| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.60947283 |
| Herein , we identify CAD as a substrate for caspase 3 degradation in both in vitro and in vivo models of apoptosis . 0.60947283^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| When CAD is expressed with ICAD in COS cells or in a cell free system , CAD is produced as a complex with ICAD : treatment with caspase 3 releases the DNase activity which causes DNA fragmentation in nuclei . ^^^ Here we show that caspase 3 cleaves ICAD and inactivates its CAD inhibitory effect . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| The caspase 3 inhibitor Ac DEVD CHO inhibited apoptosis and DNA ladder formation by approximately 50 % , suggesting a approximately 50 % role of caspase 3 activated DNase ( CAD ) . [ Ca2+ ] 1 was increased after 5 Gy . [ Ca2+ ] 1 chelating BAPTA AM ( 5 microM ) and / or DNase gamma inhibiting Zn2+ ( 0 . 5 mM ) inhibited approximately 50 % of induced apoptosis and DNA laddering , indicating a 50 % participation of Ca2+ / Mg2+ dependent DNase gamma . ^^^ CONCLUSIONS : The p 53 Bax mitochondria caspase 3 CAD pathway and the [ Ca+2 ] 1 mediated DNase gamma pathway were involved in the regulation of 10 ray apoptosis in sensitive Molt 4 cells . . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| The complex was treated with caspase 3 , and CAD was purified to homogeneity . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Microinjection of such cells with recombinant AIF only causes peripheral chromatin condensation ( stage 1 ) , whereas microinjection with activated caspase 3 or its downstream target caspase activated DNAse ( CAD ) causes a more pronounced type of chromatin condensation ( stage 2 ) . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Following FAS stimulation , caspase 8 activates caspase 3 , which in turn activates the caspase activated DNAse ( CAD ) by proteolysis of its inhibitor ( ICAD ) . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| The caspase 3 activated DNase ( CAD , CPAN , or DFF 40 ) belongs to the Mg2+ dependent endonucleases . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| The interactions between apoptotic ( caspase 3 and CAD ) and necrotic ( calpain , cathepsin and DNase 2 ) cascades should be studied further . . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Our results support the proposal that CAD participates in caspase 3 mediated internucleosomal DNA fragmentation in the aftermath of SE . . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| The execution phase of apoptosis is characterized by the activation of effector caspases , such as caspase 3 , that cleave key regulatory or structural proteins and in particular activate apoptotic nucleases such as the caspase activated deoxyribonuclease ( CAD ) . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Involvement of conserved histidine , lysine and tyrosine residues in the mechanism of DNA cleavage by the caspase 3 activated DNase CAD . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| No significant difference in the expression of mRNAs of Fas ligand or CAD ( caspase 3 related DNase ) was detected between the involved and uninvolved epidermis . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| DFF40 / CAD is activated by caspase 3 that cuts the nuclease ' s inhibitor DFF45 / ICAD . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| DNA fragmentation is dependent on the DNase CAD , which is released from its inhibitor , ICAD , upon cleavage by caspase 3 . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| In the nucleus , caspase 3 triggers proteolysis of the caspase activated DNA nuclease ( CAD ) inhibitor , causing CAD induction and subsequent DNA degradation . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Expression of caspase activated deoxyribonuclease ( CAD ) and caspase 3 ( CPP 32 ) in the hydropic cochlea of guinea pigs second report . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The treatment with FA+AD induced up regulation of Fas , activation of c Jun N terminal kinase ( JNK ) , which is one of the key molecules of the alternate pathway of Fas induced apoptosis , up regulation of Bax , up regulation and activation of caspase 3 , activation of caspase 3 dependent DNase ( CAD ) , and final DNA fragmentation and apoptotic morphologies in cardiomyocytes . ^^^ CONCLUSIONS : Cardiomyocyte apoptosis by FA+AD depends on the alternate pathway through the JNK , Bax and caspase 3 , and CAD dependent pathways including a positive feedback mechanism of Fas up regulation . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| PML / TNFalpha induced cell death is associated with DNA fragmentation , activation of caspase 3 , 7 , and 8 , and degradation of DNA fragmentation factor / inhibitor of CAD . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Caspase 3 dependent proteolysis of inhibitor caspase activated deoxyribonuclease ( ICAD ) activates caspase activated deoxyribonuclease ( CAD ) , which induces apoptotic internucleosomal DNA degradation . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Expression of caspase activated deoxyribonuclease ( CAD ) and caspase 3 ( CPP 32 ) in the cochlea of cisplatin ( CDDP ) treated guinea pigs . ^^^ The temporal bones were then removed and immunohistochemically stained for CAD and caspase 3 , using the terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labelling method . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| We show here that co expression of murine CAD with either ICAD L or ICAD S in Escherichia coli as well as mammalian cells leads to a functional DFF complex , which after caspase 3 activation releases a nucleolytically active DNase . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| We investigated expression , activation , and / or translocation of micro calpain , lysosome associated membrane protein 1 ( LAMP 1 ) , caspase 3 , and caspase activated DNase ( CAD ) , as well as morphology of the postischemic CA 1 neurons and DNA electrophoresis pattern . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| The temporal bones were then removed and immunohistochemically stained for ssDNA , CAD and caspase 3 . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Recent studies have demonstrated that a caspase activated deoxyribonuclease ( CAD ) causes DNA degradation in nuclei after treatment of cells with caspase 3 . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| DFF40 / CAD primary activated through site specific proteolytic cleavage by caspase 3 . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptotic activation of caspase 3 or 7 results in the cleavage of DFF45 / ICAD and release of active DFF40 / CAD nuclease . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Subcellular fractionation determined levels of CAD were significantly higher in the nuclear fraction of TLE samples compared with controls , and semiquantitative immunohistochemistry revealed cleaved caspase 3 positive cells in TLE sections but not controls . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Erythropoietin activates caspase 3 and downregulates CAD during erythroid differentiation in TF 1 cells a protection mechanism against DNA fragmentation . ^^^ These observations are in contrast to our understanding that DNA is degraded by CAD ( caspase activated DNase ) when ICAD is cleaved by caspase 3 . ^^^ |
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| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| Apoptosis of OSNs was identified using immunohistochemistry for caspase 3 expression , electron microscopy for ultrastructural cellular morphology , and real time polymerase chain reaction for elevated expression of the proapoptotic genes Fas , FasL , p75NGFR , p 53 , Bax , caspase 3 , and CAD . ^^^ |
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| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| We also provided evidence showing that UVA induces caspase 3 and caspase activated DNase ( CAD ) activity in both H2AX wild type and H2AX knockout mouse embryonic fibroblasts ( MEFs ) . ^^^ Taken together , these data indicated that H2AX phosphorylation is required for DNA ladder formation , but not for the activation of caspase 3 ; and the JNK / H2AX pathway cooperates with the caspase 3 / CAD pathway resulting in cellular apoptosis . . ^^^ |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27708 and P42574 |
Pubmed |
SVM Score :0.0 |
| NA |
|