| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :1.1849422 |
| Expression of calreticulin domains and PDI as fusion proteins with GAL 4 in the yeast two hybrid system revealed that calreticulin interacted with PDI also under normal cellular conditions . 1.1849422^^^ Direct interaction between calreticulin and PDI is also documented by calreticulin affinity chromatography . 0.60217886^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :1.1529321 |
| Using this technique we demonstrate that PDI interacts with calreticulin at low Ca2+ concentration ( below 100 microM ) , whereas the protein complex dissociates at > 400 microM Ca2+ . 1.1529321^^^ The N domain of calreticulin interacts with PDI , but Ca2+ binding to the C domain of the protein is responsible for Ca2+ sensitivity of the interaction . 0.5011682^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.75723101 |
| An SDK separated as fractions `` TN 31 33 ' ' phosphorylated a 50 kDa substrate which was identified as calreticulin , as well as two endogenous substrates with molecular mass 58 and 55 kDa , both identified as protein disulfide isomerase ( PDI ) . 0.75723101^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| BiP , PDI and the 55 10 10 ( 3 ) Mr protein , respectively , with endoplasmin and the 55 10 10 ( 3 ) Mr protein ( CRP 55 ) accounting for the major proportion of the calcium binding activity . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| The mRNA levels of calreticulin ( CaBP 3 ) increased up to 2 . 3 fold with a kinetic comparable to that of BiP , PDI and hsc 70 , while CaBP 1 and the RER membrane proteins , ribophorin 1 and the signal recognition particle receptor did not show any changes in their relative mRNA amounts after hormonal stimulation . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| We have developed a single purification procedure for the four major resident endoplasmic reticulum ( ER ) proteins : protein disulfide isomerase ( PDI ) , BiP , endoplasmin , and calreticulin . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| We hence redesignate it ERcalcistorin / PDI ( ECaSt / PDI ) , a protein that is distinct from calsequestrin and calreticulin . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Two dimensional immunoblot analysis of the antigenic proteins revealed that the 56kDa and 55kDa proteins showed 4 . 2 and 4 . 0 pI values and were estimated to be protein disulfide isomerase ( PDI ) and calreticulin , respectively , from NH 2 terminal amino acid sequence analysis . ^^^ |
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| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| ERp 60 ( also known as ERp 61 or GRP 58 ) is an isoform of protein disulfide isomerase ( PDI ) that has two thioredoxin like domains a and a ' in positions corresponding to those of domains a and a ' in the PDI polypeptide and shows a significant amino acid sequence similarity to PDI in almost all parts . ^^^ No distinct correlation was found between its exon intron organization and the modular structure of the ERp 60 polypeptide , nor were any similarities in exon intron organization found between the human ERp 60 , PDI , and thioredoxin genes . ^^^ The ERp 60 gene was mapped by fluorescence in situ hybridization to 15q15 and the processed gene to 1q21 , so that neither was located on the same chromosome as the human PDI and thioredoxin genes . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| The pDiTG has no sequence similarity with any of the known TGases , but it has significant homology to protein disulfide isomerase ( PDI ) and , particularly , to the PDI related endoplasmic reticulum protein ERp 60 , a PDI isoform found in the lumen of endoplasmic reticulum . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the three InsP 3 receptor ( InsP3R ) isoforms , two sarco / endoplasmic reticulum Ca2+ ATPase ( SERCA ) isoforms ( 2b and 3 ) as well as four Ca2+ binding proteins ( calreticulin , calnexin , protein disulfide isomerase ( PDI ) and BiP ) , were present in this fraction . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| We report on ECaSt / PDI functioning as a Ca2+ storage protein in the endoplasmic reticulum ( ER ) of a living cell and compare it with calsequestrin and calreticulin , high capacity low affinity Ca2+ binding proteins in the sarcoplasmic reticulum and ER , respectively . ^^^ Microsomes from cells expressing ECaSt / PDI , calreticulin , and calsequestrin accumulated 17 . 2 + / 0 . 27 , 20 . 0 + / 0 . 82 , and 38 . 0 + / 0 . 28 nmol of Ca2+ / mg of protein , respectively ; control microsomes accumulated from 2 . 6 + / 0 . 17 to 2 . 9 + / 0 . 14 nmol of Ca2+ / mg of protein . ^^^ The maximal Ca2+ accumulation by microsomes containing the expressed ECaSt / PDI , C terminally truncated ECaSt / PDI , calreticulin , or calsequestrin correlates approximately with the Ca2+ binding capacity of the respective proteins . