Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.65425539
Measurement of the interaction of a purified alpha / beta type SASP with DNA in vitro by a filter binding assay showed that the binding saturated at one molecule of SASP per approximately 5 bp . 0.65425539^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.65229237
When an alpha / beta type SASP was incubated with linear DNA , the protein bound cooperatively , forming a helical coating 6 . 6 + / 0 . 4 nm wide with a 2 . 9 + / 0 . 3 nm periodicity . alpha / beta Type SASP binding to an 890 bp DNA was weakest at an ( A+T ) rich region that was highly bent , but binding eliminated the bending . alpha / beta Type SASP binding did not alter the rise per bp in DNA but greatly increased the DNA stiffness as measured by both electron microscopic and cyclization assays . 0.65229237^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :1.014034
In both an in vitro diamide induced cross linking study and an in vivo mammalian two hybrid assay we proved that TRX can associate directly with Ref 1 in the nucleus ; also , we demonstrated the requirement of cysteine residues in the TRX catalytic center for the potentiation of AP 1 activity . 1.014034^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.51153935
In the nucleus , however , TRX enhances NF kappaB transcriptional activities by enhancing its ability to bind DNA . 0.51153935^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Using electrophoretic mobility shift assay ( EMSA ) , which demonstrates specific DNA protein interaction in vitro , we could demonstrate that reducto oxidative modulation of NF kappa B dramatically changes its DNA binding activity and that a cellular physiological reducing catalyst , thioredoxin ( TRX ) also known as adult T cell leukemia derived factor ( ADF ) , fully restored the DNA binding activity of the oxidized NF kappa B . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
These HPV DNA positive cells were also positive for ADF . ^^^ Invasive squamous cell carcinoma was also positive for ADF ( 24 / 34 cases ) and HPV DNA ( 11 / 34 cases ) . ^^^ The coexpression of HPV DNA and ADF was observed in all HPV DNA positive cases . ^^^ Coexistence of HPV DNA and ADF immunopositivity in neoplastic squamous cells of the cervix suggests that ADF expression closely reflects the intracellular event on HPV DNA replication . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
As factors regulating growth and differentiation of cervical squamous epithelium , immunohistochemical expression of estrogen receptor ( ER ) , progesterone receptor ( PR ) , epidermal growth factor receptor ( EGFR ) , c erbB 2 protein , adult T cell leukemia derived factor ( ADF ) , and HPV DNA was examined . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
ADF / hTRX can facilitate the interactions between the transcriptional factors and its target DNA sequences , which may result in the overexpression of IL 2R alpha in HTLV 1 infected cells . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Based on a partial N terminal amino acid sequence , complementary DNA ( cDNA ) clones for human and mouse ADF were isolated and sequenced . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
For example , TRX / ADF translocates from the cytosol into the nucleus by a variety of cellular stresses , to regulate the expression of various genes through the redox factor 1 ( Ref 1 ) / APEX . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
CONCLUSIONS : Many of the ADF fatalities who could not be positively identified in the past could be identified today through the use of DNA techniques . ^^^ This paper recommends the establishment of a DNA repository to store reference blood samples to facilitate the identification of ADF aircrew remains without causing additional distress to family members . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We designed , prepared and characterized a prototype of ADF GFP proteins capable of specific interaction with DNA fragments bearing nuclear factor ( NF ) kappaB sites . ^^^ ADF NF kappaB p 50 and a non DNA binding deletion mutant ( p 35 ) combined with GFPuv were inserted in a procaryotic vector and expressed in E . coli . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA in spores of Bacillus and Clostridium species is associated with small , acid soluble proteins ( SASP ) of the alpha / beta type ; the presence of these proteins is a major factor in causing spore resistance to UV light , alpha / beta type SASP did not bind to single stranded DNA , single or double stranded RNA , or DNA RNA hybrids in vitro . ^^^ The binding of alpha / beta type SASP to DNA saturated at a protein / DNA ratio ( wt / wt ) of 4 : 1 to 5 : 1 , which is approximately 1 SASP per 4 bp . alpha / beta type SASP DNA interaction did not require divalent cations , was independent of pH between 6 and 8 , and , for some SASP DNA pairs , was relatively insensitive to salt up to 0 . 3 M . ^^^ This order in alpha / beta type SASP DNA affinities parallels the facility with which the DNAs adopt an A like conformation , the conformation in alpha / beta type SASP DNA complexes . ^^^ In addition to blocking DNase digestion , binding of alpha / beta type SASP to DNA blocked ( 1 ) cleavage of the DNA backbone by hydroxyl radicals and orthophenanthroline Cu2+ , ( 2 ) DNA cleavage by restriction enzymes , in particular those with specificity for GC rich sequences ; and ( 3 ) in vitro transcription of some but not all genes . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Two derivatives of the alpha / beta type small acid soluble spore protein ( SASP ) SspCwt have been constructed , each containing a residue potentially useful for physico chemical analysis of protein protein or protein DNA interactions . ^^^ These data indicate that the two mutant proteins are extremely similar to SspCwt in their interaction with DNA , and thus may be useful in probing SASP SASP and SASP DNA interactions directly by physical or chemical techniques . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The DNA in dormant spores of Bacillus species is associated with alpha / beta type small , acid soluble proteins ( SASP ) , which are double stranded DNA binding proteins whose amino acid sequence has been highly conserved in evolution . ^^^ In vitro these proteins bind most strongly to DNA which readily adopts an A like conformation , as binding of alpha / beta type SASP causes DNA to assume an A like conformation . ^^^ As predicted by this conformational change in DNA , binding of alpha / beta type SASP to relaxed but covalently closed plasmid DNA results in the introduction of a large number of negative supercoils . ^^^ Associated with the conformational change in DNA brought about by alpha / beta type SASP binding is a change in its photochemistry such that ultraviolet irradiation does not generate pyrimidine dimers , but rather a thyminyl thymine adduct termed spore photoproduct ( SP ) . ^^^ The latter two properties of DNA complexed with alpha / beta type SASP in vitro are similar to those of DNA in dormant spores of Bacillus species in which : ( 1 ) plasmid DNA has a much higher number of negative supercoils than plasmid in growing cells ; and ( 2 ) ultraviolet irradiation produces SP and no pyrimidine dimers , while only pyrimidine dimers are formed in growing cells . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Small , acid soluble proteins ( SASP ) of the alpha / beta type from spores of Bacillus and Clostridium species bind to DNA ; this binding prevents formation of cyclobutane type thymine dimers upon UV irradiation , but promotes formation of the spore photoproduct , an adduct between adjacent thymine residues . alpha / beta Type SASP also bound to poly ( dG ) . poly ( dC ) and poly ( dA dG ) . poly ( dC dT ) . ^^^ These data provide further evidence for the dramatic change in DNA structure and photoreactivity which takes place on binding of alpha / beta type SASP and suggest that binding of these proteins to DNA in vivo prevents formation of most deleterious photoproducts upon UV irradiation . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Synthesis and characterization of a 29 amino acid residue DNA binding peptide derived from alpha / beta type small , acid soluble spore proteins ( SASP ) of bacteria . ^^^ A 29 amino acid residue peptide ( SASP peptide ) derived from the sequence of the putative DNA contacting portion at the carboxyl terminus of an alpha / beta type small , acid soluble spore protein ( SASP ) of Bacillus subtilis has been synthesized by automated solid phase methods and tested for its ability to interact with DNA . ^^^ Circular dichroism ( CD ) spectroscopy reveals an interaction between this SASP peptide and DNA , both by an increase in alpha helix content of the peptide ( which alone has a mostly random conformation ) and by enhancement of the 275 nm CD band of the DNA . ^^^ In contrast to results with intact alpha / beta type SASP , however , the peptide does not induce a B A conformational transition in the DNA . ^^^ The results confirm the hypothesis that the carboxyl terminal region of the alpha / beta type SASP directly contacts DNA and possesses some , but not all , of the functional characteristics of the intact molecule . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
These data suggest that ( 1 ) alpha / beta type SASP remain bound to much , although not all , of the chromosome in germinated gpr spores ; ( 2 ) the alpha / beta type SASP bound to the chromosome in gpr spores alter this DNA ' s topology and UV photochemistry ; and ( 3 ) the presence of alpha / beta type SASP on the chromosome is detrimental to normal spore outgrowth . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Small , acid soluble proteins ( SASP ) of the alpha / beta type are associated with DNA in spores of Bacillus subtilis . ^^^ Induction of synthesis of alpha / beta type SASP in Escherichia coli resulted in rapid cessation of DNA synthesis , followed by a halt in RNA and then protein accumulation , although significant mRNA and protein synthesis continued . ^^^ The nucleoids of cells with alpha / beta type SASP were extremely condensed , as viewed in both light and electron microscopes , and immunoelectron microscopy showed that the alpha / beta type SASP were associated with the cell DNA . ^^^ Induction of alpha / beta type SASP synthesis in E . coli increased the negative superhelical density of plasmid DNA by approximately 20 % ; UV irradiation of E . coli with alpha / beta type SASP gave reduced yields of thymine dimers but significant amounts of the spore photoproduct . ^^^ These changes in E . coli DNA topology and photochemistry due to alpha / beta type SASP are similar to the effects of alpha / beta type SASP on the DNA in Bacillus spores , further suggesting that alpha / beta type SASP are a major factor determining DNA properties in bacterial spores . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Effects of mutant small , acid soluble spore proteins from Bacillus subtilis on DNA in vivo and in vitro . alpha / beta type small , acid soluble spore proteins ( SASP ) of Bacillus subtilis bind to DNA and alter its conformation , topology , and photochemistry , and thereby spore resistance to UV light . ^^^ Both SspCwt and SspCTyr interacted similarly with DNA in vivo and in vitro , restoring much UV resistance to spores lacking major alpha / beta type SASP , causing a large increase in plasmid negative supercoiling , and altering DNA UV photochemistry from cell type to spore type . ^^^ These results confirm the importance of the conserved primary sequence of alpha / beta type SASP in the ability of these proteins to bind to spore DNA and cause spore UV resistance . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
