Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
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Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.76636092
In addition , calreticulin has sequence similarities with the molecular chaperone calnexin . 0.76636092^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.65575537
Thus , calreticulin appeared to be a lectin with similar oligosaccharide specificity as its membrane bound homologue , calnexin . 0.65575537^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :1.4461966
As a molecular chaperone , calreticulin binds preferentially to nascent glycoproteins via their immature carbohydrates ; this property closely resembles that seen for calnexin , a chaperone with extensive molecular identity to calreticulin . 1.4461966^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.59944127
Furthermore , calreticulin , a chaperone with functional similarities to calnexin , associates with class 1 molecules in beta 2m positive cells . but not in Daudi cells , consistent with completion of folding and disulfide bond formation of class 1 heavy chains before binding to calreticulin occurs . 0.59944127^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The partial cDNA showed sequence homology to the calcium binding region common to calreticulin and calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
CNE 1 , a Saccharomyces cerevisiae homologue of the genes encoding mammalian calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin , calreticulin , and Bip / Kar2p in protein folding . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
We present evidence here that calreticulin , but not calnexin , displays steady state association with class I / TAP complexes . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
We have shown that these glycoproteins interact with BiP , calreticulin and calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Association of ERp 57 with mouse MHC class 1 molecules is tapasin dependent and mimics that of calreticulin and not calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin and calreticulin , both ER resident proteins , have lectin domains that are crucial for their function as chaperones . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Here , EDEM was shown to interact with calnexin , but not with calreticulin , through its transmembrane region . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The endoplasmic reticulum chaperones calnexin and calreticulin were found to be involved in HTNV glycoprotein folding . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
We show that E ( rns ) folding is not assisted by calnexin and calreticulin ; however , the protein strongly interacts with BiP . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Do calnexin and calreticulin have a role in melanin formation . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Moreover , a double knockout mutant of calnexin and calreticulin exhibits additive severe defects . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
As markers of ER mediated phagocytosis , calnexin and calregulin are detectable on non permeabilized fusing macrophages and also concentrated at fusion interfaces where they co localize with actin in permeabilized macrophages / FBGC . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
However , pp 90 revealed a high degree of sequence identity with the Ca ( 2+ ) binding protein , calreticulin . 45Ca2+ overlay studies indicated that pp 90 bound Ca2+ and the name calnexin is proposed . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Here , we focus on calreticulin , a major ER luminal protein , which shares with calnexin two sets of characteristic sequence repeat . ^^^ We constructed a membrane anchored calreticulin chimera by fusing truncated calreticulin to the membrane anchoring tagged segment of calnexin . ^^^ The spectrum of proteins recognized by the membrane anchored calreticulin was remarkably similar to that observed with calnexin . ^^^ We conclude that calreticulin per se is another chaperone with apparently the same characteristics as calnexin and selectively interacts with nascent transferrin in the lumen , suggesting that calreticulin may cover the diversity of maturations . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae CNE 1 encodes an endoplasmic reticulum ( ER ) membrane protein with sequence similarity to calnexin and calreticulin and functions as a constituent of the ER quality control apparatus . ^^^ We have used a polymerase chain reaction strategy to identify in the yeast Saccharomyces cerevisiae genes of the mammalian calnexin / calreticulin family , and we have identified and isolated a single gene , CNE 1 . ^^^ The protein predicted from the CNE 1 DNA sequence shares some of the motifs with calnexin and calreticulin , and it is 24 % identical and 31 % similar at the amino acid level with mammalian calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The fragments , comprising a total of 46 amino acid residues , had an almost 100 % sequence homology with calreticulin and partial homology with calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The deduced amino acid sequence has 58 % homology with dog calnexin ( the ER membrane phosphoprotein of pancreatic cells ) and significant partial homology with calreticulin ( high affinity Ca ( 2+ ) binding protein of the ER membrane ) at the repetitive sequence . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
This sequence is highly conserved when compared to calreticulin ( a luminal ER protein ) , an Onchocerca surface antigen , and yeast and plant calnexin homologues . ^^^ The calnexin amino terminal domain ( residues 1 253 ) also binds Ca2+ , in contrast to the amino terminal domain of calreticulin , which is relatively less acidic . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
A high affinity calcium binding domain , present in both calnexin and calreticulin , and one transmembrane domain similar to that of calnexin were found in the A2 / 6 protein domain . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The postnatal differentiation of sarcoplasmic reticulum ( SR ) of rabbit skeletal muscles ( the slow twitch soleus and the fast twitch adductor muscles ) was monitored between Days 1 and 12 by following on Western blots the expression and accumulation of molecular markers specific not only for the muscle endomembrane system , i . e . , calsequestrin ( CS ) and the ryanodine sensitive Ca2+ release channel , but also for the endoplasmic reticulum ( ER ) at large , i . e . , BiP , calnexin ( CN ) and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The distribution of endoplasmic reticulum ( ER ) markers ( RNA , signal sequence receptor alpha , calnexin , calreticulin , the immunoglobulin binding protein Bip ) and markers of intracellular rapidly exchanging Ca2+ stores [ Ca2+ channels sensitive to either Ins ( 1 , 4 , 5 ) P 3 or ryanodine ) was investigated biochemically and immunologically . ^^^ Bip , calnexin , signal sequence receptor alpha , RNA , did not sediment as either InsP 3 or ryanodine receptors did ; ( c ) calreticulin , an intralumenal low affinity high capacity Ca ( 2+ ) binding protein , had a widespread distribution , similar to that of Bip and calnexin , and was present in Purkinje , granule , Golgi and stellate neurons , as indicated by immunofluorescent labelling of cerebellum cortex cryosections . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In the work described here , purification of Band 7 from dog cardiac SR vesicles , along with amino acid sequencing and cDNA cloning , identified this protein as calnexin , a homologue of calreticulin recently described in dog pancreatic microsomes . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Molecular cloning and expression of SmIrV 1 , a Schistosoma mansoni antigen with similarity to calnexin , calreticulin , and OvRal 1 . ^^^ We report here the molecular cloning and sequencing of SmIrV 1 which contains a deduced amino acid sequence of 582 residues with similarity to three proteins : calnexin , calreticulin , and OvRal 1 , a surface antigen of the filarial nematode Onchocerca volvulus . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Cloning of two cDNAs encoding calnexin like and calreticulin like proteins from maize ( Zea mays ) leaves : identification of potential calcium binding domains . ^^^ Two cDNAs encoding calnexin ( Cln ) like and calreticulin ( Crl ) like proteins have been isolated by immunoscreening of a maize leaf cDNA library . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The glut 1 glucose transporter interacts with calnexin and calreticulin . ^^^ Calreticulin , the soluble homologue of calnexin , was also shown to interact with GT 155 only when the protein bore a correctly modified oligosaccharide group . ^^^ Thus , our data show that both calnexin and calreticulin with Glut 1 in a glycosylation dependent manner . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin and calreticulin promote folding , delay oligomerization and suppress degradation of influenza hemagglutinin in microsomes . ^^^ Calnexin ( CNX ) and calreticulin ( CRT ) are molecular chaperones that bind preferentially to monoglucosylated trimming intermediates of glycoproteins in the endoplasmic reticulum . ^^^ Calnexin ( CNX ) and calreticulin ( CRT ) are molecular chaperones that bind preferentially to monoglucosylated trimming intermediates of glycoproteins in the endoplasmic reticulum . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The related molecular chaperones calnexin and calreticulin differentially associate with nascent T cell antigen receptor proteins within the endoplasmic reticulum . ^^^ In this study , we examined the association of calreticulin , a soluble protein with significant sequence homology to calnexin , with newly synthesized TCR proteins . ^^^ Analogous to calnexin , processing of glycan chains by glucosidase enzymes was required for initial association of TCRalpha and beta proteins with calreticulin ; however , several major differences were noted regarding interaction of calnexin and calreticulin chaperones with TCR proteins . ^^^ First , TCRalpha and beta proteins showed prolonged association with calnexin molecules compared with calreticulin ; interaction of TCRalpha proteins with calreticulin was particularly transient , with most calreticulin TCRalpha protein complexes dissociating within 15 min of their initial assembly . ^^^ Second , we found that , unlike calnexin , which associated with clonotypic TCRalpha and beta proteins and invariant CD3delta and epsilon polypeptides , calreticulin associated specifically with clonotypic TCRalpha and beta proteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calreticulin has sequence homology with the molecular chaperone calnexin , which is known to control folding and assembly of nascent proteins in the endoplasmic reticulum in a calcium dependent manner . ^^^ Our results indicate that calreticulin might have a similar role in protein folding as the chaperone calnexin . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Analysis of protein complexes formed by gB and molecular chaperones in the ER showed that calnexin and calreticulin , lectin like chaperones , bound equal amounts of uncleaved wild type gB , gB deltaI , and gB KNPm , but the glucose regulated proteins 78 ( BiP ) and 94 formed stable complexes only with the mutated forms , causing their retention in the ER . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In contrast , two lumenal markers ( protein disulfide isomerase and calreticulin , the major Ca2+ storage protein of non muscle endoplasmic reticulum ) were enriched in the middle fractions of the velocity gradients while calnexin , a Ca2+ binding membrane protein , was more widely distributed throughout the gradients . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Here , we studied the association of newly synthesized class 1 proteins with calreticulin , a soluble calnexin related ER protein , in murine T cells . ^^^ We found that , unlike calnexin class 1 interactions , calreticulin assembly with class 1 proteins was markedly decreased in the absence of beta 2 microglobulin expression and that calreticulin associated with a subset of class 1 glycoforms distinct from those assembled with calnexin but similar to those bound to TAP ( transporter associated with antigen processing ) proteins . ^^^ The data demonstrate that calnexin and calreticulin chaperones assemble with distinct MHC class 1 assembly intermediates in the ER and show that glycan processing is functionally coupled to release of MHC class 1 proteins from peptide transport molecules . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin and calreticulin interact specifically with newly synthesized glycoproteins in the endoplasmic reticulum ( ER ) and function as molecular chaperones . ^^^ A carbohydrate dependent interaction between newly synthesized glycoproteins , the thiol dependent reductase ERp 57 , and either calnexin or calreticulin was identified . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Class 1 MHC heavy chains associate with many proteins in the endoplasmic reticulum , including TAP , calnexin , calreticulin , and the newly defined tapasin molecule . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
N linked oligosaccharides are necessary and sufficient for association of glycosylated forms of bovine RNase with calnexin and calreticulin . ^^^ Calnexin and calreticulin are lectin like molecular chaperones that promote folding and assembly of newly synthesized glycoproteins in the endoplasmic reticulum . ^^^ To test this notion , glycosylated forms of bovine pancreatic ribonuclease ( RNase ) were translated in the presence of microsomes and their folding and association with calnexin and calreticulin were monitored . ^^^ When expressed with two N linked glycans in the presence of micromolar concentrations of deoxynojirimycin , this small soluble protein was found to bind firmly to both calnexin and calreticulin . ^^^ This indicated that unlike other chaperones , calnexin and calreticulin do not select their substrates on the basis of folding status . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The network of weakly associated resident ER chaperones which included BiP , GRP 94 , calreticulin , calnexin , and other proteins , may serve as a matrix that binds early folding and assembly intermediates and restricts their exit from the ER . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
A subset of these luminal components are specific for glycoproteins , and , like calnexin and calreticulin , the thiol dependent reductase ERp 57 has been shown to interact specifically with soluble secretory proteins bearing N linked carbohydrate . ^^^ Calnexin and calreticulin also interact with glycosylated integral membrane proteins , and in this study we have examined the interaction of ERp 57 with these substrates . ^^^ Furthermore , ERp 57 binds to newly synthesized glycoproteins in combination with either calnexin or calreticulin . ^^^ We propose that ERp 57 acts in concert with calnexin and calreticulin to modulate glycoprotein folding and enforce the glycoprotein specific quality control mechanism operating in the endoplasmic reticulum . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
US 6 protein is found in complexes with TAP1 / 2 , MHC class 1 heavy chain , beta 2 microglobulin , calnexin , calreticulin , and tapasin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In contrast to other known chaperones such as heat shock proteins , BiP and calreticulin , calnexin is an integral membrane protein . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In young and old energy restricted mice , hepatic expression of the endoplasmic reticulum chaperones ERp 57 ( 37 % ) , GRP 170 ( 51 % ) , ERp 72 ( 43 % ) , calreticulin ( 54 % ) and calnexin ( 23 % ) was significantly and specifically reduced . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The CSQ dense vacuoles are shown herein to be specialized ER subdomains as demonstrated by 1 ) the endoglycosidase H sensitivity of their CSQ and 2 ) two markers , calreticulin and calnexin ( but not others , protein disulfide isomerase and BiP ) , intermixed with the vacuole content . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Promotion of transferrin folding by cyclic interactions with calnexin and calreticulin . ^^^ Calnexin , an abundant membrane protein , and its lumenal homolog calreticulin interact with nascent proteins in the endoplasmic reticulum . ^^^ In this system , addition of a glucose donor , UDP glucose , to the microsomes triggered glucosylation of transferrin and resulted in its cyclic interaction with calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The number and location of glycans on influenza hemagglutinin determine folding and association with calnexin and calreticulin . ^^^ Calnexin and calreticulin are homologous molecular chaperones that promote proper folding , oligomeric assembly , and quality control of newly synthesized glycoproteins in the endoplasmic reticulum ( ER ) . ^^^ A large fraction of the HA molecules was found to occur in ternary HA calnexin calreticulin complexes . ^^^ In contrast to calnexin , calreticulin was found to bind primarily to early folding intermediates . ^^^ Analysis of HA mutants with different numbers and locations of N linked glycans showed that although the two chaperones share the same carbohydrate specificity , they display distinct binding properties ; calreticulin binding depends on the oligosaccharides in the more rapidly folding top / hinge domain of HA whereas calnexin is less discriminating . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Quality control in the secretory pathway : the role of calreticulin , calnexin and BiP in the retention of glycoproteins with C terminal truncations . ^^^ They associated with two lectin like endoplasmic reticulum chaperones ( calnexin and calreticulin ) but not BiP / GRP78 . ^^^ Inhibition of the association with calnexin and calreticulin by the addition of castanospermine significantly increased fragment secretion . ^^^ These results indicated that the association with calnexin and calreticulin was involved in retaining the fragments . ^^^ They also suggested that BiP / GRP78 could serve as a backup for calnexin and calreticulin in retaining the fragments . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin and calreticulin bind to enzymically active tissue type plasminogen activator during biosynthesis and are not required for folding to the native conformation . ^^^ The roles of the endoplasmic reticulum lectins calnexin and calreticulin in the folding of tissue type plasminogen activator ( tPA ) have been investigated using an in vitro translation system that reconstitutes these processes as they would occur in the intact cell . ^^^ Using co immunoprecipitation of newly synthesized tPA with antibodies to calnexin and calreticulin , it was demonstrated that the interaction of tPA with both lectins was dependent upon tPA glycosylation and glucosidase trimming . ^^^ When tPA was synthesized in the presence of semi permeabilized cells under conditions preventing complex formation with calnexin and calreticulin , the translation product had a specific plasminogenolytic activity identical with that when synthesized under conditions permitting interactions with both lectins . ^^^ Furthermore , complexes of tPA bound to calnexin and calreticulin were shown to be enzymically active . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In mammalian cells they participate together with calnexin , calreticulin and UDP glucose : glycoprotein glucosyltransferase in the folding and quality control of newly synthesized glycoproteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In higher eukaryotes a quality control system monitoring the folding state of glycoproteins is located in the ER and is composed of the proteins calnexin , calreticulin , glucosidase 2 , and UDP glucose : glycoprotein glucosyltransferase . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Enhanced catalysis of ribonuclease B folding by the interaction of calnexin or calreticulin with ERp 57 . ^^^ The type 1 membrane protein calnexin and its soluble homologue calreticulin are constituents of this system that recognize monoglucosylated N linked glycans that are present on unfolded glycoproteins . ^^^ The endoplasmic reticulum protein ERp 57 is homologous to protein disulfide isomerase and can be cross linked to the same monoglucosylated glycoproteins that bind to calnexin and calreticulin . ^^^ The present study demonstrates that the disulfide isomerase activity of ERp 57 on the refolding of monoglucosylated ribonuclease B is much greater when this glycoprotein is associated with calnexin or calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Furthermore , the three InsP 3 receptor ( InsP3R ) isoforms , two sarco / endoplasmic reticulum Ca2+ ATPase ( SERCA ) isoforms ( 2b and 3 ) as well as four Ca2+ binding proteins ( calreticulin , calnexin , protein disulfide isomerase ( PDI ) and BiP ) , were present in this fraction . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Coordinated participation of calreticulin and calnexin in the biosynthesis of myeloperoxidase . ^^^ Because the membrane bound endoplasmic reticulum protein calnexin is structurally and functionally related to calreticulin , we assessed the role of calnexin in myeloperoxidase biosynthesis . ^^^ Like calreticulin , calnexin coprecipitated exclusively with glycosylated MPO precursors and with apopro MPO but , in contrast to calreticulin , also with the enzymatically active , heme containing precursor pro MPO . ^^^ Transfectants expressing mutant cDNA had prolonged association of MPO related precursors with calreticulin and especially with calnexin . ^^^ These studies demonstrate that 1 ) both calreticulin and calnexin associated with glycosylated apopro MPO ; 2 ) only calnexin associated selectively with the enzymatically active , heme containing precursor pro MPO ; and 3 ) mutants unable to incorporate heme had prolonged association with calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Oligosaccharide binding characteristics of the molecular chaperones calnexin and calreticulin . ^^^ Calnexin and calreticulin are homologous molecular chaperones of the endoplasmic reticulum . ^^^ We compared the oligosaccharide binding specificities of calnexin and calreticulin in an effort to determine the basis for reported differences in their association with various glycoproteins . ^^^ Using mono , di , and oligosaccharides to inhibit the binding of Glc1Man9GlcNAc2 to calreticulin and to a truncated , soluble form of calnexin , we show that the entire Glc alpha 1 3Man alpha 1 2Man alpha 1 2Man structure , extending from the alpha 1 3 branch point of the oligosaccharide core , is recognized by both proteins . ^^^ Consistent with their essentially identical substrate specificities , calnexin and calreticulin exhibited the same relative affinities when competing for binding to the Glc1Man9GlcNAc2 oligosaccharide . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Differential interaction of coagulation factor 8 and factor 5 with protein chaperones calnexin and calreticulin . ^^^ Protein chaperones calnexin ( CNX ) and calreticulin ( CRT ) preferentially interact with glycoproteins containing monoglucosylated N linked oligosaccharides and are proposed to traffic proteins through degradative and / or secretory pathways . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The assembly of newly synthesized MHC class 1 molecules within the endoplasmic reticulum and their association with the transporter associated with antigen processing ( TAP ) is a process involving the chaperones calnexin and calreticulin . ^^^ In addition , ER 60 is part of the late assembly complexes consisting of MHC class 1 , tapasin , TAP , calreticulin and calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Distinct patterns of folding and interactions with calnexin and calreticulin in human class 1 MHC proteins with altered N glycosylation . ^^^ The 176dg mutant with N glycans at positions 86 and 176 , when transfected into CIR cells , demonstrated increased binding to calnexin , detectable both before and after association with beta2m , and reduced interaction with calreticulin and TAP relative to wild type protein bearing a single N glycan at position 86 . ^^^ These data may explain previously noted differences between mouse and human class 1 MHC proteins and demonstrate that the location of N oligosaccharides within proteins can influence their folding and interactions with chaperones such as calnexin and calreticulin . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin , calreticulin , and BiP were shown to interact with E 1 and E 2 , whereas no interaction was detected between GRP 94 and HCV glycoproteins . ^^^ The association of HCV glycoproteins with calnexin and calreticulin was faster than with BiP , and the kinetics of interaction with calnexin and calreticulin were very similar . ^^^ However , calreticulin and BiP interacted preferentially with aggregates whereas calnexin preferentially associated with monomeric forms of HCV glycoproteins or noncovalent complexes . ^^^ Tunicamycin treatment inhibited the binding of HCV glycoproteins to calnexin and calreticulin , indicating the importance of N linked oligosaccharides for these interactions . ^^^ Together , our data suggest that calnexin plays a role in the productive folding of HCV glycoproteins whereas calreticulin and BiP are probably involved in a nonproductive pathway of folding . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Folding of insulin receptor monomers is facilitated by the molecular chaperones calnexin and calreticulin and impaired by rapid dimerization . ^^^ Under normal growth conditions , newly synthesized receptor monomers undergo disulfide bond formation while associated with the homologous chaperones calnexin ( Cnx ) and calreticulin ( Crt ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Coimmunoprecipitation experiments revealed that the cysteine mutations resulted in gB misfolding and retention by the molecular chaperones calnexin , calreticulin , and Grp 78 in the ER . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
A transient complex containing a class 1 heavy chain beta 2 microglobulin ( beta 2 m ) dimer is assembled onto the TAP molecule by successive interactions with the ER chaperones calnexin and calreticulin and a specialized molecule , tapasin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calreticulin and calnexin interact with different protein and glycan determinants during the assembly of MHC class 1 . ^^^ Before peptide binding , a variety of endoplasmic reticulum ( ER ) proteins are associated with class 1 including calnexin , TAP , calreticulin , and tapasin . ^^^ In this study , we present evidence that calreticulin clearly differs from calnexin in how it associates with class 1 . ^^^ Thus , calreticulin is clearly more specific than calnexin in the structures and conformation of the class 1 molecule with which it can interact . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Within the endoplasmic reticulum , factor 8 exhibits stable interaction with protein chaperones identified as the immunoglobulin binding protein ( BiP ) , calnexin and calreticulin . ^^^ Calnexin and calreticulin are transmembrane and lumenal proteins , respectively , localized to the endoplasmic reticulum , which associate transiently with many soluble and membrane glycoproteins during folding and subunit assembly . ^^^ The calnexin and calreticulin interaction with factor 8 occurs primarily through amino terminal linked oligosaccharides within the heavily glycosylated factor 8 B domain and this interaction appears to be required for factor 8 secretion . ^^^ The findings suggest that factor 8 cycles through interactions with BiP , calnexin and calreticulin . ^^^ Although the interaction with BiP does not appear to be required for factor 8 secretion , data suggest that the calnexin and / or calreticulin interaction is required for secretion . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
A gene phylogeny for calreticulin and calnexin sequences in the context of eukaryotic homologues indicates 1 ) that these Ca2+ binding endoplasmic reticulum proteins descend from a gene duplication that occurred in the earliest stages of eukaryotic evolution and furthermore 2 ) that Euglenozoa express the calreticulin protein of the kinetoplastid ( trypanosomes and their relatives ) lineage , rather than that of the eukaryotic chlorophyte which gave rise to Euglena ' s plastids . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Although the precursor synthesized by cells expressing the Y173C mutation ( MPOY173C ) was glycosylated , associated with the molecular chaperones calreticulin and calnexin , and acquired heme , it was neither proteolytically processed to mature MPO subunits nor secreted . ^^^ After prolonged association with calreticulin and calnexin in the endoplasmic reticulum , MPOY173C was degraded . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In humans , after dissociation from calnexin , fully oxidized MHC class 1 heavy chains associate with beta 2 microglobulin ( beta 2m ) and the soluble chaperone calreticulin . ^^^ Recently , the thiol oxidoreductase ERp 57 also known as GRP 58 , ERp 61 , ER 60 , Q 2 , HIP 70 , and CPT and first misidentified as phospholipase C alpha has been shown to bind in conjunction with calnexin or calreticulin to a number of newly synthesized ER glycoproteins when their N linked glycans are trimmed by glucosidases 1 and 2 . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
MHC class 1 molecules assemble in the endoplasmic reticulum ( ER ) with chaperones , including calnexin and calreticulin , before binding to the transporter associated with antigen processing ( TAP ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
However , two other endogenous ER associated proteins , calreticulin and calnexin , occurred in axons as well as in the somatodendritic domains . ^^^ The former appeared also in axons and its localization partially overlapped with that of calreticulin and calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
One function is to assist in the folding of proteins by mediating interactions of the lectin like chaperone proteins calnexin and calreticulin with nascent glycoproteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The influence of oligosaccharides in glycoprotein folding is reviewed as well as the participation of endoplasmic reticulum chaperones ( calnexin and calreticulin ) that recognize monoglucosylated species in the same process . ^^^ The main elements of this control are calnexin and calreticulin as retaining components , the UDP Glc : glycoprotein glucosyltransferase as a sensor of tertiary structures and glucosidase 2 as the releasing agent . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Here , we compared the processing of TCRalpha glycoproteins and their assembly with calnexin and calreticulin chaperones in CD4+CD8+ thymocytes and splenic T cells . ^^^ Interestingly , however , TCRalpha association with the calnexin related molecule calreticulin was decreased in CD4+CD8+ thymocytes compared with splenic T cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
We demonstrate that ERp 57 interacts preferentially with the glycosylated versions of soluble and membrane proteins , and that this interaction occurs in combination with calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Except for monoglucosylated Man 5 species , which are potential substrates for luminal calnexin and calreticulin , the pattern of oligomannosides was similar to that observed on glycoproteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Interaction of monoglucosylated oligosaccharides with ER lectins ( calnexin and / or calreticulin ) facilitates glycoprotein folding but this interaction is not essential for cell viability under normal conditions . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Binding to the lectins calnexin and calreticulin is mediated by monoglucosylated oligosaccharides that are produced transiently by the deglucosylation and reglucosylation of substrate glycoproteins during their maturation process . ^^^ In mammalian cells , calnexin , calreticulin and associated factors promote the correct folding and oligomerization of many glycoproteins , providing unique quality control and chaperone functions specific for glycoproteins in the endoplasmic reticulum . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