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| ERp 60 is a PDI isoform that was initially misidentified as a phosphatidylinositol specific phospholipase C . ^^^ The overall amino acid sequence identity and similarity between the processed human ERp 60 and PDI polypeptides are 29 % and 56 % respectively , and those between the Drosophila ERp 60 and human PDI polypeptides 29 % and 55 % . ^^^ The two ERp 60 polypeptides were found to be similar to human PDI within almost all their domains , the only exception being the extreme C terminal region . ^^^ Additional experiments with hybrid polypeptides in which the C terminal regions had been exchanged between the human ERp 60 and PDI polypeptides demonstrated that the differences in the C terminal region are not the only reason for the lack of prolyl 4 hydroxylase tetramer formation by ERp60 . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| The isoform differs from the PDI / beta and ERp 60 polypeptides in that its N terminal thioredoxin like domain has an unusual catalytic site sequence CVHC . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Although KDEL proteins are primarily localized to the endoplasmic reticulum ( ER ) , we have employed surface biotinylation method to demonstrate that the KDEL proteins calreticulin ( Crt ) , protein disulfide isomerase ( PDI ) and the 78 kDa glucose regulated protein ( GRP 78 ) are found on the surface of the NG 108 15 cell line . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| We show that the endoplasmic reticulum resident oxidoreductases PDI and ERp 57 are directly involved in disulphide oxidation and isomerization , and , together with the lectins calnexin and calreticulin , are central in glycoprotein folding in the endoplasmic reticulum of mammalian cells . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| The overexpression of GFP PDI 57 leads to disruption of endogenous PDI complexes and induces the secretion of PDI , whereas overexpression of a GFP HDEL chimera induces the secretion of endogenous calreticulin , revealing the presence of two independent and saturable mechanisms . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In contrast , the expression of beta 2 microglobulin ( beta 2 m ) , PA28beta , the constitutive proteasome subunits 10 , Y , Z and of the chaperones calnexin , calreticulin , ER 60 and phospho disulfide isomerase ( PDI ) was unaltered or only weakly decreased . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In addition to a TCP 1 related protein , certain members of the heat shock , PDI , and calreticulin family of proteins were detected . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| After in vitro translation and import into microsomes , radiolabeled ERp 57 can be cross linked to endogenous calreticulin and calnexin while radiolabeled PDI can not . ^^^ Likewise , radiolabeled calreticulin is cross linked to endogenous ERp 57 but not PDI . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In the assembly of active prolyl 4 hydroxylase tetramers , the a and b domains of PDI , but not b ' and a ' , can in part be substituted by the corresponding domains of ERp 57 , a PDI isoform that functions naturally in association with the lectins calnexin and calreticulin . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In the presence of proteasomal inhibitors , the ER chaperones calreticulin and calnexin , but not BiP , PDI , or glycoprotein glucosyltransferase , concentrate in the subcellular region of the novel compartment . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Calreticulin , PDI , Grp 94 and BiP chaperone proteins are associated with retained COMP in pseudoachondroplasia chondrocytes . ^^^ Five chaperone proteins : BiP ( Grp 78 ) ; calreticulin ( CRT ) ; protein disulfide ( PDI ) ; ERp 72 ; and Grp 94 , demonstrated immunoreactivity in the enlarged PSACH cisternae and the short rER channels of chondrocytes from both in vivo and in vitro samples . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| These include protein disulphide isomerase ( PDI ) , calreticulin and developing ER specific oleate 12 hydroxylase involved in the biosynthesis of ricinoleic acid . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| D . immitis TGase ( DiTG ) has no sequence similarity with any other known TGase , but has significant similarity to protein disulfide isomerase ( PDI ) related endoplasmic reticulum protein ERp 60 . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The putative surface labeled proteins identified by biotinylation included the molecular chaperones HSP 70 ( MW 70 KDa , pI 5 . 5 ) , HSP90a ( MW 85 KDa , pI 4 . 9 ) , GRP 94 ( MW 92 KDa , pI 4 . 7 ) , GRP 78 ( MW 72 KDa , pI 5 . 0 ) , Oxygen regulated protein 150 ( ORP 150 ; MW 111 KDa , pI 5 . 1 ) , Calreticulin ( MW 48 KDa , pI 4 . 3 ) , Calnexin ( MW 65 KDa , pI 4 . 