UV irradiation of complexes of DNA and an alpha / beta type small , acid soluble protein ( SASP ) from Bacillus subtilis spores gave decreasing amounts of pyrimidine dimers and increasing amounts of spore photoproduct as the SASP / DNA ratio was increased . ^^^ The yields of pyrimidine dimers and spore photoproduct were less than 0 . 2 % and 8 % of total thymine , respectively , when DNA saturated with SASP was irradiated at 254 nm with 30 kJ / m2 ; in the absence of SASP the yields were reversed 4 . 5 % and 0 . 3 % , respectively . ^^^ Complexes of DNA with alpha / beta type SASP from Bacillus cereus , Bacillus megaterium , or Clostridium bifermentans spores also gave spore photoproduct upon UV irradiation . ^^^ These results suggest that the UV photochemistry of bacterial spore DNA in vivo is due to the binding of alpha / beta type SASP , a binding that is known to cause a change in DNA conformation in vitro from the B form to the A form . ^^^ It is suggested that as these factors diffuse out in the first minutes of spore germination , spore photoproduct yields become similar to those observed for irradiation of SASP / DNA complexes in vitro . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The amino acid sequence predicted from the DNA sequence was identical to that determined previously for SASP B , with the exception of the amino terminal methionine predicted from the gene sequence which is presumably removed posttranslationally and an asparagine residue predicted at position 21 which was originally identified as an aspartate residue . ^^^ The SASP B gene itself hybridized strongly to only one band in Southern blots of restriction enzyme digests of B . megaterium DNA , suggesting that the SASP B gene is not a member of a highly conserved multigene family , as is the case for other SASP genes . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The first Bacillus subtilis small , acid soluble spore protein ( SASP ) gene has been cloned by using previously cloned B . megaterium SASP genes as DNA DNA hybridization probes . ^^^ Determination of the DNA sequence of the B . subtilis SASP gene showed that it codes for a 72 residue protein ( termed SASP 1 ) containing a single spore protease cleavage site as well as other sequences conserved in Bacillus megaterium SASPs A , C , C 1 , C 2 , and C 3 . ^^^ The cloned SASP 1 gene hybridizes to three bands other than the SASP 1 gene itself in EcoRI or HindIII digests of B . subtilis DNA . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The results of hybridization of an sspE gene probe to Southern blots of B . subtilis DNA suggested that there is only a single gene coding for the SASP gamma type of protein in B . subtilis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Three EcoRI fragments of Bacillus megaterium DNA hybridized only under nonrestrictive conditions on Southern blots to a probe containing the previously cloned gene for protein C , a small , acid soluble spore protein ( SASP ) from B . megaterium . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Dry Bacillus subtilis spores lacking their two major DNA binding proteins ( small , acid soluble proteins [ SASP ] alpha and beta ) were much more sensitive to dry heat than were wild type spores . ^^^ These data indicate that SASP alpha and beta provide significant protection to spore DNA against the damaging effects of dry heat . ^^^ This DNA damage may be in part depurination , and a purified alpha / beta type SASP gave significant protection against dry heat induced DNA depurination in vitro . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Alpha / beta type small , acid soluble proteins ( SASP ) of dormant spores of Bacillus subtilis bind to DNA and increase its resistance to a variety of damaging agents both in vivo and in vitro . ^^^ SspC ( 5 ) interacted with DNA similarly to SspC ( wt ) in vivo , resorting UV and heat resistance to spores lacking major alpha / beta type SASP to the same extent as SspC ( wt ) . ^^^ These results confirm the importance of the conserved primary sequence of alpha / beta type SASP in the binding of these proteins to spore DNA and alteration of DNA properties and show further that the GRP recognition region in alpha / beta type SASP plays some role in DNA binding . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Dormant spores of Bacillus subtilis which lack the majority of the alpha / beta type small , acid soluble proteins ( SASP ) ( termed alpha beta spores ) that coat the DNA in wild type spores are significantly more sensitive to hydrogen peroxide than are wild type spores . ^^^ These findings strongly suggest that binding of alpha / beta type SASP to DNA provides one increment of spore hydrogen peroxide resistance . ^^^ Indeed , binding of alpha / beta type SASP to DNA in vitro provides strong protection against cleavage of DNA by hydrogen peroxide . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The DNA in dormant spores of Bacillus species is saturated with a group of nonspecific DNA binding proteins , termed alpha / beta type small , acid soluble spore proteins ( SASP ) . ^^^ These data suggest that binding of alpha / beta type SASP to spore DNA in vivo greatly reduces DNA damage caused by heating , increasing spore heat resistance and long term survival . ^^^ A role for alpha / beta type SASP in protecting DNA against depurination ( and thus promoting spore survival ) was further suggested by the demonstration that these proteins reduce the rate of DNA depurination in vitro at least 20 fold . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Inactivation of the Bacillus subtilis sspF gene had no effect on sporulation , spore resistance , or germination in a wild type strain or one lacking DNA protective alpha / beta type small , acid soluble proteins ( SASP ) . ^^^ In vitro analyses showed that SspF is a DNA binding protein and is cleaved by the SASP specific protease ( GPR ) at a site similar to that cleaved in alpha / beta type SASP . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
SASP and its 2 major metabolites , 5 aminosalicylic acid ( ASA ) and sulfapyridine ( SP ) were tested for in vivo induction of micronuclei in mouse bone marrow cells with or without prefolic treatment and for in vivo formation of DNA adducts in rat and mouse liver and urinary bladder . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Deamidation of one specific asparagine residue in an alpha / beta type small , acid soluble spore protein ( SASP ) of Bacillus subtilis took place readily in vitro ( time for 50 % deamidation [ t ( 1 / 2 ) ] , approximately 1 h at 70 degrees C ) , and the deamidated SASP no longer bound to DNA effectively . ^^^ A mutant alpha / beta type SASP in which the reactive asparagine was changed to aspartate also failed to bind to DNA in vitro , and this protein did not restore UV radiation and heat resistance to spores lacking the majority of their alpha / beta type SASP . ^^^ When expressed in Escherichia coli , where it is bound to DNA , the alpha / beta type SASP deamidated with a t ( 1 / 2 ) of 2 to 3 h at 95 degrees C . ^^^ A gamma type SASP of B . subtilis also deamidated readily in vitro ( t ( 1 / 2 ) for one net deamidation , approximately 1 h at 70 degrees C ) , but this protein ( which is not associated with DNA ) deamidated fairly readily in spores ( t ( 1 / 2 ) , approximately 1 h at 95 degrees C ) . ^^^ However , alpha / beta type SASP are even more strongly protected against deamidation in vivo , presumably by their binding to spore DNA . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
These oxidized alpha / beta type SASP no longer bound to DNA effectively , but DNA binding protected alpha / beta type SASP against methionine oxidation by peroxides in vitro . ^^^ Incubation of an oxidized alpha / beta type SASP with peptidyl methionine sulfoxide reductase ( MsrA ) , which can reduce methionine sulfoxide residues back to methionine , restored the alpha / beta type SASP ' s ability to bind to DNA . ^^^ The major mechanism operating for dealing with oxidative damage to alpha / beta type SASP in spores is DNA binding , which protects the protein ' s methionine residues from oxidation both in vitro and in vivo . ^^^ This may be important in vivo since alpha / beta type SASP containing oxidized methionine residues no longer bind DNA well and alpha / beta type SASP DNA binding is essential for long term spore survival . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Small , acid soluble spore proteins ( SASP ) of the alpha / beta type from several Bacillus species were cross linked into homodimers , heterodimers and homooligomers with 1 ethyl 3 ( 3 dimethylaminopropyl ) carbodiimide ( EDC ) in the presence of linear plasmid DNA . ^^^ These data indicate that , although there are likely to be subtle structural differences between different alpha / beta type SASP , the N terminal regions of these proteins are involved in protein protein interactions while in the DNA bound state . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
These results indicate that : ( 1 ) formaldehyde kills B . subtilis spores at least in part by DNA damage and ( b ) alpha / beta type SASP protect against spore killing by formaldehyde , presumably by protecting spore DNA . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In developing forespores , HBsu colocalized with alpha / beta type SASP on the nucleoid , suggesting that HBsu could modulate alpha / beta type SASP mediated properties of spore DNA . ^^^ Indeed , in vitro studies showed that HBsu altered alpha / beta type SASP protection of pUC 19 from DNase digestion , induced negative DNA supercoiling opposing alpha / beta type SASP mediated positive supercoiling , and greatly ameliorated the alpha / beta type SASP mediated increase in DNA persistence length . ^^^ However , HBsu did not significantly interfere with the alpha / beta type SASP mediated changes in the UV photochemistry of DNA that explain the heightened resistance of spores to UV radiation . ^^^ These data strongly support a role for HBsu in modulating the effects of alpha / beta type SASP on the properties of DNA in the developing and dormant spore . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The DNA in the core of spores of Bacillus species is saturated with a group of small , acid soluble proteins ( SASP ) that protect DNA from a variety of harsh treatments and play a major role in spore resistance and long term spore survival . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Binding of alpha / beta type small acid soluble spore proteins ( SASP ) is the major determinant of DNA resistance to damage caused by UV radiation , heat , and oxidizing agents in spores of Bacillus and Clostridium species . ^^^ Analysis of several alpha / beta type SASP showed that these proteins have essentially no secondary structure in the absence of DNA , but become significantly alpha helical upon binding to double stranded DNAs or oligonucleotides . ^^^ Folding of alpha / beta type SASP induced by a variety of DNAs and oligonucleotides was measured by CD spectroscopy , and this allowed determination of a DNA binding site size of 4 base pairs as well as equilibrium binding parameters of the alpha / beta type SASP DNA interaction . ^^^ Analysis of the equilibrium binding data further allowed determination of both intrinsic binding constants ( K ) and cooperativity factors ( omega ) , as the alpha / beta type SASP DNA interaction was significantly cooperative , with the degree of cooperativity depending on both the bound DNA and the salt concentration . ^^^ Kinetic analysis of the interaction of one alpha / beta type SASP , SspC ( Tyr ) , with DNA indicated that each binding event involves the dimerization of SspC ( Tyr ) monomers at a DNA binding site . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Treatment of wild type spores of Bacillus subtilis with glutaraldehyde or an iodine based disinfectant ( Betadine ) did not cause detectable mutagenesis , and spores ( termed alpha beta ) lacking the major DNA protective alpha / beta type , small , acid soluble proteins ( SASP ) exhibited similar sensitivity to these agents . ^^^ These results indicate that : ( a ) nitrous acid kills B . subtilis spores at least in part by DNA damage , and alpha / beta type SASP protect against this DNA damage ; ( b ) killing of spores by glutaraldehyde or Betadine is not due to DNA damage ; and ( c ) the spore coat protects spores against killing by glutaraldehyde but not Betadine . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The major spore DNA binding proteins , the alpha / beta type small , acid soluble proteins ( SASP ) , colocalized to these nucleoid rings early in spore germination , as did the B . megaterium homolog of the major B . subtilis chromosomal protein HBsu . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The binding of alpha / beta type small , acid soluble spore proteins ( SASP ) to DNA of spores of Bacillus species is the major determinant of DNA resistance to a variety of damaging treatments . ^^^ To determine the functional importance of residues in the N terminal region of alpha / beta type SASP , variants of SspC ( a minor alpha / beta type SASP from Bacillus subtilis ) with modified N termini were generated and their structural and DNA binding properties studied in vitro and in vivo . ^^^ These results are interpreted in the context of a model for DNA dependent alpha / beta type SASP protein protein interaction involving the N terminal regions of these proteins . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Sporulation of a Bacillus subtilis strain ( termed alpha ( ) beta ( ) ) lacking the majority of the alpha / beta type small , acid soluble spore proteins ( SASP ) that are synthesized in the developing forespore and saturate spore DNA exhibited a number of differences from that of the wild type strain , including delayed forespore accumulation of dipicolinic acid , overexpression of forespore specific genes , and delayed expression of at least one mother cell specific gene turned on late in sporulation , although genes turned on earlier in the mother cell were expressed normally in alpha ( ) beta ( ) strains . ^^^ The sporulation defects in alpha ( ) beta ( ) strains were corrected by synthesis of chromosome saturating levels of either of two wild type , alpha / beta type SASP but not by a mutant SASP that binds DNA poorly . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A derivative of SspC , a minor alpha / beta type , small , acid soluble spore protein ( SASP ) from Bacillus subtilis , was generated that has a very high affinity for DNA . ^^^ The outgrowth phenotype of spores with SspC ( Delta 11 D13K ) is proposed to be due to the high stability of the SspC ( Delta 11 D13K ) DNA complex , which prevents rapid degradation of this alpha / beta type SASP early in germination . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The novel UV photochemistry of spore DNA is largely due to its saturation with a group of small , acid soluble proteins ( SASP ) , which are unique to spores and whose binding alters the DNA conformation and thus its photochemistry . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Previous work has shown that spores of wild type Bacillus subtilis are more resistant to killing by dry and wet heat , low vacuum lyophilization and hydrogen peroxide than are spores lacking the majority of their DNA protective alpha / beta type small , acid soluble spore proteins ( SASP ) ( termed alpha ( ) beta ( ) spores ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) The expression of IL 1beta mRNA and IL 8 mRNA was increased significantly in patients with UC , as compared with that in the control specimens ( P < 0 . 05 ) and had a significant positive correlation with NF kappaB DNA binding activity ( r = 0 . 8363 , P < 0 . 05 ; r = 0 . 6024 , P < 0 . 05 , respectively ) . ( 2 ) Glucocorticoids and SASP strongly inhibited NF kappaB activation and signficantly decreased the expression of IL 1beta mRNA and IL 8 mRNA . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The biochemical properties , structure , function , and mechanism of catalysis of the novel spore germination protease ( GPR ) that degrades small , acid soluble proteins ( SASP ) protecting spore ' s DNA against damage and a novel , cofactor independent phosphoglycerate mutase ( iPGM ) are described . ^^^ A coat created from SASP synthesized during sporulation protects DNA in spores and later in the germination this protective coat is removal by a novel GPR protease . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Spores of Bacillus subtilis contain high levels of proteins , termed alpha / beta type small , acid soluble proteins ( SASP ) , that protect the spore ' s DNA against different types of DNA damage . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In this study , wild type spores , mutant spores lacking alpha / beta type small , acid soluble spore proteins ( SASP ) , naked DNA , and complexes between SASP SspC and DNA were exposed simultaneously to UV ( 254 nm ) at intermediate pressure ( 1 2 Pa ) , and the UV photoproducts cis , syn thymine thymine cyclobutane dimer ( c , sTT ) , trans , syn thymine thymine cyclobutane dimer ( t , sTT ) , and `` spore photoproduct ' ' ( SP ) were quantified . ^^^ The changes observed in spore DNA photochemistry at 1 2 Pa in vivo were not reproduced by irradiation of naked DNA or SspC : DNA complexes in vitro , suggesting that factors other than SASP are involved in spore DNA photochemistry at low pressure . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Variants of the wild type Bacillus subtilis alpha / beta type small , acid soluble spore protein ( SASP ) SspC ( wt ) were designed to evaluate the contribution of C terminal residues to these proteins ' affinity for DNA . ^^^ The latter results indicate that only moderately strong binding of alpha / beta type SASP to DNA is important to balance the potentially conflicting requirements for these proteins in DNA transcription and DNA protection during spore formation , spore dormancy , and spore germination and outgrowth . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We previously demonstrated that , in human intrauterine tissues , lipopolysaccharide ( LPS ) stimulated nuclear factor kappaB ( NF kappaB ) DNA binding activity , and subsequent cytokine release can be suppressed by sulfasalazine ( SASP ) concentrations greater than 5 mM . ^^^ In addition , SASP concentrations of 5 mM or greater significantly suppressed NF kappaB DNA binding activity . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A family of highly conserved spore specific DNA binding proteins , termed alpha / beta type small , acid soluble spore proteins ( SASP ) , plays a major role in mediating spore resistance . ^^^ The mechanism by which these proteins exert their protective activity remains poorly understood , in part due to the lack of structural data on the DNA SASP complex . ^^^ By using cryoelectron microscopy , we have determined the structure of the helical complex formed between DNA and SspC , a characteristic member of the alpha / beta type SASP family . ^^^ By effectively sequestering DNA molecules , this dense assembly of filaments is proposed to enhance and complement DNA protection obtained by DNA saturation with the alpha / beta type SASP . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The novel photochemistry of spore DNA is accounted for in part by its reduced hydration , but largely by the saturation of spore DNA with alpha / beta type small , acid soluble spore proteins ( SASP ) . ^^^ The binding of an alpha / beta type SASP to isolated DNA either in dry films or in aqueous solution led to a large decrease in the yield of CPD and 6 4PP , and a concomitant increase in the yield of SP , although levels of interstrand photoproducts were extremely low . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Dehydration and more importantly complexation of DNA by alpha / beta type small , acid soluble spore proteins ( SASP ) have been shown to partly explain the photochemistry of spore DNA . ^^^ We further conclude that the combined effects of alpha / beta type SASP and DPA explain the novel photochemistry of DNA in spores of Bacillus species . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The lack of ExoA and Nfo sensitized spores ( termed alpha beta ) lacking the majority of their DNA protective alpha / beta type small , acid soluble spore proteins ( SASP ) to wet heat . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DPA replete and DPA less FB 122 spores had similar levels of the DNA protective alpha / beta type small acid soluble spore proteins ( SASP ) , but the DPA less spores lacked SASP gamma . ^^^ The inability to synthesize both DPA and most alpha / beta type SASP in strain PS 3664 ( sspA sspB sleB spoVF ) resulted in spores that lost viability during sporulation , at least in part due to DNA damage . ^^^ These and previous results indicate that , in addition to alpha / beta type SASP , DPA also is extremely important in spore resistance and stability and , further , that DPA has some specific role ( s ) in protecting spore DNA from damage . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
However , in the presence of Escherichia coli thioredoxin , the host specified subunit of T 7 DNA polymerase , duplex DNA is hydrolyzed in a 3 ' leads to 5 ' direction to yield nucleoside 5 ' monophosphates . ^^^ The apparent Km for thioredoxin in the reaction is 4 . 8 10 10 ( 8 ) M , a value similar to that for the apparent Km of thioredoxin in the complementation assay with gene 5 protein to restore T 7 DNA polymerase activity . ^^^ Deoxyribonucleoside 5 ' triphosphates inhibit hydrolysis by both exonuclease activities ; hydrolysis of single stranded DNA by the gene 5 protein is inhibited even in the absence of thioredoxin where there is less than 2 % active T 7 DNA polymerase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
This finding suggests that in the T 4 infected cell most of the deoxyribonucleotides required for the viral DNA might be synthesized by the T 4 induced ribonucleotide reductase while the host ribonucleotide reductase is inactive due to the shortage of reduced E . coli thioredoxin . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA polymerase of bacteriophage T 7 is composed of two subunits , the gene 5 protein of the phage and the host specified thioredoxin . ^^^ The purification procedure has been monitored by using a complementation assay in which thioredoxin interacts with the gene 5 protein to form an active DNA polymerase . ^^^ However , T 7 DNA polymerase can be reconstituted by the addition of homogeneous thioredoxin to the gene 5 protein . ^^^ The apparent Km for thioredoxin in the reaction to restore DNA polymerase activity is 2 . 8 10 10 ( 8 ) M . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin forms a molecular complex with the T 7 gene 5 protein , and addition of the host protein restores restores DNA polymerase and double strand exonuclease activities to near normal levels . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Escherichia coli thioredoxin : a subunit of bacteriophage T 7 DNA polymerase . ^^^ The TsnC protein is identical to thioredoxin of E . coli by the following criteria : ( 1 ) Homogeneous preparations of both proteins have TsnC and thioredoxin activity . ( 2 ) Both proteins show similar stability to heat . ( 3 ) They have identical mobilities , corresponding to a molecular weight of 12 , 000 , on polyacrylamide gels containing sodium dodecyl sulfate . ( 4 ) Their amino acid compositions are indistinguishabe . ( 5 ) Antibody prepared against thioredoxin inhibits TsnC activity . ( 6 ) TsnC protein isolated from purified T 7 DNA polymerase has thioredoxin activity . ^^^ In addition , preparations of T 7 DNA polymerase itself exhibit thioredoxin activity and are partially inhibited by antibody to thioredoxin . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Genetic analysis of the interaction between bacteriophage T 7 DNA polymerase and Escherichia coli thioredoxin . ^^^ During infection of Escherichia coli , T 7 annexes the host protein thioredoxin for use as a processivity factor for T 7 DNA polymerase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Analysis of the growth requirements of Epstein Barr virus ( EBV ) transformed B lymphocytes shows that interleukin 1 and thioredoxin , a disulfide reducing enzyme , are able to induce a marked increase in DNA synthesis in the early phases of in vitro culture . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin regulates the DNA binding activity of NF kappa B by reduction of a disulphide bond involving cysteine 62 . ^^^ DNA binding activity of the wild type protein but not the amino acid 62 mutant was also stimulated by thioredoxin while detection of disulphide cross linked dimers in p 50 but not the amino acid 62 mutant suggests that thioredoxin stimulates DNA binding by reduction of a disulphide bond involving cysteine 62 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In contrast to yeast thioredoxin , Southern blot analysis of total yeast genomic DNA indicated that there was only one copy of gene encoding yeast thiotransferase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The activity of ribonucleotide reductase , which catalyzes the first unique step of DNA synthesis and which obtains its reducing equivalents from TR through thioredoxin , was decreased in diaziquone and doxorubicin treated A 204 cells . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Bacteriophage T 7 gene 2 . 5 protein has been shown to interact with T 7 DNA polymerase ( the complex of T 7 gene 5 protein and Escherichia coli thioredoxin ) by affinity chromatography and fluorescence emission anisotropy . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
T 7 DNA polymerase , including thioredoxin and with an active 3 ' 5 ' exonuclease , is unable to bypass aminofluorene adducts , whereas Sequenase 2 bypasses the lesions readily . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA synthesis ) , whereby responding patients ( n = 3 ) appeared to favor the thioredoxin reductase / thioredoxin electron transfer to ribonucleotide reductase , whereas non responders ( n = 4 ) expressed the alternate glutathione reductase / glutaredoxin mechanism . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Rapid isolation of homogeneous cloned T 7 gene 5 protein and T 7 DNA polymerase by affinity chromatography on immobilized thioredoxin . ^^^ Phage T 7 DNA polymerase consists of a strong 1 : 1 complex of T 7 gene 5 protein ( 80 kDa ) and the reduced form of Escherichia coli thioredoxin ( 12 kDa ) . ^^^ The gene 5 protein preparation had an inherent low DNA polymerase and double stranded 3 ' exonuclease activity , which was stimulated at least 30 fold by the presence of reduced thioredoxin . ^^^ Highly active and pure T 7 DNA polymerase was obtained by reconstitution of gene 5 protein with thioredoxin and was isolated by phosphocellulose or FPLC Mono Q chromatography . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The thioredoxin reductase / thioredoxin and the glutathione reductase / glutathione / glutaredoxin electron transfer pathways represent alternative electron donors for ribonucleotide reductase and regulate the synthesis of deoxyribonucleotides , the substrates for DNA synthesis , in the S phase of the cell cycle . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Furthermore , the thioredoxin reductase / thioredoxin system is shown to be a principal electron donor for the ribonucleotide reductases which regulates DNA synthesis . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The amino acid sequence derived from the DNA sequence of the R . sphaeroides gene was identical to the known amino acid sequence of R . sphaeroides thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin reductase ( TR ) is a widely distributed flavoenzyme that provides reduced thioredoxin , a dithiol hydrogen donor for protein disulfide reduction and for the reduction of ribonucleotides to deoxyribonucleotides , the first unique step of DNA synthesis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