It has been proposed that in mammalian cells the monoglucosylated oligosaccharides generated either by partial deglucosylation of the transferred compound or by reglucosylation of glucose free oligosaccharides by the UDP Glc : glycoprotein glucosyltransferase ( GT ) are recognized by ER resident lectins ( calnexin and / or calreticulin ) . ^^^ GII would liberate glycoproteins from the calnexin / calreticulin anchor , but species not properly folded would be reglucosylated by GT , and so continue to be retained by the lectins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Significantly , 6AHA overcame the block in apo ( a ) secretion induced by the ER glucosidase inhibitor , castanospermine . 6AHA may therefore circumvent the requirement for calnexin and calreticulin interaction in apo ( a ) secretion . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
At the same time , molecular chaperones such as glucose regulated protein 94 , calreticulin , calnexin , and protein disulfide isomerase exhibit marked accumulation in these dying cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calreticulin is a lectin like chaperone and , together with calnexin , it plays an important role in quality control during protein synthesis , folding , and posttranslational modification . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Hemagglutinin ' s temporal association with the molecular chaperones calnexin and calreticulin was similar to that observed for their association with elongating ribosome attached nascent chains in live cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In the present study , we HLA genotyped and identified at a biochemical level the three ( HLA A 25 , B 8 , Cw 7 ) class 1 alleles expressed by the previously described [ D ' Urso CM et al ( 1992 ) J Clin Invest 87 : 284 292 ] beta2m defective human melanoma FO 1 cell line and tested their ability to interact with calnexin , calreticulin and the TAP ( transporter associated with antigen processing ) complex . ^^^ All these alleles were found to bind calnexin , but not calreticulin or the poorly expressed TAP complex , both in parental and beta2m transfected FO 1 cells , demonstrating a complex defect of class 1 expression in FO 1 cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Therefore , Myc SERT was co expressed with the endoplasmic reticulum ( ER ) molecular chaperones calnexin , calreticulin and immunoglobulin heavy chain binding protein ( BiP ) , and the foldase , ERp 57 . ^^^ The expression of functional Myc SERT , as determined by an inhibitor binding assay , was enhanced nearly 3 fold by co expressing calnexin , and to a lesser degree on co expression of calreticulin and BiP . ^^^ A physical interaction between Myc SERT calnexin and Myc SERT calreticulin was demonstrated by co immunoprecipitation . ^^^ These associations were inhibited in vivo by deoxynojirimycin , an inhibitor of N glycan precusor trimming that is known to prevent the calnexin / calreticulin N glycan interaction . ^^^ SERT is the first member of the neurotransmitter transporter family whose folding has been shown to be assisted by the molecular chaperones calnexin , calreticulin , and BiP . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Transient association of calnexin and calreticulin with newly synthesized G 1 and G 2 glycoproteins of uukuniemi virus ( family Bunyaviridae ) . ^^^ Here , we show that newly made G 1 and G 2 associate transiently with calnexin and calreticulin , two lectins involved in glycoprotein folding in the ER . ^^^ Stable complexes between G 1 G2 and calnexin or calreticulin could be immunoprecipitated after solubilization of virus infected BHK 21 cells with the detergents digitonin or Triton 10 100 . ^^^ In addition , G 1 G2 calnexin complexes could be recovered after solubilization with CHAPS ( 3 [ ( 3 cholamidopropyl ) dimethylammonio ] 1 propane sulfonate ) , while G 1 G2 calreticulin complexes were not readily detected by using this detergent . ^^^ Sequential immunoprecipitations with anticalreticulin and anticalnexin antisera indicated that about 50 % of newly synthesized G 1 and G 2 was associated with either calnexin or calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Roles of calreticulin and calnexin during mucin synthesis in LS 180 and HT29 / A1 human colonic adenocarcinoma cells . ^^^ We decided to look for possible involvement of the chaperones calreticulin ( CRT ) and calnexin ( CLN ) during synthesis of two similar gastrointestinal mucins , MUC 2 and MUC5AC . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin ( but not calreticulin ) associated with newly synthesized FLAG ( DYKDDDDK epitope ) tagged type 3 IP ( 3 ) R expressed in COS 7 cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
ERp 57 functions as a subunit of specific complexes formed with the ER lectins calreticulin and calnexin . ^^^ In this study we demonstrate that ERp 57 forms discrete complexes with the ER lectins , calnexin and calreticulin . ^^^ After in vitro translation and import into microsomes , radiolabeled ERp 57 can be cross linked to endogenous calreticulin and calnexin while radiolabeled PDI can not . ^^^ Similar results were obtained in Lec 23 cells , which lack the glucosidase 1 necessary to produce glycoprotein substrates capable of binding to calnexin and calreticulin . ^^^ We conclude that ERp 57 forms complexes with both calnexin and calreticulin and propose that it is these complexes that can specifically modulate glycoprotein folding within the ER lumen . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These data show that calnexin interaction with class 1 proteins having truncated N glycans was reduced compared to normal class 1 molecules , whereas the assembly of class 1 with calreticulin and TAP was unperturbed by N glycan chain length . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In the endoplasmic reticulum , MPO precursors interact transiently with calreticulin and calnexin , presumably in their capacity as molecular chaperones . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Analysis of the early biogenesis of CD1b : involvement of the chaperones calnexin and calreticulin , the proteasome and beta ( 2 ) microglobulin . beta ( 2 ) Microglobulin ( beta ( 2 ) m ) associated human CD1b proteins present lipid and glycolipid antigens , which are loaded on CD1b in endosomal compartments . ^^^ In beta ( 2 ) m deficient FO 1 cells , we found CD1b heavy chains ( HC ) complexed with the chaperones calnexin and calreticulin , while MHC class 1 HC associated only with calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Interaction of newly synthesized apolipoprotein B with calnexin and calreticulin requires glucose trimming in the endoplasmic reticulum . ^^^ We have studied the interaction of ApoB with two lectin like chaperones of the Endoplasmic Reticulum ( ER ) Calnexin ( CN ) and Calreticulin ( CR ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
One function of N linked glycans is to assist in the folding of glycoproteins by mediating interactions of the lectin like chaperone proteins calnexin and calreticulin with nascent glycoproteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These include the housekeeping chaperones calnexin and calreticulin and two essential proteins , the transporters associated with antigen processing ( TAP ) for peptide supply , and the protein tapasin which is thought to act as a specialized chaperone . ^^^ We dissected functional effects of processing cofactors by co expressing in insect cells various combinations of the human proteins HLA A 2 , HLA B 27 , beta ( 2 ) microglobulin , TAP , calnexin , calreticulin , and tapasin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Glycosidic processing of asparagine linked Glc ( 3 ) Man ( 9 ) GlcNAc ( 2 ) in the ER leads to the production of monoglucosylated oligosaccharides that promote interaction with the lectin chaperones calreticulin and calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
We show that the endoplasmic reticulum resident oxidoreductases PDI and ERp 57 are directly involved in disulphide oxidation and isomerization , and , together with the lectins calnexin and calreticulin , are central in glycoprotein folding in the endoplasmic reticulum of mammalian cells . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Kinetics of interactions of sendai virus envelope glycoproteins , F and HN , with endoplasmic reticulum resident molecular chaperones , BiP , calnexin , and calreticulin . ^^^ To better understand their maturation processes in the ER , we investigated the time course of their interactions with three ER resident molecular chaperones , BiP , calnexin ( CNX ) , and calreticulin ( CRT ) , in Sendai virus infected HeLa cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The alpha glucosidases participate in glycoprotein folding mediated by calnexin and calreticulin by forming the monoglucosylated high mannose oligosaccharides required for the interaction with the chaperones . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin and calreticulin function via specific carbohydrates in quality control of newly synthesized glycoproteins in the ER , and ERGIC 53 seems to function in the transport of glycoproteins from ER to the Golgi complex . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Many different proteins that are resident in the ER , such as calnexin , transporter associated with antigen processing ( TAP ) , calreticulin , and tapasin , have been found to be integral to class 1 assembly . ^^^ Calreticulin and calnexin contrast in their interactions with class 1 , despite other similarities between these two chaperones . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin and calreticulin binding to human thyroperoxidase is required for its first folding step ( s ) but is not sufficient to promote efficient cell surface expression . ^^^ In the present study it was proposed to determine how calnexin ( CNX ) and calreticulin ( CRT ) contribute to the folding of hTPO . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Newly synthesized glycoproteins interact during folding and quality control in the ER with calnexin and calreticulin , two lectins specific for monoglucosylated oligosaccharides . ^^^ The findings suggest that discrete populations of nonnative conformations are selectively reglucosylated to participate in the calnexin / calreticulin chaperone pathway . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In both types of aggregates , Ig heavy chain binding protein , calnexin , glucose regulated protein 94 , calreticulin , ERp 72 , and protein disulfide isomerase are scarcely found . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Here we investigated why some glycoproteins interact with the molecular chaperone BiP , and others with the calnexin / calreticulin pathway . ^^^ Direct interaction with calnexin and calreticulin without prior interaction with BiP occurred if glycans were present within about 50 residues of the protein ' s NH 2 terminus . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Functional relationship between calreticulin , calnexin , and the endoplasmic reticulum luminal domain of calnexin . ^^^ Calreticulin , a soluble analog of calnexin , is thought to possess similar functions , but these have not been directly demonstrated in vivo . ^^^ Although many glycoproteins bind to both calnexin and calreticulin , there are differences in the spectrum of glycoproteins that each binds . ^^^ Indeed , the functions of calnexin and calreticulin were largely interchangeable . ^^^ However , when calnexin was expressed as a soluble protein in L cells , the pattern of associated glycoproteins changed to resemble that of calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Protein linked monoglucosylated glycans , formed by glucosidase 1 and glucosidase 2 dependent partial deglucosylation of the oligosaccharides transferred from dolichol diphosphate derivatives in N glycosylation ( Glc ( 3 ) Man ( 9 ) GlcNAc ( 2 ) ) , mediate glycoprotein recognition by two ER resident lectins , membrane bound calnexin ( CNX ) and its soluble homologue , calreticulin ( CRT ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The deduced amino acid sequence of the resulting cDNA clones revealed that Ts p 66 belongs to the calreticulin protein family and represents calnexin of T . suis . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Furthermore , since the stability of N glycosylated proteins is known to be regulated by lectin family chaperones , such as calnexin , a type 1 transmembrane protein located in the endoplasmic reticulum ( ER ) , and calreticulin , a soluble protein in the ER lumen , the effect of the processing inhibitors on the interaction of IFN gamma with calnexin and calreticulin was investigated . ^^^ It was found that IFN gamma formed complexes with calnexin and calreticulin in anti CD 3 stimulated lymphocytes . ^^^ Total binding of IFN gamma to calnexin was not affected but that to calreticulin was increased in anti CD 3 stimulated lymphocytes treated with the processing inhibitors . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Furthermore , evidence of protein misfolding was demonstrated by the prolonged association of tyrosinase mutants with calnexin and calreticulin , known ER chaperones that play a key role in the quality control processes of the secretory pathway . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calreticulin ( CRT ) and calnexin ( CLNX ) are lectin chaperones that participate in protein folding in the endoplasmic reticulum ( ER ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin and calreticulin in conjunction with associated chaperones promote correct folding and oligomerization of many glycoproteins in the endoplasmic reticulum ( ER ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These components are calnexin , calreticulin and ERp 57 , and this review focuses on the molecular basis for their capacity to influence glycoprotein folding . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The R273W mutation did not affect the ability of VWF to bind to BiP , Grp 94 , ERp 72 , calnexin , and calreticulin in COS 7 cells . ( Blood . 2000 ; 96 : 560 568 ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Role of calnexin , calreticulin , and endoplasmic reticulum mannosidase 1 in apolipoprotein ( a ) intracellular targeting . ^^^ Using human apo ( a ) transgenic mouse hepatocytes , we analyzed the role of the ER chaperones calnexin ( CNX ) and calreticulin ( CRT ) , and ER mannosidase 1 in apo ( a ) intracellular targeting . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
This processing allows the binding and release of monoglucosylated ( Glc ( 1 ) Man ( 9 ) GlcNAc ( 2 ) ) glycoproteins with calnexin and calreticulin , the lectin like chaperones of the endoplasmic reticulum . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
A heterogeneous expression pattern of MHC class 1 antigens , TAP peptide transporter , proteasome subunits , proteasome activator PA 28 and the chaperones calnexin , calreticulin as well as tapasin was displayed by these tumor cell lines . ^^^ Single or combined deficiencies in the expression and / or function of TAP , LMP 2 , LMP 10 and tapasin were demonstrated in 11 of 12 cell lines studied , whereas the expression of calnexin , calreticulin , beta 2 microglobulin , LMP 7 and PA28alpha was unaltered or only weakly decreased . ^^^ IFN gamma treatment corrected the TAP , LMP and tapasin deficiencies and enhanced the constitutive PA28alpha , LMP 7 , calnexin and calreticulin expression which was accompanied with increased levels of MHC class 1 antigens . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
To test the role of ER luminal environment in apoptosis , we generated HeLa cell lines inducible with respect to calreticulin and calnexin and investigated their sensitivity to drug dependent apoptosis . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Recognition of folding glycoproteins by two resident lectins , membrane bound calnexin and its soluble homolog , calreticulin , is mediated by protein linked monoglucosylated oligosaccharides . ^^^ Further deglucosylation of the oligosaccharides by glucosidase 2 liberates glycoproteins from their calnexin / calreticulin anchors . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Kifunensine inhibition of ER mannosidase 1 also suppressed ERAD in castanospermine treated cells ; thus , suppression of ERAD does not require lectin like binding of ER chaperones calnexin and calreticulin to monoglucosylated oligosaccharides . ^^^ We hypothesize that , in a manner not requiring lectin like activity of calnexin / calreticulin , the recognition or processing of a specific branched N linked mannose structure enhances the efficiency of glycoprotein retrotranslocation from the ER lumen . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In addition , molecular chaperones calreticulin and calnexin interact with normal MPO precursors in a sequential and regulated fashion . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Finally , we show that the constitutive ER FcepsilonRI alpha chain , expressed in the absence of the other FcepsilonRI subunits , associates with the ER lectin like chaperone calnexin , but not the structurally similar ER chaperone calreticulin , presumably through interaction with monoglucosylated alpha chain ER glycoforms . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In contrast , the expression of beta 2 microglobulin ( beta 2 m ) , PA28beta , the constitutive proteasome subunits 10 , Y , Z and of the chaperones calnexin , calreticulin , ER 60 and phospho disulfide isomerase ( PDI ) was unaltered or only weakly decreased . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In the assembly of active prolyl 4 hydroxylase tetramers , the a and b domains of PDI , but not b ' and a ' , can in part be substituted by the corresponding domains of ERp 57 , a PDI isoform that functions naturally in association with the lectins calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
This intermediate complex binds MHC class 1 beta ( 2 ) m dimers , an event accompanied by the loss of calnexin and the acquisition of calreticulin , generating the MHC class 1 loading complex . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In contrast , calnexin , calreticulin , ER 60 , and protein disulfide isomerase expression are unaltered or only marginally suppressed in these cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Rubella virus glycoprotein interaction with the endoplasmic reticulum calreticulin and calnexin . ^^^ In the present study , we established the interaction of the ER chaperone proteins , calreticulin and calnexin , with the RV E 1 and E 2 proteins in cells stably expressing the viral proteins . ^^^ The interaction between E 2 and calnexin was significantly higher than with calreticulin . ^^^ In pulse chase experiments , the half life of the E 2 calnexin was > 45 min , whereas the half life of the calreticulin E 2 interaction was approximately 10 min . ^^^ Further , the drug treatments resulted in a loss of E 1 and E 2 interaction with calreticulin or calnexin , thereby demonstrating that the interaction is through monoglucosylated forms of RV proteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
During the assembly of major histocompatibility complex ( MHC ) class 1 molecules transient associations are formed with the endoplasmic reticulum resident chaperones calnexin and calreticulin , ERp 57 oxidoreductase , and also with tapasin , the latter mediating binding of the class 1 molecules to the transporter associated with antigen processing ( TAP ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Prior to leaving the ER , CD 82 transiently associates with the membrane bound chaperone calnexin but not with its soluble homolog calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
MALDI mass fingerprinting matched 9 out of 25 peptides with calnexin , and amino acid analysis showed homology with this transmembrane calcium binding protein of the calreticulin family . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In the presence of proteasomal inhibitors , the ER chaperones calreticulin and calnexin , but not BiP , PDI , or glycoprotein glucosyltransferase , concentrate in the subcellular region of the novel compartment . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Suppression of stress proteins , GRP 78 , GRP 94 , calreticulin and calnexin in liver endoplasmic reticulum of rat treated with a highly toxic coplanar PCB ] . ^^^ The present study was addressed on the effect of 3 , 3 ' , 4 , 4 ' , 5 pentachlorobiphenyl ( PenCB ) to the expression of molecular chaperon proteins , glucose regulated protein ( GRP ) 78 , GRP 94 , calreticulin and calnexin in liver endoplasmic reticulum of rat by treatment with acute exposure . ^^^ The protein levels of GRP 78 , GRP 94 , calreticulin and calnexin were significantly decreased with the acute exposure . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Recent work provides the first structural insights into the process of glycoprotein folding in the ER involving the lectin chaperones calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Endoplasmic reticulum and cis Golgi localization of human T lymphotropic virus type 1 p 12 ( 1 ) : association with calreticulin and calnexin . ^^^ Moreover , using coimmunoprecipitation assays , we identify the direct binding of p 12 ( 1 ) with both calreticulin and calnexin , resident ER proteins which regulate calcium storage . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In this study we have compared the interaction of human MHC class 1 molecules with IgG heavy chain ( HC ) binding protein ( BiP ) , calnexin , calreticulin , tapasin and TAP in beta ( 2 ) microglobulin ( beta ( 2 ) m ) or TAP deficient cells , as well as in wild type B LCL cells . ^^^ In B LCL cells , HC beta ( 2 ) m associated with calreticulin and tapasin , whereas no interaction with calnexin and BiP was observed . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Glycans mediate the interaction of newly synthesized glycoproteins with some resident ER folding factors , such as calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Unfolded or partially folded E 1 bound to the endoplasmic reticulum chaperones calnexin and ( with lower efficiency ) calreticulin , whereas no binding to BiP / GRP78 or GRP 94 could be detected . ^^^ Release from calnexin and calreticulin was used to assess formation of mature E 1 . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis . ^^^ Calreticulin and calnexin are Ca2+ binding proteins with chaperone activity in the endoplasmic reticulum . ^^^ A dramatic decline in the rate of phagocytosis was observed in double mutants lacking calreticulin and calnexin , whereas only mild changes occurred in single mutants . ^^^ Green fluorescent protein tagged calreticulin and calnexin , expressed in wild type cells , revealed a direct link of the endoplasmic reticulum to the phagocytic cup enclosing a particle , such that the Ca2+ storage capacity of calreticulin and calnexin might directly modulate activities of the actin system during particle uptake . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Both the haemagglutinin ( H ) and fusion ( F ) glycoproteins interacted with calnexin and GRP 78 , whereas interaction with calreticulin was only demonstrated for the H glycoprotein . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Both wild type meprin alpha and the DeltaMAM mutant interact with the molecular chaperones calnexin and calreticulin in the endoplasmic reticulum . ^^^ Thus , calnexin and calreticulin are intimately involved in the correct folding and transport of meprin to the plasma membrane , as well as in retrograde transport of the DeltaMAM mutant to the ubiquitin dependent proteasomal degradative pathway in the cytosol . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Fusion proteins between MPOpro and sTNFR 1 or green fluorescent protein expressed in myeloid 32D , K 562 , or PLB 985 cells did not associate stably with calreticulin or calnexin , molecular chaperones that normally interact transiently with the MPO precursor , but were still efficiently retained in the ER followed by degradation . ^^^ We conclude that normally secreted , nonmyeloid proteins can be targeted efficiently to storage organelles in myeloid cells , that myeloid cells selectively target some proteins for storage but not others , and that MPOpro may contribute to the prolonged ER retention of the MPO precursor independent of the ER molecular chaperones calreticulin and calnexin . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The peptide loading function was specific to calreticulin , since the defect in K 42 could be rectified by transfection with calreticulin but not a soluble form of calnexin , which shares its lectin like activity . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Peptide binding occurs when assembling class 1 molecules associate with a loading complex consisting of the transporter associated with antigen processing ( TAP ) peptide transporter , tapasin , ERp 57 and calreticulin ( CRT ) / calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Localization of the lectin , ERp 57 binding , and polypeptide binding sites of calnexin and calreticulin . ^^^ Calnexin and calreticulin are membrane bound and soluble chaperones , respectively , of the endoplasmic reticulum ( ER ) which interact transiently with a broad spectrum of newly synthesized glycoproteins . ^^^ In addition to sharing substantial sequence identity , both calnexin and calreticulin bind to monoglucosylated oligosaccharides of the form Glc ( 1 ) Man ( 5 9 ) GlcNAc ( 2 ) , interact with the thiol oxidoreductase , ERp 57 , and are capable of acting as chaperones in vitro to suppress the aggregation of non native proteins . ^^^ To understand how these diverse functions are coordinated , we have localized the lectin , ERp 57 binding , and polypeptide binding sites of calnexin and calreticulin . ^^^ Our results indicate that the primary lectin site of calnexin and calreticulin resides within the globular domain , but the results also point to a much weaker secondary site within the arm domain which lacks specificity for monoglucosylated oligosaccharides . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
ERp 57 is a protein chaperone with thiol dependent protein disulfide isomerase and additional activities and recently it has been shown to be involved , in cooperation with calnexin or with calreticulin , in the correct folding of glycoproteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In wild type sciatic nerves , PMP 22 associates in a specific , transient ( t ( 1 / 2 ) approximately equal to 11 min ) , and oligosaccharide processing dependent manner with the lectin chaperone calnexin ( CNX ) , but not calreticulin nor BiP . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin , calreticulin , and ERp 57 cooperate in disulfide bond formation in human CD1d heavy chain . ^^^ Here we show that CD1d associates in the ER with both calnexin and calreticulin and with the thiol oxidoreductase ERp 57 in a manner dependent on glucose trimming of its N linked glycans . ^^^ The formation of at least one of the disulfide bonds in the CD1d heavy chain is coupled to its glucose trimming dependent association with ERp 57 , calnexin , and calreticulin . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Altered ER and mitochondrial Ca ( 2+ ) responses were also observed in cells expressing an N truncated calreticulin but not in cells overexpressing calnexin , a P domain containing chaperone , indicating that the effects were mediated by the unique C domain of calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Association of the thyrotropin receptor with calnexin , calreticulin and BiP . ^^^ In this study , we investigated whether calnexin ( CNX ) , calreticulin ( CRT ) and BiP , three of the main molecular chaperones present in the endoplasmic reticulum , interact with the TSHR and what effects these interactions might have on the folding of the receptor . ^^^ The overexpression of calnexin and calreticulin in these cells stabilizes the receptor during the first hour after its synthesis , whereas the degradation of TSHR increased in a cell line overexpressing BiP and the quantity of TSHR able to acquire complex type oligosaccharides decreased . ^^^ These results show that calnexin , calreticulin and BiP all interact with TSHR and that the choice made between these two chaperone systems is crucial because each of them has distinct effects on the folding and stability of this receptor at the endoplasmic reticulum level . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Folding of thyroglobulin in the calnexin / calreticulin pathway and its alteration by loss of Ca2+ from the endoplasmic reticulum . ^^^ During its initial folding in the endoplasmic reticulum ( ER ) , newly synthesized thyroglobulin ( Tg ) is known to interact with calnexin and other ER molecular chaperones , but its interaction with calreticulin has not been examined previously . ^^^ We find that , in FRTL 5 and PC Cl 3 cells , calnexin and calreticulin interact with newly synthesized Tg in a carbohydrate dependent manner , with largely overlapping kinetics that are concomitant with the maturation of Tg intrachain disulphide bonds , preceding Tg dimerization and exit from the ER . ^^^ Calreticulin co precipitates more newly synthesized Tg than does calnexin ; however , using two different experimental approaches , calnexin and calreticulin were found in ternary complexes with Tg , making this the first endogenous protein reported in ternary complexes with calnexin and calreticulin in the ER of live cells . ^^^ Interestingly , thapsigargin treatment induces the premature exit of Tg from the calnexin / calreticulin cycle , while stabilizing and prolonging interactions of Tg with BiP ( immunoglobulin heavy chain binding protein ) and GRP 94 ( glucose regulated protein 94 ) , two chaperones whose binding is not carbohydrate dependent . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
RESULTS : Calreticulin and calsequestrin ( the two major calcium storage proteins of somatic cells ) , two types of calcium release receptors , the inositol trisphosphate and ryanodine receptors ( InsP ( 3 ) R 2 , RyRs 1 , 2 , 3 ) , and the molecular chaperone , calnexin , were identified in all investigated cell types . ^^^ Calnexin distribution overlapped with that of calreticulin but appeared to be present in distinct subcompartments . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These proteins include the lectin chaperones calnexin ( CNX ) and calreticulin ( CRT ) , the thiol dependent oxidoreductase ERp 57 , the transporter associated with antigen processing ( TAP ) , and the protein tapasin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These steps include cleavage of the signal sequence , glycosylation , binding by the chaperones calnexin and calreticulin , and the oxidoreductase ERp 57 , and oxidation . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calreticulin , a major Ca ( 2+ ) binding ( storage ) chaperone in the endoplasmic reticulum , is a key component of the calreticulin / calnexin cycle which is responsible for the folding of newly synthesized proteins and glycoproteins and for quality control pathways in the endoplasmic reticulum . ^^^ The function of calreticulin , calnexin and other endoplasmic reticulum proteins is affected by continuous fluctuations in the concentration of Ca ( 2+ ) in the endoplasmic reticulum . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
DTT treatment was associated with enhanced binding of apo [ a ] with the endoplasmic reticulum associated chaperone proteins calnexin , calreticulin , and BiP , which was reversible upon DTT removal . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
N linked oligosaccharide processing , but not association with calnexin / calreticulin is highly correlated with endoplasmic reticulum associated degradation of antithrombin Glu 313 deleted mutant . ^^^ In contrast , kifunensine suppressed ERAD even in castanospermine treated cells , suggesting that suppression of ERAD does not always require the binding of lectin like ER chaperones like calnexin and / or calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Synthesis of monoglucosylated high mannose type dodecasaccharide , a putative ligand for molecular chaperone , calnexin , and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
To confirm whether these molecules are accumulating at the oolemmal surface in eggs , we performed immunofluoresence on live , zona free eggs using antibodies to HSP 70 , HSP 90 , GRP 94 , GRP 78 , calreticulin and calnexin . ^^^ RESULTS : The putative surface labeled proteins identified by biotinylation included the molecular chaperones HSP 70 ( MW 70 KDa , pI 5 . 5 ) , HSP90a ( MW 85 KDa , pI 4 . 9 ) , GRP 94 ( MW 92 KDa , pI 4 . 7 ) , GRP 78 ( MW 72 KDa , pI 5 . 0 ) , Oxygen regulated protein 150 ( ORP 150 ; MW 111 KDa , pI 5 . 1 ) , Calreticulin ( MW 48 KDa , pI 4 . 3 ) , Calnexin ( MW 65 KDa , pI 4 . 5 ) , and Protein disulfide isomerase ( PDI ; MW 57 KDa , pI 4 . 8 ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In addition , co immunoprecipitation experiments show association with the ER chaperone calreticulin , but not calnexin , 78 kDa glucose regulated protein ( Grp78 / BiP ) or protein disulfide isomerase . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Using O PFP and O TFA carrying glycosyl halides , trisaccharide ( Manalpha 1 > 2Manalpha1 > 2Man ) and tetrasaccharide ( Glcalpha 1 > 3Manalpha1 > 2Manalpha1 > 2Man ) portions of monoglucosylated high mannose type dodecasaccharide ( Glc ( 1 ) Man ( 9 ) GlcNAc ( 2 ) ) , a putative ligand for the ER chaperon , calnexin and calreticulin , were synthesized . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
