| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.70787801 |
| Because p 85 ( PI3K ) is known to bind to the activated c Kit receptor , the possibility that CRKL interacted with c Kit indirectly through p 85 ( PI3K ) was investigated . 0.70787801^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.55009569 |
| All of the mutant KIT and 53 . 8 % ( 7 / 13 ) of wild type KIT were phosphorylated ( activated ) and associated with phosphorylated phosphatidylinositol 3 kinase ( PI3K ) . 0.55009569^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Upregulation of the PI3K / Akt network in AML may be due to several reasons , including FLT 3 , Ras or c Kit mutations . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Binding of c kit ligand , stem cell factor ( SCF ) to c kit receptor ( c kitR ) is known to activate c kitR tyrosine kinase , thereby leading to autophosphorylation of c kitR on tyrosine and to association of c kitR with substrates such as phosphatidylinositol 3 kinase ( PI3K ) . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that c kit is present in mature sperm , and its binding to SCF may result in the activation of PLC gamma 1 and PI3K , leading to receptor autophosphorylation , and ultimately may play a role in capacitation and / or the acrosome reaction . . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| All the events associated with SCF induced cell cycle progression are inhibited by the addition of either a PI3K inhibitor or a mitogen activated protein kinase kinase ( MEK ) inhibitor , indicating that both MEK and PI3K are essential for c kit mediated proliferative response . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| In contrast , these studies demonstrated that the D816V c Kit mutant was constitutively associated with phosphorylated p85PI3K , and , downstream of PI3K , Jnk 1 and Jnk 2 were activated but Akt was not . ^^^ Thus , D816V c Kit maintains the activity of PI3K but not of all signaling pathways activated by wild type c Kit . ^^^ Studies with a PI3K inhibitor and D816V / Y721F c Kit , a mutant incapable of recruiting PI3K , indicate that constitutive activation of PI3K through direct recruitment by D816V c Kit plays a role in factor independent growth of MIHC and is critical for tumorigenicity . . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Consistent with the pharmacologic results , abrogating the binding of the p85alpha subunit of class IA PI 3kinase to c Kit also resulted in inhibition of SCF induced migration on fibronectin . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| In contrast , Erk activation induced by stem cell factor , which activates c Kit in the same cells , is sensitive to Raf inhibition and insensitive to PI3K and PKC inhibitors . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Therefore , it seemed that SCF initiated an anti apoptotic signal starting from its membrane receptor c kit to Bcl 2 family members through PI3K / AKT and other signaling cascades in the oocytes of primordial follicles . . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| In the WBA cell line , in which the c Kit receptor tyrosine kinase is partly responsible for basal PI3K Akt activity , the combination of NVP ADW 742 and imatinib was superior to NVP ADW 742 alone in sensitizing the cells to etoposide . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Several novel target genes of Epo were identified , and the majority were regulated in a PI3K dependent manner , including KIT ( CD 117 ) and CDH 1 ( E cadherin ) . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Our previous analysis of the signal transduction pathway used by the c kit encoded receptor for the stem cell factor ( SCF ) indicated efficient coupling to the type 1 phosphatidylinositol 3 ' kinase ( PI3K ) . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Bacterially expressed SH 2 domains derived from the p 85 alpha subunit of PI 3 kinase bound in vitro to the activated colony stimulating factor 1 receptor and to Kit . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| In normal mast cells , Steel induces Kit to autophosphorylate on tyrosine and bind to phosphatidylinositol 3 ' kinase ( PI3K ) and phospholipase C gamma 1 but not detectably to Ras GTPase activating protein . ^^^ Additionally , we present evidence that Kit tyrosine phosphorylation acts as a switch to promote complex formation with PI3K . ^^^ In mast cells from mice homozygous for the W 42 mutant allele , Kit is not tyrosine phosphorylated and fails to bind PI3K following Steel stimulation . ^^^ In contrast , in the transformed mast cell line P 815 , Kit is constitutively phosphorylated and binds to PI3K in the absence of ligand . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| In distinction to Kit and colony stimulating factor 1R , mutant receptors unable to couple to PI3K and expressed in rodent fibroblasts or in the interleukin 3 dependent cell line Ba / F3 provide a mitogenic signal comparable to wild type receptors . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Taken together these results suggest that coexpression of SCF and Kit may enhance responsiveness to erbB ligands by enhancing activation of the MAPK and PI3K pathways . . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| LY 294002 , which inhibits all classes of PI3Ks , strongly suppressed Kit and FcepsilonRI induced responses in p85alpha / mast cells , revealing the contribution of another PI3K family member ( s ) . ^^^ Impaired kit but not FcepsilonRI initiated mast cell activation in the absence of phosphoinositide 3 kinase p85alpha gene products . ^^^ We have determined the effects of disrupting the p85alpha gene on the responses of mast cells stimulated by the cross linking of Kit and FcepsilonRI , receptors that reflect innate and adaptive responses , respectively . ^^^ The absence of p85alpha gene products partially inhibited Kit ligand / stem cell factor induced secretory granule exocytosis , proliferation , and phosphorylation of the serine / threonine kinase Akt . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| In Y719F , the binding of phosphatidylinositol 3 ' kinase ( PI3K ) to KIT was lost and KIT mediated cell migration and Ca ( 2+ ) mobilization were suppressed by PI3K chemical inhibitors or dominant negative PI3K , suggesting the involvement of Y 719 mediated PI3K pathway in cell migration . ^^^ Taken together , these results indicate that 2 major KIT signaling pathways lead to cell migration , one is Y 567 SFK p 38 MAPK Ca ( 2+ ) influx Erk and the other is Y 719 PI3K Ca ( 2+ ) influx . . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| The SCF protective effect was maintained in 32D KitYF 719 cells in which the PI3K / Akt signaling pathway is abolished due to mutation in Kit docking site for PI3K . ^^^ |
|
| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : These findings show the role of PI3K in KIT ( Asn822Lys ) mediated constitutive activation through the Akt independent downstream signaling pathway of JNK , and also demonstrate the mutant ' s susceptibility to STI 571 , which may therefore have therapeutic potential in CBFL patients with susceptible KIT mutations . . ^^^ |
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| Interacting proteins: P10721 and P27986 |
Pubmed |
SVM Score :0.0 |
| Finally , purified p85alpha / p85beta+ / c kit+ cells had a decrease in proliferation in response to kit ligand ( kitL ) , a growth factor important for controlling HSC function in vivo . ^^^ |
|