5 ) , and Protein disulfide isomerase ( PDI ; MW 57 KDa , pI 4 . 8 ) . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In these studies , we have fractionated young and aged liver homogenates , resolved the proteins by 2D gel electrophoresis , assayed for oxidative damage as indicated by protein carbonylation , and identified BiP / Grp78 , protein disulfide isomerase ( PDI ) , and calreticulin as exhibiting an age associated increase in oxidative damage . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| The transcription of key ER proteins including GADD 153 , PERK , GRP 78 , PDI , GRP 94 and calreticulin were up regulated by mefloquine , suggesting that the drug induced an ER stress response . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| The amino acid sequence of ERp 57 , which functions in the endoplasmic reticulum together with the lectins calreticulin and calnexin to achieve folding of newly synthesized glycoproteins , is highly similar to that of protein disulfide isomerase ( PDI ) , but they have their own distinct roles in protein folding . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Using a quantitative pull down assay to investigate the binding of ERp57 / PDI chimeras to calreticulin , we define the b and b ' domains of ERp 57 as the minimal elements that are sufficient for complex formation . ^^^ Using our understanding of substrate binding to the b ' domain of PDI as a paradigm , we show that alterations to specific residues in the b ' domain of ERp 57 dramatically reduce or completely abolish its binding to calreticulin . ^^^ On the basis of these data , we propose a model where the region of ERp 57 equivalent to the primary substrate binding site of archetypal PDI is occupied by calreticulin and suggest that the ER lectins act as adaptor molecules that define the substrate specificity of ERp57 . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In RAP / cells , three well known ER chaperones , calnexin , calreticulin , and protein disulfide isomerase ( PDI ) , associate with LRP and likely compensate for the absence of RAP . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Amino acid sequence analysis of BmTGase revealed significant homology to the protein disulfide isomerase ( PDI ) , particularly , to the PDI related protein ERp 60 , a PDI isoform found in the lumen of endoplasmic reticulum . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| We have extended our earlier observations of ER chaperone induction with the identification of the additional ER ( ERp 29 , ERp 72 , calreticulin , protein disulfide isomerase ( PDI ) ) , cytoplasmic ( heat shock protein ( HSP ) 70 , HSP 90 ) and mitochondrial ( mtHSP 70 ) upregulated chaperones and folding enzymes . ^^^ |
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| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In contrast with PDI and parallel purified co residents ( calreticulin , ERp 60 ) , native ERp 29 lacked classical chaperone , disulphide reductase and isomerase , and calcium binding activities . ^^^ ERp 29 also did not exhibit helper activity toward calreticulin ( chaperone ) or PDI and ERp 60 ( disulphide reductase ) . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| We show that exposure to valproate increases the expression of chaperones that assist in the folding of proteins in the ER including GRP78 / BiP , GRP 94 , PDI and calreticulin as well as the cytosolic chaperone , HSP 70 . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Additionally , following pre incubation of ERp 57 with calreticulin ( CRT ) , decreased interactions were observed between ERp 57 and PDI , and vice versa . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| In addition , altered expression of several proteins , like GRP 78 , GRP 94 , PDI , calreticulin , annexin , cytokeratins , profilin , heat shock proteins , and ORP 150 have been associated with the development of diabetes . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the consistent increment of heat shock protein 70 family members ( hsp 70 ) together with protein disulfide isomerase ( PDI ) at all the examined conditions suggests the possible compensatory function imparted by hsp 70 and PDI family members in the absence of calreticulin and / or calnexin . . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Exposure to atypical antipsychotic drugs such as valproate increases the expression of chaperones that assist in the folding of proteins in the endoplasmic reticulum ( ER ) including calreticulin , GRP78 / BiP , GRP 94 , and PD 1 . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| Proteolytic cleavage accounted for the discrepancies between messenger RNA and protein level changes for 7 genes including calreticulin , PDIA 3 , PDI , and GRP 78 . ^^^ |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13087 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|