TYR remained misfolded as determined by its sensitivity to trypsin digestion and its persistent interaction with the ER resident lectin chaperones calnexin and calreticulin when produced in ER derived microsomes or nonmelanocytic semipermeabilized cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Compromised calnexin function in calreticulin deficient cells . ^^^ Calnexin and calreticulin are molecular chaperones , which are involved in the protein folding , assembly , and retention / retrieval . ^^^ Here we studied protein folding and chaperone function of calnexin in the absence of calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
On co expression of LPL with human molecular chaperones , we found that calreticulin had the most pronounced effects on LPL activity , but calnexin was also effective . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Here we show that there exists such a mechanism , that it is at least in part mediated by the ER chaperones calnexin and calreticulin , and that the retention is important for efficient cis heterodimerization . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
This review discusses the early stages of MHC class 1 maturation regarding BiP and calnexin association , and differences in MHC class 1 heavy chain ( HC ) interaction with calnexin and calreticulin are highlighted . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
A glycoprotein specific system involving the ER lectins calnexin and calreticulin is well documented , but very little is known about mechanisms that may operate for non glycosylated proteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These included the genes coding for the low molecular weight proteins LMP 2 , LMP 7 , LMP 10 , the transporter associated with antigen processing ( TAP 1 , TAP 2 ) , and calnexin , calreticulin , tapasin , PA 28 alpha , PA 28 beta , ERP 59 and ER 60 . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These cells were systematically characterized by Northern and / or Western blotting ( e . g . , mini transcriptome / mini proteome analysis ) for the expression of HLA A , B , C , beta ( 2 ) microglobulin , and the members of the `` antigen processing machinery ' ' of class 1 molecules ( LMP 2 , LMP 7 , TAP 1 , TAP 2 , tapasin , calreticulin , calnexin , and ERp 57 ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
This modification mediates glycoprotein interaction with the folding machinery comprised of calnexin or calreticulin in conjunction with ERp 57 . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
RESULTS : Calnexin , ERp 57 , calreticulin , tapasin , and HLA class 1 antigens were down regulated in 13 , 13 , 24 , 69 , and 78 % of the 70 lesions tested , respectively . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
To overcome this limitation , we have generated human calnexin , ERp 57 , calreticulin and tapasin specific mAb secreting hybridomas from BALB / c mice immunized with peptides and recombinant proteins . ^^^ The mAb TO 5 , TO 2 , TO 11 and TO 3 were shown to be specific for calnexin , ERp 57 , calreticulin and tapasin , respectively , as they react specifically with the corresponding immunizing peptides in ELISA and with the corresponding proteins when tested with human lymphoid cell lysates in Western blotting . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The loading of newly synthesised MHC class 1 molecules ( MHCI ) with peptides requires the involvement of several endoplasmic reticulum ( ER ) resident cofactors including calnexin , calreticulin , transporter associated with antigen processing , ERp 57 and tapasin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Because intracellular inclusions , containing either amyloid beta ( Abeta ) or phosphorylated tau , are the characteristic feature of sporadic inclusion body myositis ( s IBM ) muscle biopsies , we studied expression and immunolocalization of five ER chaperones , calnexin , calreticulin , GRP 94 , BiP / GRP78 , and ERp 72 , in s IBM and control muscle biopsies . ^^^ Our studies provide evidence of the UPR in s IBM muscle and demonstrate for the first time that the ER chaperones calnexin , calreticulin , GRP 94 , BiP / GRP78 , and ERp 72 physically associate with AbetaPP in s IBM muscle , suggesting their playing a role in AbetaPP folding and processing . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin , calreticulin , and ERp 57 : teammates in glycoprotein folding . ^^^ Essential components of this system include the lectin chaperones calnexin ( CNX ) and calreticulin ( CRT ) and their associated co chaperone ERp 57 , a glycoprotein specific thiol disulfide oxidoreductase . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Contrasting functions of calreticulin and calnexin in glycoprotein folding and ER quality control . ^^^ Calreticulin and calnexin are homologous lectins that serve as molecular chaperones for glycoproteins in the endoplasmic reticulum of eukaryotic cells . ^^^ A dramatic loss of stringency in the ER quality control with transport at the cell surface of misfolded glycoprotein conformers is only observed when substrate access to both calreticulin and calnexin is prevented . ^^^ Although not fully interchangeable during assistance of glycoprotein folding , calreticulin and calnexin may work , independently , as efficient and crucial factors for retention in the ER of nonnative polypeptides . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Differential downregulation of endoplasmic reticulum residing chaperones calnexin and calreticulin in human metastatic melanoma . ^^^ Proper assembly of MHC class 1 molecules is dependent on several cofactors , e . g . the chaperones calnexin and calreticulin residing in the endoplasmic reticulum . ^^^ In the present study , we compared melanoma lesions representing different stages of tumor progression with regard to the expression of calnexin and calreticulin in tumor cells by means of immunohistochemistry . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The amino acid sequence of ERp 57 , which functions in the endoplasmic reticulum together with the lectins calreticulin and calnexin to achieve folding of newly synthesized glycoproteins , is highly similar to that of protein disulfide isomerase ( PDI ) , but they have their own distinct roles in protein folding . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Effect of doxycycline regulated calnexin and calreticulin expression on specific thrombopoietin productivity of recombinant Chinese hamster ovary cells . ^^^ In an attempt to increase the specific thrombopoietin ( TPO ) productivity ( q ( TPO ) ) of recombinant Chinese hamster ovary ( rCHO ) cells ( CHO TPO ) , the effect of expression level of calnexin ( CNX ) and calreticulin ( CRT ) on q ( TPO ) was investigated . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The protein interacts with the two lectin chaperones calnexin and calreticulin and thereby promotes the oxidative folding of newly synthesized glycoproteins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Substrate selection by ERp 57 is dependent upon its formation of discrete complexes with two ER resident lectins , soluble calreticulin and membrane bound calnexin . ^^^ Thus , ERp 57 is presented with a preselected set of substrates upon which it can act , and the specific binding of calreticulin and calnexin to ERp 57 is pivotal to the functions of the resulting complexes . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The thiol oxidoreductase endoplasmic reticulum ( ER ) p 57 interacts with newly synthesized glycoproteins through ternary complexes with the chaperones / lectins calnexin or calreticulin . ^^^ On proteasomal inhibition calnexin and calreticulin concentrate in the pericentriolar endoplasmic reticulum derived quality control compartment that we recently described . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The low density lipoprotein receptor related protein associates with calnexin , calreticulin , and protein disulfide isomerase in receptor associated protein deficient fibroblasts . ^^^ In RAP / cells , three well known ER chaperones , calnexin , calreticulin , and protein disulfide isomerase ( PDI ) , associate with LRP and likely compensate for the absence of RAP . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Toward this end , we examined the potential associations of calnexin , calreticulin , Grp 94 , BiP , ERp 57 , and protein disulfide isomerase with each of the three glycoprotein hormone receptors . ^^^ Calnexin , calreticulin , and protein disulfide isomerase were found to associate with the immature forms of all three wild type ( wt ) glycoprotein hormone receptors . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Some of these ( such as glucosidases 1 and 2 , calnexin , and calreticulin ) have a central role in folding and retention , while others ( such as alpha mannosidases and EDEM ) target unsalvageable glycoproteins for ER associated degradation . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
ER 60 domains responsible for interaction with calnexin and calreticulin . ^^^ ER 60 is a thiol oxidoreductase family protein of the endoplasmic reticulum that facilitates the oxidative folding of glycoproteins via interaction with calnexin ( CNX ) and calreticulin ( CRT ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Calnexin , calreticulin and cytoskeleton associated proteins modulate uptake and growth of Legionella pneumophila in Dictyostelium discoideum . ^^^ Phagocytosis assays with specific cellular inhibitors and the effects of well defined host cell mutants revealed that cytoplasmic calcium levels , cytoskeleton associated proteins and the calcium binding proteins of the ER , calreticulin and calnexin , specifically influence the uptake and intracellular growth of L . pneumophila . ^^^ Confocal microscopic time series with green fluorescent protein ( GFP ) tagged calnexin and calreticulin demonstrated the accumulation of both proteins in the phagocytic cup of L . pneumophila infected host cells . ^^^ The cumulative effects of cytosolic calcium levels , the spatial distribution of calnexin and calreticulin , and the defective invasion and replication of L . pneumophila in calnexin and calreticulin minus cells suggest that these factors are part of a regulatory system that leads to the specific vacuole of L . pneumophila . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain , a proposed ligand of lectin chaperone calreticulin and calnexin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The assembly of MHC class 1 molecules with beta ( 2 ) microglobulin and peptides is assisted by the housekeeping chaperones calnexin , calreticulin , and Erp 57 and the dedicated accessory protein , tapasin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
