Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.61440073
Here we report that FEN 1 physically interacts with proliferating cell nuclear antigen ( PCNA ) , the processivity factor for DNA polymerases delta and epsilon . 0.61440073^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.79463434
We find that human FEN 1 binds and cleaves such substrates with efficiencies similar to that displayed with naked DNA . 0.79463434^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.56802563
We find that human AP endonuclease 1 ( APE 1 ) physically interacts with flap endonuclease 1 ( FEN 1 ) and with proliferating cell nuclear antigen . 0.56802563^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.64655583
Previously , we isolated and characterized a complementary DNA ( cDNA ) from rice ( Oryza sativa ) encoding a protein which shows homology with the eukaryotic flap endonuclease 1 ( FEN 1 ) . 0.64655583^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The 5 ' 3 ' exonuclease activity of E . coli DNA polymerase 1 and a related enzyme activity in mammalian cell nuclei , DNase 4 , are unable to catalyse the excision of free deoxyribose phosphate from apurinic / apyrimidinic ( AP ) sites incised by an AP endonuclease . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The activities of three human DNA metabolizing enzymes uracil DNA glycosylase , apurinic / apyrimidinic ( AP ) DNA binding protein ( an AP DNA endonuclease ) and the major cellular deoxyribonuclease ( presumably DNase 3 and / or DNase 4 ) were measured in logarithmic growing ( diploid non established ) fibroblast strains , tumor derived cell lines and SV 40 transformed cell lines . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Structural and functional homology between mammalian DNase 4 and the 5 ' nuclease domain of Escherichia coli DNA polymerase 1 . ^^^ The enzyme has been purified from HeLa cells and shown to possess two catalytic properties characteristic of the 5 ' nuclease function of Escherichia coli DNA polymerase 1 , DNase 4 removes single stranded 5 ' regions from splayed arm DNA structures by endonucleolytic incision at the bifurcation point and possesses RNase H activity . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
FEN 1 is implicated in DNA replication and repair in yeast , and the mammalian homolog of yFEN 1 ( DNase 4 , FEN 1 , or MF 1 ) participates in Okazaki fragment maturation . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Second pathway for completion of human DNA base excision repair : reconstitution with purified proteins and requirement for DNase 4 ( FEN 1 ) . ^^^ Efficient repair of gamma ray induced oxidized AP sites in plasmid DNA also required DNase 4 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Characterization of FEN 1 from Xenopus laevis . cDNA cloning and role in DNA metabolism . cDNAs for the Xenopus laevis homologue of the endo / exonuclease FEN 1 ( DNase 4 ) have been cloned using a polymerase chain reaction strategy . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We recently purified and cloned the gene for a DNA structure specific endonuclease , FEN 1 , from murine cells . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
DNA structural elements required for FEN 1 binding . ^^^ In eukaryotic cells , a 5 ' flap DNA endonuclease and a double stranded DNA 5 ' exonuclease activity reside within a 42 kDa enzyme called FEN 1 ( flap endonuclease 1 and 5 ( five ) ' exonuclease ) . ^^^ Like FEN 1 , these related structure specific nucleases recognize and cleave a branched DNA structure called a DNA flap and its derivative , called a pseudo Y structure . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Recently , a structure specific endonuclease , FEN 1 , has been purified and shown to cleave DNA flap structures . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The enzyme described here , flap endonuclease 1 ( FEN 1 ) , cleaves DNA flap strands that terminate with a 5 ' single stranded end . ^^^ In addition to endonuclease activity , FEN 1 has a 5 ' 3 ' exonuclease activity which is specific for double stranded DNA . ^^^ The endo and exonuclease activities of FEN 1 are discussed in the context of DNA replication , recombination and repair . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Calf 5 ' to 3 ' exo / endonuclease , the counterpart of the human FEN 1 and yeast RTH 1 nucleases , performs structure specific cleavage of both RNA and DNA and is implicated in Okazaki fragment processing and DNA repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Human flap endonuclease 1 ( FEN 1 ) is a member of the structure specific endonuclease family and is involved in DNA repair . ^^^ Eight restrictively conserved amino acids in FEN 1 have been converted individually to an alanine to elucidate their roles in specific DNA substrate binding and catalysis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Processing of branched DNA intermediates by a complex of human FEN 1 and PCNA . ^^^ In eukaryotic cells , a 5 ' flap DNA endonuclease activity and a ds DNA 5 ' exonuclease activity exist within a single enzyme called FEN 1 [ flap endo nuclease and 5 ( five ) ' exo nuclease ] . ^^^ FEN 1 is essential for lagging strand DNA synthesis in Okazaki fragment joining . ^^^ Here we find that human PCNA , the processivity factor for eukaryotic polymerases , physically associates with human FEN 1 and stimulates its endonucleolytic activity at branched DNA structures and its exonucleolytic activity at nick and gap structures . ^^^ In contrast , FEN 1 requires a free single stranded 5 ' terminus and appears to load by tracking along the single stranded DNA branch . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Human flap endonuclease 1 ( FEN 1 ) is a structure specific endonuclease and exonuclease which is essential for DNA replication and repair . ^^^ We have cloned a human FEN 1 gene , overexpressed it in Escherichia coli , purified the recombinant protein to near homogeneity , and characterized its cleavage of a flap DNA structure using a novel analytical approach based on flow cytometry . ^^^ With this approach , we were able to measure continuously the kinetics of DNA cleavage by FEN 1 and to separate experimentally the binding and catalysis functions of the enzyme . ^^^ Using the single turnover kinetics as a measure of the amount of enzyme substrate complex present , we estimated the Kd for the FEN 1 flap DNA substrate to be 7 . 5 nM in the absence of Mg2+ and the rate constant for dissociation of the enzyme substrate complex to be 0 . 07 s 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Cip1 / Waf1 disrupts the recruitment of human Fen 1 by proliferating cell nuclear antigen into the DNA replication complex . ^^^ Fen 1 or maturation factor 1 is a 5 ' 3 ' exonuclease essential for the degradation of the RNA primer DNA junctions at the 5 ' ends of immature Okazaki fragments prior to their ligation into a continuous DNA strand . ^^^ Therefore , the polymerase delta PCNA Fen 1 complex has all the activities associated with prokaryotic DNA polymerases involved in replication : 5 ' 3 ' polymerase , 3 ' 5 ' exonuclease , and 5 ' 3 ' exonuclease . ^^^ Although p 21 , a regulatory protein induced by p 53 in response to DNA damage , interacts with PCNA with a comparable Kd ( 10 nM ) and a stoichiometry of three molecules of p 21 per PCNA trimer , a p 21 PCNA Fen 1 complex is not formed . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In addition , the enzyme also cleaved the 5 ' single stranded tails of flap and pseudo Y DNA structures , suggesting that deoxyinosine 3 ' endonuclease is a bacterial functional homologue of human FEN 1 and yeast RTH 1 nucleases . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The FEN 1 family of structure specific nucleases in eukaryotic DNA replication , recombination and repair . ^^^ Among these , FEN 1 is intriguing because it has complex structural preferences ; specifically , it cleaves at branched DNA structures . ^^^ Because of their unique structural specificities , FEN 1 and its family members have important roles in DNA replication , repair and , potentially , recombination . ^^^ Recently , FEN 1 was found to specifically associate with PCNA , explaining some aspects of FEN 1 function during DNA replication and potentially in DNA repair . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Homologous regions of Fen 1 and p21Cip1 compete for binding to the same site on PCNA : a potential mechanism to co ordinate DNA replication and repair . ^^^ We have identified an interaction between PCNA and the structure specific nuclease , Fen 1 , which is involved in DNA replication . ^^^ A PCNA binding peptide from p21Cip1 competes with Fen 1 peptides for binding to PCNA , disrupts the Fen 1 PCNA complex in replicating cell extracts , and concomitantly inhibits DNA synthesis . ^^^ Competition between homologous regions of Fen 1 and p21Cip1 for binding to the same site on PCNA may provide a mechanism to co ordinate the functions of PCNA in DNA replication and repair . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
New enabling technologies have provided the means to measure DNA cleavage by the structure specific nuclease , human Flap Endonuclease ( FEN 1 ) , in the 300 msec time frame . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
When both ligase and FEN 1 / RTH 1 were present simultaneously , some of the 5 ' monoribonucleotides were ligated into DNA , while others were released . ^^^ After FEN 1 / RTH 1 action and extension by polymerization , DNA ligase 1 can join the entirely DNA strands to complete repair . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen ( PCNA ) and shares sequence elements with the PCNA binding regions of FEN 1 and cyclin dependent kinase inhibitor p 21 . ^^^ PCNA binds to flap endonuclease 1 ( FEN 1 ) , a structure specific endonuclease involved in DNA replication . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Like mammalian FEN 1 , the protein has weak 5 ' > 3 ' double stranded DNA specific exonuclease activity . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Proliferating cell nuclear antigen ( PCNA ) has several roles in progression through S phase : it is required for the function of DNA polymerases delta and epsilon and physically associates with the structure specific nuclease FEN 1 that is essential for Okazaki fragment processing . ^^^ The cyclindependent kinase inhibitor p 21 appears to displace FEN 1 from PCNA to inhibit DNA replication and possibly permit participation of PCNA in nucleotide excision repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In this pathway , DNA synthesis was not required for the action of FEN 1 in the presence of PCNA and a replication factor C containing fraction . ^^^ In this pathway , FEN 1 was functional without PCNA and replication factor C but required the DNA synthesis , which led to a flap structure formation . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
