| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| In normal mast cells , Steel induces Kit to autophosphorylate on tyrosine and bind to phosphatidylinositol 3 ' kinase ( PI3K ) and phospholipase C gamma 1 but not detectably to Ras GTPase activating protein . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| We show that induction of adhesion is regulated through two independent pathways of phosphatidylinositol 3 kinase ( PI3K ) and phospholipase C gamma 1 ( PLC gamma ) protein kinase C by elimination of autophosphorylation sites required for activation of PI3K and PLC gamma or in combination with downregulation of protein kinase C or wortmannin . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| The tyrosine phosphorylated receptor associates with numerous SH 2 domain containing proteins which include phospholipase C gamma 1 ( PLC gamma ) , the GTPase activating protein of Ras ( GAP ) , the p 85 subunit of phosphatidylinositol 3 kinase ( PI3K ) , the phosphotyrosine phosphatase Syp , and several other proteins . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| In response to binding of PDGF , the alpha subunit stably associated with phospholipase C gamma 1 ( PLC gamma 1 ) , phosphatidylinositol 3 kinase ( PI3K ) , the phosphotyrosine phosphatase Syp , and a 120 kd protein . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| This effect is not specific to PI3K , since aggregation of phospholipase C gamma 1 to the activated bFGF receptor is also decreased by PMA treatment . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Following binding of platelet derived growth factor ( PDGF ) , the PDGF alpha receptor ( alphaPDGFR ) becomes tyrosine phosphorylated and associates with a number of signal transduction molecules , including phospholipase Cgamma 1 ( PLCgamma 1 ) , phosphatidylinositol 3 kinase ( PI3K ) , the phosphotyrosine phosphatase SHP 2 , Grb 2 , and Src . ^^^ Characterization of the F 720 mutant indicated that binding of two proteins , SHP 2 and Grb 2 , was severely impaired , whereas PLCgamma 1 and PI3K associated to wild type levels . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that c kit is present in mature sperm , and its binding to SCF may result in the activation of PLC gamma 1 and PI3K , leading to receptor autophosphorylation , and ultimately may play a role in capacitation and / or the acrosome reaction . . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| These events are preceded by the ligand induced tyrosine phosphorylation of the receptor and its association with SH 2 containing signaling enzymes including Src family members ( Src ) , the phosphotyrosine phosphatase SHP 2 , phosphatidylinositol 3 kinase ( PI3K ) , and phospholipase C gamma 1 ( PLCgamma ) . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| This study shows a direct evidence that the SH 2 SH2 SH 3 region of PLCgamma 1 contributes to the NF kappaB signaling and that MAPK , but not PI3K and PKC , is involved in SH 2 SH2 SH 3 mediated NF kappaB activation in these cells . . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : Previously , the authors showed that epidermal growth factor ( EGF ) stimulates phospholipase Cgamma 1 ( PLCgamma 1 ) and phosphoinositide 3 kinase ( PI3K ) activities in confluent rabbit corneal epithelial cells ( RCECs ) . ^^^ The purpose of this study was to investigate whether PLCgamma 1 activity is upregulated during EGF induced proliferation of RCECs and to determine whether there is any cross talk between PLCgamma 1 and PI3K in these cells . ^^^ Treatment of the cells with the PI3K inhibitors , wortmannin or LY 294002 , caused inhibition of both EGF stimulated cell proliferation and PLCgamma 1 activation . ^^^ The data also show that PLCgamma 1 activation and cell proliferation were inhibited by PI3K inhibitors , suggesting a role for PI3K in EGF stimulated proliferation of corneal epithelial cells . . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| PymT , a membrane associated scaffold , recruits and activates Src family tyrosine kinases , and , once tyrosine phosphorylated , binds proteins with PTB and SH 2 domains such as ShcA , phosphatidylinositol 3 kinase ( PI3K ) and phospholipase Cgamma 1 ( PLCgamma 1 ) . ^^^ PymT variants unable to bind PI3K and PLCgamma 1 directly induced hemangiomas similarly to wild type , but a mutant unable to bind ShcA was transformation compromised . ^^^ |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Early studies established the importance of the activation of the enzymes phospholipase C gamma 1 ( PLC gamma 1 ) and phosphatidylinositol 3 kinase ( PI3K ) , as well as the small molecular weight heterotrimeric guanine nucleotide binding protein ( G protein ) Ras , in this process . ^^^ |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation stimulates PI3K and PLCgamma 1 enzymatic activity , and on ShcA creates binding sites for Grb 2 with its associated Sos 1 and Gab 1 . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Several transducing molecules , including JAK 2 , STAT 5 , MAP kinases , phosphatidylinositol 3 kinase ( PI3K ) , phospholipase C gamma 1 , and PKC are activated by interaction between erythropoietin ( EPO ) and the EPO receptor . ^^^ |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Autophosphorylation of the platelet derived growth factor ( PDGF ) receptor triggers intracellular signaling cascades as a result of recruitment of Src homology 2 domain containing enzymes , including phosphatidylinositol 3 kinase ( PI3K ) , the GTPase activating protein of Ras ( GAP ) , the protein tyrosine phosphatase SHP 2 , and phospholipase C gamma 1 ( PLC gamma 1 ) , to specific phosphotyrosine residues . ^^^ These mutants included a kinase deficient receptor and receptors in which various combinations of the tyrosine residues required for the binding of PI3K ( Tyr ( 740 ) and Tyr ( 751 ) ) , GAP ( Tyr ( 771 ) ) , SHP 2 ( Tyr ( 1009 ) ) , or PLC gamma 1 ( Tyr ( 1021 ) ) were mutated to Phe . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| EGF stimulated signaling by means of PI3K , PLCgamma 1 , and PKC isozymes regulates branching morphogenesis of the fetal mouse submandibular gland . ^^^ Branching morphogenesis of fetal mouse submandibular glands ( SMGs ) partly depends on the epidermal growth factor ( EGF ) receptor that triggers at least three intracellular signaling pathways involving ( 1 ) the mitogen activated protein kinases ERK 1 / 2 , ( 2 ) phospholipase Cgamma 1 ( PLCgamma 1 ) , and ( 3 ) phosphatidylinositol 3 kinase ( PI3K ) . ^^^ PLCgamma 1 directly activates protein kinase C ( PKC ) isozymes ; PI3K stimulates protein kinase B ( PKB , also known as Akt ) , which ultimately activates PKCs and other proteins . ^^^ Here , we report on components of the PLCgamma 1 , PI3K , and PKC families of signaling molecules in fetal SMGs from the 13th day of gestation to postnatal ages . ^^^ Western blotting revealed that ( 1 ) PLCgamma 1 is present from E 13 to E 18 but drops off precipitously to negligible levels on the day of birth and thereafter , and ( 2 ) PI3K , PKB ( Akt ) , and several PKC isozymes are expressed from E 13 onward through adult life . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| In immunohistochemical studies , phosphoinositide 3 kinase ( PI3K ) was detected in the membrane of the cell soma and the immunoreactivity of PI3K ( PI3K IR ) was almost overlapped with MOR IR and PLC gamma 1 IR in the periaqueductal gray matter ( PAG ) that is considered to be one of the most important sites for the expression of MOR mediated antinociception . ^^^ Morphine produced a marked increase in the protein level of membrane bound PLC gamma 1 , and this increase induced by morphine was significantly inhibited by intracerebroventricullar ( i . c . v . ) pretreatment with PI3K inhibitors at the dosage that suppressed the morphine induced supraspinal antinociception . ^^^ It is worthwhile to note that MOR IR was overlapped with p PLC gamma 1 IR in the same cells that also contained PI3K in this region . ^^^ In addition , the morphine induced supraspinal antinociception was suppressed by the blockade of the G beta gamma subunit that can directly activate both PI3K and PLC gamma 1 . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Vav 1 has also been shown to control the activation of phospholipase Cgamma 1 ( PLCgamma 1 ) via both phosphoinositide 3 kinase ( PI3K ) dependent and independent pathways . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| The TCR signaling proteins that are subject to regulation by PI3K include Akt , phospholipase Cgamma 1 ( PLCgamma 1 ) , protein kinase C zeta ( PKC zeta ) , Itk , Tec and Vav , all of which play critical roles in T cell activation . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| In this report , we demonstrate that Raf 1 associated with the SH 2 domain of Fyn ( a member of the Src tyrosine kinase family ) but not with the SH 2 domains of phospholipase C gamma 1 , the p 85 alpha subunit of phosphatidylinositol 3 kinase , and SH 2 containing protein tyrosine phosphatase 2 . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| In contrast to SHC , phospholipase C gamma 1 , and the p 85 alpha phosphatidylinositol 3 ' kinase subunit , the endogenous GAP product ( p120GAP ) was highly tyrosine phosphorylated only in cells transformed by wild type 5 src . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| PI3K activation is not affected in PLC z / HBMEC upon infection , whereas PKC alpha phosphorylation is completely abolished , indicating that PLC gamma 1 is downstream of PI3K . ^^^ Concomitantly , the phosphorylation of PLC gamma 1 is blocked in HBMEC overexpressing a dominant negative form of the p 85 subunit of PI3K but not in HBMEC overexpressing a dominant negative form of PKC alpha . ^^^ Activation of PI3K is associated with the conversion of phosphatidylinositol 4 , 5 bisphosphate ( PIP 2 ) to phosphatidylinositol 1 , 4 , 5 trisphosphate ( PIP 3 ) , which in turn recruits PLC gamma 1 to the cell membrane via its interaction with pleckstrin homology domain of PLC gamma 1 . ^^^ Taken together , these results suggest that E . coli infection of HBMEC induces PLC gamma 1 activation in a PI3K dependent manner to increase Ca2+ levels in HBMEC . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Expression of a partially activated A Raf mutant resulted in decreased tyrosine phosphorylation of the PDGFR , specifically on Y 857 ( autophosphorylation site ) and Y 1021 ( phospholipase Cgamma 1 ( PLCgamma 1 ) binding site ) , but not the binding sites for other signalling proteins ( Nck , phosphatidylinositol 3 ' kinase ( PI3K ) , RasGAP , Grb 2 , SHP ) . ^^^ Activated A Raf expression also altered the activation of PLCgamma 1 , and p 85 associated PI3K . ^^^ Thus , A Raf can regulate PLCgamma 1 signalling via a PDGFR dependent mechanism and may also regulate PI3K signalling via a PDGFR independent mechanism . . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| PI3K inhibitors blocked calcium activation of PLC gamma 1 as well as the induction of keratinocyte differentiation markers involucrin and transglutaminase . ^^^ These data indicate that calcium activates PLC gamma 1 via increased PIP 3 formation mediated by c src and fyn activated PI3K . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| HGF stimulated Gab 1 association with c Met , Grb 2 , SHP 2 , PI3K , Shc , Crk isoforms and CrkL , but not with PLCgamma 1 . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| We identify PLCgamma 1 , PLCgamma 2 , and PI3K as the major signaling mediators downstream of CRACC / EAT 2 implicated in NK cell mediated cytotoxicity . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| EGFR activates PLC gamma 1 directly and activates Akt indirectly through phosphatidylinositol 3 kinase ( PI3K ) . ^^^ Many studies have shown that the PLC gamma 1 pathway and PI3K Akt pathway interact with each other . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Purified amino terminal Src homology 2 ( SH 2 ) domains of GAP , PLCgamma 1 and the p85alpha subunit of PI 3 kinase , as well as the carboxy terminal SH 2 domain of the latter protein and the unique SH 2 domain of Grb 2 , were injected into full grown , stage 6 Xenopus laevis oocytes . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Other downstream effectors of the TCR / CD3 transduction machinery , such as phosphatidylinositol 3 kinase p85alpha , p 59 ( fyn ) , linker for activation of T cells ( LAT ) , and phospholipase C gamma 1 , are not affected . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| Further studies , conducted in mast cells derived from the bone marrow of mice deficient in the p 85 alpha and p 85 beta subunits of PI 3 kinase , also revealed no defects in Fc epsilon RI mediated PLC gamma 1 activation . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| By using this system , we showed that endosomal activation of PDGFR recruits various signaling proteins including Grb 2 , SHC , phospholipase C gamma 1 , and the p85alpha subunit of phosphatidylinositol 3 kinase into endosomes and forms signaling complexes with PDGFR . ^^^ |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27986 and P19174 |
Pubmed |
SVM Score :0.0 |
| NA |
|