A stable Tn 5B1 4 insect cell line co expressing the recombinant GFPuv beta 1 , 3 N acetylglucosaminyltransferase 2 ( GFPuv beta3GnT2 ) protein fused to a melittin signal sequence with a lectin like molecular chaperone , human calnexin ( hCNX ) or human calreticulin ( hCRT ) , was constructed . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In addition , in 1259MEL cells , the Ag processing machinery components calnexin , calreticulin , and low m . w . polypeptide 10 are down regulated , and HLA A 2 Ags are selectively lost because of a single cytosine deletion in the HLA A 2 gene exon 4 . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Soluble tyrosinase is an endoplasmic reticulum ( ER ) associated degradation substrate retained in the ER by calreticulin and BiP / GRP78 and not calnexin . ^^^ Co immmunoprecipitation experiments showed that soluble tyrosinase interacts with calreticulin and BiP / GRP78 ( and not calnexin ) during its ER transit . ^^^ The lack of specificity for calnexin interaction reveals a novel role for calreticulin in OCAI albinism . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
In vitro and in vivo assays to assess the functions of calnexin and calreticulin in ER protein folding and quality control . ^^^ Calnexin ( CNX ) , an ER membrane protein , and its soluble homologue , calreticulin ( CRT ) , are two important molecular chaperones that contribute to both processes . ^^^ Calnexin ( CNX ) , an ER membrane protein , and its soluble homologue , calreticulin ( CRT ) , are two important molecular chaperones that contribute to both processes . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
By exclusively reglucosylating incompletely folded and assembled glycoproteins , it serves as a folding sensor that prolongs the association of newly synthesized glycoproteins with the chaperone like lectins calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
This cell line synthesized normal levels of the MHC class 1 H chain and beta ( 2 ) microglobulin , as well as normal levels of TAP , tapasin , GRP 78 , calnexin , calreticulin , ERp 57 , and protein disulfide isomerase . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The use of calnexin and calreticulin by cellular and viral glycoproteins . ^^^ Calnexin and calreticulin are homologous lectin chaperones that assist maturation of cellular and viral glycoproteins in the mammalian endoplasmic reticulum . ^^^ Calnexin and calreticulin share the same specificity for monoglucosylated protein bound N glycans but associate with a distinct set of newly synthesized polypeptides . ^^^ We report here that most calnexin substrates do not associate with calreticulin even upon selective calnexin inactivation , while BiP associates more abundantly with nascent polypeptides under these conditions . ^^^ Calreticulin associated more abundantly with orphan calnexin substrates only in infected cells and preferentially with polypeptides of viral origin , showing stronger dependence of model viral glycoproteins on endoplasmic reticulum lectins . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The lectin chaperone calnexin bound to the nascent chain after the addition of two glycans , and calreticulin association followed upon the addition of a third . ^^^ The glycan specific oxidoreductase ERp 57 was cross linked to tyrosinase when calnexin and calreticulin were associated . ^^^ Therefore , tyrosinase maturation initiates cotranslationally with the Hsp 70 system and is handed off to the lectin chaperone system that first uses calnexin before calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
More than one glycan is needed for ER glucosidase 2 to allow entry of glycoproteins into the calnexin / calreticulin cycle . ^^^ Nascent and newly synthesized glycoproteins enter the calnexin ( Cnx ) / calreticulin ( Crt ) cycle when two out of three glucoses in the core N linked glycans have been trimmed sequentially by endoplasmic reticulum ( ER ) glucosidases 1 ( GI ) and 2 ( GII ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Cellular functions of endoplasmic reticulum chaperones calreticulin , calnexin , and ERp 57 . ^^^ Calreticulin , calnexin , and ERp 57 are among the endoplasmic chaperones that interact with partially folded glycoproteins and determine if the proteins are to be released from the endoplasmic reticulum to be expressed , or alternatively , if they are to be sent to the proteosome for degradation . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Ca2+ responses to two chemoattractants , folate and cyclic AMP ( cAMP ) , were assayed in Dictyostelium D . discoideum mutants deficient in one or both of two abundant Ca2+ binding proteins of the endoplasmic reticulum ( ER ) , calreticulin and calnexin . ^^^ Although cytosolic Ca2+ responses in the calnexin and calreticulin mutants are enhanced , the influx of Ca2+ from the extracellular medium into the mutant cells was smaller . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The present review summarizes the results of a legume lectin study as well as those obtained from a similar analysis carried out here on the animal lectins , namely galectins , pentraxins , calnexin , calreticulin and rhesus rotavirus Vp 4 sialic acid binding domain . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The biosynthetic precursor , apoproMPO , is processed slowly in the ER , undergoing cotranslational N glycosylation , transient interactions with the molecular chaperones calreticulin and calnexin , and heme incorporation to generate enzymatically active proMPO that is competent for export into the Golgi . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
The rate of tapasin ERp 57 conjugate formation is unaffected by the absence of beta 2 microglubulin ( beta2m ) , and is independent of calnexin or calreticulin interactions with monoglucosylated N linked glycans . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Immunodepletion and coimmunoprecipitation experiments , performed in this study , detected a preferential association of L 31 reactive , beta2m free H chains with calnexin in beta2m defective cells , and with calreticulin and TAP in beta2m expressing cells . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : Eighty eight surgically removed malignant astrocytic tumors , classified according to the WHO criteria , were stained in immunoperoxidase reactions with monoclonal antibody recognizing monomorphic , locus specific , and allospecific determinants of HLA class 1 antigens , beta 2 microglobulin , APM components ( LMP 2 , LMP 7 , TAP 1 , TAP 2 , calnexin , calreticulin , and tapasin ) , and HLA class 2 antigens . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
We tested for mutant association with transporter associated with antigen processing ( TAP ) , calnexin ( CNX ) , calreticulin ( CLR ) and beta 2 microglobulin ( beta2m ) . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Alternative chaperone machinery may compensate for calreticulin / calnexin deficiency in Caenorhabditis elegans . ^^^ Calreticulin and calnexin are ER resident chaperones well conserved from worm to human . ^^^ Calreticulin / calnexin knock out mice exhibit a severe phenotype , whereas in Caenorhabditis elegans , calreticulin [ crt 1 ( jh 101 ) ] and calnexin [ cnx 1 ( nr 2009 ) ] null mutant worms exhibit only a mild phenotype , suggesting the possible existence of alternative chaperone machinery that can compensate for the deficiency of calreticulin and / or calnexin . ^^^ Interestingly , the consistent increment of heat shock protein 70 family members ( hsp 70 ) together with protein disulfide isomerase ( PDI ) at all the examined conditions suggests the possible compensatory function imparted by hsp 70 and PDI family members in the absence of calreticulin and / or calnexin . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Beyond lectins : the calnexin / calreticulin chaperone system of the endoplasmic reticulum . ^^^ Calnexin and calreticulin are related proteins that comprise an ER chaperone system that ensures the proper folding and quality control of newly synthesized glycoproteins . ^^^ In addition , calnexin and calreticulin possess binding sites for ATP , Ca2+ , non native polypeptides and ERp 57 , an enzyme that catalyzes disulfide bond formation , reduction and isomerization . ^^^ Furthermore , there is clear evidence that ERp 57 participates in glycoprotein biogenesis either alone or in tandem with calnexin and calreticulin . . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
This was supported by the finding that the variant was found to interact with calnexin and calreticulin and accumulated together with these ER chaperones in a specialized juxtanuclear subcompartment of the ER . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
We recently reported the first molecular genetic evidence that Dictyostelium Ca ( 2+ ) responses to chemoattractants include a contribution from the endoplasmic reticulum ( ER ) responses are enhanced in mutants lacking calreticulin or calnexin , two major Ca ( 2+ ) binding proteins in the ER , even though the influx of Ca ( 2+ ) into the mutants is reduced . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
ERp 57 is a thiol oxidoreductase of the endoplasmic reticulum that appears to be recruited to substrates indirectly through its association with the molecular chaperones calnexin and calreticulin . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
VRK2A has a C terminal hydrophobic region that anchors the protein to membranes in the endoplasmic reticulum ( ER ) and mitochondria , and it colocalizes with calreticulin , calnexin and mitotracker ; whereas VRK2B is detected in both the cytoplasm and the nucleus . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Previous studies on the fate of human thyroperoxidase ( hTPO ) molecules have shown that , after being synthesized , these glycoproteins interact with calnexin and calreticulin and that only some of them are able to acquire a partially folded structure . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
Here , we show that not only CNX , but also its homologue calreticulin ( CRT ) , is involved in the biosynthesis of G5 / G8 sterol transporter . ^^^
Interacting proteins: P27797 and P27824	Pubmed	SVM Score :0.0
These include the molecular chaperones calnexin and calreticulin , which enhance the proper folding and subunit assembly of class 1 molecules and also retain assembly intermediates within the ER ; ERp 57 , a thiol oxidoreductase that promotes heavy chain disulfide formation and proper assembly of the peptide loading complex ; tapasin , which recruits class 1 molecules to the TAP peptide transporter and enhances the loading of high affinity peptide ligands ; and Bap 31 , which is involved in clustering assembled class 1 molecules at ER exit sites for export along the secretory pathway . ^^^