| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| METHODS : HIF 1alpha , HIF 2alpha and HIF 1beta were immunolocalized in cycling endometrium . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Reporter analysis using human Flt 1 promoter and gel mobility shift assays showed that the heterodimer of EPAS 1 and aryl hydrocarbon receptor nuclear translocator binds directly to HIF 1 binding site upstream of Flt 1 promoter and transactivates it . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Expression of ARNT , ARNT 2 , HIF 1 alpha , HIF 2 alpha and Ah receptor mRNAs in the developing mouse . ^^^ The recent cloning of ARNT and HIF 1 ( homologues ( ARNT 2 and HIF 2 alpha ) indicates that at least six distinct bHLH PAS heterodimeric combinations can occur in response to a number of environmental stimuli . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Considering these results and that Arnt or Arnt 2 functions as a common partner in the formation of transcriptional regulators with other bHLH / PAS proteins such as AhR , HLF , and HIF 1alpha , the possibility arises that CBP / p300 is extensively involved as a coactivator in the transactivation process by bHLH / PAS ( a conserved sequence motif among Per , Arnt , and Sim ) heterodimer transcription factors through interaction with Arnt or Arnt2 . . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Transcriptionally active heterodimer formation of an Arnt like PAS protein , Arnt 3 , with HIF 1a , HLF , and clock . ^^^ The partner PAS proteins of Arnt 3 were searched for by the two hybrid system in yeast , and HIF 1 alpha , HLF , and Clock among various bHLH / PAS proteins were found . ^^^ Gel mobility shift analysis using nuclear extracts from 293T cells cotransfected with Arnt 3 and HIF 1 alpha ( or HLF ) expression plasmids revealed that these complexes specifically bound the hypoxia response element ( HRE ) . ^^^ Coexpression of Arnt 3 and HIF 1 alpha ( or HLF ) in Arnt deficient c 4 cells enhanced transcription of a reporter gene driven by the HRE sequences . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| AhR and HLF heterodimerize with Arnt as the common partner , and bind to asymmetric E boxes termed XRE and HRE , respectively . ^^^ We expressed AhR Arnt and HLF Arnt in Escherichia coli and used them for DNA binding . ^^^ The dissociation constant of HLF Arnt HRE was 10 . 4 + / 1 . 6 nM . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| As well as HIF 1alpha , HIF 2alpha forms a heterodimeric complex with the aryl hydrocarbon receptor nuclear translocator and upregulates hypoxia inducible genes such as vascular endothelial growth factor . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factors 1alpha and 2alpha ( HIF 1alpha and HIF 2alpha ) are known to dimerize with the aryl hydrocarbon receptor nuclear translocator in mediating this response . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Identification and functional characterization of hypoxia inducible factor 2alpha from the estuarine teleost , Fundulus heteroclitus : interaction of HIF 2alpha with two ARNT 2 splice variants . ^^^ In efforts to characterize mechanisms of hypoxia adaptation in estuarine fish , we have isolated cDNAs encoding HIF subunits from Fundulus heteroclitus ( Atlantic killifish or mummichog ) , including a HIF 2alpha homolog and ARNT2alt , a splice variant of ARNT 2 that contains an additional exon encoding 16 amino acids near the amino terminus . ^^^ HIF 2alpha protein synthesized in vitro binds cognate DNA elements in concert with either Fundulus ARNT 2 splice variant or murine ARNT 1 . ^^^ HIF 2alpha , ARNT 2 , and ARNT2alt mRNAs are expressed in all organs examined . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| HIF 2alpha and HIF 1beta proteins showed a limited range of varieties among different cell lines and different extracellular stimuli . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| In response to hypoxia , stabilized HIF 1alpha and HIF 2alpha proteins bind HIF 1beta and initiate expression of genes that alleviate hypoxic stress , including those promoting neovascularization . ^^^ Because VEGF is highly expressed by metanephric podocytes and collecting ducts , developing mouse kidney was examined for the presence and distribution of HIF 1alpha , HIF 2alpha , and HIF 1beta . ^^^ Both HIF 1 and HIF 2 heterodimers were identified in newborn kidney lysates by immunoprecipitation with HIF 1alpha , HIF 2alpha , and HIF 1beta antibodies and Western blots . ^^^ Immunohistochemistry of newborn kidney demonstrated widespread expression of HIF 1beta protein in nuclei of glomeruli and all tubular segments , whereas HIF 2alpha protein expression was more restricted and localized chiefly to podocytes of developing glomeruli and developing tubules . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| To test whether known HIF 1 and HIF 2 are involved in hypoxia induced heNOS promoter activation , HMEC 1 and HUVEC were transiently transfected with HIF 1alpha and HIF 1beta or HIF 2alpha and HIF 1beta expression vectors . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that HIF 2alpha PAS B binds the analogous ARNT domain in vitro , showing that residues involved in this interaction are located on the solvent exposed side of the HIF 2alpha central beta sheet . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Western blot analyses were used to evaluate HIF 1alpha , HIF 1beta , HIF 2alpha , von Hippel Lindau protein , VEGF , Flt 1 , enolase , and GAPDH . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| The aim of the present study is to investigate whether hypoxia inducible factors ( HIF 1alpha , HIF 2alpha , HIF 1beta ) are involved in enhanced apoptosis in the human placenta from pregnancies with fetal growth restriction ( FGR ) . ^^^ The expressions of HIF 1alpha , HIF 2alpha , and HIF 1beta were examined by immunohistochemical analysis . ^^^ There were no significant differences in the intensity of the staining for HIF 1alpha and HIF 1beta expressions between two groups , while HIF 2alpha was overexpressed in the placenta from pregnancies with FGR group ( P < 0 . 05 ) . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that the C terminal PAS domains of an HIF alpha isoform ( HIF 2alpha ) and ARNT interact in vitro , and that mutations in the solvent exposed beta sheet surface of the HIF 2alpha domain not only disrupt this interaction , but also greatly attenuate the hypoxia response in living cells . ^^^ Here , we have solved the solution structure of the corresponding PAS domain of ARNT and show that it utilizes a very similar interface for the interaction with the HIF 2alpha PAS domain . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| The aryl hydrocarbon receptor nuclear translocator ( Arnt ) , also called Hif 1beta , serves as an obligate heterodimerization partner of Hif 1alpha and Hif 2alpha . ^^^ |
|
| Interacting proteins: Q99814 and P27540 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation studies , yeast two hybrid analysis , and transient transfection experiments demonstrated that MOP 1 and MOP 2 dimerize with ARNT and that these complexes are transcriptionally active at defined DNA enhancer sequences in vivo . ^^^ The expression profiles of the AHR , MOP 1 , and MOP 2 mRNAs , coupled with the observation that they all share ARNT as a common dimeric partner , suggests that the cellular pathways mediated by MOP 1 and MOP 2 may influence or respond to the dioxin signaling pathway . . ^^^ |
|