| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Roles for calreticulin and a novel glycoprotein , tapasin , in the interaction of MHC class 1 molecules with TAP . ^^^ Further evidence suggests that the interaction of class 1 beta 2m dimers with TAP occurs via a novel uncharacterized 48 kDa glycoprotein , tapasin , which can bind independently to TAP and class 1 beta 2m calreticulin complexes . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Class 1 MHC heavy chains associate with many proteins in the endoplasmic reticulum , including TAP , calnexin , calreticulin , and the newly defined tapasin molecule . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Prominence of beta 2 microglobulin , class 1 heavy chain conformation , and tapasin in the interactions of class 1 heavy chain with calreticulin and the transporter associated with antigen processing . ^^^ To investigate whether other molecules participate in the binding of beta 2m and H chain to TAP and calreticulin , we analyzed the deletion mutant cell line LCL 721 . 220 , which lacks tapasin . ^^^ These results suggest that tapasin is an obligatory mediator of the assemblage of calreticulin / H chain / beta 2m with TAP . . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| US 6 protein is found in complexes with TAP1 / 2 , MHC class 1 heavy chain , beta 2 microglobulin , calnexin , calreticulin , and tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Newly assembled major histocompatibility complex ( MHC ) class 1 molecules , together with the endoplasmic reticulum chaperone calreticulin , interact with the transporter associated with antigen processing ( TAP ) through a molecule called tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Prior to loading antigenic peptides , assembled major histocompatibility complex ( MHC ) class 1 molecules associate with the transporter associated with antigen processing ( TAP ) in a complex which also includes calreticulin and a recently described component , tapasin . ^^^ Furthermore , this interaction with TAP 2 also involves calreticulin and tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| In addition , ER 60 is part of the late assembly complexes consisting of MHC class 1 , tapasin , TAP , calreticulin and calnexin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| A transient complex containing a class 1 heavy chain beta 2 microglobulin ( beta 2 m ) dimer is assembled onto the TAP molecule by successive interactions with the ER chaperones calnexin and calreticulin and a specialized molecule , tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Before peptide binding , a variety of endoplasmic reticulum ( ER ) proteins are associated with class 1 including calnexin , TAP , calreticulin , and tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Most MHC class 1 molecules in the ER that have not yet acquired peptide are simultaneously bound to the transporter associated with antigen processing ( TAP ) , to the 48 kDa glycoprotein tapasin and to the lectin like chaperone calreticulin , in a multicomponent ' loading complex ' . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| This difference in association with peptide loaded class 1 suggests that tapasin functions later than calreticulin during MHC class 1 assembly , and controls peptide loading onto MHC class 1 molecules in the endoplasmic reticulum . . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| This process involves an assembly complex of several proteins , including TAP , tapasin , and calreticulin , all of which associate specifically with the beta 2 microglobulin assembled , open form of the class 1 heavy chain . ^^^ To better comprehend at a molecular level the regulation of class 1 assembly , we have assessed the influence of multiple individual amino acid substitutions in the MHC class 1 alpha 2 domain on interaction with TAP , tapasin , and calreticulin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , the H74L heavy chain was able to enter the defined MHC class 1 pathway and associate with beta2M , calreticulin , tapasin , and TAP . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| These include the housekeeping chaperones calnexin and calreticulin and two essential proteins , the transporters associated with antigen processing ( TAP ) for peptide supply , and the protein tapasin which is thought to act as a specialized chaperone . ^^^ We dissected functional effects of processing cofactors by co expressing in insect cells various combinations of the human proteins HLA A 2 , HLA B 27 , beta ( 2 ) microglobulin , TAP , calnexin , calreticulin , and tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| These pathways may or may not include the interaction of class 1 molecules with the accessory proteins tapasin , calreticulin , ERp 57 , or TAP . 