other signalling proteins ( Nck , phosphatidylinositol 3 ' kinase ( PI3K ) , RasGAP , Grb 2 , SHP ) . ^^^ |
|
| Interacting proteins: P20936 and P27986 |
Pubmed |
SVM Score :0.0 |
| In microcystin transformed cells , PI3K , MAPKAPK 2 , Akt , cyclin D 1 and cyclin D 3 in the Akt pathway ; IQGAP 2 , RabGTPase , Rap1GAP , RasGAP , R Ras , Krev 1 and TC 21 of the Ras GTP / GDP protein family ; and A Raf , B Raf and PAK in the Ras / MAPK pathway were all markedly upregulated . ^^^ |
|