By targeting loops on the surface of the PCNA trimer and changing three or four residues at a time to alanine , we found that a region including part of the domain connecting loop of PCNA and loops on one face of the trimer , close to the C termini , is involved in binding to all of the following proteins : DNA polymerase delta , replication factor C , the flap endonuclease Fen 1 , the cyclin dependent kinase inhibitor p 21 and DNA ligase 1 . ^^^ An inhibition of DNA ligation caused by the interaction of PCNA with DNA ligase 1 was found , and we show that DNA ligase 1 and Fen 1 can inhibit DNA synthesis by DNA polymerase delta / PCNA . ^^^ We demonstrate that PCNA must be located below a 5 ' flap on a forked template to stimulate Fen 1 activity , and considering the interacting region on PCNA for Fen 1 , this suggests an orientation for PCNA during DNA replication with the C termini facing forwards , in the direction of DNA synthesis . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
FEN 1 proteins are a family of nucleases essential for lagging strand DNA synthesis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) , a structure specific nuclease , is an essential enzyme for eukaryotic DNA replication and repair . ^^^ Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN 1 , suggesting that the loop is critical for recognition and cleavage of the junction between single and double stranded regions of flap DNA . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In addition , a defect in the 5 ' 3 ' exonuclease domain of DNA polymerase 1 , homologous to the mammalian FEN 1 and the yeast RAD 27 nucleases , leads to a marked increase in repeat expansions characteristic of several genetic disorders . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Mammalian flap endonuclease 1 ( FEN 1 ) is a structure specific metalloenzyme that acts in processing of both the Okazaki fragments during lagging strand DNA synthesis and flap intermediates during DNA damage repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Structure of the DNA repair and replication endonuclease and exonuclease FEN 1 : coupling DNA and PCNA binding to FEN 1 activity . ^^^ Flap endonuclease ( FEN 1 ) removes 5 ' overhanging flaps in DNA repair and processes the 5 ' ends of Okazaki fragments in lagging strand DNA synthesis . ^^^ The crystal structure of Pyrococcus furiosus FEN 1 , active site metal ions , and mutational information indicate interactions for the single and double stranded portions of the flap DNA substrate and identify an unusual DNA binding motif . ^^^ The enzyme ' s active site structure suggests that DNA binding induces FEN 1 to clamp onto the cleavage junction to form the productive complex . ^^^ The conserved FEN 1 C terminus binds proliferating cell nuclear antigen ( PCNA ) and positions FEN 1 to act primarily as an exonuclease in DNA replication , in contrast to its endonuclease activity in DNA repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Processing of an HIV replication intermediate by the human DNA replication enzyme FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
To assess the roles of the active site residues Glu 160 and Asp 181 of human FEN 1 nuclease in binding and catalysis of the flap DNA substrate and in vivo biological processes of DNA damage and repair , five different amino acids were replaced at each site through site directed mutagenesis of the FEN 1 gene . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Detailed biochemical characterisation revealed the presence of DNA ligase 3 , DNA polymerase epsilon , FEN 1 endonuclease , and exonuclease activities of 5 ' 3 ' and 3 ' 5 ' directionality . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Human flap endonuclease 1 ( FEN 1 ) is a member of the structure specific endonuclease family and is essential in DNA replication and repair . ^^^ FEN 1 has specific endonuclease activity for repairing nicked double stranded DNA substrates that have the 5 ' end of the nick expanded into a single stranded tail , and it is involved in processing Okazaki fragments during DNA replication . ^^^ We used small angle 10 ray scattering to obtain global structural information pertinent to nuclease activity from FEN 1 , the D181A mutant , the wild type FEN 1 . 34 mer DNA flap complex , and the D181A . 34 mer DNA flap complex . ^^^ In the absence of magnesium , the FEN 1 . 34 mer DNA flap complex has an Rg value of approximately 34 A , whereas the D181A . 34 mer DNA flap complex self associates , suggesting that a significant protein conformational change occurs by addition of the flap DNA substrate and that Asp 181 is crucial for proper binding of the protein to the DNA substrate . ^^^ A time course change in the scattering profiles arising from magnesium activation of the FEN 1 . 34 mer DNA flap complex is consistent with the protein completely releasing the DNA substrate after cleavage . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endo / exonuclease 1 ( FEN 1 ) recognizes 5 ' flap DNA structures that have been proposed to be important intermediates in DNA replication , repair and recombination , and cleaves the double strand single strand junction of flap substrates . ^^^ Using an in vitro model system , recent studies have shown that FEN 1 is a necessary enzyme for the removal of RNA primers in Okazaki fragment maturation during lagging strand DNA synthesis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
A role for FEN 1 in nonhomologous DNA end joining : the order of strand annealing and nucleolytic processing events . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
A number of other proteins have been implicated in MMR , including DNA polymerase delta , RPA ( replication protein A ) , PCNA ( proliferating cell nuclear antigen ) , RFC ( replication factor C ) , Exonuclease 1 , FEN 1 ( RAD 27 ) and the DNA polymerase delta and epsilon associated exonucleases . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Processing of UV damage in vitro by FEN 1 proteins as part of an alternative DNA excision repair pathway . ^^^ Both nicked and flapped DNA substrates with photolesions ( the latter may be intermediates in DNA polymerase catalyzed strand displacement synthesis ) were cleaved by FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Although the role Fen 1 plays in DNA replication has been well characterized , its function in DNA repair is not so clear . ^^^ The possible roles of Fen 1 in repair have been investigated by examining any changes in level or localization of Fen 1 in response to DNA damaging agents . ^^^ No change has been found in either patterns or levels of Fen 1 expression induced by DNA damaging agents , either in vivo or in vitro . ^^^ This anti Fen 1 antiserum is well suited to the analysis of proliferation in histological material , since ( 1 ) the proportion of labelled cells equals the experimentally determined growth fraction in an experimental xenograft system and ( 2 ) unlike markers such as PCNA , Fen 1 is not induced by DNA damage . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Results show that mismatched bases up to 15 nucleotides from the 5 ' end of an annealed DNA strand change the pattern of FEN 1 cleavage . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The Saccharomyces cerevisiae RAD 27 gene encodes the yeast homologue of the mammalian FEN 1 nuclease , a protein that is thought to be involved in the processing of Okazaki fragments during DNA lagging strand synthesis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Furthermore , several proteins , XPG , FEN 1 , and DNA ligase 1 , recently were shown to competitively bind to the same region of PCNA , the interdomain connector loop , to which DNA polymerase delta or epsilon also binds . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The 5 ' exonuclease domains of the DNA polymerase 1 proteins of Eubacteria and the FEN 1 proteins of Eukarya and Archaea are members of a family of structure specific 5 ' exonucleases with similar function but limited sequence similarity . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The protein fraction PC FII ( phosphocellulose fraction 2 ) , which does not contain RPA and PCNA but otherwise contains all core BER proteins required for PCNA dependent BER ( AP endonuclease , DNA polymerases delta , beta and DNA ligase , and FEN 1 endonuclease ) , had reduced ability to repair plasmid DNA containing AP sites . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The archaeal sequences are highly homologous to those of the eukaryotic Rad 2 family and they cluster with genes of the FEN 1 subfamily , which are known to be involved in DNA replication and repair in eukaryotes . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The flap endonuclease , FEN 1 , plays a critical role in DNA replication and repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The flap endonuclease , FEN 1 , is an evolutionarily conserved component of DNA replication from archaebacteria to humans . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Here we have reconstituted PCNA dependent repair of AP sites with six purified human proteins : AP endonuclease , replication factor C , PCNA , flap endonuclease 1 ( FEN 1 ) , DNA polymerase delta , and DNA ligase 1 . ^^^ Disruption of the PCNA binding site of either FEN 1 or DNA ligase 1 significantly reduced efficiency of AP site repair but did not affect repair patch size . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Inhibition of FEN 1 processing by DNA secondary structure at trinucleotide repeats . ^^^ However , it has been hypothesized that DNA secondary structure may actively participate by preventing FEN 1 cleavage of displaced Okazaki fragments . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
FEN 1 stimulation of DNA polymerase beta mediates an excision step in mammalian long patch base excision repair . ^^^ Here , we demonstrate by immunodepletion experiments that 5 ' dRP N ( 3 ) excision in long patch BER of uracil DNA in a human lymphoid cell extract is , indeed , dependent upon FEN 1 . ^^^ Formation of the excision product 5 ' dRP N ( 3 ) was dependent upon both strand displacement DNA synthesis by beta pol and FEN 1 excision . ^^^ FEN 1 stimulated strand displacement DNA synthesis of beta pol . ^^^ FEN 1 acting either alone , or without DNA synthesis by beta pol , produced a two nucleotide excision product , 5 ' dRP N ( 1 ) , but not 5 ' dRP N ( 3 ) . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The sequence similarity can be extended to other families of nucleases , such as FEN 1 , DNA polymerases , RNaseH and exonuclease 3 , involved in the ion dependent hydrolysis of nucleic acids . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Human flap endonuclease 1 ( FEN 1 ) , an essential DNA replication protein , cleaves substrates with unannealed 5 ' tails . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
