| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| The class 2 phosphoinositide 3 kinases ( PI3K ) PI3K C2alpha and PI3K C2beta are two recently identified members of the large PI3K family . ^^^ In this study , we demonstrate that PI3K C2alpha and PI3K C2beta represent two downstream targets of the activated epidermal growth factor ( EGF ) receptor in human carcinoma derived A 431 cells . ^^^ Ligand addition also induced the appearance of a second , more slowly migrating band of PI3K C2alpha and PI3K C2beta immunoreactivity on sodium dodecyl sulfate polyacrylamide gel electrophoresis . ^^^ To examine this interaction in greater detail , PI3K C2beta was chosen for further investigation . ^^^ EGF and platelet derived growth factor also stimulated the association of PI3K C2beta with their respective receptors in other cells , including epithelial cells and fibroblasts . ^^^ |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| Previously we demonstrated that the class 2 phosphoinositide 3 kinase C2beta ( PI3K C2beta ) is rapidly recruited to a phosphotyrosine signaling complex containing the activated receptor for epidermal growth factor ( EGF ) . ^^^ In this study the interaction between PI3K C2beta and the EGF receptor is competitively attenuated by synthetic peptides derived from each of three proline rich motifs present within the N terminal region of the PI3K . ^^^ Further , a series of N terminal PI3K C2beta fragments , truncated prior to each proline rich region , bound the receptor with decreased efficiency . ^^^ Since these findings revealed that the interaction between the EGF receptor and PI3K C2beta is indirect , we sought to identify an adaptor molecule that could mediate their association . ^^^ In addition to the EGF receptor , PI3K C2beta ( 2 298 ) also isolated both Shc and Grb 2 from A 431 cell lysates . ^^^ |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| The activity of nuclear phosphoinositide 3 kinase C2beta ( PI3K C2beta ) was investigated in HL 60 cells induced to differentiate along granulocytic or monocytic lineages . ^^^ A significant increase in the activity of immunoprecipitated PI3K C2beta was observed in the nuclei and nuclear envelopes isolated from all trans retinoic acid ( ATRA ) differentiated cells which was inhibited by the presence of PI3K inhibitor LY 294002 . ^^^ Western blot analysis of the PI3K C2beta immunoprecipitates with anti P Tyr antibody revealed a significant increase in the level of the immunoreactive band corresponding to PI3K C2beta in the nuclei and nuclear envelopes isolated from ATRA differentiated cells . . ^^^ |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| The activity of nuclear phosphoinositide 3 kinase C2beta ( PI3K C2beta ) was investigated in HL 60 cells blocked by aphidicolin at G ( 1 ) / S boundary and allowed to progress synchronously through the cell cycle . ^^^ The activity of immunoprecipitated PI3K C2beta in the nuclei and nuclear envelopes showed peak activity at 8 h after release from the G ( 1 ) / S block , which correlates with G ( 2 ) / M phase of the cell cycle . ^^^ On Western blots , PI3K C2beta revealed a single immunoreactive band of 180 kDa , whereas in the nuclei and nuclear envelopes isolated at 8 h after release , the gel shift of 18 kDa was observed . ^^^ When nuclear envelopes were treated for 20 min with mu calpain in vitro , the similar gel shift and increase in PI3K C2beta activity was observed which was completely inhibited by pretreatment with calpain inhibitor calpeptin . ^^^ The presence of PI3K inhibitor LY 294002 completely abolished the calpain mediated increase in the activity of PI3K C2beta but did not prevent the gel shift . ^^^ |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here , we show that lysophosphatidic acid ( LPA ) stimulates the production of PtdIns 3 P through activation of a class 2 PI3K ( PI3K C2beta ) . ^^^ Both PtdIns 3 P and PI3K C2beta are involved in LPA mediated cell migration . ^^^ This study is the first identification of PtdIns 3 P and PI3K C2beta as downstream effectors in LPA signaling and demonstration of an intracellular role for a class 2 PI3K . ^^^ Defining this novel PI3K C2beta PtdIns 3 P signaling pathway may help clarify the process of cell migration and may shed new light on PI3K mediated intracellular events . . ^^^ |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| However , the mechanism by which PI3K C2beta catalytic activity is regulated currently remains unknown . ^^^ We demonstrate that increased expression of EGFR enhanced its association with PI3K C2beta following stimulation with EGF . ^^^ Deletion of the first proline rich region within the N terminus precluded recruitment of PI3K C2beta to activated EGFR . ^^^ Using an N terminal PI3K C2beta GST fusion protein , clathrin heavy chain was affinity purified from A 431 cell lysates . ^^^ Association of PI3K C2beta with clathrin was confirmed by co immunoprecipitation from cell lysates while intracellular co localisation of PI3K C2beta and clathrin was confirmed by confocal microscopy . ^^^ |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| Investigation of three genes encoding catalytic subunits of Pi3k ( PIK3CA , PIK3CD , and PIK3C2B ) revealed amplification of PIK3C2B ( 1q32 ) in 6 tumors ( 6 % ) . ^^^ |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00750 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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