| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.72500066 |
| Activation of PI3K by glucose dependent insulinotropic polypeptide and glucose was associated with insulin receptor substrate isoforms insulin receptor substrate 2 and growth factor bound 2 associated binder 1 and PI3K isoforms p85alpha , p110alpha , p110beta , and p110gamma . 0.72500066^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| However , the effect of PI3K catalytic subunit p110delta on VEGF mediated signaling is unknown . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The mouse gene encoding the PI3K adapter subunit p85alpha and its splice variants p55alpha and p50alpha was disrupted . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In RAW murine macrophages , p 55 , p85alpha , and p85beta PI3K subunits were present at isolated lipid bodies . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| New responsibilities for the PI3K regulatory subunit p 85 alpha . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| This effect was partially dependent on the PI3K subunits p85alpha and p110gamma . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We report herein the cloning and characterization of p110delta , a novel class 1 PI3K . ^^^ However , distinct biochemical and structural features of p110delta suggest divergent functional / regulatory capacities for this PI3K . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here , the protein kinase activity of the tyrosine kinase linked PI3K , p110delta , is characterized and its functional impact assessed . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Comparison of the genomic structure with that of p110alpha , beta , and gamma demonstrates that the p110delta gene shares its exon structure with p110beta , the most closely related PI3K at the amino acid level . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| However , in this report we demonstrate that inhibition of epidermal growth factor ( EGF ) stimulated PI3K activity by expression of the kinase deficient PI3K p 110 subunit ( p110delta kin ) does not block the lysosomal targeting and degradation of the EGF receptor ( EGFR ) . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In this study , we determined that TC 21 interacts strongly with some ( RalGDS , RGL , RGL2 / Rlf , AF 6 , and the phosphatidylinositol 3 kinase ( PI3K ) catalytic subunit p110delta ) , and weakly with other Ras small middle dotGTP binding proteins . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The phosphoinositide 3 kinase ( PI3K ) catalytic subunit p110delta , the most recently discovered member of the heterodimeric Class IA PI3K family , has been detected uniquely in leukocytes , but not in one member of the leukocyte family : platelets . ^^^ We have utilized p110delta directed Western blotting , RT PCR , PI3K activity assays , and immunoprecipitations of PI3K Class IA subunits p85alpha , p85beta , and p110delta from lysed human platelets , as well as Triton 10 100 insoluble cytoskeletal preparations from resting and thrombin receptor activated platelets . ^^^ We report that p110delta is present in association with p85alpha and p85beta in platelets , both in cytosolic and cytoskeletal fractions . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Western analyses of aortic homogenates revealed the presence of p85alpha , p110alpha , p110beta , and p110delta but not p110gamma PI 3 kinase subunits ; p110delta protein was elevated in aorta of hypertensive rats as compared with sham . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Isoform specific neutralization of PI3K isoforms in breast cancer cell lines ( by PI3K antibody microinjection or a p110delta selective pharmacological inhibitor ) demonstrated that p110delta is the most important class IA PI3K in the regulation of epidermal growth factor driven motility in vitro , controlling the directionality and , to a lesser extent , the speed of migration . ^^^ These results show a nonredundant function of PI3K isoforms downstream of the epidermal growth factor receptor and indicate that the presence of p110delta may confer breast cancer cells with selective migratory capacities . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| On the other hand , the messages of PI3K IIalpha , Vps 34 , and p110delta decreased and that of PI3K IIbeta increased during aging . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Notably , p110alpha was silenced without affecting levels of either the other class 1 ( A ) PI3K catalytic subunits p110beta and p110delta , or the p85alpha regulatory subunit . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Western analyses of resistance artery protein homogenate revealed p85alpha and p110delta subunit protein , with significantly elevated levels of p110delta protein in the DOCA salt compared with sham rats ( 0 . 30+ / 0 . 07 versus 0 . 16+ / 0 . 04 % smooth muscle alpha actin arbitrary units ) . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| An inactivating mutation in the leucocyte specific PI3K isoform p110delta results in impaired TcR dependent proliferation under circumstances where CD 28 co stimulation is blocked or not required . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| As B cell and T cell chemotactic responses were both sensitive to the general PI3K inhibitors , wortmannin ( WMN ) and LY 294002 , we explored whether B cell responses were affected in mice lacking p110delta , a major PI3K isoform in lymphocytes . ^^^ These observations establish that p110gamma and p110delta function in lymphocyte chemotaxis , and show differential roles for PI3K family members in B and T cell migration . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The p85alpha and p110delta subunits of PI3K both participate in anti IgM and mIg / CD19 coligation induced Ca ( 2+ ) flux , although the defects are not as severe as observed after pharmacological inhibition . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| However , pharmacological inhibition of PI3K by wortmannin or deletion of the p110delta catalytic subunit has no effect on Vav activation in response to BCR engagement , suggesting that this mechanism does not operate in vivo . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Using mice deficient in the p110delta subunit of PI3K , we investigated the role of this subunit in LPS ( lipopolysaccharide ) induced B cell responses , which are mediated by Toll like receptor 4 and RP 105 . ^^^ This demonstrates that the p110delta subunit of PI3K is involved in the LPS response in B cells and may represent a link between the innate and the adaptive immune system . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| PI 3 kinase p85alpha , p110alpha , and p110delta mRNA was 1 . 5 to 2 fold higher in the paraventricular nucleus ( PVN ) of spontaneously hypertensive rats ( SHR ) compared with their controls , Wistar Kyoto rats ( WKY ) . ^^^ The increase in p85alpha / p110delta was attenuated in SHR treated with captopril , an angiotensin ( Ang ) converting enzyme inhibitor , from in utero to 6 months of age . ^^^ In summary , increased p85alpha / p110delta expression in the PVN and RVLM is associated with increased PI 3 kinase activity in the SHR . ^^^ Furthermore , normalized PI 3 kinase p85alpha / p110delta expression within the PVN might contribute to the overall effect of captopril , perhaps attributable to a consequent decrease in NE availability . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Of the 8 distinct mammalian isoforms of PI3K , it is the class 1 PI3Ks ( p110alpha , p110beta , p110gamma , and p110delta ) that are responsible for Akt activation . ^^^ Here we show that the p110delta isoform of PI3K is consistently expressed at a high level in blast cells from AML , in contrast to the other class 1 isoforms , the expression of which was very variable among patients . ^^^ IC 87114 , a p110delta selective inhibitor , suppressed both constitutive and Flt 3 stimulated Akt activation in blasts to the same extent as Ly 294002 , an inhibitor of all PI3K isoforms . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The role of PI3K activity in T lymphocyte development is obscure because mice deficient in single PI3K catalytic subunits either die before birth ( p110alpha / and p110beta / ) or lack a significant T cell developmental phenotype ( p110gamma / and p110delta / ) . ^^^ This work demonstrates that PI3K activity is critical for T cell development and depends on the combined function of p110gamma and p110delta . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The non selective PI3K inhibitor LY 294002 ( 20 micromol / L ) and the selective inhibitor of the p110delta catalytic subunit of PI3K IC 87114 ( 20 micromol / L ) caused a fall of basal tone in SHR aorta ( 20 + / 7 and 24 + / 6 % of the initial PE contraction , respectively ) , but did not alter tone in arteries from WKY rats . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Key role of the p110delta isoform of PI3K in B cell antigen and IL 4 receptor signaling : comparative analysis of genetic and pharmacologic interference with p110delta function in B cells . ^^^ Mouse gene targeting studies have documented a central role of the p110delta isoform of phosphoinositide 3 kinase ( PI3K ) in B cell development and function . ^^^ The PI3K dependent component of intracellular calcium mobilization also completely relies on p110delta catalytic activity . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Furthermore , all high risk MDS patients also demonstrated high expression of the Class 1 PI3K p110delta catalytic subunit and a decreased expression of PTEN . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| P110delta phosphoinositide 3 kinase ( PI3K ) plays a pivotal role in the recruitment and activation of certain inflammatory cells . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Rat neutrophils expressed both class IA PI3K subunits ( p 85 , p110alpha , p110beta , and p110delta ) and a class IB PI3K subunit ( p110gamma ) as assessed by a combination of Western blotting and reverse transcription polymerase chain reaction ( RT PCR ) approaches . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Platelets express all type 1 PI3K isoforms , including p110alpha , p110beta , p110delta and p110gamma , with recent evidence suggesting important roles for p110gamma and p110beta in regulating distinct phases of the platelet activation process . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The p110delta isoform of PI3K differentially regulates beta 1 and beta 2 integrin mediated monocyte adhesion and spreading and modulates diapedesis . ^^^ METHODS : The authors employed the PI3K p110delta catalytic subunit specific inhibitor IC 87114 ( 2 microM ) and the broad spectrum PI3K inhibitory agents LY 294002 ( 50 microM ) and wortmannin ( 100 nM ) , to examine the role of PI3Kdelta in monocyte diapedesis through endothelial monolayers and its role in monocyte adhesion and spreading upon carpets of ICAM 1 or VCAM 1 . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The major cytosolic substrate of the insulin receptor is a 185 kDa phosphoprotein ( IRS 1 ) that contains multiple putative attachment sites for the p 85 alpha regulatory subunit of phosphatidylinositol 3 ' kinase ( PI3K ) . ^^^ The latter was found to undergo specific association with the p 85 alpha regulatory subunit of PI3K but not with two other proteins that contain src homology domains . ^^^ As p 85 alpha was not detectably phosphorylated on tyrosine residues and did not appear to interact directly with the insulin receptor , we conclude that tyrosine phosphorylation of pp 185 promotes its association with p 85 alpha and the catalytic subunit of PI3K . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Finally , tryptic peptide maps show that p56lck phosphorylates three tyrosine residues in the p 85 alpha subunit of PI3K and two in p 110 of PI3K . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The in vitro phosphorylation of p 85 alpha or p 110 alpha derived from thrombin stimulated platelets was no different than that of resting platelets , but we confirm that in thrombin receptor stimulated platelets enhanced levels of p 85 alpha and PI3K lipid kinase activity were recovered in antiphosphotyrosine antibody immunoprecipitates . ^^^ These results suggest PI3K alpha can autophosphorylate on serine and threonine , and both p 85 alpha and p 110 alpha are substrates for a constitutively associated protein tyrosine kinase in platelets . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We fused the inter Src homology region 2 of the regulatory p85alpha subunit of PI3K ( iSH 2 ) either to a C terminal sequence of GLUT 4 ( G4c , amino acids 406 509 ) or to this region and the N terminal tail of GLUT 4 ( G4n , amino acids 1 19 ) , resulting in the fusion proteins iSH 2 G4c and G4n iSH 2 G4c , respectively . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| To determine the role of PI3K in glucose homeostasis , we generated mice with a targeted disruption of the gene encoding the p85alpha regulatory subunit of PI3K ( Pik3r1 ; refs 3 5 ) . ^^^ Insulin stimulated PI3K activity associated with insulin receptor substrates ( IRSs ) was mediated via full length p 85 alpha in wild type mice , but via the p 50 alpha alternative splicing isoform of the same gene in Pik3r1 / mice . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Interestingly , a strong association of p85alpha and p110alpha subunits of PI3K with beta catenin is induced in V12Ras expressing keratinocytes , and in vitro binding assays show a direct interaction between beta catenin and p85alpha . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| By using purified preparations we show that nanomolar concentrations of Gbetagamma significantly stimulated lipid kinase activity of phosphatidylinositol 3 kinase ( PI3K ) beta and PI3Kgamma in the presence as well as in the absence of non catalytic subunits such as p85alpha or p 101 . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| LY 294002 , which inhibits all classes of PI3Ks , strongly suppressed Kit and FcepsilonRI induced responses in p85alpha / mast cells , revealing the contribution of another PI3K family member ( s ) . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| There was no difference in the mRNA abundances of IRS 1 , GLUT 4 , p 85 alpha phosphatidylinositol 3 kinase ( p 85 alpha PI3K ) or Rad . ^^^ In control subjects , the diet increased p 85 alpha PI3K ( +146 % ) , insulin receptor ( +100 % ) and Rad ( +40 % ) mRNA concentrations in muscle . ^^^ In Type 2 diabetic patients , the diet increased insulin receptor ( +41 % ) and Rad ( +31 % ) mRNAs but the expression of p 85 alpha PI3K was not modified . ^^^ CONCLUSION / INTERPRETATION : The regulation of the expression of p 85 alpha PI3K is altered during caloric restriction in skeletal muscle of Type 2 diabetic patients . ^^^ Because we have shown in an earlier study that there is also a defective regulation of p 85 alpha PI3K gene expression in response to insulin , these data support the hypothesis that alterations in the regulation of gene expression could be involved in the pathogenesis of Type 2 diabetes . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Dexamethasone induces overexpression of the PI3K subunit p85alpha , which , in turn , competes with the complete PI3K heterodimer for binding at insulin receptor substrate 1 , inhibiting PI3K activation . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Ras activation was inhibited by a Grb 2 dominant negative form ( P49L ) , by PI3K inhibitors , including wortmannin , LY 294002 , the N SH 2 domain of p85alpha PI3K and by the SH 2 domain of Src . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Both transformation of Rat 1 fibroblasts by 5 Src or K Ras and stable transfection for expression of dominant positive , wild type phosphoinositide 3 kinase ( PI3K ) regulatory subunit p 85 alpha constitutively led to stress fiber disruption , cortical actin recruitment , extensive ruffling , and macropinosome formation , as measured by a selective acceleration of fluid phase endocytosis . ^^^ These alterations closely correlated with activation of PI3K and phosphatidylinositol specific phospholipase C ( PI PLC ) , as assayed by 3 phosphoinositide synthesis in situ and in vitro and inositol 1 , 4 , 5 trisphosphate steady state levels , respectively ; they were abolished by stable transfection of 5 Src transformed cells for dominant negative truncated p 85 alpha expression and by pharmacological inhibitors of PI3K and PI PLC , indicating a requirement for both enzymes . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Induction of the Cdc 42 pathway is independent of phosphoinositide 3 kinase ( PI3K ) enzymatic activity , but it is dependent on the p85alpha regulatory subunit of PI3K . ^^^ These observations show the essential role of the PI3K regulatory subunit p85alpha in controlling PDGF receptor induced cytoskeletal changes and cell migration , illustrating a novel signaling pathway that links receptor stimulation at the cell membrane with actin dynamics . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In humans , the Met326Ile missense variant of the p85alpha regulatory subunit of the phosphoinositide 3 kinase ( PI3K ) has been associated with either significant reductions in glucose effectiveness and intravenous glucose tolerance in Caucasians or a significantly higher insulin secretory response in Pima Indians . ^^^ In the present study , we genotyped 1 , 190 Caucasian males to evaluate the impact in vivo of the Met326Ile variant of the p85alpha subunit of PI3K on the acute insulin response , intravenous glucose tolerance , insulin mediated glucose uptake , and the prevalence of type 2 diabetes after 20 years of follow up . ^^^ We conclude that the Met326Ile variant of the p85alpha regulatory subunit of PI3K is likely to be as functionally normal in vivo as in vitro . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Activated PI3K is composed of a catalytic subunit ( p110alpha or beta ) associated with one of a large family of regulatory subunits ( p85alpha , p85beta , p55gamma , p55alpha , and p50alpha ) . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In contrast with previous reports , immunoblots and indirect immunofluorescence failed to detect the p85alpha subunit of the heterodimeric PI3K within VSMC nuclei . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Basal mRNA levels ( determined by reverse transcriptase competitive polymerase chain reaction ) of insulin receptor , insulin receptor substrate 1 , p85alpha phosphatidylinositol 3 kinase ( PI3K ) , p110alphaPI3K , p110betaPI3K , GLUT 4 , glycogen synthase , and sterol regulatory element binding protein 1c ( SREBP 1c ) were similar in muscle of control ( n = 17 ) , type 2 diabetic ( n = 9 ) , type 1 diabetic ( n = 9 ) , and nondiabetic obese ( n = 9 ) subjects . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Moreover , we found that ERalpha binds to the p85alpha regulatory subunit of PI3K in the absence or presence of estradiol in epithelial cells and subsequently activates PI3K / AKT2 , suggesting ERalpha regulation of PI3K / AKT2 through a nontranscriptional and ligand independent mechanism . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Using three independent assays , we demonstrated that the C terminal ( CT ) SH 2 domain , but not the N terminal SH 2 domain , on the PI3K p85alpha subunit displayed discriminative affinity for PIP ( 3 ) . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We have used single strand conformational polymorphism / heteroduplex analysis to demonstrate the presence of somatic mutations in the gene for the p85alpha regulatory subunit of PI3k ( PIK3R1 ) in primary human colon and ovarian tumors and cancer cell lines . ^^^ Expression of a mutant protein with a 23 amino acid deletion leads to constitutive activation of PI3k providing the first direct evidence that p85alpha is a new oncogene involved in human tumorigenesis . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| These results suggest either that p85alpha negatively regulates insulin signaling , or that p85beta , which mediates the major fraction of Class IA PI3K signaling in the absence of p85alpha , is more efficient than p85alpha in mediating insulin responses . ^^^ These results indicate that in addition to their roles in recruiting the catalytic subunit of PI3K to the insulin receptor substrate proteins , both p85alpha and p85beta play negative roles in insulin signaling . . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Total levels of p85alpha subunit of PI3K and Akt were not influenced by plasma NEFA levels either in the basal state or during the glucose clamps . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Mice that lack the p85alpha regulatory subunit of phosphatidylinositol 3 kinase ( PI3K ) are deficient in gastrointestinal and peritoneal mast cells but have dermal mast cells . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation studies demonstrated that , in a ligand dependent manner , raloxifene increased ERalpha associated p85alpha , p110alpha , and PI3K activity . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| These include a decreased IRS 2 pool level , a decrease in PI3K activity and its association with IRS 2 and decreased docking of p85alpha to IRS 2 . ^^^ |
|
| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The decreased activity of the insulin / IGF 1 signaling pathway is indicated by decrease of ( a ) IRS two pool levels ; ( b ) docking of p 85 alpha to IRS 2 ; ( c ) docking of p 85 alpha to p 110 alpha or p 110 beta , and ( d ) IRS 2 associated PI3K activity . ^^^ Our data show that the PI3K activity associated with IRS 1 , the docking of IRS 1 to InR beta and the docking of p 85 alpha to IRS 1 are attenuated in the aged Snell dwarf . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Treatment of BT 474 cells with Herceptin inhibited the constitutive tyrosine phosphorylation of HER 3 and disrupted the basal association of HER 3 with HER 2 and of HER 3 with p85alpha potentially explaining the inhibition of PI3K . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| AT 2 receptor stimulation did not change insulin induced tyrosine phosphorylation of IRS 2 or its association with the p85alpha subunit of PI3K , but led to a significant reduction of insulin induced p85alpha phosphorylation . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Co immunoprecipitation analysis showed a constitutive association between c Src and PI3K , which was enhanced by PTH treatment , suggesting that the cytosolic tyrosine kinase forms an immunocomplex with PI3K probably via the N SH 2 domain of the p85alpha regulatory subunit . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Phosphoinositide 3 kinases ( PI3Ks ) constitute a family of lipid kinases that regulate an array of fundamental cellular responses by neutrophils [ polymorphonuclear leukocytes ( PMN ) ] . p85alpha Gene disrupted mice were used to help accurately identify the physiological role of the PI3K isoform in PMN activation in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^ In terms of targeting strategy , however , the mutation actually expressed a small amount of Ia type ( p85alpha regulated ) PI3K activity ( partially abrogated ) in the mice . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The p85alpha regulatory subunit of class 1 ( A ) phosphoinositide 3 kinases ( PI3K ) is derived from the Pik3r1 gene , which also yields alternatively spliced variants p50alpha and p55alpha . ^^^ Although , IGF 1 stimulated PI3K activity associated with insulin receptor substrates was unaltered in all cell lines , p85alpha null ES cells showed diminished protein kinase B activation despite increased PI3K activity associated with the p85beta subunit . ^^^ However , differentiated ES cells partially lost their ability for compensatory signaling at the level of PI3K , which may explain some of the defects observed in mice with homozygous deletion of the Pik3r1 gene . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In the present paper , we compare and contrast the phenotypes of p 110 delta mutant mice with those of mice that lack p 85 alpha or p 110 gamma , and discuss these in the context of PI3K signalling in B and T cells . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Hormone induced tyrosine phosphorylation of p85alpha , the regulatory subunit of PI3K , as well as the phosphorylation on Thr ( 308 ) of its downstream effector Akt / PKB was evident in enterocytes from 3 month old rats , whereas it was greatly reduced in the cells from 24 month old animals . ^^^ Intracellular Ca ( 2+ ) chelation ( BAPTA AM , 5 microM ) affected the tyrosine phosphorylation of p85alpha and inhibited PTH dependent PI3K activation by 75 % in young rats and completely abolished the enzyme activity in aged animals , demonstrating that Ca ( 2+ ) is required for full activation of PI3K in enterocytes stimulated with PTH . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| To investigate the roles that enzymes play in platelet function in vivo and determine which isoforms are important for particular signaling events , we analyzed platelet function of gene knockout mice deficient in the p85alpha regulatory subunit of heterodimeric class IA PI3K . ^^^ Significant attenuation of CRP induced tyrosine phosphorylation in known PI3K effectors such as Btk , Tec , PKB / Akt , and phospholipase Cgamma 2 were observed with p85alpha / platelets , whereas no alteration was noted in upstream molecules of Syk , LAT , and SLP 76 . ^^^ Considered as a whole , these results provide the first genetic evidence that PI3K p85alpha plays a significant role in platelet function , almost exclusively in the glycoprotein ( GP ) VI / Fc receptor gamma chain complex mediated signaling pathway . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| TSH and cAMP increased the tyrosine phosphorylation of TSHR and the association between TSHR and the p85alpha regulatory subunit of PI3K . ^^^ The TSH induced S6K1 phosphorylation was inhibited by a dominant negative p85alpha regulatory subunit or by the PI3K inhibitors wortmannin and LY 294002 . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In contrast , overexpression of a dominant negative mutant of the p85alpha regulatory subunit of PI3K ( Deltap 85 ) induced apoptosis . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The alternative PI3K ( phosphoinositide 3 kinase ) inhibitor LY 294002 ( 100 microM ) and a dominant negative form of the enzyme ( p85alpha DeltaiSH 2 ) induce a more modest vesicle enlargement . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that PI3K activation by Reelin requires Src family kinase activity and depends on the Reelin triggered interaction of Dab 1 with the PI3K regulatory subunit p85alpha . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| AR interacts with the p85alpha regulatory subunit of PI3K , and its binding affinity is increased after androgen stimulation . ^^^ Neither N terminal truncated nor proline rich region deleted AR mutants , which are unable to bind to p85alpha and Src , respectively , was able to mediate androgen induced PI3K / Akt activation . ^^^ These findings indicate that a triple complex between AR , p85alpha , and Src is required for androgen stimulated PI3K / Akt activation , and that the PI3K / Akt pathway , in addition to mitogen activated protein kinase , mediates androgen induced cell growth and cell survival . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Contrary to expectations , four different experiments indicated that PI3K is not necessary for JSRV induced transformation : ( 1 ) cotransfection with a dominant negative truncated form of the PI3K regulatory subunit ( Deltap 85 ) did not affect transformation frequency , ( 2 ) cells stably expressing Deltap 85 showed the same frequencies of transformation as parental NIH 3T3 cells , ( 3 ) fibroblasts established from double knockout mice lacking PI3K p85alpha and p85beta could be transformed with JSRV envelope , and ( 4 ) incubation of cells with the PI3K inhibitor LY 294002 did not specifically inhibit transformation , nor did the drug reverse transformation of JSRV transformed cells . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We examined the role of class IA PI3K in antigen induced airway inflammation and hyperresponsiveness by i . p . administration into mice of Deltap 85 protein , a dominant negative form of the class IA PI3K regulatory subunit , p85alpha , which was fused to HIV TAT ( TAT Deltap 85 ) . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Three genes ( recombination activating gene 1 ( RAG 1 ) , heat shock 60 kDa protein 1 ( HSP 60 ) , and transforming growth factor beta 1 ( TGF beta 1 ) ) were found to be up regulated more than two fold in CD , whereas four genes ( phosphoinositide 3 kinase regulatory subunit polypeptide 1 [ p 85 alpha ] ( PI3K ) , frizzled homolog 2 [ Drosophila ] , Bcl 2 / adenovirus E1B 19 kDa interacting protein ( NIP 3 ) , and glia maturation factor beta ( GMF beta ) ) were down regulated to less than 50 % of their normal levels . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Nuclear PLZF binds to a consensus sequence of the phosphatidylinositol 3 kinase p 85 alpha subunit ( p 85 alpha PI3K ) gene . ^^^ AT ( 2 ) enhances expression of p 85 alpha PI3K followed by enhanced p 70 ( S 6 ) kinase , essential to protein synthesis . ^^^ This cardiac selective pathway involving AT ( 2 ) , PLZF and p 85 alpha PI3K may explain the absence of a cardiac hypertrophic response in AT ( 2 ) gene deleted mice . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| PI3K activity was assessed by measuring the phosphorylation of the regulatory subunit p85alpha and kinase activity of the catalytic 110 kDa subunit of PI3K . ^^^ ANG 2 increased the phosphorylation of p85alpha and kinase activity of the 110 kDa PI3K subunit in VSMCs from SHR and transiently increased p85alpha SAM 68 association . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In addition , analysis of mice lacking individual PI3K genes indicates that products of the Pik3r1 gene contribute to transformation efficiency by BCR ABL . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We generated an intracellular single chain B 1 8 Fv ( iscFv ) , fused it to the N terminus of the regulatory subunit ( p85alpha ) of phosphatidylinositol 3 kinase ( PI3K ) ( isc p85alpha ) , and examined the potential of this iscFv to serve as an intracellular elutable protein purification tag . ^^^ Furthermore , co purification of the catalytic subunit of PI3K ( p 110 ) was achieved from lysates of co transfected S 2 cells as well as RBL 2H3 mast cells stably expressing isc p85alpha . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The aim of our study was to investigate whether common polymorphisms in the genes regulating the early insulin signalling pathway ( insulin ; A 23T , insulin like growth factor 1 receptor [ IGF 1R ] ; GAG1013GAA , plasma cell membrane glycoprotein 1 [ PC 1 ] ; K121Q , insulin receptor substrate [ IRS 1 ] ; G972R , insulin receptor substrate 2 [ IRS 2 ] ; G1057D and phosphatidylinositol 3 kinase p 85 alpha [ PI3K ] ; M326I ) affect the weight change and development of Type 2 diabetes in the Finnish Diabetes Prevention Study . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| To further study their unique biochemical properties , the three human Class Ia PI3K ( alpha , beta , and delta ) p 110 catalytic domains were cloned and co expressed with the p85alpha regulatory domain in Sf 9 cells . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We find that , although the two stimuli result in comparable recruitment of the p85alpha subunit of PI3K into complexes with tyrosine phosphorylated proteins , the p85beta regulatory subunit and p110alpha catalytic subunit of PI3K are preferentially recruited into these complexes in response to IGF 1 . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Expression of p85alpha and Akt mutants , or pretreatment of cells with LY 294002 , a PI3K inhibitor , attenuated sPLA ( 2 ) induced MMP 2 activation and migration . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Tumors exhibited increased association of Egfr with clathrin heavy chain ( CHC ) , Gab 1 , and p85alpha , the regulatory subunit of phosphoinositide 3 kinase ( PI3K ) , and tumors also overexpressed c Src , PDK 1 , and Akt . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Ex vivo biochemical studies of mediobasal hypothalamic tissue revealed that insulin stimulated the association of insulin receptor substrate 1 with the p85alpha subunit of PI3K and also tyrosine phosphorylation of p 42 and p 44 subunits of MAPK in the hypothalamus . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In clone no . 6 30 in which the gene coding for the p85alpha subunit of phosphoinositide 3 kinase ( PI3K ) was trapped , the expression of marker genes of early chondrocytes including collagen type 2 , aggrecan , and PTH / PTHrP receptor was delayed . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Gene targeting experiments had shown that B cells deficient in p85alpha , an adaptor protein required for PI3K function , were defective in their ability to proliferate in response to BCR stimulation . ^^^ Unexpectedly , they show that while the BCR induced phosphorylation of the PI3K dependent kinase Akt is reduced in p85alpha deficient cells , the phosphorylation of two downstream targets of Akt FOXO 1 and ribosomal protein S 6 is largely unaffected . ^^^ Furthermore , they show that treatment of wild type B cells with PI3K inhibitors had a more profound effect than disruption of the p85alpha gene . ^^^ Taken together , these results indicate that in the absence of p85alpha , there is still significant residual PI3K activity . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| B cell receptor ( BCR ) driven proliferation is completely blocked either in cells lacking the p85alpha regulatory isoform of PI3K or in wild type cells treated with pharmacological PI3K inhibitors . ^^^ Here we show that B cells lacking p85alpha have signaling impairments that are both quantitatively and qualitatively different from those in cells treated with PI3K inhibitors . ^^^ These partial impairments suggest that there are other routes to PI3K activation in B cells apart from p85alpha associated catalytic subunits . ^^^ Notably , addition of phorbol ester restores BCR mediated proliferation in p85alpha deficient cells but not wild type cells treated with PI3K inhibitors . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the association between thyroid hormone receptor beta 1 ( TRbeta 1 ) and PI3K regulatory subunit p85alpha , and the inhibition of T 3 induced PI3K activation and mTOR phosphorylation by a dominant negative TR ( G345R ) demonstrated the involvement of TR in this T 3 action . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We demonstrated that CM ( Coll ) Listeria / TSB increases the tyrosine phosphorylation level of ErbB 2 and ErbB 3 , members of the epidermal growth factor receptor ( EGFR ) family , and the association between ErbB 3 and the phosphatidylinositol 3 kinase ( PI3K ) regulatory subunit ( p85alpha ) . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Mice lacking p85alpha , the predominant PI3K regulatory isoform , exhibit defects in B cell development and activation that are grossly similar to those found in mice lacking Bruton ' s tyrosine kinase ( Btk ) and other critical signaling molecules . ^^^ These findings establish a role for PI3K p85alpha in differentiation of both follicular and marginal zone B cells , and suggest that these functions are required not solely for the propagation of anti apoptotic signals . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Moreover , Dex induced an association of GR with the regulatory subunit of PI3K , p85alpha , in a ligand dependent manner and promoted serine / threonine kinase Akt phosphorylation that was blocked by coadministration of mifepristone or LY 294002 . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In situ hybridization histochemistry revealed that the expression of PI3K regulatory subunit alpha isoforms ( p85alpha , p55alpha , and p50alpha ) was significantly enhanced in injured motor neurons , whereas other regulatory subunits such as p85beta or p55gamma were not detected . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| One glioblastoma exhibited a 9 bp deletion that encompassed the exon intron junction of exon 12 of PIK3R1 , documenting for the first time a mutation within a PI3K regulatory subunit in human glioblastoma . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Unexpectedly , mice lacking either of the PI3K regulatory subunits p85alpha or p85beta exhibit increased insulin sensitivity . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| This identified an increase and reduction in the expression of p110gamma and p85alpha class Ia phosphoinositide 3 kinase ( PI3K ) subunits in recurrent tumor epithelia . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Soriano , Development 124 : 2691 2700 , 1997 ) , suggesting that PI3K is an essential mediator of PDGFRalpha signaling at this developmental stage . p85alpha / p55alpha+ / + p50alpha+ / + p85beta / mice had similar but less severe defects , indicating that p85alpha and p85beta have a critical and redundant function in development . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Both effects are associated to the formation of beta catenin / p85alpha and inhibition of beta catenin / APC complexes and are independent of GSK 3 and PI3K activities . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The combination of dominant negative src and fyn blocked calcium induced tyrosine phosphorylation of the regulatory subunit of PI3K , p85alpha , and the activity of the catalytic subunit of PI3K . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The role of the PI3K pathway in pancreatic regeneration after partial Px was assessed by effects of a pharmacologic PI3K inhibitor wortmannin or small interfering RNA ( siRNA ) to the p85alpha regulatory subunit . ^^^ To confirm further the critical role of the PI3K / Akt pathway in pancreatic acinar cell proliferation , IGF 1 mediated cell proliferation was determined in cultured acinar cells pretreated with wortmannin or p85alpha siRNA . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here , we examined the effect of deleting various regulatory subunits of PI3K ( p85alpha and p85beta ) on epithelial neoplasia and lymphoid hyperplasia in PTEN+ / mice . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In the present study we show that , like B lymphocytes lacking cyclin D 2 , the p85alpha subunit of phosphatidylinositol 3 kinase ( PI3K ) or other components of the B cell signalosome , p110delta null B cells fail to induce cyclin D 2 and enter early G 1 but not S phase of the cell cycle . ^^^ Furthermore , using both p85alpha null and p110delta null B cells and inhibitors of PI3K , this study demonstrates for the first time , that BCR cross linking induces cyclin D 2 mRNA expression via transcriptional activation of the cyclin D 2 promoter and that this transcriptional activation of cyclin D 2 requires PI3K activity . ^^^ Further characterisation of signalling intermediates downstream of the BCR revealed a perturbation of MAPK signalling pathways in p85alpha null and p110delta null B cells , and our data suggests that cross talk exists between the PI3K and JNK pathways . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| METHODS : Human colon cancer cells KM 20 and KM12C ( both TRAIL resistant ) were transfected with siRNA directed against the PI3K p85alpha regulatory subunit Akt 1 or nontargeting control sequence and then treated with TRAIL ( 100 ng / mL ) or vehicle . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| To further understand the role of class 1 ( A ) PI3K in controlling heart growth and to circumvent potential complications from the overexpression of dominant negative and constitutively active proteins , we generated mice with muscle specific deletion of the p85alpha regulatory subunit and germ line deletion of the p85beta regulatory subunit of class 1 ( A ) PI3K . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We report that , in mouse embryocarcinoma cells ( F 9 cells ) , RA induces an early activation of PI3K and Akt via an increase in the expression of the p85alpha regulatory subunit . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here we show that in thyroid tumors , PV mutant bound significantly more to the PI3K regulatory subunit p85alpha , resulting in a greater increase in the kinase activity than did TRbeta 1 in wild type mice . ^^^ These results suggest that PV , via the activation of p85alpha , could act to affect PI3K downstream signaling in both the nuclear and extranuclear compartments , thereby contributing to thyroid carcinogenesis . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We have found that the p 85 alpha subunit of PI3K binds directly to Rab 5 and possesses GTPase activating protein ( GAP ) activity toward Rab 5 . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In the present study , we demonstrate that the inhibition of PI3K in VSMCs by expression of a dominant negative p85alpha mutant lacking the p 110 binding domain ( Deltap 85 ) , or by treatment of cells with LY 294002 , inhibited Ang 2 stimulated PAI 1 ( plasminogen activator inhibitor 1 ) mRNA expression . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| In the present study , we demonstrate that macrophages deficient for PI3K ( p85alpha regulatory subunit ) are impaired in nitric oxide ( NO ) production upon lipopolysaccharide and interferon gamma stimulation and thus vulnerable for intracellular bacterial infection such as Chlamydophila pneumoniae . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Co immunoprecipitation studies showed a direct interaction of cytosol localized thyroid hormone receptor TRalpha 1 and the p85alpha subunit of PI3K . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| We inhibited PI3K activities by the transient expression following nucleofection of dominant negative mutants of either p85alpha or p110gamma in the human myeloid cell line PLB 985 , which can be induced to express a neutrophil like phenotype . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| The authors determined the pattern of distribution of PI3K pathway components ( ie , the p85alpha regulatory subunit , p110alpha catalytic subunit , Akt 1 , Akt 2 , and the tumor suppressor PTEN ) in human colorectal cancer . ^^^ METHODS : Immunohistochemical analysis was performed on colorectal adenocarcinomas and adjacent normal mucosa for PI3K pathway components , including p85alpha , p110alpha , Akt 1 , Akt 2 , and the tumor suppressor PTEN , which inhibits PI3K . ^^^ RESULTS : PI3K pathway components p85alpha and Akt 2 were highly expressed in glandular elements of colon cancers , with a correlation between staining intensity and clinical stage ; PTEN expression was decreased in the colon cancers of all stages . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Here , we show that mice with a liver specific deletion of the p85alpha regulatory subunit of PI3K ( L Pik3r1KO ) exhibit a paradoxical improvement of hepatic and peripheral insulin sensitivity . ^^^ Thus , the regulatory subunit p85alpha is a critical modulator of insulin sensitivity in vivo not only because of its effects on PI3K activation , but also as a regulator of PTEN activity . . ^^^ |
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| Interacting proteins: O00329 and P27986 |
Pubmed |
SVM Score :0.0 |
| Investigation of three genes encoding catalytic subunits of Pi3k ( PIK3CA , PIK3CD , and PIK3C2B ) revealed amplification of PIK3C2B ( 1q32 ) in 6 tumors ( 6 % ) . ^^^ |
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