It has been shown that flap endonuclease 1 ( FEN 1 ) , a structure specific nuclease , acts on the removal of RNA primers during Okazaki fragment maturation in DNA synthesis . ^^^ These findings suggest that FEN 1 gene expression is inducible during cell proliferation for DNA synthesis , and is down regulated during cell differentiation . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is an important enzyme involved in DNA replication and repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The 10 ray sensitivity of dna 2 mutants is suppressed by overexpression of a 5 ' to 3 ' exonuclease , the yeast FEN 1 structure specific nuclease , encoded by the RAD 27 gene , which also suppresses the growth defect of dna 2 ts mutants . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The C11orf9 gene consists of 26 exons spanning 33 . 1 kb of genomic DNA and is located about 4 . 3 kb centromeric to FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The three genes are clustered within 92 kb of genomic DNA located 2 kb telomeric to FEN 1 and 50 kb centromeric to VMD 2 and are likely to have arisen evolutionarily from gene duplication as they share a remarkably similar exon / intron organization . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
This enhancement was linearly dependent on the amount of APE 1 added , while addition of other BER enzymes , such as DNA ligase 1 and FEN 1 , had no effect . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The temperature dependent lethality of dna 2 ( ts ) mutants was suppressed by overexpression of genes encoding subunits of polymerase delta ( cdc 1 ( + ) and cdc 27 ( + ) ) , DNA ligase 1 ( cdc 17 ( + ) ) , and Fen 1 ( rad 2 ( + ) ) . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Two modes of FEN 1 binding to PCNA regulated by DNA . ^^^ When PCNA was loaded onto a DNA substrate coupled to magnetic beads , it stabilized retention of FEN 1 on the DNA . ^^^ In this DNA dependent binding assay , pcna 79 also stabilized retention of FEN 1 , but pcna 90 was inactive . ^^^ Therefore , in the absence of DNA , FEN 1 interacts with PCNA mainly through the IDCL . ^^^ However , when PCNA encircles the DNA , the C terminal domain of PCNA rather than its IDCL is important for binding FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Mutations in the DNA metabolic genes RAD 27 ( FEN 1 ) , POL 3 ( polymerase delta ) and MMS 19 destabilized widely separated and diverged inverted ALU : s . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The nature of the 5 ' terminus is a major determinant for DNA processing by Schizosaccharomyces pombe Rad2p , a FEN 1 family nuclease . ^^^ The nuclease activity of FEN 1 is essential for both DNA replication and repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We have examined the performance of the FEN 1 enzymes from Archaeoglobus fulgidus and Methanococcus jannaschii and the DNA polymerase 1 homologues from Thermus aquaticus and Thermus thermophilus in the invasive signal amplification reaction . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The Saccharomyces cerevisiae Dna 2 , which contains single stranded DNA specific endonuclease activity , interacts genetically and physically with Fen 1 , a structure specific endonuclease implicated in Okazaki fragment maturation during lagging strand synthesis . ^^^ Based op this information , we propose a new model in which Dna 2 plays a direct role in Okazaki fragment maturation in conjunction with Fen 1 . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Sensitivity of a S . cerevisiae RAD 27 deletion mutant to DNA damaging agents and in vivo complementation by the human FEN 1 gene . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( Fen 1 ) is a structure specific metallonuclease with important functions in DNA replication and DNA repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The products of proliferating cell nuclear antigen ( PCNA ) and flap endonuclease ( FEN 1 ) genes are multifunctional proteins that are involved in DNA replication and damage repair . ^^^ Our results suggest that the coding regions of the PCNA and FEN 1 genes are highly conserved when compared with other DNA repair genes . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is an enzyme that is very important for DNA replication in all eukaryotes because it cleaves the 5 ' DNA flaps that arise between Okazaki fragments . ^^^ In addition , FEN 1 is important for base excision repair and for nonhomologous DNA end joining in all eukaryotes from yeast to human . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Data from Saccharomyces cerevisiae suggest that the flap endonuclease FEN 1 plays a role in expansion of repetitive DNA tracts . ^^^ It has been hypothesized that insufficiency of FEN 1 or a mutant FEN 1 might contribute to the occurrence of expansion events of long repetitive DNA tracts after polymerase slippage events during lagging strand synthesis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
To further understand the role of PARP 1 in BER , we examined the DNA synthesis and flap excision steps in long patch BER using a reconstituted system containing a 34 base pair BER substrate and five purified human enzymes : uracil DNA glycosylase , apurinic / apyrimidinic endonuclease , DNA polymerase beta , flap endonuclease 1 ( FEN 1 ) , and PARP 1 . ^^^ PARP 1 stimulates strand displacement DNA synthesis by DNA polymerase beta in this system ; this stimulation is dependent on the presence of FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Extensive work on the maturation of lagging strands during the replication of simian virus 40 DNA suggests that the initiator RNA primers of Okazaki fragments are removed by the combined action of two nucleases , RNase HI and Fen 1 , before the Okazaki fragments join . ^^^ Here we show that the endonucleases Dna 2 and Fen 1 act sequentially to facilitate the complete removal of the primer RNA . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
RNA primer removal during DNA replication is dependent on ribonucleotide and structure specific RNase H and FEN 1 nuclease activities . ^^^ Data presented here show that RNase HII from A . fulgidus ( aRNase HII ) specifically recognizes RNA DNA junctions and generates products suited for the FEN 1 nuclease , indicating its role in DNA replication . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Recently , we have reconstituted the PCNA dependent AP site repair reaction with six purified human proteins : AP endonuclease , replication factor C ( RFC ) , PCNA , flap endonuclease 1 ( FEN 1 ) , DNA polymerase delta ( pol delta ) , and DNA ligase 1 . ^^^ PCNA can directly interact with RFC , pol delta , FEN 1 and DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Eukaryotic flap endonuclease ( FEN 1 ) is 42 kD single subunit structure specific nuclease that cleaves 5 ' flap strands of the branched DNA structure and possesses 5 ' exonuclease activity . ^^^ FEN 1 participates in DNA replication , repair , and recombination . ^^^ The interaction of FEN 1 with DNA structures generated during replication and repair was studied using two types of photoreactive oligonucleotides . ^^^ Interaction of FEN 1 with both 5 ' and 3 ' ends of the nick or with primer template systems containing 5 ' or 3 ' protruding DNA strands was shown . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We report a novel interaction of the WRN gene product with the human 5 ' flap endonuclease / 5 ' 3 ' exonuclease ( FEN 1 ) , a DNA structure specific nuclease implicated in DNA replication , recombination and repair . ^^^ WS protein ( WRN ) dramatically stimulates the rate of FEN 1 cleavage of a 5 ' flap DNA substrate . ^^^ The WRN FEN 1 functional interaction is independent of WRN catalytic function and mediated by a 144 amino acid domain of WRN that shares homology with RecQ DNA helicases . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
APE 1 initially cleaves the DNA and then facilitates the sequential binding and catalysis by DNA polymerase beta , DNA polymerase delta , FEN 1 , and DNA ligase 1 . ^^^ We show here that p 21 also inhibits PCNA stimulation of long patch base excision repair ( BER ) in vitro . p 21 disrupts PCNA directed stimulation of flap endonuclease 1 ( FEN 1 ) , DNA ligase 1 , and DNA polymerase delta . ^^^ APE 1 initially cleaves the DNA and then facilitates the sequential binding and catalysis by DNA polymerase beta , DNA polymerase delta , FEN 1 , and DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
UV rapidly induces p 33 ( ING1b ) to bind PCNA competitively through this domain , a motif also found in DNA ligase , the DNA repair associated FEN 1 and XPG exo / endonucleases , and DNA methyltransferase . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a 5 ' 3 ' flap exo / endonuclease that plays an important role in Okazaki fragment maturation , nonhomologous end joining of double stranded DNA breaks , and long patch base excision repair . ^^^ A comet assay demonstrated that DNA repair after MMS or UV treatment was impaired in the cells expressing nuclease deficient FEN 1 but not in the cells with double mutated FEN 1 . ^^^ Taken together , these findings suggest that FEN 1 plays an essential role in the DNA repair processes in mammalian cells and that this activity of FEN 1 is PCNA dependent . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Pol delta has been implicated in the Okazaki fragment maturation process for the extension of the newly synthesized fragment and for the displacement of the RNA / DNA segment of the preexisting downstream fragment generating an intermediate flap structure that is the target for the Dna 2 and flap endonuclease 1 ( Fen 1 ) endonucleases . ^^^ Proliferating cell nuclear antigen ( PCNA ) reduced the template dissociation rate of pol delta , thus increasing the processivity of both synthesis and strand displacement , whereas replication protein A ( RP A ) limited the size of the displaced fragment down to 20 30 nucleotides , by generating a `` locked ' ' flap DNA structure , which was a substrate for processing of the displaced fragment by Fen 1 into a ligatable product . ^^^ Our data support a model for Okazaki fragment processing where the strand displacement activity of DNA polymerase delta is modulated by the concerted action of PCNA , RP A and Fen 1 . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The 5 ' > 3 ' exonuclease domain of DNA polymerase 1 is a homolog of the mammalian FEN 1 and the yeast RAD 27 flap nucleases . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The preferred substrate for archaeal FEN 1 or the 5 ' nuclease domains of bacterial DNA polymerases is a double flap structure containing a 3 ' tail on the upstream primer adjacent to the 5 ' flap . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
MMR independent pathways or factors that can process some types of mismatches in DNA are nucleotide excision repair ( NER ) , some base excision repair ( BER ) glycosylases , and the flap endonuclease FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