RNA DNA hybridization analysis of several specific mRNAs such as trxA ( thioredoxin ) , ssb ( single stranded DNA binding protein ) , uvrD ( DNA helicase 2 ) , cat ( chloramphenicol acetyltransferase ) , nusA ( N utilization substance ) , and pnp ( polynucleotide phosphorylase ) demonstrated two to fourfold increases in their chemical half lives . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Two sequences with homology to a thioredoxin oligonucleotide probe were detected by Southern blot analysis of Anabaena sp . strain PCC 7120 genomic DNA . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Ribonucleotide reductase provides growing cells with deoxyribonucleotides , necessary for DNA synthesis , and thioredoxin is an in vitro hydrogen donor . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin reductase dependent insulin disulfide reduction by phage T 7 DNA polymerase reflects dissociation of the enzyme into subunits . ^^^ Phage T 7 DNA polymerase contains Escherichia coli thioredoxin as a subunit and is a 1 : 1 complex with T 7 gene 5 protein . ^^^ The enzyme showed high thioredoxin activity in assays at 37 degrees C using reduction of insulin disulfides with NADPH and thioredoxin reductase , leading Randahl ( Randahl , H . ( 1982 ) FEBS Lett . 150 , 109 113 ) to propose that the thioredoxin dithiol active site is exposed in T 7 DNA polymerase . ^^^ However , T 7 DNA polymerase and free thioredoxin differ in reactivity with iodoacetic acid after preincubation with dithiothreitol or incubation with insulin . ^^^ The time and temperature dependence of the thioredoxin activity of T 7 DNA polymerase demonstrated that dissociation into subunits at 25 or 37 degrees C accounts for the previously observed activity . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A tight complex of the T 7 gene 5 protein and Escherichia coli thioredoxin provides DNA polymerase activity . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Phage T 7 DNA polymerase consists of a 1 : 1 complex of the viral T 7 gene 5 protein and the host cell thioredoxin . ^^^ After in vitro reconstitution of holoenzyme by addition of excess thioredoxin , highly active T 7 DNA polymerase was purified to homogeneity by a simple antithioredoxin immunoadsorbent chromatography technique . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The nucleotide sequence of a 620 base pair HinfI fragment encoding thioredoxin derived from either plasmid indicated that the protein derived from this DNA is different from the thioredoxin of C . nephridii previously reported ( Meng , M . , and Hogenkamp , H . ^^^ The molecular weight of this thioredoxin , determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis , is smaller than that estimated from the DNA sequence , suggesting that processing may have occurred . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA restriction fragments containing the Anabaena thioredoxin gene were identified by heterologous hybridization to the E . coli thioredoxin gene following Southern transfer , ligated with pUC 13 , and used to transform an E . coli strain lacking functional thioredoxin . ^^^ The amino acid sequence derived from the DNA sequence of the Anabaena gene was identical to the known amino acid sequence of Anabaena thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Deoxyribonucleotides , the precursors of DNA , are formed de novo by ribonucleotide reductase , and in vitro thioredoxin or glutathione plus glutaredoxin have been isolated as hydrogen donors . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The DNA sequence of the Escherichia coli gene encoding thioredoxin reductase has been determined . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T 7 . ^^^ Thioredoxin acts as an accessory protein to bestow processivity on the polymerizing reaction ; DNA synthesis catalyzed by the gene 5 protein thioredoxin complex on a single stranded DNA template can polymerize thousands of nucleotides without dissociation . ^^^ E . coli single stranded DNA binding protein stimulates both the rate of elongation and the processivity of the gene 5 protein thioredoxin complex . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Escherichia coli thioredoxin stabilizes complexes of bacteriophage T 7 DNA polymerase and primed templates . ^^^ The DNA polymerase activity induced after bacteriophage T 7 infection of Escherichia coli is found in a complex of two proteins , the T 7 gene 5 protein and a host protein , thioredoxin . ^^^ The affinity of gene 5 protein for single stranded DNA is at least 1000 fold higher than for double stranded DNA and is little affected by thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The redox capacity of thioredoxin is not required for stimulation of DNA polymerase activity . ^^^ DNA polymerase activity in Escherichia coli cells infected with bacteriophage T 7 resides in a protein complex consisting of the T 7 gene 5 protein and E . coli thioredoxin in a 1 to 1 stoichiometry . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA synthesis is catalyzed by gene 5 protein of the phage , and processivity of DNA synthesis is conferred by Escherichia coli thioredoxin , a protein that is tightly associated with gene 5 protein . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The heat stable cytosolic factor that promotes glucocorticoid receptor binding to DNA is neither thioredoxin nor ribonuclease . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Here we describe the sequence of cloned rat liver PDI complementary DNA which predicts a protein with two distinct regions homologous with Escherichia coli thioredoxin , a known cofactor in oxidation reduction reactions . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin serves as a cofactor for reductive processes in many cell types and is a constituent of phage T 7 DNA polymerase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Bacteriophage T 7 induced DNA polymerase is composed of a 1 : 1 complex of phage induced gene 5 protein and Escherichia coli thioredoxin . ^^^ Thioredoxin sulfhydryl groups present in native T 7 DNA polymerase do not appear to be involved in an intersubunit disulfide bond ; one and probably both sulfhydryls are available in the native holoenzyme for modification by N ethylmaleimide . ^^^ Furthermore , DNA substrates alter the reactivity of thioredoxin cysteines within the holoenzyme with respect to this reagent . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
These results suggest a role for thioredoxin in plasmid DNA replication . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Phage T 7 DNA polymerase purified to homogeneity by an antithioredoxin immunoadsorbent technique was resolved into its active subunits the gene 5 protein and Escherichia coli thioredoxin by a novel technique involving chromatography on Sephadex G 50 at pH 11 . 5 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The immunoadsorbent used to purify T 7 DNA polymerase contains antibodies directed towards thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
T 7 DNA polymerase reduced insulin at the same Km as thioredoxin , while the turnover number decreased . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The bacteriophage T 7 induced DNA polymerase , consisting of the phage specified gene 5 protein associated with Escherichia coli thioredoxin , catalyzes the copolymerization of SP dATP alpha S with dTTP , producing the alternating of polymer poly [ dTs A ) ] by a mechanism involving inversion of configuration at P alpha . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The gene encoding thioredoxin ( trxA ) was isolated from chromosomal DNA of E . coli HB 101 strain using the polymerase chain reaction . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The DNA sequence encoding human placental thioredoxin reductase has been determined . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A redox dependent function of thioredoxin is necessary to sustain a rapid rate of DNA synthesis in yeast . ^^^ DNA replication is impaired in mutants of Saccharomyces cerevisiae which lack the two thioredoxin genes TRX 1 and TRX 2 . ^^^ The results show that an optimal rate of DNA synthesis requires a redox function of thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Deoxyribonucleotides are maintained at normal levels in a yeast thioredoxin mutant defective in DNA synthesis . ^^^ Deletion of both thioredoxin genes TRX 1 and TRX 2 of Saccharomyces cerevisiae reduces the rate of DNA replication . ^^^ Since thioredoxin is a hydrogen donor to ribonucleotide reductase , a priori the inhibition of DNA synthesis was predicted to be caused by a reduction in the deoxyribonucleotide pools . ^^^ The second proposes that thioredoxin regulates the tempo of DNA replication directly by activating a component of the replication machinery . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We have examined the effect of thioredoxin , an accessory protein that confers high processivity to bacteriophage T 7 DNA polymerase , on the fidelity of DNA synthesis . ^^^ In the presence of thioredoxin , exonuclease proficient T 7 DNA polymerase is highly accurate . ^^^ In the absence of thioredoxin , T 7 DNA polymerase is 3 fold more accurate for base substitutions and > or = 27 fold and 9 fold more accurate , respectively , for 1 and 2 nt deletion errors at nonreiterated nucleotide sequences . ^^^ In marked contrast , the rate for frameshift errors wherein one or more nucleotides has been added to a repeated DNA sequence increases 46 fold when thioredoxin is absent from the polymerization reaction . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A well characterized B chronic lymphocytic leukaemia ( B CLL ) clone ( 1 83 ) was used as a source of monoclonal B cells inducible to DNA synthesis and differentiation by using 12 O tetradecanoyl phorbol 13 acetate ( TPA ) in combination with interleukin 4 ( IL 4 ) and thioredoxin ( Trx ) containing supernatant from a T cell hybridoma ( BSF MP 6 ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Recombinant human thioredoxin stimulated DNA synthesis and the proliferation of murine fibroblasts . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In vitro , GSSG inhibited the DNA binding activity of NF kappa B more effectively than that of AP 1 , while AP 1 was inhibited more effectively by oxidized thioredoxin . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The results demonstrate that the solution behavior of the mutant protein is similar in many ways to that of reduced wild type thioredoxin , explaining its partial activity in the two essential roles of reduced thioredoxin as a subunit of phage T 7 DNA polymerase and in the assembly of filamentous phage . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The genes that encode thioredoxin and thioredoxin reductase of Streptomyces clavuligerus were cloned , and their DNA sequences were determined . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Spinach thioredoxin m inhibits DNA synthesis in fertilized Xenopus eggs . ^^^ A role for thioredoxin in metazoan DNA synthesis has been assessed by injecting rapidly dividing Xenopus eggs with purified heterologous thioredoxins , which might act as inhibitors if they were to replace resident thioredoxins in some but not all reaction steps . ^^^ In egg extracts , thioredoxin m inhibits incorporation of radioactive dCTP into DNA of sperm nuclei and M 13 phage . ^^^ Inhibition exceeds 90 % when thioredoxin m and M 13 DNA are preincubated together . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA binding and transactivation by AP 1 were induced in HeLa cells upon treatment with the antioxidants pyrrolidine dithiocarbamate ( PDTC ) and N acetyl L cysteine ( NAC ) , and upon transient expression of the antioxidative enzyme thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We show that a cellular reducing catalyst thioredoxin ( Trx ) plays a major role in activation of the DNA binding of NF kappa B in vitro and stimulation of transcription from the NF kappa B dependent gene expression . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The direct redox regulation of DNA binding has not been described in vivo , possibly because most protein thiol groups are strongly buffered against oxidation by the highly reduced intracellular environment mediated by glutathione , thioredoxin , and associated pathways . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
