| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| We previously showed by M 13 phage display that the Src , Fyn , Lyn , and phosphatidylinositol 3 kinase ( PI3K ) SH 3 domains preferred the same class 1 type core binding sequence , RPLPP psi P . ^^^ These libraries were screened for binding to the Src , Fyn , Lyn , Yes , and PI3K SH 3 domains . ^^^ The amino acids selected in the flanking sequences were similar for Src , Fyn , and Yes SH 3 domains ; however , Lyn and PI3K SH 3 domains showed distinct binding specificities . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| In this report , we demonstrate that Raf 1 associated with the SH 2 domain of Fyn ( a member of the Src tyrosine kinase family ) but not with the SH 2 domains of phospholipase C gamma 1 , the p 85 alpha subunit of phosphatidylinositol 3 kinase , and SH 2 containing protein tyrosine phosphatase 2 . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here we report the synthesis and solution structure of the amino terminal SH 3 domain of GRB 2 and of its more stable Ser 32 mutant . 1H NMR analysis of the complex between the Ser 32 GRB 2 N SH 3 domain and the proline rich peptide VPPPVPPRRR , derived from h Sos , shows that relative to the SH 3 peptide complexes described for PI3K , Fyn and Abl , the proline rich peptide in this complex binds in the opposite orientation . . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| Identification of Src , Fyn , Lyn , PI3K and Abl SH 3 domain ligands using phage display libraries . ^^^ We have employed a novel modification of phage display involving biased libraries to identify peptide ligands of the Src , Fyn , Lyn , PI3K and Abl SH 3 domains . ^^^ The Src SH 3 domain prefers the sequence XXXRPLPPLPXP , Fyn prefers XXXRPLPP ( I / L ) PXX , Lyn prefers RXXRPLPPLPXP , PI3K prefers RXXRPLPPLPP while the Abl SH 3 domain selects phage containing the sequence PPPYPPPP ( I / V ) PXX . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| Both peptides worked equally well in inhibiting the binding of PI 3 kinase activity and p 85 alpha to Fyn SH 3 domain , whereas a control peptide had no effect . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| Mutagenesis studies in COS 7 cells , transiently transfected with CD 8 epsilon , p85alpha , and Fyn cDNAs in various combinations , show that both Tyr 170 and Tyr 181 within the CD 3 epsilon ITAM are required for efficient binding of p85alpha PI 3 kinase . ^^^ Thus , replacement of Tyr 170 by Phe ( Y170F ) , or Tyr 181 by Phe ( Y181F ) significantly reduces binding of p85alpha PI 3 kinase , whereas it does not affect binding of Fyn . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that Jak 3 does not play a significant role in the antigen induced degranulation and phosphorylation of MAPKs , and that WHI P 131 and WHI P 154 inhibit the PI3K pathway by preventing the antigen induced activation of Fyn , thus inhibiting the antigen induced degranulation and phosphorylation of MAPKs in mast cells . . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| The combination of dominant negative src and fyn blocked calcium induced tyrosine phosphorylation of the regulatory subunit of PI3K , p85alpha , and the activity of the catalytic subunit of PI3K . ^^^ These data indicate that calcium activates PLC gamma 1 via increased PIP 3 formation mediated by c src and fyn activated PI3K . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| Other downstream effectors of the TCR / CD3 transduction machinery , such as phosphatidylinositol 3 kinase p85alpha , p 59 ( fyn ) , linker for activation of T cells ( LAT ) , and phospholipase C gamma 1 , are not affected . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| The alpha 7 nicotinic receptor was physically associated with the PI3K p 85 subunit and Fyn . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| This includes the protein tyrosine kinases Src , Hck , and Fyn , the p85alpha regulatory subunit of phosphatidylinositol 3 kinase , phospholipase Cgamma , and the adaptor protein Grb 2 . ^^^ |
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| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06241 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|