DNA ligase 1 competes with FEN 1 to expand repetitive DNA sequences in vitro . ^^^ Flap endonuclease 1 ( FEN 1 ) cleaved the displaced downstream strand and together with DNA ligase 1 produced non expanded products . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a critical enzyme for DNA replication and repair . ^^^ An understanding of the ability of FEN 1 to recognize and bind a flap DNA substrate is critical for the elucidation of its molecular mechanism and for the explanation of possible pathological consequences resulting from its failure to bind DNA . ^^^ Furthermore the possible interaction sites of these positively charged residues with DNA substrates were discussed based on FEN 1 cleavage patterns using different substrates . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The cellular proteins flap endonuclease 1 ( FEN 1 ) , proliferating cell nuclear antigen , replication factor C , DNA ligase 1 and DNA polymerase delta are required for the repair of this type of DNA lesion . ^^^ The role of FEN 1 in the base excision repair pathway is to cleave 5 ' unpaired flaps in forked structures so that DNA ligase can seal the single stranded breaks that remain following gap repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease ( Fen 1 ) is required for DNA replication and repair , and defects in the gene encoding Fen 1 cause increased accumulation of mutations and genome rearrangements . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The structure specific FEN 1 endonuclease has been implicated in various cellular processes , including DNA replication , repair and recombination . ^^^ Surprisingly , homozygous mutant ( FEN 1 / ) cells were viable , indicating that FEN 1 is not essential for cell proliferation and thus for Okazaki fragment processing during DNA replication . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Removal of flap DNA intermediates in DNA replication and repair by flap endonuclease 1 ( FEN 1 ) is essential for mammalian genome integrity . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Plumbagin treatment caused a S G ( 2 ) / M phase arrest and cell death of both MEF cell lines , induced p 21 levels , and decreased p 21 mediated long patch ( LP ) BER by blocking DNA ligase activity in the polbeta dependent pathway and by blocking both FEN 1 and DNA ligase activity in polbeta independent pathway . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Specifically , APE 1 enhanced the enzymatic activity of both flap endonuclease 1 ( FEN 1 ) and DNA ligase 1 . ^^^ However once the THF residue was displaced at least a single nucleotide , stimulation of FEN 1 activity by APE 1 resumes . ^^^ Furthermore , APE 1 was able to enhance overall product formation in reconstitution of BER steps involving FEN 1 cleavage followed by ligation . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Estimates based on conserved residues in prokaryote cell septation protein , FtsZ , and proteins involved with synthesis , transcription and replication of DNA revealed FtsZ , ribonucleotide reductase , RNA polymerase core subunits and 5 ' > 3 ' flap exonuclease , FEN 1 , originated soon after cells putatively evolved . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
RNA primer removal from Okazaki fragments during lagging strand replication and the excision of damaged DNA bases requires the action of structure specific nucleases , such as the mammalian flap endonuclease 1 ( FEN 1 ) . ^^^ Unique among these proteins , the putative FEN 1 homologue in Escherichia coli is contained within the N terminal region of the DNA polymerase 1 ( PolN ) . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Recently we reported a novel interaction of the WRN gene product with human 5 ' flap endonuclease / 5 ' 3 ' exonuclease ( FEN 1 ) , a DNA structure specific nuclease implicated in pathways of DNA metabolism that are important for genomic stability . ^^^ WRN enhanced the efficiency of FEN 1 cleavage rather than DNA substrate binding . ^^^ WRN effectively stimulated FEN 1 cleavage on a flap DNA substrate with streptavidin bound to the terminal 3 ' nucleotide at the end of the upstream duplex , indicating that WRN does not require a free upstream end to stimulate FEN 1 cleavage of the 5 ' flap substrate . ^^^ To understand the potential importance of the WRN FEN 1 ( 1 ) interaction in DNA replication , we have tested the effect of WRN on FEN 1 cleavage of several DNA substrate intermediates that may arise during Okazaki fragment processing . ^^^ The ability of WRN to facilitate FEN 1 cleavage of DNA replication / repair intermediates may be important for the role of WRN in the maintenance of genomic stability . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The flexible loop of human FEN 1 endonuclease is required for flap cleavage during DNA replication and repair . ^^^ The conserved , structure specific flap endonuclease FEN 1 cleaves 5 ' DNA flaps that arise during replication or repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Consequently , in the absence of FEN 1 , Pol delta exonuclease activity was essential for closure of simple gaps by DNA ligase . ^^^ Maturation was efficient in the absence of Dna 2 and required Dna 2 only when FEN 1 activity was compromised . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Distribution of functions between FEN 1 AND DNA 2 . ^^^ The Dna 2 nuclease / helicase alone did not efficiently promote nick translation , nor did it affect nick translation with FEN 1 . ^^^ Downstream DNA primers , RNA primers , and small 5 ' flaps were efficiently matured by Pol delta and FEN 1 , and Dna 2 did not stimulate maturation . ^^^ However , maturation of long 5 ' flaps to which replication protein A can bind required both DNA 2 and FEN 1 . ^^^ The maturation kinetics were optimal with a slight molar excess over DNA of Pol delta , FEN 1 , and proliferating cell nuclear antigen . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a nuclear enzyme involved in DNA metabolism , such as replication , repair , and recombination . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Excision of misincorporated ribonucleotides in DNA by RNase H ( type 2 ) and FEN 1 in cell free extracts . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Photoaffinity modification was employed in studying the interaction of the replication protein A ( RPA ) and flap endonuclease 1 ( FEN 1 ) with DNA duplexes similar to structures arising during long patch base excision repair . ^^^ Various DNA substrates were used to study the effects of RPA and FEN 1 on Pol beta mediated DNA synthesis with displacement of a downstream primer . ^^^ In contrast to FEN 1 , RPA had no effect on DNA repair synthesis by Pol beta during long patch base excision repair . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Decreased DNA repair synthesis observed in PARP 1 deficient cell extracts is associated with reduced cellular expression of several factors required for long patch base excision repair ( BER ) , including FEN 1 and DNA ligase I . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Human FEN 1 can process the 5 ' flap DNA of CTG / CAG triplet repeat derived from human genetic diseases by length and sequence dependent manner . ^^^ We demonstrate here that FEN 1 processes the 5 ' flap DNA of CTG / CAG repeats , which is dependent on the length in vitro . ^^^ FEN 1 protein can cleave the 5 ' flap DNA containing triplet repeating sequence up to 21 repeats , but the activity decreases with increasing size of flap above 11 repeats . ^^^ In addition , FEN 1 processing of 5 ' flap DNA depends on sequence , which play a role in the replication origin dependent TNR instability . ^^^ Interestingly , FEN 1 can cleave the 5 ' flap DNA of CTG repeats better than CAG repeats possibly through the flap structure . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The PCNA heterotrimer , but not individual subunits , stimulates the activities of the DNA polymerase , DNA ligase 1 , and the flap endonuclease ( FEN 1 ) of S . solfataricus . ^^^ Distinct PCNA subunits contact DNA polymerase , DNA ligase , or FEN 1 , imposing a defined architecture at the lagging strand fork and suggesting the existence of a preformed scanning complex at the fork . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) , involved in the joining of Okazaki fragments , has been proposed to restrain DNA repeat sequence expansion , a process associated with aging and disease . ^^^ Reconstituting triplet repeat expansion in vitro , we previously found that DNA ligase 1 promotes expansion , but FEN 1 prevents the ligation that forms expanded products . ^^^ However , even wild type FEN 1 exonuclease can not compete with DNA ligase 1 to degrade a bubble structure before it can be ligated . ^^^ A model is presented describing the roles of DNA structure , DNA ligase 1 , and FEN 1 in sequence expansion . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Acetylation occurs at four lysines located at the C terminus of Fen 1 , which is important for DNA binding . ^^^ Taken together , our results confirm the double flap substrate as the likely in vivo intermediate for human Fen 1 and that the C terminal lysines are important for the endonuclease and exonuclease activities likely through DNA binding . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Rates were also elevated in the DNA nuclease deficient strains rad 2 ( defective in the FEN 1 homologue ) and exo 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a structure specific nuclease that removes 5 ' overhanging flaps in DNA repair and replication . ^^^ FEN 1 binds proliferating cell nuclear antigen ( PCNA ) , a DNA clamp protein , when processing Okazaki fragments during lagging strand DNA synthesis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We have previously shown that replication protein A ( RPA ) , the heterotrimeric single stranded DNA binding protein of eukaryotes , plays a role in Okazaki fragment processing by acting as a molecular switch between the two endonucleases , Dna 2 and Fen 1 , to ensure the complete removal of primer RNAs in Saccharomyces cerevisiae . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The dna 2 1 mutant is defective in a helicase nuclease that is required either to coordinate with the crucial Saccharomyces cerevisiae ( sc ) FEN 1 nuclease in Okazaki fragment maturation or to compensate for scFEN 1 when its activity is impaired . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Here we report that , instead of DNA polymerase delta / epsilon , flap endonuclease 1 ( FEN 1 ) , an enzyme involved in base excision repair , is responsible for the formation of double strand DNA break in the assay . ^^^ Because FEN 1 is an essential enzyme that plays its roles in DNA repair and DNA replication , DSBs may be produced in cells as by products of the activity of FEN 1 . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