C . nephridii thioredoxin C 3 is able to serve as a subunit of T 7 DNA polymerase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The following can be concluded ( a ) glycine 74 of R . sphaeroides thioredoxin has a direct role in the binding of T 7 gene 5 protein and the hydrophobic area of thioredoxin ; ( b ) the N terminus plays a role in maintaining the conformational integrity of the active site ; ( c ) the flexibility of Gly 74 in the hydrophobic region involved in protein / protein interaction is the operative factor in the case of the activity of thioredoxin in the T 7 DNA polymerase . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Amino acid residues critical for the interaction between bacteriophage T 7 DNA polymerase and Escherichia coli thioredoxin . ^^^ Upon infection of Escherichia coli , bacteriophage T 7 annexes a host protein , thioredoxin , to serve as a processivity factor for its DNA polymerase , T 7 gene 5 protein . ^^^ The suppressor E319K mutation , positioned within the DNA polymerization domain of gene 5 protein , appears to suppress the parental thioredoxin mutation by compensating for the binding defect that was caused by the G74D alteration . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The solution structure of human thioredoxin complexed with its target from Ref 1 reveals peptide chain reversal . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin stimulates the DNA polymerase activity of the C terminal fragments . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The results are discussed in light of remarkably similar observations with T 7 DNA polymerase in the presence or absence of thioredoxin , an accessory subunit that affects these same properties . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Twenty one of the 105 major ORFs resembled proteins in databases including ribonucleotide reductase small subunit , RNase 3 , thioredoxin , glutaredoxin , protein disulfide isomerase , deoxynucleoside kinase , frog virus 3 ATPase , Acetobacter cellulose synthase , a bacteriophage encoded endonuclease , and two C 5 cytosine DNA methyltransferases . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA binding of recombinant GABP alpha was activated by chemical reduction ( dithiothreitol ) and by thioredoxin ; however , GSSG inhibited GABP DNA binding activity . ^^^ Our results suggest that GABP DNA binding activity is redox regulated in vivo , possibly by thioredoxin mediated reduction and by GSSG mediated oxidation of the GABP alpha subunit . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Cells treated with 1 chloro 2 , 4 dinitrobenzene , an irreversible inhibitor of thioredoxin reductase , exhibited reduced endogenous NF Y DNA binding activity . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Furthermore , the addition of reduced thioredoxin to cell extracts enhanced HIF 1 DNA binding . ^^^ Consistent with these results , overexpression of thioredoxin and Ref 1 significantly potentiated hypoxia induced expression of a reporter construct containing the wild type HIF 1 binding site . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits , a thioredoxin , a xylanase and an amino acid transporter . ^^^ These included a pair of newly identified DNA gyrase subunits A ( grlA ) and B ( grlB ) , a sodium / proton dependent alanine carrier ( alsT ) , a member of the thioredoxin family ( TlpA ) , an endo 1 , 4 beta xylanase ( xynD ) and a response regulator protein . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
To circumvent these limitations , hTSHR complementary DNA encoding the extracellular domain ( hTSHR ecd ; amino acids 21 415 ) was inserted into the vector pGEX 2TK by directional cloning and used to transform the thioredoxin reductase mutant strain of E . coli ( Ad 494 ) , which allowed formation of disulfide bonds in the cytoplasm . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The thioredoxin binding domain of bacteriophage T 7 DNA polymerase confers processivity on Escherichia coli DNA polymerase 1 . ^^^ The high processivity of T 7 DNA polymerase is achieved through tight binding to its processivity factor , E . coli thioredoxin . ^^^ Insertion of this domain into the homologous site in E . coli DNA polymerase 1 results in a dramatic increase in the processivity of the chimeric DNA polymerase , a phenomenon that is dependent upon its binding to thioredoxin . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
T 7 gene 5 DNA polymerase forms a complex with Escherichia coli thioredoxin ( its processivity factor ) , and a 76 amino acid sequence ( residues 258 334 ) , unique to gene 5 protein , has been implicated in this interaction . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The function of the Drosophila thioredoxin homologue encoded by the deadhead gene is redox dependent and blocks the initiation of development but not DNA synthesis . ^^^ A number of studies have documented that thioredoxin plays a role in DNA synthesis . ^^^ If thioredoxin is required for DNA synthesis in the fly , then deadhead mutations will suppress mutations that inappropriately synthesize DNA . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The in vitro system exhibits the same component requirements seen in vivo : functional thioredoxin , an intact packaging signal in the substrate DNA , and the assembly protein , pIV . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
To overcome problems with site directed mutagenesis and low expression , the thioredoxin reductase gene was adapted for use in the plasmid vector pSL 350 ( Brosius , J . , Methods Enzymol . 216 , 469 483 , 1992 ) , which is designed both for protein expression and for production of single stranded template DNA for mutagenesis , and examined expression of wild type thioredoxin reductase under different growth conditions . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We have also found that two cellular thiol reactive proteins , thioredoxin and Ref 1 , work effectively and synergistically for activation of the Runt domain . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Interleukin 15 + thioredoxin induce DNA synthesis in B chronic lymphocytic leukemia cells but not in normal B cells . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin ( Trx ) is a small ubiquitous dithiol protein which together with the FAD containing enzyme thioredoxin reductase ( TR ) and NADPH ( the Trx system ) is a hydrogen donor for ribonucleotide reductase essential for DNA synthesis and a general protein disulfide reductase involved in redox regulation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The ability to sensitize E . coli to the lethal and mutagenic effects of DBE and DBM seems restricted to DNA alkyltransferase , since overexpression of thioredoxin ( Trx ) or glutaredoxin ( Grx 1 ) in ogt ada cells showed no effect , in spite of the reported potential of bioactive dihaloethane derived species to alkylate Trx . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The redox protein thioredoxin plays an important role in controlling cancer cell growth through regulation of DNA synthesis and transcription factor activity . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin from Streptomyces aureofaciens controls coiling of plasmid DNA . ^^^ A number of potential functions of thioredoxin have been proposed in literature , including a role for DNA replication . ^^^ The aim of our study was to investigate the effects of thioredoxin from Streptomyces aureofaciens ( Trx S . a . ) on plasmid DNA . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The classic function for thioredoxin is to act as a hydrogen donor for the enzyme ribonucleotide reductase , which is essential for DNA synthesis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Overexpression of thioredoxin in Fanconi anemia fibroblasts prevents the cytotoxic and DNA damaging effect of mitomycin C and diepoxybutane . ^^^ We observed that overexpression of the thioredoxin cDNA in FA fibroblasts completely abolished the DNA damaging effects of mitomycin C and diepoxybutane and inhibited the constitutive activity of the nuclear factor kappaB ( NF kappaB ) in SV 40 transformed FA fibroblasts . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
As is true for other members of this peroxiredoxin family , the nDiTPx encoded protein had the conserved cysteine near the amino terminus , considered to be essential for enzyme activity . nDiTPx was expressed in E . coli and the resulting recombinant fusion protein was shown to have thioredoxin peroxidase ( TPx ) activity , by its ability to protect DNA from oxidative nicking in a metal catalyzed oxidation system . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Likewise T 7 DNA polymerase , composed of the T 7 gene 5 protein and Escherichia coli thioredoxin , was blocked by all the photoproducts , but the exo mutant Sequenase 2 . 0 was able to bypass them all in the order cis syn > Dewar > trans syn 2 > ( 6 4 ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We report here that AP 2 DNA binding in vitro can be reversibly modulated by redox conditions and that thioredoxin is a potent stimulator of AP 2 DNA binding . ^^^ The inhibitory effect of diamide on AP 2 DNA binding was dose dependent and was reversible by addition of reducing agents beta mercaptoethanol , dithiothreitol , and thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Previously , we showed that the trxA and trxB genes , coding for thioredoxin and thioredoxin reductase , respectively , occur in S . coelicolor as a gene cluster and are contained on a cosmid H 24 that carries oriC and several genes involved in DNA replication . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
This phage induced alignment technique has been used to study 1H 1H , 1H 13C , and 1H 15N dipolar coupling interactions in a DNA duplex , an RNA hairpin and several proteins including thioredoxin and apo calmodulin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
However , additional putative genes not expected on an extrachromosomal element , such as those encoding an electron transport chain cytochrome d oxidase , DNA nucleotide synthesis enzymes thioredoxin and thioredoxin reductase , and eukaryotic like TATA binding protein transcription factors and a chromosomal replication initiator protein were also found . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The DNA sequence encoding a novel human thioredoxin reductase has been determined . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Reaction between an intein generated C terminal thioester on E . coli maltose binding protein ( 43 kDa ) and an intein generated cysteine at the N terminus of either T 4 DNA ligase ( 56 kDa ) or thioredoxin ( 12 kDa ) resulted in the ligation of the proteins through a native peptide bond . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Co transfection experiments revealed that expression of Ref 1 and thioredoxin further enhanced the luciferase activity expressed by CAD , but not by NAD . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thus , even when p 53 was tethered to a reporter gene by a heterologous DNA binding domain , reporter gene transactivation remained dependent on thioredoxin reductase . ^^^ Experiments using additional LexA / p53 truncation mutations indicated that the p 53 negative regulatory domain , rather than the DNA binding or oligomerization domains , created the requirement for thioredoxin reductase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Incubation of 100 microM [ 14C ] HMAF ( 24 hr ) with purified DNA , serum albumin , thioredoxin , and thioredoxin reductase resulted in 6 , 22 , 14 , and 11 pmol [ 14C ] HMAF / microg DNA or protein , respectively . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Peptide 83 is able to display dual biological functions of thioredoxin , namely , a substrate for the enzyme E . coli thioredoxin reductase and a processivity factor of T 7 DNA polymerase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Structural similarity is also found with E . coli thioredoxin , the accessory subunit and processivity factor in bacteriophage T 7 DNA polymerase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Cellular redox factor , thioredoxin , was able to replace DTT in stimulation of RPA DNA binding activity , suggesting that redox protein may be involved in RPA modulation in vivo . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
This showed that GRX may be involved in the redox regulation of the DNA binding activity of PEBP 2 as is the case with thioredoxin . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
On the basis of protease protection data , we propose that the preinitiation complex is converted to an initiation complex by binding phage DNA , thioredoxin and the initiating minor coat protein ( s ) . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Reactive oxygen intermediates , thioredoxin , and Ref 1 as effector molecules in cellular signal transduction ] . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Two DNA constructs containing the wheat thioredoxin h gene ( wtrxh ) were used for transformation ; each contained wtrxh fused to an endosperm specific B ( 1 ) hordein promoter either with or without a signal peptide sequence for targeting to the protein body . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin is a small multifunctional protein which acts as a dithiol hydrogen donor for ribonucleotide reductase in DNA synthesis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The expression of drug resistance related genes including glutathione S transferase pi , gamma glutamyl cysteine synthetase , multidrug resistance 1 , multidrug resistance associated protein , DNA topoisomerase 1 , topoisomerase 2 , human canalicular multispecific organic anion transporter , and thioredoxin was not significantly related to cisplatin resistance . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A growing number of transcription factors including NF kappaB or the Ref 1 dependent AP 1 require thioredoxin reduction for DNA binding . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Complex reduction and DNA release can be induced by various thiols as well as enzymatically , by thioredoxin reductase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Mechanisms by which thioredoxin increases cell growth include an increased supply of reducing equivalents for DNA synthesis , activation of transcription factors that regulate cell growth , and an increase in the sensitivity of cells to other cytokines and growth factors . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
To increase the amount of soluble form and activity of recombinant LIP 4 , the DNA was fused to the gene for thioredoxin ( TrxFus LIP 4 ) and then expressed in E . coli strain AD 494 ( DE 3 ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