1 would like to suggest that the particular pathway utilized by a given class 1 molecule depends more upon the availability of appropriate peptides rather than on an intrinsic property of the class 1 molecule , and that tapasin may serve a peptide editing function . . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Many different proteins that are resident in the ER , such as calnexin , transporter associated with antigen processing ( TAP ) , calreticulin , and tapasin , have been found to be integral to class 1 assembly . ^^^ TAP , tapasin , and calreticulin bind preferentially to the open form of class 1 , which can be distinguished with the use of a monoclonal antibody specific for this form . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Despite the drastically reduced association with the TAP loading complex , 1 . e . less than 20 % of HLA A 2 expressed in the cells can be co precipitated with either TAP , calreticulin or tapasin , the mutant proteins are expressed on the cell surface in a stable conformation , and bind a complex set of peptides almost identical to that of wild type HLA A 2 . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| These results indicate that in the absence of an interaction with tapasin , Kb molecules fail to assemble with calreticulin and TAP , yet they are still capable of acquiring a diverse array of peptides . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| A heterogeneous expression pattern of MHC class 1 antigens , TAP peptide transporter , proteasome subunits , proteasome activator PA 28 and the chaperones calnexin , calreticulin as well as tapasin was displayed by these tumor cell lines . ^^^ Single or combined deficiencies in the expression and / or function of TAP , LMP 2 , LMP 10 and tapasin were demonstrated in 11 of 12 cell lines studied , whereas the expression of calnexin , calreticulin , beta 2 microglobulin , LMP 7 and PA28alpha was unaltered or only weakly decreased . ^^^ IFN gamma treatment corrected the TAP , LMP and tapasin deficiencies and enhanced the constitutive PA28alpha , LMP 7 , calnexin and calreticulin expression which was accompanied with increased levels of MHC class 1 antigens . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| We found that the mutant had stronger association with TAP , and , in addition , with tapasin and calreticulin . ^^^ These data confirm the importance of position 116 for TAP association , and indicate that ( 1 ) an aspartic acid at the 116 position can facilitate the interaction , and ( 2 ) association with tapasin and calreticulin is affected along with TAP . ^^^ Furthermore , we tested three natural subtypes of HLA B 15 , and found that a B 15 subtype with a tyrosine at position 116 ( B * 1510 ) was strongly associated not only with TAP , but also with tapasin and calreticulin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Before peptide binding these molecules form part of a multisubunit loading complex that also contains the two subunits of the TAP , the transmembrane glycoprotein tapasin , the soluble chaperone calreticulin , and the thiol oxidoreductase ERp 57 . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Once MHC class 1 heavy chain binds beta ( 2 ) microglobulin ( beta ( 2 ) m ) within the endoplasmic reticulum , an assembly complex comprising the class 1 heterodimer , TAP , TAPasin , calreticulin , and possibly Erp 57 is formed before the binding of high affinity peptide . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| During the assembly of major histocompatibility complex ( MHC ) class 1 molecules transient associations are formed with the endoplasmic reticulum resident chaperones calnexin and calreticulin , ERp 57 oxidoreductase , and also with tapasin , the latter mediating binding of the class 1 molecules to the transporter associated with antigen processing ( TAP ) . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Association of ERp 57 with mouse MHC class 1 molecules is tapasin dependent and mimics that of calreticulin and not calnexin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| In this study we have compared the interaction of human MHC class 1 molecules with IgG heavy chain ( HC ) binding protein ( BiP ) , calnexin , calreticulin , tapasin and TAP in beta ( 2 ) microglobulin ( beta ( 2 ) m ) or TAP deficient cells , as well as in wild type B LCL cells . ^^^ In B LCL cells , HC beta ( 2 ) m associated with calreticulin and tapasin , whereas no interaction with calnexin and BiP was observed . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Our results define subtle differences between how B 27 mutants interact with tapasin ( TPN ) and calreticulin ( CRT ) in comparison to similar mutations in other mouse and human class 1 molecules . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| In the class 1 loading complex , calreticulin and the thiol dependent oxidoreductase ER60 / ERp57 appear to support the function of tapasin in an as yet unknown fashion . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Peptide binding occurs when assembling class 1 molecules associate with a loading complex consisting of the transporter associated with antigen processing ( TAP ) peptide transporter , tapasin , ERp 57 and calreticulin ( CRT ) / calnexin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Calreticulin binds to the alpha 1 domain of MHC class 1 independently of tapasin . ^^^ Prior to binding to antigenic peptide , the major histocompatibility complex ( MHC ) heavy chain associates with an assembly complex of proteins that includes calreticulin , tapasin , and the transporter associated with antigen processing ( TAP ) . ^^^ Our data show that calreticulin can bind weakly to Ld without tapasin ' s assistance , and that deglycosylation of the alpha 1 domain results in a primary loss of binding to calreticulin rather than tapasin . ^^^ We have also shown that high amounts of wild type tapasin are still unable to associate with MHC class 1 in the absence of the MHC class I / calreticulin interaction , confirming the central role of calreticulin in the formation of the MHC class 1 assembly complex . . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| The ER resident molecule tapasin ( Tpn ) is uniquely dedicated to tether class 1 molecules jointly with the chaperone calreticulin ( Crt ) and the oxidoreductase ERp 57 to TAP . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Heavy chains were also isolated from Raji cells in multimolecular complexes ( peptide loading complexes ) containing the transporter associated with antigen processing , tapasin and ERp 57 with and without the lectin like folding chaperone , calreticulin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Antigenic peptides are loaded onto class 1 MHC molecules in the endoplasmic reticulum ( ER ) by a complex consisting of the MHC class 1 heavy chain , beta ( 2 ) microglobulin , calreticulin , tapasin , Erp 57 ( ER 60 ) and the transporter associated with antigen processing ( TAP ) . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| These proteins include the lectin chaperones calnexin ( CNX ) and calreticulin ( CRT ) , the thiol dependent oxidoreductase ERp 57 , the transporter associated with antigen processing ( TAP ) , and the protein tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| These included the genes coding for the low molecular weight proteins LMP 2 , LMP 7 , LMP 10 , the transporter associated with antigen processing ( TAP 1 , TAP 2 ) , and calnexin , calreticulin , tapasin , PA 28 alpha , PA 28 beta , ERP 59 and ER 60 . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| These cells were systematically characterized by Northern and / or Western blotting ( e . g . , mini transcriptome / mini proteome analysis ) for the expression of HLA A , B , C , beta ( 2 ) microglobulin , and the members of the `` antigen processing machinery ' ' of class 1 molecules ( LMP 2 , LMP 7 , TAP 1 , TAP 2 , tapasin , calreticulin , calnexin , and ERp 57 ) . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Utilizing LMP 10 as an intracytoplasmic marker and calreticulin and tapasin as ER luminal markers , we show that the modified flow cytometry and cell ELISA are sensitive , simple and reproducible methods to detect HLA class 1 antigen processing machinery components in cells . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| The resulting peptides are thought to be translocated into the lumen of the endoplasmic reticulum ( ER ) by specific transporters associated with antigen presentation ( TAP ) , and loaded onto MHC class 1 molecules by a complex `` loading machinery ' ' ( which includes tapasin , calreticulin and Erp 57 ) . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Calnexin , ERp 57 , calreticulin , tapasin , and HLA class 1 antigens were down regulated in 13 , 13 , 24 , 69 , and 78 % of the 70 lesions tested , respectively . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Here we show that early phagosomes from human dendritic cells ( DCs ) contain the peptide loading complex , incorporating MHC class 1 , beta 2 microglobulin , transporter associated with Ag processing ( TAP ) , calreticulin , tapasin , and ERp 57 . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| To overcome this limitation , we have generated human calnexin , ERp 57 , calreticulin and tapasin specific mAb secreting hybridomas from BALB / c mice immunized with peptides and recombinant proteins . ^^^ The mAb TO 5 , TO 2 , TO 11 and TO 3 were shown to be specific for calnexin , ERp 57 , calreticulin and tapasin , respectively , as they react specifically with the corresponding immunizing peptides in ELISA and with the corresponding proteins when tested with human lymphoid cell lysates in Western blotting . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| The loading of newly synthesised MHC class 1 molecules ( MHCI ) with peptides requires the involvement of several endoplasmic reticulum ( ER ) resident cofactors including calnexin , calreticulin , transporter associated with antigen processing , ERp 57 and tapasin . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| A dynamic multimolecular assembly termed the peptide loading complex ( PLC ) participates in this process and is composed of MHC class 1 molecules , calreticulin , ERp 57 , and tapasin bound to the transporter associated with antigen processing ( TAP ) peptide transporter . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| This hierarchy broke down in cells lacking tapasin but not in cells lacking calreticulin or in cells lacking transporter associated with antigen processing associated ERp 57 . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| For both of these safeguard mechanisms , the MHC class 1 loading complex ( which consists of the peptide transporter TAP , the chaperones tapasin and calreticulin , and the protein disulfide isomerase ERp 57 ) plays a central role . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| The assembly of MHC class 1 molecules with beta ( 2 ) microglobulin and peptides is assisted by the housekeeping chaperones calnexin , calreticulin , and Erp 57 and the dedicated accessory protein , tapasin . ^^^ Tapasin and calreticulin are essential for efficient MHC class 1 assembly , but their precise action during class 1 assembly remains to be elucidated . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| Tapasin is the central stabilizer of this complex , which also includes the transporter associated with antigen processing ( TAP ) , MHC class 1 molecules , the ER chaperone , calreticulin , and the thiol oxidoreductase ERp 57 . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| This cell line synthesized normal levels of the MHC class 1 H chain and beta ( 2 ) microglobulin , as well as normal levels of TAP , tapasin , GRP 78 , calnexin , calreticulin , ERp 57 , and protein disulfide isomerase . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| During the assembly process , a number of chaperones and accessory molecules , such as transporter associated with antigen processing , tapasin , ER 60 , and calreticulin , assist newly synthesized class 1 molecules to facilitate loading of antigenic peptides and to optimize the repertoire of surface class I / peptide complexes . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| The PLC contains a peptide transporter ( transporter associated with antigen processing ) , a thiooxido reductase ( ERp 57 ) , a glycoprotein chaperone ( calreticulin ) , and tapasin , a class 1 specific chaperone . ^^^ We suggest that tapasin is the key chaperone that directs this action of the PLC with secondary contributions from calreticulin and possibly ERp 57 . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| The rate of tapasin ERp 57 conjugate formation is unaffected by the absence of beta 2 microglubulin ( beta2m ) , and is independent of calnexin or calreticulin interactions with monoglucosylated N linked glycans . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| EXPERIMENTAL DESIGN : Eighty eight surgically removed malignant astrocytic tumors , classified according to the WHO criteria , were stained in immunoperoxidase reactions with monoclonal antibody recognizing monomorphic , locus specific , and allospecific determinants of HLA class 1 antigens , beta 2 microglobulin , APM components ( LMP 2 , LMP 7 , TAP 1 , TAP 2 , calnexin , calreticulin , and tapasin ) , and HLA class 2 antigens . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| These include the molecular chaperones calnexin and calreticulin , which enhance the proper folding and subunit assembly of class 1 molecules and also retain assembly intermediates within the ER ; ERp 57 , a thiol oxidoreductase that promotes heavy chain disulfide formation and proper assembly of the peptide loading complex ; tapasin , which recruits class 1 molecules to the TAP peptide transporter and enhances the loading of high affinity peptide ligands ; and Bap 31 , which is involved in clustering assembled class 1 molecules at ER exit sites for export along the secretory pathway . ^^^ |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O15533 and P27797 |
Pubmed |
SVM Score :0.0 |
| NA |
|