CRN 1 , a Caenorhabditis elegans FEN 1 homologue , cooperates with CPS 6 / EndoG to promote apoptotic DNA degradation . ^^^ We show that crn 1 , a Caenorhabditis elegans homologue of human flap endonuclease 1 ( FEN 1 ) that is normally involved in DNA replication and repair , is also important for apoptosis . ^^^ Our results suggest that CRN 1 / FEN 1 may play a critical role in switching the state of cells from DNA replication / repair to DNA degradation during apoptosis . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Cyclin dependent kinase ( Cdk ) Cdk 1 Cyclin A can phosphorylate Flap endonuclease 1 ( Fen 1 ) , a key enzyme of the DNA replication machinery , in late S phase . ^^^ As a functional consequence of phosphorylation by Cdk 1 Cyclin A in vitro , endo and exonuclease activities of Fen 1 are reduced whereas its DNA binding is not affected . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Early immobilization of nuclease FEN 1 and accumulation of hRAD 18 protein at stalled DNA replication forks in mammalian cells . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) has been shown to remove 5 ' overhanging flap intermediates during base excision repair and to process the 5 ' ends of Okazaki fragments during lagging strand DNA replication in vitro . ^^^ Fen 1 ( / ) blastocysts fail to form inner cell mass during cellular outgrowth , and a complete inactivation of DNA synthesis in giant cells of blastocyst outgrowth was observed . ^^^ Exposure of Fen 1 ( / ) blastocysts to gamma radiation caused extensive apoptosis , implying an essential role for FEN 1 in the repair of radiation induced DNA damage in vivo . ^^^ Our data thus provide in vivo evidence for an essential function of FEN 1 in DNA repair , as well as in DNA replication . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In cells with no endogenous defects in DNA repair , exogenous nuclease defective FEN 1 causes repeat instability and aberrant DNA repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Removal of the RNA of the RNA DNA junction was brought about by Pf FEN 1 after Pf RNase HII digestion . ^^^ It is likely that Pf RNase HII and Pf FEN 1 cooperatively process Okazaki fragment during lagging strand DNA replication . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The formation of complexes of flap endonuclease 1 ( FEN 1 ) with flapped DNA was shown by photoaffinity modification and gel retardation assays . ^^^ Products of DNA photoattachment to FEN 1 were observed in both cases , while the covalent adducts with RPA were obtained only with the 21 nucleotide long flap . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Using a reconstituted system including the recombinant BER proteins Pol beta , AP endonuclease 1 ( APE 1 ) , 10 ray repair cross complementing group 1 ( XRCC 1 ) , DNA ligase 3 ( Lig 3 ) , flap endonuclease 1 ( FEN 1 ) , and poly ( ADP ribose ) polymerase 1 ( PARP 1 ) , it is demonstrated that in the absence of ATP , both long patch DNA synthesis by Pol beta and poly ( ADP ribosylation ) catalysed by PARP 1 are stimulated . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
This pseudogene is amplified from cDNA preparations contaminated with genomic DNA and must be taken into account in any FEN 1 mutation analysis studies . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
WRN helicase and FEN 1 form a complex upon replication arrest and together process branchmigrating DNA structures associated with the replication fork . ^^^ These results provide evidence for an interaction between WRN and FEN 1 in vivo and suggest that these proteins function together to process DNA structures associated with the replication fork . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In the current model of Okazaki fragment maturation , displacement of a 27 nucleotide or longer flap is envisioned to attract replication protein A ( RPA ) , which inhibits flap endonuclease 1 ( FEN 1 ) but stimulates Dna 2 nuclease for cleavage . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Structural basis for FEN 1 substrate specificity and PCNA mediated activation in DNA replication and repair . ^^^ Flap EndoNuclease 1 ( FEN 1 ) and the processivity factor proliferating cell nuclear antigen ( PCNA ) are central to DNA replication and repair . ^^^ To clarify the molecular basis of FEN 1 specificity and PCNA activation , we report here structures of FEN 1 : DNA and PCNA : FEN 1 peptide complexes , along with fluorescence resonance energy transfer ( FRET ) and mutational results . ^^^ FEN 1 binds the unpaired 3 ' DNA end ( 3 ' flap ) , opens and kinks the DNA , and promotes conformational closing of a flexible helical clamp to facilitate 5 ' cleavage specificity . ^^^ Ordering of unstructured C terminal regions in FEN 1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN 1 and suggests how PCNA stimulates FEN 1 activity . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In vitro , FEN 1 can inhibit TNR expansion by employing its endonuclease activity to compete with DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The Fen 1 extrahelical 3 ' flap pocket is conserved from archaea to human and regulates DNA substrate specificity . ^^^ Very recent structural data obtained from Archaeoglobus fulgidus Fen 1 suggest that an extrahelical 3 ' flap pocket is responsible for substrate specificity , by binding to the unpaired 3 ' flap and by opening and kinking the DNA . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
To investigate the role of WRN in replication , we examined its ability to rescue cellular phenotypes of a yeast dna 2 mutant defective in a helicase endonuclease that participates with flap endonuclease 1 ( FEN 1 ) in Okazaki fragment processing . ^^^ WRN and yeast FEN 1 were reciprocally co immunoprecipitated from extracts of transformed dna 2 1 cells . ^^^ A physical interaction between yeast FEN 1 and WRN is demonstrated by yeast FEN 1 affinity pull down experiments using transformed dna 2 1 cells extracts and by ELISA assays with purified recombinant proteins . ^^^ Collectively , the results suggest that the WRN FEN 1 interaction is biologically important in DNA metabolism and are consistent with a role of the conserved non catalytic domain of a human RecQ helicase in DNA replication intermediate processing . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
This study involves the crystallization and preliminary analysis of the flap endonuclease 1 ( FEN 1 ) DNA repair enzyme from the crenarchaeal organism Aeropyrum pernix ( Ape ) . ^^^ Ape FEN 1 protein in a standard chromatography buffer had only a modest solubility and minimal success in crystallization trials . ^^^ The native Ape FEN 1 crystals diffract to 1 . 4 A resolution and belong to space group P 6 ( 1 ) , with unit cell parameters a = b = 92 . 8 , c = 80 . 9 A , alpha = beta = 90 , gamma = 120 degrees . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a key enzyme involved in base excision repair ( BER ) , a primary pathway utilized by mammalian cells to repair DNA damage . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Processing and joining of DNA ends coordinated by interactions among Dnl4 / Lif1 , Pol 4 , and FEN 1 . ^^^ Genetic studies in Saccharomyces cerevisiae have implicated the DNA polymerase Pol 4 and the DNA structure specific endonuclease FEN 1 ( Rad 27 ) in the processing of DNA ends to be joined by Dnl4 / Lif1 . ^^^ In this study , we demonstrated that FEN 1 ( Rad 27 ) physically and functionally interacted with both Pol 4 and Dnl4 / Lif1 and that together these proteins coordinately processed and joined DNA molecules with incompatible 5 ' ends . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Intact pZ 189 DNA was allowed to replicate in FL FEN 1 ( ) cell line that was established in this laboratory in which the expression of FEN 1 gene was blocked by dexamethasone inducible expression of antisense RNA to FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Efficient nick processing during Okazaki fragment maturation requires the coordinated action of DNA polymerase delta ( Pol delta ) and the FLAP endonuclease FEN 1 . ^^^ If FEN 1 is absent or not optimally functional , the ability of Pol delta to back up via its 3 ' 5 ' exonuclease activity , a process called idling , maintains the polymerase at a position that is ideal either for ligation ( in case of a DNA DNA nick ) or for subsequent engagement by FEN 1 ( in case of a DNA RNA nick ) . ^^^ Consistent with the hypothesis that DNA polymerase epsilon is the leading strand enzyme , we observed no idling by this enzyme and no cooperation with FEN 1 for creating a ligatable nick . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a structure specific nuclease involved both in removing initiator RNA from Okazaki fragments and in DNA repair pathways . ^^^ FEN 1 activity is stimulated by proliferating cell nuclear antigen ( PCNA ) , a toroidal sliding clamp that acts as a platform for DNA replication and repair complexes . ^^^ Like PCNA stimulation , 9 1 1 stimulation can not circumvent the tracking mechanism by which FEN 1 enters the substrate ; however , 9 1 1 does not substitute for PCNA in the stimulation of DNA polymerase beta . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
DNA polymerase beta ( pol beta ) and flap endonuclease 1 ( FEN 1 ) are key players in pol beta mediated long patch base excision repair ( LP BER ) . ^^^ It was proposed that this type of LP BER is accomplished through FEN 1 removal of a 2 to 11 nucleotide flap created by pol beta strand displacement DNA synthesis . ^^^ We observed that nicked DNA and nicked THF flap DNA were poor substrates for pol beta mediated DNA synthesis ; yet , DNA synthesis was strongly stimulated by purified human FEN 1 . ^^^ FEN 1 cleavage activity was required for the stimulation , suggesting that FEN 1 removed a barrier to pol beta DNA synthesis . ^^^ In addition , FEN 1 cleavage on both nicked and nicked THF flap DNA resulted in a one nucleotide gapped DNA molecule that was an ideal substrate for pol beta . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We solved the structure of hPCNA bound to PIP box containing peptides from the p 66 subunit of the human replicative DNA polymerase delta ( 452 466 ) at 2 . 6 A and of the flap endonuclease ( FEN 1 ) ( 331 350 ) at 1 . 85 A resolution . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) participates in removal of RNA primers of Okazaki fragments , several DNA repair pathways , and genome stability maintenance . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Replication protein A facilitates FEN 1 interaction with DNA bubble structures . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a key enzyme involved in base excision repair ( BER ) , a primary pathway utilized by mammalian cells to repair DNA damage . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In the presence of the flap endonuclease FEN 1 , Pol delta rapidly hands off the strand opened product for cutting by FEN 1 , while in its absence , the ability of DNA polymerase delta to switch to its 3 ' > 5 ' exonuclease domain in order to degrade back to the nick position is important in maintaining a ligatable nick . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The yeast RAD 27 gene encodes a functional homolog of the mammalian FEN 1 protein , a structure specific endo / exonuclease which plays an important role in DNA replication and repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Here , we used the FRAP assay to study the dynamics of the GFP tagged PCNA binding proteins : Flap endonuclease 1 ( Fen 1 ) and DNA polymerase eta ( Pol eta ) . ^^^ The decrease of the mobile fraction of focal GFP Fen 1 after DNA damage suggests that Fen 1 exchange depends on the rate of movement of replication forks . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Loading of the proliferating cell nuclear antigen , PCNA , dissociates DNA polymerase ca and recruits DNA polymerase S and the flap endonuclease FEN 1 for elongation and in preparation for its requirement during maturation , respectively . ^^^ Nick translation by the strand displacement action of DNA polymerase 8 , coupled with the nuclease action of FEN 1 , results in processive RNA degradation until a proper DNA nick is reached for closure by DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The multiple functions of FEN 1 are regulated via several means , including formation of complexes with different protein partners , nuclear localization in response to cell cycle or DNA damage and post translational modifications . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 ( FEN 1 ) is a structure specific nuclease involved in DNA replication and repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We have used a combination of in situ extraction and dual color photobleaching to compare the dynamic properties of three proteins essential for lagging strand synthesis : the polymerase clamp proliferating cell nuclear antigen ( PCNA ) and two proteins that bind to it , DNA Ligase 1 and Fen 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In the present work , the influence of several BER proteins such as flap endonuclease 1 ( FEN 1 ) , PCNA , and apurinic / apyrimidinic endonuclease 1 ( APE 1 ) on the activity of Pol lambda was investigated . ^^^ FEN 1 processes nicked DNA , thus removing a barrier to Pol lambda DNA synthesis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Fen 1 facilitates homologous recombination by removing divergent sequences at DNA break ends . ^^^ To clarify whether sequence divergence at DNA ends is truly the reason for the observed HR defect in FEN 1 ( / ) cells we inserted a unique 1 SceI restriction site in the genome and tested various donor and recipient HR substrates . ^^^ We conclude that Fen 1 eliminates heterologous sequences at DNA damage site and facilitates DNA repair by HR . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
An enzyme involved in processing stalled DNA replication forks is flap endonuclease 1 ( Fen 1 ) . ^^^ Transgenic expression of Fen 1 in p 53 null cells attenuated UV C light induced DNA replication inhibition , supporting the hypothesis that Fen 1 induction is involved in the recovery of cells from DNA damage . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Partial base excision DNA repair ( BER ) reconstituted with purified enzymes demonstrated that Flap endonuclease 1 ( FEN 1 ) efficiently excises a displaced oligonucleotide containing a 5 ' terminal dL residue , as would be produced during long patch ( multinucleotide ) BER . ^^^ Simultaneous monitoring of dL repair and dL mediated DPC formation demonstrated that removal of the dL residue through the combined action of strand displacement DNA synthesis by polbeta and excision by FEN 1 markedly diminished DPC formation with the polymerase . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Biochemical and genetic data indicate that an important protein interaction of WRN and Bloom syndrome ( BLM ) helicases is with the structure specific nuclease Flap Endonuclease 1 ( FEN 1 ) , an enzyme that is implicated in the processing of DNA intermediates that arise during cellular DNA replication , repair and recombination . ^^^ These studies provide new insights to the interaction of WRN and BLM helicases with FEN 1 , and how these interactions might be regulated with the PCNA FEN 1 interaction during DNA replication and repair . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
These functions include loading onto DNA by replication factor C , as well as Okazaki fragment synthesis and maturation by the PCNA coordinated actions of DNA polymerase delta , the flap endonuclease FEN 1 , and DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Using a screen for potential interactions between telomere repeat binding factor 2 ( TRF 2 ) and proteins involved in BER of oxidized bases in vitro , we found that TRF 2 physically bound DNA polymerase beta ( Pol beta ) and flap endonuclease 1 ( FEN 1 ) . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Thus , DNA duplexes with modifications in sugar phosphate backbone can be used to mimic intermediates of the long patch pathway of BER in reconstituted systems containing FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Damage specific association of condensin 1 with the BER factors flap endonuclease 1 ( FEN 1 ) and DNA polymerase delta / epsilon was also observed , suggesting that condensin 1 is recruited to interact with BER factors at damage sites . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The DNA protein interaction modes of FEN 1 with gap substrates and their implication in preventing duplication mutations . ^^^ Recently , FEN 1 has been reported to also possess a gap endonuclease ( GEN ) activity , which is possibly involved in apoptotic DNA fragmentation and the resolution of stalled DNA replication forks . ^^^ In the current study , we compare the kinetics of these activities to shed light on the aspects of DNA structure and FEN 1 DNA binding elements that affect substrate cleavage . ^^^ By using DNA binding deficient mutants of FEN 1 , we determine that the GEN activity is analogous to FEN activity in that the single stranded DNA region of DNA substrates interacts with the clamp region of FEN 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In eukaryotes , the creation of ligatable nicks in DNA from flap structures generated by DNA polymerase delta catalyzed displacement DNA synthesis during Okazaki fragment processing depends on the combined action of Fen 1 and Dna 2 . ^^^ Dna 2 is well suited to process long flaps , which are converted to nicks by the subsequent action of Fen 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Yeast Dna 2 helicase / nuclease is essential for DNA replication and assists FEN 1 nuclease in processing a subset of Okazaki fragments that have long single stranded 5 ' flaps . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Under the same conditions , these proteins inhibited strand displacement DNA synthesis and decreased the efficiency of the flap endonuclease 1 ( FEN 1 ) catalyzed endonuclease reaction in the nuclear extract , blocking repair of DNA duplex by the long patch subpathway . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Based on these findings , we propose that Fen 1 , despite its role in DNA repair and replication , is not primarily involved in maintaining stability at the DM 1 locus . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We have isolated alleles of RAD27 / ERC11 / YKL510 , the yeast homolog of the gene encoding flap endonuclease 1 , FEN 1 . cln1 cln 2 rad27 / erc11 cells arrest in S phase ; this cell cycle arrest is suppressed by the expression of CLN 1 or CLN 2 but not by that of CLN 3 or the hyperactive CLN 3 2 . rad27 / erc11 mutants are also defective in DNA damage repair , as determined by their increased sensitivity to a DNA damaging agent , increased mitotic recombination rates , and increased spontaneous mutation rates . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Mechanism of tracking and cleavage of adduct damaged DNA substrates by the mammalian 5 ' to 3 ' exonuclease / endonuclease RAD 2 homologue 1 or flap endonuclease 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Human flap endonuclease 1 ( hFEN 1 ) is highly homologous to human XPG , Saccharomyces cerevisiae RAD 2 and S . cerevisiae RTH 1 and shares structural and functional similarity with viral exonucleases such as T 4 RNase H , T 5 exonuclease and prokaryotic DNA polymerase 5 ' nucleases . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 , which preferentially cleaves unannealed 5 ' flap structures in DNA , has been shown to play a crucial role in the long patch mode of repair . ^^^ We reconstituted the final steps of long patch base excision repair in vitro using calf DNA polymerase epsilon to provide strand displacement synthesis , human flap endonuclease 1 , and human DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
This result indicates a back up function of exonuclease 1 to flap endonuclease 1 in RNA primer removal during lagging strand DNA synthesis . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Our data indicated that efficient repair is dependent on six components including AP endonuclease , replication factor C , proliferating cell nuclear antigen , DNA polymerases delta or epsilon , flap endonuclease 1 , and DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Finally , a combination of HIV 1 RT , Flap endonuclease 1 , and DNA ligase is capable of quantitatively forming covalently closed DNA with these model substrates . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Interaction between human flap endonuclease 1 ( hFEN 1 ) and proliferating cell nuclear antigen ( PCNA ) represents a good model for interactions between multiple functional proteins involved in DNA metabolic pathways . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Molecular structure and novel DNA binding sites located in loops of flap endonuclease 1 from Pyrococcus horikoshii . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
While the combined treatment induced a synergistic effect on the expression of LCK proto oncogene and several genes related to protein synthesis / processing , a negative interaction effect was found for the expression of genes related to cytoskeleton and extracellular matrix assembly ( SATB 1 and Anexin 3 ) , cell cycle control ( tyrosine kinase ) , and genes participating in DNA / RNA synthesis and repair ( RNA helicase , FLAP endonuclease 1 , DNA 3 glycosylase methyladenine , splicing factor SC 35 and Soh 1 ) . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The phosphorylation of proteins such as replication protein A , DNA polymerase alpha and delta , replication factor C , flap endonuclease 1 and DNA ligase 1 leads to their inactivation , suggesting that phosphorylation is important in the prevention of re replication . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Triplet repeat expansion generated by DNA slippage is suppressed by human flap endonuclease 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Flap endonuclease 1 : a central component of DNA metabolism . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The very recently described interactions of Rad 9 Rad1 Hus 1 with MutY DNA glycosylase , DNA polymerase beta and Flap endonuclease 1 now complete our view that the long patch base excision machinery is an important target of the Rad 9 Rad1 Hus 1 complex , thus enhancing the quality control of DNA . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The proteins and enzymatic activities that were found to copurify with the NB DNA synthesome include : DNA polymerases alpha , delta , and epsilon , proliferating cell nuclear antigen , replication factor A , replication factor C , topoisomerases 1 and 2 , flap endonuclease 1 , and DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The two DNA clamps Rad9 / Rad1 / Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