However , as demonstrated in immuno coprecipitation experiments , the kinetics of NF kappaB DNA binding is strictly paralleled by decreased and increased complex formation between NF kappaB and thioredoxin ( Trx ) , the reducing catalyst of Cys 62 of NF kappaB subunit p 50 , the reduced thiol function of which is essential for efficient NF kappaB DNA binding . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The relationships of oxidative stress with FA phenotype rely on a consistent body of evidence that includes : ( 1 ) excess formation of DNA oxidative damage ( both in vitro and in vivo ) ; ( 2 ) cellular protection by hypoxia , low molecular weight antioxidants , antioxidant enzymes , and thioredoxin overexpression ; ( 3 ) impaired expression and / or activity of antioxidant enzymes , and ( 4 ) the redox dependent action mechanisms of MMC and DEB . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Modulation of exogenous and endogenous levels of thioredoxin in human skin fibroblasts prevents DNA damaging effect of ultraviolet A radiation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Exonucleolytic degradation by the 3 ' > 5 ' exonuclease activity of T 7 DNA polymerase ( wild type ) in the presence of excess reduced thioredoxin proceeded to complete breakdown of the labeled DNAs . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Since glutathione and thioredoxin can protect from drug induced apoptosis , their upregulation in response to respiratory chain generated reactive oxygen might represent a cellular adaptation to DNA damage that promotes cell survival . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A probe based on the sequence of the gene encoding selenoprotein A of glycine reductase of Clostridium sticklandii was used to obtain clones of adjacent DNA that encoded the other components of glycine reductase , proteins B and C , in addition to thioredoxin and thioredoxin reductase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Sodium selenite ( Na Se ) present in glutathione peroxidase and thioredoxin reductase and addition of glutathione ( GSH ) prevented DNA damage . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Sulforaphane could either directly inactivate NF kappa B subunits by binding to essential Cys residues or interact with glutathione or other redox regulators like thioredoxin and Ref 1 relevant for NF kappa B function . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The crystal structure of the DNA polymerase encoded by gene 5 of bacteriophage T 7 , in a complex with its processivity factor , Escherichia coli thioredoxin , a primer template , and an incoming deoxynucleoside triphosphate reveals a putative hydrogen bond between the C terminal residue , histidine 704 of gene 5 protein , and an oxygen atom on the penultimate phosphate diester of the primer strand . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In particular , we stress the possible involvement of thioredoxin , Ref 1 ( redox factor 1 ) , and metallothionein in the control of p 53 protein conformation and activity . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A DNA encoding thioredoxin mature carp ovarian cystatin ( trx cystatin ) fusion protein was ligated into a pET 23a ( + ) expression vector and then transformed into Escherichia coli AD 494 ( DE 3 ) expression host . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We reported previously that thioredoxin ( TRX ) regulates the DNA binding activities of ER and p 53 in vitro . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We compare results using the GB model ( or GB plus a surface area based `` hydrophobic ' ' term ) to explicit solvent simulations for a 10 base pair DNA oligomer , and for the 108 residue protein thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA microarray reveals increased expression of thioredoxin peroxidase in thioredoxin 1 transfected cells and its functional consequences . ^^^ Reduced thioredoxins reduce oxidized cysteine groups on proteins including transcription factors to increase their binding to DNA , and is a source of reducing equivalents for enzymes such as thioredoxin peroxidase which removes H2O2 and alkyl peroxides . ^^^ We have used DNA micro array to investigate the effects of thioredoxin 1 transfection on the expression of a panel of 520 redox , apoptosis and cell growth related genes in MCF 7 human breast cancer cells . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Recombinant fusion proteins , ovalbumin LHRH 7 and thioredoxin LHRH 7 , were generated using recombinant DNA technology and expressed in E . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
It is shown that the Chlamydomonas peroxiredoxin ( Ch Prx 1 ) is active with various thioredoxin isoforms , functions as an antioxidant toward reactive oxygen species ( ROS ) , and protects DNA against ROS induced degradation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Here , we demonstrate , using a proteomic approach , that an H 19 overexpression in human cancerous mammary epithelial cells stably transfected with genomic DNA containing the entire H 19 gene is responsible for positively regulating at the post transcriptional level the thioredoxin , a key protein of the cellular redox metabolism . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
However , detailed analysis of its three dimensional structure along with molecular docking simulations of its interaction with T 7 DNA polymerase ( a thioredoxin specific substrate ) and comparisons with other known members of the thioredoxin / glutaredoxin family of proteins strongly suggest that MtH 895 is more akin to a thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Since thioredoxin is known to be the reactive cofactor of ribonucleotid reductase its shortcoming reduces the supply of deoxyribonucleotides thus hindering the DNA and replication repair with resultant chromosomal breaks . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The genomic DNA encoding thioredoxin ( TRX ) was previously isolated from the fission yeast Schizosaccharomyces pombe . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
During the cloning of a genomic DNA encoding mitochondrial thioredoxin ( TRX ) from the fission yeast Schizosaccharomyces pombe , its 5 ' flanking sequence was involved in the high frequency of transformation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A genomic DNA encoding a second thioredoxin ( TRX 2 ) was isolated from the chromosomal DNA of the fission yeast Schizosaccharomyces pombe . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The DNA encoding chicken lung cystatin was ligated into a thioredoxin pET 23a+ expression vector and transformed into Escherichia coli AD 494 ( DE 3 ) pLysS . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Real time quantitative PCR ( RT PCR ) confirmed the significant changes in 4 of 4 mRNAs assayed ( CYP1A1 , HO 1 , GSR , CDKN 1 ) , and identified 3 additional mRNA changes ; 2 redox related genes , gamma glutamate cysteine ligase modifier subunit ( GCLM ) and thioredoxin reductase ( TXNRD 1 ) and 1 DNA replication gene , topoisomerase IIalpha ( TOP2A ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Short term treatment with 0 . 1 5 microM As ( 3 ) up regulates expression of c Fos and c Jun and the redox regulators , thioredoxin ( Trx ) and Redox factor 1 ( Ref 1 ) and activates both AP 1 and NF kappa B binding . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Since ionizing radiation ( IR ) induces oxidative stress as well as increases AP 1 DNA binding activity via the activation of Ref 1 , the potential roles of TR and thioredoxin ( TRX ) in the regulation of AP 1 activity in response to IR were investigated . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Recently , we found that thioredoxin ( TRX ) , a disulfide reductase enzyme known to be important for the binding of transcription factors to DNA , contributes to the induction of HO 1 by inflammatory mediators . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Sixteen such proteins were present in the supernatant : VacA ; a conserved secreted protein ( HP 1286 ) ; putative peptidyl cis trans isomerase ( HP 0175 ) ; six proteins encoded by HP 0305 , HP 0231 , HP 0973 , HP 0721 , HP 0129 , and HP 0902 ; thioredoxin ( HP 1458 ) ; single stranded DNA binding 12RNP2 precursor ( HP 0827 ) ; histone like DNA binding protein HU ( HP 0835 ) ; ribosomal protein L 11 ( HP 1202 ) ; a putative outer membrane protein ( HP 1564 ) ; and outer membrane proteins Omp 21 ( HP 0913 ) and Omp 20 ( HP 0912 ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA mismatch repair gene mutS ( 2 . 56 kb ) was PCR modified and cloned into a secretive prokaryotic expression vector pET32a ( + ) which carries a N terminal His . tag + and thioredoxin sequence . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
It is demonstrated that thioredoxin 2 of R . capsulatus can partially replace the thioredoxin 1 function as a hydrogen donor for methionine sulfoxide reductase but can not replace thioredoxin 1 as a subunit of phage T 7 DNA polymerase . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Real time kinetics of the interaction between the two subunits , Escherichia coli thioredoxin and gene 5 protein of phage T 7 DNA polymerase . ^^^ T 7 phage DNA polymerase is a tight 1 : 1 complex of the gene 5 protein ( g5p ) ( 80 kDa ) of phage T 7 and thioredoxin ( 12 kDa ) from the Escherichia coli host . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A covalent linkage between the gene 5 DNA polymerase of bacteriophage T 7 and Escherichia coli thioredoxin , the processivity factor : fate of thioredoxin during DNA synthesis . ^^^ Gene 5 protein ( gp 5 ) of bacteriophage T 7 is a non processive DNA polymerase , which acquires high processivity by binding to Escherichia coli thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Specific genome features include deficiencies in amino acid metabolisms , the lack of clear thioredoxin and thioredoxin reductase homologs , and a mutation in DNA gyrase predicting a resistance to quinolone antibiotics . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Insertion of the T 3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase . ^^^ Insertion of the T 3 DNA polymerase thioredoxin binding domain ( TBD ) into the distantly related thermostable Taq DNA polymerase at an analogous position in the thumb domain , converts the Taq DNA polymerase from a low processive to a highly processive enzyme . ^^^ The enhancement in processivity is 20 50 fold when compared with the wild type Taq DNA polymerase or to the recombinant polymerase in the absence of thioredoxin . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
U 09860 ) was synthesized from 26 oligonucleotides by polymerase chain reaction and cloned into plasmid pET 32a downstream to the gene of fusion partner thioredoxin immediately after the DNA sequence encoding enteropeptidase recognition site . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
This study was aimed to determine the effects of cysteamine on redox sensitive Egr 1 transcriptional activity and on other thiol containing proteins such as redox factor 1 ( Ref 1 ) and thioredoxin ( Trx ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin ( Trx ) and glutaredoxin ( Grx ) are dithiol redox enzymes , catalyzing general thiol disulfide oxidoreductions apart from being hydrogen donors for ribonucleotide reductase , an enzyme essential for DNA synthesis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
RESULTS : Expression of VDUP 1 , which was reported as an inhibitor of thioredoxin , was induced rapidly and constantly after exposure to high concentrations of glucose upon analysis with DNA microarray . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Using immunohistochemistry and Western blotting , we investigated the oxidative stress and redox related molecules , such as 8 hydroxy 2 ' deoxyguanosine ( 8 OHdG ) , 4 hydroxynonenal ( 4 HNE ) , thioredoxin ( TRX ) and redox factor 1 ( ref 1 ) in the placenta in preeclampsia , intrauterine growth restriction ( IUGR ) , preeclampsia + IUGR and in normal pregnancy . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The expression of thioredoxin , a marker of oxidative stress overload , as well as 8 hydroxy 2 ' deoxyguanosine , an established marker of DNA damage , in the myocardium was investigated . ^^^ In addition , the up regulation of myocardial thioredoxin with DNA damage , which was induced by the inflammatory stimuli by the virus , was suppressed by the CoQ 10 treatment , which may reflect the anti oxidant effects of CoQ 10 treatment . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin ( Trx ) is a dithiol reducing enzyme known to be involved in the redox regulation of a number of transcription factors , and in this study , we have investigated the redox dependent regulation of the DNA binding activity of Sp 1 by thioredoxin . ^^^ We found that thioredoxin alone or in conjunction with the full thioredoxin system ( comprising thioredoxin , thioredoxin reductase [ TR ] , and alpha nicotinamide adenine dinucleotide phosphate [ NADPH ] ) can increase Sp 1 DNA binding activity in vitro to an oligonucleotide containing the Sp 1 consensus sequence . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA encoding a modified pro peptide from bovine chymosin was fused upstream of hirudin , carp growth hormone , thioredoxin and cystatin coding sequences and expressed in a bacterial Escherichia coli host . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