It binds the DNA glycosylase MutY homolog , and stimulates DNA polymerase beta , flap endonuclease 1 , and DNA ligase 1 . 9 1 1 resembles proliferating cell nuclear antigen ( PCNA ) , which stimulates some of these same repair enzymes , and is loaded onto DNA in a similar manner . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Methanosarcina acetivorans flap endonuclease 1 activity is inhibited by a cognate single stranded DNA binding protein . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
RESULTS AND CONCLUSIONS : After 12 h of treatment , in parallel with the 1 , 25 ( OH ) 2D3 induced G 1 arrest , a particular set of DNA replication genes including a cell division cycle 6 homolog , a DNA polymerase alpha subunit , proliferating cell nuclear antigen , two DNA polymerase delta subunits , and flap structure specific endonuclease 1 , was downregulated at least 2 fold . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Using these proteins , complete SV 40 DNA replication was reconstituted with only purified DNA replication factors : SV 40 large tumor antigen ( TAg ) , replication protein A ( RPA ) , DNA topoisomerases 1 and 2 , DNA polymerase alpha primase , replication factor C ( RFC ) , the proliferating cell nuclear antigen ( PCNA ) , DNA polymerase delta , maturation factor 1 ( MF 1 ) , and DNA ligase 1 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
MX , giving apex as a homozygote , is dominant to MF 1 , giving frenata , which is dominant to the other alleles . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Hence , we used the electrophoretic mobility shift assay to compare the effect of T 3 on the DNA binding of mutant TR beta 1 ( Mf 1 ) from a kindred with GRTH with normal TR beta . ^^^ Mf 1 bound better as a homodimer than TR beta , but dissociated from DNA only at high T 3 concentrations . ^^^ Surprisingly , T 3 disrupted the DNA binding of the Mf 1 / TR isoform dimers . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The murine Mf 1 and Mfh 1 genes have overlapping patterns of expression in the embryo and encode forkhead / winged helix transcription factors with virtually identical DNA binding domains . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
By means of this MF 1 procedure , apoptotic cells exhibited characteristic changes of light scatter ( size ) and fluorescence ( DNA content ) relating to cell shrinkage and nuclear fragmentation of apoptosis . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Six DNA samples obtained from non founding migraine affected members of migraine family 1 ( MF 1 ) were used in this study . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The genes RAD 1 , RAD 2 , RAD 3 and RAD 4 encode enzymes in the pathway leading to excision repair of UV induced DNA damage in Saccharomyces cerevisiae . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Regulation of the yeast RAD 2 gene : DNA damage dependent induction correlates with protein binding to regulatory sequences and their deletion influences survival . ^^^ In the yeast Saccharomyces cerevisiae the RAD 2 gene is absolutely required for damage specific incision of DNA during nucleotide excision repair and is inducible by DNA damaging agents . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Repair synthesis of UV irradiated plasmid DNA was observed in a radiation dose dependent manner and was unaffected by mutations in the RAD 1 , RAD 2 , RAD 3 , RAD 4 , RAD 10 , or APN 1 genes . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
RAD 18 resembles the other DNA repair genes , RAD 2 , RAD 6 , RAD 7 , RAD 23 , and RAD 54 , all of which also exhibit increased transcription in response to DNA damage and during meiosis . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The Saccharomyces cerevisiae DNA repair gene RAD 2 is regulated in meiosis but not during the mitotic cell cycle . ^^^ The expression of the RAD 2 gene of Saccharomyces cerevisiae is elevated upon DNA damage . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The number of induced diadducts was verified by determination of interstrand cross links via denaturation and renaturation of 8 MOP + UVA treated DNA from RAD and rad 2 yeast strain . 8 MOP + UVA was shown to induce two types of locus specific mutations : reversion of the lys 1 1 ochre allele was between 20 to 50 fold higher than that of the his 4 38 frameshift allele . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Two Saccharomyces cerevisiae genes necessary for excision repair of UV damage in DNA , RAD 1 and RAD 2 , were introduced individually , on a yeast shuttle vector , into seven Schizosaccharomyces pombe mutants rads 1 , 2 , 5 , 13 , 15 , 16 and 17 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Mutations in the RAD 1 , RAD 2 , RAD 3 , RAD 4 and RAD 10 genes render cells highly defective in the incision of damaged DNA , whereas mutations in the RAD 7 , RAD 14 , RAD 16 , RAD 23 and MMS 19 genes reduce the level of damage excision . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Following induction we observed a time dependent methylation of yeast DNA in RAD+ and rad 2 mutant strains ; the rad 2 mutant is defective in excision repair of UV induced DNA damage . ^^^ Analysis of restriction endonuclease digestion patterns revealed that the relative amount of methylated DNA was greater in the excision defective rad 2 mutant than in the RAD+ strain . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Regulation of the DNA damage inducible RAD 2 gene was investigated in yeast cells transformed with centromeric plasmids containing RAD 2 lacZ fusion constructs . ^^^ We therefore conclude that induction of RAD 2 by DNA damaging agents is positively regulated . ^^^ However , meiosis is accompanied by increased steady state levels of RAD 2 mRNA in the absence of DNA damage . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
No amplified DNA was detected in RAD 2 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The RAD 1 , RAD 2 , RAD 4 and RAD 14 genes of the RAD 3 epistasis group are implicated in the repair of ethylations to DNA . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Repair of plasmid DNA treated with 8 methoxypsoralen and long wave UV light ( lambda=365 nm ) in wild type and mutant rad 2 cells of Saccharomyces cerevisiae ] . ^^^ The method of repeated irradiation has been used to study excision of 8 MOP monoadducts from plasmid and chromosomal DNA in cells of wild type and rad 2 mutant of Saccharomyces cerevisiae . ^^^ The measurement of kinetics of monoadduct removal from chromosomal DNA in intact and competent yeast cells showed that monoadducts were excised in both types of cells with normal repair , but this process was blocked in intact and competent cells of the rad 2 mutant . ^^^ It was shown that monoadducts were removed equally effectively from plasmid DNA introduced into cells of the wild type and rad 2 mutant . ^^^ Possibly , the repair system of both these strains provides excision of monoadducts from plasmid DNA , but this process is blocked in the rad 2 mutant , relatively to monoadduct excision from chromosomal DNA . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The repair of in vitro UV irradiated DNA of plasmid pBB 29 was studied in excision defective yeast mutants rad 1 , rad 2 , rad 3 , rad 4 , rad 10 and in Escherichia coli mutants uvr and recA , by measuring the cell transformation frequency . ^^^ Rad 2 , rad 3 , rad 4 , and rad 10 mutants could repair plasmid DNA despite their inability to repair nuclear DNA , whereas the reduced ability of rad 1 mutant for plasmid DNA repair demonstrated alone the same dependence on the host functions that are needed for nuclear DNA repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We suppose that the damages of DNA induced by QM in the wild type cells can be excised , but in the rad 2 cells the gaps in DNA appeared after replication . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Hyperthermia leads mainly to a lengthening of G 1 , whereas 10 rays arrest cells of the rad 2 and rad 9 mutant in G 2 and the rad 51 mutant additionaly in a state with DNA contents above G 2 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
A plasmid ( pNF 2000 ) containing a 9 . 7 kilobase pair DNA insert that complements the UV sensitivity of rad 2 1 , rad 2 2 , and rad 2 4 mutants of Saccharomyces cerevisiae has been isolated from a yeast genomic library . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
With rad 1 , rad 2 , rad 3 , rad 4 , and rad 10 mutants , the molecular weight of the DNA remained unchanged after ultraviolet irradiation and incubation at the restrictive temperature , despite the presence of the cdc 9 mutation ; these mutants are therefore incision defective . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The ERCC 5 cDNA encodes a predicted 133 kDa nuclear protein that shares some homology with the product of the yeast DNA repair gene RAD 2 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae RAD 2 protein and its human homolog xeroderma pigmentosum group G ( XPG ) protein function in the incision step of nucleotide excision repair of DNA damaged by ultraviolet light . ^^^ Using DNA substrates labeled at either the 5 ' end or 3 ' end , we now demonstrate that RAD 2 protein also digests both single stranded and double stranded DNAs exonucleolytically with a 5 ' to 3 ' directionality . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad 2 gene , which is required for chromosome segregation and recovery from DNA damage . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Rad 2 ( a single stranded DNA endonuclease ) specifically interacts with the Tfb 1 subunit of factor b , and we have mapped a limited region of the Rad 2 polypeptide which is sufficient for this interaction . ^^^ Rad 2 also interacts directly with Ss 12 protein ( a putative DNA helicase ) . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Yeast excision repair gene RAD 2 encodes a single stranded DNA endonuclease . ^^^ Here we overproduce the RAD 2 encoded protein in S . cerevisiae , purify it to near homogeneity , and show that RAD 2 protein in the presence of magnesium degrades circular single stranded DNA . ^^^ The RAD 2 endonuclease is specific for single stranded DNA as it does not act on double stranded DNA . ^^^ Given the absolute requirement for RAD 2 in the incision step of excision repair , our findings directly implicate RAD 2 protein and its human homologue XPG protein as a catalytic component that incises the damaged DNA strand during excision repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The SPK 1 upstream region also includes a domain highly homologous to sequences involved in induction of RAD 2 and other excision repair genes by agents that induce DNA damage . spk 1 strains were hypersensitive to UV irradiation . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Complementation of the DNA repair defect in xeroderma pigmentosum group G cells by a human cDNA related to yeast RAD 2 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae Rad 2 protein functions in the incision step of the nucleotide excision repair of DNA damaged by ultraviolet light . ^^^ Rad 2 was previously shown to act endonucleolytically on circular single stranded M 13 DNA and also to have a 5 ' > 3 ' exonuclease activity ( Habraken , Y . , Sung , P . , Prakash , L . , and Prakash , S . ( 1993 ) Nature 366 , 365 368 ; Habraken , Y . , Sung , P . , Prakash , L . , and Prakash , S . ( 1994 ) J . ^^^ Using two different branched DNA structures , pseudo Y and flap , we have determined that Rad 2 specifically cleaves the 5 ' overhanging single strand in these DNAs . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Structure of bacteriophage T 4 RNase H , a 5 ' to 3 ' RNA DNA and DNA DNA exonuclease with sequence similarity to the RAD 2 family of eukaryotic proteins . ^^^ Bacteriophage T 4 RNase H is a 5 ' to 3 ' exonuclease that removes RNA primers from the lagging strand of the DNA replication fork and is a member of the RAD 2 family of eukaryotic and prokaryotic replication and repair nucleases . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The role of the exonucleolytic activity of the calf 5 ' to 3 ' exo / endonuclease , a RAD 2 homolog 1 ( RTH 1 ) class nuclease , in lagging strand DNA replication has been examined using model Okazaki fragment substrates . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In the early embryo , the maternally supplied tos mRNA is evenly distributed at the syncytial blastoderm stage , but is excluded from the forming cells when cellularization begins . tos product is the first Drosophila member of the RAD 2 protein family , a group of related DNA repair nucleases conserved from yeast to humans . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Transcription factor TFIIH and DNA endonuclease Rad 2 constitute yeast nucleotide excision repair factor 3 : implications for nucleotide excision repair and Cockayne syndrome . ^^^ Neither of the two endonucleases , Rad 1 Rad10 and Rad 2 , required for dual incision , shows any affinity for ultraviolet damaged DNA . ^^^ It has remained unclear how the Rad 2 nuclease is targeted to the DNA damage site and why mutations in the human RAD 2 counterpart , XPG , result in Cockayne syndrome . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Human RAD 2 homolog 1 5 ' to 3 ' exo / endonuclease can efficiently excise a displaced DNA fragment containing a 5 ' terminal abasic lesion by endonuclease activity . ^^^ Eukaryotic RAD 2 homolog 1 ( RTH 1 ) nuclease , by genetic and biochemical evidence , is involved in repair of modified DNA . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Characterization of a novel DNA damage inducible gene of Saccharomyces cerevisiae , DIN 7 , which is a structural homolog of the RAD 2 and RAD 27 DNA repair genes . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Sequence analysis of an 11 , 628 bp DNA segment from the right arm of Saccharomyces cerevisiae chromosome 7 revealed the presence of the 5 ' end of the RAD 2 gene , the MES 1 gene and six open reading frames ( ORFs ) each longer than 300 bp . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
These data provide evidence of a high degree of specificity for the role of RTH 1 in DNA replication and in base excision repair , and for the requirement of RAD 2 in nucleotide excision repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Based on its homology to this and other DNA repair proteins of the Rad 2 family , most notably Schizosaccharomyces pombe exonuclease 1 ( Exo 1 ) , Hex 1 presumably functions as a nuclease in aspects of recombination or mismatch repair . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We report here that the RAD 2 domain of human exonuclease 1 ( HEX 1 N2 ) exhibits both a robust 5 ' to 3 ' exonuclease activity on single and double stranded DNA substrates as well as a flap structure specific endonuclease activity but does not show specific endonuclease activity at 10 base pair bubble like structures , G : T mismatches , or uracil residues . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We find that transposition is elevated in cells mutated for genes in the RAD 52 recombinational repair pathway , such as RAD 50 , RAD 51 , RAD 52 , RAD 54 , or RAD 57 , or in the DNA ligase 1 gene CDC 9 , but is not elevated in cells mutated in the DNA repair functions encoded by the RAD 1 , RAD 2 , or MSH 2 genes . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In addition , S . pombe mutant for the flap endonuclease rad 2 gene , whose precise function in DNA repair is unclear , were also more alkylation sensitive than mag 1 mutants . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
This reaction requires the damage binding factors Rad 14 , RPA , and the Rad 4 Rad23 complex , the transcription factor TFIIH which contains the two DNA helicases Rad 3 and Rad 25 , essential for creating a bubble structure , and the two endonucleases , the Rad 1 Rad10 complex and Rad 2 , which incise the damaged DNA strand on the 5 ' and 3 ' side of the lesion , respectively . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Role of the DNA repair nucleases Rad 13 , Rad 2 and Uve 1 of Schizosaccharomyces pombe in mismatch correction . ^^^ We studied mismatch correction efficiency in cells with inactivated DNA repair nucleases Rad 13 , Rad 2 or Uve 1 in MMR proficient and deficient background . ^^^ Rad 2 has a function in the Uve 1 dependent repair of DNA damages and in replication . ^^^ Thus , the function of Rad 2 in mutation avoidance is rather independent of NER . rad 13 , swi 10 and rad 2 , but not uve 1 mutants were sensitive to the DNA damaging agent methyl methane sulphonate . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
In order to identify elements that dictate substrate selectivity within the RAD 2 family , we sought to identify residues key to Exo 1 nuclease activity and to characterize the molecular details of the human Exo 1 DNA interaction . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
To identify novel genes involved in DNA double strand break ( DSB ) repair , we previously isolated Schizosaccharomyces pombe mutants which are hypersensitive to methyl methanesulfonate ( MMS ) and synthetic lethals with rad 2 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We tested UV induced mutagenesis in double mutants carrying hsm 2 1 mutation and a mutation in a gene of principal damaged DNA repair pathways ( rad 2 and rev 3 ) or in a mismatch repair gene ( pms 1 and recently characterized in our laboratory hsm 3 ) . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
The nth 1 mutant also exhibited elevated frequencies of spontaneous mitotic intrachromosomal recombination , which is a phenotype shared by the MMS hypersensitive DNA repair mutants rad 2 , rhp 55 and NER repair mutants rad 16 , rhp 14 , rad 13 and swi 10 . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Exonuclease 1 ( EXO 1 ) , a member of the RAD 2 family of nucleases , has recently been proposed to function in the genetic pathways of DNA recombination , repair , and replication which are important for genome integrity . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Although deletion of MAG 1 in the base excision repair pathway enhances the detection sensitivity to DNA alkylating agents , and deletion of RAD 2 in the nucleotide excision repair pathway enhances the detection sensitivity to ultraviolet and agents that produce bulky lesions , inactivation of genes involved in the recombination repair and postreplication repair variably reduces RNR 3 lacZ induction . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Rad 2 family nucleases , identified by sequence similarity within their catalytic domains , function in multiple pathways of DNA metabolism . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We suggest that functional interactions between RecQ helicases and Rad 2 family nucleases serve to process DNA substrates that are intermediates in DNA replication and repair . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
A role for yeast and human proteins in DNA repair is suggested by the demonstration that Sub 1 acts in a peroxide resistance pathway involving Rad 2 and by the physical interaction of PC 4 with the human Rad 2 homolog XPG . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
EXO 1 is a member of the RAD 2 nuclease family and functions in DNA replication , repair and recombination . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We tested the induced mutagenesis in double mutants carrying him 1 mutation and mutations in other repair genes : apn 1 , blocking base excision repair ; rad 2 , rev 3 , and rad 54 , blocking three principal DNA repair pathways ; pms 1 , blocking mismatch repair ; hsm 2 and hsm 3 mutations , which lead to a mutator effect . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Genetic and physical interactions between Schizosaccharomyces pombe Mcl 1 and Rad 2 , Dna 2 and DNA polymerase alpha : evidence for a multifunctional role of Mcl 1 in DNA replication and repair . ^^^ Schizosaccharomyces pombe rad 2 is involved in Okazaki fragments processing during lagging strand DNA replication . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
On the basis of the biochemical properties of Rad 2 , we propose that meiotic recombination by this alternative ( Rec * ) pathway can be initiated by non DSB lesions , such as nicks and gaps , which accumulate during premeiotic DNA replication in the absence of Okazaki fragment processing . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
Silencing requires rad 2 , gei 17 , and the polh 1 translesion DNA polymerase , which suppress replication fork stalling and thereby eliminate the checkpoint activating signal . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
We conclude that repair of oxidative DNA damage , such as thymine glycols , can be coupled to transcription and that RAD 2 facilitates transcription coupled repair of oxidative DNA damage in yeast . . ^^^
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P39748 and P27695	Pubmed	SVM Score :0.0
NA