To overcome these potential problems , we fused the DNA coding sequence of a magainin gene analogue within the sequence of a bacterial thioredoxin gene . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Up regulation of the redox mediators thioredoxin and apurinic / apyrimidinic excision ( APE ) / Ref 1 in hypoxic microregions of invasive cervical carcinomas , mapped using multispectral , wide field fluorescence image analysis . ^^^ Thioredoxin and apurinic / apyrimidinic excision ( APE ) / ref 1 are important redox mediators in biochemical pathways that promote cell survival under adverse conditions including hypoxia and oxidative stress . ^^^ Because elevated thioredoxin or APE / ref 1 is also associated with resistance to certain forms of cancer treatment , we examined their tissue distribution in a series of 110 cervical carcinoma biopsies . ^^^ Analysis was done using a quadruple fluorescence imaging technique , incorporating carbonic anhydrase 9 ( CAIX ) immunofluorescence to outline hypoxic microregions and 4 ' , 6 diamidino 2 phenylindole to localize nuclear staining of thioredoxin and APE / ref 1 . ^^^ Thioredoxin and APE / ref 1 levels were expressed as the average pixel brightness in tumor tissue , subdivided based on CAIX and 4 ' , 6 diamidino 2 phenylindole staining . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The crystal structure of T 7 DNA polymerase or gene 5 protein complexed to its processivity factor , Escherichia coli thioredoxin , a primer template , and a dideoxynucleotide reveals how this enzyme interacts with the 3 ' end of the primer template , but does not show how thioredoxin confers processivity to the polymerase . ^^^ Our data with methylphosphonate modified DNA suggests that thioredoxin confers processivity to T 7 DNA polymerase in part by causing an interaction with the phosphate backbone or minor groove of DNA . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The unique putative gene for thioredoxin reductase ( TrxR ) was isolated from the chromosomal DNA of the fission yeast Schizosaccharomyces pombe . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The reducing enzyme Thioredoxin ( but not Ape 1 ) could restore DNA binding activity of Oct 4 . ^^^ Thioredoxin was less effective at restoring the DNA binding ability of FoxD 3 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Redox cycling of anthracyclines by thioredoxin system : increased superoxide generation and DNA damage . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A crystal structure of the bacteriophage T 7 gene 5 protein / Escherichia coli thioredoxin complex reveals a region in the exonuclease domain ( residues 144 157 ) that is not present in other members of the E . coli DNA polymerase 1 family . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We found that the high levels of NF kappaB DNA binding activity and thioredoxin reductase , together with inhibitory AP 1 complexes , allowed increased expression of antioxidant enzymes and survival of C2C12 cells after oxidant exposure . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Recombinant thioredoxin significantly inhibited DNA synthesis of primary cultured stellate cells under serum or platelet derived growth factor stimulation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
During the microarray and PCR analyses , the metabolism and oxidative stress related genes , such as Cyp1a , P 450 oxidoreductase ( Por ) , and thioredoxin reductase 1 ( Txnrd 1 ) ; a few DNA damage / repair genes , such as 8 oxoguanine DNA glycosylase 1 ( Ogg 1 ) ; and growth arrest and DNA damage inducible 45 alpha ( Gadd45a ) , were fluctuated in this group , together with a slight increase in the concentration of activated NF kappaB . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Compartmentation of Nrf 2 redox control : regulation of cytoplasmic activation by glutathione and DNA binding by thioredoxin 1 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In this in vitro study , we discovered that the AP 1 / Ref 1 / thioredoxin signaling pathway is altered in aortic VSMC from iNOS KO mice , which leads to reduced growth response when compared with aortic VSMC from WT mice . ^^^ In addition , KO cells had less nuclear reducing factor 1 ( Ref 1 ) and serum inducible thioredoxin protein expression . ^^^ Reduced proliferative response of iNOS KO VSMC to serum treatment is associated with altered AP 1 / Ref 1 / thioredoxin pathway activation . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Functional specialization of Chlamydomonas reinhardtii cytosolic thioredoxin h 1 in the response to alkylation induced DNA damage . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In contrast , the DNA binding activity of oxidized NF kappaB is significantly diminished , and that activity is restored by reducing enzymes , such as thioredoxin or redox factor 1 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Bacteriophage T 7 DNA polymerase ( gene 5 protein , gp 5 ) interacts with its processivity factor , Escherichia coli thioredoxin , via a unique loop at the tip of the thumb subdomain . ^^^ We propose that the thioredoxin binding domain is a molecular switch that regulates the interaction of T 7 DNA polymerase with other proteins of the replisome . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin ( TRX ) , which is a stress inducible protein with redox active disulfide structures , has various biological activities by regulating DNA binding of transcription factors in cells . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In this study , AF 17121 was fused to thioredoxin by recombinant DNA techniques and examined for IL5Ralpha interaction using a surface plasmon resonance biosensor method . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA fragmentation was accompanied by the following phenomena : elevation in the level of hemeoxygenase 1 protein and thioredoxin reductase mRNA ; repression of Mn superoxide dismutase and catalase mRNAs ; release of cytochrome c from mitochondria into the cytosol ; activation of caspases 8 , 9 and 3 ; decrease in the level of Bcl 2 , Bcl XL and Bid protein ; increase in the level of Bad protein . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
RESULTS : 18 protein spots were differentially expressed as compared with age matched nondemented control brains which were identified as glyceraldehyde 3 phosphate dehydrogenase , uracil DNA glycosylase , human superoxide dismutase , isocitrate dehydrogenase subunit , synaptotagmin 1 , thioredoxin peroxidase 1 , glial fibrillary acidic protein , p 25 alpha , enoyl coenzyme A hydratase short chain 1 , pyridoxine 5 ' phosphate oxidase , Mn superoxide dismutase and alpha enolase , antioxidant protein 2 , ferritin heavy chain , glutamate dehydrogenase precursor , peptidyl prolyl cis trans isomerase A , serum albumin precursor and dihydropyrimidinase related protein 2 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Using DNA microarray analysis , we found that human macrophages respond to oxidized low density lipoprotein ( oxLDL ) by activating the antioxidative glutathione and thioredoxin systems . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
An HIV 1 encoded peptide mimics the DNA binding loop of NF kappaB and binds thioredoxin with high affinity . ^^^ Pro fs has significant sequence similarity to the DNA binding loop of nuclear factor kappa B ( NF kappaB ) , which is known to bind thioredoxin ( Trx ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
ASK 1 inhibition with thioredoxin attenuated high tidal volume ventilation induced MIP 2 messenger RNA expression , MIP 2 protein production , neutrophil migration into the lung , airway epithelial cell apoptosis , activation of JNK , and AP 1 DNA binding . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The human selenoprotein thioredoxin reductase is involved in antioxidant defense and DNA synthesis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Glutathione S transferase , thioredoxin , glutaredoxins , and DNA repair enzymes responded most strongly to cadmium and chromate . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Time dependent modulation of thioredoxin reductase activity might contribute to sulforaphane mediated inhibition of NF kappaB binding to DNA . ^^^ To further analyze the underlying mechanisms , we focused on the thioredoxin / thioredoxin reductase ( TrxR ) system as a key redox mechanism regulating NF kappaB DNA binding . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Human thioredoxin has been implicated in cancer as a growth stimulator through regulation of DNA replication and growth factor activity , as a modulator of transcription factor activity , and as an inhibitor of apoptosis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The role of enzymatic ( superoxide dismutase ( Cu , Zn SOD , Mn SOD ) , catalase , glutathione peroxidase ) and non enzymatic antioxidants ( Vitamin C , Vitamin E , carotenoids , thiol antioxidants ( glutathione , thioredoxin and lipoic acid ) , flavonoids , selenium and others ) in the process of carcinogenesis as well as the antioxidant interactions with various regulatory factors , including Ref 1 , NF kappaB , AP 1 are also reviewed . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The synthetic gene encoding human enteropeptidase light chain ( L HEP ) was cloned into plasmid pET 32a downstream from the gene of fusion partner thioredoxin immediately after the DNA sequence encoding the enteropeptidase recognition site . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Early effects ( only 1 h of exposure ) of three isothiocyanates ( benzyl , phenylethyl , and sulforaphane ) on nuclear accumulation of thioredoxin , APE / Ref 1 , and transcription factors NF kappaB and Nrf 2 , as well as production of reactive oxygen species ( ROS ) and reduced glutathione levels were examined in human adenocarcinoma Caco 2 cells . ^^^ Nuclear increase of NF kappaB , Nrf 2 , and thioredoxin contents was observed in all isothiocyanate treated cells , whereas the nuclear Ref 1 and cytoplasmic Keap 1 contents were not changed . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Thioredoxin reductase ( TrxR ) reduces thioredoxin ( Trx ) , thereby contributing to cellular redox balance , facilitating the synthesis of deoxy ribose sugars for DNA synthesis , and regulating redox sensitive gene expression . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The mammalian thioredoxin system , comprising the selenoenzyme thioredoxin reductase ( TrxR ) and the 12 kDa protein thioredoxin ( Trx ) , is implicated in thiol mediated antioxidant defense and redox regulatory processes including transcriptional control , DNA synthesis , and apoptosis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The data suggest that , in the absence of thioredoxin , cells can not support the high rate of dNTP synthesis required for efficient DNA synthesis during S phase . ^^^ The results constitute in vivo evidence for thioredoxin being a physiologically relevant electron donor for ribonucleotide reductase during DNA precursor synthesis . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
This study is the first report to demonstrate that the expression of Fe S cluster assembly proteins and the fumarate nitrate reductase regulator decreases the resistance to pressure , while sigma factor ( RpoE ) , lipoprotein ( NlpI ) , thioredoxin ( TrxA ) , thioredoxin reductase ( TrxB ) , a trehalose synthesis protein ( OtsA ) , and a DNA binding protein ( Dps ) promote barotolerance . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Two DNA constructs encoding scytovirin 1 48 ( Cys7Ser ) ( SD 1 ) and 49 95 ( Cys55Ser ) ( SD 2 ) were constructed , and expressed in E . coli , with thioredoxin fused to their N terminus . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Mutation of Phe 102 to Ser in the carboxyl terminal helix of Escherichia coli thioredoxin affects the stability and processivity of T 7 DNA polymerase . ^^^ Processivity of T 7 DNA polymerase relies on the coupling of its cofactor Escherichia coli thioredoxin ( Trx ) to gene 5 protein ( gp 5 ) at 1 : 1 stoichiometry . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In this report , we have used DNA Y junctions as fluorescent scaffolds for EcoRII methyltransferase thioredoxin ( M . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Site directed mutagenesis of cysteines to serines in thioredoxin domains of GRP 58 and cross linking assays revealed that both N and C terminal thioredoxin domains are required for GRP 58 mediated mitomycin C induced DNA cross linking . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Recombinant peroxiredoxin ( FhePrx ) prevented metal catalyzed oxidative nicking of plasmid DNA and detoxified hydrogen peroxide when coupled with Escherichia coli thioredoxin and thioredoxin reductase ( k ( cat ) / K ( m ) =5 . 2 10 10 ( 5 ) M ( 1 ) s ( 1 ) ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
After sperm nuclei were decondensed with EDTA and DTT , biotinylated alpha satellite DNA probes TR 17 and TRX were separately used on preparations from thirteen healthy donors . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
P element mediated transformation using 34 kb of genomic DNA containing the 25 kb trx transcription unit identifies all sequences necessary for normal trx function and limits the 5 ' and 3 ' flanking sequences that could be used in a regulatory capacity to relatively small regions . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
T 7 DNA polymerase activity generated by T 7 gene 5 protein and reduced Trx was lowered by large factors for W31Y , W31A , or W31H compared with W31F or the wild type protein . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In vitro Trx ( SH ) 2 serves as a hydrogen donor for ribonucleotide reductase , an essential enzyme in DNA synthesis , and for enzymes reducing sulfate or methionine sulfoxide . ^^^ E . coli Trx ( SH ) 2 is essential for phage T 7 DNA replication as a subunit of T 7 DNA polymerase and also for assembly of the filamentous phages f 1 and M 13 perhaps through its localization at the cellular plasma membrane . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Furthermore , inhibitors of TRX reductase , 13 cis retinoic acid ( 13 cis RA ) and azelaic acid , inhibited the DNA synthesis of these cells . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
DNA polymerase activity exhibited by the complex of phage T 7 gene 5 protein and Trx ( SH ) 2 is affected only marginally by the W28A substitution , consistent with the buried position of Trp 28 in the protein . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Moreover , not only the ligand binding domain but the DNA binding domain of the GR is also suggested to be a direct target of TRX . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
TRX is multifunctional in that it regulates DNA replication and transcription intracellularly and has various cytokine activities extracellularly associated with cell proliferation , immunological response , etc . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The structure of the active site hydrophobic surface is unchanged by the mutation of Asp 26 and Lys 57 , since DNA polymerase activity in the 1 : 1 complex of the T 7 gene 5 protein and mutant Trx ( SH ) 2 shows similar Kd values ( approximately 5 nM ) for both mutants and wt . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The authors found that Trx acted synergistically with IL 2 and SAC+IL 2 in inducing DNA synthesis in B CLL cells , but not in the normal B cells . ^^^ In clear contrast , SAC+IL 2+ Trx induced DNA synthesis of normal B cells was not inhibited by soluble anti CD 40 MoAb . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Furthermore , specific DNA binding activity of recombinant ER was inhibited by sulfhydryl modifying reagents and restored by the addition of recombinant TRX protein in electrophoretic mobility shift assay . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The conserved nature of regions of similarity between vertebrate forms of MLL and the Drosophila TRX proteins , as well as other domains previously suggested to have a functional role in MLL ( including the AT hooks and the DNA methyltransferase domain ) , was also observed . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
A novel TRX gene fusion vector was developed that contained at the 3 ' end of TRX gene a short DNA sequences encoding a linker peptide ' GSGSGVSQNYPIVQHHHHHH ' , serving not only as a specific HIV 1 protease site but also providing six contiguous histidine ( His ) residues to foreign proteins . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In the present study , we hypothesized that the DNA binding domain ( DBD ) of the GR , which is highly conserved among the nuclear receptors , is also responsible for communication with TRX in vivo . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Moreover , not only the ligand binding domain but the DNA binding domain of the GR is also suggested to be a direct target of TRX . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Results of an in vivo analysis showed that these DNA fragments ( each approximately 400 bp ) contain both TRX and Polycomb group response elements ( TREs and PREs ) and that in the context of the endogenous Ultrabithorax gene , all of these elements are essential for proper maintenance of expression in embryos . ^^^ Dissection of one of these maintenance modules showed that TRX and Polycomb group responsiveness is conferred by neighboring but separable DNA sequences , suggesting that independent protein complexes are formed at their respective response elements . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Collectively , these results suggest that TRX and ASH 1 are components that interact either within trxG protein complexes or between complexes that act in close proximity on regulatory DNA to maintain Ubx transcription . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
TRX regulates the activity of DNA binding proteins , including Jun / Fos and nuclear factor kappaB . ^^^ Electrophoretic mobility shift assay showed that TRX augmented the DNA binding activity of p 53 and also further potentiated Ref 1 enhanced p 53 activity . ^^^ TRX also interacts with an intranuclear reducing molecule redox factor 1 ( Ref 1 ) , which enhances the activity of Jun / Fos . ^^^ Luciferase assay revealed that transfection of TRX enhanced p 53 dependent expression of p 21 and further intensified Ref 1 mediated p 53 activation . ^^^ Overexpression of transdominant negative mutant TRX ( mTRX ) suppressed the effects of TRX or Ref 1 , showing a functional interaction between TRX and Ref 1 . cis Diamminedichloroplatinum ( 2 ) ( CDDP ) induced p 53 activation and p 21 transactivation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Treatment of nuclear extracts with TRX also enhanced DNA NF kappaB complex formation as observed by EMSA , particularly the p 65 subunit . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Our results show that TRX decreased DNA synthesis in a time and dose dependent manner and with a higher efficiency than DFO . ^^^ Mitotic index was also strongly decreased by TRX and , unexpectedly , DFO inhibited mitotic activity to the same extent as TRX , thus there is a discrepancy between the slight reduction in DNA synthesis and a large decrease in mitotic index after DFO treatment . ^^^ The effects of both TRX and DFO on DNA synthesis and mitotic index were reversible after removing the chelators from the culture medium . ^^^ An apoptotic effect of TRX was strongly suggested by analysis of DNA content by flow cytometry , nuclear fragmentation and DNA degradation in oligonucleosomes and confirmed by the induction of a high level of caspase 3 like activity . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
After exposure to IR , nuclear levels of immunoreactive TRX increased , accompanied by an increase in AP 1 DNA binding activity . ^^^ Furthermore , TRX immunoprecipitated from irradiated cells was capable of activating AP 1 DNA binding activity in nonirradiated nuclear extracts . ^^^ Finally , the ability of both TRX and Ref 1 from irradiated cells to stimulate AP 1 DNA binding in nonirradiated nuclear extracts was abolished by chemical oxidation and restored by chemical reduction . ^^^ These results indicate that , in response to IR , TRX and Ref 1 undergo changes in redox state that contribute to the activation of AP 1 DNA binding activity . ^^^ These experiments suggest that a redox sensitive signaling pathway leading from TRX to Ref 1 to the AP 1 complex participates in the up regulation of DNA binding activity in response to ionizing radiation . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
We recently reported that TRX dependent redox regulation plays a crucial role in DNA binding activity of p 53 . ^^^ These results suggest the possible association of TRX with p 53 dependent function including DNA repair in breast cancer . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
TRX promotes DNA binding of transcription factors such as NF kB , AP 1 , p 53 , and PEBP 2 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The genomic DNA encoding TRX was also isolated from S . pombe chromosomal DNA using PCR . ^^^ The heterologous TRX 1C produced from the cDNA clone could be used as a subunit of T 7 DNA polymerase , while the TRX 1G from the genomic DNA could not . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
BACKGROUND : Thioredoxins ( Trx ) are small redox proteins that function as general protein disulphide reductases and regulate several cellular processes such as transcription factor DNA binding activity , apoptosis and DNA synthesis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
TRX promotes DNA binding of transcription factors such as NF kappaB , AP 1 and p 53 . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The electroretinographic amplitudes were higher and the formation of oxidized DNA was lower in trx tg mice compared to wild type mice after light exposure . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Trx G genes encode proteins that modulate transcriptional programs through protein protein interactions that are mediated by PHD and SET domains , and by binding to DNA via A T hooks and methyltransferase homology motifs . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Adenoviral gene transfer of TRX enhanced TRX enzyme activity 2 . 7+ / 0 . 4 fold ( P < 0 . 05 versus cells infected with adenoviral vector expressing green fluorescent protein [ AdGFP ] ) and resulted in a 3 . 8+ / 0 . 5 fold increase of cellular DNA synthesis as detected by methyl [ 3H ] thymidine incorporation ( P < 0 . 001 ) . ^^^ Platelet derived growth factor ( PDGF ) also increased TRX enzyme activity 2 . 5+ / 3 . 3 fold ( P < 0 . 05 versus no stimulation ) and DNA synthesis 6 . 5+ / 0 . 3 fold ( P < 0 . 001 versus no stimulation ) without significant changes in TRX expression . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In contrast , we also found that the nuclear translocation of the redox regulators , Trx and Ref 1 , increase with age , which was suppressed in CR rats . ^^^ Therefore , increases in nuclear Trx and Ref 1 during aging may result in the up regulation of redox sensitive transcription factors , such as NF kappaB or AP 1 , via the interaction of Ref 1 and Trx in a redox dependent manner . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
The samples were processed for immunohistological staining for TRX , which was done by the indirect immunoperoxidase technique . 8 hydoxy 2 ' deoxyguanosine ( 8 OHdG ) , one of the major DNA base modified products , was also detected for an established marker for oxidative stress . ^^^ Myocardial TRX was upregulated in myocarditis and cardiomyopathies with active necrotic stage associated with DNA damage , which may reflect the oxidative stress overload in hemodynamically uncontrolled status . . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
FACS analysis of DNA fragmentation showed that the TRX gene suppressed hepatocyte apoptosis . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Moreover , Trx functions in redox sensitive signal transduction , transcriptional activation of stress response genes , ribonucleotide reduction in synthesis of deoxyribonucleotides for DNA repair , and post injury cell proliferation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Further investigation has indicated that Trx treatment increases the solubility of high molecular weight glycoproteins and causes redistribution of extracellular DNA into the liquid phase of sputum . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Within this CMM , DNA binding sites for the Zeste protein are necessary for the inheritance of active chromatin through Zeste dependent recruitment of BRM , while TRX can bind the CMM even in their absence . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
It is known that Trx enhances the binding of several transcription factors to DNA . ^^^ Thus , oxidants stimulate osteoclastic differentiation by induction of Trx expression , which augments the DNA binding of transcription factors essential for osteoclastic differentiation . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Adenoviral overexpression of human TBP 2 in precursors inhibited osteoclastogenesis and Trx 1 expression , inhibited sRANKL induced DNA binding of AP 1 , but enhanced sRANKL induced DNA binding of NF kappaB . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
METHODS AND RESULTS : The DNA fragments encoding pro subtilisin DFE was amplified and cloned into the vector pET32a to obtain N terminal Trx fusion expression plasmid . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Additionally Trx increased p 53 DNA binding and expression in response to anthracyclines . ^^^ MCF 7 cells expressing mutant redox inactive Trx showed decreased superoxide generation , apoptosis , and p 53 protein and DNA binding . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Ribonucleotide reductase ( RNR ) , a 1 : 1 complex of the non identical R 1 and R 2 subunits , catalyzes the essential de novo synthesis of deoxyribonucleotides for DNA synthesis using electrons from Trx and TrxR . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
In order to elucidate the functions of the mammalian TXN system , we planned to generate Txn knockout mice , and cloned the genomic DNA fragments using the Txn cDNA as a probe . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
Here we report on the full length DNA sequence of the TXN previously isolated from C . fasciculata ( TXN 1 ) . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
It appeared that transcripts involved in the redox status control ( TXN and APEX ) decreased as a function of age , at the basal state and after irradiation , respectively . ^^^
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
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Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10599 and P27695	Pubmed	SVM Score :0.0
NA